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From: PESTOEM-at-aol.com
Date: Sat, 1 Jan 2000 14:46:25 EST
Subject: Jeol 1200 Alignment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy New Year to all and especially all who responded to my call for help.
Thanks again. Best wishes for a great year. Sincerely, Peter A.
Stolzenberg,Pesto Inc.



From daemon Sat Jan 01 16:49:40 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Sat, 1 Jan 2000 14:39:45 -0600
Subject: Re: Potential for expansion of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


sounds like a research grant to me. I don't believe the salary of
faculty in Chemistry departments of most major colleges would be
"soft money" requiring grant support. I commend the Society of
Analytical Chemists for trying to encourage this bridge. 20K of seed
money for a new line of research can make an important difference to
a junior faculty member.


} ---------------------------------------------------------------.
}
}
} Hum....so for a 2000 hour year, this works out to be $10 per hour.
} What a gift. And they probably want $30/hour of work in return.
}
} No matter how you package it, all of this babble does not measure
} up to today's standards. Unless SEM, etc. is a obscure and
} diminutive endeavor, I simply do not understand the cost-benefit
} ratio. Maybe this is not an annual salary. OK. Is this in addition
} to an existing income stream? Geeze, I hope it is the latter. But it
} sounds like the position is on-site. So, the candidate gets a full time
} job at McDonald's as well?
}
} All I can say is that I am glad and relieved that I do not have to
} work and try to survive in this type of environment. Welcome to H-2 visas.
}
} gary g.
}
}
} At 08:14 AM 12/31/99 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I just received this notification from the Society for Analytical Chemists
} } of Pittsburgh. Perhaps it will provide an important start for a new chem
} } prof who has an interest in expanding the use of microscopy and/or in
} } walking across the new bridge between microscopy and spectroscopy:
} }
} } "Twenty-first annual Analytical Chemistry Starter Grant Award"
} } The society for Analytical Chemists of Pittsburgh will award one grant of
} } $20,000 to an assistant professor in the field of analytical chemistry.
} } The purpose of this grant is to encourage high-quality, innovative research
} } by a new analytical chemistry professor and to promote the training and
} } development of graduate students in this field. Assistant professors who
} } have accepted a US college or university appoint since December 31, 1996
} } are eligible. Application forms available from:
} } James Chadwick, Chairman
} } Starter Grant Committee
} } Society for Analytical Chemists of Pittsburgh
} } 200 Penn Center Blvd., Suite 332
} } Pittsburgh, PA 15235
} } Ph: 1-800-825-3221, Xt. 208
} } Fx: 412-825-3224
} }
} } Deadline for application receipt: February 29, 2000
} } Award winner announced: May 1, 2000
} }
} }
} } Best regards and welcome to the new millennium!
} } Barbara Foster
} } Consortium President
} } Microscopy/Microscopy Education ...Educating microscopists for greater
} } productivity.
} }
} } 125 Paridon Street Suite 102 Springfield, MA 01118
} } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
} } Visit our web site {http://www.MME-Microscopy.com/education}
} } ******************************************************
} } MME is America's first national consortium providing
} } customized on-site workshops in all areas of
} } microscopy, sample preparation, and image analysis.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Jan 01 17:10:58 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 01 Jan 2000 14:43:01 -0800
Subject: Re: Potential for expansion of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Thanks for the info. I understand what is going on now. sounds like a great opportunity.

I discussed compensation before and don't want to clutter up the list with a repeat. But I
would appreciate an off-list communication which helps me better understand how the
mechanics of SEM and its personnel work in the big picture. I get asked often how to
get into the field. Since I use my SEM for personal research and photography only, I am
not in academia or companys' formal research departments. If I sound ignorant about
this, it is because I am. I work with some high school and upper level pre-high school
students at times using my SEM and LMs. What can be done with the SEM and LMs
turns them on as much as it still does me today. I'm hopelessly hooked.

But I lack facts and figures about this type of career. Rather than speculating about it,
what do you careerists think? Is this a good career? What is involved in getting into
the career at various levels? And what compensation is commensurate at these levels?

All responses will be kept confidential. Use PGP if you need to.

gary g.




At 12:39 PM 1/1/00 , you wrote:
} sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member.
}
}
} } ---------------------------------------------------------------.
} }
} }
} } Hum....so for a 2000 hour year, this works out to be $10 per hour.
} } What a gift. And they probably want $30/hour of work in return.
} }
} } No matter how you package it, all of this babble does not measure
} } up to today's standards. Unless SEM, etc. is a obscure and
} } diminutive endeavor, I simply do not understand the cost-benefit
} } ratio. Maybe this is not an annual salary. OK. Is this in addition
} } to an existing income stream? Geeze, I hope it is the latter. But it
} } sounds like the position is on-site. So, the candidate gets a full time
} } job at McDonald's as well?
} }
} } All I can say is that I am glad and relieved that I do not have to
} } work and try to survive in this type of environment. Welcome to H-2 visas.
} }
} } gary g.
} }
} }
} } At 08:14 AM 12/31/99 , you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } I just received this notification from the Society for Analytical Chemists
} } } of Pittsburgh. Perhaps it will provide an important start for a new chem
} } } prof who has an interest in exd, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.


--CAB25050.946689051/kepler.fmph.uniba.sk--


The subject of the message is: tacky wax

The address to which the message has not yet been delivered is:

cg02-at-gre-wo-staff.greenwich.ac.uk

No action is required on your part. Delivery attempts will continue for
some time, and this warning may be repeated at intervals if the message
remains undelivered. Eventually the mail delivery software will give up,
and when that happens, the message will be returned to you.

} From MAILER-DAEMON Fri Dec 31 19:00 CST 1999
Received: from alta.gre.ac.uk (alta.gre.ac.uk [193.60.48.95]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id TAA01169 for {Microscopy-request-at-sparc5.microscopy.com} ; Fri, 31 Dec 1999 19:00:01 -0600
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for Microscopy-request-at-sparc5.microscopy.com; Sat, 01 Jan 2000 01:00:25 +0000


-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Thanks for the info. I understand what is going on now. sounds like a great opportunity.

I discussed compensation before and don't want to clutter up the list with a repeat. But I
would appreciate an off-list communication which helps me better understand how the
mechanics of SEM and its personnel work in the big picture. I get asked often how to
get into the field. Since I use my SEM for personal research and photography only, I am
not in academia or companys' formal research departments. If I sound ignorant about
this, it is because I am. I work with some high school and upper level pre-high school
students at times using my SEM and LMs. What can be done with the SEM and LMs
turns them on as much as it still does me today. I'm hopelessly hooked.

But I lack facts and figures about this type of career. Rather than speculating about it,
what do you careerists think? Is this a good career? What is involved in getting into
the career at various levels? And what compensation is commensurate at these levels?

All responses will be kept confidential. Use PGP if you need to.

gary g.




At 12:39 PM 1/1/00 , you wrote:
} sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member.
}
}
} } ---------------------------------------------------------------.
} }
} }
} } Hum....so for a 2000 hour year, this works out to be $10 per hour.
} } What a gift. And they probably want $30/hour of work in return.
} }
} } No matter how you package it, all of this babble does not measure
} } up to today's standards. Unless SEM, etc. is a obscure and
} } diminutive endeavor, I simply do not understand the cost-benefit
} } ratio. Maybe this is not an annual salary. OK. Is this in addition
} } to an existing income stream? Geeze, I hope it is the latter. But it
} } sounds like the position is on-site. So, the candidate gets a full time
} } job at McDonald's as well?
} }
} } All I can say is that I am glad and relieved that I do not have to
} } work and try to survive in this type of environment. Welcome to H-2 visas.
} }
} } gary g.
} }
} }
} } At 08:14 AM 12/31/99 , you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } I just received this notification from the Society for Analytical Chemists
} } } of Pittsburgh. Perhaps it will provide an important start for a new chem
} } } prof who has an interest in expanding the use of microscopy and/or in
} } } walking across the new bridge between microscopy and spectroscopy:
} } }
} } } "Twenty-first annual Analytical Chemistry Starter Grant Award"
} } } The society for Analytical Chemists of Pittsburgh will award one grant of
} } } $20,000 to an assistant professor in the field of analytical chemistry.
} } } The purpose of this grant is to encourage high-quality, innovative research
} } } by a new analytical chemistry professor and to promote the training and
} } } development of graduate students in this field. Assistant professors who
} } } have accepted a US college or university appoint since December 31, 1996
} } } are eligible. Application forms available from:
} } } James Chadwick, Chairman
} } } Starter Grant Committee
} } } Society for Analytical Chemists of Pittsburgh
} } } 200 Penn Center Blvd., Suite 332
} } } Pittsburgh, PA 15235
} } } Ph: 1-800-825-3221, Xt. 208
} } } Fx: 412-825-3224
} } }
} } } Deadline for application receipt: February 29, 2000
} } } Award winner announced: May 1, 2000
} } }
} } }
} } } Best regards and welcome to the new millennium!
} } } Barbara Foster
} } } Consortium President
} } } Microscopy/Microscopy Education ...Educating microscopists for greater
} } } productivity.
} } }
} } } 125 Paridon Street Suite 102 Springfield, MA 01118
} } } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
} } } Visit our web site {http://www.MME-Microscopy.com/education}
} } } ******************************************************
} } } MME is America's first national consortium providing
} } } customized on-site workshops in all areas of
} } } microscopy, sample preparation, and image analysis.
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

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From daemon Sat Jan 01 17:50:59 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sat, 1 Jan 2000 17:17:47 -0600
Subject: Re: PDP EDS systems after December 31st

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A simple fix will be to set your date to the year 1972. Since
the days of the week for 1972 = 2000, 1973 = 2001, etc...

It should hold you for a few years (at least it works on my old PDP).

Nestor





From daemon Mon Jan 03 07:35:45 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Sun, 02 Jan 2000 23:04:00 -0500
Subject: WTB: Looking for a Manual, for an AO-860 Microtome....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am looking for a service and or operator's manual (an extra one you may have or a copy)
for an American Optics 860 sliding microtome.

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net



________________________________________________________
1stUp.com - Free the Web®
Get your free Internet access at http://www.1stUp.com



From daemon Mon Jan 03 17:30:19 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Mon, 03 Jan 2000 11:54:50 -0500
Subject: COURSE ANNOUCMENT - Polarized Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am posting this for the NY Microscopical Society.

This is a Very Good Course, at a Great Price.

Best Regards

Joseph Passero
mailto:jp-at-spacelab.net


New York Microscopical Society -- Course Announcement
====================================================

Bernard Friedman Memorial Workshop

Polarized Light Microscopy

April 8, 9, 15 & 16, 2000

An advanced course on polarized light microscopy which will cover the following topics:

The nature of polarized light

The origin and interpretation of interference colors

Birefringence and crystal orientation

The Indicatrix

Compensation and variable compensators

Interference figures and their interpretation

The workshop will consist of two consecutive weekends of lectures and hands on labs to cover
the theoretical and practical aspects of polarized light microscopy.

The course instructors include;

Jan Hinsch of Leica, Inc.

John Reffner of Trace Consulting

N.Y.M.S. Instructor Donald O'Leary.

WHEN: April 8, 9, 15 & 16, 2000 from 10 A.M. to 4 P.M.

WHERE:

New York Microscopical Society Facility
1244 McBride Avenue
West Paterson, NJ.

Phone (973) 812-8377

Web Site URL: http://www.nyms.org

(The facility has free parking and is accessible by public transportation, Information on
car pools and transportation will be provided.)

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership) Lunch and course
materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the Microscope" or are
experienced in microscopy and familiar with the theory of its use.

HOW: Register using the form below. Limited to the first 12 registrants.


For Further Information Contact

Donald O'Leary.

eMail: mailto:donoleary-at-worldnet.att.net

(201) 797 -8849 Voice Phone Number


--------------------------------------------------------------------------------------------------------------

Registration Form

Polarized Light Microscopy

N.Y.M.S. Member ______________________ ($275) Non-Member _______________($295)

Name ___________________________________________________________________

Address __________________________________________________________________

City __________________________________ State ____________________ Zip Code
________________

Phone (W) _______________________ (H) ______________________ eMail
_____________________________



________________________________________________________
1stUp.com - Free the Web®
Get your free Internet access at http://www.1stUp.com



From daemon Mon Jan 03 17:30:21 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 03 Jan 2000 12:56:07 -0500
Subject: Re: Potential for expansion of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom,

Thanks for your on-target observations ...and especially for getting Gary
into a more positive perspective!

Best regards,

Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
At 02:39 PM 1/1/00 -0600, Tom Phillips wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 03 17:30:21 2000



From: Lugosi1936-at-aol.com
Date: Mon, 3 Jan 2000 13:18:13 EST
Subject: Vickers Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking for any information (year of manufacture, company history, etc.)
about a Vickers Instruments, M1500974C microscope. I would especially like
to acquire a copy of the owners manual if possible. Please look at the
following pictures
for further identification.

{A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l
ugosi1936/vic1a.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu
gosi1936/vic2.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu
gosi1936/vic3.jpg {/A}

Thanks for you help,
Dana Grantham





From daemon Mon Jan 03 17:30:22 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 03 Jan 2000 13:20:39 -0500
Subject: LM: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


American Chemical Society, "Applied Optical Microscopy"

3 days of exciting interactive lectures, lab, and demos on all aspects of
Light Microscopy, with a touch of video imaging
Learn how to
-match optics to your application
-interpret images from a variety of contrast techniques
-troubleshoot for artifacts and misinformation
-do simple measurement
-put a camera system on your microscope

New Orleans Hyatt Regency, Mar 10-12,2000
Tuition, books, coffee breaks: $895 for ACS members, $995 for non-members

While many of the examples used will be from materials science, this course
is NOT LIMITED TO CHEMISTS! Biologists are also welcome.

For a syllabus and enrollment information, visit MME's website:
www.MME-Microscopy.com/education (B. Foster is course coordinator)

Please reserve early. This course is given in conjunction with Pittcon,
the biggest analytical meeting in the country. Rooms fill up early.

Best regards .... and welcome to the positive side of Y2K!

Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



From daemon Mon Jan 03 18:31:48 2000



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Mon, 3 Jan 2000 16:03:51 -0800
Subject: CHROMOSOME 5 (5P-)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I am forwarding this from someone who sent this to me. If anyone has any
} ideas for this person, please contact him through his email address which
} is listed.
}
} ML
}
} } From: "rwinn" {rwinn-at-mweb.co.za}
} } To: {wong-at-msg.ucsf.edu}
} } Subject: CHROMOSOME 5 (5P-)
} } Date: Fri, 31 Dec 1999 07:21:48 +0200
} } MIME-Version: 1.0
} } X-Priority: 3
} }
} } DEAR, MEI LEI WONG
} }
} } MY NAME IS RENEY WINN AND I AM MAILING YOU FROM SOUTH AFRICA.
} } I AM 25 YRS OLD AND HAS A BABY BOY OF 14 MTHS.
} }
} } I FOUND OUT THAT HE HAS CRI-DU-CHAT SYNDROME WHICH IS ALSO CALLED CAT-CRY
} } SYNDROME. HE HAS A DELETION ON THE SHORT ARM OF CHROMOSOME 5. HIS
} } DELETION ON THE SHORT ARM IS FROM THE 14.2 MARK WHICH EXTENDS TO THE 15.
} } MARK.
} }
} } I KNOW THAT THIS IS A VERY RARE SYNDROME AND HAVE BEEN IN TOUCH WITH THE
} } SUPPORT GROUPS IN AUSTRALIA AND U.K. HOWEVER..
} } I STILL FEEL THAT I NEED MORE HELP FROM LABRORATRIES OR SCIENTISTS. I AM
} } TRYING TO FIND OUT MORE ABOUT THE SEVERITY OF MY SON'S CONDITION. I AM
} } TRYING TO FIND OUT HOW BADLY HE WILL BE AFFECTED BY HIS DELETION. I WOULD
} } JUST LIKE TO KNOW HOW HIS DELETION SIVERITY WOULD BE CLASIFIED AS. MAYBE
} } ON A SCALE OF 1-10 ONE AS BEING NONE SIVERE AND 10 AS MOST SEVERE. THIS
} } WAY I WOULD BE ABLE TO UNDERSTAND +- HOW SEVERE MY CHILD'S CONDITION IS. I
} } WOULD ALSO APPRECIATE IT IF YOU CAN TELL ME MORE OR LESS WHAT TO EXPECT
} } WITH HIS DELETION.
} }
} } MEI, IF THIS IS NOT SOMETHING YOU CAN HELP ME WITH, COULD YOU PLSE
} } FORWARD THIS MAIL TO SOMEONE WHO COULD PLSE. I AM VERY DESPERATE TO FIND
} } OUT MORE ABOUT THE SEVERITY OF MY CHILDS DELETION. THIS WOULD BE MUCH
} } APPRECIATED.
} }
} } BRGDS
} } RENEY WINN.
} } rwinn-at-mweb.co.z
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu



From daemon Tue Jan 04 18:52:22 2000



From: Mike Southwell :      mjsouth-at-flash.net
Date: Mon, 03 Jan 2000 18:44:05 -0800
Subject: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I know this is a little off the microscopy subject, but in the interest
of encouraging scientific exploration in a young person. I hope you will
indulge me.

I'm looking for materials for my daughter's science project. She is
testing the effectiveness of different mouthwashes in killing bacteria
found in the mouth. Her experiment matrix requires 12 petri dishes with
a bacterial growth medium. I can probably scrounge something to use as
petri dishes, but the growth medium is a problem. I'm not even sure
what it is properly called or what its makeup is. Is it something we
can easily purchase locally, or even better is there a recipe for making
a suitable substitute from ingredients found in the home?

Any and all suggestions will be greatly appreciated.

Michael Southwell
JEOL USA INC.
Austin, TX




From daemon Tue Jan 04 07:29:31 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Tue, 4 Jan 2000 09:33:21 +0100 (MET)
Subject: Metal 2000 in the Ostrava 16-18 May 2000

Contents Retrieved from Microscopy Listserver Archives
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Good morning,

In the Ostrava, Czech 16th-18 May 2000 are organizing
"Metal 2000" - 9th international metallurigcal conference,

about conference look at http://www.tanger.cz/metal2000

best regards

Krzysztof Jan Huebner

{hubner-at-IOd.krakow.pl} :-)



From daemon Tue Jan 04 18:11:43 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Tue, 4 Jan 2000 10:06:47 +0000
Subject: Seeking applicants for a postdoctoral research postion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Research Associate

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The Center for Advanced Thin Film Technology at the University at
Albany - SUNY seeks applicants for a postdoctoral position with
established expertise in the area of analytical electron microscopy.
Demonstrated experience in the development and application of SEM
and/or TEM based analyses, including chemical analysis, is required to
achieve targeted technique development goals and to support an
expanding microscopy base at the Center. Experience with, or interest
in, scanning probe microscopy techniques is also desirable.
Candidates with a Ph.D. in applied physics, materials science and
engineering, chemistry or related fields are encouraged to apply.
This appointment is part of an ongoing multi-year investment in
personnel and new laboratory facilities for thin film technology
research areas at the Center.

The Center is a New York State sponsored high technology research and
development center with a $60M state-of-the-art infrastructure,
including class 1 clean rooms for back end IC manufacturing on 200 mm
wafers, and advanced processing, patterning and characterization
capabilities for single and multi-layered thin films. The Center is
currently assembling a 200 mm wafer facility for MEMS integration. In
addition, a new facility that will house a 25,000 ft2 class 1cleanroom
for 300 mm wafer pilot manufacturing and workforce training is
presently under construction.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203



The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Tue Jan 04 18:11:44 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Tue, 4 Jan 2000 10:30:36 +0000
Subject: Seeking applicants for an analytical support specialist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Analytical Support Specialist

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The New York State Center for Advanced Thin Film Technology at the
University at Albany - SUNY is a fast growing, high technology
research and development program with a mission of supporting industry
and creating new jobs. This position will serve as the primary
infrastructure support person for the Center's scientific and
technical staff.

Job responsibilities include: performing routine maintenance and
calibration of a variety of surface spectroscopic and electron
microscopy instruments; performing analytical work with these
instruments; assisting students with the operation of this equipment;
diagnosing and repairing non-routine problems with the
instrumentation; and maintaining an adequate store of spare parts and
supplies.

The position requires: an associates degree in electronics, computer
science, or related technology and two to four years of directly
relevant experience; a working knowledge of high vacuum systems;
proficiency with related computer hardware/software; demonstrated
ability to work in a high energy, team oriented environment; and
excellent communication and analytical skills. Experience with surface
analysis and/or electron microscopy instrumentation is highly
desirable.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203

The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Tue Jan 04 18:11:43 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 4 Jan 2000 10:05:11 -0500
Subject: Request for EELS data in EMSA Format -Windows Spectra Plotting pr

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I am working on a spectra plotting program in Visual Basic 6 that is just
about ready for general release. I was hoping to put it out there in time
for the M&M MM meeting.

It started out just as a program that would take EMSA formatted EELS data
from Gatan's ELP and convert them into an Excel compatible file so that I
bring the file to a PC. Then I got carried away and used it to try to learn
Visual Basic. Currently, the program will open and overlay up to five
spectra, plot them in linear, log or rescale them and print them. They can
also be copied to the clipboard and pasted in other applications. They can
be re-colored and a couple other things as well. There is even a help
file!

I am trying to make the program more general and make it completely
compatible with the EMMFF standard for all spectra, EDS and EELS alike.I
have a couple of requests for information.

1) I would like to have some EELS spectra of different elements in EMMFF
format to test it out and include in a distribution packet that I can put on
the MAS-LIB. I am particularly interested in transition metal oxides.
Please send them to me if you have them and I could put them in the
deployment file. Who knows, this could be a poor man's EELS atlas.

2) I would like to know how much interest there is in this program.

3) What features would you like in such a program. It doesn't do any
analysis yet, but it may in the future.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Tue Jan 04 18:11:46 2000



From: Howard, Jean M. :      JMHoward-at-rmc.com
Date: Tue, 4 Jan 2000 10:48:26 -0500
Subject: SEM-Refurbishing multi-standards mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have a multi-element standards mount which over time has deteriorated and
become contaminated with salts. Three of the standards have fallen out of
the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in
1982, and it appears that this company no longer exists.
I am interested in finding someone to re-polish the mount and replace the
lost standards. Does anyone know of such a company/person?

Thanks in advance,

Jean M. Howard
Reynolds Metals Company
Materials Characterization-Electron Microscopy
E-mail: jmhoward-at-rmc.com
Office: 804.751.2554






From daemon Tue Jan 04 18:11:46 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 4 Jan 2000 10:53:06 -0500
Subject: RE: SEM - epoxy mountants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina,
I have not been reading all posts so you may have been provided with all the
help you needed, but I thought I would respond just in case...
We do a lot of work with similarly embedded specimens. The problem you have
is very familiar to me, and I believe you are right in your approach to
"Hold the samples in a vacuum for some period of time before putting them
into the scope" This is the best method that I have been able to come up
with. Assuming: 1) the resin is fully cured, 2) you have vacuum
infiltrated the resin into the specimen as thoroughly as practical, and 3)
you have minimized the size of high surface area specimens prior to
embedding, then there is not much more to try. In my experience the
outgassing is most often the result of contaminants within the porosity of
the specimen, and is not the result of the resin. If you still feel the
need to check this out further, you might try an embedding procedure using a
thermosetting resin (such as Bakelite) rather than a thermoplastic (epoxy).


If the sample prep involves polishing, then the source of the contamination
is probably the water or oil lubricant in that procedure. Minimize these
contaminants by preparing the smallest, and best vacuum infiltrated specimen
with as little porosity as possible. I do this by repetitive evacuation/N2
back filling (with heat if possible) prior to embedding. This "clears the
way" for better vacuum infiltration.

I have had polymerization problems with some resin/material combinations,
and I have usually overcome these by choosing a different resin polymer.
Epoxies and acrylics, for example, behave quite differently on various
substrates, so I will try LR White if I am having trouble with epoxy, and
visa versa. If you use LR White for the initial embedding, keep the
reaction volume small, and re-embed in a larger mount if necessary.
I do not know if there are any good references on the subject of vacuum
infiltration for porous materials, but these are the approaches I have
learned to take.
Good Luck!
Brad Huggins

} ----------
} From: Tina Carvalho[SMTP:tina-at-pbrc.hawaii.edu]
} Sent: Tuesday, December 28, 1999 2:23 PM
} To: Microscopy Listserver
} Subject: SEM - epoxy mountants
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} We have a Hitachi S-800 FESEM that is mostly used for biological
} specimens. However, recently we have had a number of planetary geologists
} looking at sections of meteorites mounted on epoxy resin. About the time
} they started using the scope, we started having a number of problems that
} suggest that we are getting some outgassing and contamination. I'm not
} surprised; this happened before when looking at fish ear bones in similar
} resin, despite the resin manufacturer's clain their product was completely
} stable in the high vacuum and under the beam.
}
} My question is for those of you who routinely look at such samples: What
} do you do to minimize the potential problems? Hold the samples in a
} vacuum for some period of time before putting them into the scope? Paint
} the exposed epoxy areas with carbon paint or similar? For the biological
} samples we only require that the specimens be held over dessicant
} overnight, but I suspect that more heroic measures must be taken for these
} samples.
}
} Happy New Year to all!
}
} Tina
}
} **************************************************************************
} **
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} **************************************************************************
} **
}
}



From daemon Tue Jan 04 20:13:28 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Tue, 4 Jan 2000 15:11:21 -0500
Subject: Philips400 LaB6 & Supplies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can someone suggest a reliable manufacturer for LaB6 filaments? I need the
sturdiest, most poor-vacuum-tolerant variety for a Philips EM400T in a
student lab! and I figure that users know best which filaments perform well
in the real world.

Also, I am looking for a source for Philips bulk-sample mounts, the type
used to carry SEM samples for the 6485/STEM system. (I need the mounts, not
the specimen rod.) I need both low-background carbon (preferred) or
beryllium for EDS, as well as standard copper carriers. My usual sources
tell me they have not stocked these items for years. Maybe someone has some
sitting unused in a cabinet somewhere?? or knows of a current supplier.

Offlist replies preferred, so as not to clog up the works... will summarize
and send info to others who are interested.

Thanks for your help.

Ann Hein-Lehman
Trinity College
Hartford, CT
860-297-4289
ann.lehman-at-trincoll.edu




From daemon Tue Jan 04 18:42:23 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Tue, 04 Jan 2000 14:55:19 -0600
Subject: Re: Request for EELS data in EMSA Format -Windows Spectra Plotting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a side question spurred by Scott Walck's post. Is there a simple low
cost program already available that would just allow me to view and print EMSA
format spectra under a Windows PC environment? I found some info. in the
archives regarding the EMMPDL site and the EMMFF software. I even got so far as
to download the sourcecode. But, I don't have a compiler to turn it into an
executable. Even so, I'm not sure from the documentation whether it will do
what I want. Also I came across NIST's DTSA but that's only for Mac.

Any suggestions out there? And if not, then to Scott yes there is some interest
from myself!
Thanks,
Karen Zaruba

"Walck. Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all,
}
} I am working on a spectra plotting program in Visual Basic 6 that is just
} about ready for general release. I was hoping to put it out there in time
} for the M&M MM meeting.
}
} It started out just as a program that would take EMSA formatted EELS data
} from Gatan's ELP and convert them into an Excel compatible file so that I
} bring the file to a PC. Then I got carried away and used it to try to learn
} Visual Basic. Currently, the program will open and overlay up to five
} spectra, plot them in linear, log or rescale them and print them. They can
} also be copied to the clipboard and pasted in other applications. They can
} be re-colored and a couple other things as well. There is even a help
} file!
}
} I am trying to make the program more general and make it completely
} compatible with the EMMFF standard for all spectra, EDS and EELS alike.I
} have a couple of requests for information.
}
} 1) I would like to have some EELS spectra of different elements in EMMFF
} format to test it out and include in a distribution packet that I can put on
} the MAS-LIB. I am particularly interested in transition metal oxides.
} Please send them to me if you have them and I could put them in the
} deployment file. Who knows, this could be a poor man's EELS atlas.
}
} 2) I would like to know how much interest there is in this program.
}
} 3) What features would you like in such a program. It doesn't do any
} analysis yet, but it may in the future.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN





From daemon Tue Jan 04 19:18:31 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 4 Jan 2000 19:13:46 -0600
Subject: Spectra in MSA/MSA Format How to Plot...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



As one of the co-authors of the MSA/MAS File format we
had a goal to make the format simple enough to be
useable in even a simple spreadsheet program.

A number of the major manufacturer's allow you to
translate their data files into this format directly
from their application programs. To use the data
in a spreadsheet, simply specify dual column (x,y)
format for the output file and
then you can import the data files directly into
a MS Excel spreadsheet, or even better a data
graphing program like KaleidaGraph (which runs
on both Mac's & PC's). Then plot to your hearts content.



Nestor
Your Friendly Neighborhood SysOp.





From daemon Tue Jan 04 20:13:27 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Tue, 4 Jan 2000 18:34:58 -0700
Subject: Mat: Cadmium Zinc Telluride thin foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I've been working on thin foils of CZT and I'm looking for some suggestions
to help eliminate artifacts created by ion milling. So far I have polished
side one and etched (to remove polishing damage) with bromine-methanol and
then dimpled side two, ending with 0.1um alumina. I have been successful
obtaining a final sample thickness of about 8 to 10 microns. However, when
I put the samples into the PIPs (Gatan) to complete the thinning process I'm
seeing beam damage. I was hoping that someone might have a different
approach or suggestion that would eliminate the beam damage.
Thanks for your help.
Dorrance




From daemon Wed Jan 05 08:22:43 2000



From: Palatsides, Manuela :      m.palatsides-at-pmci.unimelb.edu.au
Date: Wed, 5 Jan 2000 13:23:00 +1100
Subject: TEM: Staining yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have an investigator who would like to stain just the cell wall of the
yeast S.cerevisae to distinguish it from the cell membrane at the EM level.
Any tips or references willbe appreciated.
Many thanks

Manuela Palatsides
Electron Microscopy
Peter MacCallum Cancer Institute
Locked Bag#1
A'Beckett Street
Melbourne 3000

Telephone: 03 96561244
Fax: 03 96561411
Email: m.palatsides-at-pmci.unimelb.edu.au





From daemon Wed Jan 05 08:22:49 2000



From: Nelson+ Conti :      NelsonC51-at-excite.com
Date: Tue, 4 Jan 2000 21:59:47 -0800 (PST)
Subject: Re: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





On Mon, 03 Jan 2000 18:44:05 -0800, Mike Southwell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello all,
}
} I know this is a little off the microscopy subject, but in the interest
} of encouraging scientific exploration in a young person. I hope you will
} indulge me.
}
} I'm looking for materials for my daughter's science project. She is
} testing the effectiveness of different mouthwashes in killing bacteria
} found in the mouth. Her experiment matrix requires 12 petri dishes with
} a bacterial growth medium. I can probably scrounge something to use as
} petri dishes, but the growth medium is a problem. I'm not even sure
} what it is properly called or what its makeup is. Is it something we
} can easily purchase locally, or even better is there a recipe for making
} a suitable substitute from ingredients found in the home?
}
} Any and all suggestions will be greatly appreciated.
}
} Michael Southwell
} JEOL USA INC.
} Austin, TX
}
}
Michael -
I'm sure that any bacterial growth medium requries agar or, at home,
gelatin. If you were considering making it at home, then buy some clear
gelatin. You'll need to boil water first, though, and then add the gelatin
to make it liquid. Before it cools, pour the gelatin along with the
apprpriate growth medium into the petri plates (typically, a plate holds
about 10 ml of medium). This substance (err, the gelatin) acts primarily as
a hardener for the actual medium. It is generally considered to be
non-nutritive for most bacteria, although I believe that some may consider
it to be nutritious (spelling ?). As for the main nutrients ... I've been
dealing primarily with protozoa, but I'm pretty sure that bacteria can grow
on an extract of boiled lettuce or even wheat grains, etc.
I cannot tell you from my own experience, however, whether a boiled extract
in combination with gelatin does indeed work as a suitable growth medium,
only that I've heard that gelatin works and that, in my experience, a boiled
extract works well for supporting bacterial growth for protozoa.
If, instead, you have access to scientific catalogs, then it's fairly easy
simply to order proteose peptone, glucose, and agar. The peptone is a
standard component of typical "nutrient agar" plates that, I believe, can be
bought commercially, and the glucose may be needed as a sugar source and
carbon source (?). The agar serves to harden the medium much like gelatin is
supposed to.
I hope this helps.
Nelson Conti
[a graduate student formerly from San Francisco State University with a M.A.
degree in microbiology & a B. A. degree from UC Davis in Bacteriology ]





_______________________________________________________
Visit Excite Shopping at http://shopping.excite.com
The fastest way to find your Holiday gift this season




From daemon Wed Jan 05 08:22:53 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 5 Jan 2000 00:07:03 -0800 (PST)
Subject: Re: SEM - epoxy mountants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear tina and others,
My experience with samples you have described makes me head for the
nearest exit! However, I have looked at samples such as expanded teflon,
and vascular castings mounted on nothing more than carbon sticky tabs.
Frequently I have clientes who do not want samples to be gold coated and
consequently, charging is an issue.

I frequently examine this specimens at an extremely reduced kV and adjust
the spot size and working distance to help in obtaining the best
resolution. The parameters will be different for a FESEM: I am using a
tungsten system. I would think a coating of colloidal carbon or silver to
ground would help under any circumstances. Any thoughts? Good luck!

Ken Tiekotter




On Tue, 28 Dec 1999, Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} We have a Hitachi S-800 FESEM that is mostly used for biological
} specimens. However, recently we have had a number of planetary geologists
} looking at sections of meteorites mounted on epoxy resin. About the time
} they started using the scope, we started having a number of problems that
} suggest that we are getting some outgassing and contamination. I'm not
} surprised; this happened before when looking at fish ear bones in similar
} resin, despite the resin manufacturer's clain their product was completely
} stable in the high vacuum and under the beam.
}
} My question is for those of you who routinely look at such samples: What
} do you do to minimize the potential problems? Hold the samples in a
} vacuum for some period of time before putting them into the scope? Paint
} the exposed epoxy areas with carbon paint or similar? For the biological
} samples we only require that the specimens be held over dessicant
} overnight, but I suspect that more heroic measures must be taken for these
} samples.
}
} Happy New Year to all!
}
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
}




From daemon Wed Jan 05 08:22:57 2000



From: TQ23TEAM-at-LHT.DLH.DE
Date: Wed, 5 Jan 2000 10:30:53 +0100
Subject: Re: SEM-Refurbishment multi-standard mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have a multi-element standards mount which over time has deteriorated and
become contaminated with salts. Three of the standards have fallen out of
the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in
1982, and it appears that this company no longer exists.
I am interested in finding someone to re-polish the mount and replace the
lost standards. Does anyone know of such a company/person?

Thanks in advance,

Jean M. Howard
Reynolds Metals Company
Materials Characterization-Electron Microscopy
E-mail: jmhoward-at-rmc.com
Office: 804.751.2554

Dear Jean,

Plano W. Plannet GmbH is a supplier for electron microscopy. This company
offers refurbishment of SEM standards.
The company is situated in Germany.

Address: Ernst-Befort-Str.12
D-35578 Wetzlar

e-mail: plano-at-t-online.de, or plano-at-plano-em.com
http://www.plano-em.com

You might contact them to see if they can help you with your problem.

best regards
Mit freundlichen Gr٤en

Detlef Warmbold
Geb. 381 Raum 2152
Tel. / Fax +49/40-5070-3962/1411
E-mail TQ23Team-at-LHT.DLH.DE



From daemon Wed Jan 05 08:53:23 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Wed, 5 Jan 2000 09:44:01 +0000
Subject: Seeking applicants for a postdoctoral research position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Research Associate

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The Center for Advanced Thin Film Technology at the University at
Albany - SUNY seeks applicants for a postdoctoral position with
established expertise in the area of analytical electron microscopy.
Demonstrated experience in the development and application of SEM
and/or TEM based analyses, including chemical analysis, is required to
achieve targeted technique development goals and to support an
expanding microscopy base at the Center. Experience with, or interest
in, scanning probe microscopy techniques is also desirable.
Candidates with a Ph.D. in applied physics, materials science and
engineering, chemistry or related fields are encouraged to apply.
This appointment is part of an ongoing multi-year investment in
personnel and new laboratory facilities for thin film technology
research areas at the Center.

The Center is a New York State sponsored high technology research and
development center with a $60M state-of-the-art infrastructure,
including class 1 clean rooms for back end IC manufacturing on 200 mm
wafers, and advanced processing, patterning and characterization
capabilities for single and multi-layered thin films. The Center is
currently assembling a 200 mm wafer facility for MEMS integration. In
addition, a new facility that will house a 25,000 ft2 class 1cleanroom
for 300 mm wafer pilot manufacturing and workforce training is
presently under construction.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203

The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Wed Jan 05 09:03:24 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Wed, 5 Jan 2000 09:47:27 +0000
Subject: Seeking applicants for an analytical support position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Analytical Support Specialist

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The New York State Center for Advanced Thin Film Technology at the
University at Albany - SUNY is a fast growing, high technology
research and development program with a mission of supporting industry
and creating new jobs. This position will serve as a primary
infrastructure support person for the Center's scientific and
technical staff.

Job responsibilities include: performing routine maintenance and
calibration of a variety of surface spectroscopic and electron
microscopy instruments; performing analytical work with these
instruments; assisting students with the operation of this equipment;
diagnosing and repairing non-routine problems with the
instrumentation; and maintaining an adequate store of spare parts and
supplies.

The position requires: an associates degree in electronics, computer
science or related technology and two to four years of directly
relevant experience; a working knowledge of high vacuum systems;
proficiency with related computer hardware/software; demonstrated
ability to work in a high energy, team oriented environment; and
excellent communication and analytical skills. Experience with surface
analysis and/or electron microscopy instrumentation is highly
desirable.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203

The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Wed Jan 05 08:23:13 2000



From: Geoff Williams :      Geoffrey.Lloyd.Williams-at-cmich.edu
Date: Wed, 05 Jan 2000 08:30:43 -0500
Subject: GEN: ATTN: Lab Managers W/SEM&EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a question for all of the SEM microscope labs with EDS.

What is the percent of Biological use that the EDS gets in your
facility?

This is going to be my first semester teaching a Biological EDS class.
The facility here sees very little, if any, biological EDS projects. It
is used here as in other places I am familiar with nearly 100% non
biological applications.

I would like as many responses as possible so I can give a
representative summary to the students in the class. A short reply
directly to me ( Geoffrey.Lloyd.Williams-at-cmich.edu or willi1gl-at-cmich.edu
) would be much appreciated. A rough % bio use, most popular bio
samples (plants or animals, type of organisms . . .) and most common
analysis (Quantitative, Qualitative, Dot mapping. . .).

Thank you in advance. If there is sufficient interest I would be
willing to put a summary up on the list for all.

-Geoff W.

--
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Wed Jan 05 08:53:22 2000



From: Gillmeister, Russ :      RGillmeister-at-sdms.usa.xerox.com
Date: Wed, 5 Jan 2000 09:15:28 -0500
Subject: Re: SEM - epoxy mountants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken, If you have a vacuum evaporator Ken, I would try indirect carbon. Most
metals will evaporate directionally only. Carbon will also evaporate
indirectly (around corners) although at a reduced thickness. The advantage
of this type of evaporation is the carbon will coat very convoluted surfaces
which are not line of site. Another advantage is the carbon will add very
little structure to your sample at high magnifications. Additionally heating
of the sample can be reduced due to shading of the source from the sample.
One caveat is, as you know, carbon is a very inefficient secondary producer
and the carbon will not have the efficiency of gold. Indirect carbon, with a
proper thickness, will prevent charging though. Just put a line of site
shield between your source and sample while keeping it as small as possible.
Good luck. Russ Gillmeister, Xerox

-----Original Message-----
} From: Ken Tiekotter [mailto:tiekotte-at-up.edu]
Sent: Wednesday, January 05, 2000 3:07 AM
To: Tina Carvalho
Cc: Microscopy Listserver


Dear tina and others,
My experience with samples you have described makes me head for the
nearest exit! However, I have looked at samples such as expanded teflon,
and vascular castings mounted on nothing more than carbon sticky tabs.
Frequently I have clientes who do not want samples to be gold coated and
consequently, charging is an issue.

I frequently examine this specimens at an extremely reduced kV and adjust
the spot size and working distance to help in obtaining the best
resolution. The parameters will be different for a FESEM: I am using a
tungsten system. I would think a coating of colloidal carbon or silver to
ground would help under any circumstances. Any thoughts? Good luck!

Ken Tiekotter




On Tue, 28 Dec 1999, Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} We have a Hitachi S-800 FESEM that is mostly used for biological
} specimens. However, recently we have had a number of planetary geologists
} looking at sections of meteorites mounted on epoxy resin. About the time
} they started using the scope, we started having a number of problems that
} suggest that we are getting some outgassing and contamination. I'm not
} surprised; this happened before when looking at fish ear bones in similar
} resin, despite the resin manufacturer's clain their product was completely
} stable in the high vacuum and under the beam.
}
} My question is for those of you who routinely look at such samples: What
} do you do to minimize the potential problems? Hold the samples in a
} vacuum for some period of time before putting them into the scope? Paint
} the exposed epoxy areas with carbon paint or similar? For the biological
} samples we only require that the specimens be held over dessicant
} overnight, but I suspect that more heroic measures must be taken for these
} samples.
}
} Happy New Year to all!
}
} Tina
}
}
****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
*
} * Biological Electron Microscope Facility * (808) 956-6251
*
} * University of Hawaii at Manoa *
http://www.pbrc.hawaii.edu/bemf*
}
****************************************************************************
}
}
}



From daemon Wed Jan 05 10:33:07 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 5 Jan 2000 10:15:07 -0600
Subject: SEM: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Due to some recent high-resolution requirements in our lab, I find myself
having to go back to Sputter Coating 101 (after years of just putting
specimens in the coater and turning it on without a second thought!). We
find ourselves in need of very thin coatings with as little structure as
possible, in order to image samples down in the nanometer range.

We have a chromium coater, but often need to revisit samples days or weeks
after the initial coating. The oxidation problem rears its ugly head. We
intend to purchase a platinum target, but don't yet have one, so we're
experimenting with our venerable Au/Pd coater.

My questions are:

1) I seem to remember a string on this listserver suggesting that lower
deposition currents yield finer coating structure. Is this right? Does a
low deposition current for a longer time yield a finer coating than a higher
current for a shorter time? (I'm running some tests to check this, but
would be very interested in others' experiences, too.)

2) Deposition current can be controlled by the initial current setting
(i.e., the knob on the machine) and by the argon flow through the chamber.
Is there any difference in the coating when adjusting the deposition current
by either of these two ways?

3) Charts I have seen indicate that deposition current is directly
proportional to coating rate. Is the same true for coating time? I.e., is a
one minute coating twice as thick as a 30 sec. coating? It would seem so
intuitively, but you know what they say about the word "assume".

My apologies if these are very basic questions, but, like I said, back to SC
101!

I'll be happy to summarize the responses for anyone who is interested.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine Bldg.
University of Missouri
Columbia, MO 65211
(573)882-8304
tindallr-at-missouri.edu
http://www.biotech.missouri.edu/emc/




From daemon Wed Jan 05 10:33:07 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 05 Jan 2000 08:30:38 -0800
Subject: Re: Mat: Cadmium Zinc Telluride thin foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

You can probably use Agar, this is commonly used to grow yeast cultures for
brewing. This is a vegetable based gelatin like substance. You can buy it
at some organic type stores in the bulk section (you don't need much
probably 10 tbsp) it costs about $100/ounce.

I don't remember the exact proportions but I think you use something like
1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will
probably be fine). You just get it al boiling for 5-10 min to sterilize it
and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put
it in the fridge and it should harden in 10-15 min. If not, then my
proportions are off.

After it is hard, just wipe some saliva on the media and watch the green and
red goo grow.

Good luck
-andrew

----- Original Message -----
} From: Mike Southwell {mjsouth-at-flash.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 03, 2000 7:44 PM


Dear Dorrance,
It seems to me that the etching step is redundant. The ion-milling should
remove any polishing deformation and the etching will introduce surface
profiling. I recall that the CZT is very soft and may require some
modification to the ion beam voltage or current to reduce damage. Sounds
like you are close to getting good results.
At 06:34 PM 1/4/00 -0700, you wrote:
}
} Hi,
} I've been working on thin foils of CZT and I'm looking for some suggestions
} to help eliminate artifacts created by ion milling. So far I have polished
} side one and etched (to remove polishing damage) with bromine-methanol and
} then dimpled side two, ending with 0.1um alumina. I have been successful
} obtaining a final sample thickness of about 8 to 10 microns. However, when
} I put the samples into the PIPs (Gatan) to complete the thinning process I'm
} seeing beam damage. I was hoping that someone might have a different
} approach or suggestion that would eliminate the beam damage.
} Thanks for your help.
} Dorrance
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Jan 05 11:42:58 2000



From: Donna Wagahoff :      DWAGAHOFF-at-siumed.edu
Date: Wed, 5 Jan 2000 11:08:12 -0600
Subject: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have always kept our osmium solutions in glass containers. However, we
are in the process of evaluating our safety procedures and discussed the
idea of increasing the safety of the transport of osmium from the
refrigerator to the fume hood by putting the osmium solution in plastic
containers. (If they are dropped, they would not break and cause the danger
of a spill outside the hood.)
Does anyone have experience with osmium stored in plastic? Any comments
about this particular subject or any of your safety with osmium procedures
are welcomed.
Thanks.

Donna Wagahoff
SIU School of Medicine
PO Box 19627
Springfield, IL 62794-9627
217-782-0898
fax217-524-3227



From daemon Wed Jan 05 12:12:54 2000



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Wed, 05 Jan 2000 10:07:45 -0800
Subject: Re: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

This experiment is very popular in the science fair circuit; it is only
slightly less popular than "What kind of music do plants like best?".
Moreover (as a topic RELEVANT TO THE LIST) van Leeuwenhoek showed more than
300 years ago, BY MICROSCOPY, that the experiment as usaully performed is
invalid as a test of mouthwash efficacy in situ. You might want to consult
his observations on the topic.

Prepared agar media can be purchased from educational scientific supply
houses such as Carolina Biologicals or Ward's, both of which maintain web
sites. They both offer "instant" formulations designed for preparation of
Petri dishes without autoclaving. You would want a medium capable of
supporting growth of Streptococcus species, which are an important
component of the oral microflora. The streptococci are somewhat fastidious
in their nutritional requirements. Therefore, select something rich in
organic nutrients such as amino acids. Tryptic Soy Medium would probably
work best. The proteose peptone/glucose composition suggested by a previous
respondent would also probably work. Lettuce or wheat grain extract would
probably not give satisfactory results.If you wish to make your own medium
at home from local sources, I suggest table sugar, beef bullion (more
concentrated than in culinary use) and agar (often available at health food
stores). A pressure cooker would help to ensure sterility during
preparation. Be advised that many of the bacteria in the oral cavity are
obligate anaerobes; they will not grow on any medium if it is in contact
with the atmosphere.

Best wishes for the success of your daughter's work!
Mike Dalbey
Biology Dept.
University of California
Santa Cruz, CA 95064

831-459-3674



From daemon Wed Jan 05 13:12:48 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 05 Jan 2000 13:34:49 -0500
Subject: Fine SEM coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Randy Tindall wrote:
=================================================
{snip}
We find ourselves in need of very thin coatings with as little structure
as possible, in order to image samples down in the nanometer range.

We have a chromium coater, but often need to revisit samples days or weeks
after the initial coating. The oxidation problem rears its ugly head. We
intend to purchase a platinum target, but don't yet have one, so we're
experimenting with our venerable Au/Pd coater.

{snip}
===================================================
One (maybe the only) solution to the problem is to coat with osmium metal in
the osmium plasma coater. Pt grain size can be on the same size magnitude
as your nanostructures. You can get information about this coater on our
website at URL
http://www.2spi.com/catalog/osmi-coat.html

One does not need to worry about grain size because it is an (apparently
completely) amorphous layer and since it is a precious group metal, it has
the intertness of Au and Pt and will essentially last forever.

The physics of the process is explained in the US Patent #5855682 which can
be clicked to from the above URL.

We would be happy to coat some demo samples for you in our demo unit at any
time, just let us know.

Disclaimer: SPI Supplies distributes the line of Osmium Plasma Coaters,
manufactured by Nippon Laser and Electronics Ltd.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Jan 05 13:12:48 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 05 Jan 2000 13:34:49 -0500
Subject: Fine SEM coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Randy Tindall wrote:
=================================================
{snip}
We find ourselves in need of very thin coatings with as little structure
as possible, in order to image samples down in the nanometer range.

We have a chromium coater, but often need to revisit samples days or weeks
after the initial coating. The oxidation problem rears its ugly head. We
intend to purchase a platinum target, but don't yet have one, so we're
experimenting with our venerable Au/Pd coater.

{snip}
===================================================
One (maybe the only) solution to the problem is to coat with osmium metal in
the osmium plasma coater. Pt grain size can be on the same size magnitude
as your nanostructures. You can get information about this coater on our
website at URL
http://www.2spi.com/catalog/osmi-coat.html

One does not need to worry about grain size because it is an (apparently
completely) amorphous layer and since it is a precious group metal, it has
the intertness of Au and Pt and will essentially last forever.

The physics of the process is explained in the US Patent #5855682 which can
be clicked to from the above URL.

We would be happy to coat some demo samples for you in our demo unit at any
time, just let us know.

Disclaimer: SPI Supplies distributes the line of Osmium Plasma Coaters,
manufactured by Nippon Laser and Electronics Ltd.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Jan 05 12:42:51 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 5 Jan 2000 12:41:26 -0600
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Agar can be purchased from some Asian stores at much cheaper rate if you can find them. It is made into strands, so, don't expect powder form.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "Andrew Cahill" {cahill-at-colorado.edu} 01/05 11:15 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mike,

You can probably use Agar, this is commonly used to grow yeast cultures for
brewing. This is a vegetable based gelatin like substance. You can buy it
at some organic type stores in the bulk section (you don't need much
probably 10 tbsp) it costs about $100/ounce.

I don't remember the exact proportions but I think you use something like
1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will
probably be fine). You just get it al boiling for 5-10 min to sterilize it
and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put
it in the fridge and it should harden in 10-15 min. If not, then my
proportions are off.

After it is hard, just wipe some saliva on the media and watch the green and
red goo grow.

Good luck
-andrew

----- Original Message -----
} From: Mike Southwell {mjsouth-at-flash.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 03, 2000 7:44 PM


Agar can be purchased from some Asian stores at much cheaper rate if you can find them. It is made into strands, so, don't expect powder form.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "Andrew Cahill" {cahill-at-colorado.edu} 01/05 11:15 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mike,

You can probably use Agar, this is commonly used to grow yeast cultures for
brewing. This is a vegetable based gelatin like substance. You can buy it
at some organic type stores in the bulk section (you don't need much
probably 10 tbsp) it costs about $100/ounce.

I don't remember the exact proportions but I think you use something like
1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will
probably be fine). You just get it al boiling for 5-10 min to sterilize it
and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put
it in the fridge and it should harden in 10-15 min. If not, then my
proportions are off.

After it is hard, just wipe some saliva on the media and watch the green and
red goo grow.

Good luck
-andrew

----- Original Message -----
} From: Mike Southwell {mjsouth-at-flash.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 03, 2000 7:44 PM


I store my osmium in a glass bottle with plastic cap (Schott bottle -
orange cap - commonly used for tissue culture work). The inside
portion of the cap turns black quite rapidly but the solution is
stable for months at room temperature. I store this glass bottle
inside an aluminum-foiled plastic container in my fume hood at room
temp. The clear plastic of this outer container gets translucent
black within weeks no matter how carefully I seal the glass bottle.
I think the storage of osmium in any single container (except a
sealed ampule) in the refrigerator is foolish and unnecessary. Due
to University regulations, we are required to store our aldehyde
fixatives and other toxic chemicals inside the refrigerator in
"secondary containment" containers. We keep the glass bottles of
aldehydes in small plastic containers and transport them this way to
the fume hood for use.

}
}
}
} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Jan 05 13:22:45 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 05 Jan 2000 14:16:01 -0500
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donna Wagahoff wrote:

} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.

Dear Donna,
How about putting the glass containers inside plastic containers?
There are also padded and styrofoam containers which you could use for
the transportation step. These can usually be made out of waste packaging
material--better than sending it to a landfill.
Yours,
Bill Tivol




From daemon Wed Jan 05 13:32:43 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 5 Jan 2000 11:20:43 -0800 (PST)
Subject: Re: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I know this is a little off the microscopy subject, but in the interest
} of encouraging scientific exploration in a young person. I hope you will
} indulge me.
}
} I'm looking for materials for my daughter's science project. She is
} testing the effectiveness of different mouthwashes in killing bacteria
} found in the mouth. Her experiment matrix requires 12 petri dishes with
} a bacterial growth medium. I can probably scrounge something to use as
} petri dishes, but the growth medium is a problem. I'm not even sure
} what it is properly called or what its makeup is. Is it something we
} can easily purchase locally, or even better is there a recipe for making
} a suitable substitute from ingredients found in the home?
}
} Any and all suggestions will be greatly appreciated.
}
Mike -

You're a supportive parent! I agree with you that purchasing prepared
microbiological groth media doesn't have as much learning potential as
starting from scratch, but it's undeniably easier. And probably cheaper.
How old is she? Since you obviously don't know any microtechnique, I worry
about the adequacy of her "experiment matrix". Some degree of sterile
technique is required, implying the use of an alcohol lamp-sterilized wire
loop.

If you want to cook your own, you'll need a pressure cooker and the
instructions available in Zook et al., "The Microcosmos guide to exploring
microbial space" (see the MICRO bibliography! URL below). I can send you
photocopied pages, but if this is a major project you may want the book.
Or you can buy prepared sterile plates (and the agarose that you'd need for
home cooking) from any large biological supply house, such as Carolina
Biological (800-334-5551). You may even have a medical supply source in
town.

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Wed Jan 05 15:32:24 2000



From: rfelten-at-Macdermid.com
Date: Wed, 5 Jan 2000 16:16:31 -0500
Subject: Looking for a Contract laboratory that does AFM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Rick Felten-at-MACDERMID
01/05/2000 04:16 PM
I have a couple of Ni/Au Samples that I need analyzed using AFM. The
conductive samples would need to be scanned over a 10 X 10 micron region.
Were are located in Waterbury Ct and the closer to us the better.

Any Help would be appreciated.

Ric





From daemon Wed Jan 05 16:02:18 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 5 Jan 2000 13:43:33 -0800
Subject: Image distortion correction?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI:

Is there a way to remove a moderate barrel distortion from an image? I have
images taken with a 17 mm wide angle lens that are slightly distorted and
wish to make some area measurements from them.

I have a picture of a grid of known size so it seems like there should be a
way to 'stretch' the picture into shape in something like Photoshop, I just
don't know where to look. Any help or ideas?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Wed Jan 05 16:12:20 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 5 Jan 2000 12:04:59 -1000 (HST)
Subject: TEM, Sectioning, Need Ultracut belt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all-

The drive belt on our Reichert (Leica) Ultracut E broke last night,
leaving a number of people in a state of panic. Leica does not have any
of these in the US, and the Vienna group is still on holiday.

I would appreciate and forever be indebted to anyone who could quickly
send me the appropriate belt, which I could either pay for (list $38.34,
plus shipping) or replace when ours come in.

Mahalo!
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Wed Jan 05 16:32:18 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 5 Jan 2000 17:22:52 -0500
Subject: RE: Spectra in MSA/MSA Format How to Plot...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor,
The Gatan ELP option for MSA formatted files outputs the files to 5 columns
serial. There are other ASCII options, but they are not MSA format. I have
found that Noran does the same thing in our new system. It is not easy to
parse the data for a spreadsheet when it is in that format. I would have
been happy if they had done it with the two column option that is available
in the standard or if they simply used a single column. Once it is in that
format, it's easy to do in a spreadsheet. With multiple columns, I had to
go into the file with a word processor, take out the hard returns at the end
of each row, and then replace all of the commas with hard returns. Then I
could open them easily in the spreadsheet. That's how I got started with
the Basic program -simply to read them in and output them to a two column
text with the MSA format. Then I got carried away with VB.

-Scott



} -----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-Sparc5.Microscopy.Com]
} Sent: Tuesday, January 04, 2000 8:14 PM
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Spectra in MSA/MSA Format How to Plot...
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} As one of the co-authors of the MSA/MAS File format we
} had a goal to make the format simple enough to be
} useable in even a simple spreadsheet program.
}
} A number of the major manufacturer's allow you to
} translate their data files into this format directly
} from their application programs. To use the data
} in a spreadsheet, simply specify dual column (x,y)
} format for the output file and
} then you can import the data files directly into
} a MS Excel spreadsheet, or even better a data
} graphing program like KaleidaGraph (which runs
} on both Mac's & PC's). Then plot to your hearts content.
}
}
}
} Nestor
} Your Friendly Neighborhood SysOp.
}
}
}



From daemon Wed Jan 05 17:42:07 2000



From: mykkb-at-juno.com
Date: Wed, 5 Jan 2000 18:28:19 -0500
Subject: Re:Bio Bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a Bacterial Growth Medium Kit for grades 3-6 in the Carolina
K-6 Science Catalog ( I have the Fall 1998 Catalog). It contains 2 cans
of Beef Browth, 2 Boxes of Unflavored Gelatin, Multivitamins, 20 sterile
Petri Dishes and 10 sterile Cotton-Tipped Applicators. It recommends the
use of a pressure cooker or autoclave for "complete" sterility.
It costs $26.43 per kit (1998 price) Phone: 1-800-334-5551
Most of the items are available at the supermarket. The petri dishes and
sterile applicators are more difficult to get. A local college
microbiology department might give a hand or even some sterile Nutrient
Agar Plates.

Mike Baxter
Lehman College




From daemon Wed Jan 05 17:42:09 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Wed, 5 Jan 2000 17:37:02 -0600
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Donna,

Whenever I tried storing osmium solutions in plastics they always reacted
with the plastic causing it to blacken. This took place even in Teflon
altho at a much slower rate.

Why not use a glass that has been coated with plastic and rendered
breakproof. I see that many chemicals (like acids) come in such reagent
bottles.

Hint: maybe one of the EM vendors may know about this.

John



} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################





From daemon Wed Jan 05 18:12:04 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 05 Jan 2000 15:41:36 -0800
Subject: Mag. Calibration for JEOL 5800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

The newer SEMs require software access to do some fundamental
adjustments: magnification, high voltage, crt brightness, etc.

I am attempting to calibrate the magnification on a JEOL 5800 SEM. I am
guessing this requires a sequence of keystrokes to access the computer
in "service" mode. Does anyone have the keystroke sequence or know of
the calibration procedure for the JEOL 5800?

Thank You,
Earl Weltmer




From daemon Thu Jan 06 07:35:21 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 6 Jan 2000 10:51:49 +1000
Subject: RE: SEM-Refurbishing multi-standards mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We could quote on that and certainly, if it involved repolishing and re-coating
only this would be worthwhile. In this case the separated standards would need
to be identified, remounted and thoroughly polished. If much thickness is lost
during the polishing there may be a problem with other standards.
Certainly its possible but I expect that the cost will come close to our new
standard blocks, complete with micro-engraving. I suggest that you check it out
in our online.
Disclaimer: ProSciTech supplies WDS/EDS standards.

Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, January 05, 2000 1:48 AM, Howard, Jean M. [SMTP:JMHoward-at-rmc.com]
wrote:
}
} Dear listers,
}
} I have a multi-element standards mount which over time has deteriorated and
} become contaminated with salts. Three of the standards have fallen out of
} the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in
} 1982, and it appears that this company no longer exists.
} I am interested in finding someone to re-polish the mount and replace the
} lost standards. Does anyone know of such a company/person?
}
} Thanks in advance,
}
} Jean M. Howard
} Reynolds Metals Company
} Materials Characterization-Electron Microscopy
} E-mail: jmhoward-at-rmc.com
} Office: 804.751.2554
}
}
}




From daemon Wed Jan 05 20:51:40 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 5 Jan 2000 21:29:07 -0500 (EST)
Subject: old Kinney evap instruc bk avail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Some time ago I sent out a Kinney High Vacuum manual. There were 4
interested parties; one got the original; others, copies. I have now
found a 2nd one of these antiques, and would be happy to send it to the
highest bidder (i.e., free to the first to reply). The date in the front is
listed as June, 1961.

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Thu Jan 06 08:07:04 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Thu, 6 Jan 2000 07:59:23 -0600
Subject: RE: Spectra in MSA/MSA Format How to Plot...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott etal...

Perhaps it is time to point out to some of the Manufacturers that they should
implement the MSA/MAS standard with an option to specify
the number of columns in the output file ( like the demo/test
copy does). That would be in the spirit of how the format
was originally designed so that "additional" programs code
would not have to be written. They generally listen to customers
so speak your mind!

In the mean time I'll look into also putting a version of the
demo translator on-line.


(Grinning Devilishly)

Nestor
Your Friendly Neighborhood SysOp

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From daemon Thu Jan 06 18:00:58 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Thursday, January 06, 2000 4:30 PM
Subject: SEM- service companies in Mexico

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Lord.......So this is what NAFTA brings us to.......Third world
companies undermining real science with a New Brand of Burger King products
and services? Care for an Enchilada while you wait for your SEM
images?.....$5.95 please.......

Just a cynical comment that is much closer to the truth than I care to think
about.
-----Original Message-----
} From: Paul Rennie (KIDDE) {Paul.Rennie-at-kidde-hq.com}
To: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}


Dear All,

Having just purchased a new SEM, our old instrument, a Cambridge S200 is
destined to be sent to one of our companies in Cuidad Victoria, Mexico
(Although I'm trying to persuade management that it's not worth it).
Although we will probably be installing the instrument ourselves, I would
welcome details of any service companies in Mexico. Additionally, who sells
consumables within Mexico, or is it best to purchase over the border in the
USA.

Thanking you in advance.

Paul Rennie

Kidde International Research
Mathisen Way
Colnbrook
Slough
Berks SL3 0HB
UK

Tel: 44 (0) 1753 683245
Fax: 44 (0) 1753 683810
http://www.kidde-int.com





From daemon Thu Jan 06 18:00:52 2000



From: Paul Rennie (KIDDE) :      Paul.Rennie-at-kidde-hq.com
Date: Thu, 6 Jan 2000 15:54:44 +0000
Subject: SEM- service companies in Mexico

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Having just purchased a new SEM, our old instrument, a Cambridge S200 is
destined to be sent to one of our companies in Cuidad Victoria, Mexico
(Although I'm trying to persuade management that it's not worth it).
Although we will probably be installing the instrument ourselves, I would
welcome details of any service companies in Mexico. Additionally, who sells
consumables within Mexico, or is it best to purchase over the border in the USA.

Thanking you in advance.

Paul Rennie

Kidde International Research
Mathisen Way
Colnbrook
Slough
Berks SL3 0HB
UK

Tel: 44 (0) 1753 683245
Fax: 44 (0) 1753 683810
http://www.kidde-int.com



From daemon Thu Jan 06 18:00:52 2000



From: :      UICPRINT-at-uic.edu
Date: Thu, 06 Jan 00 08:41:26 CST
Subject: FREE SCREENINGS IN JANUARY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For Sale:

JEOL 100B - Disassembled, great source for Parts . $2,000 Cash and Carry.

PHILLIPS 201C - Will be taken out of service this month. Excellent working
condition. Maintained on OEM Service Contracts since day one. Make an offer.

--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
800-565-1895 phone
612-929-1895 fax
web site: http://www.aibltd.com




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Message-Id: {200001061637.KAA00322-at-Sparc5.Microscopy.Com}



The UIC Physicians Group at Central Station, 1550 South Indiana Avenue,
(312) 957-0049, is offering FREE SCREENINGS/TALKS in JANUARY:

"Stress Management"
Learn to manage the stress in your busy life.
All Fridays in January, 1:30-3:30 p.m.

"Bone Density Screening"
For the detection of osteoporosis
January 19--call for appointments

"Bowel and Bladder Management"
Tuesday, January 18, 9:30-11 a.m.
Friday, January 28, 9:30-11 a.m.

Special Feature
Are you overweight? Not sure? Find out your body mass index (BMI) and body
fat percentage. Also learn about UIC's new weight loss program and all
available options.
January 26, 9-11 a.m.




From: :      UICPRINT-at-uic.edu
Date: Thu, 06 Jan 00 08:41:26 CST
Subject: FREE SCREENINGS IN JANUARY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



From daemon Thu Jan 06 18:00:52 2000



From: Robert Plano :      RPLANO-at-cea.com
Date: Thu, 6 Jan 2000 08:47:59 -0800
Subject: RE: Looking for a Contract laboratory that does AFM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
id {CF52CAJP} ; Thu, 6 Jan 2000 08:48:07 -0800
Message-ID: {EAF569A980E4D21185380090271F40EB125F35-at-EXCHANGE}
{Microscopy-at-Sparc5.Microscopy.Com} ,
"',rfelten-at-Macdermid.com'"
{,rfelten-at-Macdermid.com}


Rick,

While we are not too close to Connecticut (California to be exact), we have
a great deal of experience in analyzing all types of samples with the AFM
and overnight delivery services can make turnaround times very short. What
information are you looking for from the samples? Please give me a call at
650-962-8767 or respond to this email so we can help you out.

-Rob

Robert J. Plano
Staff Analyst, SPM Services
Surface Science Labs
(650)962-8767, ext. 742
http://www.cea.com
http://www.surface-science.com


} -----Original Message-----
} From: "rfelten-at-Macdermid.com"-at-Sparc5.Microscopy.Com
} [mailto:"rfelten-at-Macdermid.com"-at-Sparc5.Microscopy.Com]
} Sent: Wednesday, January 05, 2000 1:17 PM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Looking for a Contract laboratory that does AFM
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
}
} Rick Felten-at-MACDERMID
} 01/05/2000 04:16 PM
} I have a couple of Ni/Au Samples that I need analyzed using AFM. The
} conductive samples would need to be scanned over a 10 X 10
} micron region.
} Were are located in Waterbury Ct and the closer to us the better.
}
} Any Help would be appreciated.
}
} Ric
}
}
}



From daemon Thu Jan 06 18:00:56 2000



From: rgriffin-at-eng.uab.edu
Date: Thu, 6 Jan 2000 12:11:25 -0600
Subject: 8 inch 10 megabyte bernoulli flexible disk cartridges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have about 45 USED 8 inch 10 megabyte bernoulli flexible disk cartridges
that I will ship to the first person that emails me at rgriffin-at-eng.uab.edu.
Due to shipping charges, I will only ship to a continental U.S. address.

We have gotten rid of our computer that uses these disks and I thought
someone out there that still needed them might appreciate them (since you
can't buy them anymore!).

Robin Griffin
Materials and Mechanical Engineering
The University of Alabama at Birmingham



From daemon Thu Jan 06 18:00:57 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Thu, 06 Jan 2000 12:35:35 -0600
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747




From daemon Thu Jan 06 18:00:59 2000



From: Barbara Foster :      mme-at-map.com
Date: Thu, 06 Jan 2000 15:24:14 -0500
Subject: Re: SEM- service companies in Mexico

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

Contact Gordon Grau Scientific (407-282-5749)

While they are in Florida, they deal extensively with Latin America. As
for either Dr. Neddy Fookes or Dr. Barry Fookes... tell them I sent you.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.


At 03:54 PM 1/6/00 +0000, Paul Rennie (KIDDE) wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jan 06 18:01:01 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 06 Jan 2000 16:11:22 -0500
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Osmium vapors can penetrate and blacken plastics. The long term effects
are uncertain. If you can find a Teflon bottle this might work.

Probably best to stay with glass bottles with Teflon colsures and do not
hand carry the solution (i.e. use a lab cart for transport). Hope this
is some help.

Charles Duvic, Ph.D.
Chief Chemist

--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Thu Jan 06 18:01:02 2000



From: jjerome-at-wfubmc.edu (Jay Jerome)
Date: Thu, 06 Jan 2000 16:38:45 -0500
Subject: Survey of Graduate Students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This was sent to the MSA Education Committee. It seems to be a worthwhile
endeavor, so I am passing it along to those on the listserver.
Jay Jerome

Adam Fagen wrote:

} Dear Microscopy Community:
}
} The National Association of Graduate-Professional Students (NAGPS) has
} recently received a grant from the Alfred P. Sloan Foundation to conduct a
} survey of doctoral students on their graduate school experiences. The
} survey will be completed on the Web {http://survey.nagps.org/} by current
} and recent doctoral students from January - May 2000, and the results made
} publicly available on the Web on a department specific basis in September.
}
} This effort is a follow-up to a more limited survey which occurred this
} past spring, which was aimed at science and engineering doctoral students.
} The aggregate results from that survey are available at
} {http://www.phds.org/survey/results/} .
}
} The survey we are conducting is unique in at least two important ways: it
} collects information on a department-specific basis, not only averaged
} over entire institutions or disciplines (though discipline-level results
} will also be available). So it will be possible to look at, for instance,
} responses from individual biology programs, or to rank history departments
} based on faculty mentoring. And it makes this data publicly available on
} the Internet in Fall 2000. So we'll be opening the door about the
} situation in individual departments for wider viewing by graduate
} students, prospective students, faculty, administrators, etc.
}
} The survey is based upon best practices and covers issues in a number of
} areas, including information for prospective students, curriculum breadth
} and flexibility, career guidance and placement services, faculty
} mentoring, time to degree, department climate, teaching, professionalism,
} and overall satisfaction. In other words, the sort of best practices and
} concerns outside of the reputation. The NAGPS survey itself will run from
} January 18-May 1, 2000, and will be available on the Web at
} {http://survey.nagps.org/} (which already has a number of resources).
}
} For this survey to be useful, it is vital that we reach as many current
} and recent doctoral students (anyone who has been enrolled for at least
} one semester in the past five years) as possible. We are hoping that we
} can encourage a significant percentage of students to respond so that the
} results will represent a broad range of experiences and a realistic
} picture of department and institutional practices.
}
} In order to realize this level of participation, getting the word out is
} obviously very important. One of the publicity strategies we're employing
} is trying to reach current and recent doctoral students through the major
} professional societies and organizations, such as the MSA. (Other
} strategies include messages to relevant e-mail listservs, coverage in
} campus and national media, and working through graduate student
} organizations, department chairs and graduate deans, other organizations,
} etc.)
}
} Since the MSA reaches so many current and recent graduate students, it is
} certainly one of the organizations of prime importance for getting the
} word out. We have thought of a few strategies for spreading the word
} among your membership (and would welcome additional ideas and
} suggestions): (1) distribution on e-mail listservs that reach a high
} number of graduate students and recent graduates, those who have left
} their programs, etc.; (2) notice in newsletters and other publications
} that current and former students might see; and (3) publicity at meetings
} and conferences. We are happy to provide whatever resources and materials
} that would facilitate distribution (e.g., flyers, letters, posters, etc.).
} We would certainly appreciate any insight you have on publicity within
} (and outside of) the MSA.
}
} As a bit of background on our organization, NAGPS represents nearly
} 900,000 graduate and professional students on 150 member campuses and is
} dedicated to improving the quality of graduate and professional student
} life and education by actively promoting the interests and welfare of
} graduate- and professional-degree-seeking students.
}
} Of course, I'd be happy to answer any questions or provide any more info.
} Thanks for your assistance in this important effort!
}
} --Adam Fagen
}
} Adam Fagen, Chair
} Ad Hoc Committee on Faculty-Student Relations
} National Association of Graduate-Professional Students (NAGPS)
}
} NAGPS Web: http://www.nagps.org/
} The National Doctoral Program Survey: http://survey.nagps.org/
}
} Adam Fagen \ afagen-at-fas.harvard.edu
} Molecular Biology/Education / http://www.fas.harvard.edu/~afagen/
} Harvard University GSAS \ http://mazur-www.harvard.edu/

--
Jay
----------------------------------------------
- AKA: W. Gray Jerome, Ph.D. -
- Department of Pathology -
- Wake Forest University School of Medicine -
- Winston-Salem, NC 27157-1092 -
- Ph: 336-716-4972, 336-716-2675 -
- Fax: 336-716-6174 -
- E-mail: jjerome-at-wfubmc.edu -
----------------------------------------------





From daemon Fri Jan 07 07:08:42 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 06 Jan 2000 16:46:43 -0800
Subject: Image montage software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone recommend image montaging software? That is, software that
assists you in compositing several images into one larger image. I am
familiar with the offerings from GATAN for their Digital Micrograph and
QuickStitch from Enroute Software. Also, can anyone offer an evaluation of
the QuickStitch software?

TIA


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Fri Jan 07 07:08:49 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 7 Jan 2000 15:11:32 +1000
Subject: RE: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Plastic is oxidized and so part of the osmium is exhausted in the vials. Even
when frozen osmium diffuses through plastic containers. So you may save a rare
breakage, but you are certain to have osmium in your refrigerator. It's just a
bad idea to pack osmium in plastic. You could use a secondary container, be it
plastic or glass, to increase overall safety.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 06, 2000 3:08 AM, Donna Wagahoff
[SMTP:DWAGAHOFF-at-siumed.edu] wrote:
}
} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227




From daemon Fri Jan 07 07:08:49 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 7 Jan 2000 10:24:22 +0000
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would not recommend storing osmium in ordinary laboratory
plastics containers used on their own. Osmium penetrates
polyolefin plastics (PE, PP) to some depth, and reacts with them,
so containers made from these plastics or of polystyrene or
polycarbonate with polyolefin closures cannot be recommended.
Mostly, osmium is supplied in some protective packaging, but if
you want to improve security still further I would consider placing
the glass ampoules inside a polyethylene or polypropylene tube.
That would give considerable shock-protection, and if the ampoule
did break would protect against leakage, but only for the short
period required to get it to a fume cupboard. The same principle
can be applied to osmium solutions prepared in glass bottles -
enclosure in an outer polyethylene bottle will give shock-protection
and temporary containment. It will also indicate by its blackening
how much leakage is occurring from your supposedly closed glass
container. Speaking of which, we have a rule that osmium solutions
are NEVER stored in the laboratory refrigerator. Why? because
even when greatest care is taken, osmium blackening of the fridge
walls and other objects in the fridge takes place, indicating that
vapour is escaping into the fridge atmosphere. If you must store
osmium at low temperatures, I recommend you consider installing
a compact refrigerator in your fume hood or in a fan-ventilated
cupboard.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227
}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Fri Jan 07 07:38:57 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Fri, 07 Jan 2000 14:16:32 +0100
Subject: Mat: Cutting of small semiconductor specimen?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A happy New Year to everybody!

I just solved (with the help of some of you) my last problem with the
carbon extraction replicas but the next specimen causing difficulties is on
my desk...

I got a small semiconducor specimen with a layered structure that should be
investigated. If I say small I mean it: it has a shape like a cube with a
side length of about 250um. And now comes the real difficulty: We need to
cut this one precious specimen into several slices in order to allow other
investigations with other analytical methods. In fact we have five
specimens to try with, but they are not completely identical to the one we
have finally to investigate.

We are equipped with everything we need to do a TEM preparation of bulk
materials and also of interfaces as long as the specimens are large enough
(saw, disk cutter, dimpler, ion etcher, mounting tools, ...) but I don't
see how I could be successful with such a small specimen.

Any ideas?

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Fri Jan 07 07:58:55 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Fri, 07 Jan 2000 14:16:32 +0100
Subject: Mat: Cutting of small semiconductor specimen?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A happy New Year to everybody!

I just solved (with the help of some of you) my last problem with the
carbon extraction replicas but the next specimen causing difficulties is on
my desk...

I got a small semiconducor specimen with a layered structure that should be
investigated. If I say small I mean it: it has a shape like a cube with a
side length of about 250um. And now comes the real difficulty: We need to
cut this one precious specimen into several slices in order to allow other
investigations with other analytical methods. In fact we have five
specimens to try with, but they are not completely identical to the one we
have finally to investigate.

We are equipped with everything we need to do a TEM preparation of bulk
materials and also of interfaces as long as the specimens are large enough
(saw, disk cutter, dimpler, ion etcher, mounting tools, ...) but I don't
see how I could be successful with such a small specimen.

Any ideas?

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Fri Jan 07 18:08:50 2000



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Fri, 07 Jan 2000 09:40:08 -0500
Subject: Re: Mat: Cadmium Zinc Telluride thin foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dorrance,

I think I can make several suggestions and point you toward several references
that may help.

First, can you or have you tried dipping your ion milled (to perforation)
samples into your bromine/methanol etch as the final step in your procedure?
Yu et al (J.of Elec. Micro.Tech./18/315-324/1991) dipped their perforated
samples in 0.5% Br2/Ch3OH for 1or 2 seconds followed by 20 seconds in HF/HCL of
volume ratio 1:1. This technique worked well for them.

As you may know, reducing the energy during the final step(s) of ion milling
can reduce the severity and/or the number of artifacts produced. If you have
access to a mill that allows milling in the 100eV to 1keV range you may want to
try this as the final step in your milling procedure.

The following techniques have also been used to prepare type II-VI compound
semiconductors:

(1) Reactive ion milling. This can be done 2 different ways. In one
method
Iodine gas is used instead of Argon. The iodine gas is ionized to form I+,
which is then used for milling (Cullis and Chew/ MRS symposium proceedings/
115/ 3-14/1988). In the second method, Iodine gas is introduced into the
“Atmosphere” of the milling chamber during Ar+ milling (Pecz and Barna
/Vacuum/45/1-3/1994). The authors have applied these techniques to type II-VI
compound semiconductors of CdTe, ZnS, and ZnSe in addition to other compound
semiconductors. CAUTION must be used when introducing iodine gas into your ion
mill. The gas is very corrosive and may damage mill parts and vacuum systems.
I recommend that you check with the manufacturer of your ion mill before
proceeding.
(2) Chemomechanical polishing. Sabinina and Gutakovsky
(Ultramicroscopy/45/411-415/1992) eliminated the need for ion milling
completely by using this technique. They prepare samples of HgCdTe using a
bromine-methanol etchant in a technique that is very similar to dimpling using
padded tools.


Are you familiar with the Small Angle Cleavage Technique (SACT)? This
technique may or may not work for your materials. I am not aware of any
references for preparing II-VI materials using SACT but that certainly does not
imply that it can’t be done. I have used this technique to prepare samples of
thin films on silicon substrates. It is quick and relatively easy to learn.
The technique was developed by John McCaffrey and a good step by step procedure
is given by Walck and McCaffrey (MRS symposium proceedings/480/149-171/1997).

The references listed above are meant to be a starting point and are by no
means a complete listing.

Hope this helps.

E. Windsor

Eric Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
fax: (301) 417-1321
eric.windsor-at-nist.gov

Dorrance McClean originally wrote:

Hi,
I've been working on thin foils of CZT and I'm looking for some suggestions
to help eliminate artifacts created by ion milling. So far I have polished
side one and etched (to remove polishing damage) with bromine-methanol and
then dimpled side two, ending with 0.1um alumina. I have been successful
obtaining a final sample thickness of about 8 to 10 microns. However, when
I put the samples into the PIPs (Gatan) to complete the thinning process I'm
seeing beam damage. I was hoping that someone might have a different
approach or suggestion that would eliminate the beam damage.
Thanks for your help.
Dorrance




From daemon Fri Jan 07 18:08:48 2000



From: David Rohde :      drohde-at-zuul.noran.com
Date: Fri, 7 Jan 2000 08:41:45 -0600
Subject: Image montage software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soft Imaging Systems has a program called analySIS that has a montage module
called MIA. Have a look at their web site at: www.soft-imaging.de

David Rohde
NORAN Instruments

-----Original Message-----
} From: Rick Harris [mailto:raharris-at-ucdavis.edu]
Sent: Thursday, January 06, 2000 6:47 PM
To: microscopy-at-sparc5.microscopy.com


Can anyone recommend image montaging software? That is, software that
assists you in compositing several images into one larger image. I am
familiar with the offerings from GATAN for their Digital Micrograph and
QuickStitch from Enroute Software. Also, can anyone offer an evaluation of
the QuickStitch software?

TIA


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu





From daemon Fri Jan 07 18:08:49 2000



From: mcannon-at-bio.umass.edu (Maura Cannon)
Date: Fri, 7 Jan 2000 09:53:47 -0500
Subject: in situ hyb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Subscribers,
I would be interested in hearing from anyone who has experience in in situ
hybridization to mRNA. I am working with immature embryos of Arabidopsis
and would like to recieve replies and comments to the following questions:

1. For embedding, Paraffin is mostly used.
Why not use LR-White (London Resin)?
or BMM (Butylmethylmethacrylate)?
or another acrylic material?
These hydrophilic materials should be easier to remove, or is it necessary
to remove them for mRNA exposure?
If it is necessary, what is best for removing them? Perhaps acetone?

2. About the probe itself, obviously RNA is best. Has anyone tried DNA
oligos (~20 nucleotides) for less abundant mRNAs?

3. Has anyone used tailed oligos?

4. Is DIG significantly better to use than biotin labelled probes?

Many thanks to anyone who is prepared to take the time to help reveal the
unknowns of plant embryogenesis.
Maura
____________________
Dr. Maura C. Cannon
Dept. Biochemistry & Molecular Biology
Lederle Graduate Research Center
University of Massachusetts
Amherst, MA 01003






From daemon Fri Jan 07 18:08:49 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 7 Jan 2000 09:54:45 -0500
Subject: RE: Cutting of small semiconductor specimen?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron Anderson has a technique that might do the trick for you. He took an
ultrasonic drill and made a small cavity in a piece of Si at the surface.
The drill was solid spherical end. He then epoxied his small sample into
the hole. You might want to mount it on another piece of Si first and then
put it in the hole. He then used the Tripod Polishing technique to examine
it. The benefit is that he has the Si to gauge the thickness of the sample.
I though that it was pretty slick.

Your other option and the one most easy to do if you have access to an
instrument is to have the sample FIB'd.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--


} -----Original Message-----
} From: Petra Wahlbring [mailto:wahlbrin-at-crpgl.lu]
} Sent: Friday, January 07, 2000 8:17 AM
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Mat: Cutting of small semiconductor specimen?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} A happy New Year to everybody!
}
} I just solved (with the help of some of you) my last problem with the
} carbon extraction replicas but the next specimen causing
} difficulties is on
} my desk...
}
} I got a small semiconducor specimen with a layered structure
} that should be
} investigated. If I say small I mean it: it has a shape like a
} cube with a
} side length of about 250um. And now comes the real
} difficulty: We need to
} cut this one precious specimen into several slices in order
} to allow other
} investigations with other analytical methods. In fact we have five
} specimens to try with, but they are not completely identical
} to the one we
} have finally to investigate.
}
} We are equipped with everything we need to do a TEM
} preparation of bulk
} materials and also of interfaces as long as the specimens are
} large enough
} (saw, disk cutter, dimpler, ion etcher, mounting tools, ...)
} but I don't
} see how I could be successful with such a small specimen.
}
} Any ideas?
}
} Petra
}
}
} --------------------------------------------------------------
} Dr. Petra Wahlbring
} Centre de Recherche Public - Gabriel Lippmann
} Laboratoire d'Analyse des Materiaux (LAM)
} 162a, av. de la Faiencerie L-1511 Luxembourg
} tel. +352-466644-402 fax +352-466644-400
} e-mail: petra.wahlbring-at-crpgl.lu
} Visit our WWW site! http://www.crpgl.lu/~wahlbrin
}



From daemon Fri Jan 07 18:08:53 2000



From: rlvaughn-at-unmc.edu
Date: Fri, 7 Jan 2000 09:30:03 -0600
Subject: Re: Osmium in plastic bottle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Every plastic container we have seen turns black and some seals break down.
Our current method is similar to Bill Trivol's. We use a 25ml Pyrex media
bottle with the orange plastic lid (it does not seem affected). This is
placed in a spare metal can (that the ampules of osmium crystals were
shipped in) with paper padding. This new bottle (with parafilm around
cap) also solved our osmium fumes in the refrig. problem. Everything is
small enoug to transport from refrig. to hood.

Rick Vaughn




From daemon Fri Jan 07 18:08:52 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Fri, 7 Jan 2000 11:22:11 -0500
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ON 1/7/00 DR CHRIS JEFFREE WROTE:

Speaking of which, we have a rule that osmium solutions
are NEVER stored in the laboratory refrigerator. Why? because
even when greatest care is taken, osmium blackening of the fridge
walls and other objects in the fridge takes place, indicating that
vapour is escaping into the fridge atmosphere. If you must store
osmium at low temperatures, I recommend you consider installing
a compact refrigerator in your fume hood or in a fan-ventilated
cupboard.

With all due respect Chris for over 20 years we have used our lab
refrigerator to store Osmium with no blackening of the plastic walls. A
25ml 4% solution of Osmium is put into a 60 ml glass stopper bottle with
the stopper tightly wrapped in parafilm. Next, that bottle is placed in a
waxed 6" long x 3" diameter screw capped cardboard mailing tube. We prepare
the waxed tubes by pouring hot paraffin inside and rotated quickly and
evenly until the entire surface area is well coated. We also coat the
inside of the metal screw cap but not the outside of the tube. Mailing
tubes are available from the local shipping supply house and our waxed
containers have lasted decades.
Regards
Jim

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax





From daemon Fri Jan 07 18:08:53 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 07 Jan 2000 12:48:42 -0500
Subject: Re: Osmium in plastic bottle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been using teflon bottles for many yeas with success. We
got them from Fisher Sci

At 09:30 AM 1/7/00 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Fri Jan 07 18:08:57 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 07 Jan 2000 11:57:20 -0800
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donna Wagahoff wrote:

} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.

Dear Donna,

In my point of view polypropylene and polyethylene are compatible with
osmium tetroxide solutions. Only problem - to hold that nasty stuff inside
the vial. Some plastics may partially be penetrable by osmium tetroxide.

I had some limited experience to store aqueous osmium tetroxide solutions
in the polypropylene (?) NUNC 2 or 5 ml cryo-vials. I aliquot solution
into that vials and store it at -40oC. For extra protection I stored
cryo-vials in the 15 ml cell-culture tubes. Cryo-vial's seal may penetrate
by osmium tetroxide vapors a little bit but second tube provides complete
protection. 2-4% aqueous osmium tetroxide solutions (no buffer, please)
are stable for at least 1-2 month in the dark at -20 - 40oC in that plastic
containers.

Good luck.

Sergey.

_________________________________

Sergey Ryazantsev Ph. D.
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Fri Jan 07 18:08:57 2000



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Fri, 07 Jan 2000 14:21:38 -0600
Subject: SEM of biofilms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

There is a researcher at our Med. School who wants to do SEM on biofilms

on ear biopsies. He says the biopsies will be approximately 1mmX1mm. I
proposed a standard glut.-osmium fix, Et-oh dehydration, CPD, mount,
sput coat protocol. Is this sufficient? Do we need to affix the biopsies

to a substrate first? I would appreciate any suggestions. If anybody has
reprints that contain a good protocol, I would certainly appreciate
getting a copy. My address is in the footer and my FAX is 405-325-7619.
Thanks for your help.

Bill Chissoe


--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================





From daemon Fri Jan 07 18:09:01 2000



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Fri, 7 Jan 2000 15:55:56 -0700
Subject: Vac Evap: Special needs request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A user of our facility wishes to do some special vacuum evaporation
processes. He is looking for ceramic coated tungsten boats for the
purpose, but has not been able to locate a source. Any help would be
appreciated.

Replies offline would be welcome. Please reply to the list only if you
have generally useful information.

Thanks,

John Chandler
Colorado State University
chandler-at-lamar.colostate.edu





From daemon Fri Jan 07 18:09:01 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 7 Jan 2000 17:23:00 -0600
Subject: BSE imaging artifact - Help!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

For years, I have been using my diode GW BSE system with high beam currents
and
high gain (contrast) to yield electron channeling contrast on polished
specimens.

FWIW: Contrary to some reports I have read, lower kV works better than
higher.
Since it is imperative to use high beam currents at the required resolution,
I
wonder if difficulty in achieving sufficient current at lower potentials may
have been influencing opinions.

I have also been a very good customer for GW Electronics. When the detector
is
new, I generally have no problem, but as it ages, the same operating
conditions
will produce artifacts. A new detector every year or so is a bit expensive,
but
fixes the problem.

I now have a new detector (and SEM :) which produces this artifact and am
now
trying to find the reason.

The artifact can be described as video brightness overshoot from black to
white
when the black is strongly saturated. It occurs when the (very high gain)
BSE
signal goes from saturated black to some median gray. A low-Z inclusion or
deep
pore in the field of view will produce this artifact. When the beam leaves
the
inclusion/pore, it overshoots to white then settles back to the appropriate
level. The effect is a black pore with a white "comet tail" pointing in the
sweep direction. This problem does not manifest itself when using less
extreme
operating conditions.

Any suggestions about how to avoid the artifact?

TIA for your comments and help.

Woody White
McDermott Technology, Inc.



From daemon Fri Jan 07 18:09:01 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 7 Jan 2000 17:41:00 -0600
Subject: More on BSE artifact

Contents Retrieved from Microscopy Listserver Archives
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PS...

I certainly cannot exclude the possibility that the detector is not the
direct
cause. The amount of signal gain (contrast) is not calibrated (nor have I
paid
much attention to the position of the adjustment) . If the detector simply
loses some sensitivity, I would make up the difference in amplifier gain and
not
realize I was not at exactly the same operating conditions. ...Thus, the
artifact could be amplifier gain related rather than simply a detector
problem.

Woody



From daemon Sat Jan 08 07:54:27 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 08 Jan 2000 00:16:19 -0500
Subject: Shipping to Mexico

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Paul Rennie wrote:
===============================================
Having just purchased a new SEM, our old instrument, a Cambridge S200 is
destined to be sent to one of our companies in Cuidad Victoria, Mexico
(Although I'm trying to persuade management that it's not worth it).
Although we will probably be installing the instrument ourselves, I would
welcome details of any service companies in Mexico. Additionally, who sells
consumables within Mexico, or is it best to purchase over the border in the
USA.
================================================
With regard to the method of purchasing consumables from within Mexico, the
situation is entirely analogous to that that exists in the UK vis a vis
purchasing items from the USA directly or via a distributor in the UK. It
is a matter of institutional policy and also, personal preference.

The main manufacturers of consumables all have local distributors in Mexico
and those firms can be found on the websites of those respective firms. On
the other hand, with the implimentation of NAFTA, making a shipment to a
point in Mexico from the USA is not really all that different from making a
shipment to some other point in the USA. So again it is a matter of
institutional policy and personal preference.

Chuck


============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Sat Jan 08 12:07:30 2000



From: Lugosi1936-at-aol.com
Date: Sat, 8 Jan 2000 12:41:36 EST
Subject: LM Vickers Microscope M1500974C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have been trying for some time to find any information about a Vickers
Instrument Company microscope. I had never heard of the Company but the scope
looked interesting. I have searched hundreds of websites, posted messages on
newsgroups and also on Microscopy, UK. So far I've had only two responses.
The sum total of what I have learned is that the company was in York and that
they closed in 1989. Cooke, Troughton and Simms were taken over by Vickers

The rather lengthy description of the scope follows:

Head: Binocular configuration, adjustable for interpupillary distance and
dioptric differences. Interpupillary adjustment range, 50 to 72mm.
Eyepieces(2): 10X, DIN standard. Objectives(4): Vickers microplan,
Planachromatic objectives. Include 5X (0.15 N.A.), 10X (0.25 N.A.), 20X (0.50
N.A. spring loaded), 40X (0.65 N.A. spring-loaded), DIN standard objectives.
All are parfocal, parcentered, and coated to resist reflection. Stage:
Precision-machined mechanical stage with oversized, low-position, coaxial
control knobs. Chemical-resistant finish with glass insert. This stage is
exceptionally smooth in movement with a 3.25 x 2.75-inch range of motion.
Focus Control: Low-position, coaxial, coarse/fine controls. . Condenser: Abbe
condenser, fitted with an iris diaphragm. Illumination: Diascopic lower
illumination features variable-intensity quartz halogen lamp ( 12V, 100 watt
) with metered solid-state control as well as field iris, condenser and
centering adjustments. Episcopic illumination (30-watt lamp) is also of
variable intensity via an independent control on front of the microscope
base. Upper illuminator housing fitted with iris and condenser controls. As
shown in the photos, the microscope can be separated from the 100-watt
illuminator base Finish chemical-resistant paint with "hammertone" finish.

I have included some pictures that I have posted to help with the
identification:

{A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l
ugosi1936/vic1a.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu
gosi1936/vic2.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu
gosi1936/vic3.jpg {/A}

I am trying to find any information about the company and about the scope
itself. I understand that this is rather difficult (impossible?) because of
the practice of the company not to use serial numbers. I would especially
like to be able to aquire a copy of the owners manual and a copy of the
Vickers catalog.

When I began to attempt to collect information about the Vickers I never
guessed that I would run into a blank wall. If you could assist me in any way
at all it would be greatly appreciated.

Best regards,
Dana



From daemon Sun Jan 09 10:49:57 2000



From: Melany H. Chapin :      mchapin-at-ntbg.org
Date: Sat, 8 Jan 2000 13:42:27 -1000
Subject: Histochemical test for lipids/oils in plant tissue

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a simple method to indicate the presence of lipids /oils
in plant tissue. Specifically palm fruit tissues. Ideally, if there is a
procedure that I can used on fruits fixed in FAA that would be the best. I
have tried Sudan Black B without success. Thank you in advance for your
ideas or suggestions. Please reply via email to : mchapin-at-ntbg.org

________________________________________________________________________

Melany H. Chapin Herbarium (PTBG)
Curator & Plant Records Manager ph: 808-332-7324 ext. 133
National Tropical Botanical Garden (NTBG) fax: 808-332-9765

3530 Papalina Road email: mchapin-at-ntbg.org
Kalaheo, Kauai, Hawaii 96741 www.ntbg.org
USA
___________________________________________________________________________





From daemon Sun Jan 09 10:50:14 2000



From: Campbell36-at-aol.com
Date: Sun, 9 Jan 2000 10:45:02 EST
Subject: Re: Vac Evap: Special needs request

Contents Retrieved from Microscopy Listserver Archives
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The item you are looking for should be available from R.D. Matis. The
following contact information is from www.vacuum.org. There are several
vendor listed in the subsection for filaments if you care to browse. I have
no financial interest in R.D. Matis.

R.D. Mathis Company
2840 Gundry Avenue Long Beach, CA 90806
Phone: 562-426-7049
FAX: 562-595-0907

Description

Specializes in the manufacture of hi-vacuum evaporation sources. We offer a
comprehensive selection of tungsten, molybdenum and tantalum sources as well
as custom fabrication to meet your specific needs. Display will be a variety
of evaporation sources along with one of our "LV Series" low voltage high
current power supplies and our "GP 100" inert gas purifier to compliment your
evaporation process.

Product Categories
Filaments



Good luck

Jim Campbell

James Campbell
36 Van Drive
Bordentown, NJ 08505
Tel: 609-298-9206
Fax: 609-278-6969
e-mail campbell36-at-aol.com



In a message dated 1/7/00 9:56:09 PM Eastern Standard Time,
chandler-at-lamar.ColoState.EDU writes:

} Subj: Vac Evap: Special needs request
} Date: 1/7/00 9:56:09 PM Eastern Standard Time
} From: chandler-at-lamar.ColoState.EDU (John Chandler)
} To: Microscopy-at-Sparc5.Microscopy.Com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} A user of our facility wishes to do some special vacuum evaporation
} processes. He is looking for ceramic coated tungsten boats for the
} purpose, but has not been able to locate a source. Any help would be
} appreciated.
}
} Replies offline would be welcome. Please reply to the list only if you
} have generally useful information.
}
} Thanks,
}
} John Chandler
} Colorado State University
} chandler-at-lamar.colostate.edu



James Campbell
36 Van Drive
Bordentown, NJ 08505
Tel: 609-298-9206
Fax: 609-278-6969
e-mail campbell36-at-aol.com



From daemon Sun Jan 09 19:10:36 2000



From: Michael L. Boucher :      mboucher-at-isd.net
Date: Sun, 9 Jan 2000 13:58:37 -0600
Subject: Vickers

Contents Retrieved from Microscopy Listserver Archives
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Dana:
This may not help, but I have a book I purchased about 1970 that was
published by Vickers entitled:
The Polarizing Microscope by A.F. Hallimond.
It is an excellent work and has many pictures of the Vickers line of
polarizing scopes and accessories from the late 60s. They did buy out Cooke,
Troughton and Simms and marketed a number of innovative and competitively
priced (cheaper than Leitz and Zeiss) scopes. Dr Hallimond was one of their
consultants on design. I am sure you can find the book in a large University
Geology department library ( I hope). Or try interlibrary loan. It is still
an excellent and definitive reference for polarizing light microscopes and
their use.

Michael L. Boucher Sr.
mboucher-at-isd.net
http://www.isd.net/mboucher




From daemon Sun Jan 09 19:10:39 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 09 Jan 2000 15:50:09 -0800
Subject: Low Z contrast quantitative analysis

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I am trying to quantify the alloy amount of Al/Si. The standard
alloy ratio is 1-3 wt % Si/Al. However, with Z of each only 1 unit
apart, and low Z at that, EDAX and AES cannot detect the Si.
My next attempt is to try dynamic SIMS and then time of flight SIMS.

The specimen is a microcircuit die. I am analyzing the bonding pad
metalization.

Has anyone done this sort of thing before and had success? If so,
how did you do it?

gary g.




From daemon Sun Jan 09 19:10:39 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 09 Jan 2000 18:53:50 -0500
Subject: Re: LM Vickers Microscope M1500974C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dana,

Vickers was a well known British manufacturer of microscopes. I have had
the privilege of using some of their more interesting measuring equipment.
Several contacts come to mind, most of them from the UK: Clive Cowan, Micro
Instruments in Oxford (UK) 199-388-9616 and Gerard Turner, who is a curator
in microscopy at the Museum of Science at Oxford University, Dr. Savile
Bradbury, retired from Oxford (but could probably be reached by letter
there; also, if you are interested, I can probably get a more recent
address), and Dr. Cecile Fox at Molecular Histology in Gaithersburg
(301-216-1564). Cecile put together major microscopy exhibits for both the
Smithsonian and the Am. Mus. of Natural History in the mid- 1980's. Please
give my regards to them (Gerard may only remember me as an RMS student from
the distant past) and best of luck on your search.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.




At 12:41 PM 1/8/00 EST, Lugosi1936-at-aol.com"-at-Sparc5.Microscopy.Com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 10 08:06:03 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 10 Jan 2000 13:39:27 +1000
Subject: RE: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
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Do you have an evaporator in the lab? Simultaneous evaporation of Pt (wire) and
carbon gives a very fine and permanent coating.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 06, 2000 2:15 AM, Tindall, Randy D.
[SMTP:TindallR-at-missouri.edu] wrote:
}
}
} Hi,
}
} Due to some recent high-resolution requirements in our lab, I find myself
} having to go back to Sputter Coating 101 (after years of just putting
} specimens in the coater and turning it on without a second thought!). We
} find ourselves in need of very thin coatings with as little structure as
} possible, in order to image samples down in the nanometer range.
}
} We have a chromium coater, but often need to revisit samples days or weeks
} after the initial coating. The oxidation problem rears its ugly head. We
} intend to purchase a platinum target, but don't yet have one, so we're
} experimenting with our venerable Au/Pd coater.
}
} My questions are:
}
} 1) I seem to remember a string on this listserver suggesting that lower
} deposition currents yield finer coating structure. Is this right? Does a
} low deposition current for a longer time yield a finer coating than a higher
} current for a shorter time? (I'm running some tests to check this, but
} would be very interested in others' experiences, too.)
}
} 2) Deposition current can be controlled by the initial current setting
} (i.e., the knob on the machine) and by the argon flow through the chamber.
} Is there any difference in the coating when adjusting the deposition current
} by either of these two ways?
}
} 3) Charts I have seen indicate that deposition current is directly
} proportional to coating rate. Is the same true for coating time? I.e., is a
} one minute coating twice as thick as a 30 sec. coating? It would seem so
} intuitively, but you know what they say about the word "assume".
}
} My apologies if these are very basic questions, but, like I said, back to SC
} 101!
}
} I'll be happy to summarize the responses for anyone who is interested.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine Bldg.
} University of Missouri
} Columbia, MO 65211
} (573)882-8304
} tindallr-at-missouri.edu
} http://www.biotech.missouri.edu/emc/
}




From daemon Mon Jan 10 17:49:35 2000



From: rschoonh-at-sph.unc.edu
Date: Mon, 10 Jan 2000 09:54:45 -0500 (Eastern Standard Time)
Subject: Re: LM Vickers Microscope M1500974C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dana,

I'm not 100% sure but I thought that the Microscience Division of Bio-Rad bought
Vickers some years ago. You might want to give them a shot.

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

-- Begin original message --
} -----------------------------------------------------------------------.
}
}
}
} I have been trying for some time to find any information about a Vickers
} Instrument Company microscope. I had never heard of the Company but the scope
} looked interesting. I have searched hundreds of websites, posted messages on
} newsgroups and also on Microscopy, UK. So far I've had only two responses.
} The sum total of what I have learned is that the company was in York and that
} they closed in 1989. Cooke, Troughton and Simms were taken over by Vickers
}
} The rather lengthy description of the scope follows:
}
} Head: Binocular configuration, adjustable for interpupillary distance and
} dioptric differences. Interpupillary adjustment range, 50 to 72mm.
} Eyepieces(2): 10X, DIN standard. Objectives(4): Vickers microplan,
} Planachromatic objectives. Include 5X (0.15 N.A.), 10X (0.25 N.A.), 20X (0.50
} N.A. spring loaded), 40X (0.65 N.A. spring-loaded), DIN standard objectives.
} All are parfocal, parcentered, and coated to resist reflection. Stage:
} Precision-machined mechanical stage with oversized, low-position, coaxial
} control knobs. Chemical-resistant finish with glass insert. This stage is
} exceptionally smooth in movement with a 3.25 x 2.75-inch range of motion.
} Focus Control: Low-position, coaxial, coarse/fine controls. . Condenser: Abbe
} condenser, fitted with an iris diaphragm. Illumination: Diascopic lower
} illumination features variable-intensity quartz halogen lamp ( 12V, 100 watt
} ) with metered solid-state control as well as field iris, condenser and
} centering adjustments. Episcopic illumination (30-watt lamp) is also of
} variable intensity via an independent control on front of the microscope
} base. Upper illuminator housing fitted with iris and condenser controls. As
} shown in the photos, the microscope can be separated from the 100-watt
} illuminator base Finish chemical-resistant paint with "hammertone" finish.
}
} I have included some pictures that I have posted to help with the
} identification:
}
} {A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l
} ugosi1936/vic1a.jpg {/A}
} {A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu
} gosi1936/vic2.jpg {/A}
} {A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu
} gosi1936/vic3.jpg {/A}
}
} I am trying to find any information about the company and about the scope
} itself. I understand that this is rather difficult (impossible?) because of
} the practice of the company not to use serial numbers. I would especially
} like to be able to aquire a copy of the owners manual and a copy of the
} Vickers catalog.
}
} When I began to attempt to collect information about the Vickers I never
} guessed that I would run into a blank wall. If you could assist me in any way
} at all it would be greatly appreciated.
}
} Best regards,
} Dana
}
}

-- End original message --




From daemon Mon Jan 10 17:49:37 2000



From: Michael Reiner :      michael.reiner-at-Smail.Uni-Koeln.de
Date: Mon, 10 Jan 2000 16:23:07 +0100
Subject: EM: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
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Dear members of the list,

first, I would like to wish you all the best for the new year.
Now my question:
Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?
Is it that delicate as LR-White (Meanwhile I don«t use LR-W older than
half a year). My bottle which was not opened many times, could be
roundabout three years old.

Thanks a lot,
Michael

Michael Reiner
Department of Anatomy I
University of Cologne
Germany
michael.reiner-at-smail.uni-koeln.de




From daemon Mon Jan 10 17:49:37 2000



From: carol williams :      cswill-at-acd.tusk.edu
Date: Mon, 10 Jan 2000 09:23:30 -0600
Subject: gamma correction - image analysis

Contents Retrieved from Microscopy Listserver Archives
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Please send me (if possible) a brief explanation of gamma correction in
image analysis and a good reference source (book, papers, etc) to get
the details... Thanks



From daemon Mon Jan 10 17:49:40 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 10 Jan 2000 08:48:07 -0800
Subject: Re: SEM: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
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Dear Randy,
There was a good series of articles in the Scanning Electron Microscopy,
1980, volume I (pp. 143--218), that examined a number of thin-film
deposition methods and measured the films for feature size, mostly using
TEM. They found that lower kV made for finer films, using Mo, W or Ta made a
more featureless coating, Pt was a little finer than Au/Pd and that lowering
the temperature of the substrate also made the films finer.
In my own experience I found that, on smoother specimens, even a two second
coating would sometimes be enough to stop charging. Try very short times and
turn the specimen and coat again for a very short time, if the first one
isn't enough. I used 700V, if your coater can change voltage.
If you can find the Scanning Electron Microscopy articles, they have other
good suggestions.
At 10:15 AM 1/5/00 -0600, you wrote:

} Hi,
}
} Due to some recent high-resolution requirements in our lab, I find myself
} having to go back to Sputter Coating 101 (after years of just putting
} specimens in the coater and turning it on without a second thought!). We
} find ourselves in need of very thin coatings with as little structure as
} possible, in order to image samples down in the nanometer range.
}
} We have a chromium coater, but often need to revisit samples days or weeks
} after the initial coating. The oxidation problem rears its ugly head. We
} intend to purchase a platinum target, but don't yet have one, so we're
} experimenting with our venerable Au/Pd coater.
}
} My questions are:
}
} 1) I seem to remember a string on this listserver suggesting that lower
} deposition currents yield finer coating structure. Is this right? Does a
} low deposition current for a longer time yield a finer coating than a higher
} current for a shorter time? (I'm running some tests to check this, but
} would be very interested in others' experiences, too.)
}
} 2) Deposition current can be controlled by the initial current setting
} (i.e., the knob on the machine) and by the argon flow through the chamber.
} Is there any difference in the coating when adjusting the deposition current
} by either of these two ways?
}
} 3) Charts I have seen indicate that deposition current is directly
} proportional to coating rate. Is the same true for coating time? I.e., is a
} one minute coating twice as thick as a 30 sec. coating? It would seem so
} intuitively, but you know what they say about the word "assume".
}
} My apologies if these are very basic questions, but, like I said, back to SC
} 101!
}
} I'll be happy to summarize the responses for anyone who is interested.
}
} Thanks!
}
} Randy
}
Best of luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Jan 10 17:49:43 2000



From: Joanne Crudele :      Joanne.Crudele-at-unilever.com
Date: Mon, 10 Jan 2000 12:01:46 -0600
Subject: CryoTEM or SEM

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Microscopy-at-MSA.Microscopy.Com

Is anyone willing to do some contract cryoSEM or TEM in the Chicagoland area? I
have a primary emulsion with particle size less than 1.0 micrometers. 2 samples.
Please contact me via the server. Joanne Crudele-Unilever-Rolling Meadows Il.
Give a price estimate per sample.



From daemon Mon Jan 10 17:49:42 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: 1/8/00 7:11 PM
Subject: Re: More on BSE artifact

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John,

You are corrrect in that the time constant of diode BSE detectors is
relatively
slow. Unfortunatly, I am already sweeping about as slowly as possible to
minimize the effect and help the signal to noise ratio.

Woody
____________________Reply Separator____________________

Woody,
Is the problem independent of scan speed? Slower scan speeds
often overcome distortion using solid state BEI detectors.


At 05:41 PM 1/7/00 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 10 08:06:03 2000



From: IKSM :      IKSM-at-aphy.iphy.ac.cn
Date: Mon, 10 Jan 2000 19:3:24 +0800
Subject: IKSM: latest news -- call for paper

Contents Retrieved from Microscopy Listserver Archives
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id AA30574; Mon, 10 Jan 2000 19:01:49 +0800
Message-Id: {200001101101.AA30574-at-aphy.iphy.ac.cn}


Hi, Microscopist,

International Kunming Symposium on Microscopy (IKSM)
will be held on July 2-5, 2000, in Kunming, one of the
most attractive tourist destinations in China.

Call-for-paper circular and pre-registration form for
International Kunming Symposium on Microscopy (IKSM)
are available on request by email. For more information,
pls visit http://www.iphy.ac.cn/microsc/IKSM.html .

Happy a new year 2000.

Regards,
IKSM secretariat
IKSM-at-aphy.iphy.ac.cn




From daemon Mon Jan 10 17:49:48 2000



From: rfelten-at-Macdermid.com
Date: Mon, 10 Jan 2000 16:20:40 -0500
Subject: Re: Image montage software

Contents Retrieved from Microscopy Listserver Archives
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Rick Felten-at-MACDERMID
01/10/2000 04:20 PM
Whenever I wanted to merge images I used corel draw 8. I inserted the
images where I wanted them and exported the entire image as a tiff. Seem
to work ok. Not sure if it is the most efficient way. In fac, it is the
only thing that I liked about corel draw over MS publisher.

Ric





From daemon Mon Jan 10 17:49:48 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 10 Jan 2000 16:43:55 -0500 (EST)
Subject: Spotty artifacts

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To the Board,
We have a problem with spotty,black,amorphous artifacts on our
SEM samples visible at 200X and up. The problem seems to arise only
with one sample type, epoxy resin casts made with amine-blush resistant
hardener. We use professional dental impression molds,a two part process, base
and catalyst to make the flexible mold (President, polyvinylsiloxane). Then
Araldite 506 epoxy with HY 356 hardener is poured and cured at room temp
overnight. Sputter coating glass slides with gold palladium does not
seem to cause the artifact, but another question, is gold palladium more
susceptible to oxide formation than pure gold, and do oxides look like
the above described artifact. Also, etching the sample does not help.
Any suggesstions will be greatly appreciated. Thanks.

Mike D.




From daemon Mon Jan 10 17:49:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 10 Jan 2000 18:13:14 -0500 (EST)
Subject: Re: Osmium in plastic bottle

Contents Retrieved from Microscopy Listserver Archives
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We store Os in a glass bottle with Parafilm around the top, inside a
larger plastic jar with a corn oil-soaked paper towel taped to the
inside of the lid, and then the outer container is Parafilmed around the
top. No black frig. (Change the oil-soaked towel periodically.)

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Mon Jan 10 17:49:50 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 10 Jan 2000 17:25:02 -0600
Subject: RE: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
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I use Denton spatter coater with Au/Pd target for magnifications
up to 100,000 without any problems. I can observe collagen striations
and other fine details and do not see coating structure.
Parameters for coating: current 15 ma, time 5-10 sec. Some charging can occur
for samples with "multilayer" surface, but it is tough case
for almost any coating anyway. I have also magnetron Cr coater, but
do not use it often because it's much more time consuming.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
} Sent: Wednesday, January 05, 2000 10:15 AM
} To: 'microscopy-at-sparc5.microscopy.com'
} Subject: SEM: Sputter coating
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi,
}
} Due to some recent high-resolution requirements in our lab, I
} find myself
} having to go back to Sputter Coating 101 (after years of just putting
} specimens in the coater and turning it on without a second
} thought!). We
} find ourselves in need of very thin coatings with as little
} structure as
} possible, in order to image samples down in the nanometer range.
}
} We have a chromium coater, but often need to revisit samples
} days or weeks
} after the initial coating. The oxidation problem rears its
} ugly head. We
} intend to purchase a platinum target, but don't yet have one, so we're
} experimenting with our venerable Au/Pd coater.
}
} My questions are:
}
} 1) I seem to remember a string on this listserver suggesting
} that lower
} deposition currents yield finer coating structure. Is this
} right? Does a
} low deposition current for a longer time yield a finer
} coating than a higher
} current for a shorter time? (I'm running some tests to check
} this, but
} would be very interested in others' experiences, too.)
}
} 2) Deposition current can be controlled by the initial current setting
} (i.e., the knob on the machine) and by the argon flow through
} the chamber.
} Is there any difference in the coating when adjusting the
} deposition current
} by either of these two ways?
}
} 3) Charts I have seen indicate that deposition current is directly
} proportional to coating rate. Is the same true for coating
} time? I.e., is a
} one minute coating twice as thick as a 30 sec. coating? It
} would seem so
} intuitively, but you know what they say about the word "assume".
}
} My apologies if these are very basic questions, but, like I
} said, back to SC
} 101!
}
} I'll be happy to summarize the responses for anyone who is interested.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine Bldg.
} University of Missouri
} Columbia, MO 65211
} (573)882-8304
} tindallr-at-missouri.edu
} http://www.biotech.missouri.edu/emc/
}
}



From daemon Mon Jan 10 18:48:38 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Mon, 10 Jan 2000 18:41:35 -0600
Subject: Osmium Vapor- A Safety Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Posted at author's request to remain anonymous....


} To: zaluzec-at-Sparc5.Microscopy.Com
} Date: Mon, 10 Jan 2000 01:20:59 +0530
} Subject: Nestor, would you post this for me?
}
} Osmium Vapor- A Safety Question
}
} The last time I mentioned a safety concern about the rumor of an possible
} "explosive" warning about some Ruthenium compound , I got "Battered" by
} certain vendors(for quite a while too I might add..certainly didn't help
} my previously supportive, pro-vendor position-since I used to be one!) .
} I was warned and pestered frequently and asked to retract any suggestion
} of such a possibility, but I refused. So here we go again....
}
} On approximately January 6, 2000 I noticed a request under the
} title:
}
} : Donna Wagahoff {DWAGAHOFF-at-siumed.edu}
}
} "....Does anyone have experience with osmium stored in
} plastic?..."
}
} and the only responses on the list server were:
}
} " How about putting the glass containers inside plastic containers?
} There are also padded and styrofoam containers which you could use for
} the transportation step."
}
} and
}
} ......" I store this glass bottle inside an aluminum-foiled plastic
} container in my fume hood at room
} temp. The clear plastic of this outer container gets translucent black
} within weeks no matter how carefully I seal the glass bottle".......
}
} ( I'm sure these were very fine suggestions..but..)
}
} This concerns me for several reasons:
}
} 1. I believe there is a considerably more dangerous situation here than
} many of us may realize, or..will discuss. We must check, and we may find
} that Osmium and Ruthenium compounds(and others?) may penetrate all
} plastics and we may only see this at a macroscopic level long after the
} heavy metal compounds or there derivatives have penetrated many layers
} and possible contaminated at some lower and less visible level( but
} undetermined danger level that may be a health risk) a much larger area.
} Many of us may have seen the effect in EM refrigerators as the interior
} blackens over a long period of storage of Osmium.
}
} 2. There doesn't seem to be an acceptable safe level of these compounds
} or known affects, but probably fixation or contamination and loss of
} function at some level occurs(microscopic?).
}
} 3.Also venting these vapors through hoods should be scrubbed or
} absorbed(?), reduced,etc.( "I'm no chemist") and the level of the output
} probably should be carefully and technically monitored.
}
} 4. The safety level must be obvious to those who receive bottles of
} solutions in sealed glass(or Pirex?) and the factories that manufactor
} them.(What glass type materials are safe?)
}
} Does anyone know of the level of danger(or safety) or know of some good
} research sites or periodicals that have dealt with these issues? Any
} personal experience, or are we all afraid of retribution and retaliation?
} The MSDS's seem a little less than thorough to me. I'm sure I'm again not
} alone here in this concern and only wish to continue this difficult and
} frequently dangerous craft(EM) with more care for myself , my associates
} and my community.
}
} Anonymous (chicken....)
} (...and Vendors and manufacturers are our backbone and our support!
} Thank you all!)
}
}
}
}





From daemon Mon Jan 10 18:49:56 2000



From: ELMA Cortinas :      ECORTI-at-childmed.dallas.tx.us
Date: Mon, 10 Jan 2000 18:44:54 -0600
Subject: Negative film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have an idea of a good negative film scanner to use for
TEM negatives? I am trying to eliminate the darkroom printing
process. These are the stipulations:
1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489
film)
2. Must be compatible to a PC.
3. Need to know what software is needed.
4. Must be priced under $3,000.00
5. Must provided the best quality resolution for diagnostic
results.

Thanks in advance!





From daemon Mon Jan 10 18:59:46 2000



From: Mike Mizell :      MMizell-at-CompuServe.COM
Date: Mon, 10 Jan 2000 18:47:22 -0600
Subject: Re: SEM: Sputter Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy;

It is our experience, at South Bay Technology, that Cr films deposited by
ion beam sputtering remain conductive for a short time. The amount of time
depends on the film thickness and sample surface topography. Because Cr
deposition requires a water vapor free environment, usually not possible in
a sputter coater, you may have more success with Ir target. Under 200Kx,
ion beam deposited Ir (better than Pt since Ir films minimize beam damage)
does not display any artifacts (grains cannot be seen) or specimen damage.
We recommend using Cr (or Ti, W or Ta) only when looking at mag } 200Kx.

An Ir target in your sputter coater may work well and an Ir target in your
"Cr coater" may solve the oxidation problem more effectively.

Some advantages of Ion Beam Sputtering:
- Controlled thickness on angstrom level since the average deposition rates
are 10A/min
- Precise thickness measurements reported by quartz thickness monitor as
result of low energy sputterant energy striking crystal
- No damage or artifacts as a result of 30ev sputterant energy
- Any material can be deposited although Cr is suggested for } 200Kx mags,
Ir for {200Kx
- C like metal films are amorphous. C ddoes not display grains or create
damage
- X-ray production from 10A films is lost in the noise
- Image improvement results from increased signal to noise as well as
conductivity

Disclosure: South Bay Technology is the manufacturer of the IBS/E Ion Beam
Sputtering and Etching System and therefore has a vested interested
interest in promoting it use.

Regards, Mike Mizell

*************************************************************************
Michael K. Mizell Tele: 949-492-2600
VP Sales & Marketing Fax: 949-492-1499
South Bay Technology
1120 Via Callejon mizell-at-southbaytech.com
San Clemente, Ca 92673 USA
**************************************************************************
South Bay Technology is an American manufacturer of precision
sample preparation equipment and supplies for metallography
crystallography and electron microscopy.

} } } } } } } Please visit us at http://www.southbaytech.com { { { { { { { {





From daemon Mon Jan 10 21:45:09 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Mon, 10 Jan 2000 16:57:55 -0800 (PST)
Subject: Microtomes available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following items are available to anyone who wants to pay for
shipping or pickup:

American Optical Microtome Knife Sharpener with Grit and Glass Planes

American Optical 820 Rotary Microtome with extra knives

Labline/Hooker (Sledge-type) Plant Microtome

Pauline Yu
pyu-at-pw.usda.gov
510-559-5938
Microscopist Technician
USDA-ARS-WRRC




From daemon Mon Jan 10 21:45:09 2000



From: sumka :      sumka-at-vsnl.com
Date: Mon, 10 Jan 2000 19:06:50 -0600
Subject: what is a nomarski disc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to know what is a nomarski disc. What it is used for? Is there
any difference between this and the routine phase contrast condenser we use
in light microscopy. Regards, M. Angou SUMKA SONS INSTRUMENTATION1137-B,
THADAGAM ROAD,R S PURAM, COIMBATOREINDIA - 641002 FAX: 
91-422-473227TEL:91-422-474378 URL:www.sumka.com





From daemon Tue Jan 11 18:01:06 2000



From: Michael Reiner :      michael.reiner-at-Smail.Uni-Koeln.de
Date: Tue, 11 Jan 2000 17:25:46 +0100
Subject: EM: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members of the list,

first, I would like to wish you all the best for the new year.
Now my question:
Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?

Is it that delicate as LR-White (Meanwhile I don«t use LR-W older than
half a year). My bottle which was not opened many times, could be
roundabout three years old.

Thanks a lot,
Michael

Michael Reiner
Department of Anatomy I
University of Cologne
Germany
michael.reiner-at-smail.uni-koeln.de





From daemon Tue Jan 11 18:01:08 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Tue, 11 Jan 2000 11:52:55 -0500
Subject: Negative film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We scan our 4489 negs with a UMAX PowerLook III regularly on a Mac G3. The
UMAX is compatable with PCs too, cost about 1100 dollars last summer with
the transparancy adaptor and has 1200x2400 dpi actual resolution. We had to
modify the 4x5" transparancy holder (easy) to fit the smaller EM negs. UMAX
tech help could improve but the scanner works well. Stand alone or
PhotoShop plug-in software. Connect via SCSI.

Other units to check out are the Agfa DuoScan T2500 and Microtek ScanMaker 5.

The major electronic trade show meetings are soon so all of the new
'improved' equipment will be ordered and available for summer/fall 2000.
The remaining stock of last year's discontinued models will be discount
priced by Spring.
Good Luck



} Date: Mon, 10 Jan 2000 18:44:54 -0600
} To: Microscopy-at-Sparc5.Microscopy.Com
} From: "ELMA Cortinas" {ECORTI-at-childmed.dallas.tx.us}
} Subject: Negative film scanner
}

}
} Does anyone have an idea of a good negative film scanner to use for
} TEM negatives? I am trying to eliminate the darkroom printing
} process. These are the stipulations:
} 1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489
} film)
} 2. Must be compatible to a PC.
} 3. Need to know what software is needed.
} 4. Must be priced under $3,000.00
} 5. Must provided the best quality resolution for diagnostic
} results.
}
} Thanks in advance!
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax





From daemon Tue Jan 11 18:01:11 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 11 Jan 2000 13:09:22 -0600
Subject: Negative film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Elma,

We are using an Epson Expression 800 and have been very happy with it. We
routinely scan in at 600 dpi, which has been more than enough for
publication quality, in our experience. The unit is capable of higher
resolutions than that. I don't remember what we paid, but it was
considerably less than $3000. It came with a transparency adapter and all
necessary software, including SilverFast, text recognition software,
calibration software, etc. Ours works through Adobe Photoshop's Import
functions, but may be usable in other programs, too.

Best wishes,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine Bldg.
University of Missouri
Columbia, MO 65211
(573)882-8304
tindallr-at-missouri.edu
http://www.biotech.missouri.edu/emc/


-----Original Message-----
} From: ELMA Cortinas [mailto:ECORTI-at-childmed.dallas.tx.us]
Sent: Monday, January 10, 2000 6:45 PM
To: Microscopy-at-Sparc5.Microscopy.Com


Does anyone have an idea of a good negative film scanner to use for
TEM negatives? I am trying to eliminate the darkroom printing
process. These are the stipulations:
1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489
film)
2. Must be compatible to a PC.
3. Need to know what software is needed.
4. Must be priced under $3,000.00
5. Must provided the best quality resolution for diagnostic
results.

Thanks in advance!





From daemon Tue Jan 11 18:01:13 2000



From: Kenneth A. Taylor :      taylor-at-bio.fsu.edu
Date: Tue, 11 Jan 2000 15:39:52 -0500
Subject: Postdoctoral Position Available Immediately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times_New_Roman {/param} {smaller} Postdoctoral
position available immediately to study the 3-D structure of insect
flight muscle. The research project, recently renewed for a 4 year
period, involves several experimental and theoretical approaches to
studying crossbridge structure in different states. Approaches include
electron microscope tomography, alignment and classification of 3-D
crossbridge structures (see recent publication (Winkler & Taylor,
Ultramicroscopy 77:141-152 (1999) for details) and fitting of atomic
coordinates of actin and myosin S1 into the envelope of the 3-D images.
Emphasis is on the study of quick-frozen, contracting muscle,
freeze-substituted for thin section electron microscopy and 3-D image
reconstruction. We use stretch activated muscle as well as an
isometrically contracting state dubbed stretch activation. Specimens
are mechanically monitored right up the point of freezing to facilitate
the interpretation of the structures in terms of muscle force and
stiffness. Parallel X-ray diffraction experiments make for thoroughly
characterized specimens. Please see recent publication (Taylor et al.,
Cell 99:421-431 (1999)) for current status of this project. This
project is a collaboration with Michael and Mary Reedy (Duke Univ. Med.
Center), Yale Goldman and Clara Franzini-Armstrong (U. Penn. Med.
School) and Richard Tregear (MRC, Cambridge, UK). Successful applicants
can work on any of several aspects of the problem of identifying
structural features and relating them to the generation of tension in
muscle. The project has evolved to the point that most of the effort
needs to be put on classification and averaging, model building, model
refinement and interpretation of X-ray data. An individual with
experience in either image reconstruction or protein structure and
function who is interested in gaining further experience in the
interpretation and correlation of diverse experimental data on a
topical biophysical problem would be ideal for this position. The
experimental emphasis of correlating high resolution structures with
lower resolution EM data is expected to become a growth area for
structural biology. Our laboratory is equipped with Silicon Graphics
workstations, one of which is dedicated to this position, a cluster of
3 DEC Alpha compute servers, a Perkin-Elmer PDS 1010M microdensitometer
and a Philips CM300-FEG electron microscope. Salary is commensurate
with relevant experience. Successful candidates will join a strong
Program in Structural Biology with 4 groups using primarily X-ray
diffraction, 3 using NMR, 2 using EPR and one using EM. The Program
enjoys close ties with the National High Field Magnetic Laboratory and
the Supercomputer Computation Research Institute on campus. Additional
information about the Structural Biology Program can be found at
http://www.sb.fsu.edu/. Interested applicants should send their CV and
names, addresses and phone numbers of 3 references to Dr. Kenneth A.
Taylor, Institute of Molecular Biophysics, Florida State University,
Tallahassee, FL 32306, USA. E-mail address is taylor-at-bio.fsu.edu.
Phone number 1-850-644-3357, FAX 1-850-561-1406.


The Institute of Molecular Biophysics and Florida State University are
located in Tallahassee, the capitol of Florida. The city has a
population of ~200,000. The city is surrounded by rolling hills and
pine forests and is 35 miles from pristine beaches on the coast of Gulf
of Mexico. Tallahassee residents enjoy many cultural and sporting
events.



{/smaller} {/fontfamily}
{ { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {}


Kenneth A. Taylor, Ph.D. Office phone: 850-644-3357

Institute of Molecular Biophysics Lab phone: 850-644-4104

Florida State University EM room phone: 850-644-8769

Tallahassee, FL 32306-4380 Fax: 850-561-1406

E-mail: taylor-at-bio.fsu.edu

Home pages: http://www.sb.fsu.edu/~taylor/

http://www.fsu.edu/~biology/faculty/Taylor/kat.html


{ { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {}





{/x-rich}



From daemon Tue Jan 11 18:01:13 2000



From: wft03-at-health.state.ny.us
Date: Tue, 11 Jan 2000 16:25:35 -0500
Subject: Re: Osmium Vapor- A Safety Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Posted at author's request to remain anonymous....

Dear Anon,

} Osmium Vapor- A Safety Question
}
} The last time I mentioned a safety concern about the rumor of an possible
} "explosive" warning about some Ruthenium compound , I got "Battered"...
}
The EM Safety Handbook also warns of the risk of explosion from RuO4.

} On approximately January 6, 2000 I noticed a request under the
} title:
}
} : Donna Wagahoff {DWAGAHOFF-at-siumed.edu}
}
} "....Does anyone have experience with osmium stored in
} plastic?..."
}
} and the only responses on the list server were:
}
} " How about putting the glass containers inside plastic containers?
} There are also padded and styrofoam containers which you could use for
} the transportation step."
}
} and
}
} ......" I store this glass bottle inside an aluminum-foiled plastic
} container in my fume hood at room
} temp. The clear plastic of this outer container gets translucent black
} within weeks no matter how carefully I seal the glass bottle".......
}
} ( I'm sure these were very fine suggestions..but..)
}
} This concerns me for several reasons:
}
} 1. I believe there is a considerably more dangerous situation here than
} many of us may realize, or..will discuss. We must check, and we may find
} that Osmium and Ruthenium compounds(and others?) may penetrate all
} plastics...
}
This is very likely.

} 2. There doesn't seem to be an acceptable safe level of these compounds
} or known affects, but probably fixation or contamination and loss of
} function at some level occurs(microscopic?).
}
The EM Safety Handbook gives a TLV--threshold limit value, defined as
a concentration
"to which *it is believed* (emphasis is mine) *nearly* all workers may be
repeatedly exposed day
after day without adverse effects."--of 2parts in 10^10. Note the very
small value and the cautionary
wording.

} 3.Also venting these vapors through hoods should be scrubbed or
} absorbed(?), reduced,etc.( "I'm no chemist") and the level of the output
} probably should be carefully and technically monitored.
}
Since OsO4 is a powerful oxidant, and since it reacts with unsaturated
lipids, I had heard
the recommendation that corn oil be used to clean up spills; however, the
EM Safety Handbook
recommends ascorbate powder "as it reacts quickly", and, come to think of
it, is more suited for
treating an aqueous solution than is an immiscible oil.

} 4. The safety level must be obvious to those who receive bottles of
} solutions in sealed glass(or Pirex?) and the factories that manufactor
} them.(What glass type materials are safe?)
}
I doubt that any particular glass is less safe (but would not be
surprised to be corrected).
Any glass which doesn't oxidize shouldn't react with OsO4.

} Does anyone know of the level of danger(or safety) or know of some good
} research sites or periodicals that have dealt with these issues? Any
} personal experience, or are we all afraid of retribution and retaliation?

The extensively-quoted EM Safety Handbook is a good source.

} The MSDS's seem a little less than thorough to me.

Although, the existance of an MSDS for di-hydrogen oxide (flamed here
some time ago)
would seem to argue otherwise. There is further info available from
company web sites and phone
lines (listed in some instances on the MSDS), and your safety office should
have other relevant info.

} I'm sure I'm again not
} alone here in this concern and only wish to continue this difficult and
} frequently dangerous craft(EM) with more care for myself , my associates
} and my community.
}
} Anonymous (chicken....)
} (...and Vendors and manufacturers are our backbone and our support!
} Thank you all!)
}
I'd say the first order of safety is to be concerned, next is to get
all the info available.
Yours in chickenhood,
Bill Tivol





From daemon Tue Jan 11 20:28:02 2000



From: c j day :      wa5ekh-at-juno.com
Date: Tue, 11 Jan 2000 19:55:07 -0600
Subject: Vapor Stains-Safety Issue Discussion Please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This technology needs more thorough discussion, I believe. There are
several rather serious safety issues that, I believe, we might find some
better solutions to here, if we open this area for discussion . For
example vapor level and detection, penetration, permeability, scrubbing,
reactive absorption, isolation, etc.. Some plastics stain easily and some
do not, but all are apparently permeable.
On the productive side the commercially astute might find several
new product ideas valuable to this community.
'JD'

previously:

Plastic is oxidized and so part of the osmium is exhausted in the vials.
Even
when frozen osmium diffuses through plastic containers. So you may save a
rare
breakage, but you are certain to have osmium in your refrigerator. It's
just a
bad idea to pack osmium in plastic. You could use a secondary container,
be it
plastic or glass, to increase overall safety.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 06, 2000 3:08 AM, Donna Wagahoff
[SMTP:DWAGAHOFF-at-siumed.edu] wrote:
}
} We have always kept our osmium solutions in glass containers. However,
we
} are in the process of evaluating our safety procedures and discussed
the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the
danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any
comments
} about this particular subject or any of your safety with osmium
procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227





From daemon Tue Jan 11 20:41:33 2000



From: c j day :      wa5ekh-at-juno.com
Date: Tue, 11 Jan 2000 20:24:00 -0600
Subject: ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Recently an E3 Electroscan has become available and I'm becoming aware
of a much different SEM technology than I'm used to seeing over the last
30 years. It obviously operates only at vacuum levels in the torr range,
right? I am still a little confused how resolution can be maintained in
these vacuum levels. And dispersive X-ray spectroscopy, does this
broaden the peaks and what happens to spectral resolution? It also
appears that this particular design cannot pump down below 10-4(?).
There are some complex gas background issues that are different. Are
there ways of using these design parameters to the benefit of the
materials imaging analysis in samples that are not hydrated or partially
volatile?
'JD'/Texas





From daemon Wed Jan 12 07:38:15 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Wed, 12 Jan 2000 11:50:25 +0100 (MET)
Subject: ICVGIP2000 (fwd)

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Our apologies if you receive multiple copies of this announcement.
Please circulate this announcement to your friends and other
researchers.

--------------------------------------------------------------------
Indian Conference on Computer Vision, Graphics and
Image Processing

Dec 20-22, 2000
Bangalore, INDIA

Email: icvgip-at-cair.res.in
URL: http://www.cair.res.in/~icvgip
Phone: +91 80 226 5609 Fax: +91 80 225 5615
--------------------------------------------------------------------

Call for Participation
----------------------

Continuing in the line of ICPIC '95 held at IIT, Kharagpur and
ICVGIP '98 held at IIT, Delhi, ICVGIP 2000 will be organized by the
Centre for Artificial Intelligence & Robotics at Bangalore during
December 20-22, 2000. The conference is intended to bring together
the Vision, Graphics, and Image Processing communities together with
a special emphasis on India. A high quality technical track will be
augmented by presentations from various R&D institutions in the
country and the industry.

Important Dates:
----------------

Submissions due: May 15, 2000
Notifiation of acceptance: Sep 01, 2000
Final papers due: Oct 15, 2000
Conference dates: Dec 20-22, 2000

Topics:
-------

We strive to host a high quality conference in India. An additional
goal his to bring the community of Indian practitioners of these
areas together at a single forum. We encourage papers related to
system development, innovative applications etc., in addition to
research papers. We especially encourage papers by student. The
topics of interest include, but are not limited to, the following:

Computer Vision Image Processing
Computer Graphics Signal Processing
Virtual Reality Multimedia
Document Analysis Pattern Recognition & Matching
Applications Image Processing Architectures
ASIC Design Software & Hardware Tools


Submissions:
------------

Electronic submissions are highly encouraged. Acceptable formats
are: Acrobat PDF, standard PostScript, self-contained LaTeX with
psfig, and Word 7.0. Check the official web page for details on
electronic submission. Manuscripts should not exceed 20
double-spaced pages including figures and tables. The submission
should include a cover page with the title, the authors' names,
abstract and keywords. Those submitting hard-copy manuscripts
should send four copies to the following address:

ICVGIP 2000 Secretariat
Centre for Artificial Intelligence & Robotics (CAIR)
Raj Bhavan Circle, High Grounds
Bangalore, 560 001. INDIA

Further Information:
--------------------

Email address: icvgip-at-cair.res.in
URL: http://www.cair.res.in/~icvgip
Fax: +91 80 225 5615 (Attn: ICVGIP 2000)

--------------------------------------------------------------------
Patrons:
--------
Prof. M. Vidyasagar, CAIR
Prof. R. Narasimha, NIAS

General Chair:
--------------
Dr. P. J. Narayanan, CAIR pjn-at-cair.res.in

Program Co-Chairs:
------------------
Prof. Ramakant Nevatia, USC nevatia-at-usc.edu
Prof. Jayanta Mukherjee, IIT, KGP jay-at-cse.iitkgp.ernet.in

Organizing Chair:
-----------------
Prof. Swamy Manohar, IISc manohar-at-csa.iisc.ernet.in

Plenary Chair:
--------------
Dr. P. Anandan, Microsoft anandan-at-microsoft.com

Publications Chair:
-------------------
Dr. C. V. Jawahar, CAIR jawahar-at-cair.res.in

Treasurer:
----------
Dr. Subrata Rakshit, CAIR subrata-at-cair.res.in

--------------------------------------------------------------------
Organized by Centre for Artificial Intelligence and Robotics (CAIR)
--------------------------------------------------------------------




From daemon Wed Jan 12 07:38:15 2000



From: Greg Ketley :      greg.ketley-at-snet.net
Date: Wed, 12 Jan 2000 06:18:28 -0500
Subject: Denton coater service

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Hi all-
I am looking for someone to provide in house service for a Denton
Sputter Coater in the Rhode Island area. Please feel free to contact me
offline.

Thanks
Greg Ketley
greg.ketley-at-snet.net



From daemon Wed Jan 12 07:38:16 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 12 Jan 2000 08:41:30 -0400
Subject: Re: ESEM (longish)

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----------
} From: c j day {wa5ekh-at-juno.com}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: ESEM
} Date: January 11, 2000 10:24 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Recently an E3 Electroscan has become available and I'm becoming aware
} of a much different SEM technology than I'm used to seeing over the last
} 30 years. It obviously operates only at vacuum levels in the torr range,
} right? I am still a little confused how resolution can be maintained in
} these vacuum levels. And dispersive X-ray spectroscopy, does this
} broaden the peaks and what happens to spectral resolution? It also
} appears that this particular design cannot pump down below 10-4(?).
} There are some complex gas background issues that are different. Are
} there ways of using these design parameters to the benefit of the
} materials imaging analysis in samples that are not hydrated or partially
} volatile?
} 'JD'/Texas
}
} This is exactly the model we've been using here for the past 7 or so
years. In fact, the instrument can be operated in "normal" high vacuum
mode, too (if your samples don't mind it). The resolution is not bad, even
in "wet" mode - say, 2 - 10 torr. There is a certain amount of beam
diffusion, of course, in a wet atmosphere, but we've been doing EDS for the
past 5 or 6 years with pretty good, reproducible, results. (We have a NORAN
ultra-thin window detector and Voyager 3 software.)
Since we have a LaB6 gun in ours, there is also an ion pump, and the gun
vacuum is maintained at a little better than 10 -6 torr.
As you know, the instrument can be used with, instead of water vapour,
inert gases as an atmosphere in the chamber. As it happens, we don't do
that with our instrument, so I couldn't really comment. I suspect that
there probably aren't any major advantages in using an instrument like
this for materials studies, except that it has a very large specimen
chamber that can accomodate several types of stage. And, of course, the
fact that samples generally require no coating before examination. Much of
our usage is earth sciences, and it's nice not to have to coat type fossils
with carbon or metals.
There are two major disadvantages with the E3's. One is that the field of
view is very small - you can't really image anything larger than about 1 mm
in length or diameter, because of the configuration of the ESD
detector/final aperture assembly. This can be a major pain. Another is
that, with the standard stage, samples can not be thicker than about 25 mm
or so, especially if you want to do EDS.
FWIW, our instrument has been dead reliable since it's installation. Other
than biannual column cleanings, the odd hose leak, and an occasional glitch
with some miscellaneous part, the machine is hardly ever down. (Kind of
like my Harley - there may even be a few shared parts :-).
If you do wind up acquiring the E3, the Philips service rep for the
American southeast is (or at least was a couple years ago) Steve Booth.
(You probably know that Philips bought out ElectroScan a few years ago, so
they handle the service contracts now). Steve was here twice to do service
calls on ours, because both times, the regular US Northeast guy was
unavailable. Steve runs a horse ranch somewhere in Texas, I believe, but
knows his E3's pretty well, too.
This might be a whole lot more than you wanted to know, but I'll admit to
being a bit of a fan of our instrument - like my bike, it's ruggedly built,
but is no more complex than it has to be. Just last week, myself and a
local SEM service tech who'd never seen an ESEM before completed a biannual
column cleaning, and it all went very well - takes a day or two to get the
gun vacuum down to operating levels again, though.


No connection with Philips Electron Optics, etc......

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada
B3L 4C8



From daemon Wed Jan 12 08:20:05 2000



From: Giles Sanders :      g.sanders-at-ic.ac.uk
Date: Wed, 12 Jan 2000 07:44:17 -0600
Subject: Fw: CCD Video camera

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Dear List, can you help me once again. Can anyone recommend a sub $1500
video camera for video microscopy.  It will be generally used for
relatively high intensity fluorescence microscopy (imaging GFP bacteria)
and basic microscopy. At present we have a Sony XC-999P (752x582 pixels)
and are looking to upgrade - preferably in resolution and sensitivity -
but resolution is the most important factor. If people wish they can
respond off=list and I will produce a prŽcis of the information I receive.
Thanks.
--------------------------------------------------------------------------------
-----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre
for Analytical SciencesChemistry DepartmentImperial College of Science,
Technology and MedicineLondonSW7 2AY (44) - 0171-594-5749 Never
express yourself more clearly than you think. --  Niels Bohr
(1885-1962) Danish physicist
--------------------------------------------------------------------------------
--------------------





From daemon Wed Jan 12 17:31:53 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Wed, 12 Jan 2000 09:24:09 -0600
Subject: N'tl Geographic/Scharf

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Hello All!
I guess National Geographic Explorer Magazine will be showing some of
David Scharf's work in the next "Explorer" show on TBS (Turner Broadcasting
System-yes it's not the Braves or Clint Eastwood!) on January 16th. They
will show some of his work imaging parasites with the modified
SEM. Should be cool! I love it when they show electron microscopy on TV.
Just thought you might want to know!

Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337




From daemon Wed Jan 12 17:31:54 2000



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Wed, 12 Jan 2000 10:38:50 -0500
Subject: ESEM

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A few of the simpler advantages of an ESEM (we have a model E3):
No need to coat a sample
saves a few minutes (at least)
allows for back-and-forth work with a light microscope
No need to pre-pump to remove volatiles - the differential pumping system
handles this (although your rough pump oil becomes your trap)
Gas evolution (degradation on heating, for example)is not a problem (see
above)
Aging/dynamic studies don't get compromised due to sample coating.
near-atmospheric pressure minimizes sublimation without cryogenics
You can study influence of water (swelling, for example)or other sample/gas
interactions
ESD detector is light-insensitive, so you can watch the sample as you
position it. Also as you poke/prod it with the micromanipulator option.


As for EDX, yes, there is a significant beam spread from the imaging gas. I
typically line up the region-of-interest, then dial the chamber pressure to
zero before collecting spectra. The spread is significantly reduced.

Without trying to touch off arguments, my practical experience is that above
about 20,000X I don't collect images worth writing home about. They are
useful, but not beautiful.

It's an instrument that fills an interesting niche, even for a materials
scientist. (The real forte' is biological/wet stuff. That's where the fun
really begins!)

Bill Heeschen
The Dow Chemical Company

-----Original Message-----
} From: c j day [mailto:wa5ekh-at-juno.com]
Sent: Tuesday, January 11, 2000 9:24 PM
To: Microscopy-at-Sparc5.Microscopy.Com


Recently an E3 Electroscan has become available and I'm becoming aware
of a much different SEM technology than I'm used to seeing over the last
30 years. It obviously operates only at vacuum levels in the torr range,
right? I am still a little confused how resolution can be maintained in
these vacuum levels. And dispersive X-ray spectroscopy, does this
broaden the peaks and what happens to spectral resolution? It also
appears that this particular design cannot pump down below 10-4(?).
There are some complex gas background issues that are different. Are
there ways of using these design parameters to the benefit of the
materials imaging analysis in samples that are not hydrated or partially
volatile?
'JD'/Texas





From daemon Wed Jan 12 17:32:03 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Wed, 12 Jan 2000 11:07:13 -0500
Subject: flat sections

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Does anyone have a method for flattening 0.5 um thick epoxy (Spurr)
sections for LM? We are using water pickup and mild heat drying onto glass
slides and that doesn't seem to be doing the trick.

TIA

Bob
Dr. Robert R. Wise
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(920) 424-3404
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html



From daemon Wed Jan 12 17:31:54 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 12 Jan 2000 10:09:29 -0600
Subject: TEM membrane lipid visualization

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I want to look at what I hypothesize is a densely packed array of
membranes in TEM. In routine osmium fixed, LR White and Epon
embedded specimens, the image is not overwhelming. I plan to try
ruthenium tetroxide ala the skin people looking at lamellar bodies.
I am wondering whether I am missing another obvious approach. Any
ideas gratefully accepted. Tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Jan 12 17:32:00 2000



From: John Balk :      balk-at-kjhsgi.me.jhu.edu
Date: Wed, 12 Jan 2000 13:18:10 -0400
Subject: TEM: preparation of gold foils

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Dear Listers,

I am currently working on preparing TEM foils of single crystalline gold.
The electropolishing was completely fruitless, until I tried Bernie Kestel's
solution "BK-2". This has worked wonders, giving a very smooth and shiny
surface. FYI, I use a Struers Tenupol twin-jet electropolisher, so my
conditions are slightly different than with the South Bay polisher. One
problem remains: the electron-transparent edges of the foil are very prone
to bending, and thus I have regions that are full of bend contours. This
isn't terribly surprising, since the gold is from a grown single crystal and
has been subjected only to about 1% plastic strain. Any ideas on reducing
the amount of bending, so that I can see the dislocations more easily? Are
there any specific profiles for foil perforations that will help keep the
edges rigid (other than a smooth, small hole)? Any ideas, either for
improving specimen prep, or for "fixing" foils I already have, would be
greatly appreciated.

Regards,

John
--
____________________________________
John Balk
200 Latrobe Hall
Johns Hopkins University
3400 N. Charles St.
Baltimore, MD 21218
ph: (410) 516-8284
fax: (410) 516-4316
e-mail: balk-at-kjhsgi.me.jhu.edu




From daemon Wed Jan 12 17:31:57 2000



From: Yuhui_Xu :      Yuhui_Xu-at-hms.harvard.edu
Date: Wed, 12 Jan 2000 12:18:24 -0500
Subject: CCD Cameras for TEM

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Dear Colleagues:

We are in the market for a CCD camera for a JEOL 1200CX TEM. I would
appreciate any information regarding to this type of products available on the
market.
Thank you.

Yuhui




From daemon Wed Jan 12 17:32:01 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Wed, 12 Jan 2000 12:45:29 -0600
Subject: Re: Vapor Stains-Safety Issue Discussion Please

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Those of you who know me can understand that I agree completely with JDs
comment below. The whole issue of safety in handling laboratory chemicals
is one that, I believe, has still not been completely addressed and
accepted by everyone. We all know the short term effect that exposure to
toxic levels of OsO4 can have on a person, eyes is one that comes to
mind. Formaldehyde and glutaraldehyde are others that I and a pathologist,
who I just met yesterday, are now well aware of its possible long term
effects. But what about long term, synergistic effects of some chemicals
that are by themselves relatively innocuous? Combinations of two, three,
four? The only answer is to develop and follow safely procedures for
handling each chemical as if it were very toxic. There is no reason not
to, you already do it for some chemical, and there are many reasons to do so.

For those who don't know me and need a little motivation, think "bilateral,
single sequential lung transplant" .

Damian


At 07:55 PM 1/11/00 -0600, c j day wrote:

} This technology needs more thorough discussion, I believe. There are
} several rather serious safety issues that, I believe, we might find some
} better solutions to here, if we open this area for discussion . For
} example vapor level and detection, penetration, permeability, scrubbing,
} reactive absorption, isolation, etc.. Some plastics stain easily and some
} do not, but all are apparently permeable.
} On the productive side the commercially astute might find several
} new product ideas valuable to this community.
} 'JD'




From daemon Wed Jan 12 17:32:02 2000



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Thu, 13 Jan 2000 07:17:01 +1100
Subject: Re: Vapor Stains-Safety Issue

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What is the longterm effect of exposure to osmium on the brittleness and
permeability of common plastics? We leave exposed polymer block faces in
osmium vapour overnight before sectioning.


Sally



From daemon Wed Jan 12 17:32:02 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Wed, 12 Jan 2000 15:27:11 -0500
Subject: ESEM in DC

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Hello all! We are looking for an ESEM in the greater Washington DC area
for a limited amount of work (maybe a dozen samples over a two-month
period). This could be a contractual situation, although we are rather
hoping for the owner's generosity....
Please reply off-list to: rjpalmer-at-dir.nidcr.nih.gov
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-496-2088
fax 301-402-0396



From daemon Wed Jan 12 17:32:07 2000



From: Harrison, Gail :      Gail.Harrison-at-reichhold.com
Date: Wed, 12 Jan 2000 16:44:43 -0500
Subject: Optical Scope Needs Servicing

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I have a Reichert compound microscope that needs servicing. The number I
have for the company who used to service my microscope is no longer valid.
Is there anyone in the RTP, NC area who could recommend a service vendor?
Please respond to gail.harrison-at-reichhold.com

Many thanks in advance

Gail Harrison
Reichhold
RTP, NC



From daemon Thu Jan 13 07:47:04 2000



From: simon baconnier :      simonb-at-bgumail.bgu.ac.il
Date: Thu, 13 Jan 2000 15:32:39 +0200
Subject: Pineal gland

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Hi,

I'm a post graduate student, and i'm working on the pineal gland, a gland
located in the middle of the brain.

I'd like to know what would be the better way to prpare it for a thin cut
and a hard fixation??

Thank you for answering!!

Simon




From daemon Thu Jan 13 17:49:58 2000



From: Jaci Lett :      jmlett-at-cid.wustl.edu
Date: Thu, 13 Jan 2000 09:20:44 -0600
Subject: STAIN: Toluidine Blue removal

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Does anyone have a solution for this problem? I managed to get a spot ( {3mm
around) of Toluidine Blue on the cuff of my new shirt (the only part
extending beyond my lab coat). Is there any way to get rid of all (or most)
of the stain without it spreading? The shirt is 100% cotton. (I don't care
if it gets on my lab coat, but this is the first time in over fourteen years
that I've gotten it on my clothes.)

If I don't get any response, my inclination is to try a sodium tetraborate
paste applied with a cotton swab.

Jaclynn Lett, Research Assistant jmlett-at-cid.wustl.edu

Fay and Carl Simons Center for the Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO 63110

voice: 314-977-0257 fax: 314-977-0030




From daemon Thu Jan 13 17:50:02 2000



From: Downey, Kevin E :      Kevin.Downey-at-hrl.bsco.com
Date: Thu, 13 Jan 2000 13:42:47 -0500
Subject: TEM Extraction Replicas

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I am having great difficulty extracting large carbides from a steel
specimen using the standard single stage carbon extraction technique.
The film is either not releasing or it is breaking up into unusable
pieces. My standard practice is as follows:

1) Etch polished surface in 2% Nital for 15 to 45 seconds
2) Release sections in 10% Nital
3) Float sections in Dist. water and retrieve

I have used an electrolytic process to aid in releasing the sections
but often this process tends to create somewhat dirty replicas.

I also am going to try a two-stage replication technique, but would prefer
to have sucessful single stage replicas.

Does anyone have any suggestions that would be of assistance in my
single stage replication technique? Thank you for your consideration.

Kevin Downey
Research Analyst
Bethlehem Steel Corp.
e-mail: rkedo-at-bsco.com



From daemon Thu Jan 13 17:50:02 2000



From: pmoore-at-wfubmc.edu (Paula Moore)
Date: Thu, 13 Jan 2000 13:43:54 -0500
Subject: Re: STAIN: Toluidine Blue removal

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} Does anyone have a solution for this problem? I managed to get a spot ( {3mm
} around) of Toluidine Blue on the cuff of my new shirt (the only part
} extending beyond my lab coat). Is there any way to get rid of all (or most)
} of the stain without it spreading? The shirt is 100% cotton. (I don't care
} if it gets on my lab coat, but this is the first time in over fourteen years
} that I've gotten it on my clothes.)

We have some stuff called Erada-Stain. Its made for histological stain removal
from hands, glassware, etc. We've had our tube of it for forever(15 years +)
but it seems like it would be one of those things you should still be able to
find.
Its always worked for me when I had a clothes splash.
Good Luck

Paula Moore
Wake Forest Univ. Baptist Medical Center
EM Lab
pmoore-at-wfubmc.edu




From daemon Thu Jan 13 17:50:02 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 13 Jan 2000 12:56:00 -0600
Subject: TIFF image dpi format ?

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Hello All,

Embeded in TIFF images is data describing the "print" size, X inches by Y
inches at N dpi. Of course, this is directly related to the pixel matrix
size.

My problem is that my digital imaging equipment (Hitachi S-3500 and IXRF
EDS)
saves the image data at 72 (or less) dpi. The pixel array size is correct,
but
at {= 72 dpi, many programs (like PhotoShop) want to print a huge image.

I can overcome this by (in P.S. for example) resetting the image to 300 dpi
and
adjusting the print size so that the pixel array is not altered. At the
very
least, this is cumbersome and time consuming when a large number of images
must
be printed.

I would like to find a way to change the file saving default value for the
dpi
to avoid image resizing for most print applications.

Any suggestions???

Thanks,
Woody White
McDermott Technology



From daemon Thu Jan 13 17:50:03 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 13 Jan 2000 11:27:36 -0800 (PST)
Subject: Mattel QX3 toy microscope

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I've received a lot of inquiries about the education potential of the $100
(or less, in some stores) toy digital microscope introduced by Mattel just
before Christmas; it's a plastic-bodied scope with simple image processing
software which requires a wired connection to a Windows 98 computer (I'm a
Mac user and I don't often feel envious, but...). It's been hard to get
good information, but Jim Harper has just posted an excellent article on
the web. He describes its capabilities well - far better than any of the
other reviews that I've read. And he gives detailed instructions on how to
mount it on ANY light microscope! Don't miss the hotlinks at the end of
his piece.

Educationally, it's no substitute for the one student - one microscope
approach of Project MICRO and "Microscopic Explorations", its manual. But
it has exciting potential for classroom demonstrations and science fair
projects. And listserver readers who are looking for low cost digital
recording of LM may find that it's adequate for a lot of applications.
Please let us know if it works for you.

The site is http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Thu Jan 13 17:50:06 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Thu, 13 Jan 2000 14:36:41 -0600
Subject: SYMPOSIUM CALL FOR PAPERS

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==============================================

ANNOUNCEMENT, AN INVITATION TO OUR SYMPOSIUM:

I am soliciting contributors (or names of potential contributors) for a
symposium for the natiional Microscopy & Microanalysis Annual Meeting to be
held on August 13-17, 2000 in Philadelphia, Pa.

Talks may range in length from 25 to 45 minutes. Deadline for receipt of 2
page absracts is Feb 15, 2000.

A description of the symposium follows.

SYMPOSIUM: MICROORGANISMS: THE GOOD, THE BAD, THE UNUSUAL

This symposium will deal with microorganisms (viruses, bacteria, parasites,
prions) found in the environment as well as in higher life forms (animals
and plants). Newly discovered pathogens or organisms with unique
capabilities (detoxification, invasiveness, resistance to antibiotics) are
of interest in this symposium. Of particular interest are those orgamisms
that represent extremes, as for example: the ability to grow in extreme
environments, having extreme virulence or invasiveness, or being difficult
to visualize using conventional prepartory procedures. Hopefully, the
participants shall describe some of the features of extreme organisms that
give rise to these capabilities. Finally, many of these organisms are often
difficult to visualize using standard preparatory procedures. Papers
describing procedures to prepare the specimens for visualization would be
germane to this symposium.


==============================================







####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################





From daemon Thu Jan 13 17:50:07 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 13 Jan 2000 13:31:24 -0800
Subject: Re: TIFF image dpi format ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hello All,
}
} Embeded in TIFF images is data describing the "print" size, X inches by Y
} inches at N dpi. Of course, this is directly related to the pixel matrix
} size.
}
} My problem is that my digital imaging equipment (Hitachi S-3500 and IXRF
} EDS)
} saves the image data at 72 (or less) dpi. The pixel array size is correct,
} but
} at {= 72 dpi, many programs (like PhotoShop) want to print a huge image.
}
} I can overcome this by (in P.S. for example) resetting the image to 300 dpi
} and
} adjusting the print size so that the pixel array is not altered. At the
} very
} least, this is cumbersome and time consuming when a large number of images
} must
} be printed.
}
} I would like to find a way to change the file saving default value for the
} dpi
} to avoid image resizing for most print applications.
}

I also have a Hitachi S-3500N. Use the Action Palette in PhotoShop to
change the print size and adjust the pixel array with a single key stroke.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Thu Jan 13 17:50:09 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 13 Jan 2000 18:12:08 -0500
Subject: RE: TIFF image dpi format ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suggest getting a hold of ThumbsPlus. I print everything from it. It can
stretch an image to full scale and can print annotation text with the image.
It is a graphics database program that works very well. In addition, it can
convert from almost any format to any other format.

What it can do for you is to convert your image from 72 dpi -big to 300
-small and keep it in the same format, e.g. Tiff. You can do a batch
convert easily.

Find out more at www.cerious.com

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: White, Woody N [mailto:Woody.N.White-at-mcdermott.com]
} Sent: Thursday, January 13, 2000 1:56 PM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: TIFF image dpi format ?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello All,
}
} Embeded in TIFF images is data describing the "print" size,
} X inches by Y
} inches at N dpi. Of course, this is directly related to the
} pixel matrix
} size.
}
} My problem is that my digital imaging equipment (Hitachi
} S-3500 and IXRF
} EDS)
} saves the image data at 72 (or less) dpi. The pixel array
} size is correct,
} but
} at {= 72 dpi, many programs (like PhotoShop) want to print a
} huge image.
}
} I can overcome this by (in P.S. for example) resetting the
} image to 300 dpi
} and
} adjusting the print size so that the pixel array is not
} altered. At the
} very
} least, this is cumbersome and time consuming when a large
} number of images
} must
} be printed.
}
} I would like to find a way to change the file saving default
} value for the
} dpi
} to avoid image resizing for most print applications.
}
} Any suggestions???
}
} Thanks,
} Woody White
} McDermott Technology
}



From daemon Thu Jan 13 20:01:28 2000



From: Dave Phelan :      emudp-at-mail.newcastle.edu.au
Date: Fri, 14 Jan 2000 11:56:52 +1100
Subject: X-Ray mapping problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day

I have a problem doing x-ray mapping on my Philips XL30 (W) with
an attached Oxford ISIS 200 EDS system. I'm running the ISIS
software on the same pc that controls the XL30, a 133 Pentium
with 32Mb memory, running NT4 with service pack 3. I have ISIS
v3.32 and XL v5.5 software. When I try to collect say 6 elemental
maps using the SPEEDMAP software the system locks after 2 or
3 scans and the computer has to be re-booted. I have tried
increasing the memory up to 80Mb but this had no effect. I did not
have this problem when running Windows 3.1, although the system
would give 'out of memory' errors which is why I upgraded to NT.
The problem also occurs when collecting an image using the ISIS
AUTOBEAM software and integrating several frames. Single frame
acquisitions are ok.
If you have a similar system configuration would you please let me
know it you experience this problem? I know of stand alone NT
systems that are ok, so can only assume it is some conflict with
my particular software/hardware combination.

Thanks

Dave







Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au




From daemon Fri Jan 14 07:48:17 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 14 Jan 2000 16:52:03 +1100
Subject: Hitachi FESEMs in NY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello EM Listers,

We are having antifield systems from Lindgren RF Enclosures , fitted on our
two Hitachi FESEMs, an S-4500II and an S-900.

It would be very advisable for the installing engineer to inspect columns
of these models before they set out for Sydney.

Could any operator of these models around New York who would allow
inspection of their microscopes please mail me?

thanks,


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales Sydney, Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Fri Jan 14 07:48:20 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 14 Jan 2000 09:24:55 +0000
Subject: gutaraldehyde safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists

Revisiting an old chestnut - safety and aldehyde fixatives.

In the UK, the Health & Safety Commission have just lowered exposure
limits for glutaraldehyde.

Formalin/formaldehyde has a MEL (maximum exposure limit) of 2 ppm or
2.5 mg/cu m - this is measurable and legally enforcible.
Glutaraldehyde used to have an OES (occupational exposure standard) of
0.2 ppm or 0.83 mg/cu m. over a 15 minute period. OES is a standard to
aim for, but not prosecutable if you weren't achieving it.

Now, glutaraldehyde suddenly has a MEL of 0.05 ppm, both for 15
minute short term reference limit AND THE 8 HOUR TIME WEIGHTED AVERAGE
EXPOSURE!! This is a quarter of what it previously was and 40 times
lower than that for formaldehyde! Does anyone know why or have
evidence or anecdotes of glutaradehyde-related health problems? Is it
so nasty??

I appreciate that it is used in bulk as a bacteriocide in hospitals
and possibly in horticulture/agriculture.

I am trying to contact HSC specialist committees for a response and
will post anything that I receive. I suspect that may be very
little.

Regards - Keith (reincarnated after "early retirement")

PS - Hello, Daniele! And those who know her!
_______________________________
Dr. Keith Ryan
Marine Biological Association of the UK
The Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. ++44 (0)1752 633249
Tel. ++44 (0)1752 633279
The 279 number has an answering machine

Fax ++44 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk




From daemon Fri Jan 14 07:48:23 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 14 Jan 2000 07:34:00 -0600
Subject: TIFF dpi follow-up...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the replies, but...

There may be some confusion about my question. Perhaps I can clarify....

I can resize/fix the image size parameters ok. Either totally manually or
with
a macro from PhotoShop, etc.

My goal is to NOT have to do that. If the original images are SAVED at the
appropriate print dpi this would not be required.... That is my goal. That
is... Is there any way to modify the SAVING software (Hitachi PC-SEM/IXRF
Iridium) so that the images are, for example, 300 dpi rather than 72 or 26
which
is what the software(s) does now.

Woody



From daemon Fri Jan 14 18:31:54 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 14 Jan 2000 09:57:38 -0700 (MST)
Subject: Re: STAIN: Toluidine Blue removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Thu, 13 Jan 2000, Paula Moore wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Does anyone have a solution for this problem? I managed to get a spot ( {3mm
} } around) of Toluidine Blue on the cuff of my new shirt (the only part
} } extending beyond my lab coat). Is there any way to get rid of all (or most)
} } of the stain without it spreading? The shirt is 100% cotton. (I don't care
} } if it gets on my lab coat, but this is the first time in over fourteen years
} } that I've gotten it on my clothes.)
}
} We have some stuff called Erada-Stain. Its made for histological stain removal
} from hands, glassware, etc. We've had our tube of it for forever(15 years +)
} but it seems like it would be one of those things you should still be able to
} find.
} Its always worked for me when I had a clothes splash.
} Good Luck
}
} Paula Moore
} Wake Forest Univ. Baptist Medical Center
} EM Lab
} pmoore-at-wfubmc.edu
}
}
}
Hi,

To destain a slide which has been contrasted with toluidine blue (or any
of the other blues), soak the slide in acid alcohol. To one liter of 70%
ethanol, add 10ml of conc hydrochloric acid. Soak the cuff in that. If
that does not do it, paint your cuff. I have done this frequently. Use
laundry marking stick or magic marker or whatever. If I have a lot of
staining to do, I
simply wear a multicolor blue blouse. No problem.

Hildy Crowley,




From daemon Sun Jan 16 07:07:06 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 14 Jan 2000 09:57:38 -0700 (MST)
Subject: Re: STAIN: Toluidine Blue removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Thu, 13 Jan 2000, Paula Moore wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Does anyone have a solution for this problem? I managed to get a spot ( {3mm
} } around) of Toluidine Blue on the cuff of my new shirt (the only part
} } extending beyond my lab coat). Is there any way to get rid of all (or most)
} } of the stain without it spreading? The shirt is 100% cotton. (I don't care
} } if it gets on my lab coat, but this is the first time in over fourteen years
} } that I've gotten it on my clothes.)
}
} We have some stuff called Erada-Stain. Its made for histological stain removal
} from hands, glassware, etc. We've had our tube of it for forever(15 years +)
} but it seems like it would be one of those things you should still be able to
} find.
} Its always worked for me when I had a clothes splash.
} Good Luck
}
} Paula Moore
} Wake Forest Univ. Baptist Medical Center
} EM Lab
} pmoore-at-wfubmc.edu
}
}
}
Hi,

To destain a slide which has been contrasted with toluidine blue (or any
of the other blues), soak the slide in acid alcohol. To one liter of 70%
ethanol, add 10ml of conc hydrochloric acid. Soak the cuff in that. If
that does not do it, paint your cuff. I have done this frequently. Use
laundry marking stick or magic marker or whatever. If I have a lot of
staining to do, I
simply wear a multicolor blue blouse. No problem.

Hildy Crowley,




From daemon Fri Jan 14 18:31:58 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Fri, 14 Jan 2000 13:43:26 -0600
Subject: Barr bodies in TEM prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings friends,
A researcher wants to know how to stain for, or find by TEM, Barr
bodies that are already prepped and embedded in resin for TEM. Any
advise that you can supply will be most appreciated.
Thanks,
Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-wpo.it.luc.edu



From daemon Fri Jan 14 18:31:59 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 14 Jan 2000 22:01:18 +0000
Subject: Re: TEM Extraction Replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I spent a lot of time many years ago working on 316 stainless. Many
of the specimens I made were single stage carbon extraction replicas.
The technique I found most effective was to use dilute hydrochloric
acid and electrolytic activation. I used this both to etch the
surface prior to carbon coating and also to release the carbon
replica. I also used the same process on ferritic steels. It was very
effective it even released large sheets of M23C6 carbides from grain
boundaries. The only 'precipitate' it would not work on was ferrite
in austenite.

regards,
--
Larry Stoter
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK
email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1825 767967



From daemon Sun Jan 16 07:06:55 2000



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Sat, 15 Jan 2000 11:45:00 +1100
Subject: EM Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position open at the Australian National University , Canberra
www.anu.edu.au/hr/jobs

RESEARCH SCHOOL OF BIOLOGICAL SCIENCES
ELECTRON MICROSCOPY UNIT
ELECTRON MICROSCOPIST
ANU OFFICER GRADE 7 (TECHNICAL)
$43,506 - $47,010 per annum (Plus generous superannuation
provisions)

Reference No: G000011. The ANU Electron Microscopy Unit, a
multidisciplinary research and teaching
support facility with four SEMs, three TEMS and ancillary equipment,
requires a skilled and experienced
person to join its team of 5-6 staff. The successful applicant will have a
history of work in electron
microscopy in a diversified research-oriented environment, preferably with
some administrative experience,
and areas of expertise that support and complement those of existing staff.
They will have up-to-date
expertise in a number of areas of electron microscopy and image analysis.
Among these, experience with
quantitative energy-dispersive X-ray analysis, research projects in plant
or animal cell biology, and
cryopreparation techniques is a necessity.

The ANU EMU website is http://online.anu.edu.au/EMU

Contact for selection documentation: Ms Susan Toscan , ph (02) 6249 4752,
email:
susan.toscan-at-rsbs.anu.edu.au
For further information contact: Dr Sally Stowe, email:
stowe-at-rsbs.anu.edu.au
Closing date: 31st January 2000.




From daemon Sat Jan 15 06:23:38 2000



From: Shaffer-at-physics.niu.edu
Date: Fri, 14 Jan 2000 20:30:14 -0600
Subject: Tenure-Track Faculty Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



NORTHERN ILLINOIS UNIVERSITY

Tenure-Track Faculty Position in Condensed Matter: Electron
Microscopist. The candidate should have a strong background in
transmission electron microscopy and diffraction, and an interest in the
applications of advanced TEM techniques to materials physics. The
candidate would be expected to have a broad knowledge of electron
diffraction theory, of high resolution microscopy and electron
spectroscopy and of materials physics. Possible areas of interest
include (but are not limited to), microscopy of magnetic and/or
superconducting materials, including holography; defects and interfaces
in materials; ferroelectrics; diamond films and film growth; nanoscale
materials; amorphous materials; quantitative microscopy; and 3-D
tomography. Although not essential, an interest in electron optics
would be valuable. The candidate will have the opportunity to establish
a joint program with the Electron Microscopy Center in The Materials
Science Division at Argonne National Laboratory. Send curriculum vitae
and references by March 17, 2000 to: Physics Dept., NIU, DeKalb, IL
60115, Attn: J. C. Shaffer, Chairman. NIU is an AA/EEO Institution.






From daemon Sat Jan 15 06:23:40 2000



From: jean michel Wulveryck :      jm.wulveryck-at-univ-reims.fr
Date: Sat, 15 Jan 2000 15:36:39 +0100
Subject: Information about Beam blanking ....

Contents Retrieved from Microscopy Listserver Archives
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Try Scripps their metrology does contract AFM.

Scripps Institution of Oceanography Analytical Facility

http://sioaf.ucsd.edu/flyer/



----- Original Message -----
} From: {"rfelten-at-Macdermid.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 05, 2000 2:16 PM


Dear colleagues,

Would you please give us some information about a beam blanking device
and a cryostat which can be set inside the microscope chamber. In fact,
we would like to modify our old Phillips microscope (SEM 505) with these
two devices. In particularly, could you give us the quotation for these
two devices,

Thanking you in advance,
Cordially,

email adresse for the answer :
abdelillah.elhdiy-at-univ-reims.fr




From daemon Mon Jan 17 07:16:46 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 16 Jan 2000 09:44:33 -0800 (PST)
Subject: Mattel QX3 toy microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've received a lot of inquiries about the education potential of the $100
(or less, in some stores) toy digital microscope introduced by Mattel just
before Christmas; it's a plastic-bodied scope with simple image processing
software which requires a wired connection to a Windows 98 computer (I'm a
Mac user and I don't often feel envious, but...). It's been hard to get
good information, but Jim Harper has just posted an excellent article on
the web. He describes its capabilities well - far better than any of the
other reviews that I've read. And he gives detailed instructions on how to
mount it on ANY light microscope! Don't miss the hotlinks at the end of his
piece.

Educationally, it's no substitute for the one student - one microscope
approach of Project MICRO and "Microscopic Explorations", its manual. But
it has exciting potential for classroom demonstrations and science fair
projects. And listserver readers who are looking for low cost digital
recording of LM may find that it's adequate for a lot of applications.
Please let us know if it works for you.

The site is http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Mon Jan 17 07:16:54 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 17 Jan 2000 17:32:50 +1100
Subject: position open

Contents Retrieved from Microscopy Listserver Archives
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University of New South Wales,
Sydney, Australia

ELECTRON MICROSCOPE UNIT

Laboratory Assistant

REF. 063NET

FIXED TERM - Total Remuneration: Level 3 (38 hours): A$35,824 - A$41,251
per year. (Salary Level 3: A$30,272 - A$34,858 per year plus up to 17%
employer superannuation plus leave
loading.)

The Electron Microscope Unit is a central infrastructural research
facility, containing nine principal instruments, which support a wide range
of projects. The Unit seeks a self-motivated, enthusiastic person to offer
technical support to assist in the smooth running of the Unit. The
successful applicant will be required to perform a range of routine
laboratory tasks such as film processing, specimen preparation and assist
users of the Unit with operation of microscopes.

Essential criteria: familiarity with the operation of both scanning and
transmission
electron microscopes and with microscope specimen preparation techniques;
previous
experience in research laboratory environment; familiarity with common
windows-based
software packages; good interpersonal skills and a knowledge of EEO/AA
principles.

Desirable criteria: experience with microscopy of biomedical specimens,
experience with
cryomicroscopy techniques; ability to use image processing and analysis
software.
This is a fixed term position to 31 December 2000

Information about the Unit can be found on its website:
http://srv.emunit.unsw.edu.au

Enquiries may be directed to Associate Professor Paul Munroe on telephone
(02) 9385 4435, facsimile (02) 9385 6400 or email: p.munroe-at-unsw.edu.au.

Applications close 28 January 2000.

Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales Sydney, Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Mon Jan 17 07:29:13 2000



From: Alex_Liversage-at-bio-rad.com
Date: Mon, 17 Jan 2000 07:17:37 -0600
Subject: problematic immunogold labelling references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear readers


I have been looking for some relevant references to put in my PhD
thesis which are applicable to the work undertaken.

I was trying to immunogold label (using 5nm gold conjugated to Fab) an
epitope on the giant muscle protein titin within muscle fibre bundles
(all Ab labelling was done prior to sample fixation). However, the
labelling seen was low and inconsistent.

Labelling with FITC conjugated Ab or unconjugated Ab labelled samples
which were then stained was fine though.

Does anyone one know of similar work where immunogold labelling has
failed and possible reasons for this has been explicitly mentioned
within the paper.

Many thanks


Alex Liversage





From daemon Mon Jan 17 19:12:52 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 17 Jan 2000 09:48:05 -0800 (PST)
Subject: Plan Apo Objectives for Leitz Aristoplan

Contents Retrieved from Microscopy Listserver Archives
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I have inherited a Leitz Aristoplan microscope for integration into a
digital imaging system for histopathology. Unfortunately, the mid-range
objectives (10, 25, 40x) are all fluors, although the scope is not equipped
for fluorescence. I would like to acquire planapochromats for each of these
magnifications. The Aristoplan is a fixed tube length (160mm) instrument
that is excellent optically, but the fluotars cause significant vignetting
at all magnifications, even with the correct C mount. If you can provide
any or all of these lenses, please contact me off-list with pricing
information and purchasing details.

Roger Moretz
Dept of Toxicology





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com




From daemon Mon Jan 17 19:12:54 2000



From: ancq3f1nkrz5xza-at-lll.de
Date: Mon, 17 Jan 2000 12:34:37
Subject: Need To Reach Thousands Of Prospects Every Month?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

S P E C I A L R E P O R T -

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_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

Our research has found that many people have tried one or more of
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Free Classifieds? (They just don't work anymore)
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S O W H A T D O E S W O R K ?

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For those not interested in this research, or, if you're
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5 1,050 4,200 50,400
10 2,100 8,400 100,000
15 3,150 12,600 151,200


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_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

F R E E B O N U S E S

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_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

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O R D E R N O W . . . SAME DAY SERVICE (M-F) if your order
is received before 2pm Pacific. 24hour fax service, just fax
to: 1-435-404-1340

To order, via credit card simply cut/paste and print out the
EZ ORDER FORM below and fax to our office today.

***** MILLIONS CD - Volume 7 *****

***** NOW ONLY $235! *****

This "Special Price" is in effect for the next seven days,
after that we go back to our regular price of $299.00 ...
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We accept Visa, Mastercard, Amex and Checks by Fax.
Fax your order to: 1-435-404-1340

----------------------Cut & Paste----------------------
---------------------EZ Order Form---------------------

_____Yes! I want everything! I am ordering within 72 hours.
Include my FREE "Business On A CD" bonus along with your 10
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addresses) for the special price of only $235.00 + s&h.

_____I missed the 72 hour special, but I am ordering Vol. 7,
10 Million, super clean e-mail addresses, within 7 days for the
"special" price of only $235.00 + s&h.

_____Oop's I missed the 72 hour and 7 day "specials". I am
ordering Vol. 7 at the regular price of $299.00 + s&h.

***PLEASE SELECT YOUR SHIPPING OPTION***

____I would like to receive my package FedEx OVERNIGHT. I am
including $15 for shipping. (Hawaii & Alaska $20 - Canada $25,
all other International add an *additional* $25 [$40 total] for
shipping)

____I would like to receive my package FedEx 2 DAY delivery.
I'm including $10 for shipping. (Sorry no Alaska, Hawaii, Canada
or International 2nd day delivery - Continental U.S. shipping
addresses only).

***Please Print Carefully***

NOTE: Orders cannot be shipped without complete information
including your signature. No exceptions!


NAME____________________________________________________

COMPANY NAME____________________________________________

ADDRESS_________________________________________________
(FedEx can only ship to street addresses - no P.O. boxes)

CITY, STATE, ZIP________________________________________

PHONE NUMBER____________________________________________
(required for shipping & tracking)


EMAIL ADDRESS___________________________________________
(Print Carefully - required in case we have a question and to
send you a confirmation that your order has been shipped)

TYPE OF CREDIT CARD:

______VISA _____MASTERCARD _____AMEX

CREDIT CARD# __________________________________________

EXPIRATION DATE________________________________________

NAME ON CARD___________________________________________

TOTAL AMOUNT (Including Shipping): $___________________

DATE:x__________________

(Required) SIGNATURE:x_________________________________
I understand that I am purchasing the Millions Vol. 7 e-mail
address CD, the addresses are not rented, but are mine to use for
my own mailing, over-and-over. Free bonuses are included, but
cannot be considered part of the financial transaction. It is
the users responsibility to comply with any laws applicable to
their local area. As with all software, once opened the CD may
not be returned, however, if found defective it will be replaced
with like product at no charge.

You may fax your order to us at: 1-435-404-1340

CHECK BY FAX SERVICES!

Please Note: Sorry, we can only accept checks drawn on U.S.
banks.

If you would like to fax a check, tape your check below and
fax it to our office along with the EZ Order Form to:
1-435-404-1340

******************************************************

***24 HOUR FAX SERVICES*** PLEASE PASTE YOUR

CHECK HERE AND FAX IT TO US AT 1-435-404-1340

*******************************************************

If You fax a check, there is no need for you to mail the
original. We will prepare a one-time draft, with the exact
information on your original check. All checks will be
held for bank clearance. (7-10 days) Make payable to:
"CD-Marketing"





























********************************************************
Do not reply to this message -
********************************************************
To register your e-mail address for future removal, visit:
http://www.OptList.com/register.html.
********************************************************



From daemon Tue Jan 18 07:43:45 2000



From: Stephen Griffiths :      s.griffiths-at-ucl.ac.uk
Date: Tue, 18 Jan 2000 12:59:53 -0000
Subject: LM: Large slides for large sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone

One of our Ph.D students has to mount some large sections of flattened
visual cortex.
The sections are about 10 to 12 cm square.

I cannot find any supplier of microscope slides, that big, in our
catalogues.

Does anyone know of a supplier/manufacturer of large glass slides?
They must exist, surely?
UK supplier preferred, but anyone, anywhere if necessary.

TIA

Stephen
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Stephen Griffiths
Visual Science Department
Institute of Ophthalmology
Bath Street, London. EC1V 9EL
e-mail:- s.griffiths-at-ucl.ac.uk (work)
or stephen.griffiths-at-dial.pipex.com (home)
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}




From daemon Tue Jan 18 18:53:24 2000



From: Evans Stephen SJ :      Stephen.Evans-at-aguk.zeneca.com
Date: Tue, 18 Jan 2000 14:58:48 -0000
Subject: LM: Efficient clearing of Arabidopsis seed coats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello!

Not having a lot of microscopy experience, I was wondering if anyone could
recommend some solutions for effectively clearing Arabidopsis seed coats. I
would like to use as non-toxic a solution as possible (no Xylene!) and also
one which works quickly. Can anyone give me some handy hints?

Thanks for you help!

Stephen Evans
Wheat Improvement Centre
Norwich Research Park
Colney
Norwich NR4 7UH
stephen.evans-at-aguk.zeneca.com




From daemon Thu Jan 20 07:35:25 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Tue, 18 Jan 2000 10:40:29 -0600
Subject: Getting back to you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Roy:
Sorry I haven't gotten back with you sooner, but I was off for the
holidays. Do you still need equipment? Give me a call so we can
discuss what you need.
Regrards,
Mike Coviello
817 272-5496



From daemon Tue Jan 18 18:53:27 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Tue, 18 Jan 2000 12:34:22 -0500
Subject: re: pump lifetimes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I received this question from a co-worker. Please respond directly to me.

thanks in advance,
Marisa Ahmad

--------------------

Small question. We have a Leybold mechanical pump hooked to a Reactive Ion
Etcher. It is running 24 hrs/ day, and pumps actively on the chamber about
35 times per day. How often should a pump under these conditions be rebuilt?
The reason I am asking is the pump seems to be blowing seals and requires
rebuilding about once a year, the manufacturer says this is normal...is it?
If not, what should we do to improve the time between rebuilds?



From daemon Wed Jan 19 08:04:47 2000



From: Dr G. R. Coulton [bs_mp] :      g.coulton-at-ic.ac.uk
Date: Wed, 19 Jan 2000 10:16:23 +0000
Subject: 11th International Congress of Histochemistry and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael Davidson at Florida State University has an excellent demonstration
of the QX3 microscope at:
http://microscopy.fsu.edu

Don O'Leary
----- Original Message -----
} From: "Caroline Schooley" {schooley-at-mcn.org}
To: {Microscopy-at-Sparc5.Microscopy.Com}
Sent: Sunday, January 16, 2000 12:44 PM


11th International Congress of Histochemistry and Cytochemistry (ICHC 2000)

ON-LINE REGISTRATION NOW AVAILABLE


Dear Collegues,

I have mentioned before the upcoming meeting ICHC 2000 3-8 Sept. 2000 York, UK

There will a range of sessions that will I am sure be of great interest to
this group. Please take a look at our web-site at
http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you their

Best wishes

Gary Coulton
Organiser ICHC 2000



Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

tel 0044 (0)171 594 3190
fax 0044 (0)171 594 3022

e-mail g.coulton-at-ic.ac.uk

-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Understanding Biocomplexity: The Post-Genome Challenge"

September 3-8, 2000, York, United Kingdom

ICHC 2000 will comprise 27 symposia addressing the latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

On-line registration now open!!!!!!!!!!

For further details of the meeting and how to pre-register please visit our
web-site at http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you there.



From daemon Wed Jan 19 19:14:41 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Wed, 19 Jan 2000 10:02:34 -0500
Subject: Need Sony printer drivers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




john grazul wrote:

} Fellow Microscopists,
}
} I "found" a Sony Mavigraph UP-D7000 Digital Color Printer with 2 cases
} of consumables. What I could not find is any of the software to run the
} little beast; and besides it was suppose to go on a Unix system, so
} even if I found the software it would be useless {I think}.
}
} I went to the Sony Web site and found no downloads, any other
} suggestions or even some discs etc... would be a great help. BTW, the
} Sony will be on a PC.
}
} Thanks,
}
} John Grazul
} Lucent




From daemon Wed Jan 19 19:14:46 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 19 Jan 2000 12:18:50 -0500
Subject: Martian summer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I thought I'd beat Tina to the weather report. Here in
Albany, NY (where cryo-microscopy means working with the
windows open) we are having Martian summer--yesterday's
high was -15 C.
Yours,
Bill Tivol




From daemon Wed Jan 19 19:14:47 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 19 Jan 2000 13:11:22 -0500 (EST)
Subject: Robert Derby search

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry to bug everyone, but I'm trying to reach Robert Derby. If you are
out there, please e-mail me! Or if anyone has a contact address for him,
could you send it to me?

Thanks!

Tamara Howard
CSHL





From daemon Wed Jan 19 19:14:47 2000



From: Beth Dickey :      ecdickey-at-engr.uky.edu
Date: Wed, 19 Jan 2000 13:41:02 -0500
Subject: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times_New_Roman {/param} {bigger}

{/bigger} {/fontfamily} {bigger} {bold} {fontfamily} {param} Times {/param} {bigger} RESEARCH
ASSOCIATE

Electron Microscopy Facility

University of Kentucky


{/bigger} {/fontfamily} {/bold} {fontfamily} {param} Times {/param} {bigger} The
University of Kentucky invites applications for a Research Associate in
the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286

{/bigger} {/fontfamily} {/bigger}

***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/



{/x-rich}



From daemon Wed Jan 19 19:14:48 2000



From: UIC Network Services Kit User :      netid-at-machine.cc.uic.edu
Date: Wed, 19 Jan 2000 12:43:40 -0600
Subject: frozen sections, need help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I am trying to get frozen sections of rat skin about 10 um thick.
The tissue was perfusion fixed w/ 4% para in PBS then cryoprotected in
sucrose/ PBS and mounted with OTC compound. More often then not my
sections are sticking to the knife edge and are hard to remove even with
a brush. I'm a bit knew to cryo-sectioning so any tips would be greatly
appreciated.
Thanks, Andy




From daemon Wed Jan 19 19:14:48 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 19 Jan 2000 09:04:38 -1000 (HST)
Subject: Re: Martian summer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Listers,
} I thought I'd beat Tina to the weather report. Here in
} Albany, NY (where cryo-microscopy means working with the
} windows open) we are having Martian summer--yesterday's
} high was -15 C.
} Yours,
} Bill Tivol


OK, OK, it's in the 70sF, raining with enough sun for spectacular
rainbows, and there's a break in the winter North Shore surf
season. But I'm stuck in a windowless lab just like the rest of you guys!

http://wavetrak.surfline.com/pipecam.asp

My condolences on your winter blues.

Aloha,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Wed Jan 19 19:14:50 2000



From: John Bonevich :      john.bonevich-at-nist.gov
Date: Wed, 19 Jan 2000 15:38:24 -0500
Subject: Re: Need Sony printer drivers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, I have version 1.1 of a plugin for Adobe Photoshop
(Macintosh). I can send you a copy to try. You'll be printing
"pink" images in no time.

John Bonevich


} john grazul wrote:
}
} } Fellow Microscopists,
} }
} } I "found" a Sony Mavigraph UP-D7000 Digital Color Printer with 2 cases
} } of consumables. What I could not find is any of the software to run the
} } little beast; and besides it was suppose to go on a Unix system, so
} } even if I found the software it would be useless {I think}.
} }
} } I went to the Sony Web site and found no downloads, any other
} } suggestions or even some discs etc... would be a great help. BTW, the
} } Sony will be on a PC.
} }
} } Thanks,
} }
} } John Grazul
} } Lucent


--------------------------
John Bonevich, Ph.D.
NIST, Metallurgy, Stop 8554
100 Bureau Drive
Gaithersburg, MD 20899 USA
TEL: (301) 975-5428
FAX: (301) 975-4553



From daemon Wed Jan 19 19:14:52 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 19 Jan 2000 13:42:19 -0800 (PST)
Subject: Mattel QX3 "toy" microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please don't consider this a "commercial" posting; my goal is to encourage
lots of experimentation. The Mattel microscope
(http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html )lists for $100,
but Toys-R-Us is now selling it for $69.95, in both its retail stores and
website. And if you go to www.etoys.com, you can download a Mattel $30
rebate certificate, good till 4/29. So it looks like you can get one for
~$40. Have fun, folks!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Wed Jan 19 19:14:54 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 19 Jan 2000 19:43:21 -0500
Subject: Anti-vibration platform for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Micro-g anti-vibration platform that our JEOL 1200EX TEM was
sitting on until recently. This allowed us to do decent microscopy on a
vibration prone second floor with very good results. We no longer have a
need for this unit. It could be used with a TEM or SEM.

What we would like to do is trade it for a new Mac. We can not buy Macs.
If you need a platform, let's talk trade. I think that this would be a good
deal.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Wed Jan 19 23:19:53 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Wed, 19 Jan 2000 17:29:23 -0800
Subject: Freeze Fracture retirement sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
We have a Balzars 301 Freeze Fracture with quartz thin film monitor, double
replica accessory, and electron guns for both platinum and carbon up for
grabs. New seals on the mechanical pump. Original equipment bought circa
1977. Last used 1998, and was perfectly fine. Free, as is. Recipient will
pay for moving.
Kristen

Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Wed Jan 19 23:30:53 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 19 Jan 2000 23:20:22 -0600
Subject: confocal vs deconvolution microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
We're investigating both confocal microscopy and epi-fluorescence
combined
with de-convolution software for a multi-user facility. I'm requesting
input from directors and managers of other shared technology laboratories
regarding systems chosen and whether the system has met their expectations.
Please include websites which include FAQs and clarification of terms.
Thanks in advance for your input.
Rosemary Walsh
EM Facility for the Life Sciences
Life Science Consortium & Biotechnology Institute
Penn State University
University Park, PA. 16802
(814) 865-0212





From daemon Thu Jan 20 07:25:21 2000



From: pogany-at-power.szfki.kfki.hu (Pogany Lajos)
Date: Thu, 20 Jan 2000 10:06:12 +0100
Subject: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear ALL,
there is a lot of good news about the QX3 microscope (toy-)attachment, and I
would like to try it under Windows95 or Windows NT4. Is there any driver for
this systems?
any thanks
Lajos Pogany




From daemon Thu Jan 20 07:25:22 2000



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Thu, 20 Jan 2000 10:33:03 +0100
Subject: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear fellow microscopists,

I have the task to analyse (by EELS technique) precipitates in steel.
I would greatly appreciate some hints coming from people who are
already familiar with the difficulties connected with:
1. carbon analysis. It is obvius that a carbon film support for the
precipitates extracted from the steel is to be avoided. Has anyone
experince on the use of other kind of supporting film
(silicium monoxide ?, beryllium ?). I have the same problem with
using copper grids, because the precipitates are supposed to
contain copper too. What kind of grids is most appropriate?

2. would it be a better solution to perform precipitate analysis on
thinned steel specimens ? Can it occurr an annoying interference
originating in the material surrounding the precipitate ?

3. last but not least, I would greatly appreciate some bibliographical
hints on that very specific topics.

Thank you in advance.

Corneliu Sarbu
MTM Dept. of KULeuven, Belgium



From daemon Thu Jan 20 18:25:28 2000



From: Donald Delaney :      delaneyd-at-mcw.edu
Date: Thu, 20 Jan 2000 08:25:58 -0600
Subject: 2 questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List

I have two questions to ask the List

1) Does anyone have a used TEm ultrathin microtome for sale and if so, how
much?

2) Does anyone know refernces or protocols for both immunogold labelling
and microwave embedding?





From daemon Thu Jan 20 18:25:28 2000



From: Dan Kremser :      dkremser-at-levee.wustl.edu
Date: Thu, 20 Jan 2000 08:50:19 -0600
Subject: Biological Standards for Microprobe Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Recently, an interesting medical school project has appeared at my lab
door. I've been asked to quantify Ca and P in scar tissue found in
sections of hamster hearts. Obtaining appropriate standards is critical.
Are there commercially available biological standards or procedures to
create such standards out there? Any suggestions would be appreciated and
thanks for your time.

Dan



=====================================================
Dan Kremser
Washington University
Department of Earth and Planetary Sciences/Campus Box 1169
(Wilson Hall, Room 108-----for packages)
One Brookings Dr.
St. Louis, MOÊ 63130-4899

VOICE: (314) 935-5605Ê FAX: (314) 935-7361
E-MAIL: dkremser-at-levee.wustl.edu



From daemon Thu Jan 20 18:25:36 2000



From: Anita Garg :      Anita.Garg-at-lerc.nasa.gov
Date: Thu, 20 Jan 2000 10:51:15 -0500
Subject: Re: glue for cross-section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues
What is the best glue for making cross-section samples to study ~ 30
micron reaction layer formed by a coating on a Ni-base superalloy?
The M-bond 610 which used to work great for Si and ceramic samples
does not seem to work here. Though the glue I have is pretty old.
TIA
Anita



From daemon Thu Jan 20 18:25:38 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Thu, 20 Jan 2000 11:32:10 -0500
Subject: Need Recommendations for Digital Image Database/organization Soft

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone have any recommendations for organizing digital images and associated
data in electronic form in a GLP environment? I'm going crazy trying to find
something that will be easy to use, will link the image with associated data
information, and will have restriction or audit capabilities to track any
changes to the data that may be made.

Any input is greatly appreciated.

Gregg Sobocinski
Parke-Davis Pharmaceuticals
Ann Arbor, Michigan
USA
Gregg.Sobocinski-at-wl.com





From daemon Thu Jan 20 18:25:38 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Thu, 20 Jan 2000 12:00:18 -0500
Subject: Re: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} What about the Mac? :-)
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




John Mansfield PhD CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"



From daemon Thu Jan 20 18:25:45 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Thu, 20 Jan 2000 12:54:27 -0500
Subject: MAT: making silicon oxide layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I need to make some ~100nm thick oxide layers on silicon to align an ion
gun in an auger system. I assume that most people use thermal oxidation to
grow the films.

Does anyone have a recipe for making these films? (time, temperature,
atmosphere) It would be really great if the recipe allowed me to get an
exact thickness too!

Thanks,
Henk

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
An optimist believes that we live in the best of all possible worlds.
A pessimist fears that this is true.




From daemon Thu Jan 20 18:25:40 2000



From: anderron-at-us.ibm.com
Date: Thu, 20 Jan 2000 13:17:14 -0500
Subject: Re: glue for cross-section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


According to the data sheet packed with M-Bond 610, the room temp pot life,
after mixing, is six weeks.

We note that it fails quickly, i.e. it works fine one day and doesn't the
next. As we can't abide samples coming unglued, we toss mixed M-Bond 610
after 30 days and mix fresh.

Also, M-Bond 610 works best bonding smooth surfaces. If we have rough
surfaces we use GATAN G-1 (Epo-Tec 353ND).

Ron



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



Anita Garg {Anita.Garg-at-lerc.nasa.gov} on 01/20/2000 10:51:15 AM

To: Microscopy-at-sparc5.microscopy.com
cc:


Dear Colleagues
What is the best glue for making cross-section samples to study ~ 30
micron reaction layer formed by a coating on a Ni-base superalloy?
The M-bond 610 which used to work great for Si and ceramic samples
does not seem to work here. Though the glue I have is pretty old.
TIA
Anita







From daemon Thu Jan 20 18:25:40 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 20 Jan 2000 08:27:24 -1000 (HST)
Subject: Re: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} } What about the Mac? :-)

Remember - this thing was developed by Intel!


Aloha,
Tina

It's raining cats and dogs, and it's cold and miserable. Feel better,
now?

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Thu Jan 20 18:25:42 2000



From: MICHAEL MOHN :      MMOHN-at-mail.monroe.cc.mi.us
Date: Thu, 20 Jan 2000 14:24:07 -0500
Subject: LM-Bausch & Lomb Research II Metallograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have technical manuals or documentation for a B&L Research II Metallograph which I could copy. MCCC just received one as a donation.
Thanks!

Michael Mohn
Assistant Professor of Materials Technology
Monroe County Community College
Phone: 734-384-4122 Fax: 734-242-9711
http://www.monroe.cc.mi.us/mmohn




From daemon Thu Jan 20 18:25:46 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 20 Jan 2000 17:51:29 -0400
Subject: Re: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Thu Jan 20 18:25:47 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 20 Jan 2000 17:08:23 -0500
Subject: Re: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I attempted to do this many years ago. I made replicas using aluminium. This
was written up in the proceedings of a conference on microanalysis
("Measurement
of carbon in V(C,N) precipitates extracted from HSLA steels in aluminum
replicas," by A.J. Garratt-Reed, in "Quantitative Microanalysis with High
Spatial Resolution," The Metals Society, London, Book No. 277, 1981, p. 165.)
Then someone else tried to replicate my work (for the moment I can't remember
his name, but he was at Strathclyde University in Glasgow) and could not get
consistent results. He concluded that the aluminum was somehow catalysing the
oxidation of the carbon in the carbides. I expect he published the results,
but I can't now remember where. I have some memory that he used silicon
monoxide to make replicas, but when I tried that, the replicas would break up
because of beam-induced charging. Of course, it would have defeated the point
to have put a carbon coat on the films!

When copper has been an issue, I have often used nickel, which are very
satisfactory. You can also get grids of many other materials, including Ti,
Al, Au, Mo, etc., etc. - check your EM suppies catalogues.

Tony G-R.




At 10:33 AM 01/20/2000 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Thu Jan 20 18:25:47 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 20 Jan 2000 17:44:19 -0500
Subject: RE: making silicon oxide layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Henk,
Ta is usually used for measuring sputter rates and you can see where the
beam was put. You electrochemically grow a specific thickness and measure
the sputter rate. If you want to pursue this, I can give you a referral to
a couple of surface scientists who have done this. They are in your neck of
the woods.

Another thing that you might want to try is something that I wrote up a
number of years ago in JVST as a shop note for aligning an ion gun system
where I could not see the beam. Take Double sticky tape and put it on your
sample holder. It works in a UHV system well enough, trust me. Then push
the sample holder into yellow WO3 powder to cover the sticky tape. Where
the beam hits the sample, the WO3 will turn blue. A couple of sample
transfers and you have it.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--


} -----Original Message-----
} From: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu]
} Sent: Thursday, January 20, 2000 12:54 PM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: MAT: making silicon oxide layers
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi all,
}
} I need to make some ~100nm thick oxide layers on silicon to
} align an ion
} gun in an auger system. I assume that most people use
} thermal oxidation to
} grow the films.
}
} Does anyone have a recipe for making these films? (time,
} temperature,
} atmosphere) It would be really great if the recipe allowed
} me to get an
} exact thickness too!
}
} Thanks,
} Henk
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://web.ceof.ohio-state.edu
} An optimist believes that we live in the best of all possible worlds.
} A pessimist fears that this is true.
}
}



From daemon Thu Jan 20 19:45:45 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 20 Jan 2000 18:40:25 -0500
Subject: RE: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I read Tony's answer to your question and I can't add anything to that.
However, I just have a minor point. If you are using EELS, why are you
worried about the grids? The grids would not show up in the EELS spectrum.
Of course it would interfere if you are using EDS.

You can get your grids in almost any material you want nowadays. Ni, Mo,
Be, Al, Cu, C, etc. Just pick one that doesn't interfere with your EDS
spectrum.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be]
} Sent: Thursday, January 20, 2000 4:33 AM
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: steel precipitates analysis by EELS
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear fellow microscopists,
}
} I have the task to analyse (by EELS technique) precipitates in steel.
} I would greatly appreciate some hints coming from people who are
} already familiar with the difficulties connected with:
} 1. carbon analysis. It is obvius that a carbon film support for the
} precipitates extracted from the steel is to be avoided. Has anyone
} experince on the use of other kind of supporting film
} (silicium monoxide ?, beryllium ?). I have the same problem with
} using copper grids, because the precipitates are supposed to
} contain copper too. What kind of grids is most appropriate?
}
} 2. would it be a better solution to perform precipitate analysis on
} thinned steel specimens ? Can it occurr an annoying interference
} originating in the material surrounding the precipitate ?
}
} 3. last but not least, I would greatly appreciate some bibliographical
} hints on that very specific topics.
}
} Thank you in advance.
}
} Corneliu Sarbu
} MTM Dept. of KULeuven, Belgium
}



From daemon Thu Jan 20 18:41:07 2000



From: Gaener Rodger :      grodger-at-molbiol.ox.ac.uk
Date: Thu, 20 Jan 2000 18:32:55 -0600
Subject: information about current TEM and Confocal technologies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear users,

I am celating information about current TEM and Confocal technologies with
the intention of setting up both a confocal suite and an EM suite at a new
facility. However, I am new to this field and would like some advice on
the pros. and cons. of different systems based on other user experiences,
to give me an idea of what I should be looking out for. Specifically, the
confocal suite would allow the study of both live (EGFP) and fixed
samples. Thus, the type of confocal needed should have good resolution
for both live and fixed samples as well as good phase contrast.
Importantly, this will probably be a multi-user facility, which needs
reliable lasers that do not need to be realigned often. It would also be
important to have good quality microscope, filters and objectives as well
as extras such as a heated stage, video and CCD cameras, appropriate
computer workstations and software. Any advice that you could offer on
such equipment would be very much appreciated.

Thanking you in advance,


Dr Gaener Rodger.

-------------------------------------------------------------------

Dr Gaener Rodger
Sir William Dunn School of Pathology
University of Oxford
South Parks Road
Oxford
UK.





From daemon Thu Jan 20 19:15:46 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Thu, 20 Jan 2000 19:08:26 -0600
Subject: Microscopy Society of America - Council Election Results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The results of the recent election of officers to the Society are now official
The current list of elected officers is below.



The new officers elected this year are:

President-Elect
RON ANDERSON

Secretary
JANET H. WOODWARD (2000-2002)

Director, Physical Sciences
THOMAS F. KELLY (2000-2002)

Director, Biological Sciences
SARA E. MILLER (2000-2002)

-------------------------------------------
The complete list of officers is given below
and on the MSA WWW site
http://www.msa.microscopy.com/MSADocs/MSAOfficers.html
--------------------------------------------



MSA COUNCIL 2000

President
KEN DOWNING
326 Donner Lab
Lawrence Berkeley Lab
Berkeley, CA 94720
(510) 486-5941; Fax (510) 486-6488
Email: khdowning-at-lbl.gov

President-Elect
RON ANDERSON
IBM Analytical Services
IBM Zip-41E
Hopewell Junction, NY 12533
(914) 892-2225; Fax (914) 892-2555
E-mail: ron-anderson-at-vnet.ibm.com

Past-President
DAVID JOY
Rm.232 Science and Engineering Research Facility
Univ. of Tennessee
Knoxville, TN 37996-0810
(865) 974-3642, Fax (865) 974-9449
and
Rm. S189, High Temperature Materials Laboratory
Oak Ridge National Laboratory
Oak Ridge, TN 37831-6064
(865) 574-6799
E-mail: djoy-at- utk.edu

Secretary
JANET H. WOODWARD (2000-2002)
Buckman Laboratories, Inc.
1256 N. McLean Blvd.
Memphis, TN 38108-1241
(901) 272-6408; Fax (901) 272-6451
E-mail: jhwoodward-at-buckman.com

Treasurer
KATHI ALEXANDER (1999-2001)
Los Alamos National Lab
MST-8 G755 P. O. Box 1663
Los Alamos, NM 87545
(505) 665-4750, Fax (505) 667-8021
Email: kbalexander-at-lanl.gov

Directors, Physical Sciences

J. MURRAY GIBSON (1998-2000)
Argonne National Laboratory
Materials Science Division
Argonne, Ill 60439 {p}
Email:gibson-at-anl.gov

THOMAS F. KELLY (2000-2002)
2021 Chamberlain Ave.
Madison, WI 53705-4076
(608) 263-1073; Fax (608) 262-8353
E-mail: tfkelly-at-engr.wisc.edu

MIKE KERSKER (1999-2001)
JEOL USA
11 Dearborn Rd
Peabody MA 01960
(508) 535-5900 Fax (508) 536-2205
E-mail: kersker-at-jeol.com

Directors, Biological Sciences
SARA E. MILLER (2000-2002)
Duke Medical Center, Pathology
Box 3712
Durham, NC 27710
(919) 684-3452; Fax (919) 684-8735
E-mail: saram-at-acpub.duke.edu

AVRIL SOMLYO (1998-2000)
Dept. of Molecular Physiol. & Bio. Phys.
Box 10011, Health Sciences Center
Univ. of Virginia
Charlottesville, VA 22906-0011
(804) 982-0825; Fax (804) 982-1616
E-mail: avs5u-at-virginia.edu

JOHN BOZZOLA (1999-2001)
EM Ctr - Mailcode 4402
Southern Illinois Univ
Carbondale IL 62901
(618) 453-3730, Fax (618) 453-2665
E-mail: bozzola-at-siu.edu

Director, Local Affiliated Societies
EV OSTEN (2000-2002)
3M Company
3M Center, Bldg. 201-BE-16
St. Paul, MN 55144-1000
(651) 736-0104; Fax (651) 733-0648
E-mail: efosten-at-mmm.com


==============================================
Nestor
Your Friendly Neighborhood SysOp






From daemon Fri Jan 21 07:34:51 2000



From: Debbie Nieuwenhuis :      watersci-at-sauk.com
Date: Thu, 20 Jan 2000 20:01:02 -0600
Subject: reticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was wondering if anyone could tell me how to properly install a reticle in
an Olympus microscope eyepiece. The eyepiece housing does not seem to come
apart, although there are two tiny round holes on either site of the lens.
Is there a special tool needed?

Thanks!

Deb




From daemon Fri Jan 21 10:20:51 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 21 Jan 2000 10:55:08 -0500
Subject: RE: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have to admit that until I read Scott's posting, I had missed the point
about the elemental interference from the grid not being a problem with
EELS analysis.

However, in the case of copper, there is another effect to worry about when
making replicas, if the process involves any sort of flotation on, or
picking the sample up from, water. Because of its electronegativity,
copper can dissolve in the water and then ion-exchange with other elements
from the sample, replacing them. Then you really do have copper in the
sample. The problem isn't particularly severe with extraction replicas
from steels (but then, I have never used extraction replicas from steels to
try to analyze for small amounts of copper in the precipitates), but has
been terrible, for example, when looking at iron sulphides, which actually
had a visible shell of copper sulphide (but I was also fishing those
samples out of brine, not distilled water!). The problem doesn't seem to
occur with nickel grids, which I use in preference to copper for this type
of application, just to be on the safe side.

BTW, I'm glad you got my posting, Scott - I haven't seen it come back to
myself yet. I guess the poor mail redirector gets indigestion with the
volume it has to deal with!

Tony.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Fri Jan 21 18:19:15 2000



From: Robert Derby :      rjderby-at-excite.com
Date: Fri, 21 Jan 2000 08:22:55 -0800 (PST)
Subject: Me

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


************************************************
Robert J. Derby
New Mexico Institute of Technology
Socorro, N.M.
Phone - 505-835-5866
E-mail - rjderby-at-excite.com
************************************************
I have heard through the grapevine that Tamara Howard from Cold Spring
Harbor is looking for me.
I was told it was posted here but I never got it.
Tamara call me at 505-835-5866(Iam 2 hours behind you)
Robert





_______________________________________________________
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Visit http://freeworld.excite.com




From daemon Fri Jan 21 18:19:15 2000



From: Neal Leddy :      nleddy-at-tcd.ie
Date: Fri, 21 Jan 2000 16:27:58 -0000
Subject: Polymerising Epoxy Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Group,

Just a quick question, I was wondering if anyone had ever tried using a
microwave oven to harden their resin??? And if so what sort of results were
achieved.
Another question I have is how to remove / dissolve hardened resin from an
aluminium surface???

I would appreciate any suggestions or advice.
Thank you

Neal Leddy




From daemon Fri Jan 21 18:19:16 2000



From: Ford M. Royer :      froyer-at-bitstream.net
Date: Fri, 21 Jan 2000 10:32:09 -0600
Subject: TEM Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone interested in the following TEMs FOR SALE?

Phillips 201C
Complete system, fully operational, taken out of service in December.

JEOL model JEM-100B. - Parts Unit Only

--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
800-565-1895 phone
612-929-1895 fax
web site: http://www.aibltd.com





From daemon Fri Jan 21 18:19:16 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 21 Jan 2000 17:19:40 +0000
Subject: glutaraldehyde safety - summary (M)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all

Many thanks to all who responded on and off-line to my request for
information. I hope I acknowledged each one individually.

My quest was to find the real reason for lowering the exposure limit.
I won't summarise the replies but: (1) pulmonologists at one hospital
are thinking that long term, low level exposure to formaldehyde and
glutaraldehyde may cause idiopathic pulmonary fibrosis, (2) I was told
of a serious skin burn caused by a splash.

I have now heard from the UK Health & Safety Commission's Advisory
Committee on the Toxicity of Substances (ACTS). I have copy from: HSE
Review 1997, published 1999, section C58 (consisting of 4 pages).
Quotes from this are below.

The official reason for lowering the exposure limit is that it has
not been possible to set a no-observed adverse effect level for
glutaraldehyde with regard to the induction of asthma.

Carcinogenicity is not supported by the available good-quality
evidence to date.
________________________________________

Glutaraldehyde must be labelled under the Chemicals (Hazard
Information and Packaging for supply) Regulations 1994 (CHIP) as
Toxic, Corrosive, Sensitising and Dangerous for the Environment.

Several thousand tonnes are imported into the UK each year. It is
primarily used as a biocide and disinfectant in the health care,
off-shore, paper-making and agricultural sectors.

.. it is estimated that a considerable number of (health care)
workers are intermittently exposed, given the widespread use in that
sector. Similarly, ....... for several hundred workers in the
manufacture of glutaraldehyde solutions.

..... available exposure data relates mainly to use in the health
care sector....... suggests that under normal operational conditions
short term exposures are generally less than 0.2 ppm. This can be
exceeded during the cleaning of endoscopes ...... or the wiping of
surfaces.

HEALTH EFFECTS - ANIMAL STUDIES.
Glutaraldehyde is acutely toxic to rats by inhalation, oral and
dermal routes. The principal effects are due to its irritant
properties.

Glutaraldehyde is clearly a skin sensitiser in rabbits, guinea pigs,
rats and mice.

Glutaraldehyde is clearly mutagenic in vitro, in bacterial and
mammalian cells. ............. No firm conclusions can be drawn from
the available evidence on chromosomal aberrations, but glutaraldehyde
clearly causes sister chromatid exchange (SCE) and unscheduled DNA
synthesis (UDS) in mammalian cells.

......... However, the clearly negative results of recent,
good-quality bone marrow cytogenetics and peripheral blood
micronucleus tests, together with those of the liver UDS assay,
provide reassurance that the genotoxic effects shown in vitro are
unlikely to be expressed in vivo.

No reports of carcinogenicity studies of glutaraldehyde by the
inhalation or dermal routes of administration are currently available.
A recent oral study provided no convincing evidence ... in rats .....
drinking water for up to two years.

No significant effects on reproduction were reported in a modern two
generation study ........... There were no indications of significant
gonadal effects in 13 weeks inhalation studies carried out in rats or
mice, or in a lethal assay in mice.

HUMAN DATA
Glutaraldehyde is irritant to to human skin at concentrations of
2-10%, but not 0.5%. Higher concentrations have not been
investigated.

There is substantial evidence that glutaraldehyde is a skin
sensitiser in humans. Concentrations as low as 0.13% have induced
allergic contact dermatitis. The majority of cases have been reported
in health or funeral workers.

A fair body of evidence ............. indicates that glutaraldehyde
has the potential to cause occupational asthma.

.......... several workplace studies with exposure data in which no
cases of asthma have been found among contemporary workers. .....
However, superimposed on these data are other reports of sensory
irritation and / or asthma in endoscopy nurses where the reported
levels of exposure overlap with those in the above studies.
.................. From the data available, it has not been possible
to determine a NOAEL (no-observed adverse effect level) for the
induction of asthma.

No information is available in genotoxicity in humans.

The only available mortality study is of limited value, but does not
provide any evidence that glutaraldehyde caused cancer or increased
mortality in glutaraldehyde production workers.

On the basis of the very limited information available, it does not
appear that glutaraldehyde causes reproductive toxicity in humans.

REFERENCE: Glutaraldehyde: Criteria document for an occupational
exposure limit EH/65/32. HSE Books ISBN 0 7176 1443 3.
_________________________________________________

Dr. Keith Ryan
Marine Biological Association of the UK
The Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. ++44 (0)1752 633249
Tel. ++44 (0)1752 633279
The 279 number has an answering machine

Fax ++44 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk

PS - Daniele, you're still here! Keep smiling!! That was a nice
evening in Strasbourg. Do you know which Gewurztraminer we all had as
an aperatif? Now the world will wonder?!




From daemon Fri Jan 21 18:19:21 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 21 Jan 2000 14:18:24 -1000 (HST)
Subject: Images to PowerPoint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


************************************************
Robert J. Derby
New Mexico Institute of Technology
Socorro, N.M.
Phone - 505-835-5866
E-mail - rjderby-at-excite.com
************************************************
Does anyone or any company know of a ccd that will capture single photon at
the 852-872 wavelength?
This is needed for a special project.
Any help would be of great help
Thanks,

Robert





_______________________________________________________
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Visit http://freeworld.excite.com



1256 N. McLean Blvd.
Memphis, TN 38108-1241
(901) 272-6408; Fax (901) 272-6451
E-mail: jhwoodward-at-buckman.com

Treasurer
KATHI ALEXANDER (1999-2001)
Los Alamos National Lab
MST-8 G755 P. O. Box 1663
Los Alamos, NM 87545
(505) 665-4750, Fax (505) 667-8021
Email: kbalexander-at-lanl.gov

Directors, Physical Sciences

J. MURRAY GIBSON (1998-2000)
Argonne National Laboratory
Materials Science Division
Argonne, Ill 60439 {p}
Email:gibson-at-anl.gov

THOMAS F. KELLY (2000-2002)
2021 Chamberlain Ave.
Madison, WI 53705-4076
(608) 263-1073; Fax (608) 262-8353
E-mail: tfkelly-at-engr.wisc.edu

MIKE KERSKER (1999-2001)
JEOL USA
11 Dearborn Rd
Peabody MA 01960
(508) 535-5900 Fax (508) 536-2205
E-mail: kersker-at-jeol.com

Directors, Biological Sciences
SARA E. MILLER (2000-2002)
Duke Medical Center, Pathology
Box 3712
Durham, NC 27710
(919) 684-3452; Fax (919) 684-8735
E-mail: saram-at-acpub.duke.edu

AVRIL SOMLYO (1998-2000)
Dept. of Molecular Physiol. & Bio. Phys.
Box 10011, Health Sciences Center
Univ. of Virginia
Charlottesville, VA 22906-0011
(804) 982-0825; Fax (804) 982-1616
E-mail: avs5u-at-virginia.edu

JOHN BOZZOLA (1999-2001)
EM Ctr - Mailcode 4402
Southern Illinois Univ
Carbondale IL 62901
(618) 453-3730, Fax (618) 453-2665
E-mail: bozzola-at-siu.edu

Director, Local Affiliated Societies
EV OSTEN (2000-2002)
3M Company
3M Center, Bldg. 201-BE-16
St. Paul, MN 55144-1000
(651) 736-0104; Fax (651) 733-0648
E-mail: efosten-at-mmm.com


==============================================
Nestor
Your Friendly Neighborhood SysOp





--CAB19553.948442177/styx.services.ou.edu--


} From MAILER-DAEMON Fri Jan 21 02:09 CST 2000
Received: from localhost (localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with internal id CAA00349; Fri, 21 Jan 2000 02:09:34 -0600


Hello, all-

I'm hoping to hear from those of you who have worked out the optimum size
and resolution to make images in e.g., Photoshop that are destined for
PowerPoint to be made into transparencies. An image that is 4 x 5 inches
and 400-600 dpi seems to be overkill for a 35mm slide.

Your opinions?

Mahalo,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Fri Jan 21 18:49:25 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 21 Jan 2000 18:35:08 -0600
Subject: Inconel 718 Polish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nick:

I found the following recipe from Bernie Kestel. He states that this is
used for surface polishing using the beaker method. Although he did not
use it for "jet polishing", it may be a good starting point.

Inconel 718 (annealed)
10% HClO4
90% Ethanol
Temperature = -60C
Current: 275mA
Volts: as required

There is some more information about stir rate, sample orientation etc.
that is only relevant for the beaker method. If you'd like to try this
approach, I would be happy to send you the entire reference.

I hope this helps.

Best regards-

David
Writing at 10:14:39 AM on 01/21/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Schryvers Dominique
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm looking for a good electropolishing solution + conditions for
as-received and annealed Inconel 718 for use with a Tenupol 3 system to
produce well thinned matrix + precipitates for TEM work. Any suggestions?

Nick Schryvers






From daemon Sat Jan 22 11:28:25 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 22 Jan 2000 09:55:54 -0600
Subject: Re: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

A minor observation ...........

While the issue of overlaps is generally not a problem with EELS (as
opposed to EDS), if can arise with steel precipates. The vanadium L
and Oxygen K edges are really quite close, so if you have vanadium
precipatates, a silicon oxide support film will cause problems for
quantitation.

regards,
--
Larry Stoter
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK
email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1825 767967





From daemon Sat Jan 22 11:28:25 2000



From: COURYHOUSE-at-aol.com
Date: Sat, 22 Jan 2000 12:19:05 EST
Subject: Re: Martian summer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yikes! Great! set the microscope near the window and get those snow flake
pictures! It has been close to 80 degrees a few days this week here in
Arizona!
I have a great batch of infusion brewing on the back patio.

Got the happage win TV card to add to the computer....Have the c-mount ccd
camera I found for $100.... now... I should be able to share pictures soon!

Ed Sharpe
archivist for SMECC

{ { Subj: Martian summer
Date: 1/22/00 7:50:37 AM Pacific Standard Time
From: tivol-at-wadsworth.org (William Tivol)
Sender: tivol-at-wadsworth.org
To: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Dear Listers,
I thought I'd beat Tina to the weather report. Here in
Albany, NY (where cryo-microscopy means working with the
windows open) we are having Martian summer--yesterday's
high was -15 C.
Yours,
Bill Tivol
} }



From daemon Sun Jan 23 16:27:39 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 24 Jan 2000 01:16:18 -0600
Subject: Administrivia: Server Replaced this weekend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The main Microscopy Server was replaced this weekend (read
that as many blurry eyed hours in front of a monitor, and lots
of cursing at new formats of configuration files for DNS and SENDMAIL).

The old beast was growing increasing unreliable with hardware
crashes nearly daily. I believe that most of the services have been
restored however until all databases are reconfigured and tested there
will be some glitches. Please be patient.

I think I have been able to capture the few messages that were sent
over the weekend. If those of you that posted items over the weekend
don't see things in the next day please repost them.

Cheers...

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Jan 24 07:52:03 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 24 Jan 2000 01:26:50 -0600
Subject: Re: Images to PowerPoint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tina,
Our medical photography dept. requests that files submitted for
slides are at least 1 Mb and no more than 3 Mb in size. Less than this
doesn't have enough pixels to adequately fill the image (similar to a thin
negative), and more is unneeded. I create the slide figure, set to 300 dpi
and adjust dimensions to put the file size into that range.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

On Fri, 21 Jan 2000, Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} I'm hoping to hear from those of you who have worked out the optimum size
} and resolution to make images in e.g., Photoshop that are destined for
} PowerPoint to be made into transparencies. An image that is 4 x 5 inches
} and 400-600 dpi seems to be overkill for a 35mm slide.
}
} Your opinions?
}
} Mahalo,
} Tina
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
}




From daemon Mon Jan 24 07:52:03 2000



From: Pnina Ari-Gur :      arigurp-at-wmich.edu
Date: Mon, 24 Jan 2000 01:26:54 -0600
Subject: Faculty Position Materials Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position: Full/Associate/Assistant Professor of Materials Science and
Engineering. This is a full time, tenure track teaching and research
position beginning Fall 2000.

Rank and Salary: Rank will depend upon background and experience; salary
competitive.

Responsibilities: Teach undergraduate and graduate courses in materials
science and engineering, advise undergraduate and graduate students,
seek and conduct funded research, supervise theses and projects, and
serve on university, college and department committees.

Academic
Qualifications: Earned Ph.D. in materials science and/or engineering or
a closely related field is required. Demonstrated experience in research
and grant seeking is highly desired. Prior teaching experience a plus.

Experience
Qualifications: Demonstrated experience in research and grant seeking is
highly desired. Prior teaching experience a plus.

Department: The Department of Construction Engineering, Materials
Engineering and Industrial Design at Western Michigan University
currently offers two undergraduate BSE -- Materials Engineering and
Construction Engineering and Management, and BS in Industrial Design.
The Department also offers two Master of Science programs - Materials
Science and Engineering and Construction Management.

University: Western Michigan University, with a student body of
approximately 28,000 is located in southwest Michigan. Kalamazoo is
halfway between Chicago and Detroit and 45 miles south of Grand Rapids.
The population of the greater Kalamazoo area is approximately 200,000.
Its industry is highly diversified and it is the center of many cultural
and sporting events.

Applications: Review of applications will begin on January 10, 2000, and
will continue until the position is filled. Please send the following
credentials: Letter of Application addressing qualifications, Vitae,
Transcripts from all institutions, and the names/addresses,
telephone/fax numbers of three references

Contact: Please send credentials to:

Dr. Roman Rabiej, Chair
Western Michigan University
Department of Construction, Materials, & Industrial Design
2007 Kohrman Hall
Kalamazoo, MI 49008

AN EQUAL OPPORTUNITY/AFFIRMATIVE ACTION EMPLOYER
Western Michigan University is an Equal Opportunity Employer. In
addition, it has embarked upon a vigorous affirmative action program and
encourages the applications of women and members of minority groups.
==============================
Pnina Ari-Gur, D.Sc., Professor of
Materials Science and Engineering
Western Michigan University
Kalamazoo, MI 49008
(616) 387-3372 FAX: (616) 387-6517
email: pnina.ari-gur-at-wmich.edu
http://www.wmich.edu/cmd/arigur.htm
==============================




From daemon Mon Jan 24 07:52:02 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 24 Jan 2000 01:26:57 -0600
Subject: PCD for JEOL 840 wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy New Year

Does anyone have a Probe Current Detector accessory for a JEOL 840
surplus to requirements and available for purchase?
I think its designation is PCD40, it's the pneumatically-operated
thing which mounts on the column opposite to the objective aperture
holder, and shoots a Faraday cup across into the beam.

thanks

Ritchie Sims


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand




From daemon Sun Jan 23 16:27:44 2000



From: de Lillo Enrico :      delillo-at-agr.uniba.it
Date: Mon, 24 Jan 2000 14:41:14 +0100
Subject: Re: EURAAC meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Fashing,
it is a pleasure receiving news from you. I have to apologize for the
trouble you have reported in your letter and I hope to help you.

First of all, the website is going to be updated because of the second
circular is ready to be sent.

The second point is that you have enough time to submit your paper or
poster. The deadline for submission has been established on 30 April 2000.
I hope you answered to the first circular (my database has not been
recently updated - Migliorini is working on this and he has the complete
and updated archive), so in this case you will receive the second circular
directly on your computer. Otherwise I suggest you to fill the application
on the web site.

Let me know if I could help you more. I will be glad to do that.

My best wishes and see you in Siena


Enrico


} Dear Dr. De Lillo,
}
} I am interested in attending the EURACC symposium in Siena this July,
} and would greatly appreciate receiving information concerning that
} meeting. I keep checking the meeting homepage on the web site
} (http://www.unisi.it/ricerca/dip/bio_evol/sitoeuraac/siena2000.html),
} but very little information is given. I also contacted the e-mail
} address (euraac2000-at-unisi.it) a few months back and have not yet
} received a reply. I wrote to Dr. Leo P. S. van der Geest, the EURAAC
} Secretary, yesterday and he gave me your e-mail address and thought
} perhaps you could help.
}
} I would like to present a paper at the meeting (probably a poster)
} and need to know when titles and abstracts are due and to whom they
} should be sent. Also the format that should be used for submitting
} the paper.
}
} Many thanks for your help; it is greatly appreciated. I look forward
} to hearing from you.
}
} Sincerely,
}
} Norm
}
} Norm Fashing
} Professor of Biology
} Department of Biology
} College of William and Mary
} P.O. Box 8795
} Williamsburg, VA 23187-8795
} 757 221-2221 (Office)
} 757 221-6483 (FAX)
} njfash-at-facstaff.wm.edu
} http://www.wm.edu/biology/Fashing.html


dr Enrico de Lillo
Istituto di Entomologia agraria - Universitˆ Bari - Italy
via Amendola, 165/A - 70126 Bari - Italy
tel. +39 080 5443105
fax +39 080 5442876
email: delillo-at-agr.uniba.it
http://193.204.185.103/de_lillo.htm




From daemon Mon Jan 24 08:04:35 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Mon, 24 Jan 2000 06:46:26 -0700
Subject: Reichert Ultracut E with cryo FC 4D

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The power supply in our cryo microtome is having problems which might be
related to the transformer. I was told by the service engineer that the
transformer is no longer supported by Reichert. Does any one know where I
can get a replacement ?

Thanks

Jordi Marti



From daemon Mon Jan 24 15:07:21 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 24 Jan 2000 11:04:49 -0400
Subject: Re: Administrivia: Server Replaced this weekend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nestor,
Thanks for taking such good care of us.
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jan 24 15:07:23 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Mon, 24 Jan 2000 10:05:22 -0500
Subject: Ignore This Message (Testing)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a test to see if my messages are being posted to the listserver.




From daemon Mon Jan 24 15:07:24 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Mon, 24 Jan 2000 10:35:13 -0500
Subject: Need Recommendations for Digital Image Database/organization Soft

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone have any recommendations for organizing digital images and associated
data in electronic form in a GLP environment? I'm going crazy trying to find
something that will be easy to use, will link the image with associated data
information, and will have restriction or audit capabilities to track any
changes to the data that may be made.

Any input is greatly appreciated.

Gregg Sobocinski
Parke-Davis Pharmaceuticals
Ann Arbor, Michigan
USA
Gregg.Sobocinski-at-wl.com



From daemon Mon Jan 24 15:07:24 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Mon, 24 Jan 2000 10:41:36 -0500
Subject: RE: Inconel 718 polish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Contact Robin Griffin at UAB. I bought that unit there and we worked on 718
and developed the polishing conditions for it. I believe that we used the
butyl cellusolve(SP?)/perchloric acid solution at low temp to do it. She
should have the recipe or know who to contact. Her Email address is
rgriffin-at-eng.uab.edu or you might get a hold of Ray Thompson whose student
did the work. As I recall, the as-cast material was very difficult to do
and had very narrow conditions. The annealed samples were a little easier.
To save time, we had the samples initially cut out of the bulk samples using
an EDM machine.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Schryvers Dominique [mailto:schryver-at-ruca.ua.ac.be]
} Sent: Friday, January 21, 2000 2:53 AM
} To: Microscopy MAIL
} Subject: Inconel 718 polish
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I'm looking for a good electropolishing solution + conditions for
} as-received and annealed Inconel 718 for use with a Tenupol 3
} system to
} produce well thinned matrix + precipitates for TEM work. Any
} suggestions?
}
} Nick Schryvers
}
}
} *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
} *=* *=*
} *=* Dr. D. Schryvers *=*
} *=* Electron Microscopy for Materials Research (EMAT) *=*
} *=* University of Antwerp, RUCA *=*
} *=* Groenenborgerlaan 171 *=*
} *=* B-2020 ANTWERP *=*
} *=* Belgium *=*
} *=* tel: 32-3-2180247 *=*
} *=* fax: 32-3-2180257 *=*
} *=* e-mail: schryver-at-ruca.ua.ac.be *=*
} *=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
} *=* *=*
} *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
}
}



From daemon Mon Jan 24 15:07:25 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 24 Jan 2000 09:45:39 -0600
Subject: Re: Images to PowerPoint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The short answer to you question would be an image of about 1024 pixels
across should be adequate for a slide used for a presentation. The slide
will appear to most people as a 8x10"-print held at arm's length, or as a
computer screen at 4 to 6 feet. If you cannot make out the pixels in those
images, then you probably have enough pixels in your image for PowerPoint.

I think I start seeing the pixels when the resolution drops to 800 or 640
pixels across. I might see a benefit in raising the pixels to 1280 across,
but I am not able to see improvement beyond that point. This would give you
an image of about 1 million pixels.

Now if you are shooting your slide with a 35-mm camera, it might be good to
use 24-bit color on the image if your output device (e.g., printer) can
well render it. However, since the slide is only for a presentation, you
can probably get by with much less color depth if file size per slide is an
issue. I venture to say that 8-bit color at 1024 pixels across is plenty
adequate for most presentations.

Remember, the above considerations are only for images for slide
presentations. If the slides are meant to archive the images for other
purposes, then you probably want every bit of resolution and color depth
that your technology allows and justifies.

Warren

At 02:18 PM 1/21/2000 -1000, you wrote:
} Hello, all-
}
} I'm hoping to hear from those of you who have worked out the optimum size
} and resolution to make images in e.g., Photoshop that are destined for
} PowerPoint to be made into transparencies. An image that is 4 x 5 inches
} and 400-600 dpi seems to be overkill for a 35mm slide.
}
} Your opinions?
}
} Mahalo,
} Tina



From daemon Mon Jan 24 15:07:34 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Mon, 24 Jan 2000 14:24:57 -0400
Subject: just a test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just checking to see if I'm still "wired to the world".....


F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada B2Y 4A2



From daemon Mon Jan 24 15:07:34 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 24 Jan 2000 13:33:28 -0500
Subject: Bio Cryo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Periodically questions appear on this list concerning the use of cryostats
for cutting histological sections. I usually reply to the sender off line
and offer a copy of a handout that I got from a workshop at a Histochem
Meeting some years ago. Even though it is old, cryostat sectioning has not
changed a lot. I think there is some valuable info there, especially for
beginners. I thought it might be helpful to make this available on the net
for whomsoever might want to take a look.
It can be found at :
http://www.biotech.ufl.edu/sems/

Look for the snowflake

It was written by Bruce Quinn, then of MIT. I hope he has no objections
to my posting it.

Greg Erdos
Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Mon Jan 24 15:07:34 2000



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Mon, 24 Jan 2000 13:51:22 -0500
Subject: EM technician position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TECHNICAL POSITION AVAILABLE

A full time position is available immediately for a highly motivated
individual to work in the Center for C. elegans Anatomy at the Albert
Einstein College of Medicine, located in the Bronx, New York. The
candidate should have a Bachelor's degree in Biology or some related
science, and some previous laboratory experience. We are particularly
looking for an individual with training in transmission electron microscopy
and thin section microtomy. Experience with immunocytochemistry and/or
computerized image analysis is helpful but not required.

The College offers a generous compensation package including 4 weeks
vacation and tuition reimbursement. Qualified candidates should submit a
resume and a list of references to:

Dr. David Hall
Department of Neuroscience
Albert Eistein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

email: hall-at-aecom.yu.edu
FAX: 718 430-8821
phone: 718 430-2195



From daemon Mon Jan 24 15:07:36 2000



From: Cieslinski, Robert (RC) :      RCCIESLINSKI-at-dow.com
Date: Mon, 24 Jan 2000 13:33:07 -0600
Subject: uProbe - Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Available:

Electron Microprobe Analyst

Company: The Dow Chemical Company

Location: Midland, Michigan

Qualifications (education, certification, language, etc.) and Experience
required:
A candidate with a BS or MS or PHD degree in physical science is preferred.
Prior experience in electron probe microanalysis is essential.


Job Overview:
The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D Analytical
Science Laboratory has one professional level full time opening for an
electron microprobe analyst. The candidate should have theoretical and
practical experience in electron beam techniques, including quantitative
x-ray microanalysis, digital imaging, digital x-ray imaging, electron
beam/solid interactions, scanning electron microscopy and material science.
Good computer skills are very desirable. Good written and oral
communication skills and the ability to work both independently and in a
team environment are extremely important.


Key responsibilities will include:
1. Extensive problem solving on a wide variety of Dow materials and
processes
2. Operation and routine maintenance of a CAMECA SX-50 electron microprobe.
3. Some sample preparation including microtomy and metallography
4. Operation of light microscopes.
5. Operation of scanning and transmission microscopes as needed.
6. Interpretation of images.
7. Documentation of work.
8. Compliance with safety and quality systems


Interested:
Please e-mail or send your resume and cover letter, with reference to this
ad to:
Email: R&D-at-Dow.com or The Dow Chemical Company, Workforce Planning, P. O.
Box 150, Plaquemine, Louisiana 70765-0150. E-mail respondents must list Job
006145 and their last name as the first and second items on the Subject
line. Only those selected for an interview will be contacted. Only U.S.
citizens or aliens who are authorized to work in the United States will be
considered for employment.

We are an equal opportunity employer and offer a competitive compensation
and benefits package including 401k, stock purchase, tuition reimbursement
and performance incentives. The Dow Chemical Company is the fifth largest
chemical company in the world with annual sales of US$20billion. Dow
manufactures and supplies chemicals, plastics and agricultural products for
customers in 164 countries and employs approx. 43,000 people worldwide. For
more news and information about Dow, please visit our web site at
www.dow.com.






Robert C. Cieslinski
The Dow Chemical Company
Microscopy & Microanalysis
(517) 636-6875
email: rccieslinski-at-dow.com



From daemon Mon Jan 24 15:07:39 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Mon, 24 Jan 2000 13:08:38 -0700
Subject: Need Recommendations for Digital Image Database/organization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We successfully import our digital images into a PDF file using Adobe
Acrobat.

Harry Ekstrom

-----Original Message-----
} From: Sobocinski, Gregg [mailto:Gregg.Sobocinski-at-WL.com]
Sent: Monday, January 24, 2000 8:35 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Anyone have any recommendations for organizing digital images and associated
data in electronic form in a GLP environment? I'm going crazy trying to find
something that will be easy to use, will link the image with associated data
information, and will have restriction or audit capabilities to track any
changes to the data that may be made.

Any input is greatly appreciated.

Gregg Sobocinski
Parke-Davis Pharmaceuticals
Ann Arbor, Michigan
USA
Gregg.Sobocinski-at-wl.com



From daemon Mon Jan 24 21:02:20 2000



From: Robert Derby :      rjderby-at-excite.com
Date: Mon, 24 Jan 2000 20:46:41 -0600
Subject: CCD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone or any Company know of a CCD that will do single photon
detection at 852 wavelength?
This is for a very specialized app.
Thanks in advance




************************************************
Robert J. Derby
New Mexico Institute of Technology
Socorro, N.M.
Phone - 505-835-5866
E-mail - rjderby-at-excite.com
************************************************





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com




From daemon Tue Jan 25 07:33:19 2000



From: Michael Reiner :      michael.reiner-at-Smail.Uni-Koeln.de
Date: Tue, 25 Jan 2000 07:52:03 +0100
Subject: EM: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members of the list,

first, I would like to wish you all the best for the new year.
Now my question:
Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?

Is it that delicate as LR-White (Meanwhile I don«t use LR-W older than
half a year). My bottle which was not opened many times, could be
roundabout three years old.

Thanks a lot,
Michael

Michael Reiner
Department of Anatomy I
University of Cologne
Germany
michael.reiner-at-smail.uni-koeln.de



From daemon Tue Jan 25 18:19:12 2000



From: Schryvers Dominique :      schryver-at-ruca.ua.ac.be
Date: Tue, 25 Jan 00 15:50:33 +0100
Subject: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,

we're starting a project of HRTEM on small (nanoscale) colouring metal
particles in glass. Does anyone have experience with sample preparation
for this type of material. We prefer ion-milling as this retains the
relative positions of the particles with respect to the surface. Any
suggestions, also on literature, are welcome.

Many thanks in advance,

Nick Schryvers




*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
*=* *=*
*=* Dr. D. Schryvers *=*
*=* Electron Microscopy for Materials Research (EMAT) *=*
*=* University of Antwerp, RUCA *=*
*=* Groenenborgerlaan 171 *=*
*=* B-2020 ANTWERP *=*
*=* Belgium *=*
*=* tel: 32-3-2180247 *=*
*=* fax: 32-3-2180257 *=*
*=* e-mail: schryver-at-ruca.ua.ac.be *=*
*=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
*=* *=*
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*



From daemon Tue Jan 25 18:19:12 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 25 Jan 2000 09:50:51 -0500
Subject: Re: cryo bio

Contents Retrieved from Microscopy Listserver Archives
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by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id IAA06203
for dist-Microscopy; Tue, 25 Jan 2000 08:53:13 -0600 (CST)
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for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 25 Jan 2000 08:52:42 -0600 (CST)
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Received: from empc1 by snarl.biotech.ufl.edu (8.8.5/4.09)
id JAA02445; Tue, 25 Jan 2000 09:47:10 -0500 (EST)
Message-Id: {4.2.0.58.20000125094650.00a545f0-at-biotech}
X-Sender: gwe-at-biotech
X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58


Some people have trouble and some do not. It is an Acrobat . After you
get the blank screen, try hitting the reload button on your browser
menu. This has worked for some people. I need to consult a web expert to
see why my PDF files cause trouble.

Nestor, are you out there?????

If you still have trouble, let me know and I will put it into an HTML file.
My apologies to anyone having trouble.

Greg Erdos


At 09:39 AM 01/25/2000 -0500, you wrote:
} Dear Greg;
} I was most interested to look at your tips etc. for cryo sectioning,
} but when I clicked on the snowflake, all I ended up with was a blank
} screen. Any idea what I did wrong (or is my computer system to blame?)
}
} thanks in advance
} shea
}
}
}
} Dr. S. Shea Miller
} Agriculture and Agri-Food Canada
} Eastern Cereal and Oilseed Research Centre
} 2068 K.W. Neatby Bldg
} Central Experimental Farm
} Ottawa, Ontario
} Canada K1A 0C6
} email: millers-at-em.agr.ca
} phone: 613-759-1760
} fax: 613-759-1701

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Tue Jan 25 18:19:29 2000



From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Tue, 25 Jan 2000 11:42:08 -0500
Subject: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Dominique,

Your best source of advice would be Scott Walck, at these contacts:

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)

Scott has been in the 'TEM of glass' business for years and has developed a
series of techniques relevant to the preparation of cross-sections of this
material, which I assume you require when you say, "we prefer ion-milling as
this retains the
relative positions of the particles with respect to the surface." As Scott
will probably tell you, there is a small-angle cleaving technique that you
may find preferable to ion milling.

Cheers
John

John P. McCaffrey
National Research Council of Canada
M-50, Montreal Rd.
Ottawa, Ontario
K1A 0R6 CANADA

tel: +613-993-7823
fax: +613-990-0202
email: john.mccaffrey-at-nrc.ca


-----Original Message-----
} From: Schryvers Dominique [mailto:schryver-at-ruca.ua.ac.be]
Sent: Tuesday, January 25, 2000 9:51 AM
To: Microscopy MAIL


Dear all,

we're starting a project of HRTEM on small (nanoscale) colouring metal
particles in glass. Does anyone have experience with sample preparation
for this type of material. We prefer ion-milling as this retains the
relative positions of the particles with respect to the surface. Any
suggestions, also on literature, are welcome.

Many thanks in advance,

Nick Schryvers




*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
*=* *=*
*=* Dr. D. Schryvers *=*
*=* Electron Microscopy for Materials Research (EMAT) *=*
*=* University of Antwerp, RUCA *=*
*=* Groenenborgerlaan 171 *=*
*=* B-2020 ANTWERP *=*
*=* Belgium *=*
*=* tel: 32-3-2180247 *=*
*=* fax: 32-3-2180257 *=*
*=* e-mail: schryver-at-ruca.ua.ac.be *=*
*=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
*=* *=*
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*



From daemon Tue Jan 25 18:19:59 2000



From: Yali Tang :      ytang-at-ameslab.gov
Date: Tue, 25 Jan 2000 17:53:48 +0100
Subject: BG by OM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear all,

we're starting a project of patterning on BG of YBCO films. I got
difficulties to find the BG by using opital microscope, because we
can not etch the sample before patterning. Does anyone have
experience with checking the GB by OM? We appraciate any suggestions
or references.

Thanks in advance,

Yali Tang
--



From daemon Tue Jan 25 18:19:36 2000



From: Mark Aindow :      maindow-at-ims.uconn.edu
Date: Tue, 25 Jan 2000 12:14:09 -0500
Subject: Postdoc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


University of Connecticut
Institute for Materials Science

Postdoctoral Research Position in Electron Microscopy

The Institute for Materials Science (IMS) at UConn is an
interdisciplinary center with the threefold mission of fostering
education, research and outreach in all areas of the materials
sciences. Due to a re-organization of the IMS Microscopy
Unit, a Postdoctoral Position has become available in the area
of transmission electron microscopy. The appointee will be
involved in a range of academic and industrial projects, and
will assist in developing the TEM facilities. Candidates should
hold a PhD in Materials Science, Physics or a related discipline
and must have extensive hands-on experience in a broad range
of electron microscopy techniques. Experience in instrument
development and/or computer image processing/simulation
would be beneficial. The appointment is for one year in the
first instance and is available immediately. Screening of the
applications will begin immediately and will continue until the
post is filled. Applications from under-represented groups,
including minorities, women and people with disabilities are
encouraged.

Interested candidates should send a curriculum vitae, including
publication list, and the names of at least three referees with
postal addresses, telephone numbers and Email addresses to:
Prof. M. Aindow, Institute for Materials Science, University of
Connecticut, 97 North Eagleville Road, U-3136, Storrs,
CT 06269-3136 USA. Email: maindow-at-ims.uconn.edu




From daemon Tue Jan 25 18:19:41 2000



From: Neilly,Joseph :      joe.p.neilly-at-abbott.com
Date: Tue, 25 Jan 2000 13:20:09 -0600
Subject: Mattel QX3 on the Mac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently asked Michael Davidson of FSU (http://microscopy.fsu.edu/) if he
had any success running the QX3 on a Mac w/ USB and he said "It works with a
windows 98 emulator, but very very slowly."

Several people have had success running the QX3 with twain drivers from other
programs such as Paint Shop Pro and Photoshop
(http://clubs.yahoo.com/clubs/qx3). Does anybody know of a twain driver that
would run the QX3 from a Mac?

Joe Neilly
MMMS Educational Outreach Chair
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com



From daemon Tue Jan 25 18:19:42 2000



From: Mitch McCartney :      Mitch.McCartney-at-alconlabs.com
Date: Tue, 25 Jan 2000 13:30:47 -0600
Subject: EM Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Title: (Technical-Level) Scientist-Electron Microscopy

Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle,
S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the
global leader in the discovery, development, manufacture and marketing of
ophthalmic pharmaceuticals and medical devices. Alcon expects to double its
sales over the next five years and has achieved a profit growth rate of
approximately 11% over the last several years. Historically, the company
commits 10% of sales to research and development. Products developed in the
last ten years generate 50% of current sales. The current product pipeline
is strong. Alcon was just renamed to the Fortune List of the 100 Best
Companies to Work for in America.

Location: Fort Worth was recognized by USA Today as one of the 20 best
cities in which to live and work.

Position Responsibilities: The EM Unit is a core resource for R&D, witnessed
by the fact that the staff of three generated 9,350 electron micrographs
from 1,160 processed specimens in 1998 alone. The successful candidate will
be a key member of the EM Unit who processes, examines and provides
preliminary interpretation of ophthalmic devices as well as human and animal
tissue specimens from a wide variety of R&D groups. S/he will provide
electron microscopic research and method development directed towards the
discovery of new drug candidates and unique ophthalmic devices, the
understanding of pathogenic mechanisms and the identification of new
therapeutic agents. S/he will handle the EM Unit commitment to several
groups: Surgical-Intraocular Lens, Toxicology, and Degenerative Diseases, as
well as contributing to Glaucoma Therapeutic Research, Surgical,
Formulations, Consumer Technical Support, Physical Characterization and
Pharmacokinetics.

Responsibilities include preparing ophthalmic devices for SEM and x-ray
analysis and human and animal tissue for TEM and SEM; use and daily
maintenance of Zeiss CEM-902, Cambridge Stereoscan-120 and Zeiss DSM-940,
and PGT System 4+ x-ray system; developing and implementing EM techniques
for various research projects; assisting with human and animal tissue
procedures; and providing preliminary interpretation of EM data.

Preferred Qualifications: Candidates should possess a Bachelor of Science
degree in a related discipline plus at least seven years of significant EM
experience related exclusively to human and animal tissue. Collaborative
and problem solving skills are essential for this position. The successful
candidate will also demonstrate highly refined interpersonal and technical
writing skills. Certification by or eligibility to be certified by the MSA
is a plus.

Alcon professionals enjoy state-of-the-art facilities in a year-round
business casual environment. Our company offers competitive salaries and a
wide array of excellent benefits: a very generous retirement plan and
dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life,
and accident insurance, death, dismemberment, illness and disability
benefits, tuition reimbursement, employee credit union, adoption assistance,
dependent care, and wellness programs, on-site fitness center, running
track, cafeteria, and company store, innovative paid time off and holidays,
and retiree medical coverage.

An Equal Opportunity and Affirmative Action Employer. Pre-employment drug
testing.

Please email your resume and salary requirements to:
Job28_1261-at-careers.alconlabs.com
Reference Code: EM




From daemon Tue Jan 25 18:19:43 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Tue, 25 Jan 2000 14:43:14 -0500
Subject: Subject: Bio Cryo/pdf file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Apparently several were unable to read the cryostat technique pdf file that
Dr. Greg Erdos had generously posted at his website. The answer to the
problem could be the version of Acrobat used. I had the "blank page"
problem with version 3.0 but no problem at all with version 4.0 (Mac).



*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Tue Jan 25 18:19:46 2000



From: wft03-at-health.state.ny.us
Date: Tue, 25 Jan 2000 15:14:36 -0500
Subject: Cold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!


Dear Lee,
Fair is fair. Often wind and rain at slightly above freezing is more
miserable
than snow at a few degrees below--having been both in San Francisco for the
first
condition and Albany NY for the second, I can say this with first-hand
authority.
Yours,
Bill Tivol




From daemon Tue Jan 25 18:19:49 2000



From: wft03-at-health.state.ny.us
Date: Tue, 25 Jan 2000 16:03:37 -0500
Subject: Repair/overhaul of a Kevex Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Appropriate vendors,
I have a Kevex detector which has been giving peak widths
larger than specs. It probably just needs an overhaul, but there may
be some repair necessary for the pre-amp and FET. Could anyone
who can undertake this please respond to me off-list with estimates
for various contingencies? TIA.
Yours,
Bill Tivol




From daemon Tue Jan 25 18:19:54 2000



From: Ronald C. Decker :      decker-at-utcdayton.com
Date: Tue, 25 Jan 2000 17:42:35 -0500
Subject: TEM & Aerospace lubrication - Researcher Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Our Ohio company is seeking an engineer / scientist to research the
relationships between the chemistry and microstructure of solid lubricant
and hard coatings and their performance in the lubrication of aerospace
systems. Research will involve a variety of surface analytical tools (XPS,
Raman, etc.) so that fundamental mechanisms of lubrication can be
elucidated. Emphasis on microstructure will require expertise with TEM and
SEM including preparation of SEM & TEM specimens of thin films and wear
scars on steel and ceramic substrates. Research will also involve
correlating thin film properties with deposition plasma characteristics and
making recommendations for improving lifetime and performance of such
materials in different environments: e.g., vacuum, moist air, high
temperature, etc.

It is important that candidates have capabilities in cross-section TEM,
analytical TEM, analysis of unique microstructures; and understand TEM of
thin films on a fundamental level. It is desirable that the candidate have
knowledge of tribological materials and experience with TEM/XTEM of wear
tracks.

Contact Ronald Decker - mailto:decker-at-utcdayton.com

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Ronald C. Decker
Program Manager
Universal Technology Corporation
1270 N FAIRFIELD RD
DAYTON OH 45432-2600

Voice (937) 426-8530, Fax (937) 426-7753
(Voice mail is available at my extension, 270)

http://www.utcdayton.com/
mailto:decker-at-utcdayton.com
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}




From daemon Tue Jan 25 18:19:54 2000



From: Pete :      pjpns-at-worldnet.att
Date: Tue, 25 Jan 2000 17:51:25 -0500
Subject: Spectrophotometer Schematics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Schematics for Carl Zeiss Spectrophotometer DM4, DMR21, PMQII being discarded. Please
respond by 1/31/00 if interested.

Pete Dondl
pjpns-at-worldnet.att.net



From daemon Tue Jan 25 18:19:58 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Tue, 25 Jan 2000 15:28:42 -0800 (PST)
Subject: Re: Mattel QX3 on the Mac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


While browsing news from the latest MacWorld Expo I came across a brief
comment about a USB video microscope for the Mac. Further searches at the
MacWorld Expo website or at Apple's web site have not turned up anything
more about it, although there were announcements that Data Translation and
National instruments
have released additional PCI and USB I/O boards for the Mac. Has anyone
heard more of this?


Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu





From daemon Tue Jan 25 19:30:11 2000



From: Snow, David B. :      snowdb-at-pweh.com
Date: Tue, 25 Jan 2000 19:17:31 -0600
Subject: Job Opening:High Resolution Scanning Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




High Resolution Scanning Electron Microscopist/Engineer


United Technologies Research Center is seeking an engineer to fill the
HR-SEM operator/engineer position at the United Technologies Research
Center in East Hartford, CT. This position will provide support to the
United Technologies Corporation Business Unites including Pratt & Whitney,
Carrier, Sikorsky, Hamilton Sundstrand and Otis. The SEM operator/engineer
will be responsible for the full utilization of both high-resolution
secondary and back-scattered imaging to characterize a wide range of
metallic and non-metallic materials, including surface coatings and
advanced structural materials including metals and ceramics. In addition,
the ability to recognize fracture modes and origins of fractures is
strongly desired. The candidate should be experienced in the use of EDS
for both qualitative and quantitative analyses, including compositional
mapping and line profiles. The qualified candidate must be capable of
judging the optimal combinations of imaging and EDS to yield t!
he most informative characterization of a particular specimen. Good
communication and interpersonal skills are essential. Experience with
electron backscatter diffraction (EBSD) is a plus.

Qualified candidates will have BS in Materials Science or an equivalent
discipline, with a minimum of 2 years SEM experience. U.S. citizenship or
permanent resident status is required.

Please visit our web site at http://www.utrc.utc.com for additional general
information. Interested parties should send a letter of application and a
resume to Employment Opportunities, Code MATS-2050-9049, United
Technologies Research Center, 411 Silver Lane, East Hartford, CT 06108 or
e-mail employment-at-utrc.utc.com. United Technologies Research Center is an
equal opportunity employer.




From daemon Wed Jan 26 08:08:20 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Tue, 25 Jan 2000 20:13:24 -0600
Subject: Re: Subject: Bio Cryo/pdf file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I tried to open the file with Acrobat 4.0 in Windows 98. No luck.

Damian Neuberger
etc., etc.



} Apparently several were unable to read the cryostat technique pdf file that
} Dr. Greg Erdos had generously posted at his website. The answer to the
} problem could be the version of Acrobat used. I had the "blank page"
} problem with version 3.0 but no problem at all with version 4.0 (Mac).



From daemon Wed Jan 26 08:08:25 2000



From: Bill Carmichael :      billc-at-jvlnet.com
Date: Tue, 25 Jan 2000 21:58:42 -0600
Subject: ISI SS40 parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

We have an ISI SS40 SEM that is in need of a discontinued part. It is a
NEC transistor, number D588. It is used in the filament current
control circuit. I'm having trouble locating the part because it has
not been manufactured by NEC since 1984. Does anyone know of a source
for this part or a substitute transistor?

Thanks in advance,

Bill Carmichael

______________________________________
Bill Carmichael
Electron Microscopy Faculty
Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309
wcarmichael-at-madison.tec.wi.us

http://electron-microscopy.madison.tec.wi.us




From daemon Wed Jan 26 08:08:25 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Tue, 25 Jan 2000 20:11:00 -0800
Subject: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dominique,
Glass is readily microtomed with a diamond knife and may be a suitable
inexpensive technique for you to consider. Particularly if the materials
of your sample are sufficiently dissimilar in reaction to the chemical and
ion etching of some techniques. I've been embedding and sectioning coated
glass, optics, and other hard materials for 18 years (even diamond coated
silicon in Microscopy Research and Technique, vol. 31, p. 308, 1995). The
imaging and analysis of nano-structures in micron sized areas near the
surface of glass is routine, fast, and inexpensive for physical
microstructure and chemistry. Mechanical artifacts generated in
ultramicrotomy tend to be quite large, readily visible, and easily ignored
but may interfere with the analysis of naturally occurring deformation
features (i.e. twinning, slip, etc.). Any good diamond knife will work
with meticulous and careful technique, but experience has shown that 35
degree knives yield the best results with hard and ultra-hard materials.

The critical elements for microtomy of hard, non-porous materials include:
1. Minimize the cross-sectional area to be sectioned. An easy way is to do
this is to pop concoidal micro-chips from the surface. These tend to be
very thin at the edges and may be further broken to form very pointed thin
samples. [Time = ~20 minutes]
2. Optimize sample orientation for sectioning and preferred orientation.
Some physical microstructures are anisotropic and are difficult to
interpret when viewed in the wrong orientation. [Time = ~10 minutes]
3. Maximize adhesion to the resin through the selection of an appropriate
resin (low viscosity and non-reactive with your sample), meticulous and
contamination-free sample prep, and the addition of adhesion promoters
(such as Dow Corning Z-6040). [Time = ~1 hour, with an over night epoxy
cure]
4. Section using standard procedures, but minimize the sectioning speed
(optimize cutting speed). [Time = ~1 hour]

These times are approximate for 1 sample, and there could be economy in
numbers. As always, each case will require individual attention.
Cheers,

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Schryvers Dominique [SMTP:schryver-at-ruca.ua.ac.be]
Sent: Tuesday, January 25, 2000 6:51 AM
To: Microscopy MAIL


Dear all,

we're starting a project of HRTEM on small (nanoscale) colouring metal
particles in glass. Does anyone have experience with sample preparation
for this type of material. We prefer ion-milling as this retains the
relative positions of the particles with respect to the surface. Any
suggestions, also on literature, are welcome.

Many thanks in advance,

Nick Schryvers




*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
*=* *=*
*=* Dr. D. Schryvers *=*
*=* Electron Microscopy for Materials Research (EMAT) *=*
*=* University of Antwerp, RUCA *=*
*=* Groenenborgerlaan 171 *=*
*=* B-2020 ANTWERP *=*
*=* Belgium *=*
*=* tel: 32-3-2180247 *=*
*=* fax: 32-3-2180257 *=*
*=* e-mail: schryver-at-ruca.ua.ac.be *=*
*=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
*=* *=*
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*





From daemon Wed Jan 26 08:08:57 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Wed, 26 Jan 2000 09:42:35 +0100
Subject: Re: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nick,

we are analyzing frequently this kind of specimens. In our case the
particles are silver. Depending on density and size distribution they are
changing the color of the glass.
Since we are interested in the depth distribution starting at the interface
with a silver containging layer on the glass, we need to do a
cross-sectional preparation. Therefore, we use several steps of preparation
as described below:

á gluing of two coated glass surfaces face to face

á cutting and polishing of the obtained block to a size of 2 x 1 x 10 mmÒ
with the interface plane running parallel to the 2 mm x 10 mm face and in
the middle of the block

á gluing of this block in a steel-cylinder of 3 mm diameter and 10 mm
length with a 1 mm x 2 mm rectangular hole along the cylinder axis

á cutting of 0.5 - 1 mm thick slices perpendicular to the cylinder axis

á fine polishing of one face of the obtained disk (lowest grain size: 1µm)

á grinding of the disk from the opposite face down to 100 µm thickness with
subsequent fine polishing

á dimpling of a crater into one face of the disk with a residual thickness
in the middle of the disk of about 10 µm

á ion etching of the flat side of the sample with argon ions of 4 kV under
an angle of 2 - 4 degrees with a current of about 12 µA until electron
transparency is reached in the region of the interface.

Some of the results were presented on the FEMMS-Meeting in Irsee, Germany,
1998.
For more details, you might contact me directly.

Hope this helps,

Petra

At 15:50 25.01.00 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Jan 26 08:28:08 2000



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Wed, 26 Jan 2000 14:00:54 -0000
Subject: SEM - Halophilic bacteria preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anybody offer a method for the preparation of Halophilic bacteria for
'standard' SEM observation? post fixation washing appears to produce cell
lysis!.



From daemon Wed Jan 26 08:28:09 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 26 Jan 2000 09:19:58 -0500
Subject: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For those who are unable to view the PDF file of Cryostat information that
I posted, I have also posted it in ugly HTML. I am still trying to find
out why some can read the PDF and others cannot. The Version of Acrobat
does not seem to be the answer.

My apologies to anyone who got frustrated.
Once again the site is:
www.biotech.ufl.edu/sems/

Greg Erdos
Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Wed Jan 26 09:29:37 2000



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Wed, 26 Jan 2000 09:39:51 -0500
Subject: No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please keep weather reports private.



} } } {"wft03-at-health.state.ny.us"-at-sparc5.Microscopy.Com} 01/25 3:14 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America






}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!


Dear Lee,
Fair is fair. Often wind and rain at slightly above freezing is more
miserable
than snow at a few degrees below--having been both in San Francisco for the
first
condition and Albany NY for the second, I can say this with first-hand
authority.
Yours,
Bill Tivol






From daemon Wed Jan 26 09:29:37 2000



From: Ann-Fook Yang (Ann-Fook Yang) (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Wed, 26 Jan 2000 09:43:30 -0500
Subject: No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please keep weather reports private.

Ann Fook



} } } {"wft03-at-health.state.ny.us"-at-sparc5.Microscopy.Com} 01/25 3:14 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America






}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!


Dear Lee,
Fair is fair. Often wind and rain at slightly above freezing is more
miserable
than snow at a few degrees below--having been both in San Francisco for the
first
condition and Albany NY for the second, I can say this with first-hand
authority.
Yours,
Bill Tivol






From daemon Wed Jan 26 19:00:50 2000



From: Peter Guthrie :      Peter.Guthrie-at-hsc.utah.edu
Date: Wed, 26 Jan 2000 09:28:03 -0700 (MST)
Subject: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It would be convenient to be able to bring a video camera into an
elementary school classroom, hook it up to a microscope (with an eyepiece
adaptor) and display the microscope image for an entire classroom to see.
If there is a video monitor (or a TV with a video input), this is fairly
easy. If there is not an available monitor, I should be able to bring in a
laptop and display the image on the laptop screen.

I am looking for an inexpensive lightweight video camera (with a C-mount)
with either a firewire or USB linkage.

Does anyone have any suggestions?

Thanks

Peter Guthrie
Department of Neurobiology & Anatomy
University of Utah School of Medicine
50 N Medical Drive
Salt Lake City, UT 84132
(801) 581-8336 (801) 581-4233 fax
Peter.Guthrie-at-hsc.utah.edu




From daemon Wed Jan 26 19:00:51 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 26 Jan 2000 08:39:59 -0800
Subject: Re: Repair/overhaul of a Kevex Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DearBill,
When my Kevex detector showed degraded resolution, I turned off the bias and
grounded the BNC plug with a paper clip, then warmed it up completely to get
rid of ice and frost in the detector. This brought back my resolution, but
degraded my LN2 holding time. Then I had a friend in Physics pump out the
dewar and now I'm back to peak performance.
04:03 PM 1/25/00 -0500, you wrote:
} Appropriate vendors,
} I have a Kevex detector which has been giving peak widths
} larger than specs. It probably just needs an overhaul, but there may
} be some repair necessary for the pre-amp and FET. Could anyone
} who can undertake this please respond to me off-list with estimates
} for various contingencies? TIA.
} Yours,
} Bill Tivol
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Jan 26 19:00:53 2000



From: Dan Kremser :      dkremser-at-levee.wustl.edu
Date: Wed, 26 Jan 2000 11:16:41 -0600
Subject: Biological Standards for Microprobe Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all:

Recently, an interesting medical school project has appeared at my lab
door. I've been asked to quantify Ca and P in scar tissue found in
sections of hamster hearts. Obtaining appropriate standards is critical.
Are there commercially available biological standards or procedures to
create such standards out there? Any suggestions would be appreciated and
thanks for your time.

Dan


=====================================================
Dan Kremser
Washington University
Department of Earth and Planetary Sciences/Campus Box 1169
(Wilson Hall, Room 108-----for packages)
One Brookings Dr.
St. Louis, MOÊ 63130-4899

VOICE: (314) 935-5605Ê FAX: (314) 935-7361
E-MAIL: dkremser-at-levee.wustl.edu



From daemon Wed Jan 26 19:00:55 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 26 Jan 2000 11:44:14 -0600
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How about using a device like Dazzle or Snappy to display the video stream
from your video camera on a laptop? It would require a little software
setup but should work. The models I am familiar with used parallel port
connections, but there ought to be models around that would use USB.

At 09:28 AM 1/26/2000 -0700, you wrote:
} It would be convenient to be able to bring a video camera into an
} elementary school classroom, hook it up to a microscope (with an eyepiece
} adaptor) and display the microscope image for an entire classroom to see.
} If there is a video monitor (or a TV with a video input), this is fairly
} easy. If there is not an available monitor, I should be able to bring in a
} laptop and display the image on the laptop screen.
}
} I am looking for an inexpensive lightweight video camera (with a C-mount)
} with either a firewire or USB linkage.
}
} Does anyone have any suggestions?
}
} Thanks



From daemon Wed Jan 26 19:00:54 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 26 Jan 2000 13:38:41 -0600
Subject: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Listservers,
Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details.
TIA
Hank Adams
Integrated Microscopy Core
Molecular and Cell Biology
Baylor College of Medicine
Houston, TX



From daemon Wed Jan 26 19:01:19 2000



From: A. K. Christensen :      akc-at-umich.edu
Date: Wed, 26 Jan 2000 17:15:30 -0500
Subject: Re: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hank,

Here are three good references:

Stefanini M, De Martino C, Zamboni L. 1967. Fixation of ejaculated
spermatozoa for electron microscopy. Nature 216:173-174.

Meschede D, Keck C, Zander M, Cooper TG, Yeung CH, Nieschlag E. 1993.
Influence of three different preparation techniques on the results of human
sperm morphology analysis. Int J Androl 16:362-369.

Phillips DM. 1995. Fixation of mammalian spermatozoa for electron
microscopy. In: Dentler W, Witman G (Eds.), Methods in Cell Biology, Vol
47, vol 47. Academic Press Inc (San Diego), pp 199-204.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Office: 2703A Medical Science II Building
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
E-mail: akc-at-umich.edu
http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Wed, Jan 26, 2000 1:38 PM -0600 hank adams {hpadams-at-bcm.tmc.edu} wrote:

} Listservers,
} Can anyone lead me to a good reference for processing human sperm for
} TEM? Or if you have a procedure can you please forward the details. TIA
} Hank Adams
} Integrated Microscopy Core
} Molecular and Cell Biology
} Baylor College of Medicine
} Houston, TX




From daemon Wed Jan 26 19:01:19 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Wed, 26 Jan 2000 17:13:00 -0600
Subject: Re:USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,

If you intend to use a "video" camera, you may need a capture card rather
than,
or in addition to, a USB or Firewire interface. Most video cams are analog
(NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly
have digital circuits internal (like the new digital camcorders or the
PCcams
used for video conferencing on the net). ...You first need to determine
the
source format.

Warren's suggestion implements an inexpensive external NTSC* video frame
grabber
(Snappy). Which will "grab" an analog video frame and digitize it. *May do
PAL
too??

Parallel port I/O is a bit slow (or is that a byte slow :) for pictures
containing lots of data, but is cheap and works since the inherent
resolution of
typical NTSC video is { 640x480. USB is very much faster and Firewire
faster
yet (and usually a lot more $$ for the interface card). For applications
other
than full frame rate/resolution streaming video, USB is fine.

Woody White



From daemon Wed Jan 26 19:16:54 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Wed, 26 Jan 2000 20:04:33 -0500
Subject: Re: USB or firewire cameras

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Peter,

Take a look at this site....

http://www.dazzlemultimedia.com/html/products/index.htm

They have a USB version and the software interface is great. It is easy to use
and has a street price of about $180!

Good Luck,

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland
nikon-at-jagunet.com

***I have no affiliation with Dazzle, Inc.***


"White, Woody N" wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Peter,
}
} If you intend to use a "video" camera, you may need a capture card rather
} than,
} or in addition to, a USB or Firewire interface. Most video cams are analog
} (NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly
} have digital circuits internal (like the new digital camcorders or the
} PCcams
} used for video conferencing on the net). ...You first need to determine
} the
} source format.
}
} Warren's suggestion implements an inexpensive external NTSC* video frame
} grabber
} (Snappy). Which will "grab" an analog video frame and digitize it. *May do
} PAL
} too??
}
} Parallel port I/O is a bit slow (or is that a byte slow :) for pictures
} containing lots of data, but is cheap and works since the inherent
} resolution of
} typical NTSC video is { 640x480. USB is very much faster and Firewire
} faster
} yet (and usually a lot more $$ for the interface card). For applications
} other
} than full frame rate/resolution streaming video, USB is fine.
}
} Woody White



From daemon Wed Jan 26 23:12:23 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 26 Jan 00 17:52:26 -0800
Subject: Re:No weather report please

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} Please keep weather reports private.
}
} Ann Fook

Why? A little light heartedness never hurt anyone.
For the record, LA was sunny as usual today. Happy I don't live in CT anymore.

Regards,

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Jan 26 23:12:28 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Wed, 26 Jan 2000 20:39:59 -0600
Subject: Re: Firewire Video & no need for capture board

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I currently video with a Sony DV Video camera, fire wire connect to
my computer with no capture board.

It does require special software however, We bought Final Cut Pro,
But from trying out the demo and reading, It appears Adobe Priemier
also will allow firewire capture without a board. Though Adobe's is
one I have not tried, I'd call first.

This works fine for video, and I can pull off individual frames for
low res images for the web, and ok small images to print if very
small.

But, in emailing to and from Sony, It was my understanding that if I
were to firewire images, I WOULD need a board.

My video camera will do both images and video. FinalCut Pro pulls
off the video, but I can't seem to get it to recognize the individual
image shots. So I believe Sony, though I may just not have selected
the right buttons etc.

If pulling in video by fire wire, especially pulling it onto a
firewire hard drive ( ie VST) It is pretty close to real time video.

Lou Ann
***************************
Lou Ann Miller
Service Supervisor
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://treefrog.cvm.uiuc.edu

Central States Microscopy Society
http://treefrog.cvm.uiuc.edu/csmms

Personal Home Page:
http://treefrog.cvm.uiuc.edu/lam



From daemon Wed Jan 26 23:12:33 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 27 Jan 2000 13:38:03 +1000
Subject: RE: SEM - Halophilic bacteria preparation

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I did some TEM work on the extreme holophile bacterias (they grow in saturated
NaCl solutions) some 30 years ago. Very difficult specimens, it seems no
fixation is complete and can prevent osmotic shock.

I have not tried SEM on halophiles, but I suggest this:
You could try excessive fixation, using 2 hours at room 20 degrees.
I would use a several molar solution of ammonium acetate to rinse the specimen
after fixation. Ammonium acetate is a volatile salt solution and leaves no
crystals after evaporation.
The still wet sample (mounted on a 10mm coverslip) could then be placed in a
glass Petrie dish which has a double layer of filter paper, saturated with
chloroform.
Place the closed Petrie dish in the fridge for a day or two. Warm the dish
before opening (to avoid condensation) and metal coat prior to SEM.

Ah, your first problem could be the fixation. The osmium (I'd forget GA), would
need to go into the bacteria's growth medium, or use vapour fixation only. Even
then, I expect much damage before any further processing.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 27, 2000 12:01 AM, Hyman, S.C.
[SMTP:sch10-at-leicester.ac.uk] wrote:
}
}
} Can anybody offer a method for the preparation of Halophilic bacteria for
} 'standard' SEM observation? post fixation washing appears to produce cell
} lysis!.



From daemon Wed Jan 26 23:12:32 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 27 Jan 2000 00:31:22 -0800
Subject: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {388FBBF2.61FF-at-mail.superlink.net}


Message-ID: {388FBBF2.61FF-at-mail.superlink.net}


Moreover, it is well known that weather may affect the quality of EM
samples. For instance, humidity is very critical for many EM techniques. I
utilized very unusual technique for holey-film preparation with
calcium-rhodanide. This technique is extremely sensitive for
humidity/temperature combination. When I was working in Russia (without
conditioner in the room), I was able predict the weather changes using that
technique. Again, it was tricky to work when temperature in the room was
around 7oC (at winter). The guys from East Coast may have something like
that. Why not to share experience how to work at different weather
conditions?

Have a good weather!

Sergey

} Date: Wed, 26 Jan 2000 17:52:26 -0800
} From: Paul Webster {pwebster-at-hei.org}
} Subject: Re:No weather report please
} To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com}
} Reply-to: Paul Webster {pwebster-at-hei.org}
} X-Mailer: QuickMail Pro 1.5.4 (Mac)
} X-MIME-Autoconverted: from quoted-printable to 8bit by
ultra5.microscopy.com id
} TAA11085
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Thu Jan 27 06:51:48 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 27 Jan 2000 00:57:54 -0800 (PST)
Subject: Re: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hank and others,
I have had good success this Stefanini's buffered picric acid
paraformaldehyde (PAF) for spermatozoan. I do not have the fixative
formula at my fingertips, but the reference is: Nature Vol. 216, OCT 14.
1967.

I will look it up if you are interested and get back to you or you can
email me-at- tiekotte-at-up.edu.
-Ken

Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N Willamette Blvd.
Portland, OR 97303



On Wed, 26 Jan 2000, hank adams wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listservers,
} Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details.
} TIA
} Hank Adams
} Integrated Microscopy Core
} Molecular and Cell Biology
} Baylor College of Medicine
} Houston, TX
}
}



From daemon Thu Jan 27 06:51:58 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Thu, 27 Jan 2000 11:36:12 +0100
Subject: Re: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Erdos,

Perheps it is due to the difference between Netscape and IE. I got a blank
page with netscape but read it correctly with IE4.0.

Shu-You Li
**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************



} From: Greg Erdos {gwe-at-biotech.ufl.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Cryostat info.
} Date: Wed, 26 Jan 2000 09:19:58 -0500
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} For those who are unable to view the PDF file of Cryostat information that
} I posted, I have also posted it in ugly HTML. I am still trying to find
} out why some can read the PDF and others cannot. The Version of Acrobat
} does not seem to be the answer.
}
} My apologies to anyone who got frustrated.
} Once again the site is:
} www.biotech.ufl.edu/sems/
}
} Greg Erdos
} Gregory W. Erdos, Ph.D. Ph.
} 352-392-1295
} Assistant Director, Biotechnology Program
} PO Box 110580 Fax:
} 352-846-0251
} University of Florida
} Gainesville, FL 32611
}



From daemon Thu Jan 27 06:52:00 2000



From: Tony Bruton :      bruton-at-EMU.UNP.AC.ZA
Date: Thu, 27 Jan 2000 14:29:04 +0200
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
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Listers

I think that we are proceeding slightly tongue-in-cheek here - but to continue the 'serious' note;

The most weather sensitive task I have ever tackled is the production of formvar films. With the extremely high humidities we experience here there are times when the formvar will simply NOT separate from the substrate, despite 'air-conditioning'.

Tony



Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website via:http:www.nu.ac.za
Email:bruton-at-emu.unp.ac.za
postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } Sergey Ryazantsev {sryazant-at-ucla.edu} 01/27/00 10:31AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Moreover, it is well known that weather may affect the quality of EM
samples. For instance, humidity is very critical for many EM techniques. I
utilized very unusual technique for holey-film preparation with
calcium-rhodanide. This technique is extremely sensitive for
humidity/temperature combination. When I was working in Russia (without
conditioner in the room), I was able predict the weather changes using that
technique. Again, it was tricky to work when temperature in the room was
around 7oC (at winter). The guys from East Coast may have something like
that. Why not to share experience how to work at different weather
conditions?

Have a good weather!

Sergey

} Date: Wed, 26 Jan 2000 17:52:26 -0800
} From: Paul Webster {pwebster-at-hei.org}
} Subject: Re:No weather report please
} To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com}
} Reply-to: Paul Webster {pwebster-at-hei.org}
} X-Mailer: QuickMail Pro 1.5.4 (Mac)
} X-MIME-Autoconverted: from quoted-printable to 8bit by
ultra5.microscopy.com id
} TAA11085
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant






From daemon Thu Jan 27 07:12:03 2000



From: Rick Powell at Nanoprobes :      rpowell-at-lihti.org
Date: Thu, 27 Jan 2000 08:02:45 -0500
Subject: Light microscopy:Fluorogold suppliers

Contents Retrieved from Microscopy Listserver Archives
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Hello Light Microscopists:

Can anyone tell me who currently makes and sells the retrograde neuronal
tracer, Fluorogold? We have a customer who is confusing it with our
FluoroNanogold products (not the first time this has happenned) and I would
lkke to point them to the right source!

Thanks,

Rick Powell


**********************************************************************
* NANOPROBES, Incorporated | US Toll-free: (877) 808-2101 *
* 25 East Loop Road, Suite 113 | Tel: (919) 510-0590 *
* Stony Brook, NY 11790-3350, | Fax: (919) 510-0590 *
* USA | rpowell-at-lihti.org *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************




From daemon Thu Jan 27 07:36:36 2000



From: Robinson John :      emxray-at-server.uwindsor.ca
Date: Thu, 27 Jan 2000 08:09:45 -0500
Subject: Test

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Jan 27 07:41:58 2000



From: RAHBARI, RAMIN :      RAMIN.RAHBARI-at-WL.com
Date: Thu, 27 Jan 2000 09:29:54 -0500
Subject: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Some time ago, Kent Christensen, Univ. of Michigan, (akc-at-umich.edu) kindly
supplied to me the following:


Zamboni's Fixative

FROM:rj.wilson-at-qut.edu.au (Russell J. Wilson)
The following is the composition of the Zamboni's Fixative

0.2M Na2HPO4.....................390mL
0.2M NaH2PO4.....................110mL
16% Paraformaldehyde.............25mL
Saturated Picric Acid............15mL
Distilled Water..................10mL
...........................................................................
.................

The main reference is: Stefanini et al., 1967, Nature 216:173.


Ramin Rahbari
PARKE-DAVIS Pharmaceutical Research
Worldwide Preclinical Safety
2800 Plymouth Road
Ann Arbor, MI 48105
Voice (734) 622-3383
Fax (734) 622-5001
Ramin.Rahbari-at-WL.COM


-----Original Message-----
} From: hank adams [mailto:hpadams-at-bcm.tmc.edu]
Sent: Wednesday, January 26, 2000 2:39 PM
To: 'microscopy-at-msa.microscopy.com'


Listservers,
Can anyone lead me to a good reference for processing human sperm
for TEM? Or if you have a procedure can you please forward the details.
TIA
Hank Adams
Integrated Microscopy Core
Molecular and Cell Biology
Baylor College of Medicine
Houston, TX



From daemon Thu Jan 27 17:57:49 2000



From: Sally Schroeter :      sally.schroeter-at-mcmail.vanderbilt.edu
Date: Thu, 27 Jan 2000 09:36:51 -0600
Subject: video to laptop display

Contents Retrieved from Microscopy Listserver Archives
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As far as I could discover, Dazzler and Snappy are for Windows
machines only. Are there similar devices available for Macintoshes?




From daemon Thu Jan 27 17:57:55 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Thu, 27 Jan 2000 11:29:14 -0500
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




If anyone remembers I use to end all my e-mails to the listees with a quite
sarcastic weather and/or olfactory report from the garden state. Either no one
read my posts or they just didn't get the East Coast thing.

Cold, windy and something smells real odd under the Pulaski Skyway...Aloha!

John Grazul
Lucent

Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Moreover, it is well known that weather may affect the quality of EM
} samples. For instance, humidity is very critical for many EM techniques. I
} utilized very unusual technique for holey-film preparation with
} calcium-rhodanide. This technique is extremely sensitive for
} humidity/temperature combination. When I was working in Russia (without
} conditioner in the room), I was able predict the weather changes using that
} technique. Again, it was tricky to work when temperature in the room was
} around 7oC (at winter). The guys from East Coast may have something like
} that. Why not to share experience how to work at different weather
} conditions?
}
} Have a good weather!
}
} Sergey
}
} } Date: Wed, 26 Jan 2000 17:52:26 -0800
} } From: Paul Webster {pwebster-at-hei.org}
} } Subject: Re:No weather report please
} } To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com}
} } Reply-to: Paul Webster {pwebster-at-hei.org}
} } X-Mailer: QuickMail Pro 1.5.4 (Mac)
} } X-MIME-Autoconverted: from quoted-printable to 8bit by
} ultra5.microscopy.com id
} } TAA11085
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } Please keep weather reports private.
} } }
} } } Ann Fook
} }
} } Why? A little light heartedness never hurt anyone.
} } For the record, LA was sunny as usual today. Happy I don't live in CT
} anymore.
} }
} } Regards,
} }
} } Paul Webster, Ph.D.
} } Associate Scientist & Director
} } Ahmanson Advanced Electron Microscopy & Imaging Center
} } House Ear Institute
} } 2100 West Third St.
} } Los Angeles, CA 90057
} }
} } Phone: (213) 273-8026
} } Fax: (213) 413-6739
} } e-mail: pwebster-at-hei.org
} } http://www.hei.org/htm/aemi.htm
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant



From daemon Thu Jan 27 17:57:55 2000



From: J.F. Bailey :      jfb-at-uidaho.edu
Date: Thu, 27 Jan 2000 08:34:11 -0800
Subject: ultramicrotome service

Contents Retrieved from Microscopy Listserver Archives
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Is anyone aware of a company who would service an old LKB Ultratome
III? If so, please contact me at: jfb-at-uidaho.edu

Thank you.



From daemon Thu Jan 27 17:57:57 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Thu, 27 Jan 2000 10:50:50 -0600
Subject: Re: Light microscopy:Fluorogold suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Molecular Probes sells hydroxystilbamidine, methanesulfonate (cat. # H-7599)
which I beleive is the active chemical in Fluorogold. (see paper by Martin W.
Wessendorf in Brain Res 553(1): 135-48. Jul 1991).

Karen Zaruba

P.S. I have no interest in Molecular Probes other than a satisfied customer.


Rick Powell at Nanoprobes wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Light Microscopists:
}
} Can anyone tell me who currently makes and sells the retrograde neuronal
} tracer, Fluorogold? We have a customer who is confusing it with our
} FluoroNanogold products (not the first time this has happenned) and I would
} lkke to point them to the right source!
}
} Thanks,
}
} Rick Powell
}
} **********************************************************************
} * NANOPROBES, Incorporated | US Toll-free: (877) 808-2101 *
} * 25 East Loop Road, Suite 113 | Tel: (919) 510-0590 *
} * Stony Brook, NY 11790-3350, | Fax: (919) 510-0590 *
} * USA | rpowell-at-lihti.org *
} * *
} * NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
} **********************************************************************

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Thu Jan 27 17:57:57 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 27 Jan 2000 09:07:18 -0800
Subject: Cynthia Shannon-Are you there?

Contents Retrieved from Microscopy Listserver Archives
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Tried to respond to a message posted here from Cynthia Shannon re: a used
TEM, it keeps bouncing. Cynthia, if you are out there, how can I contact
you?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Jan 27 17:57:58 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 27 Jan 2000 11:27:02 -0600
Subject: EM position opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ELECTRON MICROSCOPY TECHNICIAN
The Integrated Microscopy Core, Department of Molecular and Cell Biology,
Baylor College of Medicine is expanding and has an immediate full-time
opening for an electron microscopy technician. The Integrated Microscopy
Core is a growing, state-of-the-art facility with 2 TEMs, deconvolution, laser
scanning confocal, 2 CCD-based fluorescent microscopes and multiple PC and
Silicon Graphics workstations for imaging software. The applicant should
have at least one year of experience in various aspects of sample
preparation for biological TEM including fixation, embedding, ultrathin
sectioning and staining. The applicant should have darkroom experience and
experience in the operation of TEMs. Other duties include preparation of
solutions, embedding media and the maintaining of records. The position
offers excellent opportunities for training in advanced light and electron
microscopy techniques, including immunofluorescence and immunogold labeling,
laser scanning confocal and deconvolution microscopy, as well as live
imaging of GFP-tagged proteins. Training in several image computer-based
imaging programs will also be available (PhotoShop, MetaMorph, SoftWorks).
The position requires a minimum of a Bachelors degree and will start as a
Lab Technician II; salary will be commensurate with experience, and includes
the standard Baylor benefits package.

Send CV and letter of research/technical interests to:

Hank Adams
Laboratory Manager
Integrated Microscopy Core
Department of Molecular and Cell Biology
Baylor College of Medicine
One Baylor Plaza
Houston, TX 77030
Email submissions to: hpadams-at-bcm.tmc.edu
Fax submissions to: 713 790 0545

Baylor College of Medicine is an Equal Opportunity, Affirmative Action and
Equal Access Employer.



From daemon Thu Jan 27 17:58:00 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 27 Jan 2000 09:45:24 -0800 (PST)
Subject: Re: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can't see how it can be netscape vs IE, since I got the blank page using
IE4. I haven't tried netscape or IE5 (have both at home--but not here at
work). Acrobat seems to work on every other PDF file I have opened (the
intranet and internet standard here for public documents in a read-only
setting), so I don't think the version of Acrobat is the problem either. I
tried Dr. Erdos' original work-around, but still got the blank page.
????


On Thu, 27 Jan 2000 11:36:12 +0100, Shu-You Li wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Dr. Erdos,
}
} Perheps it is due to the difference between Netscape and IE. I got a
blank
} page with netscape but read it correctly with IE4.0.
}
} Shu-You Li
} **************************************************
} Shu-You Li, Dr.
} Institut fuer Physikalische Chemie
} Johannes Guttenberg Universitaet
} Jakob-Welder-Weg 11
} D-55099 Mainz, Germany
}
} E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
} Fax: +49-6131-3923768
} Tel: +49-6131-3923148(O)
} **************************************************
}
}
}
} } From: Greg Erdos {gwe-at-biotech.ufl.edu}
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Cryostat info.
} } Date: Wed, 26 Jan 2000 09:19:58 -0500
} }
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
} }
} }
} } For those who are unable to view the PDF file of Cryostat information
that
} } I posted, I have also posted it in ugly HTML. I am still trying to
find
} } out why some can read the PDF and others cannot. The Version of
Acrobat
} } does not seem to be the answer.
} }
} } My apologies to anyone who got frustrated.
} } Once again the site is:
} } www.biotech.ufl.edu/sems/
} }
} } Greg Erdos
} } Gregory W. Erdos, Ph.D. Ph.
} } 352-392-1295
} } Assistant Director, Biotechnology Program
} } PO Box 110580
Fax:
} } 352-846-0251
} } University of Florida
} } Gainesville, FL 32611
} }
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Thu Jan 27 17:58:00 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 27 Jan 2000 10:48:26 -0700
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think, there is some confusion out there about digital and analog
cameras and different protocols and interfaces. Woody's posting is
correct, but perhaps a look at some of the current implementations might
help:

1) Cameras come either with a digital output or analog output. As Woody
mentions, there are several different analog standards (PAL, NTSC,
RS-170,...) and formats for transmitting the data (RGB, S-VHS,
composite, ...)

2) Regardless of what the signal is, there must be some "device" that
translates the incoming signals into "numbers" that the computer can
understand. Sometimes this is implemented on the motherboards (USB), or
needs an additional card (frame grabber). It depends on the age of the
computer and its make and Operating system which of the different
options are supported.

3) Currently there are 3 ways of getting the signals into a PC (other
than serial RS-232 and parallel ports which are way to slow):

a) USB
b) 1394 or firewire
c) PCI boards

USB

USB is a serial interface that is now supported on most computers out of
the box. The bandwidth of a USB connection is a maximum of 12
MegaBIT/second, which translates of course into 1.5 Mega-BYTE per
second. This is too slow for video (about 4-5 MegaByte per second), but
enough for still images, unless the video is compressed. Compression is
OK for "consumer" video, but not acceptable for "scientific" video
unless it is lossless (JPEG and MPEG is usually not). Several devices on
the USB bus can compete for bandwidth.

1394

1394 (or firewire) is also a serial interface with a maximum bandwidth
of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast
enough for video. However, I believe that again more than one device can
be attached to a 1394 port which will again compete for bandwidth.
Firewire is not generally available on PCs (I believe the newer Macs
have it), so that for a firewire implementation on a PC normally a card
is necessary that fits into a PCI slot.

PCI

PCI boards, while a bit more cumbersome to install, have the highest
throughput. I think, they are now implementing a new PCI-X specification
that allows up to 1 GigaBYTE per second, i.e., about 20 times faster
than firewire. The reason is of course that PCI is a parallel standard
and not a serial like USB or firewire.

So, there are various ways to attach a camera:

Analog camera: There is no other way than to use a board to transform
the analog signals into digital signals and then send them to the
computer. This can be through a PCI or other card or other electronics
for example on the video card, but the translation is necessary.

Digital cameras: Digital cameras essentially put the digitizer into the
camera and then transmit digital signals. They can then be transferred
through USB (slow but available everywhere), firewire (faster, currently
on Macs (I believe) and PCs with additional card), or through boards for
the PCI bus (fastest, widely available, require board installation).

So, for most users (of PCs at least) there is currently no real
difference between using a firewire or other camera, as they either have
to install a PCI-} firewire card, or another PCI card for image
acquisition. That may change if the motherboard manufacturers start
building firewire circuitry into the motherboards and the operating
systems start supporting this option. For highest performance, however,
I believe that we will see PCI boards for some time to come. Firewire
may run into a performance problem in the future for image streams with
large images. A 1600x1200 image stream with 24 bit color and 30 frames
per second requires a bandwidth of about 170 MBytes/second uncompressed.

I hope I have not confused anybody with this.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================


} ----------
} From: White, Woody N[SMTP:WOODY.N.WHITE-at-MCDERMOTT.COM]
} Sent: Wednesday, January 26, 2000 4:13:00 PM
} To: "Peter Guthrie" ; Microscopy-at-sparc5.Microscopy.Com
} Subject: Re:USB or firewire cameras
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Peter,

If you intend to use a "video" camera, you may need a capture card
rather
than,
or in addition to, a USB or Firewire interface. Most video cams are
analog
(NTSC in the US, SECAM or PAL elsewhere in the world). Others can
certainly
have digital circuits internal (like the new digital camcorders or the
PCcams
used for video conferencing on the net). ...You first need to
determine
the
source format.

Warren's suggestion implements an inexpensive external NTSC* video frame
grabber
(Snappy). Which will "grab" an analog video frame and digitize it.
*May do
PAL
too??

Parallel port I/O is a bit slow (or is that a byte slow :) for pictures
containing lots of data, but is cheap and works since the inherent
resolution of
typical NTSC video is { 640x480. USB is very much faster and Firewire
faster
yet (and usually a lot more $$ for the interface card). For
applications
other
than full frame rate/resolution streaming video, USB is fine.

Woody White


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================




From daemon Thu Jan 27 17:58:03 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Thu, 27 Jan 2000 12:50:15 -0600
Subject: Re: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg,

I just downloaded from Netscape 4.6 and opened it with Acrobat 3.0

Damian



From daemon Thu Jan 27 17:58:03 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Thu, 27 Jan 2000 14:15:53 -0500
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael

The IMAC DV comes with a firewire port standard and a firewire cable.
Connection to a Sony DV camcorder is easy and it gives you complete control
from the computer. See http://www.apple.com/firewire/ for more information.

To get firewire into a PCI (mac or windows) machine Sony sells this card
http://www.sel.sony.com/SEL/consumer/ss5/office/digitalvideo/minidvcamcorderspro
ducts/dvbk-2000_specs.shtml for around $350 which gives similar controls
for live video and digital stills.

No interest in either company except as a satisfied customer.
Scott


}
} 1394 (or firewire) is also a serial interface with a maximum bandwidth
} of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast
} enough for video. However, I believe that again more than one device can
} be attached to a 1394 port which will again compete for bandwidth.
} Firewire is not generally available on PCs (I believe the newer Macs
} have it), so that for a firewire implementation on a PC normally a card
} is necessary that fits into a PCI slot.
}
..snip...
} So, for most users (of PCs at least) there is currently no real
} difference between using a firewire or other camera, as they either have
} to install a PCI-} firewire card, or another PCI card for image
} acquisition. That may change if the motherboard manufacturers start
} building firewire circuitry into the motherboards and the operating
} systems start supporting this option. For highest performance, however,
} I believe that we will see PCI boards for some time to come. Firewire
} may run into a performance problem in the future for image streams with
} large images. A 1600x1200 image stream with 24 bit color and 30 frames
} per second requires a bandwidth of about 170 MBytes/second uncompressed.
}
} Michael Bode, Ph.D.

-------------------note: new mailing address----------------------
Scott Wight e-mail: scott.wight-at-nist.gov
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | fax: 301-417-1321
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Thu Jan 27 17:58:06 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Thu, 27 Jan 2000 13:08:57 -0700
Subject: Re:No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree...

Chill out Fook

Sunny and 70 in Phoenix

-----Original Message-----
} From: Paul Webster [mailto:pwebster-at-hei.org]
Sent: Wednesday, January 26, 2000 6:52 PM
To: MSA listserver submission


} Please keep weather reports private.
}
} Ann Fook

Why? A little light heartedness never hurt anyone.
For the record, LA was sunny as usual today. Happy I don't live in CT
anymore.

Regards,

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Jan 27 17:57:45 2000



From: Pierre Ruterana :      ruterana-at-lermat8.ismra.fr
Date: Thu, 27 Jan 2000 16:47:52 -0800
Subject: Positions available in Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues

We would apreciate to inform interested young researcher of the
following available positions in our research project.
Thank you very much for getting this circulated.
Pierre

Pierre Ruterana
Laboratoire d'Etude et de Recherche sur les Materiaux (LERMAT)
Unite associee CNRS No 6004
Institut Superieur de la Matiere et du Rayonnement(ISMRA)
6, Bd Marechal Juin
14050 Caen Cedex
France
Tel: (33 2) 31 45 26 53
Fax:(33 2) 31 45 26 60 e-mail: ruterana-at-lermat8.ismra.fr




Research Training Network EC Contract N¡: HPRN-CT-1999-00040
Interface analysis at atomic level and Properties of Advanced Materials
(IPAM)

Eight positions are immediately available, eligible young ( { 35 years)
researchers must be citizens
of EC or associated countries (Norway, Island, Israel, Lichtenstein,
Bulgaria, the Czech Republic,
Estonia, Hungary, Lithuania, Poland, Romania, Slovakia, Slovenia and
Letonia), however any
foreigner who has spent five years in an EC country may apply. Women
candidates are particularly
encouraged to apply and equal opportunity between women and men will
govern our choice.
Following the mobility criteria, the young researchers will not apply
for a position in their native
country.

1. POSTDOCTORAL POSITION at Fritz Haber Institute, Max Planck Society,
Berlin,
Germany
A postdoctoral position at the Fritz-Haber-Institut in Berlin (Germany)
is available in the group
"Surface morphology and growth of semiconductors" under the supervision
of Dr. Joerg
Neugebauer. The research will be mainly focused on the theoretical
modeling of electronic
properties and atomic structure of interfaces and interfacial defects
employing first principles total
energy calculations. The basic materials for this project will be
gallium nitride based
semiconducting layers where extended defects are well known to occur in
large concentrations. The
research will be performed in close collaboration with experimental,
industrial, and theoretical
partners within the EC. Strong interaction with the other groups is
therefore expected. The
successful candidate should have a PhD in Physics, Chemistry or
Materials Science, and have a
strong interest on microscopic simulations. Preference will be given to
candidates with strong
background in any (or several) of these fields: electronic structure
calculations, molecular
modeling, density functional theory, empirical potentials, and analysis
of transmission electron
microscopy measurements.
Interested candidates should send immediately a CV, list of
publications, name and address
(including email) of three references, preferably by email or fax, to:
Dr Joerg Neugebauer
E-mail: neugebauer-at-fhi-berlin.mpg.de, Phone: ++49 30 8413 4826, Fax:
++49 30
8413 4701, www: http://www.fhi-berlin.mpg.de/th/JG

2. POSTDOCTORAL POSITION at Universitat Politcnica de Catalunya,
Barcelona, Spain
A postdoctoral position is available at the department of Applied
Mathematics in the UPC. We are
seeking a computational materials scientist interested in modeling the
atomic structure of interfaces
and defects in crystals, mainly gallium nitride based materials. A PhD
in Physics, Materials Science
or related discipline and having experience with atomic simulations is
required. It is highly
desirable an ability to develop empirical interatomic potentials. It is
intended that he/she visits the
other laboratories working in the project to learn how to interpret the
experimental observations and
how to use the theoretical concepts.
Interested candidates should send immediately a CV, list of
publications, name and address
(including email) of three references, preferably by email or fax, to:
Prof. Anna Serra
E-Mail: a.serra-at-upc.es, Phone: ++34 93 401 68 86, Fax: ++ 34 93 401 18
25

3. A FULL TIME PhD STUDENT at CRHEA, Valbonne, France
These last years, CRHEA-CNRS has implemented an expertise in the growth
of
heteroepitaxial GaN layers on different substrates: sapphire, SiC and Si
by different techniques,
MBE, MOVPE and HVPE. The group has developed a proprietary Epitaxial
Lateral Overgrowth
(ELO) technology which allows to decrease by orders of magnitude the
density of dislocations in
GaN heteroepitaxial layers on sapphire, SiC or Si. Therefore, a great
interest in the procurement of
high quality GaN substrates currently exists. The successful candidate
will strongly support our
present effort to produce self-supported GaN of ELO quality by combining
ELO-MOVPE and
HVPE. Parallel to the growth, he will contribute to the development of
in depth analysis of basis
mechanisms linked to the generation and propagation of threading
dislocations(TDs). More
precisely, it is planned to determine the core structure of defects in
ELO GaN, their electronic
structures (by EELS), the mechanism of bending of these TDs, to
implement new ways of further
decreasing the density of dislocations. He will be able to use two
MOVPE, one HVPE reactor and
all basic characterisation tools (double X-ray diffraction,
magnetotransport, low temperature
photoluminescence, HRTEM,.).
Candidates should send immediately a CV, name and address (including
email) of two references,
preferably by email or fax, to:
Dr Pierre Gibart, email: Pierre.Gibart-at-crhea.cnrs.fr, Tel: ++33 4 93 95
42 27, Fax: ++33 4
93 95 83 61
CHREA-CNRS is located at the French Riviera near Nice ( see:
http://www.crhea.cnrs.fr/)

4. POSTDOCTORAL POSITION at ISMRA Caen, France
Candidates should preferably have a Phd with experience in electron
microscopy and/or atomic
structure modeling. The project will involve experimental high
resolution electron microscopy and
image analysis. In parallel, atomic structure modeling of defects and
interfaces will use empirical
and tight binding methods. A connection will be established with ab
initio techniques developed in
partner groups and the successful candidate will undertake quantitative
comparison of experimental
and simulated images. The overall aim is the understanding of the role
of defects and interfaces on
the optoelectronic properties in the Ga based nitride semiconductors.
Candidates should send immediately a CV, list of publications, name and
address (including email)
of three references, preferably by email or fax, to:
Dr Gerard Nouet on ++33 2 31 45 26 47,
email:gerard.nouet-at-labolermat.ismra.fr or Dr
Pierre Ruterana on ++33 2 31 45 26 53 email: ruterana-at-lermat8.ismra.fr

5. POSTDOCTORAL POSITION at University of Liverpool, Great Britain
A three year full-time appointment funded by the European Commission is
available to study defect
mechanisms in gallium nitride based electronic device structures within
the III-V semiconductor
materials group. Candidates should preferably have postgraduate
experience in the growth or
processing of semiconductor device materials. The project will involve
the chemical beam epitaxy
of GaN based materials and the fabrication of model device structures.
The influence of processing
parameters on defect propagation will be investigated using analytical
methods such as electron
microscopy, Raman spectroscopy and surface analytical techniques. This
appointment is part of a
Research Training Network and eligible candidates must be citizens of EC
member countries other
than the United Kingdom.
Candidates should send immediately a CV, list of publications, name and
address (including email)
of three references, preferably by email or fax, to:
Dr Paul Chalker: ++44 151 794 4313, email pchalker-at-liv.ac.uk or Prof
Robert Pond: ++44
151 794 43 13 / 46 60, email R.C.Pond-at-liverpool.ac.uk

6. POSTDOCTORAL POSITION at UniversitŠt Erlangen-NŸrnberg, Germany
Focus of the work in Erlangen university will be on direct correlation
of structural, optical and
electrical properties of extended defects in (i) group-III nitrides (ii)
group III-nitride based
heterostructures. Experimental work is based on (scanning) transmission
electron microscopy
((S)TEM) at all levels of resolution. These comprise high resolution
imaging with atomic
resolution, optical characterisation by cathodoluminescence in the STEM
and analysis of electrical
properties (electrical activity of extended defects, diffusion length of
minority carriers) by the
electron beam induced current (EBIC) both in the SEM and the STEM.
Theoretical analyses are
based on TEM contrast simulation for defect analysis, analysis of the
tetragonal distortion from
high resolution TEM images and finite element simulations of the
strained state of heterostructures.
Candidates should send immediately a CV, list of publications, name and
address (including email)
of three references, preferably by email or fax, to:
Prof Horst Strunk, Tel: ++49 9131 85 2 8601, Fax: ++49 9131 85 2 8602,
email:
strunk-at-cmp03ww7.ww.uni-erlangen.de, Dr Martin Albrecht, Tel.: ++49
9131 85 2 8613,
Fax: ++49 9131 85 2 8602, e-mail: albrecht-at-cmp04ww7.ww.uni-erlangen.de,


7. POSTDOCTORAL POSITION at the Aristotle University of Thessaloniki,
Greece
A research opportunity is available for postdoctoral candidates with a
background in Electron
Microscopy, Crystal Growth, Materials Science. The primary
responsibility of the candidate will
be the study of the structure and properties of the heterophase
interfaces between thin films on
gallium nitride based materials. The project will offer the necessary
training for the specific skills
to meet the requirements of the job.
Candidates should send immediately a CV, list of publications, and name
and address (including
email) of three references, preferably by email or fax, to:
Prof Philomela Komninou, Tel: ++30 31 99 81 95 / Fax: ++30 31 99 80 61
e-mail: komnhnoy-at-auth.gr

8. A FULL TIME PhD STUDENT at The University of Cambridge, Great Britain
A three year research studentship position leading to a PhD degree is
available to study the
microscopy and analysis of defects in gallium nitride layers and device
structures. This exciting
project will use a wide range of state-of-the-art electron microscopy
and analysis techniques to
study the atomic structure of defects (using high resolution electron
microscopy), their chemical
composition and electronic properties (using x-ray spectroscopy and
electron energy loss
spectroscopy), including which defects give rise to states in the band
gap. This project is part of a
European Research Training Network aimed at optimising devices in
GaN-based materials. The
research student will form strong links with the other European partners
in this project. Eligible
candidates should have a top quality degree in physics, chemistry,
materials science or electrical
engineering.
Candidates should send immediately a CV, name and address (including
email) of two references,
preferably by email or fax, to:
Prof Colin Humphreys on +44 1223 334457, email
{colin.humphreys-at-msm.cam.ac.uk} , or
Dr Dave Tricker on +44 1223 334469, email {dmt1000-at-cus.cam.ac.uk}


The Candidates will gain time by sending a copy of their CV also to
Prof. Philomela
Komninou, leader of the Training Programme. Tel: ++30 31 99 81 95 /
Fax: ++30 31 99 80 61
e-mail: komnhnoy-at-auth.gr; Please indicate the position of interest.


Caen, January 27 2000
Dr. Pierre Ruterana
Coordinator



From daemon Fri Jan 28 07:53:17 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 28 Jan 2000 15:13:15 +1000
Subject: RE: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems nobody replied to Michaels question. Perhaps because there is no
single answer. LR White and LR Gold I would expect to have a very similar
shelf-life - under similar conditions.

The trouble is to know the starting point. LR White slowly "goes off" from
when the catalyst is added. At room temperature or higher this happens at a
much faster rate than when it's kept refrigerated. Catalyzed LR White could be
kept at the room temperatures for some months. Because the shipping time (even
by air) to our home-market (Australia) from the UK is too long and often at
high temperatures, much and an indeterminable part of the shelf-life would be
expired prior to sale.

Consequently we only procure uncatalysed LR White and the end-user must add and
thoroughly mix the catalyst prior to first use. Our users expect a full year of
refrigerated shelf-life. Shelf-life is really a "when, how long and at what
temperature" question.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, January 25, 2000 4:52 PM, Michael Reiner
[SMTP:michael.reiner-at-Smail.Uni-Koeln.de] wrote:
}
}
} Dear members of the list,
}
} first, I would like to wish you all the best for the new year.
} Now my question:
} Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?
}
} Is it that delicate as LR-White (Meanwhile I don?t use LR-W older than
} half a year). My bottle which was not opened many times, could be
} roundabout three years old.
}
} Thanks a lot,
} Michael
}
} Michael Reiner
} Department of Anatomy I
} University of Cologne
} Germany
} michael.reiner-at-smail.uni-koeln.de



From daemon Fri Jan 28 07:53:25 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Fri, 28 Jan 2000 09:42:49 CET
Subject: Re:No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*Date sent: 26 Jan 00 17:52:26 -0800
*From: Paul Webster {pwebster-at-hei.org}
*Subject: Re:No weather report please
*To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
*Send reply to: Paul Webster {pwebster-at-hei.org}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jan 28 07:53:33 2000



From: Timothy Dimitri :      tdimitri-at-us.ibm.com
Date: Fri, 28 Jan 2000 07:56:57 -0500
Subject: SEM - Manufactures of Microcleave tools for silicon wafers???

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I am looking for the names of any manufactures (other than SELA) that
have a product that can cleave sub micron features on silicon wafers...

Thank you

Timothy Dimitri
ASTC Failure Analysis Laboratory
IBM Microelectronics Division



From daemon Fri Jan 28 08:08:17 2000



From: McGill, Ricky L :      rlmcgill-at-eastman.com (by way of Nestor J.
Date: Fri, 28 Jan 2000 07:55:51 -0600
Subject: Spring 2000 AReMS Meeting

Contents Retrieved from Microscopy Listserver Archives
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Dear list members,
The intent of this note is to formally announce a call for papers
for the upcoming Spring 2000 AReMS (Appalachian Regional Microscopy
Society) meeting to be held in Raleigh, NC.The meeting dates are March 30
and 31.
The theme for this meeting is "Recent Advances in Microscopy for
2000",specifically we would like to focus on recent (but not limited to)
advances in microscopical instrumentation and applications therefrom.


Please forward any abstracts, papers or related items to me at
mailto:\\rlmcgill-at-eastman.com or you may contact me at the number
below.

The most recent meeting info is -at-
http://www.wise.virginia.edu/cvc/arems/raleigh.html

Thanks in advance for your interest!


Rick McGill
Microscopy Research
Eastman Chemical Company
- - - Phone: (423) 229-5473
- - - Fax: (423) 229-4558
- - - e-mail: rlmcgill-at-eastman.com




From daemon Sat Jan 29 08:54:12 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 28 Jan 2000 14:13:01 +0000
Subject: Re: RE: No weather report please

Contents Retrieved from Microscopy Listserver Archives
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You should logon to Histonet - they're much friendlier!

Had a beautiful drive from old Plymouth to Reading, Berkshire, with
my retired boss yesterday to collect x-ray microanalysis equipment.
Clear blue sky, -2 to +5 degrees. Saw the most gorgeous scenes of
trees covered by thick hoar frost - photographers' dream. Met two
nice ladies - Hello, Jill and Hilary! Thanks for the help!

Keith Ryan
Marine Biological Association of the UK
PS - Don't read this if you don't like the weather !
PPS - See, Paul, I did get there!
PPS - Hello, Daniele - time to write!



From daemon Sat Jan 29 08:54:09 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 28 Jan 2000 14:23:12 +0000
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You ought to logon to "Histonet" - they're much friendlier!

I agree about the weather being important to EM. 20-30 years ago we
had some seaweed hanging in the microtome room (about 100 m from the
sea). If it was damp we didn't even try cutting some resins!

Had a car trip yesterday from old Plymouth to Reading, Berkshire,
collect EM equipment., -2 to +5 degrees, clear blue sky, gorgeous
scenes of thick hoar frost on the winter trees - a photographers'
dream. Bonus - met two nice girls - Hello, Jill and Hilary, thanks for
the help!

Keith Ryan
Marine Biological Association of the UK
PS - Don't read this if you don't like weather!
PPS - Paul, I made it!
PPPS - Hello, Daniele, its time you wrote!



From daemon Sat Jan 29 08:54:18 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 28 Jan 2000 11:14:34 -0600 (CST)
Subject: weather & Formvar films

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Dear Microlisters,

Neither rain nor sleet, snow nor heat, humidity, hell nor highwater can keep me
from getting Formvar films off glass slides. Only time can...see below.

My secret? Just rinse glass slides - both sides & all around edges, except for
the end you are holding onto - with 95% ethanol and air dry. Then use right away
- dunk into Formvar solution (.25 to .5% w/v in ethylene dichloride), drain and
air dry. Score around edges to break film and float off onto clean water
surface. I like to score the edge by using the corner of a razor blade to score
on the top surface of the slide, near the edge, in addition to scoring the
actual corner edge of the slide with the blade held perpendicular to the edge -
know what I mean? Also, score across the slide near the "top" edge of the
Formvar film, near the end you are holding on to, for clean release of the end
of the film.

Second point, Formvar film solutions older that 3 months tend to stick to glass.
I've been tracking that for years. Put mix date on bottle of fresh solution,
after 3 months expect poor release effects to appear.

Gib

P.S. I tend to agree with Fook that this forum should not be used to discuss the
weather ONLY, but if someome wants to put a current local weather "tag" at the
end, AFTER the Microscopy stuff, with their signature, thats OK with me.
Everyone is entitled to their (short) bit of poetry, sage sayings, or weather
commentary there.

For example: "The weather here is unremarkable at this time."

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Sat Jan 29 08:54:19 2000



From: Ray D. Twesten :      twesten-at-uiuc.edu
Date: Fri, 28 Jan 2000 11:52:18 -0600
Subject: TEM: Help with Microtoming Nanophase Metals

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Dear All,
I have a user who is trying to make TEM samples from nanophase metals.
They come out of the process as a fine (10 to 100um) powder. The study is
to compare the microstructure at different processing temperatures (77K to
400K). The current sample preparation technique is to embed the powder in
epoxy, slice and polish, and finish with l-N2 ion milling (BTW, direct
dispersion of the powder does not work as the edges are too thick). There
are several problems with this technique, the worst being that we have
found these materials age rapidly even at room temperature. The epoxy cure
and ion milling thermal budgets may be a problem.
I am considering ultramicrotomy for these samples, but I have zero
experience in this field. McMahon and Malis (1995 Micro Res & Tech v31 267)
worked on a similar system and outline the use of thermally cured LR-White
as the embedding material, so I think it is an appropriate option, but I am
worried about the thermal budget. My question is:
Does any one have experience with low-temperature, UV cured resins for
materials of these type? If so I would greatly appreciate any advice.

Thanks in advance,
Ray


*************************
Ray D. Twesten, PhD
Center for Microanalysis of Materials
University of Illinois
(217) 244-6177 fax:(217) 244-2278




From daemon Sat Jan 29 08:54:22 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 28 Jan 2000 15:21:14 -0600
Subject: Re: ICC label on cell walls

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Please see request below. If you have any suggestions, either send to the list and I will forward them to the investigator, or send them directly to Dale.
Thanks for the input.
Debby Sherman

--------------------------------------


Greetings,
I can make two suggestions.

1) Ignore the staining in the cell wall. Since you know that
your protein of interest is not there and you know also that
secondary cell wall must be outside of the cell, this should not
interfere with staning inside the cell.

2) Try to pre-absorb with "wood". The idea here would be to
incubate your serum with some sort of wood pulp, and then spin out
the wood pulp presumably taking with it all the ab's that bind wood,
but leaving behind the ones of interst. I am not sure how best to
make a suitable pulp of wood. Maybe take a pencil sharpener and grind
a dowell, and then grind the shavings further in a mortar and pestle
or maybe use a homogenizer of some sort. I am guessing wildly here.
Certainly the wood bits should be easy to spin after absorption.

Hope this helps,
Tobias.


}
} Date: Thursday, January 27, 2000
} } From: Dale Karlson {dtk-at-omni.cc.purdue.edu}
}
}
}
} QUESTION: How to minimize artifact labelling with secondary cell walls
}
}
} To whom it may concern:
}
} I am attempting to localize protein in a woody plant and consistently
} observe an interaction with secondary cell walls. This is an artifact, we
} know that the protein does not exist in the cell wall. We are using a
} polyclonal antibody that was raised against a protein that was excised
} from an SDS gel, suspended in Freund's complete adjuvant and used for the
} immunization (in chicken). Chicken antibodies were purified by an
} ammonium sulfate precipiation method described by Song et al. (Song, C.S.,
} J.H. Yu, D.H. Bai, P.Y. Hester, and K.H. Kim. 1985. Antibodies to the
} 5-subunit of insulin receptor from eggs of immunized hens. Journal of
} Immunology 135: 3354-3359). I have tried Western blot affinity
} purification of the antigen and antibody and it has not solved this
} problem. We do not have access to a "purified" form of the protein, so
} affinity purification with a purified protein is not an option.
}
} It is obvious that the chicken had an "allergy" that was not visible
} during our screening process (with western blots) to select the host
} animal. The chicken obviously has specific antibodies to some secondary
} wall component, and I would like to know what it might be and how I could
} remove this artifact.
}
} Any input would be greatly appreciated.
}
}
} -------------------------------------------
}
} Debbie,
}
} let me know if this is suitable..or if it is way too long etc.
}
} Thanks,
}
} -Dale
}
}
}
}
}
} _______________________________________________________________________
}
} Dale Karlson .***. .***. .***.
} 1165 Horticulture Building * | | | * | | | * * | | | *
} Purdue University * | | | * * | | | * * | | | *
} W.Lafayette. IN 47907-1165 * | | | * * | | | * | | | *
} '***' '***' '***'
}
} Home Phone: (765) 742-8379
} Lab Phone: (765) 494-1345
} _______________________________________________________________________

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Sat Jan 29 08:54:27 2000



From: Jim Goodman :      jgoodman-at-utsi.edu
Date: Fri, 28 Jan 2000 14:07:42 -0800
Subject: TEM

Contents Retrieved from Microscopy Listserver Archives
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To All,

The decision has been made to get rid of the following piece of equipment. We obtained the unit approximately five years ago when an outside Contractor brought the system very near operational state. Numerous distractions and circumstances have prevented the TEM from becoming the valuable research investigative tool we had planned.

HITACHI H-600 TEM

1) Model H-600-1 Analytical Electron Microscope
2) Model H-6015 EDX Interface Kit
3) Model H-6012 Micro-Diffraction Unit
4) Spot Scan
5) Model H-6006 Auto Data Display Unit
6) Reduced Area Scan Unit
7) Polaroid Camera w/ Adaptor
8) Model H-6007 High Resolution CRT
9) Model H-5001-C Cobling Holder
10) 2 ea. Overhauled Mechanical Vacuum Pumps & 1 ea. Diffusion Pump

Anyone interested in requesting a Bid Form should contact me at following address:

Jim Goodman
University of Tennessee Space Institute (UTSI)
411 B. H. Goethert Pkwy.
Tullahoma, TN 37388
TEL: (931) 393-7494
FAX: (931) 393-7543
e-mail: jgoodman-at-utsi.edu



From daemon Sat Jan 29 08:54:21 2000



From: Jo Dee :      jofish-at-burnham-inst.org
Date: Fri, 28 Jan 2000 22:32:52 -0800
Subject: Re: No weather report please

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Yes, I also agree. Today in San Diego it is about 68 degrees with 76% humidity.
Great for cryoultramicrotomy and regular cutting!
Take care all,
Jo Dee

Witold Zielinski wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} *Date sent: 26 Jan 00 17:52:26 -0800
} *From: Paul Webster {pwebster-at-hei.org}
} *Subject: Re:No weather report please
} *To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} *Send reply to: Paul Webster {pwebster-at-hei.org}
}
} *------------------------------------------------------------------------
} *The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} *To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} *On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} *-----------------------------------------------------------------------.
} *
} *
} *} Please keep weather reports private.
} *}
} *} Ann Fook
} *
} *Why? A little light heartedness never hurt anyone.
} *For the record, LA was sunny as usual today. Happy I don't live in CT anymore.
} *
} *Regards,
} *
} *Paul Webster, Ph.D.
} *Associate Scientist & Director
} *Ahmanson Advanced Electron Microscopy & Imaging Center
} *House Ear Institute
} *2100 West Third St.
} *Los Angeles, CA 90057
} *
} *Phone: (213) 273-8026
} *Fax: (213) 413-6739
} *e-mail: pwebster-at-hei.org
} *http://www.hei.org/htm/aemi.htm
} *
} *
} I agree with you Paul.
} In Warsaw, Poland yesterday was snow on the ground today is
} rain and no chance for sunshine.
} Stay cool,
} Witold

--
Jo Dee Fish
Electron Microscopy Assistant
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
858-646-3100 ext.3620




From daemon Sat Jan 29 08:54:52 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sat, 29 Jan 2000 11:13:44 +0000
Subject: Double weather report - sorry

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The first one took a long time to appear - so I thought it hadn't made
it. Must've encountered head winds!

Keith



From daemon Sat Jan 29 08:54:53 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sat, 29 Jan 2000 11:17:31 +0000
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
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Sorry for wasting time with two messages.

The first weather report took so long to appear that I thought it
hadn't made it. Must've encountered headwinds!

Enough - Keith Ryan
Marine Biological Association of the UK
- where its now wet and windy
PS - Hi Daniele



From daemon Sat Jan 29 13:04:49 2000



From: Diane G. Miller :      millerd-at-coho.net
Date: Sat, 29 Jan 2000 09:12:49 -0600
Subject: Cutting live cells on Vibratome

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Is anyone cutting live cells on a vibratome? These are from cell cultures.
If anyone has had experience with this, I would appreciate the information.

Thanks
Diane
millerd-at-coho.net




From daemon Sat Jan 29 13:04:48 2000



From: Cochran :      fisher-at-meganet.net
Date: Sat, 29 Jan 2000 10:30:45 -0500
Subject: service providers needed

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

This request is posted on behalf of an associate who does not subscribe
to this list. He has assumed responsibility for the following equipment
and is looking for service providers. Equipment is located in south
central Massachusetts.

Jeol 840A sem
Kevex delta 5 EDS & XRF with Syquest drive upgrade

He should be contacted off line by e-mail at LapradeB-at-burle-eo.com

Thanx,
Ray



From daemon Sat Jan 29 13:04:52 2000



From: IMZartTchr-at-aol.com
Date: Sat, 29 Jan 2000 13:09:29 EST
Subject: microbiological staining technique

Contents Retrieved from Microscopy Listserver Archives
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what microbiological staining technique could be used to allow you
to differeniate Bordetella bronchiseptica from Vibrio cholerae with certainty?



From daemon Sun Jan 30 08:44:58 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Sat, 29 Jan 2000 18:40:43 -0600
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
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Michael,

FYI, I just tested an Optronics digital camera that had a on board firewire
connection to a Gateway 366MHz notebook computer. One can also get PCMCIA
card to connect to most notebook computers. Nice camera but should be for
$13k, without notebook computer!

Damian


} 1394 (or firewire) is also a serial interface with a maximum bandwidth
} of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast
} enough for video. However, I believe that again more than one device can
} be attached to a 1394 port which will again compete for bandwidth.
} Firewire is not generally available on PCs (I believe the newer Macs
} have it), so that for a firewire implementation on a PC normally a card
} is necessary that fits into a PCI slot.



From daemon Sun Jan 30 08:45:03 2000



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Sat, 29 Jan 2000 21:54:07 -0600
Subject: Looking for Ion Beam sputter coater or similar

Contents Retrieved from Microscopy Listserver Archives
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I'm trying to find a used ion beam sputter coater or something similar...
The system I am used to is the old VCR group ion beam sputter coater. Any
leads that you might have would be greatly appreciated...

Thanks,

Raj

*********************************************
Raj Lartius, Ph.D.
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Web: www.novascan.com
**********************************************



From daemon Sun Jan 30 12:05:00 2000



From: bobrob-at-uswest.net
Date: Sun, 30 Jan 2000 10:18:55 -0700
Subject: TEM/EDS Detector Wanted

Contents Retrieved from Microscopy Listserver Archives
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We are interested in acquiring an EDS detector for our JEOL
JEM-1200EXII.
Will trade a Kevex Be-windowed 30mm2 that was formerly attached to an
EM400 for a Kevex/TN/Noran 10mm2/30mm2 Be or UTW. Need detector
only (no MCA, P. Processor, etc). Will purchase or trade.

If you have a suitable detector and are in a position to trade/sell
immediately,
please reply off line to sender.

Bob Roberts
EM Lab Services, Inc
2409 S. Rural Rd
Tempe, Arizona 85282
(480) 967-3946



From daemon Mon Jan 31 07:34:26 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Sun, 30 Jan 2000 11:53:22 -0500
Subject: Double immunofluorescence artifacts

Contents Retrieved from Microscopy Listserver Archives
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I have been reading about FRET and have a question about dual label
imaging with probes like FITC/RHo or Cy3/Cy5. We have to worry about
excitation of the long wavelength probe at the shorter probe
wavelength, and FRET as two ways in which we can be mislead about the
co-localization of two probes. I am wondering to what extent one
also has to worry about non-FRET energy transfer. It seems that
there is some possibility that, for example, Cy5 could become excited
by absorbing photons from Cy3 emission. My presumption is that the
density of photons is low, and this would limit the effect, but it
seems that as proximity gets closer, the chances of this radiative
exchange (rather than resonance exchange) would become greater. Are
there any experimental guidelines as to when to worry about this?
Thanks- Dave
--

************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Mon Jan 31 07:34:26 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Sun, 30 Jan 2000 20:08:22 -0800 (PST)
Subject: Re: Bio Cryo

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On Mon, 24 Jan 2000 13:33:28 -0500, Greg Erdos wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Periodically questions appear on this list concerning the use of
cryostats
} for cutting histological sections. I usually reply to the sender off
line
} and offer a copy of a handout that I got from a workshop at a Histochem
} Meeting some years ago. Even though it is old, cryostat sectioning has
not
} changed a lot. I think there is some valuable info there, especially for

} beginners. I thought it might be helpful to make this available on the
net
} for whomsoever might want to take a look.
} It can be found at :
} http://www.biotech.ufl.edu/sems/
}
} Look for the snowflake
}
} It was written by Bruce Quinn, then of MIT. I hope he has no
objections
} to my posting it.
}
} Greg Erdos
} Gregory W. Erdos, Ph.D. Ph.
352-392-1295
} Assistant Director, Biotechnology Program
} PO Box 110580
Fax:
} 352-846-0251
} University of Florida
} Gainesville, FL 32611
}
}
}
Finally got onto things here at home, and using Netscape 4.5 and Acrobat
3.0, the document opens up the way it should. Thanks for the info and link,
Greg.

Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Mon Jan 31 07:34:37 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Mon, 31 Jan 2000 08:57:00 -0500
Subject: 2nd annual course in fluorescence microscopy

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Dear Timothy

A method I've used for controlling the position of the cleave in silicon
wafers is to use focused ion beams (FIBs) to mill micro-cleaving grooves
into the silicon. I found that the grooves can determine the position of a
cleave to within 200 nm.

If you want any more details I can forward you a pre-print on the technique.

Richard

--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273729, Fax: +44 (0)1865 273794
email: richard.langford-at-materials.oxford.ac.uk

----- Original Message -----
} From: Timothy Dimitri {tdimitri-at-us.ibm.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, January 28, 2000 12:56 PM


Folks:
I thought I should let you all know about the Second annual course in
Quantitative Fluorescence Microscopy to be taught between june 19 and 24th
2000 at the Mount Desert Island Marine Biology Laboratories in Arcadia
National Park in Maine. This team taught course led by Dave Piston
(Vanderbilt), Mike Nathanson (Yale), Guillermo Romero (Pittsburgh) and
myself focusses specifically on the development and application of modern
fluorescence microscopic methods. This intensive course covers all aspects
of the technology from microscope and dye design, cameras, confocal
microscopy, live cell microscopy, multiphoton microscopy and GFP.
Considerable attention is also given to quantitative analysis in 2 and 3
dimensions and time. The specific focus of the course allows an in depth
treatment of these methods. The goal of the course it to teach students how
to best implement these methods within their labs, using either their own
cells and tissues or using material supplied by the course. An extensive
array instrumentation, provided by all the major microscope and associated
software, hardware and camera manufacturers will be available for students
to use.
Last year it was a very successful event and we were encouraged to give the
course again. A full description of the course lectures together with
lecture outlines, registration etc. is available at
http://sbic6.sbic.pitt.edu/microscopy. I would encourage you to spread the
word, or sign up if you are interested. The total number of students is
limited to 20, enrollment is decided by the course faculty.
If you have any further questions please feel free to contact me
Thanks
Simon


-----------------------------------------
Simon C. Watkins Ph.D. MRCPath
Associate Professor
Director: Center for Biologic Imaging
University of Pittsburgh
Pittsburgh PA 15261
tel:412-648-3051
fax:412-648-8330
URL: http://sbic6.sbic.pitt.edu
-----------------------------------------
Simon C. Watkins Ph.D. MRCPath
Associate Professor
Director: Center for Biologic Imaging
University of Pittsburgh
Pittsburgh PA 15261
tel:412-648-3051
fax:412-648-8330
URL: http://sbic6.sbic.pitt.edu



From daemon Mon Jan 31 18:30:41 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 31 Jan 2000 11:02:16 -0400
Subject: Re: microbiological staining technique

Contents Retrieved from Microscopy Listserver Archives
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At 1:09 PM -0500 1/29/0, "IMZartTchr-at-aol.com"-at-sparc5.Microscopy.Com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

***********
If you don't get an answer from MSA people, try the listserver for the
histologists out there:
"HistoNet Server" {HistoNet-at-Pathology.swmed.edu}

Lee

Lee Cohen-Gould
EM & Confocal Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207




From daemon Mon Jan 31 18:30:48 2000



From: ctschristopher :      ctschristopher-at-samiot.uct.ac.za
Date: Mon, 31 Jan 2000 17:36:52 +0300
Subject: TEM Video imaging

Contents Retrieved from Microscopy Listserver Archives
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Hi folks

The Cape Town weather is great today, beautiful clear blue skies and a nice
warm 25 C.

We have a Philips TEM 420 which has an EDAX system attached (which is
non-functional at the moment). We are looking for video / digital image
grabbing system that we can use to grab images for prints and possibly Image
analysis. Is this possible on the 420 ? Can anyone suggest a system?

Thanks

Phil


Phillip Christopher
Cardiovascular Research,UCT
Anzio Road, Observatory, 7925
27-21-4066613/6476(tel)
27-21-4485925(fax)
ctschristopher-at-samiot.uct.ac.za



From daemon Mon Jan 31 18:30:49 2000



From: Joyce Craig :      j-craig-at-CSU.EDU
Date: Mon, 31 Jan 2000 10:06:12 -0600
Subject: TEMSEM Halophytes

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A graduate student here (Rita Ware) had some success fixing halophilic
bacteria for TEM several years ago. She used the growth medium as a
buffer. She made a poster presentation at an MSA meeting.



From daemon Mon Jan 31 18:30:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 31 Jan 2000 11:54:48 -0500 (EST)
Subject: Re: Reichert Ultracut E with cryo FC 4D

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Call TekNet: 800 835-6386

On Mon, 24 Jan 2000, Marti, Jordi wrote:

} Date: Mon, 24 Jan 2000 06:46:26 -0700
} From: Marti, Jordi {jordi.marti-at-honeywell.com}
} To: 'Microscopy' {Microscopy-at-sparc5.Microscopy.Com}
} Subject: Reichert Ultracut E with cryo FC 4D
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The power supply in our cryo microtome is having problems which might be
} related to the transformer. I was told by the service engineer that the
} transformer is no longer supported by Reichert. Does any one know where I
} can get a replacement ?
}
} Thanks
}
} Jordi Marti
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Mon Jan 31 18:30:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 31 Jan 2000 12:06:45 -0500
Subject: SEM - Manufactures of Microcleave tools for silicon wafers???

Contents Retrieved from Microscopy Listserver Archives
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Dear Timothy:

South Bay Technology, Inc. manufactures an SEM Cleaving System which
provides a means to precisely and quickly cleave a wafer while in the
inspection mode. A wafer is mounted to a vacuum chuck which is positioned
under an optical microscope. The exact area of interest is located
visually and the sample is cleaved at that point. SEM compatible versions
of the cleaving system are under development which will allow the user to
image and cleave while mounted in the SEM. The Cleaving System is quick,
easy to operate and precise. It allows anyone to quickly and repeatably
prepare SEM cross sections.

If you have an interest, please let me know and we can discuss your
requirements in detail.

Best regards-

David
Writing at 11:48:36 AM on 01/28/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.


Message text written by Timothy Dimitri
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am looking for the names of any manufactures (other than SELA) that
have a product that can cleave sub micron features on silicon wafers...

Thank you

Timothy Dimitri
ASTC Failure Analysis Laboratory
IBM Microelectronics Division


{



From daemon Mon Jan 31 18:30:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 31 Jan 2000 12:06:43 -0500
Subject: TEM - Manufactures of Microcleave tools for silicon wafers???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Timothy:

I forget to mention in my original posting that South Bay Technology also
produces the MicroCleave kit which is designed for TEM cross sectioning.
Again, if you have an interest, please let me know and I'll get you
additional information.

Best regards-

David
Writing at 11:53:19 AM on 01/28/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by Timothy Dimitri
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am looking for the names of any manufactures (other than SELA) that
have a product that can cleave sub micron features on silicon wafers...

Thank you

Timothy Dimitri
ASTC Failure Analysis Laboratory
IBM Microelectronics Division

{



From daemon Mon Jan 31 18:30:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 31 Jan 2000 12:06:40 -0500
Subject: Looking for Ion Beam sputter coater or similar

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Lartius:

While I don't have any leads on a used IBS system, I wanted to let you know
that we at South Bay Technology, Inc. are continuing the manufacture of the
IBS system formerly produced by VCR Group. Actually, we have updated the
system and are now offering the IBS/E. The IBS/E now adds the capability
of etching samples as well as coating and will accommodate samples up to 2"
in diameter.

If you would like additional information, please feel free to contact me.

Best regards-

David
Writing at 9:48:22 AM on 01/31/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "Dr. Raj Lartius"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm trying to find a used ion beam sputter coater or something similar...
The system I am used to is the old VCR group ion beam sputter coater. Any
leads that you might have would be greatly appreciated...

Thanks,

Raj

*********************************************
Raj Lartius, Ph.D.
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Web: www.novascan.com
**********************************************

{



From daemon Mon Jan 31 18:30:54 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 31 Jan 2000 11:06:50 -0700 (MST)
Subject: Sandbox Squabble-Weather

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Are we like kids sitting in a sandbox and squabbling? Yes weather, no
weather! He said it first! She started it! I don't want weather on the
listserver. I want to read about weather in the boondocks. Who cares
about you, anyway? Sort of funny when you think of it that way, isn't it?

Bye guys,
Hildy Crowley



From daemon Mon Jan 31 18:30:57 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 31 Jan 2000 08:49:42 -1000 (HST)
Subject: SEM: JEOL JSM840-AM1000

Contents Retrieved from Microscopy Listserver Archives
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Hi, microscopists-

A friend has the chance to buy a used JEOL JSM840 - AM1000 with EDX and
all the bells and whistles. He has asked if this is a good instrument,
and worth the price (whatever that is - he won't tell me). Since I
haven't seen the instrument and I don't know JEOLs at all, I told him I
would ask the experts.

If anyone can tell me a little about the instrument and what it might be
worth (the second part of the question being more difficult), I would
appreciate it.


In Honolulu it is gloriously clear and sunny, with temperatures near 80F
during the day and about 60F at night, which is unusually cold but really
nice.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Jan 31 18:31:10 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Mon, 31 Jan 2000 13:32:03 -0800
Subject: Teaching/Outreach call for papers

Contents Retrieved from Microscopy Listserver Archives
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You are invited to participate in a symposium of remote access microscopy,
and /or teaching microscopy to take place at the Microscopy &
Microanalysis Annual Meeting August 13-17, 2000 in Philadelphia, Pa.

Platform and poster contributions are welcome. Please contact me
directly for more information about the symposium.

Deadline for receipt of a 2page abstract is Feb 15, 2000. For
registrationand abstract forms, see
http://www.microscopy.com/MSAMeetings/MMMeeting.html

ADVANCES IN INSTRUMENTATION AND TECHNIQUES SYMPOSIUM 19: TEACHING
MICROSCOPY IN THE NEW MILLENNIUM

Organizer: Steve Barlow

The use of computers to control microscope operations, the ability to
control microscopes remotely over the Internet, and the creation of
microscope computer simulations allow researchers and students to access
microscopes in new ways. These developments mean changes in the way
microscopy can be taught to students and researchers. This symposium will
examine different ways to teach microscopy and microscope theory and
operation to reseachers and students of all levels, in the context of new
laboratory configurations, computer simulations, remote access usage, and
classroom exercises.


Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/emfacility/

Chairman, Educational Outreach subcommittee
promoting access to microscopes
Microscopy Society of America http://www.msa.microscopy.com/




From daemon Mon Jan 31 18:31:28 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Mon, 31 Jan 2000 15:43:33 -0800 (PST)
Subject: Question on Philips CM-12 SAD Alignment

Contents Retrieved from Microscopy Listserver Archives
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Hi there;

We have a new (to us) Philips CM-12 TEM in our lab and are wondering how
to get the intermediate lens focused on the diffraction aperture (for
making the first image plane and the diffraction aperture coincident
prior to obtaining a SAED pattern). It doesn't seem to be covered in the
manual.

Any help would be appreciated as this is a completely new microscope to
us.

Thanks,
Valerie Leppert



From daemon Mon Jan 31 18:31:30 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 31 Jan 2000 15:59:18 -0800
Subject: Re: SEM: JEOL JSM840-AM1000

Contents Retrieved from Microscopy Listserver Archives
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The JEOL 840 is an excellent instrument: very reliable & very easy to use.
Enjoy with confidence.

Earl Weltmer

P.S.: Does your friend need someone to install the SEM? I would love to install
it assuming it is in Hawaii.



Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, microscopists-
}
} A friend has the chance to buy a used JEOL JSM840 - AM1000 with EDX and
} all the bells and whistles. He has asked if this is a good instrument,
} and worth the price (whatever that is - he won't tell me). Since I
} haven't seen the instrument and I don't know JEOLs at all, I told him I
} would ask the experts.
}
} If anyone can tell me a little about the instrument and what it might be
} worth (the second part of the question being more difficult), I would
} appreciate it.
}
} In Honolulu it is gloriously clear and sunny, with temperatures near 80F
} during the day and about 60F at night, which is unusually cold but really
} nice.
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************



From daemon Mon Jan 31 18:31:31 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Mon, 31 Jan 2000 16:22:55 -0800
Subject: historesin supplier

Contents Retrieved from Microscopy Listserver Archives
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Hello All,
Does anyone know of a supplier for Historesin, formerly Cambridge, Leica,
LKB? And, the weather in SoCal is quite lovely today - it finally rained.
Thanks,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Mon Jan 31 20:32:35 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 1/31/00 11:06 AM
Subject: Sandbox Squabble-Weather

Contents Retrieved from Microscopy Listserver Archives
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The whole weather thing has been pretty silly.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


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Hi,

Are we like kids sitting in a sandbox and squabbling? Yes weather, no
weather! He said it first! She started it! I don't want weather on the
listserver. I want to read about weather in the boondocks. Who cares
about you, anyway? Sort of funny when you think of it that way, isn't it?

Bye guys,
Hildy Crowley






From daemon Tue Feb 01 07:28:29 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 31 Jan 2000 23:44:54 -0800 (PST)
Subject: Re: Wanted:Used TEM for virus work

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Would you be interested in a Zeiss 9s2 TEM? 60k is the top magnification.
It has been a nifty scope as we have upgraded to a Zeiss 109. If you are
interested let me know.
Cheers!
-Ken
------------
Ken Tiekotter
Dept. of Biol.
The University of Portland
5000 Willamette Blvd.
Portland, OR 97303

On Wed, 26 Jan 2000, Cynthia Shannon wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Subject: Wanted: Used TEM for virus work
} Date: Tues, 26 Jan 2000
} } From: cshannon-at-nctimes.net
} To: Microscopy-at-sparc5.microscopy.com
}
} Does anyone have an old TEM for virus work?
} I am the electron microscopist for the county veterinarian. We are short
} of funds. Please contact me by email.
} Thanks.
} Cindy Shannon
}
}
}



From daemon Tue Feb 01 07:28:46 2000



From: Asbj¿rn Skogstad :      asbjorsk-at-stami.no
Date: Tue, 01 Feb 2000 12:17:19 +0100
Subject: LM,fluorescence - aggregation of bacteria

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-Obviously an advantage for many bacteria species, but a nightmare for
the microscopist who wants to count
them. For several reasons we want to split the aggregates of bacteria
into single cells before counting.
We have tried mild detergent treatment and ultrasound though with
limited success. The samples are initially
taken, as filter samples in working atmospheres were bacteria could be
airborne, e.g. farm work. Afterwards
the bacteria are washed off the filter, resuspended, stained (AO),
refiltered on black pc-filter, mounted and
counted. Any suggestions for a treatment, which will de-aggregate the
bacteria, are most welcome.

Asbjorn Skogstad
National Inst. of Occup. Health,
Oslo, Norway
asbjorn.skogstad-at-stami.no



From daemon Tue Feb 01 07:40:49 2000



From: Tony Bruton :      bruton-at-EMU.UNP.AC.ZA
Date: 1/31/00 11:06 AM
Subject: Sandbox Squabble-Weather

Contents Retrieved from Microscopy Listserver Archives
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Chuck 'an all

That's why I tried to bring in a useful element to it by mentioning, a little tongue in cheek, my humidity vs formvar films question. But thanks to the silly weather thread I had some useful and interesting responses. May I be forgiven for saying that there's a silver lining..............

Tony Bruton
University of Natal
South Africa

} } } "Chuck Butterick" {cbutte-at-ameripol.com} 02/01/00 04:06AM } } }
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The whole weather thing has been pretty silly.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Hi,

Are we like kids sitting in a sandbox and squabbling? Yes weather, no
weather! He said it first! She started it! I don't want weather on the
listserver. I want to read about weather in the boondocks. Who cares
about you, anyway? Sort of funny when you think of it that way, isn't it?

Bye guys,
Hildy Crowley








From daemon Sun Feb 20 13:53:24 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Sat, 19 Feb 2000 16:42:48 -0500
Subject: DTSA Geometry setup for JEOL 2000FX in Experimental Header(Kevex

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Quantum)


Does anyone have the geometry settings for a Kevex Quantum detector on a
JEOL 2000FX? I am using DTSA and need the values. I would like to have the
sample to detector distance and the azimuthal angle. I am using +45 for the
azimuthal angle and 90 for the detector angle. Are these correct? I know
that the takeoff angle is 70.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Sun Feb 20 13:53:24 2000



From: David Lockwood :      d.lockwood-at-mailbox.uq.edu.au
Date: Sun, 20 Feb 2000 09:13:45 +1000
Subject: Fluoro - GFP, Nikon filters, fixation

Contents Retrieved from Microscopy Listserver Archives
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Hi all. I have just joined this list and am posting without the usual
"lurking time" due to time constraints - please forgive if recently
covered. I am also a novice in all microscopy techniques, especially
fluorescence.

I am trying to detect GFP-containing cells in liver tissue and having
some problems.

1. I can't determine the spectra for the filter blocks I am using (on a
Nikon Eclipse TE300 with TE-FM Epifluorescence attachment). The blocks
are marked "UV", "B-2" (blue from source, green in field) and "G" (green
from source, red in field). I have e-mailed Nikon and to be fair
they've only had a few days but I'm under some pressure. No help from
the manual. I have been using B-2 for GFP.

2. I get quite a lot of background fluorescence even with frozen
sections (fixed in neutral buffered formalin). Can anyone suggest a way
to reduce this? (eg any extra filters?)

3. I have read conflicting opinions on fixation. Most previous work has
used thick sections (50 microns cut eg with Vibratome, ? to avoid having
to embed tissue blocks). GFP is interfered with by acetone (and probably
other organic solvents) but I would have thought that after fixation
with formalin it should be stabilized and resistant to the
xylene/alcohol used in paraffin embedding. I have seen fluorescence
retained after this treatment but perhaps it can be improved.

4. For those with liver fluoro experience - on "UV" setting I see bright
fluorescence which photobleaches. I believe I am looking at retinoids in
stellate cells, although the bleaching is incomplete and a bit slower
than I have seen before. Can anyone confirm this?

Apologies for long post. Any help much appreciated.

David Lockwood
University of Qld Dept of Surgery
PA Hospital Brisbane Australia



From daemon Fri Feb 11 18:29:51 2000



From: Marty Reed :      mmr7001-at-humboldt.edu
Date: Fri, 04 Jan 1980 08:23:24 -0800
Subject: questions for MSA members

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A colleague would like your responses to these questions. I believe that I
say something on the first question about a year ago and apologize for
asking again.


} Here's what I/we need to know.
}
} 1) Are Microstar diamond knives equal to Diatome. We have an offer that
} will save us about $1200 on three knives ( one 3mm ultra with 45 degree
} angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).
}
}
} 2) Is the power controller feature on the Pelco Microwave Tissue processor
} (model #3451) worth it? (costs about $1300 additional compared to the
} Pelco 3450 without the power controller). I plan to do immunolocalization
} work, but am not sure this power controller feature is necessary or worth
} the cost.
}
} Casey
}
}
}
Thanks for your time
}
Marty Reed
Equipment Technician
Biology Department
Humboldt State University
Arcata CA 95521
707-826-3234
707-826-3201 FAX
mmr7001-at-axe.humboldt.edu







From daemon Tue Feb 01 10:56:26 2000



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Tue, 01 Feb 2000 10:58:08 -0400
Subject: TEM H600

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Hi!
Could anybody tell me what would be the sale price for a Hitachi
H600?
Thanks
Dorota



From daemon Tue Feb 01 10:56:32 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 1 Feb 2000 11:28:02 -0500
Subject: RE: Question on Philips CM-12 SAD Alignment

Contents Retrieved from Microscopy Listserver Archives
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I don't use a CM series microscope on a regular basis, but what is important
is that you set it up the same way every time.

You can not make the back focal plane of the objective lens coincident with
the objective aperture. The back focal plane is well above the location of
the objective aperture plane.

There are two ways that I use to set up diffraction patterns reproducibly
depending on whether I am using CBED or SAD. Both are set up after the
sample has been made eucentric and the image focussed.

CBED: This method can be done for both CBED and SAD. The shadow of the
condenser aperture defines the diameter of the diffraction disk. When the
intermediate lens is adjusted properly, the edges of the diffraction disks
will be in focus. You are grabbing the back focal plane for your
diffraction pattern in the projector lens system. You will note that all of
the HOLZ lines (if you can see them) are the sharpest at this condition. If
you have a highly polycrystalline sample with continuous diffraction rings,
this method is difficult to do.

SAD. Spread the beam with the condenser all the way. (clockwise in the
CM-12 will go to more parallel beam faster than CCW -I think.) Then focus
the spot to the smallest that you can. You can take a really long exposure
or cheat a little and put some intensity back into the pattern with the
condenser lens. You will note that the objective aperture is not focused in
this method.

The most important thing to remember is to make the sample eucentric and
focus before during either of these methods and to do the diffraction
focussing consistently from sample to sample.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Valerie Leppert [mailto:vjleppert-at-ucdavis.edu]
} Sent: Monday, January 31, 2000 6:44 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Question on Philips CM-12 SAD Alignment
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi there;
}
} We have a new (to us) Philips CM-12 TEM in our lab and are
} wondering how
} to get the intermediate lens focused on the diffraction aperture (for
} making the first image plane and the diffraction aperture coincident
} prior to obtaining a SAED pattern). It doesn't seem to be
} covered in the
} manual.
}
} Any help would be appreciated as this is a completely new
} microscope to
} us.
}
} Thanks,
} Valerie Leppert
}
}



From daemon Tue Feb 01 11:47:12 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Tue, 1 Feb 2000 09:02:04 -0800
Subject: Any other fluorescence microscopy classes?

Contents Retrieved from Microscopy Listserver Archives
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I received Simon Watkins' note about the microscopy class in Maine and
wonder if anyone knows about similar courses closer to California in the
near future? We've got a number of technicians working on fluorescence
microscopy in our lab, and I think it would be great to have some more
formal training for us!

Sincerely,
Laurie Wallin
UCSD Department of Anesthesiology
9500 Gilman Drive, 0629, La Jolla, CA 92093



From daemon Tue Feb 01 13:58:38 2000



From: Robert S Dotson :      rdotson-at-mailhost.tcs.tulane.edu
Date: Tue, 1 Feb 2000 11:55:19 -0600 (CST)
Subject: used Philips EM410

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We are disposing of our old Philips 410 transmission
electron microscope. It is in pretty good shape but
is not fully functioning. At minimum it needs its ion
getter reconditioned. Does anyone know of someone who
might want to buy it and fix it up or use it for
parts?
-Robert


____________________________________

Robert S. Dotson, Ph.D.,
Laboratory Supervisor, Microscopy

Coordinated Instrumentation Facility
605 Lindy Boggs Building
Tulane University
6823 St. Charles Avenue
New Orleans, LA 70118-5698

504-865-5142| fax 504-865-6768

rdotson-at-mailhost.tcs.tulane.edu
http://www.tulane.edu/~cif
____________________________________



From daemon Tue Feb 01 13:58:42 2000



From: best-at-Juniata.Edu
Date: Tue, 1 Feb 2000 14:09:48 -0500
Subject: RE: weather & Formvar films

Contents Retrieved from Microscopy Listserver Archives
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I am thankful to those who took this weather thread and imparted
some information that I can really use. The retired professor that taught
me how to release forvar films had no idea why sometimes it worked and
sometimes it didn't. Now at least I have a couple of likely variables to
check.

Thanks!
Chris Best
Mol. Biol.
Juniata College
Huntingdon, PA 16652


PS - I can't help but be amused that even on a forum for scientists,
the petty &/or silly items get the most responses. Tell the truth, do you
stay up at night watching Jerry Springer? (For heaven sake, don't answer
that!)




From daemon Tue Feb 01 14:23:32 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Tue, 01 Feb 2000 15:01:57 -0500
Subject: SAD and the back focal plane

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I am afraid that I do not agree with Scott Walck about back focal planes.
I do not have a CM 12 in the lab here. So I can not check that I am not
confusing the CM 12 with other Philips/FEI instruments. However, when
Scott says “You can not make the back focal plane of the objective lens
coincident with the objective aperture. The back focal plane is well above
the location of the objective aperture plane.” he is wrong. On all
microscopes except the very few which have very small pole-piece gaps for
high resolution, the objective aperture should coincide with the back focal
plane.

There is an easy and accurate way to find the true back focal plane on a CM
12 (or any other microscope with an immersion lens). Use a crystalline
sample, go to convergent-beam diffraction then use the diffraction focus to
make the Kikuchi lines as sharp as you can. That is the back focal plane.

If the microscope is set up properly, the image of the objective aperture
should be sharply in focus at nearly the same setting of the diffraction
focus. If the diffraction focus to give a sharp image of the objective
aperture is very different from the diffraction focus to make the Kikuchi
lines sharp, get your service engineer to reset the height of the objective
aperture until they agree.

If the sample is at the correct eucentric height, a selected-area
diffraction pattern in the true back focal plane will have sharp spots for
a C2 setting almost but not quite to the maximum (almost fully clockwise).
Again, if it does not, ask the service engineer to fix it.



Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvannia 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu



From daemon Tue Feb 01 15:14:30 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 1 Feb 2000 15:58:05 -0500
Subject: RE: SAD and the back focal plane

Contents Retrieved from Microscopy Listserver Archives
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Alwyn,
We have a CM-12 at our other facility and I will check it out in a day or
so.

I thought that I did and it worked like our JEOL 2000FX. In the 2000FX,
because the lens is highly excited, there are 3 cross overs in the objective
lens after the sample. That means the true back focal plane is inside the
objective lens and it is not possible to put the aperture at that plane. In
the 2000FX, I have focused the CBED pattern and have found the objective
aperture not in focus. I have also found that the two methods that I
outlined do not agree with the camera constants. The 2000FX has a condenser
mini lens to make the beam parallel, but the highly excited lens allows the
small probes. Since the CM-12 can form the small probes, I thought that the
lens system was working in a similar manner.

I will try it out unless someone beats me to it. How about it CM owners?

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Alwyn Eades [mailto:jae5-at-lehigh.edu]
} Sent: Tuesday, February 01, 2000 3:02 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: SAD and the back focal plane
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} I am afraid that I do not agree with Scott Walck about back
} focal planes.
} I do not have a CM 12 in the lab here. So I can not check
} that I am not
} confusing the CM 12 with other Philips/FEI instruments.
} However, when
} Scott says "You can not make the back focal plane of the
} objective lens
} coincident with the objective aperture. The back focal plane
} is well above
} the location of the objective aperture plane." he is wrong. On all
} microscopes except the very few which have very small
} pole-piece gaps for
} high resolution, the objective aperture should coincide with
} the back focal
} plane.
}
} There is an easy and accurate way to find the true back focal
} plane on a CM
} 12 (or any other microscope with an immersion lens). Use a
} crystalline
} sample, go to convergent-beam diffraction then use the
} diffraction focus to
} make the Kikuchi lines as sharp as you can. That is the
} back focal plane.
}
} If the microscope is set up properly, the image of the
} objective aperture
} should be sharply in focus at nearly the same setting of the
} diffraction
} focus. If the diffraction focus to give a sharp image of
} the objective
} aperture is very different from the diffraction focus to make
} the Kikuchi
} lines sharp, get your service engineer to reset the height of
} the objective
} aperture until they agree.
}
} If the sample is at the correct eucentric height, a selected-area
} diffraction pattern in the true back focal plane will have
} sharp spots for
} a C2 setting almost but not quite to the maximum (almost
} fully clockwise).
} Again, if it does not, ask the service engineer to fix it.
}
}
}
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvannia 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
}
}



From daemon Wed Feb 02 17:01:23 2000



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Tue, 1 Feb 2000 16:14:07 -0500
Subject: RE: Reichert Ultracut parts & repair

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-----Original Message-----



Jordi:
For Reichert repair and parts I would suggest Helmut Patzig of MOC
(Microscopical Optical Consulting Inc.), phone (914) 268-6450 or e-mail
MOCLeica-at-Aol.com Helmut is known to carry spare parts for Reichert and LKB
ultramicrotomes.
I have no vested interest in MOC other than as a satisfied customer.
If he cannot help, you could ask him what your Reichert transformer voltage
output should be and using a voltage meter adjust the voltage of a variable
voltage transformer to the correct amount. Make sure to incorporate a
"stop" on the dial so the voltage can't be accidentally moved above the
correct amount.
Henry


Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu


-----Original Message-----
------------------------------------------------------
On Mon, 24 Jan 2000, Marti, Jordi wrote:

} The power supply in our cryo microtome is having problems which might be
} related to the transformer. I was told by the service engineer that the
} transformer is no longer supported by Reichert. Does any one know where I
} can get a replacement ?
}
} Thanks
}
} Jordi Marti




From daemon Wed Feb 02 17:02:00 2000



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Wed, 02 Feb 2000 15:28:32 +1300
Subject: Looking for a turbo pump

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Does anyone have an extra turbo pump that they can part with?

the weather is quite wintery considering its summer here in Christchurch
New Zealand. low clouds with southerly prevailing winds (explaining the
COLD)




From daemon Wed Feb 02 17:02:10 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 02 Feb 2000 02:37:10 -0500
Subject: Silver membranes being discontinued

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hello,

I apologize in advance if this posting offends anyone. However there are a
good many people who use silver membranes in their work and to have the
supply from the worlds only manufacturer (to my knowledge) come to a halt,
has the potential of being highly disruptive at least to some programs:
=================================================
Osmonics has announced the closing of our Phoenix, AZ manufacturing facility
effective 1 May 2000. Since our silver membranes are manufactured in this
facility, Osmonics has decided to end production of this membrane because of
declining sales and the very expensive costs associated with moving the mfg.
. plant to another location. All orders placed before 1 March, 2000 will be
honored and filled.
==================================================
We plan to make a "last buy" before the cut off date of March 1, 2000. I
would advise anyone depending on these silver membranes for their work to
take stock of their future requirements because after the cut off date,
sales will be possible only from remaining stocks.

For those who do run out, and are in a bind, we are prepared help them find
alternative filtration media, of which there are indeed some alternatives
for some applications.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Feb 02 17:02:13 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 02 Feb 2000 08:35:01 +0000
Subject: LM - Cryostat - marine larvae

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers

I have someone here who wants to freeze and cut cryostat sections of
marine larvae (5 microns?) for immuno/light microscopy. The specimens
are 100-200 microns in length.

1. What would be the best way of handling these small items?
2. What would be the best support/medium e.g TissueTek?
3. What about cryoprotection? I am familiar with 2.3 molar sucrose
for EM.
4. Any general tips for immuno (protein/amino peptidases)?

Thanks - Keith
_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Wed Feb 02 17:02:11 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 2 Feb 2000 08:38:24 +0000 (GMT Standard Time)
Subject: re - CM12 SAD focus

Contents Retrieved from Microscopy Listserver Archives
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Hi Valerie,

Like the other respondees I am not a CM12 user
(where are they?), however, as an ex CM12 user of some 10
years ago I think that I remember the alignment that Valerie
is asking about. In the basic alignment of the machine there
was a step during which the SAD aperture was focussed. I
think that it was in the `service calibrations' page. This
may have changed in later software revisions.

The alignment of this microscope was usually
carried out by the engineer when the instrument was
installed. They would set up the objective lens current and
adjust the specimen goniometer height to ensure that when
it was at the eucentric position it was correctly in focus.
Following this a complete column alignment was carried out
including focussing the SAD aperture. The manufacturers
then assumed that the operator would set up the specimen to
the eucentric height, it should focus in the same position
and the aperture should be in focus.

If the aperture is not in focus when in the SA
range (as noted next to the mag readout) then check you are
correctly at the eucentric position, if you are then either
it was not aligned properly after installation or something
has changed.
NOTE: DO NOT ADJUST THE SERVICE ALIGNMENTS UNLESS YOU KNOW
WHAT YOU ARE DOING AS YOU CAN LOSE THE INSTRUMENT
ALIGNMENTS.

Good luck,
Ron


----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Feb 02 17:02:14 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Wed, 02 Feb 2000 11:20:15 +0100
Subject: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
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I got a tip for finding the condenser lens settings required for parallel
illumination from Angus Kirkland (Cambridge).

Make sure you are in microprobe mode and set up for SAED and the specimen
is out of the way. Spread the beam, put in the SA Aperture, go to
diffraction and then sweep the SA aperture (SAA) from side to side and
minimise the deflection of the diffraction spot by tweaking the
illumination control (C2 or second condenser lens). A little bit of thought
will convince you that anything other than a parallel beam will give you a
side to side motion (tilt) when the SAA is swept.

I have found it useful to keep a table of C2 condenser lens current
settings for each spot size (C1 excitation) in microprobe mode. Setting the
C2 lens for parallel illumination and adjusting the diffraction focus to
get the sharpest spot will then give you near perfect SAD conditions.

Anyone contemplating electron holography for example, will have to start
from the parallel illumination to get the best spatial coherence for their
holograms.

********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Wed Feb 02 17:02:20 2000



From: Matt_Plantinga-at-amway.com
Date: Wed, 2 Feb 2000 08:15:48 -0500
Subject: LSCM Need help with non-fluorescing resin

Contents Retrieved from Microscopy Listserver Archives
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I am working on fluorescent imaging of thin sections cut from samples
embedded in TEM embedding resin, and am having a significant problem with
resin fluorescence. Does anyone know of an embedding resin that doesn't
autofluoresce?

Matt Plantinga
Amway Corporation
matt_plantinga-at-amway.com



From daemon Wed Feb 02 17:03:28 2000



From: Andrew Ochalski :      aochalsk-at-science.uottawa.ca
Date: Wed, 2 Feb 2000 14:05:21 GMT+5
Subject: Heating stage with DvorakStotler Chamber...Recommendations?

Contents Retrieved from Microscopy Listserver Archives
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{color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Arial {/param} {smaller} Fellow Microscopists,


We have a Zeiss Axiophot microscope and a
Dvorak-Stotler Chamber that we would like to
assemble into a temperature-controlled environment
for digital live-cell imaging. I have several questions
about the best way to combine these elements:

i) Can anyone recommend a heating stage
compatible with both the microscope and the
chamber?

ii) Would the best position for a temperature sensor
be on the top (near the objective?) or on the bottom
of the chamber, or both (two sensors)?

iii) Is it better to heat the media/fluids in their
containers, or to have an in-line heater ( brand
recommendations?)

iv) Are there any fuid flow or temperature
considerations specific to this chamber that need to
be addressed (gravity vs. pump feed, shear forces,
heating stage redundant, etc.)?

v) Are there any problems related to objective lens
mag/NA that need to be overcome when using this D-
S apparatus?


Any references, anecdotes, or words of wisdom
would be appreciated. Dealers, if you have a product
that you think I could use, please don't hesitate to
contact me. Thank you in advance. {FontFamily} {param} BrushScript BT {/param} {bigger}

{nofill}
Regards,

Andrew Ochalski,
Microscopy Technician,
Dept. of Biology,
University of Ottawa
Room 108, Gendron Bldg.
130 Louis Pasteur,
Ottawa, Ontario
CANADA
K1N 6N5
613-562-5800 x6343
FAX: 613-562 5486

{/x-rich}



From daemon Wed Feb 02 17:02:50 2000



From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Wed, 02 Feb 2000 09:32:01 -0500
Subject: Looking for a new microscope

Contents Retrieved from Microscopy Listserver Archives
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I would like to get some information from some of the top companies on
microscopes with photographic and possibly fluourescent capabilities.
Please email me or call 313-993-4195
Thank you

Cheri Owen
Wayne State University



From daemon Wed Feb 02 17:02:48 2000



From: BARRY SHAW :      Barry.Shaw-at-nottingham.ac.uk
Date: Wed, 2 Feb 2000 14:36:36 GMT0BST
Subject: Re: Any other fluorescence microscopy classes?

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

Not really a reply but another question!!
Anybody know of a similar course a little further east - England
(Great Britain) to be exact !!?
Thanks in Advance,

Baz


Barry Shaw
Senior Imaging Technician
Molecular & Cell Biology
E Floor
School Of Biomedical Sciences
University of Nottingham Medical School
NG7 2UH



From daemon Wed Feb 02 17:02:49 2000



From: Manfred Prantl :      prantl-at-alicona.com
Date: Wed, 02 Feb 2000 15:42:52 +0100
Subject: SEM and 3D?

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We are a company that has specialized on extracting 3D information
from stereoscopic SEM images. Currently we try to figure out the
potential applications and the future prospects of such techniques for
the microscopy community.

What we do exactly is that we take two images from a sample on the
SEM from slightly different tilt angles and use digital image processing

to compute a 3D elevation model. From there you can then perform
various analysis steps like extraction of 3D profiles, determination
of surface roughness etc.

My questions now are:

1.) How would you judge the importance of obtaining 3D data from a SEM
image?
2.) What would be the major requirements for such a data set to be
useful
(like density, accuracy, number of false alarms, etc.)?
3.) Is it of importance to you that you get the 3D data and the image
data
together and not separated (like it is the case with AFM)?
4.) How would you judge the future prospects and influence of such a
technique on your personal field of work?
5.) Do you generally trust the measurement of 3D data via stereoscopic
images?
6.) Do you already have experience with stereoscopic depth measurments
and what are your conclusions?

Best regards, Manfred Prantl
R&D
Alicona GmbH, Germany
www.alicona.com



From daemon Wed Feb 02 17:02:53 2000



From: Russell E. Cook :      cook-at-horus.et.anl.gov
Date: Wed, 2 Feb 2000 09:15:18 -0600
Subject: Back focal plane of CM's

Contents Retrieved from Microscopy Listserver Archives
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Irene Piscopo of FEI/Philips probably can answer your question. I'm copying
this message to her although if she's in the "field" a response may take a
few days. Also, Max Otten of FEI/Philips is another good source for that
type of information.

We have a CM-120 so we're aware of your problems in deciphering the
operator's manual. Good luck!

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124

(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-nola.srrc.usda.gov

-----Original Message-----
} From: Valerie Leppert [mailto:vjleppert-at-ucdavis.edu]
Sent: Monday, January 31, 2000 5:44 PM
To: Microscopy-at-sparc5.Microscopy.Com


I can't speak about Philips CM12's, but the back focal plane of the
objective coincides with the objective aperture in the CM30 here.

----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
cook-at-horus.msd.anl.gov




From daemon Wed Feb 02 17:03:04 2000



From: Piscopo, Irene :      IPiscopo-at-FEICO.COM
Date: Wed, 2 Feb 2000 07:59:45 -0800
Subject: RE: Question on Philips CM-12 SAD Alignment

Contents Retrieved from Microscopy Listserver Archives
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Hello all: Valerie Leppart, you can reach me at 203-853-3256. I cannot call
you since there is no phone number to contact. Irene Piscopo

The CM 12 microscope has four mag ranges: LM, M, SA, Mh

As long as you are eucentric and using the SA range the image plane
and the diffraction aperture plane will be in focus. (It is done
automatically by the instrument.) This will give you accurate SAD down to
1um. The objective mag in the plane of the diffraction aperture is
approxmately 27X. If you wish to obtain diffraction from areas smaller than
one micron, use uD, or uuD. If you call me I will discuss these methods
with you and send you detailed instructions on using these methods.


Irene Piscopo


} -----Original Message-----
} From: Ingber, Bruce F. [SMTP:bingber-at-commserver.srrc.usda.gov]
} Sent: Wednesday, February 02, 2000 10:14 AM
} To: Microscopy-at-MSA. Microscopy. com (E-mail)
} Cc: Piscopo Irene (CS) (E-mail); Max T. Otten (E-mail)
} Subject: FW: Question on Philips CM-12 SAD Alignment
}
} Irene Piscopo of FEI/Philips probably can answer your question. I'm
} copying
} this message to her although if she's in the "field" a response may take a
} few days. Also, Max Otten of FEI/Philips is another good source for that
} type of information.
}
} We have a CM-120 so we're aware of your problems in deciphering the
} operator's manual. Good luck!
}
} Bruce F. Ingber, Biologist
} USDA-ARS, SRRC
} 1100 Robert E. Lee Blvd.
} New Orleans, LA 70124
}
} (504) 286-4270 phone
} (504) 286-4419 fax
} bingber-at-nola.srrc.usda.gov
}
} -----Original Message-----
} From: Valerie Leppert [mailto:vjleppert-at-ucdavis.edu]
} Sent: Monday, January 31, 2000 5:44 PM
} To: Microscopy-at-sparc5.Microscopy.Com
} Subject: Question on Philips CM-12 SAD Alignment
}
} ------------------------------------------------------------------------
} The Microscopy ListServer-Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there;
} We have a new (to us) Philips CM-12 TEM in our lab and are wondering how
} to
} get the intermediate lens focused on the diffraction aperture (for making
} the first image plane and the diffraction aperture coincident prior to
} obtaining a SAED pattern). It doesn't seem to be covered in the manual.
} Any help would be appreciated as this is a completely new microscope to
} us.
} Thanks,
} Valerie Leppert



From daemon Wed Feb 02 17:03:59 2000



From: rgriffin-at-eng.uab.edu
Date: Wed, 2 Feb 2000 14:15:55 -0600
Subject: Gray level

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How many gray levels can the human eye distinguish? We've found several
references that disagree.
If anyone knows of a reference - that would be best.


Robin Griffin
UAB



From daemon Wed Feb 02 17:04:02 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 02 Feb 2000 16:07:50 -0500
Subject: Re: Gray level

Contents Retrieved from Microscopy Listserver Archives
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Robin,

I don't have the reference right in front of me, but I believe that
Gonzalez and Wintz say that

1. The eye can respond to an intensity range of ~10^10 in light intensity,
but this is the adaptive response. The perceived brightness is a log (some
will argue power law) function of the incident intensity.

2. In any one point in an image, the eye can distinguish at most ~20-30
gray levels... But... in a complex image you need at least 100 gray levels
for the eye to see it as smooth. In other words, the eye seems to adapt as
it scans the image.

Cheers,
Henk


At 02:15 PM 2/2/00 -0600, rgriffin-at-eng.uab.edu"-at-sparc5.Microscopy.Com wrote:

} How many gray levels can the human eye distinguish? We've found several
} references that disagree.
} If anyone knows of a reference - that would be best.
}
}
} Robin Griffin
} UAB

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Murphy's Law: "If anything can go wrong, it will."
Commentary: "Murphy was an optimist."



From daemon Wed Feb 02 18:52:13 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Wed, 2 Feb 2000 15:04:41 -0800 (PST)
Subject: Re: re - CM12 SAD focus

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Thanks for your reply and everyone else's reply regarding the SAD
alignment. There seem to be a lot of different opinions about how this
done!

We did set the sample at the eucentric and did notice that the SAD
aperture is not quite in focus when in image mode. Of course this will
cause the diffraction information to come from an area that is displaced
with respect to the aperture position.

I am accustomed to being able to independently focus the intermediate lens
on the SAD aperture, and then bring the image into focus using the
objective lens - making the image plane and the SAD aperture coincident.
This is on a much older Philips model and a much older Hitachi model.

However, on the CM-12, there doesn't seem to be a way for the user to do
this in normal operation. I've tried a few of the suggestions (haven't
worked my way through them all yet!) with no luck yet.

It does seem that this adjustment has been grouped under the category of
"service alignment" in newer models.

Thank you to everyone who has replied.

Valerie Leppert




From daemon Wed Feb 02 18:52:14 2000



From: Karl Hagglund-KW :      hagglund.kw-at-pg.com (by way of Nestor J. Zaluzec)
Date: Wed, 2 Feb 2000 17:06:39 -0600
Subject: LM Sony camera bayonet mount

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We have a sony DXC-760 MD ccd camera that we have mounted to a standard camera
lens with a bayonet adapter. Unfortunately, we have borrowed the adapter and
have to return it. Is there a standard size (i.e. Nikon, Minolta, Canon) lens
adapter that works with this camera mount? We can't find a part number or
description for the adapter, and the book doesn't describe the size. We have
already ordered and returned one that didn't fit.

Thanks in advance.

Karl Hagglund
Proctor and Gamble
Food and Beverage Analytical Microbiology




From daemon Wed Feb 02 18:52:16 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk (by way of Nestor J. Zaluzec)
Date: Wed, 2 Feb 2000 17:14:03 -0600
Subject: Electron diffraction simulations

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Fellow Listers,
Does anyone know of a (preferrably free) software package
which simulates ray paths, including the diffraction mode,
in the TEM? We would like to use such a package to teach
physics students a bit of optics.

Thanks,
Eric



----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk




From daemon Wed Feb 02 18:52:20 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 02 Feb 00 16:29:17 -0800
Subject: RE: LSCM Need help with non-fluorescing resin

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: RE: LSCM Need help with non-fluorescing resin
Dear Matt,

Try any of the immunocytochemical resins (Lowicryl, LR White or Gold, Unicryl etc). When specific antibodies are used on embedded tissue, they produce very beautiful fluorescent patterns that look like line drawings.

Paul Webster

Matt_Plantinga-at-amway.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} I am working on fluorescent imaging of thin sections cut from samples
} embedded in TEM embedding resin, and am having a significant problem with
} resin fluorescence. Does anyone know of an embedding resin that doesn't
} autofluoresce?
}
} Matt Plantinga
} Amway Corporation
} matt_plantinga-at-amway.com
}
}
}
}
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Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Feb 02 22:19:11 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 2 Feb 2000 19:56:04 EST
Subject: Re: Gray level

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In a message dated 2/2/00 4:00:26 PM,
rgriffin-at-eng.uab.edu-at-sparc5.Microscopy.Com writes:

} How many gray levels can the human eye distinguish? We've found several
}
} references that disagree.
}
} If anyone knows of a reference - that would be best.

Most of the books on image processing say the same thing (probably all copied
from each other and other non-prime sources), but I don't know of an original
reference based on real research. You can find the same ideas in books like
Frisby "Seeing: illusion, brain and mind", Oxford, 1980; Marr "Vision",
Freeman, 1982; and Rock "Perception", Freeman, 1984 which deal with the
human visual system not in the context of computer graphics. These are
probably closer to the original research.

The basic idea is that it takes about a 2-2.5% change in absolute brightness
(i.e. a ratio of 1.025) to be just detectable, and only if it is a fairly
sudden change spatially (or temporally for that matter). That is where the
20-30 distinct brightness levels over the full range of brightness that can
be seen at one time (i.e. without accommodations like varying pupil size)
comes from. The requirement that an entire scene have about 100 levels to
avoid banding is more controversial, based on the idea that the eye can
perform some accommodation as it traverses from one part of a scene to
another. This probably doesn't apply when (e.g.) looking through a
microscope, so the 20-30 number would be more applicable. It also ignores
color, which complicates things further.



From daemon Wed Feb 02 22:19:14 2000



From: special123-at-smartportfolio.com
Date: Thu, 3 Feb 2000 12:17:58 +0900
Subject: TEM:finding the parallel beam

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Dear Jonathan,

} From what is my understanding of optics - this will not give you parallel
illumination at the specimen plane (if this what you meant?).
By tweaking C2 you are moving the crossover plane (which is actually the
diffraction plane). You can in the same way make the spot not to move by
changing the diffraction focus. For each setting of C2 you can find the
crossover by changing the diff. focus.
Try this - spread the beam, go to diffraction, focus by diffraction focus
for smallest spot, go back to imaging mode put the SAA, go to diffraction
and try the procedure you described. I think the spot will not move.
Ofcourse if you expand the beam too much you can no longer produce small
diffraction spot (without the use of SAA) because you go out of paraxial
mode.
The diffraction plane is always the crossover plane not the theoretical back
focal plane of the objective. Its z-position changes with the change of the
C2 excitement.
The spatial coherency of the electron waves is very high (actually the
electrons are flying one by one through the microscope). The limiting
factors are the source size, the illumination angle and the energy spread.

Please correct me if I'm wrong ... I'm still "green" in electron microscopy
.. I'll be happy to learn from experienced users.

Best wishes,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Jonathan Barnard {Jonathan.Barnard-at-Angstrom.UU.SE}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, February 02, 2000 7:20 PM


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From daemon Thu Feb 03 15:56:54 2000



From: Dmitri V. Sokolov :      sokolov-at-ryouko.rciqe.hokudai.ac.jp
Date: Thu, 3 Feb 2000 20:20:06 +0900
Subject: JAMP-7810 manual in English and local chemical analysis

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Dear all,

We are making preparations for experiments on determination of the chemical
composition of semiconductor oxides, formed locally. The size of the
modified area should be about 1 micron, and we are not sure yet if it is
possible to make mentioned chemical analysis with Auger microprobe at all.

Another problem is that our newly came Auger spectroscope JAMP-7810 has no
English manual with it. And believe me, it is barely possible to read that
in Japanese!

We would be very grateful for your suggestions on both my questions:
- how to measure the best chemical bonding on the spot with 1x1 sq.
micrometer dimensions
- where to get the manual (or copy of it) from.
Remark: Mails to USA and Japanese representatives of JEOL gave no result!

Best regards.
Dmitri


__________________________________________
Dmitri V. Sokolov, Doctor Course student
Research Center for Interface Quantum Electronics,
Hokkaido University, North 13, West 8, Kitaku,
Sapporo 060, Hokkaido, Japan
Phone 81-11-706-7174 Fax 81-11-716-6004
http://www.geocities.com/SiliconValley/Campus/1314
AOL Instant Messenger FalconDot
ICQ 9418072
__________________________________________




From daemon Thu Feb 03 15:57:12 2000



From: bengi :      bengi-at-unimo.it
Date: Thu, 3 Feb 2000 14:54:20 +0100
Subject: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,
I have started a research project on ammonium -bearing mineral (zeolites),
and now I'm trying to quantify N using electron microprobe analysis. My
problems of dealing with N are:

a) The thickness of the coating (in the literateur = approximately 150 A¡).
Does anyone have information on the model of coater to making these films?
Does anyone have experience with preparation of such
samples?

b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
standards for such a sample ?

Any other suggestions about quantitative nitrogen analysis would be greatly
appreciated !!
Thanks for your time.

My best regards

Eugenia


Eugenia Marchi
Universitˆ degli Studi di Modena
Dip. Scienze della Terra
P.le S.Eufemia n¡.19
41100 Modena (Italy)
Tel. +39-059-417289
Fax. +39-059-417399
e-mail: bengi-at-unimo.it




From daemon Thu Feb 03 15:57:36 2000



From: Barr, Dennis B :      dennbarr-at-eastman.com
Date: Thu, 3 Feb 2000 09:30:07 -0500
Subject: RE: Silver membranes being discontinued

Contents Retrieved from Microscopy Listserver Archives
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It's unfortunate that this manufacturer is stopping production. Aren't
there other manufacturers?

Dennis B. Barr (dennbarr-at-eastman.com)
Physical Chemistry Research Laboratory
Physical & Analytical Chemistry Research Division
Eastman Chemical Company
Kingsport, TN 37662-5150

B-150B, R-132E, (423) 229-2188


} -----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
} Sent: Wednesday, February 02, 2000 2:37 AM
} To: MICROSCOPY BB
} Subject: Silver membranes being discontinued
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Hello,
}
} I apologize in advance if this posting offends anyone. However there are a
} good many people who use silver membranes in their work and to have the
} supply from the worlds only manufacturer (to my knowledge) come to a halt,
} has the potential of being highly disruptive at least to some programs:
} =================================================
} Osmonics has announced the closing of our Phoenix, AZ manufacturing
} facility
} effective 1 May 2000. Since our silver membranes are manufactured in this
} facility, Osmonics has decided to end production of this membrane because
} of
} declining sales and the very expensive costs associated with moving the
} mfg.
} .. plant to another location. All orders placed before 1 March, 2000 will
} be
} honored and filled.
} ==================================================
} We plan to make a "last buy" before the cut off date of March 1, 2000. I
} would advise anyone depending on these silver membranes for their work to
} take stock of their future requirements because after the cut off date,
} sales will be possible only from remaining stocks.
}
} For those who do run out, and are in a bind, we are prepared help them
} find
} alternative filtration media, of which there are indeed some alternatives
} for some applications.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================



From daemon Thu Feb 03 15:57:55 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 3 Feb 2000 08:11:02 -0700
Subject: RE: Gray level

Contents Retrieved from Microscopy Listserver Archives
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Yes, I agree with John. A better question than "how many gray levels can
one see" is probably "how many gray levels can one see under what
circumstances". The answer is probably very different for a static image
and a dynamic image, with edges or without, in low light conditions or
bright light conditions.

The human eye (and brain) has had Millions of years to evolve and
develop some rather sophisticated algorithms to deal with different
conditions and the answer is probably also very complex. For example,
our vision is extremely good at detecting movement. On a completely
static image this tends to depress differences, while on a dynamic image
it tends to enhance differences (we automatically focus on the changes).


Maybe you can test that yourself:

take the same image on a computer at different bit depths (=levels of
gray). Then randomly put them on the screen (without watching the
change) and try to decide, which image it is. Then do the same but watch
the changes and try to decide how many gray levels you need before the
changeover becomes invisible. You could do this with a "noise" image, an
image that shows some object, and a smooth gray wedge. I for one would
be interested to hear about the results.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
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===================================
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} ----------
} From:
"DrJohnRuss-at-aol.com"-at-sparc5.Microscopy.Com[SMTP:"DRJOHNRUSS-at-AOL.COM"-at-SPA
RC5.MICROSCOPY.COM]
} Sent: Wednesday, February 02, 2000 5:56:04 PM
} To: Microscopy-at-sparc5.Microscopy.Com; rgriffin-at-eng.uab.edu
} Subject: Re: Gray level
} Auto forwarded by a Rule
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In a message dated 2/2/00 4:00:26 PM,
rgriffin-at-eng.uab.edu-at-sparc5.Microscopy.Com writes:

} How many gray levels can the human eye distinguish? We've found
several
}
} references that disagree.
}
} If anyone knows of a reference - that would be best.

Most of the books on image processing say the same thing (probably all
copied
from each other and other non-prime sources), but I don't know of an
original
reference based on real research. You can find the same ideas in books
like
Frisby "Seeing: illusion, brain and mind", Oxford, 1980; Marr "Vision",

Freeman, 1982; and Rock "Perception", Freeman, 1984 which deal with the

human visual system not in the context of computer graphics. These are
probably closer to the original research.

The basic idea is that it takes about a 2-2.5% change in absolute
brightness
(i.e. a ratio of 1.025) to be just detectable, and only if it is a
fairly
sudden change spatially (or temporally for that matter). That is where
the
20-30 distinct brightness levels over the full range of brightness that
can
be seen at one time (i.e. without accommodations like varying pupil
size)
comes from. The requirement that an entire scene have about 100 levels
to
avoid banding is more controversial, based on the idea that the eye can
perform some accommodation as it traverses from one part of a scene to
another. This probably doesn't apply when (e.g.) looking through a
microscope, so the 20-30 number would be more applicable. It also
ignores
color, which complicates things further.



From daemon Thu Feb 03 15:57:56 2000



From: MICHAEL MOHN :      MMOHN-at-mail.monroe.cc.mi.us
Date: Thu, 03 Feb 2000 10:14:13 -0500
Subject: Re: LM Sony camera bayonet mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most industrial cameras use C-mounts. C-mount to camera lens adapters are available from Edmund Industrial Optics (http://www.edmundoptics.com phone:800-363-1992) on page 165 of their 2000 catalog. They have mounts for several types of cameras, and list for $62 each. If your connecting the camera to a 'scope, Edmund sells a relay lens for $240 which mounts the camera to a 23mm eyepiece tube. For connecting to trinocular and CCD ports on a scope, Diagnostic Instruments sells relay lenses for many types of 'scopes (http://www.diaginc.com) for $299.


Michael Mohn
Assistant Professor of Materials Technology
Monroe County Community College
Phone: 734-384-4122 Fax: 734-242-9711
http://www.monroe.cc.mi.us/mmohn

} } } "Karl Hagglund-KW" by way of Nestor J. Zaluzec {hagglund.kw-at-pg.com} 02/02 6:06 PM } } }
We have a sony DXC-760 MD ccd camera that we have mounted to a standard camera
lens with a bayonet adapter. Unfortunately, we have borrowed the adapter and
have to return it. Is there a standard size (i.e. Nikon, Minolta, Canon) lens
adapter that works with this camera mount? We can't find a part number or
description for the adapter, and the book doesn't describe the size. We have
already ordered and returned one that didn't fit.

Thanks in advance.

Karl Hagglund
Proctor and Gamble
Food and Beverage Analytical Microbiology






From daemon Thu Feb 03 15:57:57 2000



From: rlvaughn-at-unmc.edu
Date: Thu, 3 Feb 2000 09:39:47 -0600
Subject: Re: Gray level

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Are you equating brightness levels with grey levels here?
I was told something like this years ago when we were buying our first
digital B&W camera, when I asked the vendor about the competitors thousand
plus grey levels vs his 256 grey levels and was told that the eye could not
distinguish greater levels. Now, four or five years later everyone is
pitching 10, 12 and 16? bit cameras, and yet the images from these cameras
do look better. Is it the bits and increased grey levels? I know that if
your doing image analysis at the pixel level that more bits are to your
advantage but what about just photgraphic quality?

Rick Vaughn



From daemon Thu Feb 03 15:58:08 2000



From: L. D. Marks :      ldm-at-apollo.numis.nwu.edu
Date: Thu, 3 Feb 2000 10:06:36 -0600 (CST )
Subject: Re: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, but I think we are getting a little confused here.

1) The diffraction plane is (by definition) the objective lens
focal length below the sample - it is the focus of parallel
directions coming from the sample. The focal length (and
parameters such as Cs, Cc) change rapidly with the objective lens
current so an eucentric position makes life simple but is not
necessarily the best condition to use. (For HREM you generaly
want to have the objective lens stronger and reduce Cs.)

2) In general C2 does not change the focal length at all. You
can have some effect from coupling of the magnetic fields, but
all C2 is supposed to do is change the convergence angle.

3) The objective aperature is (approximately) in the back-focal
plane, given that it is finite and height adjustments are only
done coarsely (for a specific value of the objective current
only).

4) The selected area aperature is (approximately) in the same
plane as the object for the same reasons as above. Remember that
there are positional errors in SA diffraction due to Cs (see
Hirsch et al for instance).

5) You should focus Kikuchi-lines, not go for the smallest spot,
since these are real object in the diffraction pattern. What you
will see is then representative of you incident beam, sometimes
a Gaussian range of directions.

6) The simplest way to get more parallel illumination in general is
to reduce the condensor aperture size.

7) All bets are off if you have a FEG. Rather than having incoherent
illumination things like the Cs of the prefield (above the sample)
and postfield (below) add coherently making it much more complicated.
For instance, the illumination angle varies across the field of view.
While this is also there with LaB6, it is a weak effect (except for
certain classes of diffracted beams, e.g. those with a screw-axis). With
a FEG you can get a "spot" pattern many ways, and most of these will have
large aberrations (e.g. pincushion).

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Thu Feb 03 15:58:11 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Thu, 3 Feb 2000 16:24:08 +0000 (GMT)
Subject: Re: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eugenia,
the microprobe is not the best tool for the analysis of
ammonia in zeolites because ammonia is very volatile in the
electron beam. If your zeolite is phase pure it would be
far better to dissolve it and do a classical chemical
analysis.

regards,
Eric
On Thu, 3 Feb 2000 14:54:20 +0100 bengi {bengi-at-unimo.it}
wrote:


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear listers,
} I have started a research project on ammonium -bearing mineral (zeolites),
} and now I'm trying to quantify N using electron microprobe analysis. My
} problems of dealing with N are:
}
} a) The thickness of the coating (in the literateur = approximately 150 A°).
} Does anyone have information on the model of coater to making these films?
} Does anyone have experience with preparation of such
} samples?
}
} b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
} N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
} standards for such a sample ?
}
} Any other suggestions about quantitative nitrogen analysis would be greatly
} appreciated !!
} Thanks for your time.
}
} My best regards
}
} Eugenia
}
}
} Eugenia Marchi
} Università degli Studi di Modena
} Dip. Scienze della Terra
} P.le S.Eufemia n°.19
} 41100 Modena (Italy)
} Tel. +39-059-417289
} Fax. +39-059-417399
} e-mail: bengi-at-unimo.it
}
}
}

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk





From daemon Thu Feb 03 15:58:23 2000



From: L. Paul Bédard :      Paul_Bedard-at-uqac.uquebec.ca
Date: Thu, 3 Feb 2000 12:12:17 -0500
Subject: Need repairman for Hitachi SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I need somebody to repair a Hitachi SEM S-2700.
Please contact me individually not the list.
Thanks for help,
--
L.Paul Bédard, ing. Ph.D.
Resp. Lab. Géochimie | Lab. Manager
Sciences Appliquées ; Université du Québec à Chicoutimi Canada
Tél. : 418/545-5011 x 2276 | Alt. E-mail : pbedard-at-saglac.qc.ca
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
La période humaine, depuis la création d'Adam jusqu'à nos jours, n'est qu'une
moisissure dans l'histoire de notre planète
JCK Laflamme 1886



From daemon Thu Feb 03 15:58:37 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 03 Feb 2000 11:22:46 -0600
Subject: Re: Gray level

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Let me offer my guess that the improved appearance of the images is be due
largely to the improved signal-to-noise ratio in the newer cameras.

Of course there is also something to be gained in the dynamic range by the
extra bits of resolution. They are much more forgiving and allow
substantial changes in contrast and brightness without sacrificing the
information contained in the data.

Warren S.

At 09:39 AM 2/3/2000 -0600, you wrote:

} Are you equating brightness levels with grey levels here?
} I was told something like this years ago when we were buying our first
} digital B&W camera, when I asked the vendor about the competitors thousand
} plus grey levels vs his 256 grey levels and was told that the eye could not
} distinguish greater levels. Now, four or five years later everyone is
} pitching 10, 12 and 16? bit cameras, and yet the images from these cameras
} do look better. Is it the bits and increased grey levels? I know that if
} your doing image analysis at the pixel level that more bits are to your
} advantage but what about just photgraphic quality?
}
} Rick Vaughn



From daemon Thu Feb 03 15:58:28 2000



From: Michael Plociniak :      plocinia-at-aecom.yu.edu
Date: Thu, 03 Feb 2000 12:44:23 -0500
Subject: New Gold Enhancement Reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,
Has anyone used gold enhancement reagents from Nanoprobes, Inc.
(Stonybrook, NY - USA) as an alternative to silver enhancement for
enlarging 1 nm immunogold probes?

Advertisements state that gold enhancement has lower background and is
compatible with osmium. Can anyone verify this from personal experience?

In addition to these advantages, I am hoping that milder pH conditions will
be less damaging to ultrastructure in cultured neurons (using a
preembedment protocol).

Thank you,
Michael



From daemon Thu Feb 03 15:58:41 2000



From: Sinkler, Wharton :      wharton.sinkler-at-anlw.anl.gov
Date: Thu, 03 Feb 2000 12:13:10 -0700
Subject: Re: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rado,

} The diffraction plane is always the crossover plane not the theoretical back
} focal plane of the objective. Its z-position changes with the change of the
} C2 excitement.

Your statement above is not wrong, but isn't the standard TEM viewpoint (or
terminology). Yes, the plane at which the spot is sharpest (the in focus
plane for the demagnified filament image) is the crossover plane. However
to generally call this the "diffraction plane" is confusing. For example in
the case of CBED, the crossover plane has moved (up or down, depending on
whether C2 was initially under or overfocused) to the specimen plane. This
doesn't make the specimen plane the "diffraction plane". Rather, the
"diffraction plane" is now considered the in-focus plane for the C2
aperture. This is at the objective lens back-focal plane - the plane at
which points on the specimen are magnified to infinity and where the
position variable corresponds to the illumination angle. In collecting an
SADP you can correct for slightly non-parallel illumination by correcting
diffraction focus slightly off of the true back focal plane, but this should
be only slight.

What was proposed in the original mail was a technique of determining how
parallel the incident beam is, and of improving things by tweaking C2. If
the beam isn't very parallel, you'll see the diffraction spots shift when
you move the SA aperture. This is because the incident beam inclination
depends on the position on the sample.

I would think the "tweak" to C2 for obtaining more parallel illumination
would always in the direction of greater beam spread (unless I am
misunderstanding something?).

Regards,
Wharton
++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler
Argonne National Laboratory West
P. O. Box 2528
Idaho Falls, ID 83403



} Rado
}
} ---------------------------------------------------------------------
} Radostin Danev
} Laboratory of Ultrastructure Research
} National Institute for Physiological Sciences
} Myodaiji-cho, Okazaki 444-8585, JAPAN
} e-mail: rado-at-nips.ac.jp
} ---------------------------------------------------------------------
} ----- Original Message -----
} } From: Jonathan Barnard {Jonathan.Barnard-at-Angstrom.UU.SE}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, February 02, 2000 7:20 PM
} Subject: TEM:finding the parallel beam
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I got a tip for finding the condenser lens settings required for parallel
} } illumination from Angus Kirkland (Cambridge).
} }
} } Make sure you are in microprobe mode and set up for SAED and the specimen
} } is out of the way. Spread the beam, put in the SA Aperture, go to
} } diffraction and then sweep the SA aperture (SAA) from side to side and
} } minimise the deflection of the diffraction spot by tweaking the
} } illumination control (C2 or second condenser lens). A little bit of
} thought
} } will convince you that anything other than a parallel beam will give you a
} } side to side motion (tilt) when the SAA is swept.
} }
} } I have found it useful to keep a table of C2 condenser lens current
} } settings for each spot size (C1 excitation) in microprobe mode. Setting
} the
} } C2 lens for parallel illumination and adjusting the diffraction focus to
} } get the sharpest spot will then give you near perfect SAD conditions.
} }
} } Anyone contemplating electron holography for example, will have to start
} } from the parallel illumination to get the best spatial coherence for their
} } holograms.
} }
} } ********************************************************
} } Dr Jonathan Barnard
} }
} } Analytical Materials Physics
} } The Angstrom Laboratory, Uppsala University
} } P O Box 534, SE-751 21 Uppsala, Sweden
} } Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
} } http://www.angstrom.uu.se/analytical/home.html
} }
} } ********************************************************
} }
} }
}



From daemon Thu Feb 03 15:59:01 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Thu, 3 Feb 2000 15:11:42 -0500
Subject: Re: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eugenia,

You can avoid your concern with C coating by coating your standards
and unknowns at the same time. You should use the minimum coating
thickness--just enough to prevent beam charging.

However, as stated below by Dr. Lachowski, ammonia volatility would
be a big problem, especially since you will need help from high beam
intensities and/or long counting times to get enough N counts to give
decent precision. The expected intensity measured with a 10kV beam
from a zeolite nominally of analcime composition (NaAlSi2O6.H2O) with
5 wt% N would be about 2.5% that of the intensity from Si3N4. High N
intensity is difficult to achieve even with the best spectrometer and
crystal designs. And to add insult to injury, the addition of the C
coating absorbs even more of the N x-rays from the sample. The mass
absorption coefficient for N K-alpha emission with a C absorber is
huge (greater than 23,000).

Plus, you should check for possible N peak shape differences between
Si3N4 (or other standards) and your ammonium-bearing zeolite.

All in all, it is a very difficult analytical problem.

Good luck!

Carl

At 4:24 PM +0000 2/3/00, Dr Eric Lachowski wrote:
} Eugenia,
} the microprobe is not the best tool for the analysis of
} ammonia in zeolites because ammonia is very volatile in the
} electron beam. If your zeolite is phase pure it would be
} far better to dissolve it and do a classical chemical
} analysis.
}
} regards,
} Eric
} On Thu, 3 Feb 2000 14:54:20 +0100 bengi {bengi-at-unimo.it}
} wrote:
}
} }
} } Dear listers,
} } I have started a research project on ammonium -bearing mineral (zeolites),
} } and now I'm trying to quantify N using electron microprobe analysis. My
} } problems of dealing with N are:
} }
} } a) The thickness of the coating (in the literateur =
} approximately 150 A°).
} } Does anyone have information on the model of coater to making these films?
} } Does anyone have experience with preparation of such
} } samples?
} }
} } b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
} } N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
} } standards for such a sample ?
} }
} } Any other suggestions about quantitative nitrogen analysis would be greatly
} } appreciated !!
} } Thanks for your time.
} }
} } My best regards
} }
} } Eugenia
} }
} }
} } Eugenia Marchi
} } Università degli Studi di Modena
} } Dip. Scienze della Terra
} } P.le S.Eufemia n°.19
} } 41100 Modena (Italy)
} } Tel. +39-059-417289
} } Fax. +39-059-417399
} } e-mail: bengi-at-unimo.it
} }
} }
} }
}
} ----------------------
} Dr Eric Lachowski
} Department of Chemistry
} University of Aberdeen
} Meston Walk
} Old Aberdeen AB24 3UE
} Scotland
} +44 (0)1224 272934 fax 272921
} e.lachowski-at-abdn.ac.uk

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Thu Feb 03 17:53:40 2000



From: Paul.Nolan-at-Alcan.Com
Date: Thu, 3 Feb 2000 16:05:19 -0600
Subject: Technoorg-Linda Ion Beam Thinner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: {Microscopy-at-MSA.Microscopy.Com}


Does anyone out there have experience with the Technoorg-Linda IV3H ion beam
thinner.
We have their instruction manual but it has been a long time since we had a
training session and are having some problem with the set up.
Does anyone have a revised instruction manual or perhaps some tips on the
alignment procedure of the guns. (please please please please).

Thanks
Paul Nolan

P.S..
It is cold in Canada..everyday...all year. hehe




From daemon Thu Feb 03 17:53:43 2000



From: Thomas, Larry :      Larry.Thomas-at-pnl.gov
Date: Thu, 03 Feb 2000 14:10:01 -0800
Subject: RE: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Laurie,

Now I'm really confused. I used to think I understood this somewhat.

1. In the JEOL 2010F FEG (the "all bets are off" case in your item#7),
changing the illumination spread by changing C2 profoundly moves the
"diffraction plane" relative to the objective aperture. [There are actually two
objective apertures at different heights in this microscope, but that's beside
the point]. By diffraction plane, I mean the plane below the objective lens
where the diffraction spots are in sharp focus. In other words (assuming fixed
OL and condenser minilens excitations), in this microscope there is only one C2
value that allows the diffraction spot pattern and OL aperture to be in focus
simultaneously. If you focus on the OL aperture (with the intermediate lens),
the spot pattern can be focused at only this one C2 setting. At any other C2
setting, you can focus the diffraction pattern with the intermediate lens
("diffraction focus") but the OL aperture will then be defocused. Changing the
condenser minilens excitation changes the diffraction plane (relative to the OL
aperture) to a different C2 setting. This is actually quite a useful feature of
the microscope, e.g., for getting very intense focused diffraction patterns, but
a different story.

2. The reason for choosing to focus the diffraction spots rather than
the K-lines is --to put it simply-- that's where the intensity is. In amplitude
contrast imaging (conventional brightfield and darkfield imaging), the intensity
contribution to the image is limited by the OL aperture. It's really important
to have the defining aperture and diffraction spot pattern in focus
simultaneously. In addition, if I aim to measure diffraction spot patterns
there is little incentive to focus the K-lines instead of the spots.

3. Textbooks such as Hirsch et al. often discuss the positional error
in SA diffraction due to Cs. What readers often miss is the consequence of the
fact that the error depends strongly on the diffraction angle. If measurements
are confined to the low-index (low-angle) reflections, the selecting area can be
reduced (to 200 nm or so at 200 kV) without big positional errors. Maybe that's
an example of differences in experimental and theoretical viewpoints.

4. Reducing the condensor aperture size will give less illumination,
but it won't give parallel illumination. It will change the size of the
diffraction spots, but not their focus.

5. I'm not quite sure why the illumination spread has such a strong
effect on the apparent position of the diffraction plane relative to the OL
aperture, but suspect it arises from the action of the strong
condensor-objective in this microscope. I would also question that it has much
to do with coherent/incoherent beams in a FEG or with prefield/postfield Cs
effects.

Larry


Larry Thomas
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto: Larry.Thomas-at-pnl.gov



----------
From: L. D. Marks
Reply To: L-marks-at-nwu.edu
Sent: Thursday, February 3, 2000 8:06 AM
To: Radostin Danev
Cc: MSA listserver
Subject: Re: TEM:finding the parallel beam

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Sorry, but I think we are getting a little confused here.

1) The diffraction plane is (by definition) the objective lens
focal length below the sample - it is the focus of parallel
directions coming from the sample. The focal length (and
parameters such as Cs, Cc) change rapidly with the objective lens
current so an eucentric position makes life simple but is not
necessarily the best condition to use. (For HREM you generaly
want to have the objective lens stronger and reduce Cs.)

2) In general C2 does not change the focal length at all. You
can have some effect from coupling of the magnetic fields, but
all C2 is supposed to do is change the convergence angle.

3) The objective aperature is (approximately) in the back-focal
plane, given that it is finite and height adjustments are only
done coarsely (for a specific value of the objective current
only).

4) The selected area aperature is (approximately) in the same
plane as the object for the same reasons as above. Remember that
there are positional errors in SA diffraction due to Cs (see
Hirsch et al for instance).

5) You should focus Kikuchi-lines, not go for the smallest spot,
since these are real object in the diffraction pattern. What you
will see is then representative of you incident beam, sometimes
a Gaussian range of directions.

6) The simplest way to get more parallel illumination in general is
to reduce the condensor aperture size.

7) All bets are off if you have a FEG. Rather than having incoherent
illumination things like the Cs of the prefield (above the sample)
and postfield (below) add coherently making it much more complicated.
For instance, the illumination angle varies across the field of view.
While this is also there with LaB6, it is a weak effect (except for
certain classes of diffracted beams, e.g. those with a screw-axis).
With
a FEG you can get a "spot" pattern many ways, and most of these will
have
large aberrations (e.g. pincushion).

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++




From daemon Thu Feb 03 17:54:04 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 3 Feb 2000 17:57:35 -0500
Subject: Technoorg-Linda Ion Beam Thinner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul:

South Bay Technology does have quite a bit of experience with the TL IV3
Ion Mill and we are happy to offer our assistance. We market the IV3
system throughout the world for Technoorg-Linda and offer technical support
as well. I will send you the most up to date IV3 manual that we have
produced and will include by separate e-mail the section on the gun
alignment.

I will follow up with you to be sure that you have all of the information
you need.

Best regards-

David
Writing at 3:50:09 PM on 02/03/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
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} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"Paul.Nolan-at-Alcan.Com"-at-sparc5.Microscopy.Com
}
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Does anyone out there have experience with the Technoorg-Linda IV3H ion
beam
thinner.
We have their instruction manual but it has been a long time since we had
a
training session and are having some problem with the set up.
Does anyone have a revised instruction manual or perhaps some tips on the
alignment procedure of the guns. (please please please please).

Thanks
Paul Nolan

P.S..
It is cold in Canada..everyday...all year. hehe
{



From daemon Thu Feb 03 17:54:01 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Thu, 3 Feb 2000 17:08:04 -0600 (CST)
Subject: RE: TEM:finding the parallel beam

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On Thu, 3 Feb 2000, Thomas, Larry wrote:

} Laurie,
}
} Now I'm really confused. I used to think I understood this somewhat.
}
} 1. In the JEOL 2010F FEG (the "all bets are off" case in your item#7),
} changing the illumination spread by changing C2 profoundly moves the
} "diffraction plane" relative to the objective aperture. [There are actually two
} objective apertures at different heights in this microscope, but that's beside
} the point]. By diffraction plane, I mean the plane below the objective lens
} where the diffraction spots are in sharp focus. In other words (assuming fixed
} OL and condenser minilens excitations), in this microscope there is only one C2
} value that allows the diffraction spot pattern and OL aperture to be in focus
} simultaneously. If you focus on the OL aperture (with the intermediate lens),
} the spot pattern can be focused at only this one C2 setting. At any other C2
} setting, you can focus the diffraction pattern with the intermediate lens
} ("diffraction focus") but the OL aperture will then be defocused. Changing the
} condenser minilens excitation changes the diffraction plane (relative to the OL
} aperture) to a different C2 setting. This is actually quite a useful feature of
} the microscope, e.g., for getting very intense focused diffraction patterns, but
} a different story.
}
OK, the bets off case. In a FEG you have a somewhat coherent
range of incident directions. The "diffraction pattern" is therefore
something which is effected by the coherent aberrations of the microscope,
unlike the case with incoherent illumination. Consider the case with
focussed illumination, when the diffraction pattern shows an image of
the condensor aperture. In a FEG As a you can "focus" this (coherent)
image to a small spot by going out of focus in a true sense for the
diffraction pattern. This gives you a spot-like pattern, but you will also
see severe distortions at higher angles. With incoherent illumination you
cannot do this and going out of focus (in the diffraction pattern) will
give in general a blurred image of the condensor aperture - life is
simple.
What you need to do is focus "real diffraction" features, e.g.
Kikuchi lines, then not play with the diffraction focus at all. As you
change C2 the size of the spots will grow or shrink, this is fine.

} 2. The reason for choosing to focus the diffraction spots rather than
} the K-lines is --to put it simply-- that's where the intensity is. In amplitude
} contrast imaging (conventional brightfield and darkfield imaging), the intensity
} contribution to the image is limited by the OL aperture. It's really important
} to have the defining aperture and diffraction spot pattern in focus
} simultaneously. In addition, if I aim to measure diffraction spot patterns
} there is little incentive to focus the K-lines instead of the spots.
}
Yes, you can always get a "spot" pattern with a FEG, and it may
be prettier. However, beware the distortions which make doing
measurements from it very dangerous.

} 3. Textbooks such as Hirsch et al. often discuss the positional error
} in SA diffraction due to Cs. What readers often miss is the consequence of the
} fact that the error depends strongly on the diffraction angle. If measurements
} are confined to the low-index (low-angle) reflections, the selecting area can be
} reduced (to 200 nm or so at 200 kV) without big positional errors. Maybe that's
} an example of differences in experimental and theoretical viewpoints.
}
Hirsch et al is just a good reference to read, about this as well
as many other things!

} 4. Reducing the condensor aperture size will give less illumination,
} but it won't give parallel illumination. It will change the size of the
} diffraction spots, but not their focus.
}
It makes it closer to parallel. Of course, with really parallel
illumination there is no intensity!

} 5. I'm not quite sure why the illumination spread has such a strong
} effect on the apparent position of the diffraction plane relative to the OL
} aperture, but suspect it arises from the action of the strong
} condensor-objective in this microscope. I would also question that it has much
} to do with coherent/incoherent beams in a FEG or with prefield/postfield Cs
} effects.
}
Sorry, I don't think that is correct. I would suggested that
people look at the distortions with a standard sample and see how bad they
can be.

} Larry
}
}
} Larry Thomas
} Pacific Northwest National Laboratory
} MSIN P8-16
} P.O. Box 999
} Richland, WA 99352
} Phone: (509)372-0793 Fax: (509)376-6308
} Email: mailto: Larry.Thomas-at-pnl.gov
}
}
}
} ----------
} From: L. D. Marks
} Reply To: L-marks-at-nwu.edu
} Sent: Thursday, February 3, 2000 8:06 AM
} To: Radostin Danev
} Cc: MSA listserver
} Subject: Re: TEM:finding the parallel beam
}
} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Sorry, but I think we are getting a little confused here.
}
} 1) The diffraction plane is (by definition) the objective lens
} focal length below the sample - it is the focus of parallel
} directions coming from the sample. The focal length (and
} parameters such as Cs, Cc) change rapidly with the objective lens
} current so an eucentric position makes life simple but is not
} necessarily the best condition to use. (For HREM you generaly
} want to have the objective lens stronger and reduce Cs.)
}
} 2) In general C2 does not change the focal length at all. You
} can have some effect from coupling of the magnetic fields, but
} all C2 is supposed to do is change the convergence angle.
}
} 3) The objective aperature is (approximately) in the back-focal
} plane, given that it is finite and height adjustments are only
} done coarsely (for a specific value of the objective current
} only).
}
} 4) The selected area aperature is (approximately) in the same
} plane as the object for the same reasons as above. Remember that
} there are positional errors in SA diffraction due to Cs (see
} Hirsch et al for instance).
}
} 5) You should focus Kikuchi-lines, not go for the smallest spot,
} since these are real object in the diffraction pattern. What you
} will see is then representative of you incident beam, sometimes
} a Gaussian range of directions.
}
} 6) The simplest way to get more parallel illumination in general is
} to reduce the condensor aperture size.
}
} 7) All bets are off if you have a FEG. Rather than having incoherent
} illumination things like the Cs of the prefield (above the sample)
} and postfield (below) add coherently making it much more complicated.
} For instance, the illumination angle varies across the field of view.
} While this is also there with LaB6, it is a weak effect (except for
} certain classes of diffracted beams, e.g. those with a screw-axis).
} With
} a FEG you can get a "spot" pattern many ways, and most of these will
} have
} large aberrations (e.g. pincushion).
}
} ++++++++++++++++++++++++++++++++++++++++++++++++
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} fax: (847) 491-7820
} mailto:l-marks-at-nwu.edu
} http://www.numis.nwu.edu
} ++++++++++++++++++++++++++++++++++++++++++++++++
}
}
}

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:L-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++




From daemon Thu Feb 03 20:37:06 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 4 Feb 2000 12:30:27 +1100
Subject: Re: LSCM Need help with non-fluorescing resin

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Butyl methyl methacrylate is in our hands non fluorescent
totally. You can use it for TEM, although it is not as good as epoxies.
Hope this helps.
Tobias
}
}
} I am working on fluorescent imaging of thin sections cut from samples
} embedded in TEM embedding resin, and am having a significant problem with
} resin fluorescence. Does anyone know of an embedding resin that doesn't
} autofluoresce?
}
} Matt Plantinga
} Amway Corporation
} matt_plantinga-at-amway.com



From daemon Thu Feb 03 20:36:55 2000



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Date: Thu, 03 Feb 2000 19:34:55
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From daemon Thu Feb 03 17:54:05 2000



From: ctschristopher :      ctschristopher-at-samiot.uct.ac.za (by way of Carol
Date: Thu, 3 Feb 2000 17:56:46 -0800
Subject: a question about 2-hydroxyhexane-dial, AKA hydroxyadipaldehyde

Contents Retrieved from Microscopy Listserver Archives
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LIst,

Does anyone know of a current source for this chemical? It's an ingredient
of an embedding medium made up by our group. We have a supply on hand but
have to reserve it if it's no longer commercially available.


} } We are very excited about the possibilities of HACH as a solution for a
} } problem
} } that has troubled us for a couple of years with our PU grafts. We have,
} } unfortunately, one serious problem in that we cannot find a source for HA.
} } We have tried Sigma Aldrich and they informed us that they withdrew
} } 2-Hydroxyhexane-dial from their range in 1990. We have tried a web search
} } and are contacting our local suppliers, so far without success. Do you have
} } any ideas where we can find it? We are very anxious to try HACH on our PU
} } samples and will gratefully appreciate any additional help that you can give
} } us.

} } Thanks again

} } Phil




From daemon Fri Feb 04 21:48:26 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Fri, 04 Feb 2000 09:04:34 +0200
Subject: Silence

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Have I been cut off, or is nobody talking anymore?

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University e-mail: malc-at-rock.ru.ac.za
6140 Grahamstown
SOUTH AFRICA




From daemon Fri Feb 04 21:48:27 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Fri, 4 Feb 2000 08:51:22 +0100 (MET)
Subject: Re: New Gold Enhancement Reagents

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Dear Michael,

I have tried GoldEnhance for preembedding protocol to label intracellular
structures. It is marvelous. Buy it and use it - you will not be
unsatisfied. I have no any interest in Nanoprobes, I just like these dense
gold particles.
Practically all they claim is true:
- light insensitive
- no self-nucleation
- do not react with buffer ions
- is not dissolved by uranil and osmium (I have treat for 1 h)

Sincerely,
Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it


On Thu, 3 Feb 2000, Michael Plociniak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
} Has anyone used gold enhancement reagents from Nanoprobes, Inc.
} (Stonybrook, NY - USA) as an alternative to silver enhancement for
} enlarging 1 nm immunogold probes?
}
} Advertisements state that gold enhancement has lower background and is
} compatible with osmium. Can anyone verify this from personal experience?
}
} In addition to these advantages, I am hoping that milder pH conditions will
} be less damaging to ultrastructure in cultured neurons (using a
} preembedment protocol).
}
} Thank you,
} Michael
}
}



From daemon Fri Feb 04 21:48:31 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 4 Feb 2000 00:13:57 -0800 (PST)
Subject: Re: Help:A comparison of optics

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Dear Steve and interested parties:

The comparison between Zeiss and Olympus '100x' objective lenses is
impossible unless one knows the type of lens be compared.

Catagories used to determine the quality of such a lens include:
aberration correction (spherical and chromatic), correction of flatness of
field, numerical aperture, the presence of an iris diaphram, whether the
system is based on a 160mm tube length or infinity correction, and if the
lens is used for brightfield or phase contrast for instance.

(PLAN APO, PH3, 100x, iris diaphram, 1.32 numerical aperture? 160mm tube
length)

Resolution is still basically related to half the wavelength utilized.
Hey, let's look at the advantages of oblique illumination for contrast
enhancement and a differerent formula for resolution!!

Is your client looking at a system or an indivdual lens? Do they have a
Zeiss or an Olympus system? One does not mixed and match objectives from
different systems merely for the convenience of pricing.

An additional consideration is the type and correction of the condenser
lens. Check the numerical aperture of not only the objective, but the
condenser lens as well.

More questions than answers...Let me know if I can further complicate the
answers!

Cheers!
Ken

------------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N Willamette Blvd.
Portland, OR 97303


On Thu, 3 Feb 2000, Steve Niemela wrote:

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} To: {Microscopy-at-MSA.Microscopy.Com}
} Subject: A comparison of optics
} Date: Thu, 3 Feb 2000 10:57:19 -0600
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}
} A client of ours wonders about a comparison between Zeiss and Olympus
} optics. The Zeiss 100X lense cost $10,000, and the Olympus about $4,000:
} does that mean the Olympus is inferior?  That's what our client's
} colleagues imply. What measure of lense function is relevant? Does Olympus
} (or Zeiss) have a specification, like 'resolves particles to 0.1 micron'
} or something like that?  Is there a measure of distortion? Best
} regards, Steve NiemelaVayTek, Inc.305 West Lowe AvenueFairfield, IA
} 52556email: sniemela-at-vaytek.com Phone: 515.472.2227Fax: 515.472.8131web:
} www.vaytek.comgeneral email: vaytek-at-vaytek.com
}
}
}
}



From daemon Fri Feb 04 21:48:33 2000



From: Janko Otto :      otto-at-quantifoil.com
Date: Fri, 04 Feb 2000 09:47:44 +0100
Subject: Re: JAMP-7810 manual in English and local chemical analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dmitri,

} We are making preparations for experiments on determination of the chemical
} composition of semiconductor oxides, formed locally. The size of the
} modified area should be about 1 micron, and we are not sure yet if it is
} possible to make mentioned chemical analysis with Auger microprobe at all.

I can recommend you some former colleagues of mine performing auger
spectroscopy and many other semiconductor surface analytics.
Please contact Prof. Wolfgang Richter: richter-at-pinet.uni-jena.de.
The URL is: http://www.physik.uni-jena.de/~layer/

Regards,
Kay Pfennighaus


--
_____________________________________________________________

Janko Otto/Kay Pfennighaus email otto-at-quantifoil.com
Quantifoil Micro Tools GmbH Tel +49 (0) 3641 - 206 470
Winzerlaer Strasse 10 Fax +49 (0) 3641 - 206 471
D-07745 Jena Web http://www.quantifoil.com
Germany
_____________________________________________________________



From daemon Fri Feb 04 21:48:35 2000



From: Tony Bruton :      bruton-at-EMU.UNP.AC.ZA
Date: Fri, 04 Feb 2000 11:38:49 +0200
Subject: Re: Silence

Contents Retrieved from Microscopy Listserver Archives
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W'ere all waiting to hear news of MSSA Conference 2000 { :-)

Tony

} } } Dr Malcolm Roberts {malc-at-rock.ru.ac.za} 02/04/00 09:04AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Have I been cut off, or is nobody talking anymore?

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University e-mail: malc-at-rock.ru.ac.za
6140 Grahamstown
SOUTH AFRICA






From daemon Fri Feb 04 21:48:38 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Fri, 4 Feb 2000 10:10:23 +0000
Subject: Re: Technoorg-Linda Ion Beam Thinner

Contents Retrieved from Microscopy Listserver Archives
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Hi Paul,

} Does anyone out there have experience with the Technoorg-Linda IV3H ion beam
} thinner.
} We have their instruction manual but it has been a long time since we had a
} training session and are having some problem with the set up.
} Does anyone have a revised instruction manual or perhaps some tips on the
} alignment procedure of the guns. (please please please please).

We have the IV3 here in Sheffield - I'll try and help you if I can.
You need to be able to actually see the beams (cover your head and
the viewing glass with a black sheet if you cannot darken the room).
Get one gun to fire through the center of the sample holder (tilt it
at right angles to the beam) and to hit the opposite gun where it's
beam would exit. Do the same for the other gun. Now keep this gun
orientation, load the specimen and tilt the holder to the desired milling angle.

Hope this helps,

Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://www.shef.ac.uk/uni/academic/D-H/eee/
*********************************************



From daemon Fri Feb 04 21:48:59 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Fri, 4 Feb 2000 09:17:35 -0500 (EST)
Subject: Is KEVEX 8000 worth installing?

Contents Retrieved from Microscopy Listserver Archives
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A Kevex 8000 (with huge 8 or 10" disk drives), attached to an old Amray
1000, has been donated to us. All components were fully functional when
they were crated up a year ago. (They are sitting in our basement until we
move into our new building this June).

A colleague in Physics (I'm a biologist) wishes to use the system to
quantify elemental composition (beryllium, gallium, etc) in semi-conductor
ceramics. We already have (up and running) an Hitachi HS-510 (SEM) and an
Hitachi H-7100 (STEM).

1) Is this (Kevex 8000) an appropriate instrument for her to do the
analyses?

2) Which would be wiser: Trying to get the Amray installed and running
or getting an adapter to use the Kevex in one of the Hitachi's? (Space is
a consideration, so I'd prefer not to have to install (and keep in running
condition) another scope.)

3) The Kevex is pretty old. Are we wasting money trying to get or keep
running something that is this old? I realize that we may need to replace
the detector, since it has been at room temp for about a year. Is there
anything else we should automatically consider replacing or upgrading as
part of the installation?

4) She also has been sending her samples out to do x-ray analysis of the
material to determine it's crystalline structure. The place she sends it
to has a special x-ray diffraction instrument. Could the x-ray
diffraction on the H-7100 be used to get equal results, or does this other
instrument have capabilities that the TEM doesn't.

Thanks for any advice.

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718








From daemon Fri Feb 04 21:49:36 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 04 Feb 2000 10:20:50 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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Steve,

The cost of an objective depends on a number of issues, not the least of
which are the corrections for chromatic and spherical aberration as well as
the size of the numerical aperture. I'd need more information to really
compare. "Optimizing Light Microscopy" has a detailed discussion of all of
this which might be helpful. Ordering information is available on our
website.

The best test is to try each system with your applications. Since much of
Vaytek's work centers on deconvolution of fluorescence images, fluorescent
beads of varying sizes would be a good test object for you and high
throughput and crisp imaging would probably be two key benchmarks. Since
fluorescence detects objects beyond the resolution limits, the normal
resolution tests are meaningless. By the way, the numerical aperture of
the objective is the major deciding factor on a microscope's ability to
resolve fine detail (as well as providing good edge information).

If you'd like to write or call me directly, I may be able to give you
further guidance.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


Caveat: MME does have a commercial interest in the sale of "Optimizing
Light MIcroscopy:"

At 04:04 PM 2/3/00 -0600, Steve Niemela wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Feb 04 21:49:47 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 04 Feb 2000 09:52:32 -0600
Subject: Re: Is KEVEX 8000 worth installing?

Contents Retrieved from Microscopy Listserver Archives
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If your Kevex has a Quantum thin window detector you should be able to see
about half of a Boron peak, but there is no commercially available detector
(that I know of) that reliably sees all of the beryllium peak. And if you
have a standard window, you will only be able to see sodium and above.

I suppose the detector may still be functional even after storage. I recall
that it is not a trivial thing to switch detectors between scope models.
The adapters can be quite different and can get pricey. But I have not
priced one in over 10 years.

I suppose with rigorous use of standards, you might be able to analyze Be
by difference, but it would be a trick.

I think the Kevex 8000 may be worth installing if you are primarily
interested in x-ray analysis. We used a Kevex Delta for many years and
found it quite adequate. I might be able to talk you through some of the
technical issues.

However, if you are interested in digital imaging or x-ray mapping, the
improvements in the new equipment is fantastic compared to the old systems.
We run an IXRF Systems EDS on a JEOL 840A (having replaced our Kevex) and a
Link ISIS on a Hitachi 2460N. I could hardly imagine giving up their
capabilities and going back to the Delta or an 8000. Disk storage, the
operating environment, digital imaging can hardly be compared between the
old and the new.

FWIW,
Warren

At 09:17 AM 2/4/2000 -0500, Donald L. Lovett wrote:
} A Kevex 8000 (with huge 8 or 10" disk drives), attached to an old Amray
} 1000, has been donated to us. All components were fully functional when
} they were crated up a year ago. (They are sitting in our basement until we
} move into our new building this June).
}
} A colleague in Physics (I'm a biologist) wishes to use the system to
} quantify elemental composition (beryllium, gallium, etc) in semi-conductor
} ceramics. We already have (up and running) an Hitachi HS-510 (SEM) and an
} Hitachi H-7100 (STEM).
}
} 1) Is this (Kevex 8000) an appropriate instrument for her to do the
} analyses?
}
} 2) Which would be wiser: Trying to get the Amray installed and running
} or getting an adapter to use the Kevex in one of the Hitachi's? (Space is
} a consideration, so I'd prefer not to have to install (and keep in running
} condition) another scope.)
}
} 3) The Kevex is pretty old. Are we wasting money trying to get or keep
} running something that is this old? I realize that we may need to replace
} the detector, since it has been at room temp for about a year. Is there
} anything else we should automatically consider replacing or upgrading as
} part of the installation?
}
} 4) She also has been sending her samples out to do x-ray analysis of the
} material to determine it's crystalline structure. The place she sends it
} to has a special x-ray diffraction instrument. Could the x-ray
} diffraction on the H-7100 be used to get equal results, or does this other
} instrument have capabilities that the TEM doesn't.
}
} Thanks for any advice.
}
} Don



From daemon Fri Feb 04 21:49:48 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Fri, 04 Feb 2000 10:55:56 -0500
Subject: Re: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
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Carl et al.,

I responded to Eugenia's query on another listserver (MAS microprobe listserver),
but since it is also discussed here, I will add a couple things.

I did some work on ammonium-bearing minerals (with ~ 3 - 5 wt%N), using BN and later
synthetic buddingtonite (ammonium feldspar) as the N standard (see Microbeam
Analysis 1994, New Orleans meeting). I avoided using any epoxy for the sample prep
as a precaution against N artifacts from the mounting medium. The synthetic
buddingtonite can be made ala Voncken et al., 1988 (Phys. Chem of Minerals, v. 15,
p. 323).

We did "normal" carbon coating, using brass color as a guide to obtain 150-200A
coating (standards and unknowns). I used a synthetic crystal, 2d 60A, and ran at
10kV, 25 nA probe current. I agree that a light C coat, done at same time/conditions
as the standards is important. I don't recall a substantial problem with mobility
at thnese operating conditions, although the N intensity yields were low and
detection limits were on the order of 0.5wt% N. The minerals are feldpar and
perhaps the ammonium ion stayed put better than it woud in zeolites. I agree
though, it is a difficult analytical problem. But that makes it interesting.

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610




Carl Henderson wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Eugenia,
}
} You can avoid your concern with C coating by coating your standards
} and unknowns at the same time. You should use the minimum coating
} thickness--just enough to prevent beam charging.
}
} However, as stated below by Dr. Lachowski, ammonia volatility would
} be a big problem, especially since you will need help from high beam
} intensities and/or long counting times to get enough N counts to give
} decent precision. The expected intensity measured with a 10kV beam
} from a zeolite nominally of analcime composition (NaAlSi2O6.H2O) with
} 5 wt% N would be about 2.5% that of the intensity from Si3N4. High N
} intensity is difficult to achieve even with the best spectrometer and
} crystal designs. And to add insult to injury, the addition of the C
} coating absorbs even more of the N x-rays from the sample. The mass
} absorption coefficient for N K-alpha emission with a C absorber is
} huge (greater than 23,000).
}
} Plus, you should check for possible N peak shape differences between
} Si3N4 (or other standards) and your ammonium-bearing zeolite.
}
} All in all, it is a very difficult analytical problem.
}
} Good luck!
}
} Carl
}
} At 4:24 PM +0000 2/3/00, Dr Eric Lachowski wrote:
} } Eugenia,
} } the microprobe is not the best tool for the analysis of
} } ammonia in zeolites because ammonia is very volatile in the
} } electron beam. If your zeolite is phase pure it would be
} } far better to dissolve it and do a classical chemical
} } analysis.
} }
} } regards,
} } Eric
} } On Thu, 3 Feb 2000 14:54:20 +0100 bengi {bengi-at-unimo.it}
} } wrote:
} }
} } }
} } } Dear listers,
} } } I have started a research project on ammonium -bearing mineral (zeolites),
} } } and now I'm trying to quantify N using electron microprobe analysis. My
} } } problems of dealing with N are:
} } }
} } } a) The thickness of the coating (in the literateur =
} } approximately 150 A°).
} } } Does anyone have information on the model of coater to making these films?
} } } Does anyone have experience with preparation of such
} } } samples?
} } }
} } } b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
} } } N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
} } } standards for such a sample ?
} } }
} } } Any other suggestions about quantitative nitrogen analysis would be greatly
} } } appreciated !!
} } } Thanks for your time.
} } }
} } } My best regards
} } }
} } } Eugenia
} } }
} } }
} } } Eugenia Marchi
} } } Università degli Studi di Modena
} } } Dip. Scienze della Terra
} } } P.le S.Eufemia n°.19
} } } 41100 Modena (Italy)
} } } Tel. +39-059-417289
} } } Fax. +39-059-417399
} } } e-mail: bengi-at-unimo.it
} } }
} } }
} } }
} }
} } ----------------------
} } Dr Eric Lachowski
} } Department of Chemistry
} } University of Aberdeen
} } Meston Walk
} } Old Aberdeen AB24 3UE
} } Scotland
} } +44 (0)1224 272934 fax 272921
} } e.lachowski-at-abdn.ac.uk
}
} ======================================
} Carl Henderson
} Electron Microbeam Analysis Laboratory
} University of Michigan
} 2501 C.C. Little Bldg.
} Ann Arbor, MI 48109-1063 USA
} (734) 936-1550 FAX (734) 763-4690
} ======================================



From daemon Fri Feb 04 21:49:50 2000



From: Augusto_A_Morrone-at-notes.seagate.com
Date: Fri, 4 Feb 2000 09:58:13 -0600
Subject: JEOL 2010 question/C2, diffraction and OA

Contents Retrieved from Microscopy Listserver Archives
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Since the discussion on CM12 alignment shifted to several other problems, and
eventually got some JEOL 2010 users involved (Larry Thomas), I thought of
throwing in this alignment question regarding the OA in a 2010 with a LaB6 gun:

To keep the camera length at a consistent value when I am not using an internal
standard, I record SA diffraction patterns with C2 fully defocused cw (this is
by conventional jargon, although actually using the Brightness knob I am
changing C3). This is supposed to make the illumination as parallel as possible
and is easily reproducible. Then I focus the direct beam with the intermediate
lens (diffraction focus). CBD and NBD are another problem.

The problem is to align the Objective Aperture under this condition. If the OA
is centered around the direct beam in diffraction, when switching to image mode
and increasing the illumination (Brightness) for imaging conditions, the
aperture will be apparently out of center. This gives the impression that the
voltage center changes between a fully defocused C2 and a moderately focused
(still cw from crossover, converging the illumination on the sample) condenser.
That shift is obvious in DIFF mode, the direct beam can be seen to shift as C2
is turned ccw from the fully cw position.

As was noted in the on-going discussion, the crossover planes move along the
optic axis as the strength of C2 changes. The helicoidal path of the beam is
straight down the optic axis of the lenses only piecewise, and at the level of
the OA plane it stays centered only for a limited range of C2 settings--or
convergence angles. In my case, the convergence changes considerably when I
record images digitally in a 1kx1k CCD camera, which screams for brightness.

My two ways around this are to (1) recenter the OA in image mode, or (2) in DIFF
mode, but keeping C2 at the setting I will use in MAG mode. Both methods are a
pain, mostly when doing BF/DF work, and probably incorrect.

Is there an alignment procedure for the 2010 that will prevent this " OA shift "
from DIFF to MAG modes?

Augusto Morrone
Seagate Technology
NRW-115
7801 Computer Ave S.
(612) 844-5838
Fax: (612) 844-7301




From daemon Fri Feb 04 21:49:54 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 04 Feb 2000 08:42:59 -0800
Subject: Re: Need repairman for Hitachi SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Paul,
Why don't you use the Hitachi service from NSC? They are the best and the
cheapest.
At 12:12 PM 2/3/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Feb 04 21:49:59 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Fri, 4 Feb 2000 10:12:49 -0800 (PST)
Subject: Aqueous mounting medium

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Can anyone recommend an aqueous mounting medium that polymerises (or
whatever) to form a permanent hard mount, and that does not
autofluoresce? Thanks.

Lesley Weston.





From daemon Fri Feb 04 21:50:02 2000



From: Douglas Keene :      DRK-at-SHCC.ORG
Date: Fri, 04 Feb 2000 10:36:37 -0800 (Pacific Standard Time)
Subject: New Gold Enhancement Reagents

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Michael,

We had been using silver enhancement kits from Amersham and
Nanoprobe. Of these, we found the Nanoprobe kit to be very
difficult to work with given it's high viscosity. We also
use a pre-embed protocol, and found that both silver
enhancement kits caused collagen fibrils to denature
(unravel). However, during our protocol the tissue is
incubated for fairly long duration in the enhancement
medium (15 minutes on ice, then warmed to 25 C in a water
bath for an additional 5 minutes, all in the dark).

Our more recent experience is with the Nanoprobe gold
enhancement kit. It has HUGE advantages over the silver
system.

First, you can Osmicate the tissue. Second, the viscosity
of the components is comparatively very low, easing the use
of the product. It is not so sensitive to light, and it
does not cause denaturation of collagen fibrils. We do see
some variability in particulate size, but this is probably
a problem associated with diffusion of the media through
the tissue. We have found no disadvantages of the Gold
enhancement v.s. Silver and now use the Gold method
exclusively.

I hope this helps,

Doug

On Thu, 03 Feb 2000 12:44:23 -0500 Michael Plociniak
{plocinia-at-aecom.yu.edu} wrote:

}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
} Has anyone used gold enhancement reagents from Nanoprobes,
} Inc. (Stonybrook, NY - USA) as an alternative to silver
} enhancement for enlarging 1 nm immunogold probes?
}
} Advertisements state that gold enhancement has lower
} background and is compatible with osmium. Can anyone
} verify this from personal experience?
}
} In addition to these advantages, I am hoping that milder pH
} conditions will be less damaging to ultrastructure in
} cultured neurons (using a preembedment protocol).
}
} Thank you,
} Michael

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org






From daemon Fri Feb 04 21:50:14 2000



From: Dan Freidus :      freidus-at-wwnet.com
Date: Fri, 4 Feb 2000 14:38:50 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
} From: Ken Tiekotter {tiekotte-at-up.edu}
..One does not mixed and match objectives from different systems merely for
the convenience of pricing....
----------------------------------------------------------------------------
---------------------------------------------------------------------------

Of course, I've heard this sort of statement many times before. But has
anyone ever done any comparisons of mixed systems to see if there are
certain brands that are particularly incompatible or particularly
compatible?

I ask this both as a general question and because I use a Wild M7 for which
I need a pair of high eyepoint oculars. I don't need the adjusting collar
built into current models and haven't been able to find an older pair used.
But before I buy a Nikon/Olympus/ etc. pair I'd love to know how different
the complete system will be from what I've got now.

I know many people who have used third party photo eyepieces, in just the
situation where you'd think quality would be of the utmost concern. Dealers
are often not the best source of info since they tend to represent one brand
and, even when they are completely honest, rarely have experienc ewith a
wide range of brands (especially mixed up.

Dan Freidus
freidus-at-wwnet.com

P.S. Of course, if anyone has a pair of Wild 10x high eyepoint oculars for
sale, that would also solve my problem (I'd also consider other
magnifications). (I'm also looking for accessory objective lenses and
various other accessories. Please contact me off-list if you've got anything
for sale or trade.)






From daemon Fri Feb 04 21:51:06 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 04 Feb 2000 15:02:28 -0600
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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At 09:20 AM 2/4/00 , you wrote:
} } A client of ours wonders about a comparison between Zeiss and Olympus
} } optics. The Zeiss 100X lense cost $10,000, and the Olympus about $4,000:
} } does that mean the Olympus is inferior?  That's what our client's
} } colleagues imply. What measure of lense function is relevant? Does Olympus
} } (or Zeiss) have a specification, like 'resolves particles to 0.1 micron'
} } or something like that?  Is there a measure of distortion? Best
} } regards, Steve NiemelaVayTek, Inc.305 West Lowe AvenueFairfield, IA
} } 52556email: sniemela-at-vaytek.com Phone: 515.472.2227Fax: 515.472.8131web:
} } www.vaytek.comgeneral email: vaytek-at-vaytek.com
} }

The NA is very important in achieving high resolution. The other factor is
the NA of the condenser. An Abbe condenser is easily out gunned by
the aplanatic condenser (NA=1.4). Olympus makes two PlanAPO 100X oil
objectives. One has an NA of 1.3 while the other is 1.4. The 1.4 lens
costs today new about $6000. The lower NA lens is about $4500. So this
is probably the one you are comparing. I had a Zeiss PlanAPO and recall
that it had an NA of 1.3. It was terrible. I replaced it with the Olympus 1.4
and got major improvements. Of the Zeiss objectives, their 63X PlanAPO
is probably the best one they have made. The rest are so so. Some are
easily beaten by Olympus flourites. Zeiss is more expensive because it
is Zeiss, not necessarily because it is better. The cost of manufacturing
in Germany is very high compared to Japan and other places that
Olympus uses. The emerging problem I see is that Olympus is moving much
of its scope production to China. I have purchased all of the Olympus
items I need and that were made in Japan before being stuck with Chinese
Olympus. It may reach a point where Zeiss is a better product despite the
higher cost.

BTW, the 1.4NA lens at $6000 reflects the recent 10% across the board
price increase by Olympus.

gary g.



From daemon Fri Feb 04 21:50:38 2000



From: Sinkler, Wharton :      wharton.sinkler-at-anlw.anl.gov
Date: Fri, 04 Feb 2000 14:14:10 -0700
Subject: Re: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
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Listers,

There are important differences between older and more recent TEMs, which go
to the heart of this discussion.

On older dedicated HREM's (LaB6) imaging is done with the beam at or close
to crossover on the specimen, and beam convergence is determined by the user
using the C2 aperture. On newer TEM's such as the JEOL 2010 (FEG or not),
you need to set C2 so crossover is pretty far from the specimen plane in
order to get decent coherence for imaging. Whenever this is the case, the
C2 aperture isn't incoherently filled, and some of the older theory won't
apply (regardless of whether the machine is or isn't a FEG).

For example, the standard method of determining convergence in an image is
by switching to diffraction under the same conditions used to image, and
measuring the disc diameter. (see e.g. O'Keefe and Sanders, Acta Cryst A31,
p.307). This assumes the beam being near or at crossover on the specimen
aperture. It won't work if the beam is far from crossover on the specimen,
because now the incident angle depends on position on the specimen. The
disc diameters in diffraction only indicate what the total range of incident
angles is over the entire illuminated area. But the area visible in the
HREM image is much smaller than this, and over this latter area the range of
illuminating angles is smaller. This is just another way of saying that the
C2 aperture isn't incoherently filled. If it were, would be impossible to
form a shadow image of the C2 aperture at the specimen plane, as one does by
spreading the beam.

Think of the illumination on the specimen as a convolution of the source
image with the circular C2 aperture: When the beam is at crossover, the
aperture part is approximating a delta function - we see the source in the
image. When the beam is spread very far, the illumination is a nice shadow
image of the C2 aperture, with a little blurriness at the edge because of
convolution with a source which is not quite point-like.

In the latter case, the angular spread locally is given by the source size
(demagnification) in the diffraction pattern. This can be imaged by
switching to diffraction and focusing with the intermediate lens to obtain
sharp source images.

This doesn't depend on whether the machine is FEG or not, though the source
will be very much smaller if it is. I have posted an example in which a
tungsten filament is imaged sharply by defocusing with the intermediate lens
with the illumination just slightly defocused. This should be a square
pattern, so note that the optics introduce significant distortions in this
extreme case.

http://www.numis.nwu.edu/internet/Staff/wharton/sources.jpg


++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler
Argonne National Laboratory West
P. O. Box 2528
Idaho Falls, ID 83403

}
}
} On Thu, 3 Feb 2000, Thomas, Larry wrote:
}
} } Laurie,
} }
} } Now I'm really confused. I used to think I understood this somewhat.
} }
} } 1. In the JEOL 2010F FEG (the "all bets are off" case in your item#7),
} } changing the illumination spread by changing C2 profoundly moves the
} } "diffraction plane" relative to the objective aperture. [There are actually
} } two
} } objective apertures at different heights in this microscope, but that's
} } beside
} } the point]. By diffraction plane, I mean the plane below the objective lens
} } where the diffraction spots are in sharp focus. In other words (assuming
} } fixed
} } OL and condenser minilens excitations), in this microscope there is only one
} } C2
} } value that allows the diffraction spot pattern and OL aperture to be in focus
} } simultaneously. If you focus on the OL aperture (with the intermediate
} } lens),
} } the spot pattern can be focused at only this one C2 setting. At any other C2
} } setting, you can focus the diffraction pattern with the intermediate lens
} } ("diffraction focus") but the OL aperture will then be defocused. Changing
} } the
} } condenser minilens excitation changes the diffraction plane (relative to the
} } OL
} } aperture) to a different C2 setting. This is actually quite a useful feature
} } of
} } the microscope, e.g., for getting very intense focused diffraction patterns,
} } but
} } a different story.
} }
} OK, the bets off case. In a FEG you have a somewhat coherent
} range of incident directions. The "diffraction pattern" is therefore
} something which is effected by the coherent aberrations of the microscope,
} unlike the case with incoherent illumination. Consider the case with
} focussed illumination, when the diffraction pattern shows an image of
} the condensor aperture. In a FEG As a you can "focus" this (coherent)
} image to a small spot by going out of focus in a true sense for the
} diffraction pattern. This gives you a spot-like pattern, but you will also
} see severe distortions at higher angles. With incoherent illumination you
} cannot do this and going out of focus (in the diffraction pattern) will
} give in general a blurred image of the condensor aperture - life is
} simple.
} What you need to do is focus "real diffraction" features, e.g.
} Kikuchi lines, then not play with the diffraction focus at all. As you
} change C2 the size of the spots will grow or shrink, this is fine.
}
} } 2. The reason for choosing to focus the diffraction spots rather than
} } the K-lines is --to put it simply-- that's where the intensity is. In
} } amplitude
} } contrast imaging (conventional brightfield and darkfield imaging), the
} } intensity
} } contribution to the image is limited by the OL aperture. It's really
} } important
} } to have the defining aperture and diffraction spot pattern in focus
} } simultaneously. In addition, if I aim to measure diffraction spot patterns
} } there is little incentive to focus the K-lines instead of the spots.
} }
} Yes, you can always get a "spot" pattern with a FEG, and it may
} be prettier. However, beware the distortions which make doing
} measurements from it very dangerous.
}
} } 3. Textbooks such as Hirsch et al. often discuss the positional error
} } in SA diffraction due to Cs. What readers often miss is the consequence of
} } the
} } fact that the error depends strongly on the diffraction angle. If
} } measurements
} } are confined to the low-index (low-angle) reflections, the selecting area can
} } be
} } reduced (to 200 nm or so at 200 kV) without big positional errors. Maybe
} } that's
} } an example of differences in experimental and theoretical viewpoints.
} }
} Hirsch et al is just a good reference to read, about this as well
} as many other things!
}
} } 4. Reducing the condensor aperture size will give less illumination,
} } but it won't give parallel illumination. It will change the size of the
} } diffraction spots, but not their focus.
} }
} It makes it closer to parallel. Of course, with really parallel
} illumination there is no intensity!
}
} } 5. I'm not quite sure why the illumination spread has such a strong
} } effect on the apparent position of the diffraction plane relative to the OL
} } aperture, but suspect it arises from the action of the strong
} } condensor-objective in this microscope. I would also question that it has
} } much
} } to do with coherent/incoherent beams in a FEG or with prefield/postfield Cs
} } effects.
} }
} Sorry, I don't think that is correct. I would suggested that
} people look at the distortions with a standard sample and see how bad they
} can be.
}
} } Larry
} }
} }
} } Larry Thomas
} } Pacific Northwest National Laboratory
} } MSIN P8-16
} } P.O. Box 999
} } Richland, WA 99352
} } Phone: (509)372-0793 Fax: (509)376-6308
} } Email: mailto: Larry.Thomas-at-pnl.gov
} }
} }
} }
} } ----------
} } From: L. D. Marks
} } Reply To: L-marks-at-nwu.edu
} } Sent: Thursday, February 3, 2000 8:06 AM
} } To: Radostin Danev
} } Cc: MSA listserver
} } Subject: Re: TEM:finding the parallel beam
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Sorry, but I think we are getting a little confused here.
} }
} } 1) The diffraction plane is (by definition) the objective lens
} } focal length below the sample - it is the focus of parallel
} } directions coming from the sample. The focal length (and
} } parameters such as Cs, Cc) change rapidly with the objective lens
} } current so an eucentric position makes life simple but is not
} } necessarily the best condition to use. (For HREM you generaly
} } want to have the objective lens stronger and reduce Cs.)
} }
} } 2) In general C2 does not change the focal length at all. You
} } can have some effect from coupling of the magnetic fields, but
} } all C2 is supposed to do is change the convergence angle.
} }
} } 3) The objective aperature is (approximately) in the back-focal
} } plane, given that it is finite and height adjustments are only
} } done coarsely (for a specific value of the objective current
} } only).
} }
} } 4) The selected area aperature is (approximately) in the same
} } plane as the object for the same reasons as above. Remember that
} } there are positional errors in SA diffraction due to Cs (see
} } Hirsch et al for instance).
} }
} } 5) You should focus Kikuchi-lines, not go for the smallest spot,
} } since these are real object in the diffraction pattern. What you
} } will see is then representative of you incident beam, sometimes
} } a Gaussian range of directions.
} }
} } 6) The simplest way to get more parallel illumination in general is
} } to reduce the condensor aperture size.
} }
} } 7) All bets are off if you have a FEG. Rather than having incoherent
} } illumination things like the Cs of the prefield (above the sample)
} } and postfield (below) add coherently making it much more complicated.
} } For instance, the illumination angle varies across the field of view.
} } While this is also there with LaB6, it is a weak effect (except for
} } certain classes of diffracted beams, e.g. those with a screw-axis).
} } With
} } a FEG you can get a "spot" pattern many ways, and most of these will
} } have
} } large aberrations (e.g. pincushion).
} }
} } ++++++++++++++++++++++++++++++++++++++++++++++++
} } Laurence Marks
} } Department of Materials Science and Engineering
} } Northwestern University
} } fax: (847) 491-7820
} } mailto:l-marks-at-nwu.edu
} } http://www.numis.nwu.edu
} } ++++++++++++++++++++++++++++++++++++++++++++++++
} }
} }
} }
}
} ++++++++++++++++++++++++++++++++++++++++++++++++
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} fax: (847) 491-7820
} mailto:L-marks-at-nwu.edu
} http://www.numis.nwu.edu
} ++++++++++++++++++++++++++++++++++++++++++++++++
}
}



From daemon Fri Feb 04 21:51:05 2000



From: Jaci Lett :      jmlett-at-cid.wustl.edu
Date: Fri, 4 Feb 2000 16:01:45 -0600
Subject: TEM: B-glucuronidase localization

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We are looking to localize B-glucuronidase in the mouse cochlea. We'd like
to stain for the enzyme and still be able to decalcify the cochleas and
process them into Epon-Araldite for LM and TEM without losing the reaction
product. Does anyone have any suggestions for staining or localization
protocols for this enzyme? I'm not certain whether the tissue will be taken
all the way to TEM, but I do know we'll be looking at LM (1 to 4 microns).
I found a method by Hayashi and Hayashi ("Electron Microscopic Cytochemistry
and Immunocytochemistry in Biomedicine", CRC Press, 1993) using hexazonium
pararosanilin (red in LM and electron dense for EM) on 40 micron non-frozen
sections (and then processed for EM) that sounds appropriate, but I'm
concerned that it may not work en bloc (the cochlea is a twisty, windy
beasty).

I posted this request a few months ago and received no assistance (just
individuals also interested in similar protocols and a company promoting
their antibody).

Thanks so much for any direction I can get,

Jaclynn M. Lett, Research Assistant jmlett-at-cid.wustl.edu

Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO 63110

voice: 314-977-0257 fax: 314-977-0030



From daemon Fri Feb 04 21:51:15 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Sat, 05 Feb 2000 00:45:21 +0100
Subject: TEM: Jeol 2010 question

Contents Retrieved from Microscopy Listserver Archives
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So the problem as I have understood it is:

===} changing C3 (brightness) leads to a shift of the bright field (BF)
beam in the back focal plane (BFP) causing the objective aperture to appear
off centre after converging the beam?

If you have aligned the gun properly then I suspect that the (shift) wobble
alignment has not been done properly.
When this button is pressed the microscope goes over to diffraction and you
should see a pair of discs or spots (which represent the two extremes in
tilt when the beam is shifted to its own extremes). The beam tilts are
adjusted to bring the two discs/spots into coincidence. This is done for
both the X and Y shift coils separately.
The X wobble button shifts the beam back and forth (X shift coils), but in
the back focal plane the spot or disc should remain stationary. This is
what you are trying to achieve with this alignment procedure. Afterwards
this should give you a beam that expands about the same point in the back
focal plane. You should find that the objective aperture remains centred in
both diff and image modes after doing this alignment.

As for the crossovers, yes you are right, they do move up and down when
changing the brightness. The back focal plane remains stationary (by
definition as L.Marks said). Anything other than a parallel beam should
give you a disc in the BFP (which represents the angular distribution of
rays incident on the objective lens).

What did you mean by a voltage centre? My interpretation of this is that
the source high tension is wobbled and any shift in the (previously
focussed) image is minimised by tuning the beam tilts. This alignment would
be used for energy filtered TEM (EFTEM) when doing elemental mapping on a
Gatan Imaging Filter (GIF) for example.



********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Fri Feb 04 21:51:14 2000



From: Stephen A. Shaffer :      sshaffer-at-microdataware.com
Date: Fri, 04 Feb 2000 15:47:58 -0800
Subject: LM Meeting Announcement, San Francisco Microscopical Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The San Francisco Microscopical Society Announces its next regular
meeting:

Wednesday, February 9, 2000

Randall Museum
199 Museum Way
San Francisco, CA 94114-1429
(415) 554-9600

7:00 pm

Topic: Introduction to Phase Contrast Microscopy
by Robert D. Griffin, President, SFMS
And: Annual Business Meeting and Election of Officers

Further information, including an important Letter to the Members from
President Griffin, is available at http://www.microdataware.com/sfms .

The San Francisco Microscopical Society (SFMS) is a small, informal
group of light microscopists who meet on a monthly basis to discuss
topics of common interest. All are invited and welcome, regardless of
knowledge level or professional field.



From daemon Fri Feb 04 21:51:19 2000



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Sat, 5 Feb 2000 14:39:24 +1100
Subject: 5th Live-cell Course at UBC: June 19-29

Contents Retrieved from Microscopy Listserver Archives
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Dear Steve,
Because of the nomenclature and the way manufacturers determine their
specifications, it is really difficult to compare optics on paper. When I
find myself in these types of discussions, I find it more useful to compare
optics of similar list price rather than specifications because generally
speaking, in our industry, the more things cost, the more care is taken in
their manufacture. Olympus has a new 100X objective with an NA of 1.65 that
sells for $10k. That would be the optic I would compare to the $10k Zeiss.

Shane Collins
Scientific Instruments

-----Original Message-----
} From: Steve Niemela [mailto:sniemela-at-vaytek.com]
Sent: Thursday, February 03, 2000 2:05 PM
To: microscopy-at-sparc5.microscopy.com


To: {Microscopy-at-MSA.Microscopy.Com}


SECOND ANNOUNCEMENT: UBC LIVE-CELL COURSE: Please register by March!!

Hello all,

The faculty for the 2000 UBC 3D Live-cell Microscopy Course is now set and
we welcome several new faces, including Stephan Hell, Andreas Kriete and
Steve Potter. The whole list is below.

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Ted InouŽ Universal Imaging, PA
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Jason Swedlow University of Aberdeen
o Michael Weis Agriculture Canada


Basic info is below but you can get the entire brochure, including links to
faculty home pages, at

http://www.cs.ubc.ca/spider/ladic/course/brochure.htm

Cheers,

Jim P.

Fifth Annual INTERNATIONAL 10-Day Short Course on

3D Microscopy of Living Cells
June 19 - 29, 2000



Fourth, Post-course Workshop on

3D Image Processing,
July 1 - 3, 2000



Organized by Prof. James Pawley,
(University of Wisconsin-Madison)
(SEE SABBATICAL ADDRESS AT END OF MESSAGE)


in association with Dr. Elaine Humphrey
UBC BioSciences Microscopy Facility:
University of British Columbia
Vancouver, BC, Canada



DATES

Applications must be received by March 1, 2000
Deposit due April 15, 2000
Registration 5:00 - 7:00 PM Sunday, June 18, 2000
First Lecture 7:30 PM Sunday, June 18, 2000
Live-cell Course ends, noon Thursday, June 29, 2000


APPLICATIONS DUE BY MARCH 1, 2000


More information at :
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or

REGARDING COURSE ORGANIZATION:


Prof. James B. Pawley, (on Sabbatical)
Room LG 10, Madsen Building, F-09,
University of Sydney, NSW, 2006
Australia

Ph. 61-2-9351-7548/2351
FAX 61-2-9351-7682
JBPAWLEY-at-FACSTAFF.WISC.EDU

REGARDING APPLICATIONS

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4




THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary improvement
in our ability to view living cells. To help convert this promise to
reality for a wider selection of biological scientists, an intensive
eleven-day residential course concentrating on all aspects of 3D Microscopy
of Living Cells will be held at the University of British Columbia, in June
of 2000. The course includes 4 days on 2D techniques, 6 days of 3D
techniques and a summary presentation. It covers basic microscopy to the
highest level confocal microscopy. (A half-day Pre-course is offered for
any who may need to brush up on basic optics!).

Topics include:
o Quantitative 2D light microscopy
o How to keep your cells alive
o 3D imaging in confocal microscopy
o Widefield/deconvolution techniques
o Two-photon excitation microscopy
o Fluorescent and backscattered light signals
o Dye design, characteristics and use
o Pixelation: The Nyquist Criterion
o Lasers and laser tweezers
o Objectives and aberrations
o Scanning-systems: AODs and mirrors
o Optimal pinhole size/photon efficiency
o Detectors: operation and performance
o Video-rate confocal imaging
o Measuring ion concentrations
o Display and measurement of 3D data

Morning lecture/demonstrations lead to hands-on laboratory exercises each
afternoon that will utilize most of the commercial instruments currently
available for 3D microscopic imaging. Students will work in groups of 3 or
4 throughout the discussion and laboratory sessions, and may complete a
live-cell 3D study on their own specimens. In the first four years, over
100 students from 22 countries have attended. Last year, 11 separate, 3D
microscopical workstations were available for student use under the
supervision of a 17-member international faculty. We expect to have even
more workstations in 2000. Including manufacturers representatives, the
teacher/ student ratio will be almost 2:1.

INTERNATIONAL FACULTY

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Ted InouŽ Universal Imaging, PA
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Jason Swedlow University of Aberdeen
o Michael Weis Agriculture Canada

TUITION

Course tuition is $2,150 US and includes lunches. On receipt of 50%
deposit, students will receive preliminary group assignments and the
textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The
tuition fee includes the textbook, a binder of handouts, and tickets for
the Opening Reception, the Manufacturer's Reception and the Beach Party.
Accommodations and meals other than lunch are not included in the tuition
fee. The Pre-course is $100 US.

APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrollment is limited to about 24 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts on
request to read before the course begins and are encouraged to take the
Pre-course on the afternoon of June 18.


Application forms, and other course information from this and past years,
can be downloaded from the WWW site at:

http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or obtained from:

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4
Phone: 1-604.822-3354
FAX: 1-604.265-5315
Email: ech-at-unixg.ubc.ca.


Application deadlines:

Application forms are due by March 1, 2000!
Deadlines are early to facilitate setting up groups. Successful applicants
will be notified by April 1, and a deposit of 50% must be received by April
15, 2000 to reserve your position. In general, deposit refunds are only be
possible if your position can be filled from the waiting list. The
remainder of the fees is due at registration.

DATES

Applications must be received by March 1, 2000
Deposit due April 15 2000
Registration 5:00 - 7:00 PM Sunday, June 18, 2000
First Lecture 7:30 PM Sunday, June 18, 2000
Live-cell Course ends, noon Thursday, June 29, 2000


TEACHING PHILOSOPHY

As befits teaching in an area at the boundary of "what is now known,"
lecturers have been chosen based on their expertise as scientists working
in the field rather than because they all agree. They are encouraged more
to be provocative than to be prosaic. Students should expect discussion in
areas where differences of opinion exist.

Prior to the course, students will be organized into groups and encouraged
to communicate by email/phone, about the "Living-cell" group projects that
they will pursue during the course and that will be presented to the class
on the last day of the course. It has been found that group interactions
make best use of students' prior experience and can be very effective in
teaching the practical skills covered in a hands-on course of this type.

ARRANGEMENTS FOR LIVE SAMPLES

Students must contact Dr. Elaine Humphrey to make necessary arrangements
for the transport and maintenance of cell lines etc. needed for their
projects. Organisms linked in any way with human disease are not permitted
because of safety considerations. Transport and customs arrangements for
living specimens are entirely the responsibility of the student.
Students also attending the 3D Image Processing Course, may be able to
analyze, process and display some of the 3D data collected from their
specimens


********************************************************************************

Fourth Annual

3D MAGE PROCESSING WORKSHOP

The workshop will cover 3D image processing for measurement and display.
Enrollment is limited to those attending the 3D Microscopy course.
Tuition : $850 US (lunches and snacks incl.)

WHO SHOULD ATTEND?

The course is designed for biologists working with multidimensional and
possibly multicolor microscopical data sets. Getting the data is only half
of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to
store let alone analyze or display. This course is to help students
understand the hardware and software aspects of this problem and give them
the techniques they need to make the best use of their data.

The course is designed for biologists who need to make measurements on 3D
microscopical data sets and then display the results in an effective
manner. The course will be taught on SGI, Macintosh and IBM-compatible
computers. A wide variety of software designed for the 3D microscopy market
will be described, demonstrated and available for use.

Workshop Organizers

o Andreas Kriete Giessen University, DE
o Felix Margadant University of Sydney, Au

Faculty

o Ping Chin Cheng State U. of New York, Buffalo
o Chris MacLean Vaytek Inc., IA

PLAN OF INSTRUCTION
Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations
followed immediately by hands-on laboratory sessions using a variety of
workstations. Students will "learn-by-doing" with two to a machine. Lab
handouts will describe some specific exercises to be performed on "canned"
data sets. Facilities and supervision will be available until 11:00 PM, for
students to work on their own data.
****************************************
Prof. James B. Pawley, (on Sabbatical) Ph. 61-2-9351-7548/2351
Room LG 10, Madsen Building, F-09, FAX 61-2-9351-7682
University of Sydney, NSW, 2006 Australia JBPAWLEY-at-FACSTAFF.WISC.EDU
Come to the 5th 3D Live-cell Microscopy Course, June 19-29, 2000, UBC, Canada.
Info and forms at: http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
"A scientist is not one who can answer questions but one who can question
answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39



From daemon Fri Feb 04 22:50:55 2000



From: Stan Rice :      srice-at-alpha.utampa.edu
Date: Fri, 4 Feb 2000 21:55:32 -0600
Subject: LKB Nova ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently inherited a used LKB Nova ultramicrotome from a medical
facility in town. The previous owner could not locate the operating
instructions and I am at a loss to figure out its proper operation. Does

anyone on the list have instructions for operation of this instrument??
I would be willing to reimburse anyone for a copy of the instruction
manual.

Dr. Stanley A. Rice
University of Tampa
srice-at-alpha.utampa.edu
(813) 253-3333 Ext. 3340

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From daemon Fri Feb 04 22:50:57 2000



From: Patricia A. Glazebrook :      PGlazebr-at-Research.metrohealth.org
Date: Fri, 4 Feb 2000 21:57:44 -0600
Subject: Cy5 filtercube today on a upright flourescent microscope

Contents Retrieved from Microscopy Listserver Archives
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I tried a Cy5 filtercube today on a upright flourescent microscope equiped
with a Diagnostics Instrument Spot Camera. My tissue had been
immunolabelled with Cy5 and I did not expect to see thru the microscope. So
we took pictures with the Spot Camera and the immunolabeling seemed to
work. The problem was that there was uneven illumination in the pictures,
half moon of brightness and the rest of the field dark. The salesperson did
check that the mercury bulb was aligned correctly. Also there was no
ambient light from the room causing the shadow.

Anyone with an idea  of what is causing this problem?

Thank-you, Pat Glazebrook





From daemon Fri Feb 04 22:50:56 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 4 Feb 2000 21:59:21 -0600
Subject: TEM Specimen Prep short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TEM Specimen Preparation Short Course!!!!

With emphasis on Tripod Polishing and Focused Ion Beam Specimen Preparation
....

..will be offered in conjunction with the Joint Annual Meeting of the
Florida Society for Microscopy and the Florida American Vacuum Society in
Orlando at the University of Central Florida March 15-17, 2000.

Instructors:

Ron Anderson, IBM
Fred Stevie, Lucent Technologies
Lucille Giannuzzi, UCF

Vendor Sponsors:

FEI Company
Micro Optics
South Bay Technology

For registration material please contact Lucille Giannuzzi at lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng.,
University of Central Florida,
PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA
phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu

Director, UCF/Cirent Materials Characterization Facility, 12443 Research
Parkway, Suite 305
Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Sun Feb 06 18:27:19 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 4 Feb 2000 20:52:16 -0800 (PST)
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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The responses to this thread have been outstanding. Yet from my perspective
the unasked and thus unanswerable question is: what is the purpose for the
lens? A couple of the responses have brushed the issue, but it still hasn't
been specifically stated. Assuming that resolution is the main issue, the
concerns of flatness of field, NA, color correction, working distance, etc,
all have to be factored into the evaluation of the different lenses. It
should be possible to demo both (or any of a set of) lenses under the lab
and experimental conditions of real life. Only then can one truly determine
which lens is the "best" (or more correctly, the appropriate) lens. All of
the theory and specifications in the world don't matter if the lens doesn't
aid the microscopist in obtaining the data necessary to carry out the work.
One thing that is unalterable is that even if one is considering a number of
"infinity-corrected" lenses from different manufacturers, they may not be
truly interchangeable. Make sure the lens works on the microscope body.
Then make sure it provides the performance needed. That's the right lens.

Roger Moretz


On Thu, 3 Feb 2000 16:04:55 -0600, Steve Niemela wrote:

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}
} To: {Microscopy-at-MSA.Microscopy.Com}
} Subject: A comparison of optics
} Date: Thu, 3 Feb 2000 10:57:19 -0600
} MIME-Version: 1.0
} Content-Type: multipart/alternative;
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} X-Mailer: Microsoft Outlook Express 5.00.2615.200
} X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200
}
} A client of ours wonders about a comparison between Zeiss and
Olympus
} optics. The Zeiss 100X lense cost $10,000, and the Olympus about
$4,000:
} does that mean the Olympus is inferior?  That's what our client's
} colleagues imply. What measure of lense function is relevant? Does
Olympus
} (or Zeiss) have a specification, like 'resolves particles to 0.1 micron'
} or something like that?  Is there a measure of distortion? Best
} regards, Steve NiemelaVayTek, Inc.305 West Lowe AvenueFairfield, IA
} 52556email: sniemela-at-vaytek.com Phone: 515.472.2227Fax: 515.472.8131web:
} www.vaytek.comgeneral email: vaytek-at-vaytek.com
}
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
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From daemon Sun Feb 06 18:27:43 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 5 Feb 2000 00:36:00 -0800 (PST)
Subject: Re: Aqueous mounting medium

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Lesley,
How about CMC (Refractive Index=1.37). CMC10 (R.I.=1.39), CMCP-9
(R.I.=1.40) and CMCP-10 (R.I. 1.41)? This is a mixture of Polyvinyl
alcohol (5%), phenol(25%), lactic acid (25%), and water (45%).

I believe you can get this from Polysciences Inc. This mountant causes
problems with most stained specimens, but may work for you. Is glycerine
jelly auto-fluorescening?

-Ken

--------------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000N. Willamette Blvd.
Portland, OR 97303





From daemon Sun Feb 06 18:27:45 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 5 Feb 2000 00:45:21 -0800 (PST)
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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Dan,
Mixing and matching objectives is one thing, while doing the same for
oculars is probably not visually as big an issue. However, in highly
corrected systems, compensating eyepieces are a critical consideration if
one wishes to maintain image quality, i.e., plan apo objectives require
compensating oculars to maintain the correction of the apochromatic
objective. This is especially true for color reproduction in color
photomicrograph. This is not as important in black and white
photomicrography.

-Ken

----------------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N Willamette Blvd.
Portland, OR 97303


On Fri, 4 Feb 2000, Dan Freidus wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} -----Original Message-----
} } From: Ken Tiekotter {tiekotte-at-up.edu}
} ..One does not mixed and match objectives from different systems merely for
} the convenience of pricing....
} ----------------------------------------------------------------------------
} ---------------------------------------------------------------------------
}
} Of course, I've heard this sort of statement many times before. But has
} anyone ever done any comparisons of mixed systems to see if there are
} certain brands that are particularly incompatible or particularly
} compatible?
}
} I ask this both as a general question and because I use a Wild M7 for which
} I need a pair of high eyepoint oculars. I don't need the adjusting collar
} built into current models and haven't been able to find an older pair used.
} But before I buy a Nikon/Olympus/ etc. pair I'd love to know how different
} the complete system will be from what I've got now.
}
} I know many people who have used third party photo eyepieces, in just the
} situation where you'd think quality would be of the utmost concern. Dealers
} are often not the best source of info since they tend to represent one brand
} and, even when they are completely honest, rarely have experienc ewith a
} wide range of brands (especially mixed up.
}
} Dan Freidus
} freidus-at-wwnet.com
}
} P.S. Of course, if anyone has a pair of Wild 10x high eyepoint oculars for
} sale, that would also solve my problem (I'd also consider other
} magnifications). (I'm also looking for accessory objective lenses and
} various other accessories. Please contact me off-list if you've got anything
} for sale or trade.)
}
}
}
}
}
}



From daemon Sun Feb 06 18:28:48 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 05 Feb 00 12:50:25 -0800
Subject: RE: Aqueous mounting medium

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Lesley Weston {lesley-at-interchange.ubc.ca}
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From: Paul Webster :      pwebster-at-hei.org
Date: 05 Feb 00 12:50:25 -0800
Subject: RE: Aqueous mounting medium

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: RE: Aqueous mounting medium
Dear Lesley,

You can make a very useful mounting medium from inexpensive ingredients.
The main ingredient is poly(vinyl) alcohol which is soluble in water but will dry without shrinking too much. The water solubility means you can take previously mounted slides, rehydrate them and use them again.

Fading of fluorescent dyes can be retarded by including anti-fade compounds.

You can find the recipe at {http://www.hei.org/htm/moviol.htm} .

Details of anti-fade compounds can be found here (at the bottom of the page) {http://www.hei.org/htm/ifse.htm} .
Ths site is getting a little outdated so if you feel there are details missing then please contact me with your questions.

Many thanks to Heinz Schwarz of Tuebingen for writing these protocols.

Regards,

Paul Webster

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Sun Feb 06 18:29:32 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Sun, 6 Feb 2000 07:21:55 -0500 (EST)
Subject: solid state emitters

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"Dr. Gary Gaugler" {gary-at-gaugler.com}
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I would call this a typical PARTIAL judgment.
What you see is what you get. Thee only way, to the best of my experience,
is to get both objectives, side by side if possible, and to do your own
evaluation. I have honestly doubts concerning those values set on
objectives.
Things change and optic too and sometimes faster that we could expect.
Norm
----- Original Message -----
} From: Dr. Gary Gaugler {gary-at-gaugler.com}
To: MSA listserver {Microscopy-at-sparc5.Microscopy.Com} ; {sniemela-at-vaytek.com}
Sent: Friday, February 04, 2000 5:02 PM


I am interested to know what the present state of the art is re: solid state
electon emitters, total electron beam currents available and also beam
current densities. If anyone could point out a recent review article, I
would appreciate it. Thanks,

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Sun Feb 06 18:29:52 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Sun, 06 Feb 2000 08:43:16 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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In fact, if you're really interested in getting the BEST lens, try at
least three examples of the ones you are interested in . All high NA lenses
must meet some minimal standard but some are significantly better than that
minimum and the only way to find one that is is to try THE lens you are
going to buy - not just an example.

Bill Miller

At 10:04 PM 2/5/00 -0400, Norm wrote:
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From daemon Sun Feb 06 18:31:08 2000



From: VALLARAY-at-aol.com
Date: Sun, 6 Feb 2000 16:10:45 EST
Subject: Re: Electron diffraction simulations

Contents Retrieved from Microscopy Listserver Archives
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Erik,

Do not have difraction sofrware, but here are some URLs where you can
download free simulation software I use for e-beam to solid interactions,
hope it may be useful for you.

Casino is a freeware for monte carlo simulations:
http://www.gme.usherb.ca/casino/

Monte carlo resources:
http://dsa.dimes.tudelft.nl/pattern_definition/monte/monte.html

My FAVORITE monte carlo software:
http://web.utk.edu/~srcutk/htm/simulati.htm

Valery Ray, MSEE,
SEM engineer
Applied Materials
001-208-8413744



From daemon Mon Feb 07 17:07:17 2000



From: Richard Hey :      richardh-at-jeoleuro.com
Date: Sun, 6 Feb 2000 18:32:21 -0600
Subject: Re: Parallel beam

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Hi everybody
While this has been an interesting discussion we seem to have got away from
the original question.

This is the method I use - after of course ensuring normal alignments and
adjustments have been carried out

Insert the objective aperture with a specimen in the beam. Focus the image
of this aperture in Selected Area Diffraction using the first intermediate
lens focus.

Focus the diffraction pattern (i.e. the smallest zero order spot) with the
final condenser lens, this will give a parallel beam incident upon the
specimen.

To test this, remove the objective aperture and insert the largest selected
area aperture. If the beam is parallel moving this aperture will not move
the diffraction spot.


------------------------------------------------------------

Tilt and Shift alignment wobblers in the 2010

Tilt - The purpose of this alignment is to ensure that when the beam is
tilted it doesn't move on the specimen. This is double tilt correction and
depends on using double deflector coils.

Shift - Double shift correction is meant to ensure that when the beam is
shifted it doesn't tilt. The operation of this alignment is dependent upon
the value of the first intermediate lens. The best value for this alignment
is a parallel beam condition as described above. By the very nature of the
beast it will be found to be impossible to perform this alignment correctly
at some CBD conditions. This is meant to be a convergent beam mode after all
not a parallel beam mode.

I understand that this is also referred to as tilt and shift purity.

Richard Hey

The views expressed herein are purely my own and do not reflect those of my
employer

Richard Hey
Technical Support Engineer
Jeol (UK) Ltd
Phone: (44) 1707 377117




From daemon Mon Feb 07 17:07:20 2000



From: Qiaoling Jin :      jinq-at-pilot.msu.edu
Date: Sun, 6 Feb 2000 20:37:18 -0500 (EST)
Subject: Immnogoldlabeling on TEM

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Dear colleagues,
I am now currently using TEM to observe a filamentous structure
so-called hrp pilus produced by plant pathogen, Pseudomonas syringae which is
grown on planta mimic medium agar plates. Even though I could easily find this
structure through directly put the nickel-grid on the bacteria plate for a
certain time,followed by the negative staining, I can't convince other people
to believe it's the structure we are looking for as its size is around
7-10 nm, which is indistinguishable from that of bacterial flagellum.
So we want to label this structure by using the specific antibody against
its major structural protein followed by the 10 nm gold-conjugated second
antibody. The difficulty I am encountering now is most pili are washed away
after the whole immnuogoldlabeling process. Since I am a beginnner on TEM work,
I don't know whether it's a very commonly encountered problem in
immnogoldlabeling experiment or just a very special case happened to me, such
as the pilus i'm interested in is too vulnerable to survive the exclusive wash,
or I didn't master the whole immunogoldlabeling techinique. If it's a common
problem, I hope someone could kindly provide me with your valuable experience
on how to resolve this problem, how we can prevent them to be washed away. If
it's due to my techinical problem, could you please send me a very detailed
immunogoldlabeling protocol.
Any kind of help will be highly appreciated
have a nice week
yours
qiaoling jin
jinq-at-msu.edu






From daemon Mon Feb 07 17:08:36 2000



From: Augusto_A_Morrone-at-notes.seagate.com
Date: Mon, 7 Feb 2000 08:17:13 -0600
Subject: Re: TEM: Jeol 2010 question

Contents Retrieved from Microscopy Listserver Archives
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Jonathan, Andrew, Richard, Larry:

Thank you for your responses. Please note that I do perform the TILT and SHIFT
alignments (aka "pivot point alignment" in other instruments) on a regular
basis, but this doesn't solve the problem.

Regarding: "...What did you mean by a voltage centre? My interpretation of this
is that
the source high tension is wobbled and any shift in the (previously
focussed) image is minimised by tuning the beam tilts. This alignment would
be used for energy filtered TEM (EFTEM) when doing elemental mapping on a
Gatan Imaging Filter (GIF) for example".

This is a (standard) routine alignment for imaging as well. The voltage center
alignment is done by first focusing the specimen at the optimum OL current
(adjusting Z), then adjusting the Tilt (in image mode) and Shift (in diffraction
mode), and finally adjusting the beam tilts to minimize the image shift at the
center of the screen. The problem arises when the Brightness is changed, as is
typically adjusted when optimizing the illumination on the sample to record an
image (intense illumination) versus an SA diffraction pattern (fully overfocused
condenser). Apparently this brightness change affects the voltage center as
evidenced by a shift of the direct beam as that brightness change is effected in
the SAD mode. This shift in the direct beam is equivalent to a beam tilt.

Augusto




From daemon Mon Feb 07 17:08:38 2000



From: L. D. Marks :      ldm-at-apollo.numis.nwu.edu
Date: Mon, 7 Feb 2000 08:30:10 -0600 (CST )
Subject: Re: Parallel beam

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I regret to say that there are some important points which perhaps are
being missed. Classic optics, i.e. most TEM textbooks and introductory
optics texts are for one of two cases:
a) Every point in the condensor aperture (CA) is incoherent (i.e is not
related in phase in a statistical sense) with respect to any other point.
b) Every point in the CA is coherent (i.e. has a fixed phase) with
respect to any other point.

Approximation a) is used in most computer codes for HREM, approximation
b) for STEM.

Alas, both a) and b) are only approximations! Current microscopes,
particularly FEGs (but perhaps others as well) operate under conditions
when neither of the two approximations are valid. While the analytic
theory is well established (see the Chapter in Born and Wolff on
partial coherence), it is not easy and there are no clean solutions
that you can write down. (It can be solved numerically, but this
would require big 4-D FFT's which are beyond most current computers.)

The simplest test is to fully focus the illumination. If the spot
size is A nm, the coherence in the CA is about 1/A nm-1 (which you can
convert to an angle as appropriate). If this is small relative to the
CA radius you are working with case a) above. If it is about the same as the
CA radius you have b). If it is 2-3 times less, neither approximation
is correct!

The moral of the story:
1) If you have a), you can simulate an HREM image with confidence
using any of the available programs. They are WRONG quantitatively
otherwise.
2) If you have a), you can use simple "ray-diagram" optics,
but not otherwise.
3) If you have b) the simple equations for STEM operation are
valid, but not otherwise.

Sorry, but as microscopes improve the theory we need to understand them
can change.

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Mon Feb 07 17:08:40 2000



From: Michael Herron :      herro001-at-maroon.tc.umn.edu
Date: Mon, 07 Feb 2000 09:20:44 -0600
Subject: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
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All,

I am trying to get a handle on the scale of some old pictures. There
are human RBC in theses pictures. Can anyone tell me the diameter of
your average human RBC?

Thanks
Mike

--
_________________________________________________
/ Michael J. Herron /
/ U of MN,Medicine/Infectious Diseases /
/ herro001-at-maroon.tc.umn.edu /
/ http://128.101.243.213 /
/_____________________________________________/



From daemon Mon Feb 07 17:09:09 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Mon, 7 Feb 2000 10:52:29 -0500
Subject: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
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When a characteristic X-ray is given off from an atom, it carries 1 h-bar
(Planck's constant divided by 2 pi) of angular momentum. The electronic
transition that occurred for the X-ray to come off must conserve angular
momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
state is forbidden by selection rules. You can't produce a Be X-ray.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Mon Feb 07 17:09:24 2000



From: A. K. Christensen :      akc-at-umich.edu
Date: Mon, 07 Feb 2000 11:38:52 -0500
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
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Mike: According to Fawcett's CONCISE HISTOLOGY (page 32), "In the blood,
erythrocytes are 8.5 micrometers in diameter, but in the dehydrated state,
in dried blood smears, they measure 7.6 micrometers." --Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Office: 2703A Medical Science II Building
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
E-mail: akc-at-umich.edu
http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
--On Mon, Feb 7, 2000 9:20 AM -0600 Michael Herron
{herro001-at-maroon.tc.umn.edu} wrote:

} All,
}
} I am trying to get a handle on the scale of some old pictures. There
} are human RBC in theses pictures. Can anyone tell me the diameter of
} your average human RBC?
}
} Thanks
} Mike
}
} --
} _________________________________________________
} / Michael J. Herron /
} / U of MN,Medicine/Infectious Diseases /
} / herro001-at-maroon.tc.umn.edu /
} / http://128.101.243.213 /
} /_____________________________________________/






From daemon Mon Feb 07 17:09:45 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Mon, 7 Feb 2000 12:06:10 -0500 (EST)
Subject: Re: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



John, If they do, I hope that they will respond to the whole list because we
have clients who have experienced similar problems, especially re: computers
that are controlling VIS spectrometers collecting CL spectra. I know that
there are in line RF filters available (e.g., Steward Co.) and there may be
others but the final word is not in on how well these work yet.

Don Marshall


} From microprobe-at-microanalysis.org Mon Feb 7 11:43:28 2000
} }
} From: John Hunt {hunt-at-ccmr.cornell.edu}
} Subject: Computer lock-ups from static
} To: microprobe-at-microanalysis.org
}
}
} Dear Probe community,
} It is winter in Central NY, and the room humidity is 33%. Static
} electricity (I believe) has been causing frustrating computer lockups
} requiring rebooting our microprobe workstation.
} I am wondering if anyone has found the best solution for preventing
these
} problems.
}
}
}
}
} John Hunt
} Cornell Center for Materials Research
} 607-255-0108 microprobe
} -3789 SEM/TEM
} -2365 fax
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Mon Feb 07 17:09:49 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Mon, 7 Feb 2000 12:33:38 -0500
Subject: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to tap the wealth of information out there.

We are experiencing a lot of problems with the tungsten filaments that we
are currently using on our JEOL 5800 SEM. During long acquisitions (many
hours) there is a lot of brightness drift and shifting, even when lengthy
stabilization periods are given prior to the acquisitions. JEOL has
checked out the SEM itself, and can't find the source for the drifting. I
was thinking that it might be the filament. I was wondering if there are
different types of tungsten filaments out there, and which is the most
stable over long periods of time. I would like to try out all of them
(considering trying to switch our entire system to a LaB6 filament is a bit
much for now). Any recommendations or experiences you have would help us
out a lot.

Thanks in advance!


Marisa Ahmad
R&D Specialist
Semiconductor Insights
mahmad-at-semiconductor.com


weather report (for those interested):
It's unusually sunny and warm for here in Ottawa.....only -10 degrees C (but
it feels like -25 with the wind). It's such a nice day that I washed my
car this morning since it's not really supposed to be brown - now all the
doors and windows are frozen shut. {sigh}



From daemon Mon Feb 07 17:10:07 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Mon, 7 Feb 2000 12:45:54 -0500
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my experience, RBCs prepared for SEM by critical point drying or HMDS
shrink even more and may be only 4 or 5 microns across.

Marie
}
} Mike: According to Fawcett's CONCISE HISTOLOGY (page 32), "In the blood,
} erythrocytes are 8.5 micrometers in diameter, but in the dehydrated state,
} in dried blood smears, they measure 7.6 micrometers." --Kent
}

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936




From daemon Mon Feb 07 17:10:10 2000



From: Karl E. Garsha :      keg-at-csd.uwm.edu
Date: Mon, 7 Feb 2000 12:03:51 -0600 (CST)
Subject: Re: Immnogoldlabeling on TEM

Contents Retrieved from Microscopy Listserver Archives
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------------- Begin Forwarded Message -------------



------------- End Forwarded Message -------------




From daemon Mon Feb 07 17:10:12 2000



From: Karl E. Garsha :      keg-at-csd.uwm.edu
Date: Mon, 7 Feb 2000 12:10:39 -0600 (CST)
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It depends on the fixation/dehydration parameters. Somewhere in the
nieghborhood of 4 microns, dependant on the angle of measurement and the angle
of the sample with respect to the camera.
} Date: Mon, 07 Feb 2000 09:20:44 -0600
} From: Michael Herron {herro001-at-maroon.tc.umn.edu}
} X-Accept-Language: en
} MIME-Version: 1.0
} To: Microscopy listserv {Microscopy-at-sparc5.Microscopy.Com}
} Subject: Diameter of human RBC?
} Content-Transfer-Encoding: 7bit
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Feb 07 17:10:18 2000



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Mon, 7 Feb 2000 13:25:52 -0500
Subject: TEM: What is Brandes dip?

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Can anyone tell me what a Brandes dip is and how it is done? The only thing
I know is that it can be used to detect virus particles from plant sap but
I can't find any references on it.

Thank You!

Margaret

Margaret Dienelt

Plant Pathologist
Electron Microscopy Lab

Floral and Nursery Plants Research Unit
U.S. National Arboretum/Agricultural Research Service/USDA

B. 010A, Rm. 238, BARC-W
10300 Baltimore Avenue
Beltsville MD. 20705 USA

(301) 504-6097
Fax: (301) 504-5096




From daemon Mon Feb 07 17:10:30 2000



From: Hao Li :      haoli-at-eng.umd.edu
Date: Mon, 07 Feb 2000 13:56:57 -0500
Subject: Simulation of dislocation network

Contents Retrieved from Microscopy Listserver Archives
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Does anybody know any program capable of doing TEM simulation of
dislocation network, e.g., misfit dislocations in plane view TEM? I know
some programs can do one or two dislocations, but what I need is the
simulation for a set of dislocations.
Thanks a lot.

Hao Li



From daemon Mon Feb 07 17:10:31 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Mon, 7 Feb 2000 13:59:53 -0500
Subject: Automatic Microscope and Coherence

Contents Retrieved from Microscopy Listserver Archives
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Hi

It has been very interesting reading about diffraction and electron beams
but it is quite clear that we often give the instrument credit, "the
instrument does it automatically", when credit is not due. Almost every
"automatic" is a designers estimate or better still a calculation, it is a
setting!

After 36 years with TEM I have to say that the only area that the
instrument could set in the SA mode could be the approximate position of
the first image plane. We normally move to the SA mode to free up the
diffraction lens (1st intermediate) to enable it to be focussed on the
diffraction (intermediate) aperture independent of the magnification
system. We would then focus the image inside the aperture with the
objective lens to ensure that the selected area IS the area in the
diffraction pattern. Without this procedure electron rotation and
misalignments could give you a diffraction pattern from an area not in the
centre of the screen! Users are correct in that if they set the eucentric
point or better still, adjust the specimen height to focus at the same
objective lens current value, that done the first image plane will be at
the same point within the diffraction (intermediate) lens.

So how could the Philips work? I would guess they set the diffraction lens
at approximately the first image plane. Then as they do not use it within
their lens scheme for the SA magnifications it is fixed at this focus, good
enough I think?

A second point, parallel beams are very important if you are chasing the
best image quality, biology or materials, most times we need high
coherence. There are misunderstandings on how to obtain a parallel beam or
high coherence, setting the final condenser underfocus is incorrect. The
procedure for high coherence would be to use the smallest spot size you can
tolerate (this probably means you must up the emission current). Once in
this condition overfocus the final condenser (clockwise from crossover) the
spot, what ever size it is now, becomes your virtual source. The further
overfocus you go the greater the beam coherence and that is what we are
after. You will deduce the smaller the condenser aperture the sharper the
spot and the smaller the spot the greater the coherence for a given degree
of overfocus.

Work with a design team and they expect everyone to overfocus, they expect
everyone to use small condenser apertures and they expect everyone to use
small spot sizes. They do not expect everyone to use too low an emission
current (almost everyone does, we have talked before about filament life
being the most important feature of many laboratories) because this makes
the task too difficult!
There must be people more knowledgeable than I who will cut out all the
guesswork?

Steve Chapman
Senior Consultant
Protrain
Electron microscopy courses world wide
http://www.emcourses.com
Tel & Fax 44 (0) 1280 814774



From daemon Mon Feb 07 17:11:18 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Mon, 7 Feb 2000 14:27:14 -0500 (EST)
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mon, 7 Feb 2000, Michael Herron wrote:

} I am trying to get a handle on the scale of some old pictures. There
} are human RBC in theses pictures. Can anyone tell me the diameter of
} your average human RBC?

When I was a lad...... in grad school, we were told it was
a useful reference at 7um diameter.

Kal



From daemon Mon Feb 07 17:11:30 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Mon, 07 Feb 2000 15:15:13 -0500
Subject: ANNOUNCEMENT Course on Polarized Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
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New York Microscopical Society

Bernard Friedman Memorial Workshop

Polarized Light Microscopy

Two consecutive weekends

Saturday, April 21, Sunday, April 22, 2000
Saturday, April 28, Sunday, April 29, 2000

An advanced course on polarized light microscopy which will cover the following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation
The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation

The workshop will consist of two consecutive weekends of lectures and hands on labs to cover
the theoretical and practical aspects of polarized light microscopy.

The course instructors include;

Jan Hinsch of Leica, Inc.,

John Reffner of Trace Consulting,

N.Y.M.S. Instructor Donald O'Leary.

WHEN: April 22, 23, 28 & 29, 2000 from 10 A.M. to 4 P.M.

WHERE: Location To be Announced.

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the Microscope" or
are experienced in microscopy and familiar with the theory of its use.

HOW: Register using the form below. Limited to the first 12 registrants.


FURTHER INFORMATION: Contact Donald O'Leary

eMail: mailto:donoleary-at-worldnet.att.net

(201) 797-8849 Voice Phone Number

PLEASE POST
------------------------------------------------------------------------------------------------------------

Return form to Donald O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

Registration Form

Polarized Light Microscopy, April 22, 23, 28 & 29, 2000

N.Y.M.S. Member _________________ ($275.00) Non-Member __________($295.00)

Name ___________________________________________________________________________

Address _________________________________________________________________________

City _________________________________ State __________________ Zip Code _______________

Phone (W) ____________________________ (H) ___________________________

eMail Address: ______________________________________




________________________________________________________
1stUp.com - Free the Web®
Get your free Internet access at http://www.1stUp.com



From daemon Mon Feb 07 17:12:08 2000



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 7 Feb 2000 13:23:54 -0800 (PST)
Subject: Re: Cy5 filtercube today on a upright flourescent microscope

Contents Retrieved from Microscopy Listserver Archives
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Hi Patricia,

I'm just taking a guess. Is it possible, depending on the type of filters
used in the cube, that infrared is passing through as flare if your first
excitation filter is not blocking the IR? Or there may be a coating
problem on your filter? We have an IR blocking filter right after the
mercury lamp. The CCD cameras are extremely sensitive to IR and this has
caused problems on our system. But in your situation I don't know why it
would pass through. So it is probably something else.

Bob
Morphology core
U of Washington

On Fri, 4 Feb 2000, Patricia A. Glazebrook wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I tried a Cy5 filtercube today on a upright flourescent microscope equiped
} with a Diagnostics Instrument Spot Camera. My tissue had been
} immunolabelled with Cy5 and I did not expect to see thru the microscope. So
} we took pictures with the Spot Camera and the immunolabeling seemed to
} work. The problem was that there was uneven illumination in the pictures,
} half moon of brightness and the rest of the field dark. The salesperson did
} check that the mercury bulb was aligned correctly. Also there was no
} ambient light from the room causing the shadow.
}
} Anyone with an idea  of what is causing this problem?
}
} Thank-you, Pat Glazebrook
}
}
}
}
}



From daemon Mon Feb 07 17:12:10 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 07 Feb 2000 16:26:47 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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One more thought to support Gary's observation about the importance of the
NA of the condenser: The full equation for the limit of resolution is R =
(1.22)(wavelength)/(NAobjective + NAcondenser). However, if you don't oil
it to the back of the slide, you might as well use a lower NA condenser.
Reminds me of the hematology scope built by an otherwise sensible company,
meant to be used in ultra high resolution applications. The manufacturer
was very good about providing a quality, high NA, oil immersion objective
as well as a high NA condenser. The only problem was that you could never
raise the condenser high enough to oil it to the back of the slide and,
therefore, use it at the NA inscribed... only at about .95! What a waste
of time and money!

Also, a reminder that the NAs engraved are the maximum working limit.
Closing down an aperture iris or an iris in the back focal plane of the
objective reduces the NA accordingly.

One last fact: the NA affects both resolution AND edge information, so,
for the crispest, highest resolution images, go for high NA in both
objective and condenser.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 03:02 PM 2/4/00 -0600, Dr. Gary Gaugler wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Feb 07 17:12:14 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 7 Feb 2000 16:35:56 -0500 (EST)
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marisa Ahmad:
} I would like to tap the wealth of information out there.
}
} We are experiencing a lot of problems with the tungsten filaments that we
} are currently using on our JEOL 5800 SEM. During long acquisitions (many
} hours) there is a lot of brightness drift and shifting, even when lengthy
} stabilization periods are given prior to the acquisitions. JEOL has
} checked out the SEM itself, and can't find the source for the drifting. I
} was thinking that it might be the filament. I was wondering if there are
} different types of tungsten filaments out there, and which is the most
} stable over long periods of time. I would like to try out all of them
} (considering trying to switch our entire system to a LaB6 filament is a bit
} much for now). Any recommendations or experiences you have would help us
} out a lot.
}
Dear Marisa,
Do you have control over the filament voltage? If so, set the
voltage just a hair over that necessary to saturate the filament. If
the voltage is too low, parts of the filament will not emit electrons
which get through the wehnelt and into the beam; if too high, there may
be significant evaporation of the filament; either may cause drifting
and/or shifting. I have had very good luck with the stabilities of
both Agar and Ebtec (now Energy Beam Sciences) filaments; not that
others may not be as good, I haven't tried them. Ebtec makes several
shapes of filament, and one may be best for long-term stability. I
have no interest in Agar or Ebtec except as a satisfied customer.
Yours,
Bill Tivol



From daemon Mon Feb 07 17:12:17 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 07 Feb 2000 17:07:29 -0500
Subject: Re: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

Have you considered a "local" remedy, like putting up a humidifier? I know
that static can be an issue, but I have never heard of it being this bad
before.
In more humid environments, we often put a small enclosure around a light
microscope then put out a dish of Drierite. Perhaps some simple plastic
sheeting, like they sell for exterior storm windows/wind breaks, can be
hung from the ceiling around your microprobe plus the humidifier will keep
the humidity immediately around your microprobe more balanced.

Best of luck on a tough problem.

Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 12:06 PM 2/7/00 -0500, donald j marshall wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Feb 07 17:12:25 2000



From: BLADE1691-at-aol.com
Date: Mon, 7 Feb 2000 17:33:41 EST
Subject: pictures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------


how can i get a picture of you seal



From daemon Tue Feb 08 16:19:22 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 7 Feb 2000 14:52:34 -0800 (PST)
Subject: Re: Cy5 filtercube today on a upright flourescent microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is this a Spot Jr. or a SpotII? The IR filter in the Jr. cuts off at 650
nm, the SpotII puts it on a slider. Is it all the way out or [partially
occluding the image? Bob Fern
caught a lot of the possible issues, but is your lamp properly focussed
and aligned? Are you picking up light through the eyepieces? We have a
Nikon that will show a semi-circle of light in from a
recessed ceiling light
that projects to the scope at an angle.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu


On Fri, 4 Feb 2000, Patricia A. Glazebrook wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I tried a Cy5 filtercube today on a upright flourescent microscope equiped
} with a Diagnostics Instrument Spot Camera. My tissue had been
} immunolabelled with Cy5 and I did not expect to see thru the microscope. So
} we took pictures with the Spot Camera and the immunolabeling seemed to
} work. The problem was that there was uneven illumination in the pictures,
} half moon of brightness and the rest of the field dark. The salesperson did
} check that the mercury bulb was aligned correctly. Also there was no
} ambient light from the room causing the shadow.
}
} Anyone with an idea  of what is causing this problem?
}
} Thank-you, Pat Glazebrook
}
}
}
}
}




From daemon Tue Feb 08 16:19:50 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Mon, 7 Feb 2000 16:06:21 -0700
Subject: RE: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Out here in the dry West (here we have Summer humidity around the 33%
value you mention, in Winter we go a lot further down) we have the same
problem. There are a few methods to help:

1) increase humidity. Perhaps you can use a humidifier in your equipment
room?
2) prevent charging. There are shoes available that prevent charging.
3) get rid of charging. There are several options here:

a) The easiest way and what I usually do if no other method is
available, is to touch some metal part NOT of the PC but a chair or
table before touching the PC. This requires a) that a suitable object is
near the PC, b) that you're not afraid of "shocking" yourself, and c)
that you don't move around a lot while working on the PC.

b) Purchase a grounding mat to place in front of the equipment. these
mats are connected to the AC ground (use the same as the PC ground). If
the mat is big enough so that everybody has to step on the mat first AND
is wearing the conductive shoes, everything should be OK.

c) wear grounded wrist straps. These require of course that you put them
on.

We use a combination of these techniques to protect our equipment both
in the office and the production floor (believe me, it IS necessary
here). As bad as it is for electronic equipment, though, it is just
great for comfort levels outside. 90 degrees (F) feel just right for
bicycling, climbing, rafting...

One company where you can buy this stuff is called "-at-once". Their web
site is www.4atonce.com. I just happened to find their catalog first.
There are many other companies out there that sell static control
equipment. We have no financial interest in this company whatsoever.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From: donald j marshall[SMTP:DMRELION-at-WORLD.STD.COM]
} Sent: Monday, February 07, 2000 10:06:10 AM
} To: hunt-at-ccmr.cornell.edu
} Cc: Microscopy-at-sparc5.Microscopy.Com
} Subject: Re: Computer lock-ups from static
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



John, If they do, I hope that they will respond to the whole list
because we
have clients who have experienced similar problems, especially re:
computers
that are controlling VIS spectrometers collecting CL spectra. I know
that
there are in line RF filters available (e.g., Steward Co.) and there may
be
others but the final word is not in on how well these work yet.

Don Marshall


} From microprobe-at-microanalysis.org Mon Feb 7 11:43:28 2000
} }
} From: John Hunt {hunt-at-ccmr.cornell.edu}
} Subject: Computer lock-ups from static
} To: microprobe-at-microanalysis.org
}
}
} Dear Probe community,
} It is winter in Central NY, and the room humidity is 33%.
Static
} electricity (I believe) has been causing frustrating computer lockups
} requiring rebooting our microprobe workstation.
} I am wondering if anyone has found the best solution for
preventing
these
} problems.
}
}
}
}
} John Hunt
} Cornell Center for Materials Research
} 607-255-0108 microprobe
} -3789 SEM/TEM
} -2365 fax
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address.
Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Feb 08 16:19:40 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Mon, 7 Feb 2000 17:24:50 -0600
Subject: GFP antibodies

Contents Retrieved from Microscopy Listserver Archives
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I am planning to try EM immunolabelling with GFP antibodies. Does anyone
out there know of the best source for GFP antibodies and had any luck with
using them for TEM. I would like to try pre-embedding with a nanogold
labelled secondary. Any suggestions would be appreciated. Thank you.

JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856




From daemon Tue Feb 08 16:19:42 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Mon, 07 Feb 2000 19:04:05 -0500
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
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Marisa,

Just a quick comment for whatever it's worth. Your posting suggests that you
are concerned about mechanical drifts of the filament -- the subject of a
certain body of folklore in the microscopy community. It's been my experience
that the principal drift in a tungsten filament is not the filament itself, but
rather it is the mechanism supporting the filament which drifts.

The actual issue with drifting filaments is that mechanical stresses in the wire
will gradually relieve under heat, causing the filament to distort slightly.
This is a first-time-used issue. However, I believe most manufacturers include
an annealing step in their process to remove these stresses before the filament
is shipped to a customer (at least, that's what we used to do in another company
when we manufactured our own filaments). In any case, the filament is operated
at a very high temperature and it doesn't take long to anneal out these stresses
under normal use, so long-term drift effects shouldn't be an issue.

My experience is that long-term mechanical drifts originate elsewhere in the gun
mechanism. When you think about it, this makes sense. The filament itself has
very little mass and quickly reaches its ultimate operating temperature
distribution. However, the rest of the gun assembly is much more massive and,
depending on various design considerations, may take hours to reach thermal
equilibrium. Meanwhile, all of those intricate parts are expanding -- and many
at different rates. Principal suspects, in my opinion, are lateral adjusting
and clamping screws -- for example, a set of radial set screws is commonly used
to center the ceramic base of the filament within a ring of some type. I used
to work with a rather complex assembly which positioned the filament via lateral
screws and sometimes had episodes of "filament drift". Subsequently, I have had
nearly a decade of experience with a very simple design which supports the
filament solely via axial pressure and find that "filament drift" isn't an
issue. My conclusion is that a lot of what I used to call "filament drift" was
actually mechanism drift. So if you are experiencing mechanical instabilities
of the filament, I would suspect the way the filament is being supported more
than the filament itself.

This opinion is based on my own experiences, however, and I will be very
interested to hear if others have had different experience.

Fred Schamber
RJ Lee Instruments Limited

Marisa Ahmad wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would like to tap the wealth of information out there.
}
} We are experiencing a lot of problems with the tungsten filaments that we
} are currently using on our JEOL 5800 SEM. During long acquisitions (many
} hours) there is a lot of brightness drift and shifting, even when lengthy
} stabilization periods are given prior to the acquisitions. JEOL has
} checked out the SEM itself, and can't find the source for the drifting. I
} was thinking that it might be the filament. I was wondering if there are
} different types of tungsten filaments out there, and which is the most
} stable over long periods of time. I would like to try out all of them
} (considering trying to switch our entire system to a LaB6 filament is a bit
} much for now). Any recommendations or experiences you have would help us
} out a lot.
}
} Thanks in advance!
}
} Marisa Ahmad
} R&D Specialist
} Semiconductor Insights
} mahmad-at-semiconductor.com
}
} weather report (for those interested):
} It's unusually sunny and warm for here in Ottawa.....only -10 degrees C (but
} it feels like -25 with the wind). It's such a nice day that I washed my
} car this morning since it's not really supposed to be brown - now all the
} doors and windows are frozen shut. {sigh}



From daemon Tue Feb 08 16:20:58 2000



From: schmutzm-at-lear.u-strasbg.fr (Schmutz Marc)
Date: Tue, 8 Feb 2000 09:27:07 +0900
Subject: Hematology Slide Stainer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi Colleagues around the world,



We're interested in knowing a little bit more about the Hematology Slide
Stainer RSG 61 commercialized by EMS. So if you have one can you please
share your experience with us. Give the plus and also all the minus. If you
have another equivalent brand in which you're fully satisfied of course say
it. Naturally salesman can contact me (offline only please, so the whole
community will not be annoided...)

Thank you for your help


Marc


PS hi Keith how is weather in Plymouth ? Here it's raining and we're singing...





------------------------------
SCHMUTZ Marc
IGBMC
1 rue Laurent FRIES
BP 163
F 67404 Illkirch Cedex
FRANCE

Tel: +33 (0)388 653 330 direct
Fax: +33 (0)388 653 201
email:schmutzm-at-lear.u-strasbg.fr

------------------------------




From daemon Tue Feb 08 16:22:23 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Mon, 07 Feb 2000 16:39:50 -0800
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Nasa has been working on micro-machined solid state electron emitters
and Thomas George of that agency has published a design utilizing an
array of them as the source for a "palm top" SEM in the August, 1998
issue of Nasa Tech Briefs. The proposed SEM relies upon the speculated
use of an electon transparent membrane at the bottom of the lens column
in this sealed, vacuum pumpless system. Resolution is anticipate in the
micron range.

Nasa has also published designs for miniature x-ray tubes in an October
1995 issue of Nasa Tech Briefs.

As far as I know a solid state high energy electron gun exists only as a
Star Wars project.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233



From daemon Tue Feb 08 21:27:03 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Mon, 07 Feb 2000 16:39:50 -0800
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Nasa has been working on micro-machined solid state electron emitters
and Thomas George of that agency has published a design utilizing an
array of them as the source for a "palm top" SEM in the August, 1998
issue of Nasa Tech Briefs. The proposed SEM relies upon the speculated
use of an electon transparent membrane at the bottom of the lens column
in this sealed, vacuum pumpless system. Resolution is anticipate in the
micron range.

Nasa has also published designs for miniature x-ray tubes in an October
1995 issue of Nasa Tech Briefs.

As far as I know a solid state high energy electron gun exists only as a
Star Wars project.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233



From daemon Wed Feb 09 16:49:00 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Mon, 07 Feb 2000 16:39:50 -0800
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Nasa has been working on micro-machined solid state electron emitters
and Thomas George of that agency has published a design utilizing an
array of them as the source for a "palm top" SEM in the August, 1998
issue of Nasa Tech Briefs. The proposed SEM relies upon the speculated
use of an electon transparent membrane at the bottom of the lens column
in this sealed, vacuum pumpless system. Resolution is anticipate in the
micron range.

Nasa has also published designs for miniature x-ray tubes in an October
1995 issue of Nasa Tech Briefs.

As far as I know a solid state high energy electron gun exists only as a
Star Wars project.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233



From daemon Wed Feb 09 16:49:22 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 07 Feb 2000 19:57:13 -0500
Subject: LM: Course reminder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a friendly reminder about the upcoming American Chemical Society
Course, "Applied Optical Microscopy". This course is not just for chemists!

If you need a refresher on a basic technique, need to know more about
interpreting images gathered by a variety of contrast systems (including
Hoffman Modulation Contrast and Polarized light), or are moving more into
digital imaging, this is a great course for you!

Hands-on, 3 days of total immersion, basic principles through advanced
techniques, quantitative polarized light.... and New Orleans, thrown in for
good measure! $895 for ACS members; $995 for non-members (tuition and
materials only).

Visit the MME website for details: {http://MME-Microscopy.com/education}

Best regards,
Barbara Foster
ACS Course Coordinator

Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Tue Feb 08 16:20:32 2000



From: Hay, Karen :      KHay-at-ahs.llumc.edu
Date: Mon, 7 Feb 2000 19:13:42 -0800
Subject: RE: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------



-----Original Message-----
From: Michael Herron [SMTP:herro001-at-maroon.tc.umn.edu]

I am trying to get a handle on the scale of some old pictures.
There
are human RBC in theses pictures. Can anyone tell me the diameter
of
your average human RBC?


Mike-

I have recently subscribed to this list in the hopes of picking up some SEM
pointers as I am beginning to dip my toes into the field. I certainly
can't answer any SEM questions, but I CAN answer your question regarding the
size of human RBCs. On a dried film, normal RBC's have a diameter of
between 7.2-7.9 um. In certain disease states, they can deviate markedly
from this. Microcytic RBC's can be well under 6 um, while macrocytes will
have diameters greater than 9 um.

Good luck in your sizing!

{ {...} }
Karen Hay, MS, MT(ASCP)
Hematology Research, MC 2522
Loma Linda University Medical Center
Loma Linda, CA 92354
Tel: (909) 558-4000 x45350
Fax: (909) 558-4189
Khay-at-ahs.llumc.edu {mailto:Khay-at-ahs.llumc.edu}




From daemon Tue Feb 08 16:20:51 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 7 Feb 2000 23:24:15 EST
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


what is a rbc?
in machining we had a theoretical size referred to by the Dimension of a RCH
thanks Ed Sharpe archivist for SMECC



From daemon Tue Feb 08 16:20:57 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 7 Feb 2000 22:45:24 -0800 (PST)
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,
How about ~7.5 to 8.5um in diameter (depending on fixation/preparation)
and ~2um in greatest thickness.
-Ken

On Mon, 7 Feb 2000, Michael Herron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} All,
}
} I am trying to get a handle on the scale of some old pictures. There
} are human RBC in theses pictures. Can anyone tell me the diameter of
} your average human RBC?
}
} Thanks
} Mike
}
} --
} _________________________________________________
} / Michael J. Herron /
} / U of MN,Medicine/Infectious Diseases /
} / herro001-at-maroon.tc.umn.edu /
} / http://128.101.243.213 /
} /_____________________________________________/
}
}



From daemon Tue Feb 08 16:21:01 2000



From: Bright, Alan :      ABright-at-brightinstruments.com
Date: Tue, 8 Feb 2000 08:59:57 -0000
Subject: SEM: automotive paints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jorgelina,

We have supplied a number of Cryostats & Microtomes to the automotive
industry for sectioning paint finishes on steel body parts & plastic
components e.g. bumper (fenders) door handles, door handle surrounds
etc. Please view our Website to see our range of instruments.

If I can be of further assistance please come back to me.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
} From: "csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com
[mailto:"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com]
Sent: 08 February 2000 17:35
To: MICROSCOPY-at-sparc5.microscopy.com


Hi!
My name is Jorgelina C. Altamirano. I'm an undegrated student of
the
licenciate in chemical sciences.
I'm currently preparing my thesis, being its subjet: The
morphological
characterization and the chemical analysis of automotive paints with
forensic
purposes.
I'm undergoing my work using SEM and EPMA techniques with a
JEOL,
JSM 35C and a microprobe EDAX PV9500.
I've chosen some papers as references:
- Beam et al, Analysis Protocol for discrimination for
automotive
paints by SEM-EDAX, Journal of Forensic Sciences, 35, 5, 1990
- Zieba-Palus et al, Mikrochim. Acta, 14, 1997.

I've taken same samples of automotive paints (such as Fiat,
Peugeot,
Renault, Ford, Chevrolet, Volkswagen) corresponding to the years
1994-99.
I've found many difficulties while preparing them in:
- separating the paint from the body work of the car;
- cutting the specimen;
- to stick specimen to the graphite specimen pedestal;

Though I've made the primary morphological and chemical analysis
and I
haven't observed great differences between the diferent automotive
paints
trades (while polyuretane paints and belayer metalizer paints already
tested),
but I'd found great differences between layers and layers of the paints
specimen.
As I'm not quite satisfied with the results obtained to be used
for
forensic purposes, I would greatly appreciate making contacts with
specialist
fo this subject, so as to receive any comments on this subject.
Thank you in advance for your help.
Jorgelina Altamirano
CERIDE,
Guemes 3450
(3000) Santa Fe, Argentina
e-mail: csedax-at-arcride.edu.ar



From daemon Tue Feb 08 16:21:10 2000



From: mauty-at-MOOREPARK.TEAGASC.IE
Date: Tue, 08 Feb 2000 11:22:33 +0000
Subject: HeNe laser

Contents Retrieved from Microscopy Listserver Archives
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Jorgelina
SEM/EDS//EPMA is only one class of analytical methods that the Forensic
community applies to paint analysis for comparison and association with
original source. Although used routinely, probe methods are not used
exclusively. The organic components in paints are generally more
discriminating.
Sample preparation usually entails some form of embedment and either
microtomy or polishing in order to reveal internal structure.
Removal of paint from an auto body is tricky, and requires practice.The
manufacturers have designed their paint systems to prevent removal!
I would be glad to provide you with additional resources.

Dennis

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net


----- Original Message -----
} From: {"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com}
To: {MICROSCOPY-at-sparc5.Microscopy.Com}
Sent: Tuesday, February 08, 2000 9:35 AM


Jorgelina
SEM/EDS//EPMA is only one class of analytical methods that the Forensic
community applies to paint analysis for comparison and association with
original source. Although used routinely, probe methods are not used
exclusively. The organic components in paints are generally more
discriminating.
Sample preparation usually entails some form of embedment and either
microtomy or polishing in order to reveal internal structure.
Removal of paint from an auto body is tricky, and requires practice.The
manufacturers have designed their paint systems to prevent removal!
I would be glad to provide you with additional resources.

Dennis

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net




----- Original Message -----
} From: "Bright, Alan" {ABright-at-brightinstruments.com}
To: {"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com} ;
{MICROSCOPY-at-sparc5.Microscopy.Com}
Sent: Tuesday, February 08, 2000 12:59 AM


Jorgelina
SEM/EDS//EPMA is only one class of analytical methods that the Forensic
community applies to paint for characterization and association with
original source. Although used routinely, probe methods are not used
exclusively. The organic components in paints are generally more
discriminating.
Sample preparation usually entails some form of embedment and either
microtomy or polishing in order to reveal layer structure.
Removal of paint from an auto body is tricky, and requires practice.The
manufacturers have designed their paint systems to prevent removal!
I would be glad to provide you with additional resources.

Dennis

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net




----- Original Message -----
} From: {"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com}
To: {MICROSCOPY-at-sparc5.Microscopy.Com}
Sent: Tuesday, February 08, 2000 9:35 AM


Dear all

I am planning to replace my Kr/Ar laser on a Zeiss 310 CSLM with two
separate lasers: 488nm Ar ion and HeNe 543nm. Does anyone have
experience of using a HeNe 543? I need this or something similar to
excite Rhodamine for food structure applications. I'd appreciate any
advice.

The reason I'm replacing the mixed gas laser is that has
averaged 25% downtime over the past three years.

Mark

Mark Auty
Dairy Products Research Centre
Moorepark, Fermoy, Co. Cork, Ireland.
mauty-at-moorepark.teagasc.ie
tel 00353 2542447
fax 00353 2542340




From daemon Tue Feb 08 16:21:36 2000



From: Stan Rice :      srice-at-alpha.utampa.edu
Date: Tue, 08 Feb 2000 09:04:43 -0400
Subject: LKB Nova

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Thank you for the many responses to my inquery about operation
instructions for the LKB Nova ultramicrotome. For future reference, I
have the instrument and the original instructions for the LKB Reichert
ultramicrotome if anyone has acquired one of these without instructions.
Thanks again for all of your responses.

Stan Rice
University of Tampa



From daemon Tue Feb 08 16:21:25 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 8 Feb 2000 08:42:57 -0500 (Eastern Standard Time)
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
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On Mon, 7 Feb 2000 12:33:38 -0500 Marisa Ahmad
{mahmad-at-semiconductor.com} wrote:

} We are experiencing a lot of problems with the tungsten filaments that
} we are currently using on our JEOL 5800 SEM. During long acquisitions
} (many hours) there is a lot of brightness drift and shifting, even when
} lengthy stabilization periods are given prior to the acquisitions.
} JEOL has checked out the SEM itself, and can't find the source for the
} drifting

Marisa,

You can easily enough to see if it is filament drift. First, start the
filament alignment program to ensure alignment. Then with the beam on,
wait a few minutes for the filament to drift, then switch off the
autobeam function, and manually check the alignment using the 2
filament alignment knobs. If you can make the image brighter, then
there is filament drift. With my experiences with the 5800 though, I
suspect this is not the case.


W. L. (Buddy) Steffens, Ph.D
Dept. of Pathology
University of Georgia




From daemon Tue Feb 08 16:21:28 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 8 Feb 2000 09:02:01 -0500 (Eastern Standard Time)
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Mon, 7 Feb 2000 16:35:56 -0500 (EST) William Tivol
{tivol-at-wadsworth.org} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}

} Dear Marisa, Do you have control over the filament voltage? If so,
} set the voltage just a hair over that necessary to saturate the
} filament. If the voltage is too low, parts of the filament will not
} emit electrons which get through the wehnelt and into the beam; if too
} high, there may be significant evaporation of the filament; either may
} cause drifting and/or shifting. I have had very good luck with the
} stabilities of both Agar and Ebtec (now Energy Beam Sciences)
} filaments; not that others may not be as good, I haven't tried them.
} Ebtec makes several shapes of filament, and one may be best for
} long-term stability. I have no interest in Agar or Ebtec except as a
} satisfied customer. Yours,
} Bill Tivol


Marisa,

This is a good point that Bill makes...if you are slightly
undersaturated, there will be fluctuations in the rate of electron
emission from the filament. The 5800 automatically aligns and
saturates the filament when the Autobeam mode is selected...and on our
5800, saturation in this mode is a bit low. If you turn off the
autobeam switch and use the filament emission knob to slightly increase
the emission current, this might solve the problem at a cost of only a
small decrease in filament life.



W. L. (Buddy) Steffens, Ph.D
Dept. of Pathology
University of Georgia



From daemon Tue Feb 08 16:21:46 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 8 Feb 2000 09:11:56 -0500
Subject: Re: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



John Hunt wrote:
}
} Dear Probe community,
} It is winter in Central NY, and the room humidity is 33%. Static
} electricity (I believe) has been causing frustrating computer lockups
} requiring rebooting our microprobe workstation.
} I am wondering if anyone has found the best solution for preventing
these
} problems.
}
John we had this same problem for many years while we were using the old
RT-11 based Tracor Northern and Kevex x-ray analyzers. The problem was
especially bad with the TN-5500. This was due to the keyboard with built in
scroll knob. The motion of turning that knob on the keyboard was perfect
for generating a little static. (This is pre- GUI and mouse days, except
for the Mac users!) Because of the low humidity conditions and severe
static accumulation, in winter months we were forced to set the entire
keyboard on a grounded pad. We also attached an antistatic strip to the
front of the keyboard. Because of the nature of the job, and the movement
to and from the computer and the microscope controls, we could not wear the
wrist straps. But with the conductive grounding strip across the entire
front of the keyboard we found that we could very easily get into the habit
of grounding our wrist or thumb on the strip before touching the rest of the
computer or keyboard. Then as we used the keyboard we would make some
effort to touch the grounding strip from time to time. This virtually
eliminated the problem. By the way I believe it was such a problem that
Tracor engineered a static discharge switch into the keyboard connection.


Brad Huggins
BPAmoco,
Naperville, Microscopy and Microanalysis



From daemon Tue Feb 08 16:21:58 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 8 Feb 2000 12:44:34 -0400
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 9:20 AM -0600 2/7/0, Michael Herron wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Feb 07 17:12:19 2000



From: csedax-at-alpha.arcride.edu.ar
Date: Mon, 7 Feb 2000 17:36:17 -2359
Subject: SEM: automotive paints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
My name is Jorgelina C. Altamirano. I'm an undegrated student of the
licenciate in chemical sciences.
I'm currently preparing my thesis, being its subjet: The morphological
characterization and the chemical analysis of automotive paints with forensic
purposes.
I'm undergoing my work using SEM and EPMA techniques with a JEOL,
JSM 35C and a microprobe EDAX PV9500.
I've chosen some papers as references:
- Beam et al, Analysis Protocol for discrimination for automotive
paints by SEM-EDAX, Journal of Forensic Sciences, 35, 5, 1990
- Zieba-Palus et al, Mikrochim. Acta, 14, 1997.

I've taken same samples of automotive paints (such as Fiat, Peugeot,
Renault, Ford, Chevrolet, Volkswagen) corresponding to the years 1994-99.
I've found many difficulties while preparing them in:
- separating the paint from the body work of the car;
- cutting the specimen;
- to stick specimen to the graphite specimen pedestal;

Though I've made the primary morphological and chemical analysis and I
haven't observed great differences between the diferent automotive paints
trades (while polyuretane paints and belayer metalizer paints already tested),
but I'd found great differences between layers and layers of the paints
specimen.
As I'm not quite satisfied with the results obtained to be used for
forensic purposes, I would greatly appreciate making contacts with specialist
fo this subject, so as to receive any comments on this subject.
Thank you in advance for your help.
Jorgelina Altamirano
CERIDE,
Guemes 3450
(3000) Santa Fe, Argentina
e-mail: csedax-at-arcride.edu.ar



From daemon Tue Feb 08 16:22:08 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 08 Feb 2000 09:49:38 -0800
Subject: Re: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott,
I've seen a Be Ka x-ray peak in a Link advertisement and we have generated
one in the Quartz XOne applications lab with a really good light element
deteector. You will find the Be Ka1 peak listed at 0.110 keV in most x-ray
tables.
At 10:52 AM 2/7/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Feb 08 16:21:53 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Tue, 08 Feb 2000 09:56:35 -0800
Subject: Desmosomes

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we are looking at desmosomes in mouse epidermis. My question: From
looking at sections we got the impression that demosomes are rather
uniform, round knob-like structures. As we did not do any serial
sections, we are not sure if this is true. Does anybody know of a
publication dealing with this?

Thanks for your help,

Christoph



Christoph Bauer Ph.D.
University of Chicago
Molecular Genentics and Cell Biology
5841 S. Maryland Ave/MC 1028
Chicago, Il 60637



From daemon Tue Feb 08 16:22:09 2000



From: Ingber, Bruce F. :      bingber-at-commserver.srrc.usda.gov
Date: Tue, 8 Feb 2000 11:56:46 -0600
Subject: Re: FEI/Philips CM12 SAD focus

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I received the following fax from Ms. Irene Piscopo, EM Consultant,
regarding electron diffraction procedures for the CM-12 or CM-120 TEM's from
FEI/Philips. I'll copy the information for those interested.

ELECTRON DIFFRACTION PROCEDURES

There are three methods for doing electron diffraction in the CM-12
involving two different techniques: Method I is an aperture limiting method
while Methods II and III are probe limiting. In all cases, the sample must
be in the eucentric position and focused.

METHOD I: (area } 1 µm) SAD

1. Select the SA mode (the diffraction aperture and image are focused in the
same plane).
2. Insert and center the SA aperture around the area of interest.*
3. Select D.
4. Remove the objective aperture.
5. Overfocus the second condenser to sharpen the pattern (i.e. parallel
illumination conditions).
6. Focus the pattern with the focus control which is now changing the
diffraction lens).
7. Change the size of the pattern (i.e. camera length) by changing the
magnification.

Size of the SA aperture
* Size of area selected =
----------------------------------------------------------------------------
------------
Objective lens magnification in D aperture
plane (~ 30x**)

** Actual magnification is focal length dependent.

METHOD II: (areas down to 40 nm with w filament; 20 nm with LaB6)

1. Locate an area of interest.
2. Remove the objective aperture.
3. Using Condensers 1 and 2, bring the beam down to a size slightly smaller
than the area of the area from which the diffraction pattern is to be
obtained.
4. Go to D and select the desired CL with the magnification control.
5. Focus the pattern with the focus control. Note: While it's sometimes
difficult to determine exact focus, it's often easier to insert an objective
aperture and focus the edge of the aperture to determine diffraction focus.
6. The size of the discs within the pattern can be decreased by decreasing
the size of the condenser aperture. Be sure the aperture is well-centered.

METHOD III: NANOPROBE and/or STEM (to 2 nm)

For diffraction and/or chemical information in the TEM mode from
regions of the sample less than 40 nm in diameter requires operating the
instrument in either the NANOPROBE or STEM modes.

NANOPROBE:

1. Depress NANOPROBE.
2. Follow instructions in Method II.

STEM:

1. Obtain a focused STEM image using the smallest C2 aperture.
2. Stop the scan.
3. Lower the main screen (CBED pattern)
4. Slightly larger probes and more parallel beam conditions can be obtained
using UUD and focusing with INTENSITY (C2 lens).

NOTES ON ELECTRON DIFFRACTION

1. The sample must always be eucentric and focused.
2. In METHOD I, the area from which the diffraction pattern is obtained is
determined by the selected area - (diffraction) aperture, when in SA mode.
To sharpen the ED pattern, one overfocuses the second condenser (C2)
3. In METHODS II and III, the area from which the diffraction pattern is
selected depends on the size of the beam. The size of the beam can be
altered by changing condenser lenses C1 and C2 (i.e. spot size and
intensity. To sharpen the ED pattern , one reduces the size of the C2
aperture.
4. Spherical aberration limits METHOD I to ~1 µm.
5. In METHODS II and III, spherical aberration of the objective lens is not
the limiting factor, the minimum probe size is.
6. For identifying an ED pattern, at least three rings are required.
7. Be sure the sample and the standard are in the eucentric position.
Changes in the objective current will cause variations in CL.


Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124

(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-nola.srrc.usda.gov




From daemon Tue Feb 08 16:22:11 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 8 Feb 2000 12:57:15 -0500
Subject: RE: Be X-ray peaks -I don't think so

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I'm wrong. I guess I should have looked up at the X-ray periodic table
above my head and saw the 0.108 keV value for Be. The reason that I was
wrong was that I did not consider the solid state aspects. I am still right
about not having a 2s1/2 to a 1s1/2 transition and that an X-ray carries
away 1h-bar of angular momentum. What I didn't do was think about the band
structure of a solid. If you have the solid, the 2p bands of Be most be
spilling into or be the conduction band for the electrons. That must be the
source for the electronic transition. I guess I should have engaged brain
before typing.
My most humble apologies.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--

} -----Original Message-----
} From: microprobe [mailto:chris.salter-at-materials.oxford.ac.uk]
} Sent: Tuesday, February 08, 2000 7:10 AM
} To: Walck. Scott D.
} Subject: Re: Be X-ray peaks -I don't think so
}
}
} Dear Walck,
}
} You may not, in theory, be able to produce Be X-rays, but we
} can detect them on our JEOL JXA 8800, they correspond to the position
} in the tables for Be Ka!, and the target is pure Be.
}
} On Mon, 7 Feb 2000 10:52:29 -0500 "Walck. Scott D." {walck-at-ppg.com}
} wrote:
}
} -----Original Message-----
} From: Ekstrom, Harry [mailto:harry.ekstrom-at-honeywell.com]
} Sent: Monday, February 07, 2000 7:40 PM
} To: 'Walck. Scott D.'
} Subject: RE: Be X-ray peaks -I don't think so
}
}
} I don't agree Scott. An L or M line for any element would
} not be located in
} the area
} of Be Ka. And the peak I normally get there with an ultra thin window
} is a well defined peak with great resolution.
} Please re evaluate your theory.
}
} Harry Ekstrom
} } }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
} }
}
} --------------------------------------------------------------
} ---------.
} }
} }
} } When a characteristic X-ray is given off from an atom, it
} carries 1 h-bar
} } (Planck's constant divided by 2 pi) of angular momentum.
} The electronic
} } transition that occurred for the X-ray to come off must
} conserve angular
} } momentum of the system. Therefore, a transition from a
} 2s1/2 to a 1s1/2
} } state is forbidden by selection rules. You can't produce a
} Be X-ray.
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } PPG Industries, Inc.
} } Glass Technology Center
} } Guys Run Rd. (packages)
} } P. O. Box 11472 (letters)
} } Pittsburgh, PA 15238-0472
} }
} } Walck-at-PPG.com
} }
} } (412) 820-8651 (office)
} } (412) 820-8161 (fax)
} }
} }
} }
}
} -------------------
} microprobe
} chris.salter-at-materials.ox.ac.uk
}
} * This e-mail message was sent with Execmail V5.0.x *
}



From daemon Wed Feb 09 23:52:18 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Tue, 8 Feb 2000 15:31:08 -0500
Subject: Diffraction & Coherence

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Hi

It has been very interesting reading about diffraction and electron beams
but it is quite clear that we often give the instrument credit, "the
instrument does it automatically", when credit is not due. Almost every
"automatic" is a designers estimate or better still a calculation, it is a
setting!

After 36 years with TEM I have to say that the only area that the
instrument could set in the SA mode could be the approximate position of
the first image plane. We normally move to the SA mode to free up the
diffraction lens (1st intermediate) to enable it to be focussed on thefirst
image plane and the diffraction (intermediate) aperture, independent of the
magnification system. We would then focus the image inside the aperture
with the objective lens to ensure that the selected area IS the area in the
diffraction pattern. Without this procedure electron rotation and
misalignments could give you a diffraction pattern from an area not in the
centre of the screen! Users are correct in that if they set the eucentric
point or better still, adjust the specimen height to focus at the same
objective lens current value, that done the first image plane will be at
the same point within the diffraction (intermediate) lens.

So how could the Philips work? I would guess they set the diffraction lens
at approximately the first image plane. Then as they do not use it within
their lens scheme for the SA magnifications it is fixed at this focus, good
enough I think?

A second point, parallel beams are very important if you are chasing the
best image quality, biology or materials, most times we need high
coherence. There are misunderstandings on how to obtain a parallel beam or
high coherence, setting the final condenser underfocus is incorrect. The
procedure for high coherence would be to use the smallest spot size you can
tolerate (this probably means you must up the emission current). Once in
this condition overfocus the final condenser (clockwise from crossover) the
spot, what ever size it is now, becomes your virtual source. The further
overfocus you go the greater the beam coherence . You will deduce the
smaller the condenser aperture the sharper the spot and the smaller the
spot the greater the coherence for a given degree of overfocus.

Work with a design team and they expect everyone to overfocus, they expect
everyone to use small condenser apertures and they expect everyone to use
small spot sizes. They do not expect everyone to use too low an emission
current (almost everyone does, we have talked before about filament life
being the most important feature of many laboratories) because this makes
the task too difficult!


Steve Chapman
Senior Consultant
Protrain
Electron microscopy courses world wide
http://www.emcourses.com
Tel & Fax 44 (0) 1280 814774



From daemon Tue Feb 08 16:22:19 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Tue, 8 Feb 2000 12:42:13 -0800
Subject: RE: HeNe laser

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} Dear all

} I am planning to replace my Kr/Ar laser on a Zeiss 310 CSLM with two
} separate lasers: 488nm Ar ion and HeNe 543nm. Does anyone have
} experience of using a HeNe 543? I need this or something similar to
} excite Rhodamine for food structure applications. I'd appreciate any
} advice.

} The reason I'm replacing the mixed gas laser is that has
} averaged 25% downtime over the past three years.

} Mark

} Mark Auty
} Dairy Products Research Centre
} Moorepark, Fermoy, Co. Cork, Ireland.
} mauty-at-moorepark.teagasc.ie
} tel 00353 2542447
} fax 00353 2542340


I have been using the Argon ion (488nm) and HeNe 543nm combination on
different Zeiss LSM310's and 410's for the past 9 years. The HeNe 543nm is
fairly low power, but it has been perfectly adequate for scanning the
fluorochromes I have needed to see including TRITC, Texas Red, Cy3,
propidium iodide, and Sirius Red. I have never had a problem with the laser
going down either, but one would expect that with a HeNe laser.

Slàinte,

Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu



From daemon Tue Feb 08 16:22:25 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Tue, 08 Feb 2000 14:06:58 -0800
Subject: cryo chamber for LRWhite UV

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Hi All,
Does anyone have experience with the Pelco UVC2 or UVC1 Cryo Chamber for UV
curing of resins (especially LR White) or with something similar? We're
contemplating buying something like that for UV polymerization of LR White
at -20 C.
Thanks for your help,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Tue Feb 08 21:26:51 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Tue, 08 Feb 2000 17:09:09 -0500
Subject: Re: Be X-ray peaks -I don't think so

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Scott,
I'm not sure what brought this up, but my reaction is "thems fightin'
words". I do not currently have a wavelength reflecting crystal to measure
that low in the periodic table, but I did on another instrument. I distinctly

recall seeing a pretty hefty peak at the Be K-alpha position when I focused
the beam down on a piece of pure Be metal. I guess rules are made to be
broken. Are your calculations correct? Or am I misunderstanding something
here?

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777

"Walck. Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} When a characteristic X-ray is given off from an atom, it carries 1 h-bar
} (Planck's constant divided by 2 pi) of angular momentum. The electronic
} transition that occurred for the X-ray to come off must conserve angular
} momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
} state is forbidden by selection rules. You can't produce a Be X-ray.
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)



From daemon Tue Feb 08 21:26:57 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:04 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:07 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:08 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:10 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:17 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:39 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:49 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:51 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:57 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:49:13 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 23:52:17 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:26:55 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 8 Feb 2000 16:24:58 -0600
Subject: Be X-ray and filament drift

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Dear Listers,
I have responses to two of today's postings, but they are
quite unrelated.
1. I did not see the original question about Be x-rays,
only Scott Walk's reply, so I don't know what info was
being sought. We used a Be-containing mineral to evaluate
microprobes before buying. I won't embarrass those who
failed, but only one manufacturer succeeded. The reason
the others failed is probably that in compounds the x-ray
energy is subject to largish chemical shifts relative to
pure Be because there is no shielding by outer electrons
and you need to search on either side of the tabulated
value of about 0.1keV. So there IS a Be x-ray and a
reasonable WDS spectrometer should detect it.

2. Filament drift. Fred Schamber's reply is right most of
the time, but I did once have a batch of 6 JEOL-type
filaments made by an EM supply house where 2 of them
drifted until the tips were right at the edge of the hole
and stayed there even when the filament was cooled down
again. This was the only time in about 15 years of using
JEOL microscopes.

Eric
----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk




From daemon Tue Feb 08 21:26:54 2000



From: Dean Armytage :      hillstream-at-hypermax.net.au
Date: Tue, 8 Feb 2000 16:27:01 -0600
Subject: Live Blood Staining

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Hi List I am using live blood staining light microscopy, is there
anyone who has tried or is using this technique. I would like to swap
notes. Dean Armytage PhD Hillstream Health Centre Australia




From daemon Tue Feb 08 21:27:20 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 8 Feb 2000 18:55:31 -0700
Subject: Re: Be X-ray peaks -I don't think so

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I'm getting more curious as I read on here. Speaking strictly energy now
and leaving out wavelength, the characteristic energy of Be Ka is about .11
KeV like Mary stated. I've had an occasional peak show up there before and
thought it may have been Be. Now I understand that it may have been "noise"
all along. Would "noise" be a pretty defined and relatively well resolved
peak at that energy level using an ultra thin window? Depending on the low
energy cutoff setting, one might truly have Be and never detect it. Would
lowering the KV reduce the noise artifact?

I guess I'll get out my Be planchet and try a few things.

Harry

-----Original Message-----
} From: Jim McGee [mailto:mcgee-at-geol.sc.edu]
Sent: Tuesday, February 08, 2000 3:09 PM
To: 'Microscopy'


Scott,
I'm not sure what brought this up, but my reaction is "thems fightin'
words". I do not currently have a wavelength reflecting crystal to measure
that low in the periodic table, but I did on another instrument. I
distinctly

recall seeing a pretty hefty peak at the Be K-alpha position when I focused
the beam down on a piece of pure Be metal. I guess rules are made to be
broken. Are your calculations correct? Or am I misunderstanding something
here?

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777

"Walck. Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} When a characteristic X-ray is given off from an atom, it carries 1 h-bar
} (Planck's constant divided by 2 pi) of angular momentum. The electronic
} transition that occurred for the X-ray to come off must conserve angular
} momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
} state is forbidden by selection rules. You can't produce a Be X-ray.
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)



From daemon Wed Feb 09 23:52:21 2000



From: Hong Yi :      hyi-at-emory.edu
Date: Tue, 8 Feb 2000 21:26:04 -0500 (EST)
Subject: Re: New Gold Enhancement Reagents

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Dear Michael:

My personal experience with silver enhancement dates back to when Janssen
Life Sciences first introduced the IntenSE M silver enhancement kit in
late 80's (this product was later taken over by Amersham). At the time, I
felt the IntenSE M was easy to use, but its fast reaction made it hard to
control the particle growth. Just like you, the desire for a better
results has kept me searching and testing whenever a new protocol or
product became available.

Danscher's method gives wonderful intensification, but its low pH
sometimes damages the ultrastructure when used for the pre-embedding
immunogold labeling. Burry's method and the Nanoprobes HQ silver made
progress with pH, however they inherited Danscher's high viscosity and
light sensitivity, which limit their penetration in pre-embedding silver
enhancement and render it more difficult to handle from a practical
standpoint. I have also tested the Nanoprobes GoldEnhance kit for
pre-embedding immunogold labeling recently. At the LM level the results
were decent. I have not spent a lot of time evaluating its performance
at the EM-level. So far, the particle size homogeneity is not as good as
what I've found with Danscher's method. But I will refrain from further
judgment until more work has been done.

In my opinion, the first breakthrough in intensifying gold particles since
Danscher's method was realized when Aurion, a Dutch company, first
introduced the R-gent SE-EM silver enhancement kit last June. As you
wished for in your post, it has a near-neutral pH, low viscosity, and it
is light insensitive. Moreover, it gives a great enhancement efficiency
and even particle size and shape. Background remains minimal even after
two hours enhancement on the bench. Morphology preservation after
enhancement is superior to any other enhancement reagent I have used. I
have been testing this kit profusely for both post- and pre-embedding
immunogold labeling on various types of samples (including cell cultures
and vibratome sections), with many different primary and secondary
antibodies, and am very pleased with its performance. I use 0.5% OsO4 for
20 min and have never had any problem with silver disappearing. If you
like, I would be very happy to e-mail some images to you so you can
evaluate them yourself.

Hong
=================
Hong Yi
Emory University
Department of Neurology
6215 Woodruff Memorial Research Building
1639 Pierce Dr.
Atlanta, GA 30341
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu

On Thu, 3 Feb 2000, Michael Plociniak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
} Has anyone used gold enhancement reagents from Nanoprobes, Inc.
} (Stonybrook, NY - USA) as an alternative to silver enhancement for
} enlarging 1 nm immunogold probes?
}
} Advertisements state that gold enhancement has lower background and is
} compatible with osmium. Can anyone verify this from personal experience?
}
} In addition to these advantages, I am hoping that milder pH conditions will
} be less damaging to ultrastructure in cultured neurons (using a
} preembedment protocol).
}
} Thank you,
} Michael
}





From daemon Wed Feb 09 23:52:29 2000



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Wed, 9 Feb 2000 12:01:47 +0900
Subject: Finding the diffraction plane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody,

Here is the way I found yesterday for finding the diffraction plane. By
diffraction plane I mean the plane at which diffraction pattern is formed
(crossover, Fourier of the object wave) not the theoretical BFP of the
objective.

First perform standard alignment for brightfield mode. Then go to 10 000X.
There are several ways to perform:

1. Fixed illumination and specimen position (i.e. OL current)

a) Adjust the desired brightness with C2. Adjust the desired OL current and
specimen position.
b) Insert OL aperture and move it so that the edge of the aperture to cross
through the middle of the screen. If the aperture is exactly at the diff.
plane then you'll not be able to see its edge - just the whole beam will
fade uniformly ... so you have the diff. plane.
c) If you see aperture edge then it is not at diff. plane. Now two ways:
- change the aperture z-position (not recommended ... but if it is very far
..)
- If your microscope has condenser minilens you can try tweaking both C2 and
CML in order to keep the intensity same and just make the aperture edge
disappear (i.e. the whole screen uniformly darkened).

2. Fixed specimen (i.e. OL current).

a) Focus the specimen
b) same as 1b
c) Change C2 excitement in order to make the edge disappear

3. Fixed illumination

a) Set desired C2 excitement
b) same as 1b
c) Change the objective lens current until the aperture edge disappears.
Then move specimen in z-direction until it is focused.


In all these methods after adjusting the aperture at diff. plane you can try
tweaking C2 or OL ... you will see that the aperture is not at diff. plane
anymore, The edge will appear on one or the other side (i.e. mirrored)
depending on the direction of the lens current change.
Another thing - after adjusting the aperture at diff. plane you can check if
beam shift tilts the beam (by tilting here I mean - if the specimen is
illuminated with plane wave then shifting the beam should not tilt the wave
front). Shift the beam and if the brightness changes (when the aperture edge
partially covers the zero diff. spot) then the beam is tilting.
I wish there was a IL1 tweaking knob allowing for change of the IL1 current
while in brightfield mode. This wold make the things much more easier.

About the methods for making the illumination at the specimen parallel. They
will work only if:
- The OL aperture is positioned by the manufacturer exactly at the
theoretical BFP (I think that in most of the TEMs it is not)
- The OL current should be set to zero deviation (i.e. at the value for
which the BFP position was calculated)
- The intermediate lens system should be properly calibrated by the
manufacturer so that if both of the above conditions are satisfied and the
specimen is at front focal plane of the OL then it to be in focus. In other
words the theoretical BFP of the objective to coincide with the theoretical
front focal plane of the IL1.

I don't think parallel illumination is so very important ... using spherical
wave will give the same results but just the diff. planes will be shifted.

All of the above is just my opinion. I could be mistaking somewhere ...

Best wishes,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------



From daemon Wed Feb 09 16:48:37 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 8 Feb 2000 21:31:38 -0600
Subject: Re: automotive paints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Dennis Ward" {DCWard-at-concentric.net}
}
} Jorgelina
} SEM/EDS//EPMA is only one class of analytical methods that the
Forensic
} community applies to paint analysis for comparison and association with
} original source. Although used routinely, probe methods are not used
} exclusively. The organic components in paints are generally more
} discriminating.
} Sample preparation usually entails some form of embedment and either
} microtomy or polishing in order to reveal internal structure.
} Removal of paint from an auto body is tricky, and requires
practice.The
} manufacturers have designed their paint systems to prevent removal!
} I would be glad to provide you with additional resources.

I have never do this but this is what i would try first.

One thing I would try on would be removing the metal from the paint. A
weak elecrolite solution and a low DC voltage connected to reverse
electoplate the metal away sould get rid of almost all of it and the last
bit could be removed with acid or evaporated as the cathode in a vacum
chamber.

Once you get the metal down to a bunch of small islands you might be able
to let it rust free of the paint.

I have no idea what this would do to the paint but most paint films I have
delt with are pretty tough.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Feb 09 16:48:56 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Wed, 09 Feb 2000 09:43:03 +0100
Subject: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.

Specimen preparation is of course a crucial point of our work.

We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.

We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.

Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.

Thanks.

Massimo




Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm




From daemon Wed Feb 09 23:52:27 2000



From: flaitz-at-us.ibm.com
Date: Wed, 9 Feb 2000 07:48:09 -0500
Subject: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Massimo,

in our lab we have prepared a number of GaAs, AlGaAs, InGaAs devices.
Mechanical thinning is by the tripod wedge technique, but typically we end
up with more damage than we find for Si samples so there is a need to leave
samples thicker and use more extensive ion milling for final thinning.

We also have a PIPS and I have found its capabilities essential for
achieving good thin samples in these materials. I found more success by
using low angles and low KeV for long times. Typically I would be using
5-6 degrees and 4-4.5 KeV initially, with 3-4 degrees and 3-3.5 KeV for the
final ion polishing. On samples 2-3 microns thick, this would amount to
2-3 hours total milling time. I did not observe any artefacts introduced
with milling under these conditions, and the junction structures and
inherent defects were clearly observed.

The conditions above should work with any ion miller capable of low angle
milling, but our experience is only with the PIPS. If you use the wedge
technique for mechanical thinning, I would recommend that you use Mo grids
to mount your specimens as the extended milling times at low angles will
really chew up a Cu grid. Also, Mo grids are considerably thinner,
allowing angles as low as 3-4 degrees on the grid hole side for a sample
positioned in the middle of a 1 mm grid hole.

If you would like, I can send you some typical images of devices we have
prepared, including images showing the removal of the mechanical polishing
damage.


Philip L. Flaitz
IBM Analytical Services, Hopewell Junction, NY
http://www.chips.ibm.com/services/asg
Ph.......(914) 892-3094, FAX -2003
flaitz-at-us.ibm.com



Massimo Catalano {massimo.catalano-at-ime.le.cnr.it} on 02/09/2000 03:43:03 AM

To: Microscopy-at-sparc5.Microscopy.Com
cc:


Dear Listservers,

we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.

Specimen preparation is of course a crucial point of our work.

We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.

We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.

Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.

Thanks.

Massimo




Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm








From daemon Wed Feb 09 16:49:23 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Wed, 9 Feb 2000 13:10:00 +0000
Subject: looking for ETEC Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello .....
we have an old ETEC Omniscan SEM, and we are searching for ETEC company or any
people that can supply manuals ....

any help is welcome

best regards


===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Wed Feb 09 16:49:27 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Wed, 09 Feb 2000 08:11:32 -0600
Subject: autofluorescence quenching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am working with archival specimens of formalin-fixed paraffin embedded
tissues and I am getting tremendous autofluorescence. I looked at tissue
sections after the sections have been de-waxed using either FITC or
rhodamine filter sets and red cells and connective tissues are brightly
fluorescent. Is there a way of suppressing the autofluorescence and still
retain reactivity of tissue to antibodies? I would appreciate any comments
or suggestions.

*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Wed Feb 09 16:49:25 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Wed, 09 Feb 2000 10:26:27 -0500
Subject: Re: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Harry,

I don't have a UTW detector here, so I'll leave this to the EDS experts. But,
on the JEOL 8800/8900 I used to run we had a thin window detector that could see
B (not Be) if the low end noise peak (which is huge) was properly
discriminated. Get out your C planchett while you are getting the Be one, and
see if you get the noise peak at Be. Far better to use wavelength than EDS down
at that end.

Jim McGee
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610

"Ekstrom, Harry" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm getting more curious as I read on here. Speaking strictly energy now
} and leaving out wavelength, the characteristic energy of Be Ka is about .11
} KeV like Mary stated. I've had an occasional peak show up there before and
} thought it may have been Be. Now I understand that it may have been "noise"
} all along. Would "noise" be a pretty defined and relatively well resolved
} peak at that energy level using an ultra thin window? Depending on the low
} energy cutoff setting, one might truly have Be and never detect it. Would
} lowering the KV reduce the noise artifact?
}
} I guess I'll get out my Be planchet and try a few things.
}
} Harry
}
} -----Original Message-----
} } From: Jim McGee [mailto:mcgee-at-geol.sc.edu]
} Sent: Tuesday, February 08, 2000 3:09 PM
} To: 'Microscopy'
} Subject: Re: Be X-ray peaks -I don't think so
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Scott,
} I'm not sure what brought this up, but my reaction is "thems fightin'
} words". I do not currently have a wavelength reflecting crystal to measure
} that low in the periodic table, but I did on another instrument. I
} distinctly
}
} recall seeing a pretty hefty peak at the Be K-alpha position when I focused
} the beam down on a piece of pure Be metal. I guess rules are made to be
} broken. Are your calculations correct? Or am I misunderstanding something
} here?
}
} Jim
} --
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} James J. McGee (email: jmcgee-at-sc.edu)
} Department of Geological Sciences
} University of South Carolina
} Columbia, SC 29208
}
} Tel: 803-777-6300 Fax: 803-777
}
} "Walck. Scott D." wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } When a characteristic X-ray is given off from an atom, it carries 1 h-bar
} } (Planck's constant divided by 2 pi) of angular momentum. The electronic
} } transition that occurred for the X-ray to come off must conserve angular
} } momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
} } state is forbidden by selection rules. You can't produce a Be X-ray.
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } PPG Industries, Inc.
} } Glass Technology Center
} } Guys Run Rd. (packages)
} } P. O. Box 11472 (letters)
} } Pittsburgh, PA 15238-0472
} }
} } Walck-at-PPG.com
} }
} } (412) 820-8651 (office)
} } (412) 820-8161 (fax)

--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610




From daemon Wed Feb 09 16:49:30 2000



From: ipaul-at-MtRoyal.AB.CA
Date: Wed, 09 Feb 2000 09:26:15 -0700
Subject: Sarcomere cross-sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for TEM images that show cross-sectional views of skeletal muscle
sarcomeres during the *contracted state*. In particular, I am interested in
seeing the spacing of the thin filaments when they **overlap each other** (i.e.,
when those from one side of the sarcomere overlap those from the other side of
the sarcomere in the contracted state). In addition, I am interested in seeing
the distribution of the thin filaments as they pass through the M line. It
would be greatly appreciated if anyone could tell me if they know of any
research papers or review articles that contain such TEM images.

Thank you!

Izak Paul
Biological Sciences
Mount Royal College




From daemon Wed Feb 09 23:51:48 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Wed, 9 Feb 2000 13:03:36 -0500
Subject: Paultek camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know if Paultek Imaging still exists and a phone number for
them (or Email)? Thanks-Dave


************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Wed Feb 09 16:49:32 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 9 Feb 2000 10:06:34 -0800 (PST)
Subject: Re: Desmosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christoph:
I don't have any references at hand, but there is a whole literature on
desmosomes. A search on Medline (or PubMed on the internet) should provide
you with a good list of EM related publications on desmosomes--probably more
than you ever wanted to know! As an aside, I think there may be some books
that cover this as well, but since it is not an area of personal expertise
or current research interest, I am afraid they have all gone from my memory
banks.

Roger


On Tue, 08 Feb 2000 09:56:35 -0800, Christoph Bauer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} we are looking at desmosomes in mouse epidermis. My question: From
} looking at sections we got the impression that demosomes are rather
} uniform, round knob-like structures. As we did not do any serial
} sections, we are not sure if this is true. Does anybody know of a
} publication dealing with this?
}
} Thanks for your help,
}
} Christoph
}
}
}
} Christoph Bauer Ph.D.
} University of Chicago
} Molecular Genentics and Cell Biology
} 5841 S. Maryland Ave/MC 1028
} Chicago, Il 60637
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Wed Feb 09 16:49:20 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 09 Feb 2000 10:06:35 -0800
Subject: Re: Desmosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christoph,
I have examined alot of mouse and pig skin at the TEM level and the
desmosomes and hemidesmosomes appear rather typical, that is, small, long
bridge-like structures between the cells.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky

At 09:56 AM 2/8/00 -0800, Christoph Bauer wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 09 23:51:42 2000



From: Fagerland,Jane :      jane.a.fagerland-at-abbott.com
Date: Wed, 9 Feb 2000 16:45:16 -0600
Subject: Summer internships

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Microscopy and Microanalysis at Abbott Laboratories has two
summer internships available for 8-12 weeks this summer. One position is in
Biological Microscopy, and one is in Materials Analysis and Microscopy.
We're looking for students who are considering a career in microscopy,
especially students interested in the pharmaceutical industry.

Abbott Laboratories is a diversified healthcare company that produces
pharmaceuticals, diagnostic devices, and hospital products. The Microscopy
department analyzes samples related to all these functions. We use polarized
light, fluorescence, TEM, SEM, EDXS, confocal microscopy, and flow cytometry
to solve problems related to products, as well as to provide support for
pharmaceutical Discovery and Development basic research and drug safety.

Housing and a stipend are provided for the summer. We're located near Lake
Michigan and the Wisconsin state border, about an hour's drive or train-ride
north of Chicago. There's lots to see and do in the area, and there are
planned activities with other interns, as well. The Abbott Summer Internship
Program has been nationally recognized as one of the best in the country.

If you're interested, please send a resume to:

Jane A. Fagerland, Ph.D.
Abbott Laboratories
D-45M/AP31
200 Abbott Park Rd.
Abbott Park IL 60064-6202

You may also fax a resume to me at (847) 938-5027 or e-mail it to me at
jane.a.fagerland-at-abbott.com.

If you'd like further information, I can be reached by telephone at (847)
935-0104, or by e-mail. I prefer e-mail, but will be more than happy to talk
to discuss our projects and laboratory by telephone.



From daemon Wed Feb 09 23:52:01 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 9 Feb 2000 20:31:58 -0500
Subject: RE: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is an easy way to determine the milling damage between the two
machines and the amount of ion milling damage (subjectively); compare them
with samples prepared using the small angle cleavage technique. Look at
John McCaffrey's paper on using the small angle cleavage technique in
Ultramicrotomy 38, (149) 1991. He shows the difference between high angle
milling, low angle milling and the small angle cleavage technique. Of
course, the small angle cleavage technique showed no ion milling damage. It
is perfect for these types of materials. If you don't need a site specific
technique, it is the way to go for semiconductors.

You should also look at his paper in the MRS TEM Sample Prep series III (vol
254) that also shows a comparison for a SiGe/Si layer structure. We have a
detailed pictorial outline with tips and tricks on how to do it in the MRS
TEM Sample Prep series IV (vol 480).

I know that you invested a lot in your ion mill, but you can do these
samples very cheaply. SouthBay Technology sells a Microcleave kit that gets
you started with the technique.



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--




} -----Original Message-----
} From: Massimo Catalano [mailto:massimo.catalano-at-ime.le.cnr.it]
} Sent: Wednesday, February 09, 2000 3:43 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: PIPS and milling damage
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear Listservers,
}
} we are massively working on analytical and structural TEM
} characterization
} of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.
}
} Specimen preparation is of course a crucial point of our work.
}
} We have just switched to PIPS (we used to make our samples
} using the Gatan
} Duo Mill), and we are getting controversial results about the damage
} introduced by the milling procedure.
}
} We are perfectly aware of all the differences between the two
} instruments,
} especially the absence of cooling stage and the higher beam current.
}
} Has anyone performed a systematic and careful analysis to try
} to evaluate
} the milling damage on similar materials systems. We are
} willing to start a
} systematic work to assess this issue, but would like to know
} if anyone has
} already done anything on this topic. Also, any collaboration
} will be more
} than welcome.
}
} Thanks.
}
} Massimo
}
}
}
}
} Dr. Massimo Catalano
} CNR-IME
} Campus Universitario
} Via Arnesano
} 73100 Lecce - ITALY
} tel: + 39 0832 322362
} fax: + 39 0832 325299
} email: massimo.catalano-at-ime.le.cnr.it
} http://www.ime.le.cnr.it
} http://www.ime.le.cnr.it/sime/sime.htm
}
}
}



From daemon Wed Feb 09 23:52:03 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 09 Feb 2000 20:34:34 -0500
Subject: Re: LM: Course reminder - DATES...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi again,

It has been brought to my attention that no dates were attached to this
course reminder:

March 10-12, 2000
Hyatt Regency
New Orleans, LA.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



At 07:57 PM 2/7/00 -0500, Barbara Foster wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 09 23:52:05 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 09 Feb 2000 20:39:10 -0500
Subject: Re: tacky wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Linda,

Suggest you contact Chuck Garber at Structure Probe: www.2spi.com
I'm sure that he has some sort of derivative of his tacky dots which would
be helpful


At 08:48 PM 8/27/70 +0000, Linda Chicoine wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 09 23:52:23 2000



From: Christina bennett :      chbennet-at-nmsu.edu
Date: Wed, 9 Feb 2000 21:58:18 -0700
Subject: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know this is off topic however a change like this could make this type of
forum impossible.





} Date: Tue, 8 Feb 2000 08:13:53 -0700
} X-Sender: liperez-at-cnmailsvr.nmsu.edu
} Mime-Version: 1.0
} To: all-at-biology.nmsu.edu
} From: liperez-at-NMSU.Edu (LSPSaldana)
} Subject: Congress to allow email charges
} Sender: owner-all-at-biology.nmsu.edu
} Precedence: bulk
} X-Keywords:
} X-UID: 43
} Status: O
}
}
} } } Congress to allow email charges
} } }
} } } Please pass this on to all you know since many of us use e-mail for
} } } business and to keep up with friends and family, I thought you'd like
} } } to know the following.
} } }
} } } Please jump on it right away and forward this to others.
} } }
} } } CNN has reported that within the next two weeks Congress is going to
} } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } internet access.
} } } Translation: Every time we send long distance e-mail we will receive a
} } } long distance charge. This will get costly. Please visit the following web
} } } site and file a complaint. Complain to your Congressperson. We can't
} } } allow this to pass. The following address will allow you to send an
} } } e-mail on this subject DIRECTLY to your Congressperson.
} } }
} } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } }
} } } Pass this on to your friends. It is urgent! I hope all of you will pass
} } } this on to all your friends and family. We should ALL have
} } } an interest in this one.
} } }
} } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } alarming trend in the Government of the United States attempting to
} } } quietly push through legislation that will affect your use of the
} } Internet.
} } } Under
} } } proposed legislation the U.S. Postal Service will be attempting to bill
} } } email users out of "alternate postage fees". Bill 602P will permit the
} } } Federal Govt. to charge a 5 cent surcharge on every email delivered, by
} } } billing Internet Service Providers at source. The consumer would then be
} } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } working without pay to prevent this legislation from becoming law. The
} } U.S.
} } } Postal Service is claiming that lost revenue due to the proliferation of
} } } email
} } } is costing nearly $230,000,000 in revenue per year. You may have noticed
} } } their recent ad campaign "There is nothing like a letter".
} } } Since the average citizen received about 10 pieces of email per day in
} } } 1998,
} } } the cost to the typical individual would be an additional 50 cents per
} } } day, or over $180 dollars Per year, above and beyond there regular
} } Internet
} } } costs.
} } } Note that this would be money paid directly to the U.S. Postal Service
} } } for a service they do not even provide. The whole point of the Internet is
} } } democracy and non-interference. If the federal government is permitted
} } } to tamper with our liberties by adding a surcharge to email, who knows
} } Where
} } } it will end. You are already paying an exorbitant price for snail mail
} } } because of bureaucratic inefficiency. It currently takes up to 6 days for
} } a
} } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } Service
} } } is
} } } allowed to tinker with email; it will mark the end of the "free"
} } } Internet in the United States.
} } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } dollar per month surcharge on all internet service" above and beyond the
} } } government's proposed email Charges. Note that most of the major
} } } newspapers have ignored the story, the only exception being the
} } } Washingtonian
} } } which called the idea of email surcharge "a useful concept who's time has
} } } come"
} } } (March 6, 1999) Editorial.
} } }
} } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } EVERYONE on your list, and tell all your friends and relatives to write
} } } to their congressman and say "No!" to Bill 602P.
} } }
} } }
} } } It will only take a few moments of your time, and could very well be
} } } instrumental in killing a bill we don't want.
} } }
} } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } NOW, NOT AFTER
} } }
} } }
} } }
} } }
} } }
} } }
} }
}




From daemon Thu Feb 10 19:03:06 2000



From: S.A.Gusev :      gusev-at-ipm.sci-nnov.ru
Date: Thu, 10 Feb 2000 10:51:57 +0300
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Donald,
the Institute of Applied Physics
(http://www.sandy.ru/science/science/appl.new/frames.html)
constructs, produces and uses solid state high energy electron gun for
the High-power electronics and plasma physics researches.
May be they will help you.

Best regards,
Dr. S.A.Gusev


********************************************
* Institute for Physics of Microstrutures *
* Russian Academy of Science *
* ( IPM RAS ) *
* *
* Niznii Novgorod, GSP-105 *
* 603600 *
* RUSSIA *
* *
********************************************

tel: (+7)-8312-675313
fax: (+7)-8312-675553
e-mail: gusev-at-ipm.sci-nnov.ru




From daemon Thu Feb 10 19:03:12 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Thu, 10 Feb 2000 11:29:38 +0200 (EET)
Subject: about my water cooling system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We have got a JEM3010 Transmission electron microscopy.I have got a
one problem about water cooling system.I dont
know,Which kind of water to used?I think so, We should use pure water for
water cooling?Besides My city water is not clean.What do you think about
this problem?
Thanks for your interest

**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************








From daemon Thu Feb 10 19:03:14 2000



From: Matt L Walker :      bmsmlw-at-bms.leeds.ac.uk
Date: Thu, 10 Feb 2000 10:08:40 -0000
Subject: TEM: Re Sarcomere cross-sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am looking for TEM images that show cross-sectional views of skeletal
muscle
sarcomeres during the *contracted state*. In particular, I am
interested in
seeing the spacing of the thin filaments when they **overlap each
other** (i.e.,
when those from one side of the sarcomere overlap those from the other
side of
the sarcomere in the contracted state). In addition, I am interested in
seeing
the distribution of the thin filaments as they pass through the M line.
It
would be greatly appreciated if anyone could tell me if they know of any
research papers or review articles that contain such TEM images.

Thank you!

Izak Paul
Biological Sciences
Mount Royal College

The following papers are classics and would make good starting point.
Huxley, H.E. & Hanson, J. (1954). Nature, 173, 973-976.
Huxley, A.F. & Niedergerke, R.M. (1954). Nature, 173, 971.
Huxley, H.E. (1957). Biophys. & Biochem. Cytol. 3, 631-648.

Thin filaments 'pass through' the Z-line. Try John Squire and Pradeep
Luther
(3D reconstruction of fish Z-disc) and Mike Reedy (insect) or Bellinda
Bullard.

Matt Walker
School of Biomedical Sciences
Leeds University, UK





From daemon Thu Feb 10 19:03:25 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 10 Feb 2000 11:26:59 +0000
Subject: Film scanners - that old chestnut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm sorry to bring this topic up yet again, but I have just come across
the specification for a flat-bed scanner which looks as if it would be
suitable for scanning e.m. cut-film. It is the Epson Perfection 1200
Photo which includes a 5x4inch film adapter.

The UK web site address is:
http://www.epson.co.uk/sohoprod/imaging/scanner/perf1200/perf1200photo/spec.htm

and the specification is:
A4 Colour flatbed with 5"x4" film image scanner Single pass scanning
Optical resolution: 1200 x 2400 (30600 pixels/line)
Maximum output resolution of 9600x9600 dpi
36 bits per pixel in colour (24 bit output)
12 bits per pixel in black (8 bit output)
Optical Density 3.2D
USB (Type B)

Does anyone have any experience of this machine because it is about a
fifth of the price of the Umax flatbed film scanners? My only
reservation is that it is a SOHO product rather than professional.


Thanks in advance

Malcolm

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk



From daemon Thu Feb 10 19:04:04 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 10 Feb 2000 05:34:15 -0600
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a recurring post about nothing more than a hoax.

Search for internet hoaxes and so forth and you will find
this one and many other interesting yet equally invalid assertions.

gary g.


At 10:58 PM 2/9/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Feb 10 19:03:44 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Thu, 10 Feb 2000 13:38:12 +0100
Subject: coherence and spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The statement that the smallest spot size gives the best coherence can be a
misleading. The full statement should be the spot size with the smallest
angular distribution should be used. Anyone who has tried to do off-axis
holography in nanoprobe mode will testify to this.
The spatial coherence envelope, roughly the distance over which the beam is
considered 'coherent', is the Fourier transform of the angular distribution
of intensity at the source (Van Cittert Vernike theorem).

Born & Wolf (section 10.4.2)
"Hence if the linear dimensions of the source and the distance between P1
and P2 (points in the imaging plane) are small compared to the distance of
these points from the source, the degree of coherence, |mu_12| is equal to
the absolute value of the normalized Fourier transform of the intensity
function of the source"

The reason holography and high resolution work is done with te most
parallel beam possible becomes clear, the angular distribution of a
converged (or diverged beam) is wide enough to reduce the spatial coherence
envelope. In off-axis holography this is even more stringent since two
interfering points (reference wave & object wave) can be hundreds of
nanometers, microns even, apart. Elliptical illumination is used to
preserve coherence in the one important direction (minimum angular width)
and converged in the other to improve the intensity.


********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Thu Feb 10 19:03:46 2000



From: EvexAnalyt-at-aol.com
Date: Thu, 10 Feb 2000 07:49:14 EST
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christine,

I read the following just the other day, from the US Postal Service

http://www.usps.gov/news/press/99/99045new.htm


FOR IMMEDIATE RELEASE
May 21, 1999
Release No. 45

E-MAIL RUMOR COMPLETELY UNTRUE

WASHINGTON – A completely false rumor concerning the U.S. Postal Service is
being circulated on Internet e-mail. As a matter of fact, the Postal Service
has learned that a similar hoax occurred recently in Canada concerning Canada
Post.

The e-mail message claims that a "Congressman Schnell" has introduced "Bill
602P" to allow the federal government to impose a 5-cent surcharge on each
e-mail message delivered over the Internet. The money would be collected by
Internet Service Providers and then turned over to the Postal Service.

No such proposed legislation exists. In fact, no "Congressman Schnell" exists.

The U.S. Postal Service has no authority to surcharge e-mail messages sent
over the Internet, nor would it support such legislation.

-30-


Evex Analytical
X-ray Analyzers and Digital Imaging Systems
857 StateRoad
Princeton, NJ 08540
609-252-9192 T
609-252-9091 F
www.evex.com
info-at-evex.com




In a message dated 2/10/00 12:26:56 AM Eastern Standard Time,
chbennet-at-nmsu.edu writes:

{ { Subj: FYI: Congress to allow email charges
Date: 2/10/00 12:26:56 AM Eastern Standard Time
From: chbennet-at-nmsu.edu (Christina bennett)
To: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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I know this is off topic however a change like this could make this type of
forum impossible.





} Date: Tue, 8 Feb 2000 08:13:53 -0700
} X-Sender: liperez-at-cnmailsvr.nmsu.edu
} Mime-Version: 1.0
} To: all-at-biology.nmsu.edu
} From: liperez-at-NMSU.Edu (LSPSaldana)
} Subject: Congress to allow email charges
} Sender: owner-all-at-biology.nmsu.edu
} Precedence: bulk
} X-Keywords:
} X-UID: 43
} Status: O
}
}
} } } Congress to allow email charges
} } }
} } } Please pass this on to all you know since many of us use e-mail for
} } } business and to keep up with friends and family, I thought you'd like
} } } to know the following.
} } }
} } } Please jump on it right away and forward this to others.
} } }
} } } CNN has reported that within the next two weeks Congress is going to
} } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } internet access.
} } } Translation: Every time we send long distance e-mail we will receive a
} } } long distance charge. This will get costly. Please visit the following
web
} } } site and file a complaint. Complain to your Congressperson. We can't
} } } allow this to pass. The following address will allow you to send an
} } } e-mail on this subject DIRECTLY to your Congressperson.
} } }
} } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } }
} } } Pass this on to your friends. It is urgent! I hope all of you will pass
} } } this on to all your friends and family. We should ALL have
} } } an interest in this one.
} } }
} } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } alarming trend in the Government of the United States attempting to
} } } quietly push through legislation that will affect your use of the
} } Internet.
} } } Under
} } } proposed legislation the U.S. Postal Service will be attempting to bill
} } } email users out of "alternate postage fees". Bill 602P will permit the
} } } Federal Govt. to charge a 5 cent surcharge on every email delivered, by
} } } billing Internet Service Providers at source. The consumer would then be
} } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } working without pay to prevent this legislation from becoming law. The
} } U.S.
} } } Postal Service is claiming that lost revenue due to the proliferation of
} } } email
} } } is costing nearly $230,000,000 in revenue per year. You may have noticed
} } } their recent ad campaign "There is nothing like a letter".
} } } Since the average citizen received about 10 pieces of email per day in
} } } 1998,
} } } the cost to the typical individual would be an additional 50 cents per
} } } day, or over $180 dollars Per year, above and beyond there regular
} } Internet
} } } costs.
} } } Note that this would be money paid directly to the U.S. Postal Service
} } } for a service they do not even provide. The whole point of the Internet
is
} } } democracy and non-interference. If the federal government is permitted
} } } to tamper with our liberties by adding a surcharge to email, who knows
} } Where
} } } it will end. You are already paying an exorbitant price for snail mail
} } } because of bureaucratic inefficiency. It currently takes up to 6 days for
} } a
} } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } Service
} } } is
} } } allowed to tinker with email; it will mark the end of the "free"
} } } Internet in the United States.
} } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } dollar per month surcharge on all internet service" above and beyond the
} } } government's proposed email Charges. Note that most of the major
} } } newspapers have ignored the story, the only exception being the
} } } Washingtonian
} } } which called the idea of email surcharge "a useful concept who's time has
} } } come"
} } } (March 6, 1999) Editorial.
} } }
} } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } EVERYONE on your list, and tell all your friends and relatives to write
} } } to their congressman and say "No!" to Bill 602P.
} } }
} } }
} } } It will only take a few moments of your time, and could very well be
} } } instrumental in killing a bill we don't want.
} } }
} } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } NOW, NOT AFTER
} } }
} } }
} } }
} } }
} } }
} } }
} }
}





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Date: Wed, 9 Feb 2000 21:58:18 -0700
To: microscopy-at-sparc5.microscopy.com
From: Christina bennett {chbennet-at-nmsu.edu}
Subject: FYI: Congress to allow email charges
Errors-to: Microscopy-request-at-sparc5.Microscopy.Com

} }



From daemon Thu Feb 10 19:03:37 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 10 Feb 2000 07:14:00 -0600
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is this yet another hoax eating time & bandwidth by being posted without
confirmation (like the last 3-4 times I saw this message in the pas year or
two), or is it real?

I could find no reference to it at the FCC site.... Those people who are
REALLY
in charge of telecommunication regulations....

Woody

New format, more pix:
http://www.geocities.com/capecanaveral/3722



From daemon Thu Feb 10 19:03:38 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 10 Feb 2000 07:29:00 -0600
Subject: Noise vs. Be

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Harry,

Weelll... For most systems, if the low end noise is not inhibited (most
are),
the noise generated, apparent x-ray intensity, tends to increase with lower
ev.
This would not produce a gaussian-like peak, but a monotonic rise in
intensity
with lower ev. OTOH, pulse processor circuitry *could* be designed/setup to
produce such an effect. Seeing Be x-rays from 100% element is difficult to
impossible with most EDS systems. If compounded, the odds get worse.

Woody



From daemon Thu Feb 10 19:03:53 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Thu, 10 Feb 2000 08:29:03 -0500
Subject: Re: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the early 1980's Philips believed that their research labs had
successfully developed a new electron source suitable for electron
microscopes. Indeed there was a time when they were planning to sell
microscopes with the new sources. I never heard what went wrong. There
were rumors that the lifetime was not good enough.

Anyone who wants details can find them in their library:
"An Efficient Silicon Cold Cathode for High Current Densities"
Van Gorkom and Hoeberechts
Philips Journal of Research 39 (1984) 51-60


Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvannia 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu



From daemon Thu Feb 10 19:03:49 2000



From: David_Bell-at-Millipore.com
Date: Thu, 10 Feb 2000 08:29:28 -0500
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi all,

This, of course, is a classic internet hoax. I refer you to the following
website:


http://ciac.llnl.gov/ciac/CIACHoaxes.html#internetcharge

This hoax is designed, like many of the others, to eat up bandwidth and get
people involved in a general uproar.

Hopefully, no one panicked here! It may be worth the time to bookmark this hoax
site, or a similar one, so that when
we get messages such as this in our email, we can check their validity, before
contributing to its spread.
No insult intended towards anyone who falls victim to these emails, as I'm sure
most of us have at one time
or another.

Sincerely,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Thu Feb 10 19:04:09 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Thu, 10 Feb 2000 08:54:18 -0500
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is one of those internet hoaxes. It is not true, do not send it to
your friends, do not write your congressperson. Mining Co has a great web
page that debunks these hoaxes and myths
{http://urbanlegends.miningco.com/culture/urbanlegends/library/blhoax.htm?pid=27
33&cob=home} and is a good place to check before sending on any email that
urges you to send it to everyone you know. This message is a combination
of two old and popular hoaxes, see
{http://urbanlegends.miningco.com/culture/urbanlegends/library/blemtax2.htm}
and
{http://urbanlegends.about.com/culture/urbanlegends/library/weekly/aa012099.htm}
Hopefully this will die here.
Scott


} } } } Congress to allow email charges
} } } }
} } } } Please pass this on to all you know since many of us use e-mail for
} } } } business and to keep up with friends and family, I thought you'd like
} } } } to know the following.
} } } }
} } } } Please jump on it right away and forward this to others.
} } } }
} } } } CNN has reported that within the next two weeks Congress is going to
} } } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } } internet access.
} } } } Translation: Every time we send long distance e-mail we will receive a
} } } } long distance charge. This will get costly. Please visit the following web
} } } } site and file a complaint. Complain to your Congressperson. We can't
} } } } allow this to pass. The following address will allow you to send an
} } } } e-mail on this subject DIRECTLY to your Congressperson.
} } } }
} } } } http://www.house.gov/writerep {http://www.house.gov/writerep}

..sniped...
} } } }
} } } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } } NOW, NOT AFTER


-------------------note: new mailing address----------------------
Scott Wight e-mail: scott.wight-at-nist.gov
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | fax: 301-417-1321
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Thu Feb 10 19:04:50 2000



From: William Janssen :      bjanssen-at-neuro.mssm.edu
Date: Thu, 10 Feb 2000 10:31:03 -0400
Subject: position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A technical position is available in the Neurobiology of Aging program at the
Mount Sinai School of Medicine in New York. We are seeking an experienced
Electron Microscopist with a BS/BA or MS in Biology/Life Sciences. Applicants
should have excellent communication and organizational skills, an understanding
of basic laboratory procedures, and the ability to manage a large and varied
workload. The successful candidate will participate in ultrastructural studies
focusing on the effects of: estrogen and aging on hippocampal circuitry
which is implicated in learning and memory, quantitative excitatory amino
acid receptor (NMDA and AMPA) distribution within the central nervous
system of transgenic models, as well as manipulated primate and rodent
models. Qualifications include at least 2 years of experience in routine
transmission electron microscopy procedures,
ultramicrotomy, immunogold labelling, specimen preparation, digital
photography, and routine maintenance of equipment. Experience with
immunofluorescence and confocal microscopy is an asset.

We offer a salary commensurate with experience and excellent benefits. For
consideration, please mail/email your resume to:

Bill Janssen
Neurobiology of Aging
Box 867, EB9-02
Mount Sinai School of Medicine,
One Gustave L. Levy Place,
New York, NY 10029-6574

We are an equal opportunity employer fostering diversity in the workplace.
Bill Janssen
Neurobiology of Aging Laboratories
Mount Sinai School of Medicine, Box 1639
One Gustave L. Levy Place
New York, NY 10029

Phone 212-824-8789
Fax 212-849-2510



From daemon Thu Feb 10 19:04:37 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Thu, 10 Feb 2000 14:55:24 +0000
Subject: Re: about my water cooling system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I think the best solution would be to use a water recirculatory
system. Fill it with clean water and it should stay clean for a
long time. We use a 'Neslab'

Hope this helps

Alan Walker.

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://www.shef.ac.uk/uni/academic/D-H/eee/
*********************************************



From daemon Thu Feb 10 19:04:48 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 10 Feb 2000 07:59:28 -0700
Subject: Re: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rosemary,

this is of course a recurring theme in image analysis. There really is
no easy answer to this. Counting particles is easier as you can perhaps
separate the particles and arrive at the correct count, but if you don't
know what is occluded, you cannot measure it. However, if you can use
some information that is available to make some guesses or
extrapolations what the shape is, it can be done. A simple example: If
you look at a heap of coins, they may overlap. It is nevertheless
possible to measure the individual coins because I know that they are
all round. So I can take the "protruding" part of a coin and simply fit
a circle and that should give me the size pretty accurately.

Are ice crystals like snowflakes? Snowflakes, if I remember correctly,
have a six-fold symmetry. Can't you just measure one "branch" of a
snowflake (the one that you can see), and multiply that by 6? I am not
an expert on ice crystals (although I love to ski), so this may be
oversimplified. You may have to think about all the information you have
about ice crystals and can then perhaps make some guesses about the
occluded part.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From: Rosemary Walsh[SMTP:RW9-at-PSU.EDU]
} Sent: Tuesday, February 08, 2000 3:24:35 PM
} To: microscopy-at-sparc5.Microscopy.Com
} Subject: Size determination of overlapping particles
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Thu Feb 10 19:04:13 2000



From: spatel-at-goodyear.com
Date: Thu, 10 Feb 2000 09:59:36 -0500
Subject: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Subscribers;

We have a tedious task of liquid nitrogen transfer and fill-up on our
instruments such as SEMs, TEM and for cryo-ultramicrotomes. Is there any
compact unit on market which would condense nitrogen from air into liquid
form? I know that Liquid oxygen would be a problem. TIA;

Siddharth Patel



From daemon Thu Feb 10 19:04:51 2000



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Thu, 10 Feb 2000 10:10:27 -0500
Subject: Re: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Massimo,

I just read the two excellent replies from Scott and Phillip and fully agree
with them. Small angle cleavage technique (SACT) and the tripod polisher are
two very useful TEM prep techniques. In my experience, SACT is easier to learn
than tripod polishing but tripodding is applicable to more materials and yields
larger thin areas than does SACT.

As Phillip mentions, low energy milling in the final step of the process is
extremely important for reducing artifacts. I would finish using the lowest
possible energy that still yields effective milling.

Another approach is to use reactive ion beam etching (RIBE) or chemically
assisted ion beam etching (CAIBE). In both these techniques a reactive gas
(usually Iodine) is used. In RIBE, iodine is actually ionized and is used as
the milling gas. In CAIBE, iodine gas is introduced into the milling chamber
directly adjacent to the sample during argon ion milling. Both these
techniques have been shown to eliminate the ion beam damage for binary type
III-V compound semiconductors containing InP. Chew and Cullis (Ultramicroscopy
23/1987/175-198) also report improvements of ion milled surfaces for ternary
type III-V semiconductors containing InGaAs (materials you mentioned) using
RIBE.

I believe that Gatan offers an optional CAIBE attachment for the PIPS. This
may be the easiest thing to try first. Remember that iodine is very corrosive
and can damage ion milling parts if not used properly. Check with Gatan for
their recommendations.

Hope this helps,

Eric W.

Eric S. Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
Fax: (301) 417-1321
Eric.Windsor-at-NIST.Gov


On Feb 9 -at- 09:43 Massimo Catalano wrote:

Dear Listservers,
we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.
Specimen preparation is of course a crucial point of our work.
We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.
We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.
Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.
Thanks.
Massimo



Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm



Eric S. Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
Fax: (301) 417-1321
Eric.Windsor-at-NIST.Gov



From daemon Thu Feb 10 19:04:42 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 10 Feb 2000 15:12:21 +0000
Subject: Re: Film scanners - that old chestnut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Maureen and anyone else who's interested

I have found the basic description on the US/Canada website so I assume
its available there too - see address below:
http://www.epson.com/cam_scan/scanners/perfection1200/index.html

The price inclusive of transparency adapter appears to be about 200 UK
pounds over here. There seems to be three models:
1200s (standard print scanner - SCSI interface)
1200u (standard print scanner - USB interface)
1200photo (standard print scanner + 5x4inch transparency adapter - USB
interface)
It's the last of these that is of interest.

Malcolm Haswell
------------------------------------------
Maureen Petersen wrote:
}
} Malcolm:
}
} Will you divulge what this gem costs? Do you know if it is available in the
} US?
}
} Thank you, Maureen Petersen
} Dept Plant Pathology
} University of Florida
} Gainensville, FL 32611
}
} I'm sorry to bring this topic up yet again, but I have just come across
} the specification for a flat-bed scanner which looks as if it would be
} suitable for scanning e.m. cut-film. It is the Epson Perfection 1200
} Photo which includes a 5x4inch film adapter.
}
} The UK web site address is:
} http://www.epson.co.uk/sohoprod/imaging/scanner/perf1200/perf1200photo/spec.
} htm
}
} and the specification is:
} A4 Colour flatbed with 5"x4" film image scanner Single pass scanning
} Optical resolution: 1200 x 2400 (30600 pixels/line)
} Maximum output resolution of 9600x9600 dpi
} 36 bits per pixel in colour (24 bit output)
} 12 bits per pixel in black (8 bit output)
} Optical Density 3.2D
} USB (Type B)
}
} Does anyone have any experience of this machine because it is about a
} fifth of the price of the Umax flatbed film scanners? My only
} reservation is that it is a SOHO product rather than professional.
}
} Thanks in advance
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscopy
} School of Sciences
} Fleming Building
} University of Sunderland
} SUNDERLAND SR1 3SD
} Tyne and Wear
} UK
}
} Tel (0191) 515 2872
} e-mail: malcolm.haswell-at-sunderland.ac.uk



From daemon Thu Feb 10 19:06:23 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Thu, 10 Feb 2000 10:23:04 -0500
Subject: Color vs. monochrome cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am considering the purchase of a new digital camera. Some of the new
cameras like the Spot-RT and Magnafire have the option of running as three
shot color or one shot gray-scale acquisition. If someone wants to do dual
channel fluorescence like FITC/Rhod or annexin/PI, we can do that as either
acquiring each image with a separate filter cube and combining in software,
or designing a dual filter cube and acquiring in color (or I guess
acquiring separate color images iwth the single cubes and combining??).
What is people real world experience with the relative merits of these two
approaches. It seems that most of what I read is done by separate filter
cubes combined in software, but there also seems to be a push toward fast
cooled color cameras. The advantage I can see to the color approach would
be using a color chip camera you get multiple channels simultaneously.
However, do you have to expose significantly longer to acheive this
compared to multiple gray scale acquisitions? I can't see an obvious
advantage to the three shot color approach because I suspect the light
throughput is lower and the filters sets I suspect are expensive to add on
if you already have the single fluor filters. The only real advantage I
see to three shot acquisition is the ability to easily image
stained/histology transmitted light slides and not for fluorescence. Any
thoughts appreciated. Thanks- Dave


************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Thu Feb 10 19:04:58 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Thu, 10 Feb 2000 10:38:48 -0500 (EST)
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This nonsense resurfaces every few months. There has
never been any truth to it. (Of course, there's no
assurance that Congress will not act so foolishly in the
future.)

Kal

On Wed, 9 Feb 2000, Christina bennett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I know this is off topic however a change like this could make this type of
} forum impossible.
}
}
}
}
}
} } Date: Tue, 8 Feb 2000 08:13:53 -0700
} } X-Sender: liperez-at-cnmailsvr.nmsu.edu
} } Mime-Version: 1.0
} } To: all-at-biology.nmsu.edu
} } From: liperez-at-NMSU.Edu (LSPSaldana)
} } Subject: Congress to allow email charges
} } Sender: owner-all-at-biology.nmsu.edu
} } Precedence: bulk
} } X-Keywords:
} } X-UID: 43
} } Status: O
} }
} }
} } } } Congress to allow email charges
} } } }
} } } } Please pass this on to all you know since many of us use e-mail for
} } } } business and to keep up with friends and family, I thought you'd like
} } } } to know the following.
} } } }
} } } } Please jump on it right away and forward this to others.
} } } }
} } } } CNN has reported that within the next two weeks Congress is going to
} } } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } } internet access.
} } } } Translation: Every time we send long distance e-mail we will receive a
} } } } long distance charge. This will get costly. Please visit the following web
} } } } site and file a complaint. Complain to your Congressperson. We can't
} } } } allow this to pass. The following address will allow you to send an
} } } } e-mail on this subject DIRECTLY to your Congressperson.
} } } }
} } } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } } }
} } } } Pass this on to your friends. It is urgent! I hope all of you will pass
} } } } this on to all your friends and family. We should ALL have
} } } } an interest in this one.
} } } }
} } } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } } alarming trend in the Government of the United States attempting to
} } } } quietly push through legislation that will affect your use of the
} } } Internet.
} } } } Under
} } } } proposed legislation the U.S. Postal Service will be attempting to bill
} } } } email users out of "alternate postage fees". Bill 602P will permit the
} } } } Federal Govt. to charge a 5 cent surcharge on every email delivered, by
} } } } billing Internet Service Providers at source. The consumer would then be
} } } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } } working without pay to prevent this legislation from becoming law. The
} } } U.S.
} } } } Postal Service is claiming that lost revenue due to the proliferation of
} } } } email
} } } } is costing nearly $230,000,000 in revenue per year. You may have noticed
} } } } their recent ad campaign "There is nothing like a letter".
} } } } Since the average citizen received about 10 pieces of email per day in
} } } } 1998,
} } } } the cost to the typical individual would be an additional 50 cents per
} } } } day, or over $180 dollars Per year, above and beyond there regular
} } } Internet
} } } } costs.
} } } } Note that this would be money paid directly to the U.S. Postal Service
} } } } for a service they do not even provide. The whole point of the Internet is
} } } } democracy and non-interference. If the federal government is permitted
} } } } to tamper with our liberties by adding a surcharge to email, who knows
} } } Where
} } } } it will end. You are already paying an exorbitant price for snail mail
} } } } because of bureaucratic inefficiency. It currently takes up to 6 days for
} } } a
} } } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } } Service
} } } } is
} } } } allowed to tinker with email; it will mark the end of the "free"
} } } } Internet in the United States.
} } } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } } dollar per month surcharge on all internet service" above and beyond the
} } } } government's proposed email Charges. Note that most of the major
} } } } newspapers have ignored the story, the only exception being the
} } } } Washingtonian
} } } } which called the idea of email surcharge "a useful concept who's time has
} } } } come"
} } } } (March 6, 1999) Editorial.
} } } }
} } } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } } EVERYONE on your list, and tell all your friends and relatives to write
} } } } to their congressman and say "No!" to Bill 602P.
} } } }
} } } }
} } } } It will only take a few moments of your time, and could very well be
} } } } instrumental in killing a bill we don't want.
} } } }
} } } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } } NOW, NOT AFTER
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
}
}





From daemon Thu Feb 10 19:05:08 2000



From: David_Bell-at-Millipore.com
Date: Thu, 10 Feb 2000 10:50:30 -0500
Subject: Information on RG Lee SEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Colleagues,

Someone at a remote location within our company has decided to purchase an RG
Lee SEM. I am looking for information and opinions from people who have one of
these microscopes. I'm interested in any service issues, reliability issues and
usability concerns that anyone may have. The model they are interested in is
the PSM 75LS. Also, if anyone has the URL for their website, that would be
appreciated, as well.

Thanks in advance,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Thu Feb 10 19:05:17 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Thu, 10 Feb 2000 17:00:49 +0100
Subject: RE: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Scott,

A poster does not mean anything. I have seen them with the Lithium-Ka energy
listed, although quantum physics does forbid this X-ray line. But I can
assure you the Be-K line does exist.

On modern EDX systems Be can be detected, provided you have either a
windowless detector or a detector with a modern polymer type window (SUTW,
SATW, Norvar, or whatever the EDX supplier calls it. My apologies if I break
any trade-marks here...).
On most demonstration setups your EDS specialist will be pleased to show you
a Be peak. But probably from a pure Be sample only. The absorption
coefficient (MAC) of Be in basically any matrix is so huge, and the X-ray
fluorescence yield so small, that in any compound with less than 50 atomic
percent Be you will not detect a visible Be peak. For this reason most EDX
installations "in the field" are not setup to detect Be-K radiation.

WDS has a better P/B ratio, so if you have the proper multilayer crystal you
can get results. But peak shifts and peak shape alterations will make
quantification extremely difficult, not to mention the fact that for most
MACs of Be-K in any matrix we only have a ball-park figure.

Other techniques, like PEELS, are much more suitable for Li and Be analysis.
Best regards,

Hans Dijkstra

Disclaimer: This is my opinion, and not necessarily the one from EDAX Inc.
-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------


} -----Original Message-----
} From: Walck. Scott D. [mailto:walck-at-ppg.com]
} Sent: Tuesday, February 08, 2000 6:57 PM
} To: 'microprobe'; 'harry.ekstrom-at-honeywell.com'
} Cc: 'Microscopy'
} Subject: RE: Be X-ray peaks -I don't think so
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm wrong. I guess I should have looked up at the X-ray periodic table
} above my head and saw the 0.108 keV value for Be. The reason that I was
} wrong was that I did not consider the solid state aspects. I am still
right
} about not having a 2s1/2 to a 1s1/2 transition and that an X-ray carries
} away 1h-bar of angular momentum. What I didn't do was think about the
band
} structure of a solid. If you have the solid, the 2p bands of Be most be
} spilling into or be the conduction band for the electrons. That must be
the
} source for the electronic transition. I guess I should have engaged brain
} before typing.
} My most humble apologies.
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
}
} "The opinions expressed are those of Scott D. Walck and not of PPG
} Industries, Inc. nor of any PPG-associated companies."
} --
}



From daemon Thu Feb 10 19:05:23 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 10 Feb 2000 08:08:10 -0800
Subject: urban myths and legends - Congress to allow e-mail charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are two URLs showing that this is just another hoax;


http://www.urbanmyths.com/email_internettax602p.html



http://www.ulrc.com.au/html/report.asp?CaseFile=ULRR0027&Page=1&View=Request

gary g.



From daemon Thu Feb 10 19:05:29 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Thu, 10 Feb 2000 11:16:00 -0500
Subject: New England Society for Microscopy-March 8th Meeting Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The first meeting of the millenium for NESM (New England Society for
Microscopy) will be held on
March 8, 2000 at Polaroid Corporation in Wayland, MA. Registration will
begin at 5:00pm with tours
of the (new) facility following at 5:30pm. Attendees will also be asked to
fill outa questionnaire (optional) on "Professional Imaging" at this time.

A buffet supper will be served from 6-7pm followed by three scientific
presentations. The speakers
are : Gabriel Rojano, Staff Reliability Engineer-MIA-Com (Tyco Electronics
Company), Paul Bain from the Harvard Medical Library and Dr. Hjalmar
Brismer from the Karolinska Institute in Stockhold, Sweden.

Advance registration is encouraged (by March 3rd). The registration fee
for members is $5.00 and $20.00 for non-members (this includes a current
one-year membership in the society). Interested parties should contact:
Peggy Sherwood, Corresponding Secretary (MESnesm-at-aol.com).






From daemon Thu Feb 10 19:05:38 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 10 Feb 2000 08:29:18 -0800
Subject: Re: Congress to allow e-mail charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Different subject title in case other posting got filtered out.

} Date: Thu, 10 Feb 2000 08:08:10 -0800
} To: Microscopy-at-MSA.Microscopy.Com
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
} Subject: urban myths and legends - Congress to allow e-mail charges
}
} Here are two URLs showing that this is just another hoax;
}
}
} http://www.urbanmyths.com/email_internettax602p.html
}
}
}
} http://www.ulrc.com.au/html/report.asp?CaseFile=ULRR0027&Page=1&View=Request
}
} gary g.



From daemon Thu Feb 10 19:05:43 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Thu, 10 Feb 2000 11:48:39 -0500 (EST)
Subject: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The insidious nature of this hoax/scam/... is that not only does the
original hoax get circulated, using up bandwidth, but then the replies of
the people who identified the hoax, many with the full hoax message
attached, also get circulated. And then the triple play (baseball and spring
training is near) is when people like me comment on the aforesaid. Please,
please don't reply to this note (which should have no attachments!) and make
it a home run!

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Thu Feb 10 19:05:45 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Thu, 10 Feb 2000 09:00:52 -0800
Subject: RE: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is not true. It is an internet hoax. Here's the info from an urban
legends website:
---------------------------
Claim: Congress will soon be voting on whether or not your phone company
will be
allowed to charge you long-distance rates for accessing the Internet.

Status: False.

Example: [Collected on the Internet, 1999]

Please forward this to everyone you can...

There is a new bill in the US Congress that will affect ALL INTERNET
USERS. CNN stated that the Government would in two weeks time
decide whether to allow or not allow a Charge to YOUR phone bill equal
to a long distance call each time you access the Internet.

This affects us all! We cannot allow this to happen! Please visit the
following URL and fill out the necessary form to let your Congressman
know how you feel! The address is http://www.house.gov/writerep/.
Write
your representative!

Synopsis: Neither the FCC nor Congress is considering -- much less
voting on --
legislation that would impose (or allow phone companies to impose)
per-minute access fees
on Internet users. Recent decisions by the FCC in this area have dealt
only with the issue of
how phone companies reimburse each other for handling calls placed to
Internet service
providers, not with the prospect of allowing phone companies to charge
their customers
long-distance rates for Internet use.

Origins: As soon as we get hold of something we really like at a
reasonable price, we start
worrying that it will be banned, taxed, or made too expensive for us to
afford. The Internet is
no exception, as our old friend -- the capitalized, exclamation pointed,
"send this to everyone
you know" anonymous e-mail message -- is here to tell you.

Way back when in 1987, the Federal Communications Commission did
consider imposing a
surcharge for transmitting data over the public telephone network, but
they ultimately
rejected the idea (thanks in part to the more than 10,000 letters of
complaint they received).
Unable to believe our good fortune (the government wasn't going to make
us pay through
the nose for dialing up all those neat computer bulletin boards we'd
discovered), we couldn't
leave well enough alone, and in 1991 a flood of urgent messages warning
us that the FCC
was again considering a proposal they'd rejected three years earlier hit
e-mail systems all
over the country (and the nascently popular Internet). Like the
ubiquitous Craig Shergold
message, the "modem tax" warning would long outlive the validity of the
information it
conveyed.

Fast forward to 1998. On-line services, the Internet, the World Wide
Web, e-mail, and chat
rooms are more popular than ever, a daily part of many people's lives.
Somebody -- the
government, the phone companies, Bill Gates, the Grinch -- must be on
his way to spoil the
party. Sure enough, we're now being told the phone companies and the
government are in
cahoots to ruin our good time.

First of all, a little background. Most of us still have to dial up over
a modem and connect to
an Internet Service Provider (ISP) to access the Internet. If your ISP
is in your local dialing
area, you probably don't pay anything at all for the call, no matter how
long you stay
connected. This means you get to tie up a phone line with your modem for
hours and hours
on end, every day, at no charge beyond the price of basic phone service.
And the party at the
other end of the line -- your ISP -- isn't paying anything extra,
either. It's easy to see that
somebody has a chance to reap some windfall profits here. If the phone
companies were
allowed to charge you a per-minute fee for accessing the Internet (or
the government were
allowed to tax your use of the Internet), their coffers would soon
overflow with cash.

Scary thought, isn't it? All the phone companies need, we hear, is to
get the FCC to
reclassify and/or regulate ISPs, and then the phone companies can charge
gobs of extra
money for handling Internet traffic. And Congress is just about to vote
on that very issue,
we're told.

In fact, there is no such proposal before Congress, and there never has
been.

The only real issue before the FCC concerning Internet usage (and the
genesis of this latest
round of scaremongering) is the subject of "reciprocal compensation." In
short, reciprocal
compensation means that when you place a local call to someone who is
serviced by a
different phone company, your phone company has to compensate his phone
company for
completing the call. (On the other hand, when you place a long-distance
call, the long
distance carrier who handles the traffic has to pay access charges to
your phone company
for originating the call.) But if the "person" you're calling is an ISP,
should your phone
company have to compensate the ISP's phone company?

The subject of recpirocal compensation has been a hot issue of late
because new local phone
companies have been springing up all over the place. The bigger phone
companies, figuring
that they would have many more customers than their newer (and smaller)
competitors,
negotiated reciprocal compensation agreements with the new phone
companies. Every time
one of these little phone companies' customers placed a call to a
destination outside his local
service that ended up on the bigger phone company's network, the big
phone company
would get to collect money from the little phone company. Not a bad
scheme, the big phone
companies thought.

Ah, but some of the little guys had a neat trick up their sleeves. They
started offering their
services to Internet service providers -- ISPs with banks and banks of
modems that received
thousands and thousands of calls every day, but never made any outgoing
calls. All the
reciprocal compensation started flowing one direction, from the big
phone companies to the
little phone companies, which wasn't what the big guys had in mind at
all. "Foul," they cried.
"Internet traffic flows all over the world," they noted. "Internet
traffic is therefore interstate in
nature and should be classified as long-distance, so the little guys
should be paying us for
originating the calls," they insisted. "We're not paying," they sputtered.

Enter the FCC to resolve the dispute, which they did (for now) on 25
February 1999 by
ruling that phone companies are bound by whatever reciprocal
compensation agreements
they've negotiated with each other, whether they think they're fair or
not. That was the only
issue before the FCC. But most of us are already struggling with a glut
of information, and
we don't have the time to familiarize ourselves with details like
interconnection agreements
and reciprocal compensation, so when we hear reports with buzzwords like
"Internet,"
"FCC," "access fees," and "long distance," we assume the worst, even
though the real story
is something quite different. And even if we make the effort to
understand the whole story,
we find all too often that we're reading information that has been
misreported by others --
often the mainstream media -- who didn't make enough of an effort to
understand the whole
story themselves. If we can't even depend upon the people whose business
it is to supply us
with accurate information to get the facts straight, what more can we
do? (See, for example,
the misleading headline on the CNN article referenced in the "Additional
information"
section below.)

A few important points related to the recent FCC decision:

Didn't the FCC rule that Internet connections are long-distance
calls?

Sort of. The FCC declared that "Internet traffic is
jurisdictionally mixed and appears to
be largely interstate in nature," which is the technical
definition of "long distance." But
that doesn't mean -- as is often misreported -- that Internet
users will be paying long
distance rates for dial-up connections, since the Internet has
been, and still is, exempt
from interstate access charges. The FCC did nothing to abolish
that exemption.

Won't the phone companies just pass the cost of carrying Internet
traffic to
customers by raising their phone rates?

There is no guarantee that phone rates won't go up in the future,
of course. However,
since most states require phone companies to charge a flat rate
for unlimited local
usage, you won't have to pay per-minute charges for accessing the
Internet (as long as
your ISP has a dial-up number within your local service area).

What if the FCC changes their mind?

The possibility exists that the FCC might someday decide that
additional fees can be
imposed for Internet access. But as Bill Kennard, the chairman of
the FCC, has stated
on more than one occasion: "I want to say this as clearly as I can
. . . as long as I'm
chairman of the Federal Communications Commission this agency will
not regulate
the Internet. It's not going to happen. The FCC has no intention
of making computer
users pay long distance fees for dial-up access to the Internet,
as people now pay when
they make long-distance telephone calls. These rumors get on the
Internet that the big
bad FCC is going to impose all this regulation on the Internet.
Now I know this
painfully because every so often when one of these rumors flares
up I get, literally,
about 600 e-mail messages a day by people who are telling me to
keep my hands off
the Internet."
[Note: this is not a direct quote from Kennard; it is pieced
together from multiple
statements of his.]

Additional information:

No Consumer Per-Minute Charges to Access ISPs (FCC)

Users, Advertisers Await FCC Decision on Internet Charges
(CNN)

Update: In April 1999, a Canadian version of this message was
unleashed on the Internet.
Unlike its American counterpart, this version is not mere misinformation
based on a flawed
understanding of actual events or legislation -- it is an outright hoax:

Please read the following carefully if you intend to stay
online and continue using email:

The last few months have revealed an alarming trend in the
Government of Canada attempting to quietly push through
legislation that will affect your use of the Internet. Under
proposed legislation Canada Post will be attempting to bilk
email users out of "alternate postage fees".

Bill 602P will permit the Federal Govt to charge a 5 cent
surcharge on every email delivered, by billing Internet
Service Providers at source. The consumer would then be
billed in turn by the ISP. Toronto lawyer Richard Stepp QC is
working without pay to prevent this legislation from
becoming law.

The Canada Post Corporation is claiming that lost revenue
due to the proliferation of email is costing nearly
$23,000,000 in revenue per year. You may have noticed
Canada Post's recent ad campaign "There is nothing like a
letter". Since the average citizen received about 10 pieces
of email per day in 1998, the cost to the typical individual
would be an additional 50 cents per day, or over $180
dollars per year, above and beyond their regular Internet
costs. Note that this would be money paid directly to
Canada Post for a service they do not even provide. The
whole point of the Internet is democracy and
non-interference. If the Canadian Government is permitted
to tamper with our liberties by adding a surcharge to email,
who knows where it will end. You are already paying an
exhorbitant price for snail mail because of beaurocratic
inefficiency. It currently takes up to 6 days for a letter to be
delivered from Mississauga to Scarborough. If Canada Post
Corporation is allowed to tinker with email, it will mark the
end of the "free" Internet in Canada. One back-bencher,
Liberal Tony Schnell (NB) has even suggested a "twenty to
forty dollar per month surcharge on all Internet service"
above and beyond the government's proposed email
charges. Note that most of the major newspapers have
ignored the story, the only exception being the Toronto Star
that called the idea of email surcharge "a useful concept
who's time has come" (March 6th 1999 Editorial) Don't sit
by and watch your freedoms erode away! Send this email to
all Canadians on your list and tell your friends and relatives
to write to their MP and say "No!" to Bill 602P.

Kate Turner
Assistant to Richard Stepp QC
Berger, Stepp and Gorman
Barristers at Law
216 Bay Street
Toronto, ON
MlL 3C6

Yes, Americans are not alone in their belief that when the need for the
primary service
provided by a governmental agency diminishes or disappears, that agency
will come up with
draconian schemes to perpetuate its existence at taxpayer expense rather
than modernizing
or closing up shop. In this case, however, the message is simply too
riddled with errors to be
anything but a hoax:

There is no "Bill 602P" currently before the Canadian parliament.
The designations of
Canadian parliamentary bills take the form of the letter 'C' or
'S' followed by a
number (depending upon whether they originated in the House of
Commons or the
Senate). Besides having the wrong prefix, this purported bill is
assigned a number far
too high to be one currently being considered in parliament, as
you can see on the list
of Canadian government bills.

Despite the claim in the message, nothing about this alleged bill
is to be found. Also,
there was no editorial about this on the 6 March 1999 OpEd page of
Toronto
Star. (Maybe "major papers have ignored the story" because it's a
work of
fiction?)

There is no Richard Stepp QC; law firm by the name of Berger,
Stepp and Gorman; or
216 Bay Street in Toronto.

A list of Canadian Members of Parliament contains no MP by the
name of Tony
Schnell.

Of course, it didn't take long before the same thing started circulating
in an Americanized
version:

Dear Internet Subscriber:

Please read the following carefully if you intend to stay
online and continue using email: The last few months have
revealed an alarming trend in the Government of the United
States attempting to quietly push through legislation that
will affect your use of the Internet. Under proposed
legislation the U.S. Postal Service will be attempting to bilk
email users out of "alternate postage fees".

Bill 602P will permit the Federal Govt to charge a 5 cent
surcharge on every email delivered, by billing Internet
Service Providers at source. The consumer would then be
billed in turn by the ISP. Washington D.C. lawyer Richard
Stepp is working without pay to prevent this legislation
from becoming law.

The U.S. Postal Service is claiming that lost revenue due to
the proliferation of email is costing nearly $230,000,000 in
revenue per year. You may have noticed their recent ad
campaign "There is nothing like a letter". Since the average
citizen received about 10 pieces of email per day in 1998,
the cost to the typical individual would be an additional
50 cents per day, or over $180 dollars per year, above and
beyond their regular Internet costs. Note that this would be
money paid directly to the U.S. Postal Service for a service
they do not even provide. The whole point of the Internet is
democracy and non-interference. If the federal government
is permitted to tamper with our liberties by adding a
surcharge to email, who knows where it will end. You are
already paying an exorbitant price for snail mail because of
bureacratic efficiency. It currently takes up to 6 days for a
letter to be delivered from New York to Buffalo. If the U.S.
Postal Service is allowed to tinker with email, it will mark
the end of the "free" Internet in the United States. One
congressman, Tony Schnell (r) has even suggested a
"twenty to forty dollar per month surcharge on all Internet
service" above and beyond the government's proposed
email charges. Note that most of the major newspapers
have ignored the story, the only exception being the
Washingtonian which called the idea of email surcharge "a
useful concept who's time has come" (March 6th 1999
Editorial) Don't sit by and watch your freedoms erode away!

Send this email to all Americans on your list and tell your
friends and relatives to write to their congressman and say
"No!" to Bill 602P.

Kate Turner
Assistant to Richard Stepp
Berger, Stepp and Gorman
Attorneys at Law
216 Concorde Street,
Vienna, Va.



From daemon Thu Feb 10 19:05:48 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Thu, 10 Feb 2000 09:03:00 -0800
Subject: paraffin sectioning troubleshooting guide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know where I can find a troubleshooting manual/guide for
paraffin sectioning? I am having trouble with the tissue "smearing" and
with it looking chopped-up. Is this a vibration issue? I welcome any
comments from those of you with experience in this area!

Thanks in advance.

Sincerely.



From daemon Thu Feb 10 19:05:51 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Thu, 10 Feb 2000 09:04:17 -0800
Subject: Fwd: paraffin sectioning troubleshooting guide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, I forgot to sign my note last time...


} Date: Thu, 10 Feb 2000 09:03:00 -0800
} To: microscopy-at-sparc5.microscopy.com
} From: Laurie Wallin {lwallin-at-ucsd.edu}
} Subject: paraffin sectioning troubleshooting guide
} Cc:
} Bcc:
} X-Attachments:
}
} Does anyone know where I can find a troubleshooting manual/guide for
} paraffin sectioning? I am having trouble with the tissue "smearing" and
} with it looking chopped-up. Is this a vibration issue? I welcome any
} comments from those of you with experience in this area!
}
} Thanks in advance.
}
} Sincerely.

Laurie Wallin
UCSD Department of Anesthesiology
9500 Gilman Drive, 0629, La Jolla, CA 92093
(858) 822-3271



From daemon Thu Feb 10 19:06:00 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 10 Feb 2000 12:31:07 -0500
Subject: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Catalano:

There has been some very significant work done by Dr. Arpad Barna et al in
Hungary. Some of the papers he has presented include:

Ion Energy Effect on Surface Amorphisation of Semiconductor Crystals/A.
Barna, et al

Amorphisation and Surface Morphology Development at Low Energy Ion
Milling/A. Barna, B. Pecz.

Analysis of the Development of Large Surface Topography During Ion
Etching/A. Barna; P. Barna; et al

Model Considerations of Ion Beam Thinning for Preparing TEM
Samples/A.Barna; et al

Possibility of Surface Polishing by Ion Beam Thinning/ A. Barna

Ion Beam Thinning on the Basis of Topographic Kinetics/A. Barna

Low Angle and Low Energy Ion Beam Etching for TEM Sample Preparation/A.
Barna

TEM Sample Preparation by Ion Milling / Amorphization/A. Barna, B. Pecz, M.
Menyhard

I also have the following paper that may be of interest:

Preparation of InGaAs/GaAs Multilayered Materials for TEM by One Side
Non-Rotation Ion Beam Thinning/J.Y. Yao; G.L. Dunlop

I do not have the complete references available in front of me, but we do
have copies of all of these papers in our technical library. I would be
pleased to mail copies to you if that would be of interest. We also have a
list of over 250 papers dealing with various aspects of sample preparation
(much of it TEM sample preparation) which may be of interest. If you would
like to review that list, I can send it over to you in MS Excel format and
you can select any other papers that would be of interest.

I hope this helps.

DISCLAIMER: South Bay Technology, Inc. markets the IV3 Ion Mill in both
high and low energy (down to 200ev) versions which is based on the work of
Dr. Barna. We also produce the XLA 2000 computer controlled Low Angle Ion
Mill so we have a vested interest in promoting their use.

Best regards-

David
Writing at 10:05:16 AM on 02/09/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Massimo Catalano
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listservers,

we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.

Specimen preparation is of course a crucial point of our work.

We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.

We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.

Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.

Thanks.

Massimo




Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm


{



From daemon Thu Feb 10 19:06:02 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 10 Feb 2000 09:42:05 -0800 (PST)
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, this urban legend has finally made it to the microscopy listserver!
And that is just what it is--a legend, a myth, a fable. As a stamp
collector and one who keeps abreast of all of this kind of stuff, this urban
legend has been debunked in both Linn's Stamp News and Stamp Collector.

All of us have important issues to consider. Please, put this one to rest.

Roger Moretz


On Wed, 9 Feb 2000 21:58:18 -0700, Christina bennett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I know this is off topic however a change like this could make this type
of
} forum impossible.
}
}
}
}
}
} } Date: Tue, 8 Feb 2000 08:13:53 -0700
} } X-Sender: liperez-at-cnmailsvr.nmsu.edu
} } Mime-Version: 1.0
} } To: all-at-biology.nmsu.edu
} } From: liperez-at-NMSU.Edu (LSPSaldana)
} } Subject: Congress to allow email charges
} } Sender: owner-all-at-biology.nmsu.edu
} } Precedence: bulk
} } X-Keywords:
} } X-UID: 43
} } Status: O
} }
} }
} } } } Congress to allow email charges
} } } }
} } } } Please pass this on to all you know since many of us use e-mail for
} } } } business and to keep up with friends and family, I thought you'd like
} } } } to know the following.
} } } }
} } } } Please jump on it right away and forward this to others.
} } } }
} } } } CNN has reported that within the next two weeks Congress is going to
} } } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } } internet access.
} } } } Translation: Every time we send long distance e-mail we will receive
a
} } } } long distance charge. This will get costly. Please visit the
following web
} } } } site and file a complaint. Complain to your Congressperson. We can't
} } } } allow this to pass. The following address will allow you to send an
} } } } e-mail on this subject DIRECTLY to your Congressperson.
} } } }
} } } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } } }
} } } } Pass this on to your friends. It is urgent! I hope all of you will
pass
} } } } this on to all your friends and family. We should ALL have
} } } } an interest in this one.
} } } }
} } } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } } alarming trend in the Government of the United States attempting to
} } } } quietly push through legislation that will affect your use of the
} } } Internet.
} } } } Under
} } } } proposed legislation the U.S. Postal Service will be attempting to
bill
} } } } email users out of "alternate postage fees". Bill 602P will permit
the
} } } } Federal Govt. to charge a 5 cent surcharge on every email delivered,
by
} } } } billing Internet Service Providers at source. The consumer would then
be
} } } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } } working without pay to prevent this legislation from becoming law.
The
} } } U.S.
} } } } Postal Service is claiming that lost revenue due to the proliferation
of
} } } } email
} } } } is costing nearly $230,000,000 in revenue per year. You may have
noticed
} } } } their recent ad campaign "There is nothing like a letter".
} } } } Since the average citizen received about 10 pieces of email per day
in
} } } } 1998,
} } } } the cost to the typical individual would be an additional 50 cents
per
} } } } day, or over $180 dollars Per year, above and beyond there regular
} } } Internet
} } } } costs.
} } } } Note that this would be money paid directly to the U.S. Postal
Service
} } } } for a service they do not even provide. The whole point of the
Internet is
} } } } democracy and non-interference. If the federal government is
permitted
} } } } to tamper with our liberties by adding a surcharge to email, who
knows
} } } Where
} } } } it will end. You are already paying an exorbitant price for snail
mail
} } } } because of bureaucratic inefficiency. It currently takes up to 6 days
for
} } } a
} } } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } } Service
} } } } is
} } } } allowed to tinker with email; it will mark the end of the "free"
} } } } Internet in the United States.
} } } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } } dollar per month surcharge on all internet service" above and beyond
the
} } } } government's proposed email Charges. Note that most of the major
} } } } newspapers have ignored the story, the only exception being the
} } } } Washingtonian
} } } } which called the idea of email surcharge "a useful concept who's time
has
} } } } come"
} } } } (March 6, 1999) Editorial.
} } } }
} } } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } } EVERYONE on your list, and tell all your friends and relatives to
write
} } } } to their congressman and say "No!" to Bill 602P.
} } } }
} } } }
} } } } It will only take a few moments of your time, and could very well be
} } } } instrumental in killing a bill we don't want.
} } } }
} } } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE
TWO
} } } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } } NOW, NOT AFTER
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Thu Feb 10 19:06:11 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 10 Feb 2000 11:45:28 -0600
Subject: GFP and Osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know if GFP is quenched after OsO4 postfixation?
Hank Adams
Integrated Microscopy Core
Molecular and Cell Biology
Baylor College of Medicine
Houston, TX 77030



From daemon Thu Feb 10 19:06:16 2000



From: Carlos Kazuo Inoki :      kazuo-at-csc.albany.edu
Date: Thu, 10 Feb 2000 13:05:16 -0500 (EST)
Subject: TEM: sample preparation for InSb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm working with a InSb sample prepared with tripod polishing.
Since the layer is quite thick, more than 3 micron plus a 2 micron buffer
layer, I am experimenting some difficult to prepare a good sample for
cross section observation. The final polishing is done in a Gatan ion
milling set using a low angle. The problem with my samples is how to get a
uniform thickness from the top of the sample to the substrate. Usually the
top of the sample is milled much faster than near to the substrate. I
can't mechanically polish the sample too much, since the sample is
somewhat fragile (brittle). Even in old papers people report this kind of
problem when preparing InSb samples for TEM using ion milling. Does
someone else suffered from this kind of problem: the top part of a
sample being milled away? Oh yes, when I polish the sample I try to make
wedge perpendicular to the sample surface, so the thickness of the sample
is the same from the InSb layer to the substrate after the mechanical
polishing. There is some correlation between the ion milling angle/energy
with this effect? The Gatan epoxy I use to glue a Si piece on top of the
sample should have some influence (charging)? Thanks.

Kazuo

o-------------------------------------------------------o
| Carlos Kazuo Inoki |
| Department of Physics - University at Albany |
| 1400 Washington Ave.- Albany - NY - 12222 |
o-------------------------------------------------------o



From daemon Thu Feb 10 19:06:25 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 10 Feb 2000 13:51:03 -0500
Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row elemen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that I know understand the reason why you get Be X-ray and why
there are energy shifts when it is in different compounds. My next question
then is do the elements between B and F have measurable energy shifts
depending on what material they are in? The key word here is measurable.
The Ka1 and Ka2 peaks are due to transitions form the 2p1/2 and 2p3/2 (LII
and LIII) to the 1s1/2 state. But these would be valence/conductance bands
for the pure elements. (Assuming you collect your X-rays from solid N2, O2,
or F2) The band structure would be different for different materials. For
example, do you see any shifts between B2O3? and BN or C(diamond) and
C(graphite) or TiC? Any microprobers following this thread?

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Thursday, February 10, 2000 11:01 AM
} To: Walck. Scott D.; 'microprobe'
} Cc: 'Microscopy'
} Subject: RE: Be X-ray peaks -I don't think so
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear Scott,
}
} A poster does not mean anything. I have seen them with the
} Lithium-Ka energy
} listed, although quantum physics does forbid this X-ray line.
} But I can
} assure you the Be-K line does exist.
}
} On modern EDX systems Be can be detected, provided you have either a
} windowless detector or a detector with a modern polymer type
} window (SUTW,
} SATW, Norvar, or whatever the EDX supplier calls it. My
} apologies if I break
} any trade-marks here...).
} On most demonstration setups your EDS specialist will be
} pleased to show you
} a Be peak. But probably from a pure Be sample only. The absorption
} coefficient (MAC) of Be in basically any matrix is so huge,
} and the X-ray
} fluorescence yield so small, that in any compound with less
} than 50 atomic
} percent Be you will not detect a visible Be peak. For this
} reason most EDX
} installations "in the field" are not setup to detect Be-K radiation.
}
} WDS has a better P/B ratio, so if you have the proper
} multilayer crystal you
} can get results. But peak shifts and peak shape alterations will make
} quantification extremely difficult, not to mention the fact
} that for most
} MACs of Be-K in any matrix we only have a ball-park figure.
}
} Other techniques, like PEELS, are much more suitable for Li
} and Be analysis.
} Best regards,
}
} Hans Dijkstra
}
} Disclaimer: This is my opinion, and not necessarily the one
} from EDAX Inc.
} -------------------------------------------------------------
} EDAX Europe www.edax.com
} Ringbaan Noord 103 Tel.: +31-13-5364000
} P.O. Box 4144 Fax.: +31-13-5356279
} 5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
} the Netherlands
} -------------------------------------------------------------
}
}
} } -----Original Message-----
} } From: Walck. Scott D. [mailto:walck-at-ppg.com]
} } Sent: Tuesday, February 08, 2000 6:57 PM
} } To: 'microprobe'; 'harry.ekstrom-at-honeywell.com'
} } Cc: 'Microscopy'
} } Subject: RE: Be X-ray peaks -I don't think so
} }
} }
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
} }
}
} --------------------------------------------------------------
} ---------.
} }
} }
} } I'm wrong. I guess I should have looked up at the X-ray
} periodic table
} } above my head and saw the 0.108 keV value for Be. The
} reason that I was
} } wrong was that I did not consider the solid state aspects.
} I am still
} right
} } about not having a 2s1/2 to a 1s1/2 transition and that an
} X-ray carries
} } away 1h-bar of angular momentum. What I didn't do was
} think about the
} band
} } structure of a solid. If you have the solid, the 2p bands
} of Be most be
} } spilling into or be the conduction band for the electrons.
} That must be
} the
} } source for the electronic transition. I guess I should
} have engaged brain
} } before typing.
} } My most humble apologies.
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } PPG Industries, Inc.
} } Glass Technology Center
} } Guys Run Rd. (packages)
} } P. O. Box 11472 (letters)
} } Pittsburgh, PA 15238-0472
} }
} } Walck-at-PPG.com
} }
} } (412) 820-8651 (office)
} } (412) 820-8161 (fax)
} }
} }
} } "The opinions expressed are those of Scott D. Walck and not of PPG
} } Industries, Inc. nor of any PPG-associated companies."
} } --
} }
}
}



From daemon Thu Feb 10 19:06:27 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 10 Feb 2000 13:57:08 -0500
Subject: RE: sample preparation for InSb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I did InSb and AlInSb by the small angle cleavage technique when I was at
Wright Patterson AFB. I assume since you are using the Tripod Technique
that you require site-specific results. If not, try it. It works really
well on this material.

I am getting to sound like a broken record on this topic, aren't I? See my
post yesterday.

-Scott



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Carlos Kazuo Inoki [mailto:kazuo-at-csc.albany.edu]
} Sent: Thursday, February 10, 2000 1:05 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM: sample preparation for InSb
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi,
}
} I'm working with a InSb sample prepared with tripod polishing.
} Since the layer is quite thick, more than 3 micron plus a 2
} micron buffer
} layer, I am experimenting some difficult to prepare a good sample for
} cross section observation. The final polishing is done in a Gatan ion
} milling set using a low angle. The problem with my samples is
} how to get a
} uniform thickness from the top of the sample to the
} substrate. Usually the
} top of the sample is milled much faster than near to the substrate. I
} can't mechanically polish the sample too much, since the sample is
} somewhat fragile (brittle). Even in old papers people report
} this kind of
} problem when preparing InSb samples for TEM using ion milling. Does
} someone else suffered from this kind of problem: the top part of a
} sample being milled away? Oh yes, when I polish the sample I
} try to make
} wedge perpendicular to the sample surface, so the thickness
} of the sample
} is the same from the InSb layer to the substrate after the mechanical
} polishing. There is some correlation between the ion milling
} angle/energy
} with this effect? The Gatan epoxy I use to glue a Si piece on
} top of the
} sample should have some influence (charging)? Thanks.
}
} Kazuo
}
} o-------------------------------------------------------o
} | Carlos Kazuo Inoki |
} | Department of Physics - University at Albany |
} | 1400 Washington Ave.- Albany - NY - 12222 |
} o-------------------------------------------------------o
}
}



From daemon Thu Feb 10 19:06:30 2000



From: David_Bell-at-Millipore.com
Date: Thu, 10 Feb 2000 14:06:06 -0500
Subject: My apologies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello again,

My apologies to the list and especially those at RJ Lee! Hope nobody was
offended.

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Thu Feb 10 19:06:33 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Thu, 10 Feb 2000 13:16:34 -0600
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Woody,

I hate to add to the bandwidth clutter but these are very old hoaxes.

Damian


} Is this yet another hoax eating time & bandwidth by being posted without
} confirmation (like the last 3-4 times I saw this message in the pas year or
} two), or is it real?



From daemon Thu Feb 10 19:06:51 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Thu, 10 Feb 2000 21:54:09 +0100 (MET)
Subject: Re: GFP and Osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hank,

Yes, after OsO4 GFP is quenched, moreover it is quenched significantly
(three times or more) even with 0.05% of glutaraldehyde (1Ž0 min.


Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it


On Thu, 10 Feb 2000, hank adams wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone know if GFP is quenched after OsO4 postfixation?
} Hank Adams
} Integrated Microscopy Core
} Molecular and Cell Biology
} Baylor College of Medicine
} Houston, TX 77030
}
}
}



From daemon Fri Feb 11 18:28:57 2000



From: Fagerland,Jane :      jane.a.fagerland-at-abbott.com
Date: Thu, 10 Feb 2000 15:13:19 -0600
Subject: Summer Internships at Abbott - more information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all of you who have shown an interest in our internships. I'd like
to provide a few more details and answer some questions I've received so far
about the summer internships at Abbott.

First: Are the internships open to international students? Technically,
yes. However, interns are employees of Abbott Laboratories during their time
here. Thus, the logistics of processing the necessary paperwork for non-US
citizens for a 12-week employment period would not be practical. So, in
truth, the answer has to be that, except in highly unusual circumstances, we
will limit applications to students in the United States.

Second: Are the internships intended for college or high school students?
The internships are for college students working towards a Bachelor's degree
or higher. Students in the Associate degree programs at Madison Area
Technical College and San Joaquin Delta College are eligible, but priority
will be given to students who already have a Bachelor's degree.

Third: How to apply? Go to the Abbott Laboratories website at abbott.com
and follow the links: Careers, Entry Level Positions, Summer Internship
Program. There you will find an electronic resume form that can be submitted
from your computer. THE DEADLINE FOR SUBMISSION IS MARCH 1.

Along with submitting the electronic resume, please send me either an
e-mailed or faxed copy of your resume, or a hardcopy via snail mail. The
electronic resume ends up in Corporate Staffing, where your skills will be
matched with the internship openings at Abbott. If I have a copy of your
resume, I can make sure that you are considered for positions in the
microscopy department.

If there are any other questions, please feel free to contact me.

Thanks,

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
D-45M/AP31 200 Abbott Park Rd.
Abbott Park IL 60064-6202
voice: (847) 935-0104
fax: (847) 938-5027
e-mail: jane.a.fagerland-at-abbott.com



From daemon Thu Feb 10 19:06:46 2000



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 10 Feb 2000 14:04:19 -0800 (PST)
Subject: Re: autofluorescence quenching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello from Seattle,

There was a paper by Cowen, Haven and Burnstock 1985, using Pontamine Sky
Blue couterstain to reduce autofluorescence.

Also a paper by Kittleburger, Davis, and Stephans in Acta Histochem 89,
using Eriochrome Black T.

Bob
Morphology Core
U of Washington

On Wed, 9 Feb 2000, Corazon Bucana wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working with archival specimens of formalin-fixed paraffin embedded
} tissues and I am getting tremendous autofluorescence. I looked at tissue
} sections after the sections have been de-waxed using either FITC or
} rhodamine filter sets and red cells and connective tissues are brightly
} fluorescent. Is there a way of suppressing the autofluorescence and still
} retain reactivity of tissue to antibodies? I would appreciate any comments
} or suggestions.
}
} *******************************************************
} Corazon D. Bucana, Ph.D.
} Department of Cancer Biology
} U.T. M.D. Anderson Cancer Center
} 1515 Holcombe Blvd. Box 173
} Houston, Texas 77030
} Phone: (713) 792-8106
} FAX: (713) 792-8747
} Email:bucana-at-audumla.mdacc.tmc.edu
} FAX: (713) 792-8747
}
}
}



From daemon Thu Feb 10 19:06:49 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 11 Feb 2000 10:01:58 +1100
Subject: Re: New Gold Enhancement Reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 8:51 AM +0100 4/2/00, Alexander Mironov Jr. wrote:
} I have tried GoldEnhance for preembedding protocol to label intracellular
} structures. It is marvelous. Buy it and use it - you will not be
} unsatisfied. I have no any interest in Nanoprobes, I just like these dense
} gold particles.
} Practically all they claim is true:
} - light insensitive
} - no self-nucleation
} - do not react with buffer ions
} - is not dissolved by uranil and osmium (I have treat for 1 h)

I have been using BioCell's silver enhancement kit for years and
particularly like it for the light insensitivity - one can monitor
the reaction under the light microscope. Is it possible to do this
with the GoldEnhance as well?

GoldEnhance sounds perfect - has anyone had any problems with it?

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Fri Feb 11 18:29:01 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 10 Feb 00 20:26:38 -0800
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reply to: Re:FYI: Congress to allow email charges -Hoax?
I too had my suspicions. It looks very much like the "Internet Access Tax" letter from a few years ago. See: {http://urbanlegends.miningco.com/culture/urbanlegends/library/weekly/aa010798.htm} for details.

There are many sites documenting this sort of stuff. {http://www.hoaxkill.com/} is an example .

Disclaimer: I have no affiliation to this site.

Regards,
Paul Webster

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Feb 11 18:29:06 2000



From: Nelson+ Conti :      NelsonC51-at-excite.com
Date: Thu, 10 Feb 2000 21:07:34 -0800 (PST)
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





On Thu, 10 Feb 2000 13:16:34 -0600, Damian wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Woody,
}
} I hate to add to the bandwidth clutter but these are very old hoaxes.
}
} Damian
}
}
} } Is this yet another hoax eating time & bandwidth by being posted without
} } confirmation (like the last 3-4 times I saw this message in the pas year
or
} } two), or is it real?
}
}
Damian's response is indeed correct -- see posted responses by
"David_Bell-at-millipore.com", "EvexAnalyt-at-aol.com", Scott Wight, Kalman Rubin,
Dr. Gary Gaugler, donald j. marsh, Roger Moretz, Laurie Wallin and Damian.
Being new to the MSA listserver, I'm very relieved that such notices (err,
BANDWIDTH-OCCUPYING, ANNOYING HOAXES) are determined to be false by very
astute readers. I fell for this, and, upon reading other people's notices, I
am upset that someone would suggest spreading such hoaxes around like the
classic junk chain mail activities done at school (or even college). Such
notices clog up the space needed for important issues regarding microscopy.
Anyway .. I'm responding merely to alert other GULLIBLE people (like myself)
not to believe such notices, as well as be thankful that this has been a
recurring event in the MSA listserver.
Nelson Conti





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Fri Feb 11 18:29:18 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Fri, 11 Feb 2000 10:31:02 +0100 (MET)
Subject: Re: New Gold Enhancement Reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Diana,

IMHO, the main problem with GoldEnhance is some inhomogenity in particle
size but for my purposes it is irrelevant. Also the kit works good in my
hands at pH 7.4 but does not at pH 6.5 (as claimed Nanoprobes).

Sincerely,
Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it





From daemon Fri Feb 11 18:29:19 2000



From: GUIDO LŸšND :      lueoend-at-zzmk.unizh.ch
Date: Fri, 11 Feb 2000 10:31:14 +0100
Subject: TEM Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear readers,
could anyone give me some hints or tips how I can separate a formvarfilm
(0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
help at all. It works better with non-washed slides, but then I get an
uneven and dirty surface of the film.
I appreciate every kind of support.
Best regards

Guido Luond
Institute of Oral Microbiology and
General Immunology
Univ. of Zurich
Plattenstr. 11
CH-8028 Zurich, Switzerland
E-mail: lueoend-at-zzmk.unizh.ch



From daemon Fri Feb 11 18:29:21 2000



From: richard.beanland-at-gecm.com
Date: Fri, 11 Feb 2000 09:48:48 +0000 (GMT)
Subject: Re: TEM: Sample preparation for InSb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Carlos,
sounds like you may be having a problem with differential milling rates. Try milling over a restricted angle; you can use oscillation (if your ion mill is set up to do this) or make C-shaped Ta plate shields which you fix on the sample holding plates of your ion mill, with the sample at the centre of the 'C'. If you put your sample in the plates such that the interface is parallel to the opening of the shield, the average direction of the ion beam will be perpendicular to the interface. This will prevent the ion beam milling parallel to the glue line and stop the layers disappearing before the substrate. If you stick to angles below 10 degrees you will get better results, and of course use liquid nitrogen cooling and/or iodine. My apologies if this isn't very clear - it's hard to describe everything with just words! I can send you a picture of the sample holder I use if you like.

Best regards,

Richard Beanland

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I'm working with a InSb sample prepared with tripod polishing.
} Since the layer is quite thick, more than 3 micron plus a 2 micron buffer
} layer, I am experimenting some difficult to prepare a good sample for
} cross section observation. The final polishing is done in a Gatan ion
} milling set using a low angle. The problem with my samples is how to get a
} uniform thickness from the top of the sample to the substrate. Usually the
} top of the sample is milled much faster than near to the substrate. I
} can't mechanically polish the sample too much, since the sample is
} somewhat fragile (brittle). Even in old papers people report this kind of
} problem when preparing InSb samples for TEM using ion milling. Does
} someone else suffered from this kind of problem: the top part of a
} sample being milled away? Oh yes, when I polish the sample I try to make
} wedge perpendicular to the sample surface, so the thickness of the sample
} is the same from the InSb layer to the substrate after the mechanical
} polishing. There is some correlation between the ion milling angle/energy
} with this effect? The Gatan epoxy I use to glue a Si piece on top of the
} sample should have some influence (charging)? Thanks.
}
} Kazuo
}
} o-------------------------------------------------------o
} | Carlos Kazuo Inoki |
} | Department of Physics - University at Albany |
} | 1400 Washington Ave.- Albany - NY - 12222 |
} o-------------------------------------------------------o
}

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Fri Feb 11 18:29:29 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 11 Feb 2000 07:50:18 -0500
Subject: Re: Color vs. monochrome cameras - a different approach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

Is your end goal hi res imaging or ratioing? If the latter, I saw an
interesting technology at Cell Bio which might be of help: the PARISS
Spectral imaging system from LightForm. I saw very early versions of this
technology some years ago at PITTCON but it has really matured and offers
an interesting alternative. PARISS can acquire 240 spectra simultaneously,
full field or in a region of interest. It really crashes through the old
barriers imposed by filter cubes. Also, despite the radical differences in
intensity, it can acquire the transmitted light image simultaneously with
the fluorescence image and present the combined results with perfect
registration. The system retrofits to existing microscopes and is
moderately priced, considering all the accessories (2 cameras,
spectrometer, motorized stage, software). If you are interested, visit
their website at lightforminc.com or contact Jeremy Lerner (908-281-9098).

Caveat: MME has no financial interest in this product.

Hope this is helpful.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



At 10:23 AM 2/10/00 -0500, David Knecht wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Feb 11 18:29:30 2000



From: L. D. Marks :      ldm-at-apollo.numis.nwu.edu
Date: Fri, 11 Feb 2000 07:35:08 -0600 (CST )
Subject: Re: coherence and spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, I think a small correction/clarification is requires. This is ONLY
for an incoherently filled condensor aperture, i.e. a large beam with C2
fully focussed. This approximation is not true with the newer microscopes!

On Thu, 10 Feb 2000, Jonathan Barnard wrote:

} Born & Wolf (section 10.4.2)
} "Hence if the linear dimensions of the source and the distance between P1
} and P2 (points in the imaging plane) are small compared to the distance of
} these points from the source, the degree of coherence, |mu_12| is equal to
} the absolute value of the normalized Fourier transform of the intensity
} function of the source"
}

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Fri Feb 11 18:29:38 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Fri, 11 Feb 2000 14:51:03 +0100
Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row elements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Scott,

WDS spectra of Boron, Carbon, Nitrogen and Oxygen in many compounds have
been extensively recorded by Bastin and Heijligers in the 1980's and early
1990's. They report that peak shifts and peak shape alterations are clearly
present in Boron compounds. In the case of TiB crystals it was even found
that the crystal orientation influences the peak shape and position, i.e. if
you rotate the sample 90 degrees you get a different peak shape. For Carbon
the peak shapes are practically unaffected by the bonding with other
elements.

Please check "Quantitative Electron Probe Microanalysis of Boron in Binary
Borides" by Bastin and Heijligers, University of Technology Eindhoven, the
Netherlands, 1997, ISBN 90-3860-898-5.

In EDX the peak shifts are undetectable. Modern EDX systems give a FWHM of
around 60-65 eV for these very light elements, so a peak shift of a few eV
is basically undetectable. For many users this makes EDX the prefered
technique to quantify Boron compounds, since with WDS you need to record
either the full peak (tedious) or use fixed area-peak-fraction correction
methods. In EDX we can simple ignore peak shape and peak position changes.

Best regards,

Hans Dijkstra

Disclaimer: This is my opinion, and not necessarily the one from EDAX Inc.
-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
} From: Walck. Scott D. [mailto:walck-at-ppg.com]
} Sent: Thursday, February 10, 2000 7:51 PM
} To: 'Microscopy'
} Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row
} elements
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I think that I know understand the reason why you get Be X-ray and why
} there are energy shifts when it is in different compounds. My next
question
} then is do the elements between B and F have measurable energy shifts
} depending on what material they are in? The key word here is measurable.
} The Ka1 and Ka2 peaks are due to transitions form the 2p1/2 and 2p3/2 (LII
} and LIII) to the 1s1/2 state. But these would be valence/conductance
bands
} for the pure elements. (Assuming you collect your X-rays from solid N2,
O2,
} or F2) The band structure would be different for different materials. For
} example, do you see any shifts between B2O3? and BN or C(diamond) and
} C(graphite) or TiC? Any microprobers following this thread?
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}



From daemon Fri Feb 11 18:29:33 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Fri, 11 Feb 2000 15:08:23 +0100
Subject: PIPS and milling damage. Second message.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers:

first of all I would like to thank of the people who spent part of their
time trying to share their knowledge with me, and also to the manufacturers
who of course replied my messages.

All messages contained very important suggestions, that I need to study and
evaluate, and I will contact personally the people that offered to share
their previous experience and/or to collaborate.

Our work on InGaAs/GaAs quantum wells, single and stacked quantum wires,
single and stacked quantum dots, consists of conventional TEM, high
resolution transmission electron microscopy and high spatial resolution
analytical electron microscopy, that we perform in close collaboration with
Arizona State University, using a FEG/STEM machine equipped with EELS and
EDX. One of the goals of the research is to evaluate compositional
fluctuations in the well and in the dots at nanoscale.

As everybody knows, the requirements from a TEM sample for imaging are
completely different from the requirements necessary to perform high
spatial resolution analytical work, were effects such as amorphisazion,
surface contamination are critical.

We have a pretty well estabilished specimen preparation technique, but
before publishing results into the scientific community, we want to be sure
that what we observe is what is in the sample (isn't this the main concern
of every microscopist?).

That's why all suggestions, opinions and critics are strongly appreciated.

Best regards

Massimo


Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm




From daemon Fri Feb 11 18:29:35 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Fri, 11 Feb 2000 09:13:34 -0500 (EST)
Subject: Re: TEM Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't wash the slides, just wipe the dry slide with a Kim-Wipe a few
times. Works like a charm, whatever the weather! (Very grey and nasty here
on "the island" today, by the way).

Tamara Howard
CSHL


On Fri, 11 Feb 2000, GUIDO L[ISO-8859-1] üöND wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear readers,
} could anyone give me some hints or tips how I can separate a formvarfilm
} (0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
} help at all. It works better with non-washed slides, but then I get an
} uneven and dirty surface of the film.
} I appreciate every kind of support.
} Best regards
}
} Guido Luond
} Institute of Oral Microbiology and
} General Immunology
} Univ. of Zurich
} Plattenstr. 11
} CH-8028 Zurich, Switzerland
} E-mail: lueoend-at-zzmk.unizh.ch
}
}
}



From daemon Fri Feb 11 18:29:36 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 11 Feb 2000 08:27:09 -0600
Subject: Re: TEM: Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Guido,

There is a product called "Victawet", which is available here in the U.S.
from various EM suppliers. It comes in a small vial that lasts forever and
is an off-white, waxy or soapy substance. We used to take our slides and
clean them very well with lint-free papers, then place them in a rack inside
a vacuum evaporator, with the side intended for the Formvar film facing a
tungsten basket connected to the electrodes. Then we place a piece of
Victawet about the size of a grain of rice in the basket, pump down the
system to high vacuum, and slowly heat the tungsten basket until it glows
dull red. (If you increase the current too quickly the little piece will
jump out of the basket.) The slides will begin to look fogged, as if
someone had breathed moisture onto them. You can stop at that point---you
only need a little.

Store these coated slides until needed, then take them as required and
polish them very carefully with lint-free paper. They should feel slippery.
Use these cleaned slides to dip into the Formvar solution and the film
should separate easily.

Please note that the type of slide and the humidity levels in the workplace
also have a large effect. I have worked in places where films would
separate from any slide, every day, with no Victawet coating. I have also
worked in places where we could only get Formvar films on occasion (who
knows why?), and then we made a bunch of them at once.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-8304
(573) 884-5414 (fax)
email: tindallr-at-missouri.edu {mailto:tindallr-at-missouri.edu}
http://biotech.missouri.edu/emc {mailto:tindallr-at-missouri.edu}



Dear readers,

could anyone give me some hints or tips how I can separate a formvarfilm

(0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't

help at all. It works better with non-washed slides, but then I get an

uneven and dirty surface of the film.

I appreciate every kind of support.

Best regards

Guido Luond

Institute of Oral Microbiology and

General Immunology

Univ. of Zurich

Plattenstr. 11

CH-8028 Zurich, Switzerland

E-mail: lueoend-at-zzmk.unizh.ch


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-8304
(573) 884-5414 (fax)
email: tindallr-at-missouri.edu {mailto:tindallr-at-missouri.edu}
http://biotech.missouri.edu/emc {mailto:tindallr-at-missouri.edu}




From daemon Fri Feb 11 18:29:40 2000



From: mike.santanajr-at-amd.com
Date: Fri, 11 Feb 2000 08:48:14 -0600
Subject: RE: FYI: Congress to allow email charges -Hoax? (STOP)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,

Hasn't anyone realized that the "hoax" email is a success even as a
failure. Everyone is responding so much about the email being a hoax that
we're using up band width and cluttering email accounts. We all know it's a
hoax..., let's leave it at that.....


Regards,

Mike Santana, Jr.
Fab25 Product Engineering
Advanced Micro Devices
Desk - (512) 602-4172
Pager - (512) 622-2494
email - mike.santanajr-at-amd.com

On Thursday, February 10, 2000 10:27 PM, Paul Webster
[SMTP:pwebster-at-hei.org] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Reply to: Re:FYI: Congress to allow email charges -Hoax?
} I too had my suspicions. It looks very much like the "Internet Access
Tax" letter from a few years ago.
See: {http://urbanlegends.miningco.com/culture/urbanlegends/library/weekly/aa
010798.htm} for details.
}
} There are many sites documenting this sort of stuff.
{http://www.hoaxkill.com/} is an example .
}
} Disclaimer: I have no affiliation to this site.
}
} Regards,
} Paul Webster
}
} Paul Webster, Ph.D.
} Associate Scientist & Director
} Ahmanson Advanced Electron Microscopy & Imaging Center
} House Ear Institute
} 2100 West Third St.
} Los Angeles, CA 90057
}
} Phone: (213) 273-8026
} Fax: (213) 413-6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm
}



From daemon Fri Feb 11 18:29:48 2000



From: Jesœs Ricote :      jricote-at-icmm.csic.es
Date: Fri, 11 Feb 2000 16:21:51 +0100
Subject: ion thinner: users of Bal-Tec RES 010

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listmembers,

I would like to contact users of Bal-Tec RES 010 ion thinner to comment on
performance of this equipment. We have found over the years frequent
stability problems of the guns, which are not solved only with cleaning. We
are at the moment checking any possible source of unstability. We would
like advise from anyone who has had similar problems with this apparatus,
to know if we are missing something and what would be the best course of
action.

Thanks in advance

Jesœs Ricote
-------------------------------------------------------------
Dr. Jesœs Ricote
Departamento de Materiales FerroelŽctricos
Instituto de Ciencia de Materiales de Madrid
Consejo Superior de Investigaciones Cientificas (CSIC)
Cantoblanco
28049 Madrid
SPAIN

Phone: ( + 34) 91 334 90 00 (Ext. 268)
Fax: ( + 34) 91 372 06 23
e-mail: jricote-at-icmm.csic.es
http://www.icmm.csic.es/mf/ferroesp.htm
-------------------------------------------------------------



From daemon Fri Feb 11 18:29:47 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Fri, 11 Feb 2000 16:22:30 +0100
Subject: PIPS and milling damage: Second message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers:

first of all I would like to thank of the people who spent part of their
time trying to share their knowledge with me, and also to the manufacturers
who of course replied my messages.

All messages contained very important suggestions, that I need to study and
evaluate, and I will contact personally the people that offered to share
their previous experience and/or to collaborate.

Our work on InGaAs/GaAs quantum wells, single and stacked quantum wires,
single and stacked quantum dots, consists of conventional TEM, high
resolution transmission electron microscopy and high spatial resolution
analytical electron microscopy, that we perform in close collaboration with
Arizona State University, using a FEG/STEM machine equipped with EELS and
EDX. One of the goals of the research is to evaluate compositional
fluctuations in the well and in the dots at nanoscale.

As everybody knows, the requirements from a TEM sample for imaging are
completely different from the requirements necessary to perform high
spatial resolution analytical work, were effects such as amorphisazion,
surface contamination are critical.

We have a pretty well estabilished specimen preparation technique, but
before publishing results into the scientific community, we want to be sure
that what we observe is what is in the sample (isn't this the main concern
of every microscopist?).

That's why all suggestions, opinions and critics are strongly appreciated.

Best regards

Massimo


Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm




From daemon Fri Feb 11 18:29:51 2000



From: richard.beanland-at-gecm.com
Date: Fri, 11 Feb 2000 15:56:43 +0000 (GMT)
Subject: Re: U-shaped Ta for IBT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ah. Bit of a problem, that. I didn't mention how far away the shield is from the sample - about 3 or 4 mm - and that for low angles I use a wedge-shaped shield to deflect ions away from the specimen, rather than back on to it. Did you really try it that quickly? I only sent the e-mail a few hours ago! I did expect anyone who was going to have a go to ask me for a picture of my holder.
As to cleaning your sample, I think you can only ion mill it some more, with amended shields...

Richard


} Dear Richard,
}
} I did as you suggested in my sample preparation. However, the Ta was
} sputtered on the surface of the
} specimen. Could you suggest me how to avoid or how to clean the Ta on the
} specimen surface.
}
} Chengge Jiao
} H.H.Wills Physics Lab
} University of Bristol, U.K
} c.g.jiao-at-bristol.ac.uk

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Fri Feb 11 18:29:52 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 11 Feb 2000 11:16:35 -0500
Subject: RE: PIPS and milling damage: Second message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the benefits from the small angle cleavage technique on the III-V
compounds is that often a "step-like" sample is produced. When this
happens, you have a perfect sample for both High resolution and analytical
work. In the very thin steps, you can do HREM and EELS. In the thicker
steps, you can do EDS analysis.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Massimo Catalano [mailto:massimo.catalano-at-ime.le.cnr.it]
} Sent: Friday, February 11, 2000 10:23 AM
} To: Microscopy-at-sparc5.Microscopy.Com
} Subject: PIPS and milling damage: Second message
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear Listservers:
}
} first of all I would like to thank of the people who spent
} part of their
} time trying to share their knowledge with me, and also to the
} manufacturers
} who of course replied my messages.
}
} All messages contained very important suggestions, that I
} need to study and
} evaluate, and I will contact personally the people that
} offered to share
} their previous experience and/or to collaborate.
}
} Our work on InGaAs/GaAs quantum wells, single and stacked
} quantum wires,
} single and stacked quantum dots, consists of conventional TEM, high
} resolution transmission electron microscopy and high spatial
} resolution
} analytical electron microscopy, that we perform in close
} collaboration with
} Arizona State University, using a FEG/STEM machine equipped
} with EELS and
} EDX. One of the goals of the research is to evaluate compositional
} fluctuations in the well and in the dots at nanoscale.
}
} As everybody knows, the requirements from a TEM sample for
} imaging are
} completely different from the requirements necessary to perform high
} spatial resolution analytical work, were effects such as
} amorphisazion,
} surface contamination are critical.
}
} We have a pretty well estabilished specimen preparation
} technique, but
} before publishing results into the scientific community, we
} want to be sure
} that what we observe is what is in the sample (isn't this the
} main concern
} of every microscopist?).
}
} That's why all suggestions, opinions and critics are strongly
} appreciated.
}
} Best regards
}
} Massimo
}
}
} Dr. Massimo Catalano
} CNR-IME
} Campus Universitario
} Via Arnesano
} 73100 Lecce - ITALY
} tel: + 39 0832 322362
} fax: + 39 0832 325299
} email: massimo.catalano-at-ime.le.cnr.it
} http://www.ime.le.cnr.it
} http://www.ime.le.cnr.it/sime/sime.htm
}
}
}



From daemon Fri Feb 11 18:29:56 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 11 Feb 2000 11:20:25 -0600 (CST)
Subject: Re: TEM Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
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I've always had good luck getting the film off by polishing the slide
first with Ross Optical Lens Tissue. My major professor taught me this,
and his explaination was that it has silicone in it. Once you have cast
the film onto the slide and allowed it to dry, cut through the film at the
edges and bottom of the slide by running a different slide along them.
Then breathe on the film and lower it into the water dish.

Good luck,
Heather Owen

p.s. I have no affiliation with the company that makes, or any of the EM
supply houses that sell Ross lens paper.

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Fri Feb 11 18:29:58 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 11 Feb 2000 09:41:58 -0800
Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row

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Dear Scott,
In the Microbeam Analysis 1985, G.F. Bastin and H.J.M. Heijligers have the
first article, entitled: "Quantitative Electtron-Probe Microanalysis of Very
Light Elements" (page 1). They have several articles in that and succeeding
years in which they try to analyse every element and compound in the B to F
range material that they can get to sit still under the EPMA beam. They did
a very valuable set of studies that is worth looking into if you want to do
any very light element analysis. They cover peak shifts, peak shape changes,
even crystallographic direction sensitivities.
At 01:51 PM 2/10/00 -0500, you wrote:

}
} I think that I know understand the reason why you get Be X-ray and why
} there are energy shifts when it is in different compounds. My next question
} then is do the elements between B and F have measurable energy shifts
} depending on what material they are in? The key word here is measurable.
} The Ka1 and Ka2 peaks are due to transitions form the 2p1/2 and 2p3/2 (LII
} and LIII) to the 1s1/2 state. But these would be valence/conductance bands
} for the pure elements. (Assuming you collect your X-rays from solid N2, O2,
} or F2) The band structure would be different for different materials. For
} example, do you see any shifts between B2O3? and BN or C(diamond) and
} C(graphite) or TiC? Any microprobers following this thread?
}
} -Scott
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Feb 11 18:30:00 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Fri, 11 Feb 2000 10:12:05 -0800
Subject: Re: questions for MSA members microwave

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Hello Casey/Marty
I cannot answer for the first question as we use Diotome knives and I have
no experience with the other.

But the answer to the second question is "Yes." At least for us it is. We
are a facility used by the Faculty of Medicine and Science so many and
varied samples come through here for electron, light, and confocal
microscopy. We have been working up some protocols for difficult specimens
such as nematodes, drosophila larvae and plant material. The power supply
allows control so that you don't have to do any other processing to the
specimens than toss them in the fixative. Since the fix and post fix enter
the specimens at low power ( {200 watts) without damaging the specimens
(full power is usually about 800 watts) we can fix in about 45 minutes for
something which would normally be in 24 hours. And we don't have to punch
holes in the specimen.

We have just been playing with the introduction of fluorescent dyes for
light microscopy and confocal in C. elegans, without the use of agents to
distrupt the membranes to get the dye in. So far we have got the timings
for beautiful staining of the tail region and partial staining of the
thicker sections. We still have to get it right but the initial experiments
are very positive.

No-one seems to know why the microwaves work, but the hypothesis is that at
low power, the cell membranes are "jiggled about" to let the fix/dye in
without distrupting them.

We are a very busy lab, and our experiments are constantly getting
interupted so it is taking longer than we'd like to pin down a finished
protocol.

I would be interested in receiving protocols from labs who have already got
it to work for their specimens. We are finding that every specimen is
different and the protcols have to be tweaked for each one.
Elaine

}
} A colleague would like your responses to these questions. I believe that I
} say something on the first question about a year ago and apologize for
} asking again.

} } Here's what I/we need to know.
} }
} } 1) Are Microstar diamond knives equal to Diatome. We have an offer that
} } will save us about $1200 on three knives ( one 3mm ultra with 45 degree
} } angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).
} }
} }
} } 2) Is the power controller feature on the Pelco Microwave Tissue processor
} } (model #3451) worth it? (costs about $1300 additional compared to the
} } Pelco 3450 without the power controller). I plan to do immunolocalization
} } work, but am not sure this power controller feature is necessary or worth
} } the cost.
} }
} } Casey

Marty Reed


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Fri Feb 11 18:30:02 2000



From: Maria.Fazio-Zanakis-at-aventis.com
Date: Fri, 11 Feb 2000 12:17:37 -0600
Subject: TEM Formvar Film Cast on Glass

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Dear Guido,
I have learned long ago not to use films cast on slides. It is easier
and much cleaner to spread your films on the surface of water in a trough.
You can vary the thickness by the amount of drops you use and chose the area
you want to place your grids. This method gave me beautiful and sturdy
films.
Regards,
Maria

Maria Fazio-Zanakis
Bioimaging and Molecular Histology
Aventis Pharmaceuticals
1-908-231-3357
Fax: 1-908-231-3962
Email: Maria.Fazio-Zanakis-at-Aventis.com


-----Original Message-----
} From: GUIDO LŸšND [mailto:lueoend-at-zzmk.unizh.ch]
Sent: Friday, February 11, 2000 4:31 AM
To: EM-Tips


Dear readers,
could anyone give me some hints or tips how I can separate a formvarfilm
(0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
help at all. It works better with non-washed slides, but then I get an
uneven and dirty surface of the film.
I appreciate every kind of support.
Best regards

Guido Luond
Institute of Oral Microbiology and
General Immunology
Univ. of Zurich
Plattenstr. 11
CH-8028 Zurich, Switzerland
E-mail: lueoend-at-zzmk.unizh.ch



From daemon Fri Feb 11 18:30:06 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 11 Feb 00 10:43:54 -0800
Subject: RE: TEM Formvar Film Cast on Glass

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 11 Feb 00 10:43:54 -0800
Subject: RE: TEM Formvar Film Cast on Glass

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Reply to: RE: TEM Formvar Film Cast on Glass
Hi Guido,

Here is my recipe which has worked on four continents at altitudes up to 2,000m and in all weathers (humid to dry, cold to hot). Plus, it does not use those very unscientific body fluids I often see being used on this list!

Dissolve the formvar in chloroform as a concentration higher than will make the correct film thickness (film thickness will vary with the speed the glass is drawn out of the solution as well as with formvar concentration).

Take a glass slide (it is important to use precleaned 25x75 mm slides, VWR Scientific, Cat number 48312-002) and dip it in absolute ethanol. Immediately dry it with Ross lens tissue. If you wash it any other way or dry it with anything other than the Ross lens tissue, the film will not come off the glass.

Coat the slide immediately and let the film dry. Score the edges of the film so that it will easily detach from the glass and float it onto the water surface. Evaluate the film thickness and adjust the formvar concentration by adding chloroform to the solution.

I know there are many versions of this method that work. However, this is the only method I have found that is completely oblivious to the weather (still sunny in LA) and has been reproduced by many people.
in my experience, the formvar dissolved in choroform does not seem to have the short shelf life I read about recently. I have used the same solution for years, topping it up with chloroform to keep it at a concentration that will produce thin films.

Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


GUIDO LŸšND wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear readers,
} could anyone give me some hints or tips how I can separate a formvarfilm
} (0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
} help at all. It works better with non-washed slides, but then I get an
} uneven and dirty surface of the film.
} I appreciate every kind of support.
} Best regards
}
} Guido Luond
} Institute of Oral Microbiology and
} General Immunology
} Univ. of Zurich
} Plattenstr. 11
} CH-8028 Zurich, Switzerland
} E-mail: lueoend-at-zzmk.unizh.ch
}
}
}
}
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} Subject: TEM Formvar Film Cast on Glass
} From: GUIDO L=?ISO-8859-1?B?/PY=?=ND {lueoend-at-zzmk.unizh.ch}
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Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Feb 11 18:30:19 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 11 Feb 2000 14:01:04 -0500
Subject: metal pipe- inside surface

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hi all-

just got a request to view surface structure of the inside of a 12" metal
pipe. its too big for my chamber and they'd prefer a non-destructive
technique. will replication of the surface with acetate/acetone and SEM
work. i've never tried this before so any pointers would be welcome...

thx!
brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Fri Feb 11 18:30:26 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 11 Feb 2000 16:55:15 -0500
Subject: Fluorescent dyes -Clueless in Pittsburgh

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Laurie,
Frieda Carson's book about histologic technique is the "gold standard for
histotechs right now. It is available at Amazon.com and Barnes and Noble's
website, and can be special ordered at the major bookstores, as it is
currently in print. (Unfortunately I am at home and do not have the actual
title in front of me)
Wanda Shotsberger
----- Original Message -----
} From: Laurie Wallin {lwallin-at-ucsd.edu}
To: {microscopy-at-sparc5.Microscopy.Com}
Sent: Thursday, February 10, 2000 11:03 AM


I was asked a couple of questions about fluorescent microscopy and I didn't
have a clue. Are all the dyes used with UV or are there other wavelengths
that can fluoresce them? Are the dyes used dangerous/hazardous? Are they
transparent to visible? Are they expensive? Can you get them in quantity?

-Clueless in Pittsburgh


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



From daemon Fri Feb 11 19:43:36 2000



From: Jeff Allbright :      jeff.allbright-at-usa.net
Date: Fri, 11 Feb 2000 16:04:05 -0600
Subject: looking for ETEC Company

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I knew a very good, reputable company in the Southern California region that
serviced ETEC SEMs. The name was Scan Service in California, Earl Weltmer
714 area code.

- Jeff

-----Original Message-----
} From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER
[mailto:microsc-at-fi.uner.edu.ar]
Sent: Wednesday, February 09, 2000 5:10 AM
To: microscopy-at-sparc5.microscopy.com


Hello .....
we have an old ETEC Omniscan SEM, and we are searching for ETEC company or
any
people that can supply manuals ....

any help is welcome

best regards


===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================




From daemon Fri Feb 11 18:30:33 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 1/4/80 8:23 AM
Subject: questions for MSA members

Contents Retrieved from Microscopy Listserver Archives
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In regards to the diamond knives, both companies make an excellent
product and both provide good service. Because of price, the last 6
knives I've bought have been from Microstar (two well-used but dull
Diatome knives have been traded during these transactions) . All six
knives have been more than suitable for the intended purpose. I have
no commercial interest in either company.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


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A colleague would like your responses to these questions. I believe that I
say something on the first question about a year ago and apologize for
asking again.


} Here's what I/we need to know.
}
} 1) Are Microstar diamond knives equal to Diatome. We have an offer that
} will save us about $1200 on three knives ( one 3mm ultra with 45 degree
} angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).
}
}
} 2) Is the power controller feature on the Pelco Microwave Tissue processor
} (model #3451) worth it? (costs about $1300 additional compared to the
} Pelco 3450 without the power controller). I plan to do immunolocalization
} work, but am not sure this power controller feature is necessary or worth
} the cost.
}
} Casey
}
}
}
Thanks for your time
}
Marty Reed
Equipment Technician
Biology Department
Humboldt State University
Arcata CA 95521
707-826-3234
707-826-3201 FAX
mmr7001-at-axe.humboldt.edu





From daemon Fri Feb 11 18:30:36 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: 2/11/00 1:01 PM
Subject: metal pipe- inside surface

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Date sent: Fri, 11 Feb 2000 14:01:04 -0500
To: Microscopy-at-sparc5.Microscopy.Com
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}


Depends on the detail needed, but acetate replication should work for almost
all
situations. Of course, everything you see will be inside out... Often,
selecting inverse video helps one perceive thing properly. If the pipe is
dirty or corroded, you will likely pick up some deposit with the replica.
This
can be distracting, but can also be helpful if x-ray analysis is required...
Woody White
McDermott Technology
My place: http://www.geocities.com/capecanaveral/3722

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hi all-

just got a request to view surface structure of the inside of a 12" metal
pipe. its too big for my chamber and they'd prefer a non-destructive
technique. will replication of the surface with acetate/acetone and SEM
work. i've never tried this before so any pointers would be welcome...

thx!
brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Fri Feb 11 18:30:38 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 11 Feb 2000 18:23:11 -0500
Subject: PGT IMIX system module

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If any one has a PGT IMIX X-ray Analysis/Imaging system 4-40 (SUN Sparc)
that is in storage, or not in use, I would be interested in acquiring its
Image Signal Processor ISP module (Part# MO328D-00).



*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Sat Feb 12 03:29:53 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Fri, 11 Feb 2000 19:12:34 -0600
Subject: TEM LKB control Unit

Contents Retrieved from Microscopy Listserver Archives
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Dear Brian

Stop destroying your pipe. With our large chamber SEM we can analyse your
pipe up to a length of 35 inches and a diameter of 28 inches. Maximum weight
of the sample should not exceed 500 pounds.

Because of the positionable electron gun it is easy to observe the inside
surface.

Best regards
Martin Klein

Disclaimer: VisiTec is the manufacturer of large chamber SEMs. And we like
large samples.

----------------------------------------------------------------------------
---
VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen
Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: info-at-visitec-em.de
WWW: http://www.visitec-em.de

----- Original Message -----
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, February 11, 2000 8:01 PM


I am in desperate need of a control unit for a LKB ultratome III, since
mine is beyond repair.
I would appreciate if anyone coud sell me an unused unit from old
apparatus, even if it does not work, I might
be able to repair mine with the pieces.
Please reply to the e-mail below

Thanks

Dr. A.P. Alves de Matos
Dental Medical School
Lisbon University
apmatos-at-ip.pt




From daemon Sat Feb 12 03:29:45 2000



From: Brian Robertson :      brian.robertson-at-materials.oxford.ac.uk
Date: Fri, 11 Feb 2000 19:20:10 -0600
Subject: ELECTRON MICROSCOPY MATERIALS SPECIALIST

Contents Retrieved from Microscopy Listserver Archives
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ELECTRON MICROSCOPY MATERIALS SPECIALIST
UNL Center for Materials Research and Analysis

Analyze/characterize materials using electron microscopy, materials
preparation and computer instrumentation. Supervise/train students,
faculty and visiting researchers utilizing these methods. Bachelor's
with major in physical science, engineering or related field plus three
years experience in the operation, repair or design of electron
microscopes or other scientific instrumentation. Master's preferred.
Must have excellent computer and interpersonal/communication skills.
Knowledge of x-ray diffraction, TEM/SEM principles and sample
preparation experience preferred. Excellent benefits. Submit cover
letter, resume and names, addresses and telephone numbers of three
professional references to Professor David J. Sellmyer, 112 Brace Lab,
Lincoln, NE 68588-0113. Review of resumes will begin March 1. Position
will remain open until a suitable candidate is found. UNL is committed
to AA/EEO and ADA/504. If you require accommodation, please call (402)
472-8762.






From daemon Sat Feb 12 03:29:39 2000



From: michael baxter :      mykkb-at-juno.com
Date: Fri, 11 Feb 2000 19:21:23 -0600
Subject: Re: TEM-Formvar on Glass Slides

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Try a different brand of slide. Corning is the only brand we use in our EM
Class. There must be different kinds of surfactants used on them. Some
other brands of slides just don't release the formvar.
We usually wipe them off thoroughly with a Kimwipe only. Dip the slide
into the formvar and let dry. But don't dry for too long. Try using a
diamond tipped (or carbide) pen to score a "rectangle" on the slide.
Slowly insert the slide at about a 30 degree angle into the water. Wait
until you see the formvar start to release before you continue inserting
into the water.
This method usually works in our lab and for students.

Mike Baxter
Lehman College
Bronx, NY




From daemon Sun Feb 13 00:40:32 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 12 Feb 2000 01:13:04 -0800 (PST)
Subject: Re: wanted: microtome

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Dear Linda,
I have a DuPont Sorvall MT2B or a Porter Blum MT1 I would be interested in
selling if the price is right. If you are interested, let me know via my
email address: tiekotte-at-up.edu.
Cheers!
Ken
-----------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N. Willamette Blvd.
Portland, OR 97303

On Mon, 27 Aug 1956, Linda Chicoine wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone in the Connecticut area have an old microtome they would
} like to sell? I would be interested in anything from an LKB to RMC to
} Reichert/ and Leica. Please contact me by email. Thanks. Linda
} Chicoine
}
}
}



From daemon Sun Feb 13 00:40:33 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 12 Feb 2000 03:32:42 -0600
Subject: SEM pipe replication

Contents Retrieved from Microscopy Listserver Archives
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} To: MICROSCOPY BB {Microscopy-at-sparc5.microscopy.com}
} Subject: SEM pipe replication
} Date: Sat, 12 Feb 2000 10:44:32 -0500
} From: "Garber, Charles A." {cgarber-at-2spi.com}
} X-Mailer: E-Mail Connection v3.1a
} Content-Length: 3106

}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Brian McIntyre wrote:
} =========================================
} just got a request to view surface structure of the inside of a 12" metal
} pipe. its too big for my chamber and they'd prefer a non-destructive
} technique. will replication of the surface with acetate/acetone and SEM
} work. i've never tried this before so any pointers would be welcome...
} ==========================================
} You did not indicate how far into the pipe you want to do your looking,
but
} cellulose acetate is certainly one approach. The biggest problem you are
} going to have is to balance the need to do this all "nondestructively" vs.
} the need to see what might be underneath material covering the top surface

} (after all, it is the metal substrate that you really want to see). In
} other words, if the acetone should be a solvent for the contaminating
} material, (or if it lifts off metal oxide or other aspects of a scale) you
} will not be doing this very non-destructively. One technique is to make
} repetitive replicas on the same area, each additional one taking off more
of
} the contaminating material, in order to get to the bare metal surface.
But
} this might not be considered, by a court of law, to be nondestructive.
And
} if you are under an order to look at it nondestructively, this could
result
} in a problem with the court.
}
} When we have been faced with this dilemma, in order to first document what
} was there originally, we like to use our SPI "Wet Replica" kit, it is a
} silicone based system, and is described on our website. Certain dental
} replication systems such as "Sil-Flow" might work as well, but in general,
} for this kind of work, we believe our own kit is better. The silicone
resin
} in general won't remove surface contamination or scale. The drawback of
this
} technique is that after about 800X in the SEM you start to see "structure"
} from the replication system itself. A "plus" however is that you can make
a
} "replica of the replica", doing your SEM viewing on a positive, which does
} look just like the original inside surface.
}
} Once you have documented the state of the inside surface that way, you
} should be able to get permission to try the cellulose acetate replication
} method. There is additional information on the SPI Supplies website
relative
} to cellulose acetate replication methods.
}
} Disclaimer: SPI Supplies offers both cellulose acetate replicating tapes
} and sheets as well as the silicone based SPI "Wet Replica Kit" for use in
} electron microscopy applications.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
}
}
}
}




From daemon Sun Feb 13 00:40:40 2000



From: Dagmar Preusse :      Preusse-at-chemie.uni-bremen.de
Date: Sat, 12 Feb 2000 12:54:22 +0100
Subject: permanent staining of LR-White embedded root sections

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Hi,
I made an embedding of the endodermis of an iris root with LR-Wihte and
would like to make ultrathin sections and stain them. My problem is how
to stain the sections and how to receive permantly stained sections. Is
there anyone who has experiences with the staining of suberin in the 3rd
endodermis? Please inform me about the handling.
Thank you so much
Dagmar Preusse



From daemon Sun Feb 13 00:40:43 2000



From: Wright, Cheryl W :      CWright-at-Sikorsky.com
Date: Sat, 12 Feb 2000 08:25:19 -0500
Subject: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I run a 1830 Amray S.E.M. and work with failed aircraft components. From
time to time I am asked to replicate a surface using the acetate tape &
acetone method. The coating I use is a combination of Au and Pd. My
problem is that when I have to stay in one particular area of the replica,
the beam leaves a nice little square embedded into the surface of the
replica. I try to rapidly focus in full field instead of partial field, and
use between 6 - 10Kv. Does anyone have any advise for me on how to avoid
the embedded square?

Thanks, Cheryl



From daemon Sun Feb 13 00:40:51 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Sat, 12 Feb 2000 09:40:28 -0500
Subject: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
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Hi

It is the total current that you put into the specimen that is causing the
material to erode.

If you use a smaller spot size, smaller final aperture or smaller emission
current you should be better off? I do not know how good your SEM is at
low kV but if it was me I would use 2kV on this type of specimen; it will
be very sensitive even though it is coated.

To run happily at 2kV you need a filament to cathode distance that will
give you at least 30uA with your normal bias settings. If you do a good
deal of work on this type of specimen I would be inclined to lift the anode
by 5mm also. This will make the gun more efficient and make the job
easier.

Good luck

Steve Chapman
Protrain for Electron Microscopy Courses World Wide
E-mail - protrain-at-emcourses.com
Web Site - www.emcourses.com



From daemon Sun Feb 13 00:41:00 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sat, 12 Feb 2000 13:32:41 -0600
Subject: [LM] replication

Contents Retrieved from Microscopy Listserver Archives
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I have been trying to develop a styrene replica
fluid so I can use polorized light.So far the
styrene clumps ups in little islands insted of
making a nice film. I am using acetone as
a solvent

Has anyone had any luck with this?

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Sun Feb 13 16:26:42 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Sun, 13 Feb 2000 01:02:53 -0600
Subject: Re: questions for MSA members

Contents Retrieved from Microscopy Listserver Archives
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{fontfamily} {param} Monaco {/param} Marty,

I can address #2 with some confidence.  We have been using the
Pelco

Microwave Tissue processor (model #3451) for almost 2 years with the
power

controller and have found it essential for optimizing the procedures we
use

for antigen retrieval and immunolocalization at both the light and EM
level.

By being able to control the power you have much better control of the

"microwave effect" relative to heating. I would be happy to discuss
the

details with you directly.

Cheers,

Mark

****************************************************

Mark A. Sanders
           University
of Minnesota

Program Director
          Twin Cities
Campus

Imaging Center
            St.
Paul, MN  55108

23-35 Snyder Hall
         ph:
 612-624-3454

1475 Gortner Ave.
         fax:
612-624-1799

{/fontfamily} http://biosci.umn.edu/imagingcenter {fontfamily} {param} Monaco {/param}

President: Minnesota Microscopy Society

{/fontfamily} http://resolution.umn.edu/MMS/ {fontfamily} {param} Monaco {/param}



----------

} From: Marty Reed { {mmr7001-at-humboldt.edu}

} To: Microscopy-at-sparc5.Microscopy.Com

} Subject: questions for MSA members

} Date: Fri, Jan 4, 1980, 11:23 AM

}


}
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

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ListServer-at-MSA.Microscopy.Com

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}
-----------------------------------------------------------------------.

}

}

} A colleague would like your responses to these questions.  I
believe that I

} say something on the first question about a year ago and apologize
for

} asking again.

}

}

} }        Here's what I/we need to
know.

} }

} } 1)  Are Microstar diamond knives equal to Diatome.  We have
an offer that

} } will save us about $1200 on three knives ( one 3mm ultra with 45
degree

} } angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).

} }

} }

} } 2) Is the power controller feature on the Pelco Microwave Tissue
processor

} } (model #3451) worth it?  (costs about $1300 additional compared
to the

} } Pelco 3450 without the power controller).  I plan to do
immunolocalization

} } work, but am not sure this power controller feature is necessary or
worth

} } the cost.

} }

} } Casey

} }

} }

} }

} Thanks for your time

} }

} Marty Reed

} Equipment Technician

} Biology Department

} Humboldt State University

} Arcata CA 95521

} 707-826-3234

} 707-826-3201 FAX

} mmr7001-at-axe.humboldt.edu

}

}

{/fontfamily} Cheers, Mark
**************************************************** Mark A. Sanders
           University
of Minnesota Program Director
          Twin Cities
Campus Imaging Center
            St.
Paul, MN  55108 23-35 Snyder Hall
         ph:
 612-624-3454 1475 Gortner Ave.
         fax: 612-624-1799
http://biosci.umn.edu/imagingcenter President: Minnesota Microscopy
Society http://resolution.umn.edu/MMS/



{/x-rich}



From daemon Sun Feb 13 16:26:55 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Sun, 13 Feb 2000 12:02:10 -0600 (CST)
Subject: Microscopy and Microanalysis 2000

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13 Feb 2000 09:14:55 PST
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------- Start of forwarded message -------

Rules, FAQ Docs - Microscopy ListServer
To: NewSub-at-sparc5.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-sparc5.Microscopy.Com}

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To: NewSub-at-MSA.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}


Here is a final reminder and IMPORTANT CORRECTION for the Call for Papers for
Microscopy and Microanalysis 2000.

The title for symposium #05 in the Call for Papers was listed incorrectly. The
correct title should be "Phase Transitions" (If you check the symposium titles
on the electronic data submission forms it is listed correctly). X-ray
microanalysis papers should be directed to symposia 25 and 26.

The deadline for papers is Tuesday February 15, which is almost upon us.

Stuart McKernan (Program Chair)




__________________
Stuart McKernan stuartm-at-tc.umn.edu
Director Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368



From daemon Sun Feb 13 20:38:21 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 14 Feb 2000 12:29:53 +1100
Subject: metal pipe- inside surface

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} X-Sender: mcintyre-at-optics.rochester.edu
} Date: Fri, 11 Feb 2000 14:01:04 -0500
} To: Microscopy-at-sparc5.Microscopy.Com
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}
} Subject: metal pipe- inside surface
} Errors-to: Microscopy-request-at-sparc5.Microscopy.Com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The best way to make replicas is to use dental casting compound.

It has the ability to replicate very fine detail and accepts even damp
surfaces.

We used "Kerr Extrude Wash" (two part polyvinylsiloxane impression
material) to replicate (damp) tuna fish skin.

Once cured, we made epoxy positive casts from the negative casts, then
mounted, gold coated etc. for regular SEM.

Your local dental supply house will have this.

Kerr USA is at 28200 Wick Road, Romulus MI 48174-2600


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales Sydney, Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Sun Feb 13 23:08:57 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Mon, 14 Feb 2000 09:24:08 +0000 (GMT Standard Time)
Subject: Re: [LM] replication

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Dear Malcolm,

You could also consider Epson Expression 1600 Pro. There is a review at the
PC Magazine site:

http://www.zdnet.com/pcmag/stories/firstlooks/0,6763,2430926,00.html

True optical resolution 1600x3200. Dynamic range 3.3
Although it is not from a scientific point of view the authors claim that
this is the best scanner for the price they've ever seen.

Good luck,

Rado

Disclaimer: I'm not related to Epson company in any way.

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
To: Microscopy (MSA) {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 10, 2000 8:26 PM



On Sat, 12 Feb 2000, Gordon Couger wrote:

} I have been trying to develop a styrene replica
} fluid so I can use polarized light.So far the
} styrene clumps up in little islands instead of
} making a nice film. I am using acetone as
} a solvent

Polystyrene is only partially soluble in acetone, so the low MW goes into
solution while the high MW forms clumps or whatever. BUTANONE (MEK)
should work fine, but you'll have to give it perhaps 4 times the drying
time you'd need with acetone. For samples that are sensitive to polar
solvents such as these, you can also use toluene, but that takes an even
longer drying time.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Mon Feb 14 14:21:57 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Mon, 14 Feb 2000 09:35:52 +0000 (GMT Standard Time)
Subject: Re: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
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On Sat, 12 Feb 2000, Wright, Cheryl W wrote:

} I run a 1830 Amray S.E.M. and work with failed aircraft components. From
} time to time I am asked to replicate a surface using the acetate tape &
} acetone method. The coating I use is a combination of Au and Pd. My
} problem is that when I have to stay in one particular area of the replica,
} the beam leaves a nice little square embedded into the surface of the
} replica. I try to rapidly focus in full field instead of partial field, and
} use between 6 - 10Kv. Does anyone have any advise for me on how to avoid
} the embedded square?

Polystyrene MIGHT be a better material to use than cellulose acetate,
which tends to chemically self-destruct under electron beams: in fact,
when there is acetate remaining on replicas for TEM I sometimes burn it
off SLOWLY with the electron beam (too fast and you leave carbonaceous
stuff). The aromatic nature of PS also allows it to take up more energy.

You could replicate with PS, as follows:

Make a solution of PS in butanone (MEK) in a glass Petri dish. Allow to
evaporate slowly (if you can fume it off from a closed dish in an oven at
about 50^C, that's better). This film can then either be cast onto the
component with butanone, or you can melt-form it above the glass
transition of PS, say about 150^C. Don't use PS windows directly from
envelopes, because the films contain a lot of built-in-strain.

I'm not sure of it's performance in SEM, because I've used it for TEM
replication of acetone-sensitive specimens.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Mon Feb 14 14:22:01 2000



From: MARK GERMANI :      mmrinc-at-wwa.com
Date: Mon, 14 Feb 00 08:15:48 -0600
Subject: Pittcon Short Course

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} From: "Walck. Scott D." {walck-at-ppg.com}

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So your companyÕs SEM operator just retired. You have used light microscopes for years and they have asked you to run the SEM also. So now what do you do? The SEM manuals are long gone and do you really want to crack open that 800 page SEM/x-ray microanalysis textbook?

DonÕt worry, consider taking Electron Microscopy and Microanalysis a one-day short course to be held on Sunday, March 12, at PITTCON 2000 in New Orleans. The course is an introduction to SEM, TEM and x-ray microanalysis and is designed for analytical chemists and others who need to use these techniques for industrial problem-solving and product research and development.

For further information and online registration visit www.pittcon.org (Course #2029) or contact me offline.

Mark S. Germani, Ph.D.
MicroMaterials Research, Inc.
136 Shore Drive
Suite 200
Burr Ridge, IL 60521

(630) 325-8170
(630) 325-8178 (fax)

mmrinc-at-wwa.com



From daemon Mon Feb 14 14:22:21 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 14 Feb 2000 14:46:36 +0000 (GMT Standard Time)
Subject: Re: TEM LKB control Unit

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Electron Optical Services Ltd. make a replacement control
box. Their address is given below.

Dave



52 Higher Road,
Urmston,
Manchester,
M41 9AP
England
UK



Dave
On Fri, 11 Feb 2000 19:12:34 -0600 "A.P. Alves de Matos"
{apmatos-at-ip.pt} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I am in desperate need of a control unit for a LKB ultratome III, since
} mine is beyond repair.
} I would appreciate if anyone coud sell me an unused unit from old
} apparatus, even if it does not work, I might
} be able to repair mine with the pieces.
} Please reply to the e-mail below
}
} Thanks
}
} Dr. A.P. Alves de Matos
} Dental Medical School
} Lisbon University
} apmatos-at-ip.pt
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Feb 14 14:22:29 2000



From: Orth99-at-aol.com
Date: Mon, 14 Feb 2000 10:40:43 EST
Subject: Looking at the inside of metal pipes...

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How often does this community need to look at the inside of metal pipes or
pipes in general?

And, what is the typical surface roughness you are dealing with, i.e. would
an instrument with 100 um to 200 um of Z range be able to handle it?

And, what is the typical x,y range, i.e. would an instrument with 2 mm image
field be acceptable?

Just wondering. Thanks for any input you are willing to share.


Carol Rabke, Ph.D.
Surface Metrology Marketing & Applications Consultant
716-425-0976



From daemon Mon Feb 14 14:22:30 2000



From: Yvonne N“mcov‡ :      ynemcova-at-natur.cuni.cz
Date: Mon, 14 Feb 2000 16:53:33 +0100
Subject: embedding protocols

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopy Listserver members,
can someone provide me:
- a detailed protocol for embedding Chlamydomonas into the Lowicryl
Resin K4M (fixation media, desiccation solutions, times and
concentrations
that are suitable for Chlamydomonas)
- a fixation protocol for cryosectioning of Chlamydomonas

Thank you very much,
Yvonne Nemcova

--
*****************************************
Mgr. Yvonne Nemcova
Department of Botany, Charles University, Prague
Benatska 2. 128 01 Praha 2, Czech Republic
tel: 00-420-2-21953127, fax: 00-420-2-21953125
e-mail address: ynemcova-at-natur.cuni.cz
*****************************************




From daemon Mon Feb 14 14:22:59 2000



From: Virginie Serin :      serin-at-cemes.fr
Date: Mon, 14 Feb 2000 17:53:50 +0100
Subject: Re: EELS database, a new tool to play with

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott,


Thank you for the information on the MSA/MAS format that you sent me
some weeks ago.


The MSA/MAS spectra file format is now implemented in the EELS and XAS
database, therefore the spectra can be downloaded in this format.
Please let me know if you have any other problems. Any more suggestions
?

Thank you for your help.


Best regards


Virginie









{center} -----------------------------------------------


{fontfamily} {param} New_York {/param} {bigger} {bigger} Virginie Serin

{/bigger} {/bigger} {/fontfamily} {fontfamily} {param} Geneva {/param} CEMES-CNRS,
29 Rue J. Marvig, BP 4347,

31 055 Toulouse Cedex 4, France

TŽl: 33 (0)5 62 25 78 67, Fax : 33 (0)5 62 25 79 99,

e-mail: serin-at-cemes.fr

http://www.cemes.fr


{/fontfamily} EELS and X ray database : http://www.cemes.fr/~eelsdb/


-----------------------------------------------

{/center}


{/x-rich}



From daemon Mon Feb 14 14:23:27 2000



From: tea meulia :      meulia.1-at-osu.edu
Date: Mon, 14 Feb 2000 14:54:32 -0500 (EST)
Subject: Edwards Vacuum Evaporator

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We are having an Edwards Vacuum Evaporator model E12E2 with a control unit
EVM052 in excess. This instrument has not been used for several year and it
may need some maintanance work. If you are interested please contact Tea
Meulia (meulia.1-at-osu.edu) or Pat Ashbaugh (ashbaugh.11-at-osu.edu).


Tea Meulia



Tea Meulia
Research Scientist and Head
Molecular and Cellular Imaging Center
OSU/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: (330) 263'3828
fax: (330) 202'3563

http://www.oardc.ohio-state.edu/mcic




From daemon Mon Feb 14 17:03:40 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Mon, 14 Feb 2000 15:24:38 -0600
Subject: Re: [LM] replication

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all that responded. I have a lot better handle on how to
do it. I will report back my results.

Thanks to all for providing a great source of infomation.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "Robert H. Olley" {r.h.olley-at-reading.ac.uk}
} On Sat, 12 Feb 2000, Gordon Couger wrote:
}
} } I have been trying to develop a styrene replica
} } fluid so I can use polarized light.So far the
} } styrene clumps up in little islands instead of
} } making a nice film. I am using acetone as
} } a solvent
}
} Polystyrene is only partially soluble in acetone, so the low MW goes into
} solution while the high MW forms clumps or whatever. BUTANONE (MEK)
} should work fine, but you'll have to give it perhaps 4 times the drying
} time you'd need with acetone. For samples that are sensitive to polar
} solvents such as these, you can also use toluene, but that takes an even
} longer drying time.
}





From daemon Mon Feb 14 20:36:02 2000



From: Tom Reese :      treese-at-mbl.edu
Date: Mon, 14 Feb 2000 17:56:31 -0600
Subject: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
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Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
but they claim it is much better than the much cheaper models many of
us know and love. Would anyone know whether this is true of just
hype-hard to tell from the published specs...? Thanks...Tom Reese



From daemon Tue Feb 15 16:18:33 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 15 Feb 2000 00:05:37 -0800 (PST)
Subject: Re: [LM] replication

Contents Retrieved from Microscopy Listserver Archives
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Dear Gordon,
Try xylene. It works quite nicely as a mounting medium of a differenting
refractive index from that of say, canada balsam or Permount. Email me if
you have questions. Good luck!
Cheers,
Ken

-------------
Ken Tiekotter
Dept. of Biology
The Univerisity of Portland
5000 N. Willamette Blvd.
Portland, OR 97303


On Sat, 12 Feb 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have been trying to develop a styrene replica
} fluid so I can use polorized light.So far the
} styrene clumps ups in little islands insted of
} making a nice film. I am using acetone as
} a solvent
}
} Has anyone had any luck with this?
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}
}
}
}



From daemon Tue Feb 15 16:18:36 2000



From: Kuusisto, Ari :      Ari.Kuusisto-at-wallac.fi
Date: Tue, 15 Feb 2000 10:34:16 +0200
Subject: RE: autofluorescence quenching

Contents Retrieved from Microscopy Listserver Archives
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Corazon ,

You wrote
"I am working with archival specimens of formalin-fixed paraffin embedded
tissues and I am getting tremendous autofluorescence. I looked at tissue
sections after the sections have been de-waxed using either FITC or
rhodamine filter sets and red cells and connective tissues are brightly
fluorescent. Is there a way of suppressing the autofluorescence and still
retain reactivity of tissue to antibodies? I would appreciate any comments
or suggestions."

It is an unfortunate fact that most fixatives cause high autofluorescence
making the studies with conventional fluorescence microscope hard. The
disturbance of autofluorescence can be avoided by using time-resolved
fluorescence microscope and special labels (See the following articles:
VŠisŠlŠ M, SevŽus L, Kuusisto A, Harju R, and Soini
E: Time- resolved fluorescence microscopy: Elimination of autofluorescence
in tissue specimens for Image cytometry with fluorescent labelled probes.
Micron and Microscopica Acta 21; 3, (1990).

SevŽus L, Kuusisto A, VŠisŠlŠ M, HemmilŠ I., and
Soini E: Lanthanide
chelates as labels in in situ hybridization and
immunohistochemistry,
Micron and Microscopica Acta 21; 3, (1990).

SevŽus L, VŠisŠlŠ M, SyrjŠnen S, Sandberg M,
Kuusisto A, Harju R, Salo J, HemmilŠ I, Kojola H, and Soini E: Time-Resolved
Fluorescence Imaging of Europium Chelate Label in Immunohistochemistry and
In Situ Hybridization, Cytometry 13; 329, (1992).

SevŽus L, Kuusisto A, VŠisŠlŠ M, HemmilŠ I., Kojola
H., Roomans G. M. and Soini E.: Use of Fluorescent Europium Chelates as
Labels in Microscopy Allows Glutaraldefyde Fixation and Permanent Mounting
and Leads to Reduced Autofluorescence and Good Long-Time Stability,
Microscopy Research and Technique 27; 00, (1994).

I hope this information helps you.




Ari Kuusisto, Research Physicist

Phone: +358 2 267 8508 (direct)
+358 2 267 8111 (switchboard)
Fax: +358 2 267 8380
e-mail: Ari.Kuusisto-at-wallac.fi {mailto:Ari.Kuusisto-at-wallac.fi}
Address: Wallac Oy
PO Box 10
FIN-20101 Turku, Finland
Homepage: www.wallac.fi {http://www.wallac.fi}




From daemon Tue Feb 15 16:19:06 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Tue, 15 Feb 2000 14:02:33 +0000
Subject: Re: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
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I saw the Epson web spec and it looks quite impressive - especially as
they seem to be quoting speeds for real picture printing, not 5%
coverage or text.

My only concern is that Epson now market two printers with "10 years
light fastness" (the Epson Stylus photo 870 - A4 and 1270 A3) so you
have to wonder which is better for e.m. prints.

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

---------------------------------------------------------

Tom Reese wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
} but they claim it is much better than the much cheaper models many of
} us know and love. Would anyone know whether this is true of just
} hype-hard to tell from the published specs...? Thanks...Tom Reese



From daemon Tue Feb 15 16:19:04 2000



From: marienhoff.visitec-at-t-online.de (Marienhoff)
Date: Tue, 15 Feb 2000 15:10:14 +0100
Subject: Re: Looking at the inside of metal pipes...

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Carol,

We were looking up to 70 mm deep inside cylinder bores and engine blocks
several times during the last months with our large-chamber SEM. Roughness
was typically very small (less than 10 µm), but for small diameter tubes
the positioning system needs large travels, tilts, and rotary axes to inspect
every interesting area.

Disclaimer: Visitec manufactures the large-chamber SEM.

Best regards
Peter
___________________________
Dr. Peter Marienhoff
VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen
Germany

Fon: +49-3881-790-47
Fax: +49-3881-790-48
email: pmarienhoff-at-visitec-em.de
http://www.visitec-em.de

"Orth99-at-aol.com"-at-sparc5.Microscopy.Com wrote:
} How often does this community need to look at the inside of metal pipes or
} pipes in general?
}
} And, what is the typical surface roughness you are dealing with, i.e. would
} an instrument with 100 um to 200 um of Z range be able to handle it?
}
} And, what is the typical x,y range, i.e. would an instrument with 2 mm image
} field be acceptable?
}
} Just wondering. Thanks for any input you are willing to share.
}
}
} Carol Rabke, Ph.D.
} Surface Metrology Marketing & Applications Consultant
} 716-425-0976
}



From daemon Tue Feb 15 16:19:08 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 15 Feb 2000 10:12:15 -0500
Subject: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
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The Epson 5000 is a printer/proofer using 6 color inkjet technology. The 6
color technology expands the available color gamut significantly. It is
available both with or without a Fiery RIP. The Fiery RIP is used for
Postscript processing of complex files from Quark, Pagemaker,
Illustrator,etc.
Without the Fiery box, an Epson software RIP is included. Print quality is
excellent although without the Fiery RIP, printing times can be
long,especially for 11x17 or larger prints. For more information see:
http://prographics.epson.com/products/stypro5000/index.html

Canon also has an excellent printer, the BJC-8500 that also uses 6 color
technology. The BJC-8500 also handles up to 13x19 paper with excellent
print quality. Canon utilizes a technology that optimizes ink drying and
water resistance. For more information see:
http://www.bjc8500.com/index2.html

Neither of these printers are speed demons in terms of page throughput, but
quality is excellent. I will be happy to answer any questions anyone may
have regarding output devices.

George Laing
National Graphic Supply

-----Original Message-----
} From: Tom Reese [mailto:treese-at-mbl.edu]
Sent: Monday, February 14, 2000 6:57 PM
To: Microscopy-at-sparc5.microscopy.com


Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
but they claim it is much better than the much cheaper models many of
us know and love. Would anyone know whether this is true of just
hype-hard to tell from the published specs...? Thanks...Tom Reese




From daemon Tue Feb 15 16:19:10 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 15 Feb 2000 09:25:24 -0600
Subject: RE: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
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I'd try, besides using low kV and beam current,
to increase substantially thickness of Au/Pd coating.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Wright, Cheryl W [mailto:CWright-at-Sikorsky.com]
} Sent: Saturday, February 12, 2000 7:25 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Photography of Coated Replicas
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I run a 1830 Amray S.E.M. and work with failed aircraft
} components. From
} time to time I am asked to replicate a surface using the
} acetate tape &
} acetone method. The coating I use is a combination of Au and Pd. My
} problem is that when I have to stay in one particular area of
} the replica,
} the beam leaves a nice little square embedded into the surface of the
} replica. I try to rapidly focus in full field instead of
} partial field, and
} use between 6 - 10Kv. Does anyone have any advise for me on
} how to avoid
} the embedded square?
}
} Thanks, Cheryl
}



From daemon Tue Feb 15 16:19:28 2000



From: Pearl Martin :      image-at-optonline.net
Date: Tue, 15 Feb 2000 11:37:14 -0500
Subject: Job: Sr. Metallographic Tech SEM/X-ray

Contents Retrieved from Microscopy Listserver Archives
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I am a technical recruiter. Currently, I am working on a search for one
of my Northeast clients for a
Senior Metallographic Technician:


Will perform metallographic evaluation of coatings including work with
SEM (Scanning Electron
Microscopy) and X-ray. Will be responsible for the metallurgy
laboratory, including organization of
work, and operation and calibration of equipment. Will write technical
and engineering reports. Will
prepare projects for the metallurgy laboratory. Will maintain
communication with customers.

AAS/BS plus fours years experience in a metallography laboratory.

Resumes should be sent by email, mail or fax. For best results, please
send emails as attached
Word.docs - I have Word 97.

Pearl Martin
Image Associates Inc.
5254 Merrick Road
Massapequa, NY 11758
Phone (516)798-3993
Fax (516)797-8703
Email: pearl-at-jobspot.com



From daemon Tue Feb 15 16:19:32 2000



From: Furdanowicz, Waldemar :      rwafu-at-bsco.com
Date: Tue, 15 Feb 2000 12:19:14 -0500
Subject: RE: questions for MSA members (i.e. diamond knives)

Contents Retrieved from Microscopy Listserver Archives
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With regard to diamond knives, I think it depends on what you're cutting and
what quality of section you require.

We cut steel and cross sections of coatings (some very hard and brittle) on
steel, and the only knife that approaches satisfactory result is Diatome 35
degree, sharpened to extra fine edge. We traded our big old Microstar knife
for a 2nd Diatome, because it couldn't make good, electron transparent
sections.

On the other hand, if your material cuts as well with a glass knife as with
a diamond but you don't want to bother making your own glass knives, then go
ahead and save some dollars.

(In my view, the same reasoning applies to the brand of ultramicrotome to
use. My company is very frugal, and there is always strong pressure to save
a dollar here and there, but we found that only Reichert/Leica can
consistently produce good samples in our demanding application; and the
higher initial cost has been more than offset by savings in time and effort,
and by the quality of results. (I.e., often the ability to just get any kind
of a result).)

Valdemar Furdanowicz, Ph.D.
Research Labs - G165
Bethlehem Steel
Bethlehem PA 18016

No interest in Microstar, Diatome, Leica; and this not reflect an opinion or
position of my employer....etc.

-----Original Message-----
From: Chuck Butterick [mailto:cbutte-at-ameripol.com]
Sent: Friday, February 11, 2000 5:11 PM
To: Microscopy-at-sparc5.Microscopy.Com;
mmr7001-at-humboldt.edu
Subject: Re: questions for MSA members


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of America
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-----------------------------------------------------------------------.


In regards to the diamond knives, both companies make
an excellent
product and both provide good service. Because of
price, the last 6
knives I've bought have been from Microstar (two
well-used but dull
Diatome knives have been traded during these
transactions) . All six
knives have been more than suitable for the intended
purpose. I have
no commercial interest in either company.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator
_________________________________
Subject: questions for MSA members
Author: Marty Reed {mmr7001-at-humboldt.edu} at INTERNET-MAIL
Date: 1/4/80 8:23 AM



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-----------------------------------------------------------------------.


A colleague would like your responses to these questions. I
believe that I
say something on the first question about a year ago and
apologize for
asking again.


} Here's what I/we need to know.
}
} 1) Are Microstar diamond knives equal to Diatome. We have
an offer that
} will save us about $1200 on three knives ( one 3mm ultra
with 45 degree
} angle, one 3mm ultra with 35 degree angle, one 10 mm histo
knife).
}
}
} 2) Is the power controller feature on the Pelco Microwave
Tissue processor
} (model #3451) worth it? (costs about $1300 additional
compared to the
} Pelco 3450 without the power controller). I plan to do
immunolocalization
} work, but am not sure this power controller feature is
necessary or worth
} the cost.
}
} Casey
}
}
}
Thanks for your time
}
Marty Reed
Equipment Technician
Biology Department
Humboldt State University
Arcata CA 95521
707-826-3234
707-826-3201 FAX
mmr7001-at-axe.humboldt.edu






From daemon Tue Feb 15 16:19:37 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 15 Feb 2000 09:47:15 -0800
Subject: deconvolution vendors

Contents Retrieved from Microscopy Listserver Archives
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We are considering the purchase of a separate deconvolution system to
augment our imaging lab's confocal system Any users or vendors with
information about such systems, please contact me offline.

thanks in advance

steve


Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/emfacility/

Chairman, Educational Outreach subcommittee
promoting access to microscopes
Microscopy Society of America http://www.msa.microscopy.com/




From daemon Tue Feb 15 16:19:39 2000



From: ERIC :      biology-at-ucla.edu
Date: Tue, 15 Feb 2000 10:37:36 -0800
Subject: Oven for curing blocks

Contents Retrieved from Microscopy Listserver Archives
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To the wealth of knowledge on the list,

We are in the market for a new oven for curing blocks.... Any
suggestions? This lab uses as a standard Eponate 12 as the epoxy resin of
choice....
The oven we currently have after being fixed several times does not see to
keep a constant temp.....




From daemon Tue Feb 15 16:19:48 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Tue, 15 Feb 2000 15:11:33 -0400
Subject: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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One of the members of this listserver group just asked me privately about
the fact that he had noticed that the reading of the thermocouple gauge on
his vacuum evaporator changed markedly when he jiggled the wires running
from the gauge tube to the gauge control unit. This is a matter that is
discussed in some detail on page 87 of my book 'Vacuum Methods in Electron
Microscopy' (see http://www.2spi.com/catalog/books/book48.html for a
description)

Actually, this is a rather common problem with thermocouple gauges which
all people using them should be aware of. It arises because the sensing
element in a thermocouple gauge is an arrangement of very fine wires that
form one or more thermocouple junctions. The output of these gauges is
thus the electrical potential produced by these junctions that is of the
order of only 10 or 15 millivolts DC. As a consequence, the signal detected
by the gauge controller can be strongly influenced by minor changes the
resistance of the electrical lines connecting the gauge to the controller.
Typically, unplugging one of the connectors in this line and reconnecting
it can change the resistance of the line sufficiently to cause a
significant change in the reading produced by the controller.

For this reason, considerable care must be exercised in installing and
maintaining thermocouple gauges if acceptable readings are to be obtained.
As a minimum, the same cables used in calibrating a gauge must subsequently
be used when it is put into operation, and the pins and sockets of the
plugs involved in the circuit must be kept clean and firmly connected.

Because of the low DC voltages that must be detected, the meters used in
the gauge circuit must be rather delicate and sensitive. We have
encountered situations when a static electric charge on the plastic window
of a meter affected the movement of the needle inside the meter enough to
prevent an accurate response. Coating the meter window with an anti-static
solution cured this situation.

As discussed in my book, Thermocouple gauges are rugged, inexpensive, and
very convenient for use in many applications, but they must be handled with
care if they are to give acceptable service.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Tue Feb 15 20:09:28 2000



From: Karen Kavanagh :      kavanagh-at-sfu.ca
Date: Tue, 15 Feb 2000 14:26:57 -0800 (PST)
Subject: TEM available Philips EM-300

Contents Retrieved from Microscopy Listserver Archives
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A working Philips EM 300 TEM is available for donation to
a non-profit organization.
Please contact Karen Kavanagh at
kavanagh-at-sfu.ca




From daemon Tue Feb 15 20:09:41 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Tue, 15 Feb 2000 18:02:57 -0600 (CST)
Subject: TEM - glutaraldehyde for perfusion

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Hello everyone,

A graduate student is getting ready for a rather large scale preparation
of rat brains for TEM using vascular perfusion. Luckily (since I'm a
botanist) her major professor has had experience with the procedure. He
doesn't know for sure whether he used EM or Biological grade
glutaraldehyde previously, but considering the large volume that will be
required, and the cost difference, I'm hoping that the Biological grade
will suffice.

I would appreciate advice from those of you that have experience with this
method of fixation.

Thank you in advance for any information.

Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Tue Feb 15 20:09:42 2000



From: JosŽ Ull‡n Serrano :      jullan-at-mixcoac.upmx.mx
Date: Tue, 15 Feb 2000 18:05:34 -0600
Subject: epifluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Colleagues:

I have attempted to purchase an epifluorescence nose from 'Carl Zeiss'
to fit the old "Universal" model scopes, but was dismayed to find out that
it is no longer available.
If anyone has one that is not being used and wishes to sell it, I would be
very interested.

Thanks

JOSE ULLAN SERRANO, Ph.D., M.D.
ÑÑÑÑÑÑÑÑÑÑÑÑÑÑÑÑ
E-mail: jullan-at-mixcoac.upmx.mx

Departamento de ANATOMIA
Escuela de MEDICINA
Universidad Panamericana
c/ Donatello, 59
Col. Insurgentes-Mixcoac
03920 MEXICO, D.F.
ÑÑÑÑÑÑÑÑÑÑÑÑÑÑÑ
http://www.mixcoac.upmx.mx
Telef. 55 98 33 02 / 55 63 13 43
FAX: 56 11 35 85



From daemon Wed Feb 16 00:57:58 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 15 Feb 2000 20:05:09 -0600
Subject: Re: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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Thermocouple gauges are more like gross indicators of vacuum. As a
resistive bridge, they are driven by constant current sources and the
bridge or sensor output is fed to a differential amplifier. This amplifier
has a gain and zero adjust potentiometer. If either of these are dirty
or defective, readings will be off. But if a thermocouple sensor is
exhibiting erratic readings, and it uses the standard vacuum tube socket
connector, I'd replace the detector right off the bat. They do eventually
fail. A $25 throw away isn't worth the hassle.

Most instruments, like SEMs, use the TC gauge to indicate that the mechanical
pump is working. Once the turbo pump spins up to } 80% RPM, the TC gauge
is rather useless. Better readings are from cold cathode gauges....which tend
not to fire very regularly. Hence, the optimum readings are from the ion pump
current.

At 01:11 PM 2/15/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 16 00:58:02 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 15 Feb 2000 20:13:52 -0800
Subject: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Heather

We perfuse 4% formaldehyde in 1x PBS only, than prepared the brain's slices
.3-.4 mm thick and fix them in a mixture 3-4% formaldehyde 2% GA (all - EM
grade) in 1x PBS. If you would like to add GA into perfusion solution - it
is only 0.1% - not a big deal. From another hand, I don't think Biological
grade GA will affects fixation quality. But, who knows, every time it is a
"game": if fixation is good - no problem, if - no, we will start to think
what's going on and "biological" grade may be a "case"... To avoid such
troubles I always used fresh GA and formaldehyde from non-opened ampoules.

Good luck, Sergey


} Date: Tue, 15 Feb 2000 18:02:57 -0600 (CST)
} From: Heather A Owen {owenha-at-csd.uwm.edu}
} Subject: TEM - glutaraldehyde for perfusion
} To: MSA Listserver {Microscopy-at-sparc5.Microscopy.Com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Feb 16 00:58:04 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 15 Feb 2000 21:20:05 -0800
Subject: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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Dear Wilbur

As for my knowledge (mostly from physic's course at 7th grade Russian
school) the thermocouple gauge has two "thermocouples": the probe itself
and reference thermocouple inside of gauge (separate "references" for each
type of probe, I believe). All together they generate enough response to
be easily measured. I agree that the major problem in your case is a bad
electrical contact in the connectors or even broken wire (it is well
possible, because some material uses for thermocouples are very fragile).
In my point of view, thermocouple gauges is very reliably and most accurate
devices and they do not need any special care.

I am using BARNANT-100 Thermocouple Gauge and REX-P96 Thermocouple
Controller with type "T" thermocouple sensors (copper/constantan, I
believe, but not sure) for many years without any problem. The sensor goes
into the vacuum chamber through regular 8-pin feed-thought. I was using
same as thermocouple material for screws to connect thermocouple's wires to
the feed-though wiring. For some reason, soldering does not work. I took
that screws from disassembled thermocouple connectors (looks like small
plugs) most suppliers sells for thermocouple probes (a dollar each). That
connectors should correspond to the thermocouple probe you are using.
Usually those connectors are color-coded. For instance connectors for my
type "T" probe was coded in blue. BARNANT-100 Thermocouple Gauge and
REX-P96 Controller performs self-calibration test every time you shut it ON
or disconnect/connect probe. During that calibration they "compensate"
some electrical abnormalities in the circuit like difference in the probe
length or as in my case the current deviation on the enter and end of the
feed-through. In theory, the "break" in thermocouple wiring on
feed-through should affect the gauge accuracy. In practice, I did not find
a difference between "solid" and with-feed-though probe up to 0.1oC
accuracy (which is sufficient for my needs). REX-P96 Thermocouple
Controller is just amazing. You may program complicated profiles for
cooling/heating cycles and it holds temperature pretty well. It has
special auto-calibration function to adjust heat rate depending from
parameters of your system. In practice it mean that your sample will not
overheated because of heater inertia. I am using that Controller in my
high-resolution shadowing device to control temperature from -150 to +50oC.

I have no interest in described products (only happy user) mostly because I
even don't know the names of real manufacturers. I remember it was
distributed by Cole-Parmer.

Sergey



} Date: Tue, 15 Feb 2000 15:11:33 -0400
} From: Wil Bigelow {bigelow-at-engin.umich.edu}
} Subject: Thermocouple gauges
} X-Sender: bigelow-at-srvr5.engin.umich.edu
} To: Microscopy Listserver {microscopy-at-sparc5.Microscopy.Com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Feb 16 16:21:13 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 16 Feb 2000 01:53:56 -0600
Subject: Re: [LM] replication Thanks!!

Contents Retrieved from Microscopy Listserver Archives
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MEK worked great. I broke up the clear plastic from a
CDROM case and made a thick solution by adding MEK.
cleaned a slide and spread a drop of the solution on the
slide and put a celery leaf on it I covered the whole thing
with saran warp and gently pressed another slide on it
to get good contact. Then removed the slide and Saran
wrap.

After drying about 30 minutes I pealed the leaf off and
took a look under a Nomarski phase scope. It was
the best image I have seen on the scope. The Nomarski
colors were extremely strong and I could get three bands
of color in some features/artifacts. The effect of the styrene
on the polarized light were not as strong as I had hoped for
but they do increase the contrast and Nomarski colors. It
will make lovely photos. Now if my color CCD camera would
just get here. Monochrome just won't do it justice.

I has been a long time since I looked at a leaf but the
stoma showed up just like the books show and I remember.
Individual cells were very clearly visible.

The film is extremely fragile and probably needs a little
plastizer added and probably go to a go to a multi coat
system if the film needs to be pealed from a surface and
mounted. with the first coats being thin with little or no
plastizier and the following coats being thicker and
carrying more plastisizer.

My thanks to all the helped on this. I will probably try
other solvents but MEK does not set off my asthma
like xylene and toluene do. So I will probably put up
with the slower drying times.

I was very impressed with the detail that was replicated.

Thanks
Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




} From: Robert H. Olley [mailto:r.h.olley-at-reading.ac.uk]
} On Sat, 12 Feb 2000, Gordon Couger wrote:
}
} } I have been trying to develop a styrene replica
} } fluid so I can use polarized light.So far the
} } styrene clumps up in little islands instead of
} } making a nice film. I am using acetone as
} } a solvent
}
} Polystyrene is only partially soluble in acetone, so the low MW goes into
} solution while the high MW forms clumps or whatever. BUTANONE (MEK)
} should work fine, but you'll have to give it perhaps 4 times the drying
} time you'd need with acetone. For samples that are sensitive to polar
} solvents such as these, you can also use toluene, but that takes an even
} longer drying time.





From daemon Wed Feb 16 16:21:19 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 16 Feb 2000 01:20:42 -0800 (PST)
Subject: Re: epifluorescence

Contents Retrieved from Microscopy Listserver Archives
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Dear Josi,
Try contacting John Oren at Vermont Optechs. I cannot tell you
immediately what his email or web site address is but his mail address is:
P.O. Box 69, Charlotte, VT 05445: Tel. (802) 425-2040; FAX. (802)
425-2074. Good luck!
-Ken


On Tue, 15 Feb 2000, Jos[ISO-8859-1] é Ullán Serrano wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Colleagues:
}
} I have attempted to purchase an epifluorescence nose from 'Carl Zeiss'
} to fit the old "Universal" model scopes, but was dismayed to find out that
} it is no longer available.
} If anyone has one that is not being used and wishes to sell it, I would be
} very interested.
}
} Thanks
}
} JOSE ULLAN SERRANO, Ph.D., M.D.
} ‹‹‹‹‹‹‹‹‹‹‹‹‹‹‹‹
} E-mail: jullan-at-mixcoac.upmx.mx
}
} Departamento de ANATOMIA
} Escuela de MEDICINA
} Universidad Panamericana
} c/ Donatello, 59
} Col. Insurgentes-Mixcoac
} 03920 MEXICO, D.F.
} ‹‹‹‹‹‹‹‹‹‹‹‹‹‹‹
} http://www.mixcoac.upmx.mx
} Telef. 55 98 33 02 / 55 63 13 43
} FAX: 56 11 35 85
}
}



From daemon Wed Feb 16 16:21:18 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Wed, 16 Feb 2000 10:28:42 +0100 (MET)
Subject: TEM, biology: Diatome knives

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I have heard recently that Diatome invent some coating of their diamond
knives that significantly improve the quality of cryosections (they appear
more smooth, less compressed, practically without folds). Is it true or
just usual story to increase sales?

Sincerely,
Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it




From daemon Wed Feb 16 16:21:28 2000



From: David_Bell-at-Millipore.com
Date: Wed, 16 Feb 2000 08:25:55 -0500
Subject: Re: RJ Lee Instruments: Many Thanks!

Contents Retrieved from Microscopy Listserver Archives
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Hello again,

I want to thank everyone who took the time to reply to me. Your emails were
very informative and helpful, and I passed them along to my coworker.

Thanks again,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Wed Feb 16 16:21:33 2000



From: rlvaughn-at-unmc.edu
Date: Wed, 16 Feb 2000 08:48:39 -0600
Subject: TEM repair services

Contents Retrieved from Microscopy Listserver Archives
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Speaking of thermocouples, I need to replace (probably) the T1 and T2
thermocouples in our OLD Denton evaporator. Where, other than Denton, can
I look for these?
I also need service work done on our old Ultracut (the model before the "E"
series). I refuse to work with Leica. Is there anyone in the "real"
midwest area?



From daemon Wed Feb 16 16:21:40 2000



From: Bede Willenbring :      Bede.Willenbring-at-HBFuller.com
Date: Wed, 16 Feb 2000 09:20:53 -0600
Subject: Re: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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I've also seen this type of behavior caused by a "cold" solder joint on one or more of the wires in the guage connector socket. Re-flowing the joints did wonders for stability.



============================================================
Bede Willenbring Phone: (651)236-5470
H.B. Fuller Company FAX: (651)236-5430
Research Chemist E-mail: Bede.Willenbring-at-HBFuller.com
P.O. Box 64683
St. Paul, MN 55164-0683



} } } Wil Bigelow {bigelow-at-engin.umich.edu} 02/15 1:11 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


One of the members of this listserver group just asked me privately about
the fact that he had noticed that the reading of the thermocouple gauge on
his vacuum evaporator changed markedly when he jiggled the wires running
from the gauge tube to the gauge control unit. This is a matter that is
discussed in some detail on page 87 of my book 'Vacuum Methods in Electron
Microscopy' (see http://www.2spi.com/catalog/books/book48.html for a
description)

Actually, this is a rather common problem with thermocouple gauges which
all people using them should be aware of. It arises because the sensing
element in a thermocouple gauge is an arrangement of very fine wires that
form one or more thermocouple junctions. The output of these gauges is
thus the electrical potential produced by these junctions that is of the
order of only 10 or 15 millivolts DC. As a consequence, the signal detected
by the gauge controller can be strongly influenced by minor changes the
resistance of the electrical lines connecting the gauge to the controller.
Typically, unplugging one of the connectors in this line and reconnecting
it can change the resistance of the line sufficiently to cause a
significant change in the reading produced by the controller.

For this reason, considerable care must be exercised in installing and
maintaining thermocouple gauges if acceptable readings are to be obtained.
As a minimum, the same cables used in calibrating a gauge must subsequently
be used when it is put into operation, and the pins and sockets of the
plugs involved in the circuit must be kept clean and firmly connected.

Because of the low DC voltages that must be detected, the meters used in
the gauge circuit must be rather delicate and sensitive. We have
encountered situations when a static electric charge on the plastic window
of a meter affected the movement of the needle inside the meter enough to
prevent an accurate response. Coating the meter window with an anti-static
solution cured this situation.

As discussed in my book, Thermocouple gauges are rugged, inexpensive, and
very convenient for use in many applications, but they must be handled with
care if they are to give acceptable service.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237






From daemon Wed Feb 16 16:21:44 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Wed, 16 Feb 2000 11:02:40 -0500
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Heckman wrote:

} Heather A Owen wrote:
}
} } Hello everyone,
} }
} } A graduate student is getting ready for a rather large scale preparation
} } of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} } botanist) her major professor has had experience with the procedure. He
} } doesn't know for sure whether he used EM or Biological grade
} } glutaraldehyde previously, but considering the large volume that will be
} } required, and the cost difference, I'm hoping that the Biological grade
} } will suffice.
} }
} } Heather,
}
} It's been a while but, back when I did such things, I had bouts of
} non-uniform fixation when using biological grade glutaraldehyde solutions
} even in plant tissues. However, the solutions had been kept much longer than
} I'd now use and there are other preparation technique changes that confound
} the determination of causality. In the cases where I've perfused small
} animals, I've used EM grade GA since, as expensive as it is, it's cheaper
} than repeating the experiment.
}
} However, I did a full systemic perfusion. One random thought ( I haven't
} tried it): What's the vascular volume of a rat brain? If you just need just
} the brain, I'd think a little creative clamping (really small hemostats?) in
} the thorax should let you reduce the volume needed to make even a large
} experiment affordable with "the good stuff".
}
} cheers and good luck,
} John
}

Heather:

I agree with John on both counts. We clamp the aorta (and anything eles that
gets in the way) high in the abdomen to avoid perfusing organs we are not
interested in. Saves time and money. Also we tilt the animal once perfusion is
underway so that the head is lower.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Feb 16 16:21:45 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 16 Feb 2000 08:21:17 -0800 (PST)
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had a lot of years' experience doing vascular perfusion of the CNS of
rodents (rat and mouse). We have used the EM Sciences Biological Grade
glutaraldehyde, 50%, as our primary source for perfusion without problems
related to fixation. However, we have not used straight glutaraldehyde, but
found that 2% paraformaldehyde + 1% glutaraldehyde in 0.1M cacodylate buffer
at pH 7.0 to 7.2 gave consistent results with excellent preservation of
structures, morphology and cytoplasmic and nuclear components. We have used
cacodylate primarily because we use uranyl acetate stain either in the
dehydration/processing protocol and/or on the thin sections. Use of
phosphate buffers invariably results in precipitate formation, even when
used only on sections. Another reason for the paraform/glut/cacodylate
combination is that we have studied the leakage of tracers (e.g. HRP,
microperoxidase) across the blood brain barrier, and had to be able to move
into Tris buffer for DAB reactions, and phosphate buffer gave consistently
poorer results.

Hope this helps.

Roger Moretz
Dept. of Toxicology
BI Pharmaceuticals, Inc.

I have no personal or financial interest in EM Sciences--just a satisfied
user.


On Tue, 15 Feb 2000 18:02:57 -0600 (CST), Heather A Owen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with
this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
}
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Wed Feb 16 16:21:34 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 16 Feb 2000 10:01:07 -0800
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Heather A Owen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Heather,

It's been a while but, back when I did such things, I had bouts of
non-uniform fixation when using biological grade glutaraldehyde solutions
even in plant tissues. However, the solutions had been kept much longer than
I'd now use and there are other preparation technique changes that confound
the determination of causality. In the cases where I've perfused small
animals, I've used EM grade GA since, as expensive as it is, it's cheaper
than repeating the experiment.

However, I did a full systemic perfusion. One random thought ( I haven't
tried it): What's the vascular volume of a rat brain? If you just need just
the brain, I'd think a little creative clamping (really small hemostats?) in
the thorax should let you reduce the volume needed to make even a large
experiment affordable with "the good stuff".

cheers and good luck,
John

John Heckman
MSM Department
Michigan State University





From daemon Wed Feb 16 16:21:52 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Wed, 16 Feb 2000 10:46:29 -0800 (PST)
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use Biological grade to perfuse rats (whole body, we haven't tried clamping
bits off, though that might be a good idea since we're interested in just the
head). After the perfusion is finished, we remove the interesting bit, which is
huge by EM standards, and fix some more in Karnowski's. Then we go on to osmium,
and continue processing. We get acceptable EM pictures at high magnification,
even though our blocks are so large and processing times are therefore
extended. So I can recommend Biological grade glutaraldehyde. Hope this helps.

Lesley Weston.



On Tue, 15 Feb 2000, Heather A Owen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
}
}
}
}




From daemon Wed Feb 16 20:58:18 2000



From: Larry Nittler :      nittler-at-lepvax.gsfc.nasa.gov
Date: Wed, 16 Feb 2000 17:09:21 -0500
Subject: TEM - Looking for ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello microscopy listserver-type people,

I am in the market for a used ion mill. Can anybody point me to one?

Thanks,
Larry

--
------------------------------------------------------------------------------
Larry R. Nittler Laboratory for Extraterrestrial Physics,
Code 691
Interstellar Dust Buster NASA Goddard Spaceflight Center
Greenbelt MD 20771
phone: 301-286-4572 fax:301-286-0212
nittler-at-lepvax.gsfc.nasa.gov http://www.ciw.edu/lrn/
------------------------------------------------------------------------------



From daemon Wed Feb 16 20:58:19 2000



From: RLevenson-at-cri-inc.com
Date: Wed, 16 Feb 2000 16:24:20 -0600
Subject: RE: autofluorescence quenching: spectral imaging can help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: RLevenson-at-cri-inc.com Subject: RE: autofluorescence quenching:
spectral imaging can help

You asked about strategies for reducing autofluorescence.

In addition to various strategies which can include using near-IR dyes like
Cy5.5 and exciting far in the red, you can also treat tissues with sodium
borohydride or toluidine blue (these latter observations I pass on from
others--have not tried them myself).

I have recently had very promising results using spectral imaging to
discriminate autofluorescence from desired fluorescence. [Caution: I am
describing a product sold by my company]. CRI makes a liquid crystal
tunable filter (LCTF) that can be used to collect a stack of images at
different wavelengths. This yields a spectrum at every pixel of an image.
(A product with similar capabilities is sold by Applied Spectral Imaging,
but uses a different technology.) CRI's new LCTF, the FluoroSpec, uses
special optics to combine polarization states; along with other
improvements, it has more than twice the light throughput compared to
previous models.

The sample was formalin-fixed brain probed with a Cy2-labeled antibody to a
low-abundance protein. By eye it was very difficult to distinguish the Cy2
signal from the bright green to green-orange autofluorescence. Using a
spectral data set (15 images from 510 nm to 650 nm at 10 nm intervals) and
linear combination algorithms for pixel-unmixing, it was possible to
completely separate the Cy2 and autofluorescence images.

I would be glad to share these images with any interested parties.

Richard


Richard Levenson, MD
Technical Director, Biomedical Systems
Cambridge Research & Instrumentation (CRI), Inc.
80 Ashford St.
Boston, MA 02134

Ph: (617) 787-5700; Fax: (617) 787-4488
rlevenson-at-cri-inc.com www.cri-inc.com




From daemon Wed Feb 16 20:58:23 2000



From: chuck burilla :      burilact-at-ix.netcom.com
Date: Wed, 16 Feb 2000 18:29:41 -0500
Subject: Re: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With respect to the Epson Stylus Pro 5000:

I just purchased one for printing images, x-ray maps, etc. obtained from my
new JEOL 8900 microprobe. Previous to this I was utilizing an Epson Photo
700 printer with my older Cameca MBX probe. I was very pleased with the 700
and for a few hundred dollars sure was worth it! Not a fast printer but gave
high quality photo-type prints.
After reading the 5000 specs I decided to get one for the new probe.
In a "nutshell" here are my observations:

I purchased just the 5000 printer (not the Fiery RIP server that interfaces
to it for network printing). Cost: around $3000. for the 5000 only.
The 5000 is a much more robust printer than the 700 type Epson models. It
can print up to 13"X19" formats. It is faster than the 700 series and the
ink cartridges and paper capacity are much greater.

To realistically compare the printers I ran a speed and print quality
comparison between my two models. I printed a full 8 1/2" X 11" full color
bleed consisting of 3 x-ray maps, a BSE image and an EDS spectrum, along
with some text. Printing on the same PC, under the same conditions the print
quality is about the same. (A little disappointing here). However the print
speed was a full 4 times faster on the 5000 as compared to the 700. Actual
print time with the 5000 was about 2.5 min. at 1440 DPI.
I found an excellent web site which compares various Epson Photo printers,
including the 5000 and 700 series, and demonstrates print quality issues
quite vividly:

http://www.tssphoto.com/sp/dg/news/dot_comp.html

All in all I believe the 5000 is a much higher caliber printer than the 700
series, but unfortunately the print quality is about the same.

One final note: the paper makes ALL the difference in the world with this
class of printers.
Email me if you need info and suggestions on paper issues...



Chuck Burilla
United Technologies Research Center
400 Silver Lane
East Hartford, CT 06108

(860) 610-7388
burilact-at-utrc.utc.com



-----Original Message-----
} From: Malcolm Haswell [mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Tuesday, February 15, 2000 9:03 AM
To: Microscopy (MSA)


I saw the Epson web spec and it looks quite impressive - especially as
they seem to be quoting speeds for real picture printing, not 5%
coverage or text.

My only concern is that Epson now market two printers with "10 years
light fastness" (the Epson Stylus photo 870 - A4 and 1270 A3) so you
have to wonder which is better for e.m. prints.

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

---------------------------------------------------------

Tom Reese wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
} but they claim it is much better than the much cheaper models many of
} us know and love. Would anyone know whether this is true of just
} hype-hard to tell from the published specs...? Thanks...Tom Reese



From daemon Wed Feb 16 20:58:31 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 16 Feb 00 15:54:53 -0800
Subject: General:Wall Posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

Does anyone know the name of a supplier of wall posters suitable for an EM lab. I am thinking of cut-out diagrams of TEM and SEM, history of EM (I think JEOL once had one like that), specimen preparation etc.
Regards,

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Feb 16 20:58:35 2000



From: oshel-at-terracom.net (Philip Oshel)
Date: Wed, 16 Feb 2000 19:57:42 -0600
Subject: Gordon Cougar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id UAA14463
for dist-Microscopy; Wed, 16 Feb 2000 20:00:35 -0600 (CST)
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


My apologies to the list, but: could Gordon Cougar email me? I get a fatal
error when trying to email you:
The following addresses had permanent fatal errors -----
} {gcouger-at-rfdata.net}
[...]
} 550 {gcouger-at-rfdata.net} ... User unknown

Thanks!

Phil

*** Be famous! Send a Tech Tip or article to Microscopy Today! ***
Philip Oshel
Technical Editor, Microscopy Today
P.O. Box 620068
Middleton, WI 53562-0068
Voice: days (608) 263-4162, evening (608) 833-2885
Fax (608) 836-1969 (please make sure my name is on any fax)

Address for UPS, FedEx, or other couriers:
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581

oshel-at-terracom.net
or
peoshel-at-facstaff.wisc.edu




From daemon Thu Feb 17 16:22:03 2000



From: Steve D'Angelo :      steve-at-equiprx.net
Date: Thu, 17 Feb 2000 03:17:28 -0800
Subject: General:Wall Posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,
My favorite has always been the DIATOME calendars.
Next are the Gatan calanders.
Dennis Kunkel has great original SEMs of diatomes etc, but I don't know if
he has any posters.
David Sharpe has the old standby insect SEMs.
The students at SanJoaquin Delta College have put together some good
calendars also.

} Date: 16 Feb 00 15:54:53 -0800
} From: Paul Webster {pwebster-at-mailhouse.hei.org}
} Subject: General:Wall Posters
} To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} X-Priority: 3
} MIME-Version: 1.0
} Reply-To: Paul Webster {pwebster-at-mailhouse.hei.org}
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ultra5.microscopy.com id RAA14282
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Very best regards,
Steve D'Angelo
Equipment Resurrection
650-738-0351

NOW at http://equiprx.net/




From daemon Thu Feb 17 01:30:04 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 17 Feb 2000 16:14:42 GMT+1200
Subject: DP fluid for JEOL 840

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello, Pumpers

Does anyone out there have direct experience of any benefit(s) gained
by the use of Santovac 5 in the diffusion pumps of a JEOL 840,
instead of the recommended Lion S?

And did they have to fiddle with either the heating elements or
heater supply voltages in order to acheive the benefits?

I know that Santovac is well thought of, but it's expensive, and my
experience of Lion S in an older JEOL (JXA-5A) has been very
positive. I think there's something in there about optimising the
pump design to a particular fluid.

As an economical alternative, mentioned in Wil Bigelow's book, has
anyone tried NEOVAC SY, marketed by Varian, in an 840?

Does anyone know what chemical family Lion S belongs to?

Perhaps someone from JEOL, or even from the Lion company might care
to reply.

cheers

Ritchie

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Feb 17 20:21:56 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 17 Feb 2000 17:04:35 -0800 (PST)
Subject: Mikros Manual available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I'm cleaning out some things in the laboratory and we have a Mikros Vacuum
Evaporator Manual C-20, 1964 here. We no longer have this evaporator, so
we no longer need the manual. If anyone would have need of it, please
email me personally and we'll send it out to you.

I'm amazed at the quality of this older manual - actual blue prints
included!

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Feb 18 20:56:58 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Sat, 19 Feb 2000 09:58:59 +1100
Subject: Re: DP fluid for JEOL 840

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ritchie,

There was a fairly extensive discussion about Dp oils on the listserver a
while ago. That should be in the archives somewhere.

}
} Does anyone out there have direct experience of any benefit(s) gained
} by the use of Santovac 5 in the diffusion pumps of a JEOL 840,
} instead of the recommended Lion S?
}

"Yes". We have been using Santovac 5 in our early 6400 machine for about 5
months now. The diffusion pumps appear to be the same as on an 840 and the
6400 had Lion S in it beforehand.

} And did they have to fiddle with either the heating elements or
} heater supply voltages in order to acheive the benefits?
}

No. The vacuum seems to be as good as ever without doing any of this. It
all looks very clean.

} I know that Santovac is well thought of, but it's expensive, and my
} experience of Lion S in an older JEOL (JXA-5A) has been very
} positive. I think there's something in there about optimising the
} pump design to a particular fluid.
}
} As an economical alternative, mentioned in Wil Bigelow's book, has
} anyone tried NEOVAC SY, marketed by Varian, in an 840?
}
} Does anyone know what chemical family Lion S belongs to?

That should all be in the archives somewhere, or perhaps on certain web
sites. Jim Darley and/or Will Bigelow had the answers.

}
} Perhaps someone from JEOL, or even from the Lion company might care
} to reply.
}

We were told that the Santovac 5 is more tolerant of the occasional gulp of
air.....so presumably it should last longer.

Cheers


Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Fri Feb 18 20:56:57 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Fri, 18 Feb 2000 17:59:22 -0600
Subject: Photoshop 5.5 Thumbnail Preview in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For imaging gurus:

I am using Photoshop 5.5 on a G3 Macintosh and can no longer get a preview
of the image when the file is selected in FILE } OPEN. What gives?

In previous versions (say 4.0 and earlier) when the file was selected, I
got a thumbnail preview of the image prior to opening it. This is very
convenient and I miss this capability.

Thanks,

John

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################




From daemon Sat Feb 19 13:13:31 2000



From: steve :      halifa-at-globalnet.co.uk
Date: Sat, 19 Feb 2000 12:32:11 -0600
Subject: Re Santovac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Santovac is a great oil to use in Jeol instruments. Backstreaming is
less and it will withstand a lot more abuse than Lion oil if the vac
system has a glitch. Just put a little less charge in than the normal Lion
oil. Regards Steve Parkins




From daemon Sat Feb 19 13:13:30 2000



From: Wright, Cheryl W :      CWright-at-Sikorsky.com
Date: Sat, 19 Feb 2000 12:32:33 -0600
Subject: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,

WOW, thanks soooo much for all the responses to my question. I have alot of
techniques to try so that should keep me busy for awhile. This is a very
informative site and I appreciate the help!!

Cheryl




From daemon Sun Feb 20 18:03:29 2000



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Sun, 20 Feb 2000 14:48:35 -0600
Subject: TEM Philips 300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Biological Sciences at the University of Iowa must find a
home for a Philips 300 Transmission Electron Microscope by June 2000. It
is in excellent condition and has been under service contract for nearly 30
years. We are remodeling our building and will no longer maintain support
for this instrument. Free to anyone willing to come and take it away (plus
Haskris chiller, carbon evaporator, film dessicator, etc.).

Please contact:
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242 USA
319-335-1070 (fax 319-335-1069)
dean-abel-at-uiowa.edu



From daemon Sun Feb 20 18:03:30 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Mon, 21 Feb 2000 09:49:50 +1100
Subject: Photoshop 5.5 Thumbnail Preview in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

Open the image in Photoshop and select SAVE AS. There are boxes to be
ticked, one of which is Mac thumbnail. My guess is that your problem
is here.

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Mon Feb 21 15:05:39 2000



From: Robert Champaign :      r-champaign-at-raytheon.com
Date: Mon, 21 Feb 2000 08:05:00 -0800
Subject: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are currently using Cool Prep in our Coolwell cooler system. This is a
liquid cleaner & slime preventer that helps keep mineral deposits to a
minimum in the cooling system. It also helps prevent corrosion in the
water passages without harming hoses, gaskets or seals. Since Coolwell is
no longer in business does anyone know of a replacement product for Cool
Prep.

Can we simply switch to ethylene glycol?
Robert Champaign
Raytheon
Electronic Systems Labs - Texas Region
r-champaign-at-raytheon.com
2501 W.University, MS 8011
McKinney Texas, 75070
972-952-3165



From daemon Mon Feb 21 15:05:41 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Mon, 21 Feb 2000 09:58:54 -0600
Subject: SEM-JEOL 845 TMP problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Y'all:
We have an JEOL 845 SEM (TMP pumped) and we have been having problems with the
TMP REV light staying on. Because of the microscopes' timing circuit, the
scope will shut off after 20 minutes. The shutting off/timing issue can be
overridden by keeping the key turned clockwise: the microscope will
eventually go to the correct vacuum, but the darn TMP light never goes out.
JEOL told us of a trick of depressing the standby button on the TMP
controller, sometimes remedies this situation, but it didn't work in our
case. We have been in contact with JEOL and so far we/they haven't figured
out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
oil, just to be sure). I have a few questions to ask of you 845 owners:
1) Have you seen this type of problem, and if so, how did you remedy it?
2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
have anything.

Your help will be greatly appreciated.
Mike Coviello
Lab Manager
UT Arlington
Arlington, TX
817 272-5496



From daemon Mon Feb 21 15:05:47 2000



From: Ziel, R. (Rainer) :      Rainer.Ziel-at-akzonobel.com
Date: Mon, 21 Feb 2000 19:55:17 +0100
Subject: LM: Video-Adapter for Zeiss Photo Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an old Photomicroscope from Zeiss. It's grey in color and about 20
years old, but works pretty good. Until now we have an old 1 inch video
camera on top. We want to change this video camera to a state of art
CCD-camera with 1/2 inch CCD-chip. To enlarge the field of view, we want to
use a video adapter with a magnification 0.5x. Unfortunately several
oprtical corrections are made within this adapter. Such an adapter is not
offered by Zeiss. Does anybody out there knows a company that sells such a
special adapter or can build one?

Kind Regards

Rainer

--------

Rainer Ziel
Acordis Analytical Services Obernburg
63784 Obernburg
Germany
Phone: 06022/812645
email: rainer.ziel-at-acordis.com





From daemon Mon Feb 21 15:05:47 2000



From: Ziel, R. (Rainer) :      Rainer.Ziel-at-akzonobel.com
Date: Mon, 21 Feb 2000 19:55:17 +0100
Subject: LM: Video-Adapter for Zeiss Photo Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an old Photomicroscope from Zeiss. It's grey in color and about 20
years old, but works pretty good. Until now we have an old 1 inch video
camera on top. We want to change this video camera to a state of art
CCD-camera with 1/2 inch CCD-chip. To enlarge the field of view, we want to
use a video adapter with a magnification 0.5x. Unfortunately several
oprtical corrections are made within this adapter. Such an adapter is not
offered by Zeiss. Does anybody out there knows a company that sells such a
special adapter or can build one?

Kind Regards

Rainer

--------

Rainer Ziel
Acordis Analytical Services Obernburg
63784 Obernburg
Germany
Phone: 06022/812645
email: rainer.ziel-at-acordis.com





From daemon Mon Feb 21 15:47:03 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 15:10:21 -0600
Subject: Administrivia: Server is acting strange...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

Yes I know the server appears to be acting up (i.e. not sending
any mail out).. I'm looking into the problem.

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Feb 21 17:08:33 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Mon, 21 Feb 2000 16:59:58 -0600
Subject: LM: COURSES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FORWARDED TO LISTSERVER,
PLEASE RESPOND TO THE ADDRESS SHOWN BELOW:

{randy_anderson-at-ameritech.net}

I am a member of MSA and I work for the University of Chicago in the
section of Nephrology. I was wondering if you would know of any
universities or other types of institutions that would offer summer
courses for the beginner and advance areas of immunofluorescence
microscopy. Any help that you can give me in matter would greatly be
appreciated.

Thank you

Randy Anderson

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################




From daemon Mon Feb 21 17:08:34 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 17:07:02 -0600
Subject: Testing using TestList

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a test to 3 zaluzec addresses and the archive.
5:06 pm




From daemon Mon Feb 21 17:25:15 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 21 Feb 2000 15:17:47 -0800
Subject: Re: SEM-JEOL 845 TMP problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a copy can send you.

The JEOL Schematics are somewhat ambiguous as the logic from the turbo pump
activates several relays. The relays are NOT connected in the manner that one
would think, i.e. when the turbo pump speed is 80% the relay is activated and
signals the vacuum logic that all is well.

Earl Weltmer

Michael Coviello wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Y'all:
} We have an JEOL 845 SEM (TMP pumped) and we have been having problems with the
} TMP REV light staying on. Because of the microscopes' timing circuit, the
} scope will shut off after 20 minutes. The shutting off/timing issue can be
} overridden by keeping the key turned clockwise: the microscope will
} eventually go to the correct vacuum, but the darn TMP light never goes out.
} JEOL told us of a trick of depressing the standby button on the TMP
} controller, sometimes remedies this situation, but it didn't work in our
} case. We have been in contact with JEOL and so far we/they haven't figured
} out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
} relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
} oil, just to be sure). I have a few questions to ask of you 845 owners:
} 1) Have you seen this type of problem, and if so, how did you remedy it?
} 2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
} the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
} have anything.
}
} Your help will be greatly appreciated.
} Mike Coviello
} Lab Manager
} UT Arlington
} Arlington, TX
} 817 272-5496



From daemon Mon Feb 21 18:20:00 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Mon, 21 Feb 2000 17:33:42 -0800
Subject: Position available: EM-TECHNICIAN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ELECTRON MICROSCOPY TECHNICIAN
A full time position for a senior research technician is available at
the University of Chicago in the Department of Molecular Genetics and
Cell Biology. The lab of Elaine Fuchs Ph.D. is interested in the
cytoskeleton, in cell-cell adhesion and in the cell biology of skin
development.
The successful candidate will perform routine transmission electron
microcopy, including specimen acquisition, tissue processing,
ultramicrotomy, operation of a Philips CM120 electron microscope and
darkroom procedures/digital imaging. In addition he/she will be trained
in on-section immunolabeling using Lowicryl K4M.
Applicant Qualifications: Minimum requirements are a Bachelors degree
and 2 years of relevant experience.
If you are motivated to work in an excellent scientific environment with
state of the art instrumentation please contact:

Christoph Bauer Ph.D.
University of Chicago,
Department of Molecular Genetics and Cell Biology
5841 S. Maryland Ave/MC 1028
Chicago, Il 60637

Phone: 773 834 2896
Fax: 773 702 0141
Email:cbauer-at-midway.uchicago.edu



From daemon Mon Feb 21 18:31:28 2000



From: Robert Dourmashkin :      robert-at-bobsydnee.demon.co.uk
Date: Mon, 21 Feb 2000 18:18:47 -0600
Subject: MT2 Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anybody need a MT2 Porter-Blum ultramicrotome, in running order but
no binocular? Yours for the asking and transport.

Robert Dourmashkin,
Section of Academic Virology,
Royal London School of Medicine,
40 Turner Street,
Whitechapel London E1 2AD.
--
Robert Dourmashkin




From daemon Mon Feb 21 18:31:28 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:16:54 -0600
Subject: Administrivia: Restarting the Server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

I'm reinitializing the server. You may see some old and/or
duplicate messages. I will try to restore those that I think
did not get through. If you tried to post a message in the last
day or so and haven't seen it please wait ~ 12 hours after
that time if you do not see it appearby then , you should
presume it was lost and repost your original
question/comment.

Sorry...

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Feb 21 18:31:29 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 21 Feb 2000 18:18:12 -0600
Subject: Fwd: SEM/TEM Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} } Ms. Lalini Pillayi Would like to take both a TEM and SEM short
} } course. She is located at the at the US ARmy Dental Research Great
} } Lakes Lab.
} } Her applications will be both biological and biological
} } combined with material. If you offer such a course please contact her
} } directly at
} } lalini70-at-hotmail.com
} }
} } Thanks

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611






From daemon Mon Feb 21 18:31:29 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 21 Feb 2000 18:17:32 -0600
Subject: Re: SEM-JEOL 845 TMP problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I have a copy can send you.

The JEOL Schematics are somewhat ambiguous as the logic from the turbo pump
activates several relays. The relays are NOT connected in the manner that one
would think, i.e. when the turbo pump speed is 80% the relay is activated and
signals the vacuum logic that all is well.

Earl Weltmer

Michael Coviello wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Y'all:
} We have an JEOL 845 SEM (TMP pumped) and we have been having problems
} with the
} TMP REV light staying on. Because of the microscopes' timing circuit, the
} scope will shut off after 20 minutes. The shutting off/timing issue can be
} overridden by keeping the key turned clockwise: the microscope will
} eventually go to the correct vacuum, but the darn TMP light never goes out.
} JEOL told us of a trick of depressing the standby button on the TMP
} controller, sometimes remedies this situation, but it didn't work in our
} case. We have been in contact with JEOL and so far we/they haven't figured
} out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
} relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
} oil, just to be sure). I have a few questions to ask of you 845 owners:
} 1) Have you seen this type of problem, and if so, how did you remedy it?
} 2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
} the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
} have anything.
}
} Your help will be greatly appreciated.
} Mike Coviello
} Lab Manager
} UT Arlington
} Arlington, TX
} 817 272-5496




From daemon Mon Feb 21 18:31:29 2000



From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Mon, 21 Feb 2000 18:18:24 -0600
Subject: Kevex Preamp Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Good morning:

We would like to resurrect 2 older Kevex SiLi detectors for scintillation
counting, and have lost or misplaced the manuals for the preamp output
connectors, ie. voltages and pinouts.
The model numbers of the preamps are 2002 and 2003.

If anyone has this info, could you please fax or email?

Thanks in advance

Fred Pearson

********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1


email: eoptics-at-mcmaster.ca
phone: (905) 525-9140 ext. 24609
fax: (905) 521-2773
********************************************************




From daemon Mon Feb 21 18:31:30 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Mon, 21 Feb 2000 18:21:28 -0600
Subject: Position available: EM-TECHNICIAN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



ELECTRON MICROSCOPY TECHNICIAN
A full time position for a senior research technician is available at
the University of Chicago in the Department of Molecular Genetics and
Cell Biology. The lab of Elaine Fuchs Ph.D. is interested in the
cytoskeleton, in cell-cell adhesion and in the cell biology of skin
development.
The successful candidate will perform routine transmission electron
microcopy, including specimen acquisition, tissue processing,
ultramicrotomy, operation of a Philips CM120 electron microscope and
darkroom procedures/digital imaging. In addition he/she will be trained
in on-section immunolabeling using Lowicryl K4M.
Applicant Qualifications: Minimum requirements are a Bachelors degree
and 2 years of relevant experience.
If you are motivated to work in an excellent scientific environment with
state of the art instrumentation please contact:

Christoph Bauer Ph.D.
University of Chicago,
Department of Molecular Genetics and Cell Biology
5841 S. Maryland Ave/MC 1028
Chicago, Il 60637

Phone: 773 834 2896
Fax: 773 702 0141
Email:cbauer-at-midway.uchicago.edu




From daemon Mon Feb 21 18:31:30 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:22:34 -0600
Subject: JEOL 845 SEM (TMP Light)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Y'all:
We have an JEOL 845 SEM (TMP pumped) and we have been having problems with the
TMP REV light staying on. Because of the microscopes' timing circuit, the
scope will shut off after 20 minutes. The shutting off/timing issue can be
overridden by keeping the key turned clockwise: the microscope will
eventually go to the correct vacuum, but the darn TMP light never goes out.
JEOL told us of a trick of depressing the standby button on the TMP
controller, sometimes remedies this situation, but it didn't work in our
case. We have been in contact with JEOL and so far we/they haven't figured
out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
oil, just to be sure). I have a few questions to ask of you 845 owners:
1) Have you seen this type of problem, and if so, how did you remedy it?
2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
have anything.

Your help will be greatly appreciated.
Mike Coviello
Lab Manager
UT Arlington
Arlington, TX
817 272-5496




From daemon Mon Feb 21 18:35:34 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:23:47 -0600
Subject: Prep of rat brains for TEM using vascular perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Heather A Owen wrote:
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Heather,

It's been a while but, back when I did such things, I had bouts of
non-uniform fixation when using biological grade glutaraldehyde solutions
even in plant tissues. However, the solutions had been kept much longer than
I'd now use and there are other preparation technique changes that confound
the determination of causality. In the cases where I've perfused small
animals, I've used EM grade GA since, as expensive as it is, it's cheaper
than repeating the experiment.

However, I did a full systemic perfusion. One random thought ( I haven't
tried it): What's the vascular volume of a rat brain? If you just need just
the brain, I'd think a little creative clamping (really small hemostats?) in
the thorax should let you reduce the volume needed to make even a large
experiment affordable with "the good stuff".

cheers and good luck,
John

John Heckman
MSM Department
Michigan State University





From daemon Mon Feb 21 18:35:42 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:24:19 -0600
Subject: OIL for use in JEOL's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Santovac is a great oil to use in Jeol instruments. Backstreaming is
less and it will withstand a lot more abuse than Lion oil if the vac
system has a glitch. Just put a little less charge in than the normal Lion
oil. Regards Steve Parkins






From daemon Mon Feb 21 18:35:48 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:25:09 -0600
Subject: More on Rat Brain Prep..

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We use Biological grade to perfuse rats (whole body, we haven't tried clamping
bits off, though that might be a good idea since we're interested in just the
head). After the perfusion is finished, we remove the interesting bit, which is
huge by EM standards, and fix some more in Karnowski's. Then we go on to
osmium,
and continue processing. We get acceptable EM pictures at high magnification,
even though our blocks are so large and processing times are therefore
extended. So I can recommend Biological grade glutaraldehyde. Hope this helps.

Lesley Weston.





From daemon Mon Feb 21 18:35:55 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:25:57 -0600
Subject: CCD Camera on Photomicroscope from Zeiss

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have an old Photomicroscope from Zeiss. It's grey in color and about 20
years old, but works pretty good. Until now we have an old 1 inch video
camera on top. We want to change this video camera to a state of art
CCD-camera with 1/2 inch CCD-chip. To enlarge the field of view, we want to
use a video adapter with a magnification 0.5x. Unfortunately several
oprtical corrections are made within this adapter. Such an adapter is not
offered by Zeiss. Does anybody out there knows a company that sells such a
special adapter or can build one?

Kind Regards

Rainer

--------

Rainer Ziel
Acordis Analytical Services Obernburg
63784 Obernburg
Germany
Phone: 06022/812645
email: rainer.ziel-at-acordis.com





From daemon Mon Feb 21 18:36:19 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:26:40 -0600
Subject: geometry settings for a Kevex Quantum detector for JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone have the geometry settings for a Kevex Quantum detector on a
JEOL 2000FX? I am using DTSA and need the values. I would like to have the
sample to detector distance and the azimuthal angle. I am using +45 for the
azimuthal angle and 90 for the detector angle. Are these correct? I know
that the takeoff angle is 70.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Mon Feb 21 18:36:40 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:27:29 -0600
Subject: looking for used ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello microscopy listserver-type people,

I am in the market for a used ion mill. Can anybody point me to one?

Thanks,
Larry

--
------------------------------------------------------------------------------
Larry R. Nittler Laboratory for Extraterrestrial Physics,
Code 691
Interstellar Dust Buster NASA Goddard Spaceflight Center
Greenbelt MD 20771
phone: 301-286-4572 fax:301-286-0212
nittler-at-lepvax.gsfc.nasa.gov http://www.ciw.edu/lrn/
------------------------------------------------------------------------------




From daemon Mon Feb 21 18:46:00 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:28:06 -0600
Subject: RE: autofluorescence quenching:spectral imaging can help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: RLevenson-at-cri-inc.com Subject: RE: autofluorescence quenching:
spectral imaging can help

You asked about strategies for reducing autofluorescence.

In addition to various strategies which can include using near-IR dyes like
Cy5.5 and exciting far in the red, you can also treat tissues with sodium
borohydride or toluidine blue (these latter observations I pass on from
others--have not tried them myself).

I have recently had very promising results using spectral imaging to
discriminate autofluorescence from desired fluorescence. [Caution: I am
describing a product sold by my company]. CRI makes a liquid crystal
tunable filter (LCTF) that can be used to collect a stack of images at
different wavelengths. This yields a spectrum at every pixel of an image.
(A product with similar capabilities is sold by Applied Spectral Imaging,
but uses a different technology.) CRI's new LCTF, the FluoroSpec, uses
special optics to combine polarization states; along with other
improvements, it has more than twice the light throughput compared to
previous models.

The sample was formalin-fixed brain probed with a Cy2-labeled antibody to a
low-abundance protein. By eye it was very difficult to distinguish the Cy2
signal from the bright green to green-orange autofluorescence. Using a
spectral data set (15 images from 510 nm to 650 nm at 10 nm intervals) and
linear combination algorithms for pixel-unmixing, it was possible to
completely separate the Cy2 and autofluorescence images.

I would be glad to share these images with any interested parties.

Richard


Richard Levenson, MD
Technical Director, Biomedical Systems
Cambridge Research & Instrumentation (CRI), Inc.
80 Ashford St.
Boston, MA 02134

Ph: (617) 787-5700; Fax: (617) 787-4488
rlevenson-at-cri-inc.com www.cri-inc.com




From daemon Mon Feb 21 18:46:08 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:29:00 -0600
Subject: Help on fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi all. I have just joined this list and am posting without the usual
"lurking time" due to time constraints - please forgive if recently
covered. I am also a novice in all microscopy techniques, especially
fluorescence.

I am trying to detect GFP-containing cells in liver tissue and having
some problems.

1. I can't determine the spectra for the filter blocks I am using (on a
Nikon Eclipse TE300 with TE-FM Epifluorescence attachment). The blocks
are marked "UV", "B-2" (blue from source, green in field) and "G" (green
from source, red in field). I have e-mailed Nikon and to be fair
they've only had a few days but I'm under some pressure. No help from
the manual. I have been using B-2 for GFP.

2. I get quite a lot of background fluorescence even with frozen
sections (fixed in neutral buffered formalin). Can anyone suggest a way
to reduce this? (eg any extra filters?)

3. I have read conflicting opinions on fixation. Most previous work has
used thick sections (50 microns cut eg with Vibratome, ? to avoid having
to embed tissue blocks). GFP is interfered with by acetone (and probably
other organic solvents) but I would have thought that after fixation
with formalin it should be stabilized and resistant to the
xylene/alcohol used in paraffin embedding. I have seen fluorescence
retained after this treatment but perhaps it can be improved.

4. For those with liver fluoro experience - on "UV" setting I see bright
fluorescence which photobleaches. I believe I am looking at retinoids in
stellate cells, although the bleaching is incomplete and a bit slower
than I have seen before. Can anyone confirm this?

Apologies for long post. Any help much appreciated.

David Lockwood
University of Qld Dept of Surgery
PA Hospital Brisbane Australia





From daemon Mon Feb 21 19:02:28 2000



From: A. Greene :      ablue-at-io.com
Date: Monday, February 21, 2000 10:02 AM
Subject: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Robert,
Preparation of the water in water chillers is a strange world of its own and
you may get many responses to your question. The majority of my experience
has been with the Haskris systems but the chemistry may be the same. In
about 30 years of looking after many chillers, my formula for success goes
like this:
1 - Use deionized or distilled water. The water will leach enough products
from the hoses, microscope lenses, pumps and so forth to reach a equilibrium
and then be "ideally suited" for your system.
2 - Don't use any fungicide or other bug killer because it will slime up the
works.
3 - Put 4 or 5 drops of oil on top of the water reservoir. This will form a
barrier by creating a monolayer on top of the water and keep things from
growing in the water.
I have used this proceedure in tropical climates and in the north and in
both dry and humid environments and it has always worked very well. I can
almost gurantee you that ethyline glycol will gum up the works.

Best wishes and good luck.

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200 Phone:512-282-5507 FAX 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIRS


-----Original Message-----
} From: Robert Champaign {r-champaign-at-raytheon.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Mon Feb 21 23:39:42 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 23:38:16 -0600
Subject: looking for a bad address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This is a short test

11:38 pm




From daemon Mon Feb 21 23:48:26 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 00:21:26 -0600
Subject: another test but to microscopy@sparc5.microscopy.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


another test but to microscopy-at-sparc5.microscopy.com




From daemon Tue Feb 22 03:55:33 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Tue, 22 Feb 2000 10:52:37 +0100 (MET)
Subject: TEM EELS:How to focus ESI images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I'm using a CM30 FEG with GIF PEELS atached to it. I'm getting confused
about focussing an energy filtered image.

GATAN proposes to focus the image on the TV camere at a loss of
100eV.Apparently it will be focused more or less OK at a higher loss say
741eV.
DM uses the voltage offset which changes the HT of the microscope. So as
far as I understand the focus should be OK for every energy loss(no
refocussing should be needed at all) . Although in practice it is not. The
trick with focussing at 100eV works better than not focussing at all but
it is not satisfactory for high spatial resolution.

In the Phd of M.Schenner (TU Wien "The quantification problem in EELS
Microanalysis") I found a callibration procedure to find a defocus to
correct for chromatic abberation as a function of the energy loss you are
interested in. But he seems to use the drift tube in the GIF.

The question now: How should I focus my ESI images and what is the
explanation behind it?

Thanks in advance,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************



From daemon Tue Feb 22 05:55:15 2000



From: ALEX BLACK :      ALEXANDER.BLACK-at-NUIGALWAY.IE
Date: Tue, 22 Feb 2000 11:29:17 +0000 (GMT)
Subject: Quinacrine fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We are considering trying to label aspects of innervation using a
quinacrine fluorescence technique. So far the preliminary trials have not gone
well. Could anyone give advice on this, especially on a source for a reliable
fluorescent quinacrine dye?


Thanks in advance

Alex Black
Department of Anatomy
National University of Ireland, Galway



From daemon Tue Feb 22 05:45:16 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 22 Feb 2000 08:11:57 -0500
Subject: Re: LM: COURSES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,

We have specialists in this area who can offer customized, on-site courses,
using your equipment and speaking specifically to your application. If you
are interested in further details, or in how U. Chi can sponsor such a
course, please contact me directly.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 04:59 PM 2/21/00 -0600, John J. Bozzola wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Feb 22 07:25:03 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 22 Feb 2000 08:16:36 -0500
Subject: RE: autofluorescence quenching:spectral imaging can help

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From daemon Tue Feb 22 07:55:03 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Monday, February 21, 2000
Subject: Re: Prep of rat brains for TEM using vascular perfusion

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I perfused dozens of rat brains over the years. I would recomend using the purified glutaraldehyde. However, you can use a slightly less concentrated solution since the fixation is so much more efficient tham immersion fixation. We primarily fixed for ICC but only the choice of fix would vary not the method. For normal ultrastructure, I would try 2-3% Glut or half-strength Karnovsky's in a phosphate buffer system with added NaCl and Mg at physiological concentrations and see which fixation I prefered by examining the desired tissues prior to fixing critical animals.

We would anesthetize the animal, open the chest cavity and clamp off the descending aorta, thus limiting the perfusion to the upper half of the animal. The cannula would then be inserted into a small slit in the left ventricle, positioned up into the aorta, and held with a hemostat. Perfusion lines should have been filled with normal saline or ringers so that as soon as the cannula is in place, the flow can be started to wash out the blood. A small slit is made in the right atrium to permit release of venous blood, etc. As soon as the return is relatively pale, switch to your fixative and run the desired amount through. The condition of the liver is a reasonable criteria for the efficiency of the perfusion. It should be very pale yellow as the blood is removed and should stiffen up within a few minutes. In fact the entire upper part of the animal should become quite stiff as the tissues are fixed. Figure on a couple of hundred mls. of fix per animal.

A couple of suggestions are to use the initial ringer wash and fixatives at room temperature or slightly above so as not to cause vessels to contract. Do any further washes and post fix the portions of interest at 4oC. Also make sure you have a consistant but gental perfusion rate so as not to break delicate capillaries. Usually this can be accomplished just by raising the vessels containing the buffer and fix above the animal and let gravity do the rest.
Good luck,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------



Heather A Owen wrote:
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Heather,

It's been a while but, back when I did such things, I had bouts of
non-uniform fixation when using biological grade glutaraldehyde solutions
even in plant tissues. However, the solutions had been kept much longer than
I'd now use and there are other preparation technique changes that confound
the determination of causality. In the cases where I've perfused small
animals, I've used EM grade GA since, as expensive as it is, it's cheaper
than repeating the experiment.

However, I did a full systemic perfusion. One random thought ( I haven't
tried it): What's the vascular volume of a rat brain? If you just need just
the brain, I'd think a little creative clamping (really small hemostats?) in
the thorax should let you reduce the volume needed to make even a large
experiment affordable with "the good stuff".

cheers and good luck,
John

John Heckman
MSM Department
Michigan State University








From daemon Tue Feb 22 07:55:03 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 07:38:34 -0600
Subject: Administrivia: Still debugging

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Folks...

Still in a debug mode this is a very peculiar problem.
This test is going to the entire distribution..

Sorry

Nestor




From daemon Tue Feb 22 18:43:39 2000



From: John Catino :      jwcatino-at-concentric.net
Date: Tue, 22 Feb 2000 19:42:06 -0500
Subject: Enlarger and Print Processor Sale

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I have a Beseler 45M Enlarger and Kodak Royal Print Processor for sale.
Both are in working condition. We are going digital! Equipment is
located in Easton, PA.




John Catino




From daemon Tue Feb 22 18:43:39 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 18:42:08 -0600
Subject: another test of Microscopy

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This is a second test without the 15 minute delay/queus




From daemon Tue Feb 22 18:48:47 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 18:47:07 -0600
Subject: Testing at 6:47

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Testing at 6:47 to Microscopy




From daemon Tue Feb 22 19:38:42 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 19:20:37 -0600
Subject: Administrivia: DNS restored (I think)

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Colleagues....

Well the DNS (Domain Name Server) failed and was probably
pretty sick over most of last week. Alot of mail probably just
ended up in a black hole somewhere on the Net. Some of you
may have gotten messages, but I see that most of the mail
I sent out during my trip DownUnder to the ACEM-16 meeting
in Canberra never made it to it's destination.

The DNS "looks" fixed now but I'm not confident enough to proclaim
all as back to normal yet. Let's see if this message actually
gets through to everyone this time.

Sorry gang....

Nestor
Your Friendly (Frustrated) Neighborhood SysOp




From daemon Tue Feb 22 21:30:38 2000



From: Toby Knight :      tknight-at-waite.adelaide.edu.au
Date: Wed, 23 Feb 2000 13:32:59 +1030 (CST)
Subject: Sudan Black staining and fixing

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Greetings,

I have 2 queries pertaining to the use of the lipid colourant Sudan Black
B, I hope someone can be of some help:

1. I have been able to stain oil traces in the glands of citrus fruit BUT
the epoxy resin Procure/Araldite also stains quite strongly. Is this
typical of epoxy resins and would Spurrs show the same problem?

- Should I reduce the staining time from 30 mins?
- Adjust the temperature from 60 degrees C for staining?
- Rinse my sections for longer with 70% ethanol after staining?

2. I have used osmium tetroxide to fix lipids in the citrus rind tissues,
with mixed results.
I have also seen some success in oil fixation by applying Sudan Black B
to the tissue prior to chemical fixation. I assume it binds and reduces
the oil solubility. I am going to test this by treating tissue with SBB
prior and post chemical fix, and comparing.

- SBB is made up in 70% ethanol. As a postfix, would it be most logical
to introduce it at the 70% step in my ethanol dehydration series? (10 to
100%)

thanks kindly, Toby Knight (struggling PhD).


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
The University of Adelaide
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064
AUSTRALIA

Tel: +61 8 8303 6668 or 8303 6665
HVO: +61 8 8303 7242
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------




From daemon Wed Feb 23 07:45:46 2000



From: Dr. Manfred Rohde :      mro-at-GBF.de
Date: Wed, 23 Feb 2000 07:26:57 +0100
Subject: Immuno-labeling of glutaraldehyd, osmium embedded samples in

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Dear Microscopist,

has anyone out there any experiences with immuno-labeling of osmificated and
Spurr embedded samples. We have tried out etching, oxidizing sections with
hydrogen peroxide (10% for 15 min) or sodium methylat (1% for 15 min)
without any significant increase of label intensity (Ab used is polyclonal
from rabbit, protein A purified)

Any suggestions are welcome. Manfred



From daemon Wed Feb 23 07:45:48 2000



From: Fatima Merchant :      merchant-at-persci.com
Date: Wed, 23 Feb 2000 07:48:45 -0600
Subject: Re: pinhole grids

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Nestor,

It made it her ok. I thought I was getting pretty good
service all week. But I know what evils hide in name
servers. Not nearly to the extent you do:)

As always you efferots are welcome. You provide the
pipe for one of the best resorces on the net.

Gordon

G. C,. Couger
624 Cheyenne
Stillwater, OK 74075-1411
405 624 2855 between 3:00 pm to 1:00 am CST
405 742 2758 cell
----- Original Message -----
} From: "Nestor J. Zaluzec" {zaluzec-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 22, 2000 7:20 PM



}
} Dear All:
}
} I was wondering if anyone out there is aware of a company
} that sells pinhole grids. I am looking for grid(2D) of
} pinholes that are approximately 15-20 microns in diameter
} with a center to center separation of about 150-200 microns.
}
} I would greatly appreciate any information that you could
} provide regarding the commercial availability of similar
} pinhole grids.
}
} TIA,
} Fatima Merchant
}
}
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
}
} Fatima Merchant, Ph.D.
} Senior Research Engineer
} Perceptive Scientific Instruments, Inc.
} 2525 South Shore Blvd., Suite 100
} League City, Texas 77573
}
} Telephone: (281) 334-3027 Ext: 230
} Toll Free: (800) 288-3027 Ext: 230
} Facsimile: (281) 538-2222
} Email: merchant-at-persci.com
}
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
}
}




From daemon Wed Feb 23 08:26:47 2000



From: G Peranzi :      peranzi-at-bichat.inserm.fr
Date: Wed, 23 Feb 2000 08:25:18 -0600
Subject: KNEWS ABOUT GLOW DISCHARGE ACCESSORY

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-----Original Message-----
} From: Valdes Erica R Dr. SBCCOM
Sent: Tuesday, February 22, 2000 1:26 PM
To: 'microscopy-at-sparc5.Microscopy.Com'


We got a sputter-coater system (VG Microtech SC500) that we would like to
convert
into a glow discharge. Does anybody have done it already?
We got electronic schemes to do the High Voltage unit control, but we miss the
mecanical design to fit into our vacuum chamber (of the SC500). Does anybody
know
how to do?
We know that an acessory (for the Microtech) have been developped some years
ago
(GD350A) but it is not anymore available. What are the modification made to
use this
chamber?
Jean-Jacques LACAPERE
INSERM U 410
FacultŽ de MŽdecine X. Bichat
Paris FRANCE




From daemon Wed Feb 23 08:26:48 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 23 Feb 2000 08:25:41 -0600
Subject: TEM EELS:How to focus ESI images?

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Hello all,

I'm using a CM30 FEG with GIF PEELS atached to it. I'm getting confused
about focussing an energy filtered image.

GATAN proposes to focus the image on the TV camere at a loss of
100eV.Apparently it will be focused more or less OK at a higher loss say
741eV.
DM uses the voltage offset which changes the HT of the microscope. So as
far as I understand the focus should be OK for every energy loss(no
refocussing should be needed at all) . Although in practice it is not. The
trick with focussing at 100eV works better than not focussing at all but
it is not satisfactory for high spatial resolution.

In the Phd of M.Schenner (TU Wien "The quantification problem in EELS
Microanalysis") I found a callibration procedure to find a defocus to
correct for chromatic abberation as a function of the energy loss you are
interested in. But he seems to use the drift tube in the GIF.

The question now: How should I focus my ESI images and what is the
explanation behind it?

Thanks in advance,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************




From daemon Wed Feb 23 10:17:11 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 23 Feb 2000 09:37:47 -0500
Subject: Re: Immuno-labeling of glutaraldehyd, osmium embedded samples

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Material fixed like yours works only rarely. You might try etching with
saturated sodium metaperiodate for one hour followed by water washes before
incubating with the primary.
That is assuming that your antigen is not periodate sensitive.

At 07:26 AM 02/23/2000 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Wed Feb 23 10:17:13 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 23 Feb 2000 10:03:08 -0500
Subject: Re: pinhole grids

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Fatima Merchant wrote:

} } I was wondering if anyone out there is aware of a company
} } that sells pinhole grids. I am looking for grid(2D) of
} } pinholes that are approximately 15-20 microns in diameter
} } with a center to center separation of about 150-200 microns.
} }
} } I would greatly appreciate any information that you could
} } provide regarding the commercial availability of similar
} } pinhole grids.
} }

Dear Fatima,
I asked about the same question and got a reply from
Janko Otto (otto-at-quantifoil.com). He wrote me:

"I read a discussion about making holey films . We had
solving the problem using semiconductor lithographic techniques.

We have been producing QUANTIFOIL, a perforated foil with pre-definable
hole size shape and arrangement. The variation among the hole sizes is
small: the standard deviation for their distribution is well below 0.1
µm within a batch; the reproducibility for the average hole size between
batches is 0.2 µm. The shapes of the holes in the foils produced so far
are circular and square. Until now, their arrangements have been
orthogonal.

Foils with any desired hole size in the micrometer range, and with any
shape and arrangement can be fabricated. Any of these three parameter
can also be varied in a defined manner within one foil.

Until now, we produce three different types of QUANTIFOIL:

Type Hole Repetition distancee
um um
R1.2/1.3 ~1.2 2.5 (circular holes)
R2/2 ~2 4 (circular holes)
S7/2 ~7 9 (square holes)

QUANTIFOIL is generally delivered as a carbon foil; it can also be
obtained strengthened with plastic film.

If you interested in these perforated foil, I can send you some samples."

He may be able to produce what you want. I have not yet tried
his grids out, but I intend to when I get my specimens ready. I am not
affiliated with Quantifoil, just a potential user. Good luck.
Yours,
Bill Tivol





From daemon Wed Feb 23 10:17:14 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 23 Feb 2000 10:38:42 -0500 (EST)
Subject: Re: Cool Prep (Water Coolers)

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*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************

---------- Forwarded message ----------


} We are currently using Cool Prep in our Coolwell cooler system. This is a
} liquid cleaner & slime preventer that helps keep mineral deposits to a
} minimum in the cooling system. It also helps prevent corrosion in the
} water passages without harming hoses, gaskets or seals. Since Coolwell is
} no longer in business does anyone know of a replacement product for Cool
} Prep.
}
} Can we simply switch to ethylene glycol?

Dear Robert,
The suggestion of using distilled water and letting the system
come to equilibrium might work in a truly closed system composed of only
one material, but if you need to add water, or if you have several metals
in the system--as we do--this will be unsatisfactory in the long run.
We have found that Aqua-Treet 42 works very well in our Haskris system.
This product is made by Aqua Laboratories, Inc., PO Box 645, 8 Indus-
trial Way, Amesbury MA, (978) 388-3989. I have no relationship to this
company except as a satisfied user of their product.
Yours,
Bill Tivol



From daemon Wed Feb 23 18:10:14 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 23 Feb 2000 11:42:55 -0500
Subject: DTSA and EMMFF 1.1?

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I am running two copies of DTSA, one is an older version and one is the
newer one that will run on a power Mac. I am also fairly new to DTSA. The
old version does not have the MSA format capability for reading and writing
files, but the newer one does. However, the new one will read two versions
of MSA format, 1.0 and 1.1 but will only save in 1.1. Now I'm confused. I
asked Nestor where I could get the latest MSA description of the MSA format
and he sent me what I already have, EMMFF.total from of the EMMPDL/MAS-Lib
files. I know that Nestor was on that committee. So what gives with MSA
version 1.1 in the latest version of DTSA? Why don't they have the 1.0
option? The keywords are not even the same.



-Confused in Pittsburgh.


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Wed Feb 23 18:10:16 2000



From: wft03-at-health.state.ny.us
Date: Wed, 23 Feb 2000 12:13:50 -0500
Subject: Re: Cool Prep (Water Coolers)

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Why not use a liquid that has NO water in it. That solved my problem in
vibratory polishing of dissimilar metals in an alumina slurry. Ethylene
glycol or propylene glycol, (less toxic), are possibilities.


Dear Bernie,

That will work unless there is some microorganism which will

grow in ethylene glycol. One could also just buy commercial anti-freeze

and get some additional corrosion protection. I don't think that there

would be any problem with the circulation pump, since the viscosity

of glycol is similar to that of water. Keeping the fluid in the system for

some decades has different requirements from using it over a period

of hours, though.

Yours,

Bill Tivol




From daemon Wed Feb 23 18:10:16 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Wed, 23 Feb 2000 12:22:05 -0500
Subject: Re: DTSA and EMMFF 1.1?

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Look for a "Write MSA 1.0" plug-in in the "extras Folder" of your
DTSA download and move it to the "Plug-ins" folder at the same level
as DTSA.



At 11:42 AM -0500 2/23/00, Walck. Scott D. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Wed Feb 23 18:10:16 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 23 Feb 2000 11:39:28 -0600
Subject: (FE)SEM, sputter coating puzzle

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Greetings,
I wonder if anyone could explain the following observation
to me. I have been sputter coating with platinum and viewing the
sample with a FESEM (Hitatchi 4700, below lens type). In a certain
type of sample, the image appears darker than the background where
there is sample. Now these samples are perhaps a bit unusual. They
are very thin, starting off life as 1.75 um methacrylate sections of
a plant root adhered to a glass coverslip, followed by extraction in
hot acidic peroxide to remove all organic material except crystalline
cellulose. The cellulosic cell walls left behind on the coverslip are
nominally 100 nm thick and perhaps flattened further by the
processing. The cell walls are present in the sample as either cross
sections or as small regions laying in the plane of the coverslip.
These regions can be anywhere from the size of a cell (10 x 40 or so
microns) down to small portions thereof. All of this remaining cell
wall is darker than the surrounding background. I am putting on a
thin coat of Pt, somewhere around 2 nm, and imaging at 2 kV (I have
seen the same thing at 5 kV). It really puzzles me that my sample is
darker than background. Any explanations???

THanks,
Tobias
_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed Feb 23 18:10:18 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 23 Feb 2000 13:17:19 -0500
Subject: Food Science Prep.

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Hi,
Well I guess we never run out of new challenges when it comes to specimen prep. This one concerns a product that is made of a cereal bound together with a sugar syrup. The investigators want to see how the syrup behaves as to structural integrety over a period of time with and without the addition of various stabilizing components. Information would be collected at the light microscopic level.
We first thought of cryosectioning followed by examination using LM. However, the product is filled with air pockets and literally disintegrates when microtomed. It is easily cut into pieces at temperatures above freezing as long as the pieces are no thinner than3-4 mmAccempts to infiltrate it with OCT compoound prior to freezing were not satisfactory.
New thought is to use a low temperature resin to infiltrate the sample followed by UV or chemical polymerization. butThe resulting blocks could then be microtomed at room temperature and imaged. It would be desirable to cut sections of 4-8 µm in thickness which is too thick for an ultramicrotome with glass or diamond knives. Also UV polymerization would probably limit us to very small sample size which may be a problem.
Any suggestions would be appreciated as to resins to use which have low viscosity, could be used to infiltrate pieces of ~0.5-1cm sq, polymerize at low temperatures (+4 to 0oC range) using UV or chemical polymerization, and are preferably soft enough to section with a metal microtome blade. Any other suggestions of preparations techniques to try would be very appreciated.

Thanks in advance for your input.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Wed Feb 23 18:10:18 2000



From: Hank Adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 23 Feb 2000 11:14:42 -0600
Subject: TEMs

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Dear listservers, I need to determine the value of a Philips 410 that has
been on service contract until it was decommissioned last year. I need this
information for an insurance claim. Is there a company or dealer of used em
equipment that can help me?
Thanks
Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx



From daemon Wed Feb 23 18:10:19 2000



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Wed, 23 Feb 2000 14:29:55 -0500
Subject: GLOW DISCHARGE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry if this is a second post, but we just got an e-mail saying the
first was rejected as spam, so here goes:






Hi:

I want to be able to "Glow Discharge" carbon coated copper grids for the
purpose of making them hydrophilic and negatively charged, so that I can
spread aqueous suspensions on them. I have a Denton Vacuum Desk ll sputter
coater with a "etch" mode. The question I have for my fellow list servers
is: will "etching" the grids do the same thing as "Glow Discharging" them?
Many Thanks, Tim


Timothy Schneider, Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 Jeff Hall
1020 Locust St.
Philadelphia, Pa.
19107
215-503-4798
Timothy.Schneider-at-Mail.TJU.EDU



From daemon Wed Feb 23 18:10:21 2000



From: jillp-at-telecomedia.co.jp
Date: Wed, 23 Feb 2000 04:43:29 -0600
Subject: Version 5.0 is Now Available

Contents Retrieved from Microscopy Listserver Archives
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From daemon Wed Feb 23 18:10:21 2000



From: jillp-at-telecomedia.co.jp
Date: Wed, 23 Feb 2000 04:43:29 -0600
Subject: Version 5.0 is Now Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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To Unsubscribe your email address from our Opt-In listserver, see end of
message.

Dear Subscriber,

Introducing one the HOTTEST selling softwares of the year.

"NMS SOFTWARE" The Software no computer should be without!

Its Simple to Use and Easy to Understand.

Now you can quickly and easily have access to the most powerful internet investigation sites and databases available today!

"NMS v5.0" now with the latest IP Phone.

Make calls anywhere in the world for free.

Also with Unclaimed Money Locator, find out if
you are owed money or property in your state.

*******************************************************

The "N M S SOFTWARE" is absolutely astounding!

Every computer should have this!

Here's what you can obtain:
---------------------------
Files that the government has on you.

You know who "The F*I and.......

License plate number!

Get anyone's name and address with just a license plate number!

(Find that girl you met in traffic!)

Driving record!

Get anyone's driving record

Social security number!

Trace anyone by social security number!

Free Internet Access

Address!

Get anyone's address with just a name!

Unlisted phone numbers!

Get anyone's phone number with just a name - even unlisted numbers!

Locate!

Long lost friends, relatives, a past lover who broke your heart!

Send anonymous e-mail completely untraceable!

Investigate anyone!

Use the sources that private investigators use (all on the Internet)
secretly!

Ex-spouse!

Learn how to get information on an ex-spouse that will help you

win in court! (Dig up old skeletons)

Criminal search-background check!

Find out about your daughters boyfriend!

(or her husband)

Find out!

If you are being investigated!

Neighbors!

Learn all about your mysterious neighbors! Find out what they

have to hide!

People you work with!

Be astonished by what you'll learn about people you work with!

Education verification!

Did he really graduate college? Find out!
**********************************************************
Just insert the floppy disk and Go!
**********************************************************
Data Bases Cover the US and Canada.

You will be shocked and amazed by the secrets that can be

discovered about absolutely everyone! Find out the secrets

they don't want you to know! About others, about yourself!
--------------------------------------
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(ORDERS OUTSIDE THE USA, ADD $25.00)

DON'T WAIT TO GET STARTED... here's what to do:

STEP 1 - Compose a new message using the order form text below

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-----------------------------------------
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system by calling 708-562-1778
------------------------------------------
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} } } Mail-in Order Form { { {

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Address:

City:

State:

Zip:

E-mail address:

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[ ] Visa [ ] MasterCard [ ] Check

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From daemon Wed Feb 23 18:10:22 2000



From: Doug Cromey :      doug-cromey-at-ns.arizona.edu
Date: Wed, 23 Feb 2000 13:10:48 -0700
Subject: Can EELS, WDS or FT-IR localize As in Biol. tissues?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

We are interested in localizing arsenic in biological tissues (toxicology
research), but have been unable to come up with a technique that will give
us the cellular localization information we're looking for (sub-cellular
would be even better).

We have tried TEM with energy dispersive x-ray microanalysis and our
concentrations are apparently too low, so we are unable to detect arsenic.

A few years ago we looked into using the autometallographic techniques that
have been published by Dr. Gorm Dansher & his colleagues. We were told
that the photographic emulsions required were no longer available.

In a brainstorming session yesterday several techniques were bantered
about, but in truth we are not familiar enough with the techniques to know
if they would suit our needs. Does EELS have anything to offer, or perhaps
wavelength dispersive spectoscopy (WDS)? How about some of the new FT-IR
instruments? Are there any other techniques that might be able to localize
arsenic and/or indicate its state of oxidation?

Vendors are welcome to reply.

Yours,
Doug Cromey
....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Wed Feb 23 18:10:23 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Wed, 23 Feb 2000 15:28:52 -0500
Subject: Re: DTSA and EMMFF 1.1?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott & listers

Go to http://www.cstl.nist.gov/div837/837.02/dtsa.html and click on
"supporting files" page then download "preferences and plugins" you will
find 2 versions of the MSA write 1.0 (one is Kevex specific). It would
make life much easier on us microscopists if all manufacturers adopted the
MSA format.
Good luck,
Scott


} I am running two copies of DTSA, one is an older version and one is the
} newer one that will run on a power Mac. I am also fairly new to DTSA. The
} old version does not have the MSA format capability for reading and writing
} files, but the newer one does. However, the new one will read two versions
} of MSA format, 1.0 and 1.1 but will only save in 1.1. Now I'm confused. I
} asked Nestor where I could get the latest MSA description of the MSA format
} and he sent me what I already have, EMMFF.total from of the EMMPDL/MAS-Lib
} files. I know that Nestor was on that committee. So what gives with MSA
} version 1.1 in the latest version of DTSA? Why don't they have the 1.0
} option? The keywords are not even the same.
}
} -Confused in Pittsburgh.
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
} Walck-at-PPG.com
} (412) 820-8651 (office)
} (412) 820-8161 (fax)

For the weather watchers: Mostly sunny, 60 F, snow is melted away.

-------------------note: new mailing address----------------------
Scott Wight e-mail: scott.wight-at-nist.gov
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | fax: 301-417-1321
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Wed Feb 23 18:10:23 2000



From: Supanee Danviriyakul :      supaneed-at-foodsci.umass.edu
Date: Wed, 23 Feb 2000 15:40:10 -0500 (EST)
Subject: Image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I am doing the image analysis of SEM micrographs. The
micrographs contain both dense (light) and air cell (dark) areas. But two
areas are not discrete. I'm having a difficulty of quantifying those
separated fractions. I wonder if anyone has any ideas of how to
solve the problem.
Looking forward for your suggestions.
Regards,
Supanee



From daemon Wed Feb 23 18:10:25 2000



From: Rejane Magalh‹es Pimentel
Date: Wed, 23 Feb 2000 19:25:00 -0300
Subject: ellipse at Image Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
I need to know how to draw an ellipse to measure at Image Tool software.
Thanks a lot.
Rejane Pimentel
Rejane Magalh‹es Pimentel Galindo
Functional Plant Morphology
Universidade Federal Rural de Pernambuco
ggalindo-at-elogica.com.br
Rua Maria Carolina, 417/804
51020-220 Boa Viagem



From daemon Wed Feb 23 18:10:26 2000



From: Sandra Perkins :      skperkin-at-vt.edu
Date: Wed, 23 Feb 2000 17:06:32 -0500
Subject: epoxy embedding of rat eye

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thought I'd try this again in case it got lost in space:

Hi-

We are going to be processing rat eyes (from perfused animals) for
embedding in epoxy and I was wondering if anyone has any suggestions for
processing. We are interested in the retina, optic nerve, cornea and uveal
tract.

Thank you very much,
Sandy Perkins




From daemon Wed Feb 23 18:10:27 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 23 Feb 2000 16:03:17 -0600
Subject: Re: Image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ahh! That is always the rub, trying to get the areas of interest to stand
out as a distinct range of gray scales.

If it is just a question of noise pixels in what is otherwise a distinct
region, then you might try filtering (smoothing) your image before
thresholding. Or you might try changing your image acquisition protocol to
get less noisy images.

You might try a different signal to find something that is less subject to
overlaps. Usually I resort to backscattered electron imaging since the
dependence on atomic number of that signal typically results in distinct
gray levels.

If that won't do it, you might be able to perform some sort of image
processing to separate the regions. There are techniques available, but
there seems to be as much art as science to them. I would refer you to John
Russ' "Image Processing Handbook" or a similar text, because there is not a
short answer to that question.

Warren Straszheim

At 03:40 PM 2/23/2000 -0500, you wrote:
} Dear all,
} I am doing the image analysis of SEM micrographs. The
} micrographs contain both dense (light) and air cell (dark) areas. But two
} areas are not discrete. I'm having a difficulty of quantifying those
} separated fractions. I wonder if anyone has any ideas of how to
} solve the problem.
} Looking forward for your suggestions.
} Regards,
} Supanee



From daemon Wed Feb 23 19:00:26 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 23 Feb 2000 18:37:38 -0600
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: {"wft03-at-health.state.ny.us"-at-sparc5.microscopy.com}
To} Why not use a liquid that has NO water in it. That solved my problem
in
} vibratory polishing of dissimilar metals in an alumina slurry. Ethylene
} glycol or propylene glycol, (less toxic), are possibilities.
}
}
} Dear Bernie,
}
} That will work unless there is some microorganism which will
}
} grow in ethylene glycol. One could also just buy commercial anti-freeze
}
} and get some additional corrosion protection. I don't think that there
}
} would be any problem with the circulation pump, since the viscosity
}
} of glycol is similar to that of water. Keeping the fluid in the system
for
}
} some decades has different requirements from using it over a period
}
} of hours, though.

Glycols won't have near the heat capacity of water. I doubt
you will find a bug that will live in ethylene glycol but you can
probably find many that can live in propylene glycol.

Any cooling system needs to have the coolant changed
periodically. Following the manufactures recommendation
is the safe way to go.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Feb 23 19:00:27 2000



From: Ladd Resaerch :      ladres-at-worldnet.att.net
Date: Wed, 23 Feb 2000 18:17:38 -0600
Subject: Re: pinhole grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Fatima Merchant,

This is something that we can probably help you with. We do similar jobs
for several other companies.
If you could fax a drawing with the material, OD, Thickness, number of
holes, etc. to 1-802-878-8074 we can get you a quote.

Thanks,

John Arnott
Chairman
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc




From daemon Wed Feb 23 19:10:27 2000



From: Fang Qian :      fangq-at-tr.comm.mot.com
Date: Wed, 23 Feb 2000 18:57:07 -0600
Subject: Cryotransfer system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, There,

We are considering to purchase a cryotransfer system for our 2010F TEM.
There are two major suppliers: Gatan and Oxford. We are not sure which
one works better since this is the first time dealing with this
technique.

Could you please send me your comments and experiences on these two
vendors? Our samples are mostly soft tissues: Polymers, surfactants,
cell membranes, and proteins. Your help is highly appreciated.

Maoxu Qian
****************************
* Maoxu Qian, Ph.D. *
* Dept of MSE, box 352120 *
* University of Washington *
* Seattle, WA 98195 *
* mxq-at-u.washington.edu *
* (206)616-3973(phone) *
* (206)543-3100(fax) *
****************************



From daemon Wed Feb 23 19:50:19 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 23 Feb 2000 19:31:13 -0600
Subject: Tripod Polisher Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Workshop Objective

This course will cover all aspects of pre-thinning and focus on final
thinning via Tripod Polishing. Due to the limited class size and the
extensive hands-on opportunities, this course is well suited to novices as
well as advanced Tripodders. Attendees will also learn the latest
techniques available in ion milling, plasma cleaning and ion beam sputter
deposition and etching for TEM and SEM samples.

The workshop will be held in San Clemente, CA on May 26-27, 2000. Please
call for more information or visit our web site ( www.southbaytech.com )
for registration information.


***************************************************************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.




From daemon Wed Feb 23 20:10:23 2000



From: Maoxu Qian :      mxq-at-u.washington.edu
Date: Wed, 23 Feb 2000 20:04:56 -0600
Subject: Cryo holder TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} Hi, There,
}
} We are considering to purchase a cryotransfer system for our 2010F TEM.
} There are two major suppliers: Gatan and Oxford. We are not sure which one
} works better since this is the first time dealing with this technique.
} Could you please send me your comments and experiences on these two
} vendors? Our samples are mostly soft tissues: Polymers, surfactants, cell
} membranes, and proteins. Your help is highly appreciated.
}
} Maoxu Qian
} ****************************
} * Maoxu Qian, Ph.D. *
} * Dept of MSE, box 352120 *
} * University of Washington *
} * Seattle, WA 98195 *
} * mxq-at-u.washington.edu *
} * (206)616-3973(phone) *
} * (206)543-3100(fax) *
} ****************************
}
}
}
}
}




From daemon Thu Feb 24 08:25:15 2000



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Thu, 24 Feb 2000 11:41:30 +0530
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A related question from India. We have been using DM (demineralised) water in our JEOL 2000 EX II cooled by a Neslab chiller for the last ten years. This requires change every 6 months approximately. What additives can be used to prevent algae (? it is green and slimy and I am not a biologist) growth? Something obtainable locally (chemical name rather than trade names) will be most useful to me. Thanks very much.

-----Original Message-----
} From: William Tivol [SMTP:tivol-at-wadsworth.org]
Sent: Thursday, February 24, 2000 10:25 AM
To: Robert Champaign
Cc: microscopy-at-sparc5.microscopy.com


} We are currently using Cool Prep in our Coolwell cooler system. This is a
} liquid cleaner & slime preventer that helps keep mineral deposits to a
} minimum in the cooling system. It also helps prevent corrosion in the
} water passages without harming hoses, gaskets or seals. Since Coolwell is
} no longer in business does anyone know of a replacement product for Cool
} Prep.
}
} Can we simply switch to ethylene glycol?

Dear Robert,
The suggestion of using distilled water and letting the system
come to equilibrium might work in a truly closed system composed of only
one material, but if you need to add water, or if you have several metals
in the system--as we do--this will be unsatisfactory in the long run.
We have found that Aqua-Treet 42 works very well in our Haskris system.
This product is made by Aqua Laboratories, Inc., PO Box 645, 8 Indus-
trial Way, Amesbury MA, (978) 388-3989. I have no relationship to this
company except as a satisfied user of their product.
Yours,
Bill Tivol





From daemon Thu Feb 24 08:25:20 2000



From: Anita Garg :      Anita.Garg-at-lerc.nasa.gov
Date: Thu, 24 Feb 2000 07:55:24 -0500
Subject: Re: Allied Multiprep Polisher for TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues
Does anybody have experience on the Allied MultiPrep Polishing
System, especially in terms of getting a good TEM sample? How well do
the Allied's Precision Cross-Section Tool and TEM Wedge Polisher work
on this semi automatic multiprep polisher?
Also, how does their TEM Wedge Polisher compare with the South Bay
Tripod Polisher?
TIA
Anita
*******************************************
Dr. Anita Garg
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-3
21000 Brookpark Road
Cleveland, OH 44135
Phone : (216) 433-8908
Fax : (216) 977-7132
E-mail : Anita.Garg-at-grc.nasa.gov
*******************************************



From daemon Thu Feb 24 08:42:01 2000



From: Dan May :      dmay-at-iti-med.com
Date: Thu, 24 Feb 2000 08:26:39 -0600
Subject: PINHOLE GRIDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fatima Merchant,
I used to work for a company called Buckbee Mears. They have been making
tight tolerance electroformed Ni pinholes for many, many years. They are
located in St. Paul, MN and their phone number is 651-228-6400. Good luck.
Dan May




From daemon Thu Feb 24 08:42:02 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 24 Feb 2000 10:47:41 -0400
Subject: Re: epoxy embedding of rat eye

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hi-
}
} We are going to be processing rat eyes (from perfused animals) for
} embedding in epoxy and I was wondering if anyone has any suggestions for
} processing. We are interested in the retina, optic nerve, cornea and uveal
} tract.
}
} Thank you very much,
} Sandy Perkins

****************************
Hi Sandra,

Eyes are a real pain. There are so many layers of different cell types,
and each seems to infiltrate differently. Having worked on a fir number of
eyes, I woauld make the following suggestions:
Process the cornea separately. It is almost pure collagen. It will not
osmicate all that noticeably, so don't be surprised when it doesn't turn
dark brown or black. Give it long infiltration times, in vaccuum if
possible.

If you don't need the optic nerve attached to the eye, dissect it free and
process it. Give it slighlty longer than usual dehydration and
infiltration steps to be sure you'e got the myelin taken care of.

As for the rest: remove the lens and the vitreous so that you have good
acess to the retina. Be careful not to disrupt the retina when you remove
the vitreous. Once the lens is out, you can usually get the vitreous out
by gently blotting against a Kimwipe. You may want to split the orb into
hemispheres. Just be sure not to take any sections from the cut edge,
since you will disturb the structure there when you cut. Again, you'll
nees longer dehyration and infiltration times, since the sclera is really
tough. I use 30 minutes in each step of graded acetone, propylene oc=xide
then a graded infiltraion with PO:resin (Epon analog) 2:1 for 30 min, 1:1
overnight, 1:2 2 hours, pure resin overnight, on a rotator.

I'm sure there ore others out there, with their own "pet protocol" for
eyes. Its all a bit of magic and luck, isn't it?

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Thu Feb 24 08:42:03 2000



From: ferrari-at-comp.uark.edu (Michael Ferrari)
Date: Thu, 24 Feb 2000 08:30:12 -0800
Subject: WI objectives for BH-2 RFCA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Not sure this went out earlier...I've inherited an Olympus BH-2 RFCA
upright that I'd like to equip with WI lenses for live cell fluorescence
work. This is a 160mm tube length scope, and Olympus is now only
manufacturing the 60X WI fluor objective (I think/hope). Any leads to
sources of compatible WI objectives in the 10-60X range much appreciated.
Please reply offline. Thanks.

Mike

Michael Ferrari
Department of Biological Sciences
629 Science Engineering (SCEN)
University of Arkansas
Fayetteville, Arkansas 72701
Ph: 501-575-6372
Fax: 501-575-4010




From daemon Thu Feb 24 17:33:53 2000



From: amy-at-www.molbio.Vanderbilt.Edu (amy-at-helix.molbio.vanderbilt.edu)
Date: Thu, 24 Feb 2000 10:14:40 -0600
Subject: image processing - microdensitometer available

Contents Retrieved from Microscopy Listserver Archives
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We are interested in selling our Perkin-Elmer Model
1010M microdensitometer at a very reasonable price. It
was purchased as a reconditioned model from Perkin-Elmer
eight years ago and is in very good condition. Technical
manual is included.

If interested, please contact:

Amy Kendall
Department of Molecular Biology
Vanderbilt University
Box 1820, Station B
Nashville TN 37232
(615) 322-2012
amy-at-helix.molbio.vanderbilt.edu



From daemon Thu Feb 24 17:33:56 2000



From: Russell E. Cook :      recook-at-anl.gov
Date: Thu, 24 Feb 2000 11:03:00 -0600
Subject: RE: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


R. Divakar wrote, "A related question from India. We have been using DM
(demineralised) water in our JEOL 2000 EX II cooled by a Neslab chiller for
the last ten years. This requires change every 6 months approximately. What
additives can be used to prevent algae (? it is green and slimy and I am
not a biologist) growth? Something obtainable locally (chemical name rather
than trade names) will be most useful to me."

I quote here recommendations from the Haskris Co. (we have several Haskris
chillers):

1. If algae is present, flush the system with one of two alternatives: add
1 cup of chlorine bleach or one pint of hydrogen peroxide per 15 gallons of
water. Circulate 20-30 minutes. Drain the system and refill with clean
water.

2. To prevent regrowth of algae, one of the following additives are
recommended:
a. 10% solution of lab grade (no additives) ethylene glycol.
b. Dichlorophene. This is an insoluble white powder which is a
fungicide/bacteriacide. It should be sprinkled evenly across the entire
surface of the water in the chiller's tank.
c. 8 parts per million chlorine.

----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov




From daemon Thu Feb 24 17:33:56 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 24 Feb 2000 09:02:46 -0800
Subject: Re: (FE)SEM, sputter coating puzzle

Contents Retrieved from Microscopy Listserver Archives
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Dear Tobias,
The most logical explanation for this phenomenon is that the carbon-based
cell material is of a lower average atomic number, even with the thin Pt
coating, than the glass coverslip (SiO2) it is located on, also with the Pt
coat. The secondary electron yield is directly proportional to the average
atomic number, if topographic effects are not in evidence, which they won't
be on such a thin sample.
At 11:39 AM 2/23/00 -0600, you wrote:
} Greetings,
} I wonder if anyone could explain the following observation
} to me. I have been sputter coating with platinum and viewing the
} sample with a FESEM (Hitatchi 4700, below lens type). In a certain
} type of sample, the image appears darker than the background where
} there is sample. Now these samples are perhaps a bit unusual. They
} are very thin, starting off life as 1.75 um methacrylate sections of
} a plant root adhered to a glass coverslip, followed by extraction in
} hot acidic peroxide to remove all organic material except crystalline
} cellulose. The cellulosic cell walls left behind on the coverslip are
} nominally 100 nm thick and perhaps flattened further by the
} processing. The cell walls are present in the sample as either cross
} sections or as small regions laying in the plane of the coverslip.
} These regions can be anywhere from the size of a cell (10 x 40 or so
} microns) down to small portions thereof. All of this remaining cell
} wall is darker than the surrounding background. I am putting on a
} thin coat of Pt, somewhere around 2 nm, and imaging at 2 kV (I have
} seen the same thing at 5 kV). It really puzzles me that my sample is
} darker than background. Any explanations???
}
} THanks,
} Tobias

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Feb 24 17:34:01 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Thu, 24 Feb 2000 10:43:06 -0700 (MST)
Subject: Re: Sudan Black staining and fixing

Contents Retrieved from Microscopy Listserver Archives
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Dear Toby,

You have a basic problem - free lipids interfere with the polymerization
of epoxies. Since you are having mixed results with osmium not all lipids
may be fixing adequately. If possible, try keeping the specimens in
osmium overnight, on a rotator. (After the first hour of exposure to
osmium, change to fresh osmium). I have done this very successfully with
lipid rich structures. Hope it works.

Hildy Crowley
University of Denver
Denver, CO



From daemon Thu Feb 24 17:34:01 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Thu, 24 Feb 2000 10:53:58 -0700 (MST)
Subject: Re: Immuno-labeling of glutaraldehyd,

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 23 Feb 2000, Dr. Manfred Rohde wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopist,
}
} has anyone out there any experiences with immuno-labeling of osmificated and
} Spurr embedded samples. We have tried out etching, oxidizing sections with
} hydrogen peroxide (10% for 15 min) or sodium methylat (1% for 15 min)
} without any significant increase of label intensity (Ab used is polyclonal
} from rabbit, protein A purified)
}
} Any suggestions are welcome. Manfred
}
}
}
Hello,

You may have a problem which you do not recognize. Spurr's really has no
place in immunocytochemistry. It is highly crosslinked. As you might
know, epoxies not only crosslink, they also chemically combine with tissue
proteins making them unavailable. Also, a highly stable, highly
crosslinked embeddding medium will cut a shiny, very smooth surface on
sections. The surface relief of such a section is extremely low, and, of
course, the more surface relief, the more chance for the antigen to be
exposed to markers. You might also have a paucity of antigen which
compounds all problems.

If you want to stay with epoxies, I would suggest switching to a soft
Epon-Araldite for starters. If that does not work, then my suggestion is
to go to LR Gold. A block of LR Gold does not "cut", but cleaves, causes
something like a triple increase in surface relief thus giving more
antigens the chance to be exposed.

If I am not making myself clear, please e-mail me. I have just gone
through 2yrs of frustration with stuff like this, but this week the paper
is going out!!!!

Bye,
Hildy Crowley
University of Denver
Denver, CO

P.S. I am not making all this up. I can reference everything, but do not
have the time to do that in this format



From daemon Thu Feb 24 17:34:04 2000



From: David Hoyle :      dhoyle-at-istar.ca
Date: Thu, 24 Feb 2000 13:22:52 -0800
Subject: Cooling water/glycol Danger

Contents Retrieved from Microscopy Listserver Archives
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Before adding glycol to your Haskris water chiller check with the
manufacture of the microscope. If you use glycol to cool a Hitachi
microscope you will damage the plastic water lines and they will all need
to be replaced after a few years. I have seen this happen a few times now
after people have used this advice from the list server. Please check with
your service engineer first.

To those users of Hitachi S2500/S570, glycol is used to cool the objective
lense but only on these 2 instruments.

I am a service engineer for Hitachi in Canada and the above opinion is my
opinion and not that of Hitachi.


David Hoyle
Nissei Sangyo Canada
http://www.nsctoronto.com/
service-at-nsctoroto.com (general mail)
dhoyle-at-istar.com (direct mail)



From daemon Thu Feb 24 17:34:04 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Thu, 24 Feb 2000 12:24:27 -0600 (CST)
Subject: RE: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
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On Thu, 24 Feb 2000, Russell E. Cook wrote:

} I quote here recommendations from the Haskris Co. (we have several Haskris
} chillers):
}
} 1. If algae is present, flush the system with one of two alternatives: add
} 1 cup of chlorine bleach or one pint of hydrogen peroxide per 15 gallons of
} water. Circulate 20-30 minutes. Drain the system and refill with clean
} water.
}
} 2. To prevent regrowth of algae, one of the following additives are
} recommended:
} a. 10% solution of lab grade (no additives) ethylene glycol.
} b. Dichlorophene. This is an insoluble white powder which is a
} fungicide/bacteriacide. It should be sprinkled evenly across the entire
} surface of the water in the chiller's tank.
} c. 8 parts per million chlorine.
}
} ----------------------------------------------------------------------
} Russell E. Cook, Ph.D.
} Electron Microscopy Center for Materials Research
} Argonne National Laboratory
} Materials Science Division, Building 212
} 9700 South Cass Avenue
} Argonne, IL 60439-4838
} (630)252-7194
} FAX: (630)252-4289
} recook-at-anl.gov
}
}


I've been using dichlorophene in our Haskris chillers following the
incident related below. If you want to buy some, dichlorophene is listed
in several chemical company catalogs as:

2,2'-Methylenebis(4-chlorophenol)

Considering how I was planning to use it, I bought technical grade (90%),
since it was substantially less expensive than higher grades and have not
experienced any problems.

A complete replacement of our chiller water (along with the water in the
scope and lines) was required about a year ago when the water pump in our
TEM Haskris had to be replaced. Our sevice engineer flushed the system
using the concentrated hydrogen peroxide as mentioned above, and a truly
amazing amount of material spewed forth, so much so that small connectors
to the power supply clogged up a few weeks down the line, causing our HV
to shut down. Cleaning out the connectors and flushing the system again
with distilled water took care of the problem.

At the time, we were cautioned (by Haskris, I think) to use distilled
water and not deionized water in the chillers. Does anyone know why?

Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Thu Feb 24 17:34:06 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Thu, 24 Feb 2000 13:49:37 -0500
Subject: Re: Cooling water/glycol Danger

Contents Retrieved from Microscopy Listserver Archives
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OK, now I am concerned.

What is the real answer? When we had our Hitachi S-3200N installed
two years ago, the service engineer used an ethylene glycol/water
solution in the Haskris chiller. And it was pretty strong, too.
Something like 1:3 glycol:water.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Thu Feb 24 17:34:08 2000



From: Christensen, Kim :      ChristeK-at-whiteoaksemi.com
Date: Thu, 24 Feb 2000 14:38:10 -0500
Subject: Job Opening: TEM Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




TEM Technician
A position is available at White Oak Semiconductor, Richmond VA, USA. The
successful candidate will perform TEM operation and sample preparation in
support of DRAM fabrication and TEM engineering. The candidate should have
at least an associates degree and a minimum of 3 years experience in the
semiconductor industry; of which at least 1 year should pertain to
semiconductor TEM sample preparation. Additionally, they should understand
the fundamental tools and techniques of TEM sample preparation and the
semiconductor process from a top down and cross sectional perspective. Good
eye-hand coordination, communication skills, and attention to detail are
required. Candidates should be highly motivated, self directed, good at
problem solving, interested in learning new skills and effective working
alone or in a team environment.
Interested candidates please contact

Kim Christensen
Failure Analysis Laboratory
White Oak Semiconductor
6000 Technology Boulevard
Sandston, Virginia 23150
Ph: 804 952 7307
Fax: 804 952 7902
Pager: 1 800 759 8888, pin# 130 3382



From daemon Thu Feb 24 17:34:12 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 25 Feb 2000 07:56:59 +1100
Subject: epoxy embedding of rat eye

Contents Retrieved from Microscopy Listserver Archives
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Sandy,

If you can puncture the eye, or preferably take off the anterior
chamber, so that the chemicals can get in, there is absolutely no
problem. Standard embedding, using longish times, works well. For
instance, 3 hrs in osmium, 1 hr in UAc, 20 mins in each of the
alcohols/acetone, 1 hr in resin/acetone (dilute), overnight
infiltration in resin/acetone (more concentrated), 1 hr in warm
resin, embed. If you need to have the eye unpunctured, it may be
difficult. I've never embedded a discrete eye, but I do know that it
is really difficult to get even isolated sclera well embedded, as it
seems to act as a very effective barrier to resin. The eye is a well
designed ball that doesn't want to deflate, but that also means
nothing much will get in.

Contact me if you want further info. I've embedded more eyes than I
care to remember, including some rats eyes.

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Thu Feb 24 17:34:16 2000



From: David Hoyle :      dhoyle-at-istar.ca
Date: Thu, 24 Feb 2000 16:15:16 -0800
Subject: Cooling water/glycol Danger

Contents Retrieved from Microscopy Listserver Archives
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Carl,

You are correct Carl you can use glycol to cool your sem(all Hitachi sems)
as only the diffusion pump is being cooled and Hitachi uses a vinyl hose
that isn't effected by the Glycol. For those that have Hitachi TEM's the
water lines for the lenses and diffusion pumps are the smaller black
appearing lines, these will be adversely effected by the glycol.

Sorry for the confusion I will be more specific in the future. So in the
sem's that use the clear reinforced vinyl hoses only, glycol is okay. In
Hitachi TEM which use a small black plastic hose for the lenses and
diffusion pumps, do not use glycol.



} OK, now I am concerned.

} What is the real answer? When we had our Hitachi S-3200N installed
} two years ago, the service engineer used an ethylene glycol/water
} solution in the Haskris chiller. And it was pretty strong, too.
} Something like 1:3 glycol:water.

David Hoyle
Nissei Sangyo Canada
http://www.nsctoronto.com/
service-at-nsctoroto.com (general mail)
dhoyle-at-istar.com (direct mail)



From daemon Thu Feb 24 17:34:17 2000



From: Brian Wajdyk :      r49655-at-email.sps.mot.com
Date: Thu, 24 Feb 2000 14:39:06 -0700
Subject: Job announcement: TEM technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am pleased to bring to your attention the following TEM technician
position at Motorola's Process and Materials Characterization Laboratory
(PMCL) I encourage all persons interested to respond ASAP.

Position: TEM Technician/S9001P
Employer: Motorola-Semiconductor Products Sector's Process and Materials
Characterization Laboratory (PMCL)
Location: Mesa, AZ
Employment type: Full-time
Employment status: Full-employee (non-contractor)
Number of Positions: 2
Shift: all (compressed)
Relocation: Available

Duties/Responsibilities: Experience in Transmission Electron Microscopy
techniques including basic operation, specimen preparation, maintenance,
and troubleshooting of TEM's. Work effectively as a team player in
multiple projects providing routine and non-routine TEM analysis in
support of semiconductor product manufacturing. Exposure to many
different types of processes and technologies, and working knowledge of
FIB and EDS are a plus.

Specific knowledge: AA degree preferred. A higher or lower
classification will be established depending upon qualification and
experience.

Contact info: Send resume or questions via email to
Brian_Wajdyk-at-email.mot.com or fax to 480-655-4316 C/O Brian Wajdyk.
--
********************************************************************
Brian Wajdyk
Team Leader / Electron Microscopist (FESEM, EDS, SAM)
Motorola - Process and Materials Characterization Laboratory (PMCL)
2200 W. Broadway Rd., Mesa AZ 85202 Mail Drop: M360
Tel: 480-655-4337 Fax: 480-655-4316
Email: brian_wajdyk-at-email.mot.com Pager: 1-800-313-5960
********************************************************************



From daemon Thu Feb 24 17:34:18 2000



From: DakruegerMN1-at-aol.com
Date: Thu, 24 Feb 2000 16:49:33 EST
Subject: image analysis of fibers

Contents Retrieved from Microscopy Listserver Archives
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Image analysis folks,

I am interested in doing some PC based image analysis of fibers and fiber
properties on crimping. Is there anyone doing IA of crimp angle/amplitude or
crimps per inch? Is there commercial IA software that will do these types of
measurements?

Any help would be appreciated. Thanks.

Duane Krueger
7635 Harold Avenue
Golden Valley, Minnesota 55427

612-541-9880 (FAX)
DakruegerMN1-at-aol.com
yanktonson2-at-aol.com



From daemon Thu Feb 24 17:34:21 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 24 Feb 2000 18:00:36 -0500
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
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Gordon Couger wrote:

} Glycols won't have near the heat capacity of water. I doubt
} you will find a bug that will live in ethylene glycol but you can
} probably find many that can live in propylene glycol.
}

Dear Gordon,
I've been trying to look up the heat capacity of glycols
in my Handbook of Chemistry and Physics. It doesn't have the
data I want, but it does have the Cp for water (~4 j/gK), butane
(~0.006 j/molK = ~10^-4 j/gK, if I'm reading my cal's and
Cal's right), and 1-propanol (~0.01 j/molK with the same caviat).
} From these, I'd guess Cp for ethylene glycol is ~0.014 j/molK,
which is, indeed, much less than that of water. Propylene
glycol's Cp should be similar.
Yours,
Bill Tivol



From daemon Thu Feb 24 17:34:22 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 24 Feb 2000 18:08:15 -0500
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Divakar R wrote:

} A related question from India. We have been using DM (demineralised) water in our JEOL 2000 EX II cooled by a Neslab chiller for the last ten years. This requires change every 6 months approximately. What additives can be used to prevent algae (? it is green and slimy and I am not a biologist) growth? Something obtainable locally (chemical name rather than trade names) will be most useful to me. Thanks very much.

Dear Divakar,
Russell's suggestion of dichlorophene is good--it's also
what we use. To prevent growth in the tubing, we added some
CuSO4--being careful to keep the pH slightly above 7.5.
We have copper tubing in the system, so the Cu++ should not
be a problem, and it will inhibit algal growth. At that pH,
CuSO4 is not too soluble, so go slowly (if at all).
Yours,
Bill Tivol



From daemon Thu Feb 24 17:34:23 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 24 Feb 2000 18:15:47 -0500
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Heather A Owen wrote:

Dear Heather,


} A complete replacement of our chiller water (along with the water in the
} scope and lines) was required about a year ago when the water pump in our
} TEM Haskris had to be replaced. Our sevice engineer flushed the system
} using the concentrated hydrogen peroxide as mentioned above, and a truly
} amazing amount of material spewed forth, so much so that small connectors
} to the power supply clogged up a few weeks down the line, causing our HV
} to shut down. Cleaning out the connectors and flushing the system again
} with distilled water took care of the problem.
}
}

We added inline filters--the ones with the fiber cartridges--to
trap all the particulate material in the lines, and over a period of several
months, we probably got as much material as you got all at once. I highly
recommend adding filters.

At the time, we were cautioned (by Haskris, I think) to use distilled

} water and not deionized water in the chillers. Does anyone know why?
}

Hard to say. Deionized water can contain material leached out
of the resin used, and maybe that is bad for the system, while volatiles
(mostly organics, I'd guess) from distillation may not be.
Yours,
Bill Tivol



From daemon Thu Feb 24 17:44:31 2000



From: Andrew McNaughton :      andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Fri, 25 Feb 2000 12:31:14 +1300
Subject: Reference points in serial sections.

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Hello

A student wishes to take a number of serial sections through a bee brain
mounted in glycerine. The total distance is around 500µm and she would
like to include three reference points which enable the sections to be
correctly orientated throughout the brain. Embedding three small rods
seems to be the most likely option but the material would need to be stable
and able to be cut by a vibratome. Keeping the rods parallel would also be
beneficial.

If anyone has tried this sort of thing or has any suggestions as to what we
could use for the markers I'd appreciate hearing your bright ideas.

Regards

Andrew McNaughton

______________________________________________________________________________
Andrew McNaughton
South Campus Electron Microscope Unit
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

e-mail: andrew.mcnaughton-at-stonebow.otago.ac.nz
______________________________________________________________________________ht




From daemon Thu Feb 24 21:00:05 2000



From: HŒkan Olin :      hakan.olin-at-fy.chalmers.se
Date: Fri, 25 Feb 2000 08:05:38 +0100
Subject: SPM information on the Web - including TEM-STM

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Dear Timothy,

We are using sputter coater in "etch" mode for this purpose. It is doing the
job flawlessly.
I don't know about your coater but I'll give you the settings we are using
here.
The coater has maximum operating voltage of 1400V. The grids are put on
glass plate (so they do not make contact with the lower electrode) and then
the plate is put on the lower electrode. The current we use is 5 mA for 20
seconds (the current depends strongly on the pressure and the voltage). The
pressure is about 20 Pa.

Good luck !

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Timothy Schneider {Timothy.Schneider-at-mail.tju.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 24, 2000 4:29 AM


Dear Microscopy users,

I just want to inform you about a new web site
(http://www.nanofactory.com) with some useful information on scanning
probe microscopy (SPM)

There are, for example:
- Weekly update of SPM research articles
- Suggestions of some SPM literature
- Links to many SPM research groups
- Links to almost all SPM companies

There are also some interesting mpeg-movies with TEM-STM measurements of
two tunneling tips jumping into each other.

Best regards,
Hakan Olin
--
Dr Hakan Olin
Physics and Engineering Physics
Chalmers University of Technology
SE-412 96 Goteborg
Sweden
tel. +46-31-772 3338
fax +46-31-772 3367
mobile +46-70-30 88 99 0
e-mail hakan.olin-at-fy.chalmers.se
home page http://fy.chalmers.se/~f4aolin



From daemon Fri Feb 25 08:32:45 2000



From: Wim Jacob :      jacob-at-uia.ua.ac.be
Date: Fri, 25 Feb 2000 11:31:08 +0100
Subject: Re: Food Science Prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An effective way to prevent your cooling system from growing
green or blue-green algae is to ensure that it is totally light-tight.
Algae require light for photosynthesis. The Philips engineers who
installed our CM120 Biotwin recommended we connect up the
chiller with the black-lined vinyl hose used in drinks vending
machines, where microorganism growth in the water lines is
obviously undesirable. The product we used is trademarked
"Aquavend", is opaque white outside and black inside. It appears to
have worked so far, though the ethylene glycol we use would also
discourage algae.
Yours
Chris Jeffree

Date sent: Thu, 24 Feb 2000 18:08:15 -0500
} From: William Tivol {tivol-at-wadsworth.org}
To: microscopy-at-sparc5.microscopy.com


Dear Debby,
Some ten yaers ago we were doing research on sugar syrup in cooperation with a sugar company. They were interested in the stucture of mixtures of sugar syrups. W e had some succes using freeze-fracture and looking at the replicas in the TEM. Of course these were not mixtures of cereals with sugar syrup and the specimens were allowed to be a lot smaller but this could be a suggestion.
Succes !
Wim Jacob
Ctr.of Electron Microscopy
University of Antwerp
Belgium

Debby Sherman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
} Well I guess we never run out of new challenges when it comes to specimen prep. This one concerns a product that is made of a cereal bound together with a sugar syrup. The investigators want to see how the syrup behaves as to structural integrety over a period of time with and without the addition of various stabilizing components. Information would be collected at the light microscopic level.
} We first thought of cryosectioning followed by examination using LM. However, the product is filled with air pockets and literally disintegrates when microtomed. It is easily cut into pieces at temperatures above freezing as long as the pieces are no thinner than3-4 mmAccempts to infiltrate it with OCT compoound prior to freezing were not satisfactory.
} New thought is to use a low temperature resin to infiltrate the sample followed by UV or chemical polymerization. butThe resulting blocks could then be microtomed at room temperature and imaged. It would be desirable to cut sections of 4-8 µm in thickness which is too thick for an ultramicrotome with glass or diamond knives. Also UV polymerization would probably limit us to very small sample size which may be a problem.
} Any suggestions would be appreciated as to resins to use which have low viscosity, could be used to infiltrate pieces of ~0.5-1cm sq, polymerize at low temperatures (+4 to 0oC range) using UV or chemical polymerization, and are preferably soft enough to section with a metal microtome blade. Any other suggestions of preparations techniques to try would be very appreciated.
}
} Thanks in advance for your input.
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Microscopy Center in Agriculture FAX: 765-494-5896
} Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
} Purdue University
} 1057 Whistler Building
} West Lafayette, IN 47907-1057



From daemon Fri Feb 25 08:32:47 2000



From: Wim Jacob :      jacob-at-uia.ua.ac.be
Date: Fri, 25 Feb 2000 11:52:17 +0100
Subject: Re: Can EELS, WDS or FT-IR localize As in Biol. tissues?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Doug,
Why don't you try imaging SIMS? I would not surprised if such instrument(s) are
present at the University of Arizona.
Of course this would restrict the resolution, I gess, to 0.1 - 0.5 micrometer,
with the newer instruments.
Regards
Wim Jacob
Ctr. of Electron Microscopy
University of Antwerp
Belgium

Doug Cromey wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Colleagues,
}
} We are interested in localizing arsenic in biological tissues (toxicology
} research), but have been unable to come up with a technique that will give
} us the cellular localization information we're looking for (sub-cellular
} would be even better).
}
} We have tried TEM with energy dispersive x-ray microanalysis and our
} concentrations are apparently too low, so we are unable to detect arsenic.
}
} A few years ago we looked into using the autometallographic techniques that
} have been published by Dr. Gorm Dansher & his colleagues. We were told
} that the photographic emulsions required were no longer available.
}
} In a brainstorming session yesterday several techniques were bantered
} about, but in truth we are not familiar enough with the techniques to know
} if they would suit our needs. Does EELS have anything to offer, or perhaps
} wavelength dispersive spectoscopy (WDS)? How about some of the new FT-IR
} instruments? Are there any other techniques that might be able to localize
} arsenic and/or indicate its state of oxidation?
}
} Vendors are welcome to reply.
}
} Yours,
} Doug Cromey
} ....................................................................
} : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
} : Research Specialist, Principal University of Arizona :
} : (office: AHSC 4212A) P.O. Box 245044 :
} : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
} : (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
} :...................................................................:
} http://www.pharmacy.arizona.edu/exp_path.html
} Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Fri Feb 25 18:05:15 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Fri, 25 Feb 2000 15:56:01 +0100
Subject: TEM: a new TEM website.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

Please visit my new homepage at http://syli.homepage.com at your convinience!
It contains not only my current research work, but also LINKS to useful journal informations, instruction for authors, on-line EELS database, periodic table, physical constants, online TEM course and etc.

I am looking forward to your invaluable suggestions!
Yours sincerely,

Shu-You Li


**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************






From daemon Fri Feb 25 18:05:20 2000



From: Jill Verlander :      verlaj-at-medicine.ufl.edu
Date: Fri, 25 Feb 2000 14:25:57 -0500
Subject: Immunogold Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Becky,
I do not know if it is okay in the S4700 to use glycol. If it is working
as in why change?


X-From_: r-holdford-at-ti.com Thu Feb 24 19:53:24 2000


X-From_: Patrick.Kilcran-at-hii-hitachi.com Thu Feb 24 23:55:28 2000
} From: "Kilcran, Patrick-HIIEX" {Patrick.Kilcran-at-hii-hitachi.com}
To: dhoyle-at-istar.ca
Cc: "Lima, Rick-HIIEX" {Rick.Lima-at-hii-hitachi.com}


Dear Researcher:

The University of Florida College of Medicine Electron Microscopy Facility
is hosting a two-day workshop on immunogold technique. The workshop will
include two identical sessions on April 10/11 and 13/14. Dr. Jan
Leunissen from the Aurion Immunogold Reagent & Accessories, an
internationally known expert in the field, will be the instructor for the
workshop. Attached is the program information. If you are interested in
attending this workshop, please contact Dr. Verlander or Ms. Hong Yi at
the addresses provided below. Due to practical considerations, the
workshop will be limited to a maximum of 20 participants for each session.
Therefore, we encourage you to register as soon as possible. If you are
not able to attend, we would appreciate if you could pass the message onto
someone else who might be interested in learning about immunogold
techniques. Thank you very much.

Dr. Jill W. Verlander Reed (workshop faculty)
Director, University of Florida College of Medicine Electron Microscopy
Facility Phone: (352) 846-0820 Fax: (352) 846-3299 verlaj-at-medicine.ufl.edu

Hong Yi (technical coordinator)
Supervisor, Emory Neurology Microscopy Core Laboratory
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu


University of Florida, College of Medicine, Electron Microscopy Facility
Aurion Immunogold Reagents & Accessories / Electron Microscopy Sciences

Present
Immunogold Workshop

April 10-14, 2000
Gainesville, FL

WORKSHOP HOST
Dr. Jill Verlander Reed
Director, Electron Microscopy Facility
University of Florida College of Medicine
Phone: (352) 846-0820
Fax: (352) 846-3299
verlaj-at-medicine.ufl.edu

WORKSHOP INSTRUCTORS

Dr. Jan L.M. Leunissen is an internationally known scientist in the field
of ultrastructural localization. He received his Ph.D in molecular cell
biology at the University of Utrecht in the Netherlands. Dr. Leunissen is
the inventor of ultra small colloidal gold probes and also the founder and
president of the company Aurion, which specializes in immunogold reagents
and custom immunogold labeling. He has been invited to many international
microscopy conferences and workshops and is especially experienced in
providing hands-on training. His previous workshops in Europe, Asia, and
the US have been fully attended and very well received.

Dr. Jill W. Verlander is the Director of the Electron Microscopy Facility
for the University of Florida College of Medicine. Dr. Verlander has 15
years of experience in ultrastructural research in kidney and is known
internationally for her work examining structure-function relationships in
renal transport epithelia. Her work has been published in such journals as
the Journal of Clinical Investigation, the American Journal of Physiology,
Journal of the American Society of Nephrology, and Kidney International.
These studies have been accomplished using light microscopic
immunohistochemistry and in situ hybridization, scanning and transmission
electron microscopy, morphometric analyses, and immunogold and
immunoperoxidase cytochemistry at the ultrastructural level.

GENERAL INFORMATION
Objectives
To provide researchers the opportunity to learn the theory and practice of
immunogold labeling To permit participants to process their own samples
using these techniques under expert guidance To promote technology
exchange and research collaboration

Registration Fees
Regular: $250.00
Student: * $150.00

Lodging
Sheraton Gainesville, $69/night
Phone: (352) 373-6721

Rooms will be reserved in the above hotel under the name "UF EM workshop".
Shuttle buses are available between the hotel and UF campus

Enrollment Note
Two sessions (A; April 10-11, B: April 13-14) will be hosted at UF. Each
session will be limited to a maximum of 20 participants. The workshops
will provide samples to those who prefer not to bring their own. Each
participant will be provided with a sample of gold conjugate of their
choice at the end of the workshop.

MAIN CURRICULUM
The properties of gold particles and their protein conjugates.
Theories underlying immunogold labeling protocols.
Silver enhancement of gold particles
Imunogold labeling on a variety of sample preparations for LM.
Immunogold labeling for EM
a. Pre-embedding immunogold labeling using ultra_small gold conjugates
and silver enhancement. b. Post-embedding immunogold labeling using ultra
small and conventional colloidal gold conjugates.

CONTACT PERSON AND TECHNICAL COORDINATOR
Hong Yi
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu


Jill Verlander Reed, D.V.M.
Associate Scientist
Office of the Dean
University of Florida College of Medicine
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299



From daemon Fri Feb 25 18:05:26 2000



From: george sibbald :      geos-at-goldrush.com
Date: Fri, 25 Feb 2000 13:53:49 -0700
Subject: AFM Newslatter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Molecular Imaging is updating our contact information.

If you are interested in scanning probe microscopy, would you please take a
moment and update your information at http://www.molec.com/info/form.html

Thank you

George

_______________________
George Sibbald, President
Molecular Imaging: Technology Leader In Advanced Bio-AFM Systems
9830 South 51 Street, Ste 124A
Phoenix AZ 85044
(480) 753-4311
www.molec.com




From daemon Fri Feb 25 18:05:28 2000



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Fri, 25 Feb 2000 14:03:54 -0800
Subject: Re: Glycol for cooling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In fact, we have an S-4700 and the service technician told us that there are tons of problems with using glycol in the chillers. We have a Haskris chiller. Apparently, the glycol volatilizes some of the hose components.


De Wood


At 11:01 AM 2/25/2000 -0800, David Hoyle wrote:

} ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

}

} Becky,

} I do not know if it is okay in the S4700 to use glycol. If it is working

} as in why change?

}

}

} X-From_: r-holdford-at-ti.com Thu Feb 24 19:53:24 2000

} Date: Thu, 24 Feb 2000 19:26:10 -0600

} } From: Becky Holdford { {r-holdford-at-ti.com}

} Organization: KFAB Physical Analysis Lab, Texas Instruments, Dallas, TX

} X-Mailer: Mozilla 4.51 [en] (WinNT; U)

} X-Accept-Language: en,en-US,en-GB,uk,ru

} To: David Hoyle { {dhoyle-at-istar.ca}

} Subject: Re: Cooling water/glycol Danger

} X-MIME-Autoconverted: from 8bit to quoted-printable by tower.ti.com id

} TAA19583

}

} David:Ê we have 3 Hitachi S4700's that have the final lenses water-cooled.Ê

} Does the caveat of glycol still hold?Ê We are using distilled water and have

} had no problem so far.

}

} David Hoyle wrote:

}

} } ----------------------------------------

}

}

{bold} ********************************************************************

Delilah F. Wood

Botanist

USDA-ARS-WRRC

800 Buchanan St.

Albany, CA 94710


Tel: 510-559-5653

Fax: 510-559-5818 {/bold}

{/x-rich}



From daemon Sat Feb 26 10:27:48 2000



From: gregory.argentieri-at-pharma.Novartis.com
Date: Fri, 25 Feb 2000 18:08:34 -0600
Subject: Phtographic printers for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




}
} Looking to find and to determine what is the best printer (photographic and
} archive quality)
} for TEM digital images.
}
} Currently considering Kodak 8670PS, Codonics, or Fuji Printers
} Would be interested in comments from people using photoraphic qualtiy
} printers
} for TEM applications
} thoughts, "next time's" etc.
}
} Thank You
}
} Gregory Argentieri
} Novartis Pharmacuticals Corp
} gregory.argentieri-at-pharma.novartis.com
}
}
}
}




From daemon Sat Feb 26 10:27:48 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 25 Feb 2000 18:10:57 -0600
Subject: RE: Food Science Prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby Sherman
Talk to Mark Cavaleri at 3M. He made a polished cross sectgion of M%M
candies and preserved tyhe sugr layer

Sam Purdy

} ----------
} From: Debby Sherman
} Sent: February 2000 1:17 PM
} To: message to: MSA list
} Subject: Food Science Prep.
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} Well I guess we never run out of new challenges when it comes to
} specimen prep. This one concerns a product that is made of a cereal bound
} together with a sugar syrup. The investigators want to see how the syrup
} behaves as to structural integrety over a period of time with and without
} the addition of various stabilizing components. Information would be
} collected at the light microscopic level.
} We first thought of cryosectioning followed by examination using LM.
} However, the product is filled with air pockets and literally
} disintegrates when microtomed. It is easily cut into pieces at
} temperatures above freezing as long as the pieces are no thinner than3-4
} mmAccempts to infiltrate it with OCT compoound prior to freezing were not
} satisfactory.
} New thought is to use a low temperature resin to infiltrate the sample
} followed by UV or chemical polymerization. butThe resulting blocks could
} then be microtomed at room temperature and imaged. It would be desirable
} to cut sections of 4-8 µm in thickness which is too thick for an
} ultramicrotome with glass or diamond knives. Also UV polymerization would
} probably limit us to very small sample size which may be a problem.
} Any suggestions would be appreciated as to resins to use which have low
} viscosity, could be used to infiltrate pieces of ~0.5-1cm sq, polymerize
} at low temperatures (+4 to 0oC range) using UV or chemical polymerization,
} and are preferably soft enough to section with a metal microtome blade.
} Any other suggestions of preparations techniques to try would be very
} appreciated.
}
} Thanks in advance for your input.
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Microscopy Center in Agriculture FAX: 765-494-5896
} Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
} Purdue University
} 1057 Whistler Building
} West Lafayette, IN 47907-1057
}
}




From daemon Sat Feb 26 10:27:49 2000



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Fri, 25 Feb 2000 16:10:42 -0800
Subject: Fwd: sem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I received this today. If anyone could help, please reply to the sender.

Thanks.

ML

} Date: Fri, 25 Feb 2000 17:14:04 -0600
} From: Howard Hsu {hhsu-at-kumc.edu}
} Organization: KU Medical Center
} X-Accept-Language: en
} MIME-Version: 1.0
} To: wong-at-msg.ucsf.edu
} Subject: sem
}
} I am a researcher at the University of Kansas Med. Center and would like
} study a biological sample for the presence of mineral using SEM
} approach. If you can provide external service on cost or collaborative
} basis, please let me know. Otherwise, I would greatly appreciate if you
} would let me know any academic or industrial sources that provide this
} type of service. Thank you. Howard Hsu, Ph.D.
}
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu



From daemon Sat Feb 26 10:27:49 2000



From: Ronald R. Matias :      rrm-at-skyinet.net
Date: Sat, 26 Feb 2000 08:19:49 +0800
Subject: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello and Help!

We are a hospital based research lab. starting to use the electron
microscope to identify or confirm pathogens that cause gastrointestinal
disturbances. We would like to know if someone out there could recommend
appropriate specific EM protocols (negative staining, processing of the
gastrointestinal contents, etc....) to visualize these pathogens (ranging
from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
the routine EM preparations designed to examine tissues.

In the interest of bring quality healthcare to our people, we will be very
much obliged,

ronnie matias



From daemon Sat Feb 26 10:28:10 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 26 Feb 2000 05:13:31 -0500
Subject: SEM/TEM analytical service request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mei Lie Wong wrote:
==================================================
I received this today. If anyone could help, please reply to the sender.

Thanks.

ML

} Date: Fri, 25 Feb 2000 17:14:04 -0600
} From: Howard Hsu {hhsu-at-kumc.edu}
} Organization: KU Medical Center
} X-Accept-Language: en
} MIME-Version: 1.0
} To: wong-at-msg.ucsf.edu
} Subject: sem
}
} I am a researcher at the University of Kansas Med. Center and would like
} study a biological sample for the presence of mineral using SEM
} approach. If you can provide external service on cost or collaborative
} basis, please let me know. Otherwise, I would greatly appreciate if you
} would let me know any academic or industrial sources that provide this
} type of service. Thank you. Howard Hsu, Ph.D.
}
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu
============================================================
Although you have specifically asked about SEM, I am aware only of a TEM
approach for this kind of analysis. We at Structure Probe, Inc. do perform
this kind of work for clients on a commercial basis. You can see an example
of the final sample that is analyzed by TEM on our website at page URL
http://www.2spi.com/catalog/instruments/etchers4.html

The method is pretty much described so anyone really could do with, provided
they did have access by a plasma etcher such as the SPI Plasma Prep II. If
you wanted to do it yourself and were having difficulties, let us know,
perhaps we could give you assistance. We have found that the removal of the
organics is usually quite important in order to remove the source of the
unwanted Bremsstrahlung radiation.

We have heard from persons trying to do this by SEM/EDS, where by the
section is deposited on a polished beryllium mount, and then the organics
etched away but they seem to have not been successful.

Disclaimer: SPI Supplies manufactures the plasma etching equipment needed
to practice this procedure and our Structure Probe® analytical services part
of our business performs this kind of sample preparation and analysis for
clients. We realize there might be other methods for doing this, but we
have favored the approach that works for us.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Sat Feb 26 15:00:30 2000



From: przybylo :      przybylowicz-at-nac.ac.za
Date: Sat, 26 Feb 2000 22:44:21 +0200
Subject: As in biological tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Doug,


We have some experience in using micro-PIXE (proton induced X-ray emission)
and the so-called nuclear microprobe in toxicological research. It is more
sensitive than EDS and WDS, is very easy to quantify (standardless
technique). In principle you can have detection limits of the order of 1
ppm in point analysis mode. Specimens can be of any thickness and their
actual thickness is assessed by the simultaneous use of the BS technique
(proton backscatering spectrometry). What in my opinion makes it really
attractive for this purpose is its very good scanning capability. Scan sizes
are from ca. 2.5 mm x 2.5 mm down to the beam resolution, which is of the
order of few micrometer (can be down to 1 micrometer or even less, but in
most of our uses few micrometers is good enough).
The sample preparation is similar as for EDS, using cryotechniques to avoid
elemental losses and redistribution.
We have so far done studies on Ni, Mo, Cd. The detection limits for As are
better than for Mo and Cd.
Of course, this technique cannot indicate the state of oxidation, you can
really treat it as a "more sensitive EDS".

If you want more information, I can refer you to our earlier work and
perhaps you might be interested in contacting me.

Best regards

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: przybylowicz-at-nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820
http://www.nac.ac.za/IBA/
http://www.nac.ac.za/materials/
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Sat Feb 26 15:10:25 2000



From: przybylo :      przybylowicz-at-nac.ac.za
Date: Sat, 26 Feb 2000 22:50:42 +0200
Subject: As in biological tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Doug,


We have some experience in using micro-PIXE (proton induced X-ray emission)
and the so-called nuclear microprobe in toxicological research. It is more
sensitive than EDS and WDS, is very easy to quantify. In principle you can
have detection limits of the order of 1 ppm in point analysis mode.
Specimens can be of any thickness and their actual thickness is assessed by
the simultaneous use of the BS technique (proton backscatering
spectrometry). What in my opinion makes it really attractive for this
purpose is its very good scanning capability. Scan sizes are from ca. 2.5 mm
x 2.5 mm down to the beam resolution, which is of the order of few
micrometer (can be down to 1 micrometer or even less, but in most of our
uses few micrometers is good enough).
The sample preparation is similar as for EDS, using cryotechniques to avoid
elemental losses and redistribution.
We have so far done studies on Ni, Mo, Cd. The detection limits for As are
better than for Mo and Cd.
Of course, this technique cannot indicate the state of oxidation, you can
really treat it as a "more sensitive EDS".

If you want more information, I can refer you to our earlier work and
perhaps you might be interested in contacting me.

Best regards

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: przybylowicz-at-nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820
http://www.nac.ac.za/IBA/
http://www.nac.ac.za/materials/
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Mon Feb 28 07:33:33 2000



From: John Catino :      jwcatino-at-concentric.net
Date: Sun, 27 Feb 2000 21:34:39 -0500
Subject: Print Processor for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Kodak Royal Print Processor for sale. The unit is in good
working condition. Our lab is going digital.

Please contact me via e-mail if interested.

John





From daemon Mon Feb 28 07:33:33 2000



From: John Catino :      jwcatino-at-concentric.net
Date: Sun, 27 Feb 2000 21:25:36 -0500
Subject: Kodak Royal Print Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Kodak Royal Print Processor for sale. The unit is in good
working condition. Our lab is going digital.

Please contact me via e-mail if interested.

John



From daemon Mon Feb 28 07:33:48 2000



From: Hans-Werner Liebsch :      hwl.bioanalytic-at-bluewin.de
Date: Mon, 28 Feb 2000 11:22:52 +0100
Subject: Active Vibration Isolation News

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An information about a new principle in vibration damping to all
microscopy users, who have problems with particularly low frequency
vibrations from natural environment.

I invite you kindly to visit our home page:
http://www.hwlbioanalytic.com

We show there active vibration isolation systems for various
applications with damping frequencies from 0,6 Hz to 100/200 Hz and load
capacities (depending from system) from max. 90 kg up to 330/660 kg and
max. 1.500 kg.
Thank you for your time.

With kind regards
Hans-Werner Liebsch

--------------------------------------------
HWLbioanalyticSYSTEMS
Hans-Werner Liebsch
Sales Office Scient. Instruments
Weidenstrasse 11
D - 72119 ENTRINGEN
Germany
Tel.: 0049 (0) 7073 916796
Fax : 0049 (0) 7073 916798
e-mail : hwl.bioanalytic-at-bluewin.de
http://www.hwlbioanalytic.com




From daemon Mon Feb 28 08:33:52 2000



From: tracy gales :      tl_gales-at-fccc.edu
Date: Mon, 28 Feb 2000 09:24:29 -0500
Subject: Virology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ronnie,

We use many of the techniques and the atlas from the following excellent
book.
"EM in Diagnostic Virology" by Doan and Anderson,1987, Cambridge
University Press,
ISBN 0 521 24311 4.

--
Tracy Gales
Fox Chase Cancer Center
Electron Microscopy Facility
7701 Burholme Ave.
Philadelphia PA 19111
Tel. 215-728-2484
Fax 215-728-2412




From daemon Mon Feb 28 10:40:21 2000



From: Louis M Kerr :      lkerr-at-mbl.edu
Date: Mon, 28 Feb 2000 11:12:56 -0500
Subject: TEM Diffraction plate reader

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A friend recently retired and gave me a TEM diffraction plate reader. This
was made by Ernest F. Fullam, Inc. in the early 1970's. It is still in the
wooden shipping crate, is in unused condition and looks complete except
there is no manual. It is a basic no-frills manual version. It has a high
quality Starrett vernier scale and could be used for measurement of any
electron micrograph, or other type of transparency, or it may be useful for
teaching the history and theory of diffraction pattern measurement.

I would be willing to send it to someone willing to pay the shipping costs.

Thanks,
Louie

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu




From daemon Mon Feb 28 19:06:50 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Mon, 28 Feb 2000 12:49:27 -0500 (EST)
Subject: Position Available (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





RESEARCH ASSOCIATE

Electron Microscopy Facility

University of Kentucky


The University of Kentucky invites applications for a Research Associate
in the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286



***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

Director, Electron Microscopy Facility

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/





From daemon Mon Feb 28 19:06:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 12:38:41 -0500 (EST)
Subject: Re: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ronnie,

We perform about 1000 exams/yr for viruses using EM. Below are some refs
(check out especially 2, 5, 9, 11, 14). Also, at the bottom is a
synopsis I give to our medical students; maybe it will be useful.

As for enteric viruses, EM is the best way to look for them. Many of
them do not grow in tissue culture, and those that can be coaxed, do so
under special conditions that are not employed in the routine culture
lab. Biochemical identification (e.g., immunological kits, PCR, Western
Blots) require a specific reagent to recognize the virus and if you
don't use the right reagent, you will miss viruses (e.g., if you run a
test for rotavirus, you will miss adenovirus, etc.) Furthermore, there
aren't biochemical reagents for all viruses. With EM, you can
see a wide variety of viruses by doing a negative stain of an aqueous
extract of stool.

Good luck,
Sara

REFERENCES ON VIRUS IDENTIFICATION BY ELECTRON MICROSCOPY

1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses
associated with HIV infection in Tanzanian children with chronic
diarrhea. Ped AIDS HIV Inf 5:296-299.

2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic
Virology: A Practical Guide and Atlas, Cambridge University Press, New York.

3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE,
Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro
LS, and Jaqua M-J. 1990. Six year retrospective surveillance of
gastroenteritis viruses identified at ten electron microscopy centers in
the United States and Canada Pediatr Infect Dis J 9:709-714.

4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus
Infections, CRC Press, Boca Raton, FL.

5. Miller SE. 1986. Detection and identification of viruses by
electron microscopy. J Electron Microsc Tech 4:265-301.

5. Miller SE, Howell DN. 1988. Viral infections in the acquired
immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.

7. Miller SE. 1988. Diagnostic virology by electron microscopy.
ASM News 54:475-481.

8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis.
EMSA Bull 19:53-59.

9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and
Methods. McGraw-Hill, New York.

10. Miller SE. 1991. Evaluation of electron microscopic information
available from clinical samples. In de la Maza LM, Peterson EM (eds.),
Medical Virology, Vol 10. Irvine, CA. pp 21-54.

11. Miller SE. 1995. Diagnosis of viral infection by electron
microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc, Washington, DC. pp 35-76.

12. Oshiro LS, Miller SE. 1992. Application of electron microscopy
to the diagnosis of viral infections. In Lennette EH (ed), Laboratory
Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.

13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC
Press, Boca Raton, FL.

14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral
Diagnosis, CRC Press, Boca Raton, FL.

15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP
Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New York.

2, 5, 9, 11-14 = Methods and micrographs
2, 14 = Excellent atlases
7, 8 = Brief synopses of technique and basis of virus ID
15 = Good general reference, but don't memorize!! Lists all viruses, not
just human.



TWO WAYS TO LOOK AT VIRUSES BY EM:
Negative staining
Used with liquid samples
Shows whole virus (size, surface structure important)
Thin sectioning
Used with cells and tissues
Shows a slice of an infected cell
Shows relationship of viruses to cell organelles (internal
structure and cell organelle association important)
Location within the cell is a clue to nucleic acid type
DNA viruses are usually seen in the nucleus (some
exceptions).
RNA viruses are usually seen in the cytoplasm
(some exceptions).
Exceptions:
Naked viruses get out of the cell by lysis; if
the cell is very sick, there may be virions
everywhere, e.g., adenovirus (DNA).
Poxviruses are DNA viruses, but are constructed
in the cytoplasm.
Hepatitis B core particles (DNA) may be in the
nucleus and cytoplasm. Dane particles
(complete viruses) are only cytoplasmic.
Some paramyxovirus (RNA) nucleocapsids, not the
complete virion, can be seen in the nucleus
(e.g., measlesvirus).
Enveloped viruses are associated with cell membranes; the
type of cell membrane (plasma membrane,
nuclear membrane, endoplasmic reticulum, vacuoles) can
be a clue to virus identification.

SUMMARY OF VIRUS TYPES (based on morphology):
Naked viruses
All human naked viruses are icosahedral (spherical).
There are 3 size ranges from 20-35 nm, 45-55 nm, 70-90 nm. Examples:
Small: parvovirus, enterovirus, rhinovirus
Medium: polyomavirus, papillomavirus
Large: rotavirus/reovirus, adenovirus
Enveloped viruses
All have a lipoprotein membrane on the outside of the
nucleocapsid. Most derive this layer by budding through
cell membranes; this pliable layer makes the virion
pleomorphic. Poxviruses synthesize their shell de novo;
sometimes they pick up an extra covering by budding. They are
not pleomorphic, but different varieties may be more ovoid,
others, brick-shaped.
Most range in size from 40-400 nm. (Filoviruses are 80 nm by up
to 1400 nm.)
The envelope has proteins on the outside that may look like
spikes or fuzz (e.g., influenza virus, coronavirus).
Sometimes the spikes are too short to be distinguished by EM,
making the virion appear smooth (e.g., herpesviruses,
rubella virus).
The nucleocapsid shape can be
a. icosahedral, like the naked viruses; however, some
are morphologically nondescript as seen by EM;
b. filamentous, like a "slinky."
c. complex (poxvirus cores look like dumbbells with
lateral bodies)
d. morphologically nondescript (just dense without any
particular shape).
Enveloped DNA viruses (except pox) construct their nucleocapsids
in the nucleus.
Enveloped RNA viruses construct their nucleocapsids in the
cytoplasm; (measles nucleocapsids can sometimes be seen
in the nucleus).



On Sat, 26 Feb 2000, Ronald R. Matias wrote:

} Date: Sat, 26 Feb 2000 08:19:49 +0800
} From: Ronald R. Matias {rrm-at-skyinet.net}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need help on EM negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello and Help!
}
} We are a hospital based research lab. starting to use the electron
} microscope to identify or confirm pathogens that cause gastrointestinal
} disturbances. We would like to know if someone out there could recommend
} appropriate specific EM protocols (negative staining, processing of the
} gastrointestinal contents, etc....) to visualize these pathogens (ranging
} from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
} the routine EM preparations designed to examine tissues.
}
} In the interest of bring quality healthcare to our people, we will be very
} much obliged,
}
} ronnie matias
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Mon Feb 28 19:06:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 12:38:41 -0500 (EST)
Subject: Re: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------


Ronnie,

We perform about 1000 exams/yr for viruses using EM. Below are some refs
(check out especially 2, 5, 9, 11, 14). Also, at the bottom is a
synopsis I give to our medical students; maybe it will be useful.

As for enteric viruses, EM is the best way to look for them. Many of
them do not grow in tissue culture, and those that can be coaxed, do so
under special conditions that are not employed in the routine culture
lab. Biochemical identification (e.g., immunological kits, PCR, Western
Blots) require a specific reagent to recognize the virus and if you
don't use the right reagent, you will miss viruses (e.g., if you run a
test for rotavirus, you will miss adenovirus, etc.) Furthermore, there
aren't biochemical reagents for all viruses. With EM, you can
see a wide variety of viruses by doing a negative stain of an aqueous
extract of stool.

Good luck,
Sara

REFERENCES ON VIRUS IDENTIFICATION BY ELECTRON MICROSCOPY

1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses
associated with HIV infection in Tanzanian children with chronic
diarrhea. Ped AIDS HIV Inf 5:296-299.

2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic
Virology: A Practical Guide and Atlas, Cambridge University Press, New York.

3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE,
Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro
LS, and Jaqua M-J. 1990. Six year retrospective surveillance of
gastroenteritis viruses identified at ten electron microscopy centers in
the United States and Canada Pediatr Infect Dis J 9:709-714.

4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus
Infections, CRC Press, Boca Raton, FL.

5. Miller SE. 1986. Detection and identification of viruses by
electron microscopy. J Electron Microsc Tech 4:265-301.

5. Miller SE, Howell DN. 1988. Viral infections in the acquired
immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.

7. Miller SE. 1988. Diagnostic virology by electron microscopy.
ASM News 54:475-481.

8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis.
EMSA Bull 19:53-59.

9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and
Methods. McGraw-Hill, New York.

10. Miller SE. 1991. Evaluation of electron microscopic information
available from clinical samples. In de la Maza LM, Peterson EM (eds.),
Medical Virology, Vol 10. Irvine, CA. pp 21-54.

11. Miller SE. 1995. Diagnosis of viral infection by electron
microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc, Washington, DC. pp 35-76.

12. Oshiro LS, Miller SE. 1992. Application of electron microscopy
to the diagnosis of viral infections. In Lennette EH (ed), Laboratory
Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.

13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC
Press, Boca Raton, FL.

14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral
Diagnosis, CRC Press, Boca Raton, FL.

15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP
Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New York.

2, 5, 9, 11-14 = Methods and micrographs
2, 14 = Excellent atlases
7, 8 = Brief synopses of technique and basis of virus ID
15 = Good general reference, but don't memorize!! Lists all viruses, not
just human.



TWO WAYS TO LOOK AT VIRUSES BY EM:
Negative staining
Used with liquid samples
Shows whole virus (size, surface structure important)
Thin sectioning
Used with cells and tissues
Shows a slice of an infected cell
Shows relationship of viruses to cell organelles (internal
structure and cell organelle association important)
Location within the cell is a clue to nucleic acid type
DNA viruses are usually seen in the nucleus (some
exceptions).
RNA viruses are usually seen in the cytoplasm
(some exceptions).
Exceptions:
Naked viruses get out of the cell by lysis; if
the cell is very sick, there may be virions
everywhere, e.g., adenovirus (DNA).
Poxviruses are DNA viruses, but are constructed
in the cytoplasm.
Hepatitis B core particles (DNA) may be in the
nucleus and cytoplasm. Dane particles
(complete viruses) are only cytoplasmic.
Some paramyxovirus (RNA) nucleocapsids, not the
complete virion, can be seen in the nucleus
(e.g., measlesvirus).
Enveloped viruses are associated with cell membranes; the
type of cell membrane (plasma membrane,
nuclear membrane, endoplasmic reticulum, vacuoles) can
be a clue to virus identification.

SUMMARY OF VIRUS TYPES (based on morphology):
Naked viruses
All human naked viruses are icosahedral (spherical).
There are 3 size ranges from 20-35 nm, 45-55 nm, 70-90 nm. Examples:
Small: parvovirus, enterovirus, rhinovirus
Medium: polyomavirus, papillomavirus
Large: rotavirus/reovirus, adenovirus
Enveloped viruses
All have a lipoprotein membrane on the outside of the
nucleocapsid. Most derive this layer by budding through
cell membranes; this pliable layer makes the virion
pleomorphic. Poxviruses synthesize their shell de novo;
sometimes they pick up an extra covering by budding. They are
not pleomorphic, but different varieties may be more ovoid,
others, brick-shaped.
Most range in size from 40-400 nm. (Filoviruses are 80 nm by up
to 1400 nm.)
The envelope has proteins on the outside that may look like
spikes or fuzz (e.g., influenza virus, coronavirus).
Sometimes the spikes are too short to be distinguished by EM,
making the virion appear smooth (e.g., herpesviruses,
rubella virus).
The nucleocapsid shape can be
a. icosahedral, like the naked viruses; however, some
are morphologically nondescript as seen by EM;
b. filamentous, like a "slinky."
c. complex (poxvirus cores look like dumbbells with
lateral bodies)
d. morphologically nondescript (just dense without any
particular shape).
Enveloped DNA viruses (except pox) construct their nucleocapsids
in the nucleus.
Enveloped RNA viruses construct their nucleocapsids in the
cytoplasm; (measles nucleocapsids can sometimes be seen
in the nucleus).



On Sat, 26 Feb 2000, Ronald R. Matias wrote:

} Date: Sat, 26 Feb 2000 08:19:49 +0800
} From: Ronald R. Matias {rrm-at-skyinet.net}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need help on EM negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello and Help!
}
} We are a hospital based research lab. starting to use the electron
} microscope to identify or confirm pathogens that cause gastrointestinal
} disturbances. We would like to know if someone out there could recommend
} appropriate specific EM protocols (negative staining, processing of the
} gastrointestinal contents, etc....) to visualize these pathogens (ranging
} from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
} the routine EM preparations designed to examine tissues.
}
} In the interest of bring quality healthcare to our people, we will be very
} much obliged,
}
} ronnie matias
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Mon Feb 28 19:06:51 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 12:38:41 -0500 (EST)
Subject: Re: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ronnie,

We perform about 1000 exams/yr for viruses using EM. Below are some refs
(check out especially 2, 5, 9, 11, 14). Also, at the bottom is a
synopsis I give to our medical students; maybe it will be useful.

As for enteric viruses, EM is the best way to look for them. Many of
them do not grow in tissue culture, and those that can be coaxed, do so
under special conditions that are not employed in the routine culture
lab. Biochemical identification (e.g., immunological kits, PCR, Western
Blots) require a specific reagent to recognize the virus and if you
don't use the right reagent, you will miss viruses (e.g., if you run a
test for rotavirus, you will miss adenovirus, etc.) Furthermore, there
aren't biochemical reagents for all viruses. With EM, you can
see a wide variety of viruses by doing a negative stain of an aqueous
extract of stool.

Good luck,
Sara

REFERENCES ON VIRUS IDENTIFICATION BY ELECTRON MICROSCOPY

1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses
associated with HIV infection in Tanzanian children with chronic
diarrhea. Ped AIDS HIV Inf 5:296-299.

2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic
Virology: A Practical Guide and Atlas, Cambridge University Press, New York.

3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE,
Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro
LS, and Jaqua M-J. 1990. Six year retrospective surveillance of
gastroenteritis viruses identified at ten electron microscopy centers in
the United States and Canada Pediatr Infect Dis J 9:709-714.

4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus
Infections, CRC Press, Boca Raton, FL.

5. Miller SE. 1986. Detection and identification of viruses by
electron microscopy. J Electron Microsc Tech 4:265-301.

5. Miller SE, Howell DN. 1988. Viral infections in the acquired
immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.

7. Miller SE. 1988. Diagnostic virology by electron microscopy.
ASM News 54:475-481.

8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis.
EMSA Bull 19:53-59.

9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and
Methods. McGraw-Hill, New York.

10. Miller SE. 1991. Evaluation of electron microscopic information
available from clinical samples. In de la Maza LM, Peterson EM (eds.),
Medical Virology, Vol 10. Irvine, CA. pp 21-54.

11. Miller SE. 1995. Diagnosis of viral infection by electron
microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc, Washington, DC. pp 35-76.

12. Oshiro LS, Miller SE. 1992. Application of electron microscopy
to the diagnosis of viral infections. In Lennette EH (ed), Laboratory
Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.

13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC
Press, Boca Raton, FL.

14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral
Diagnosis, CRC Press, Boca Raton, FL.

15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP
Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New York.

2, 5, 9, 11-14 = Methods and micrographs
2, 14 = Excellent atlases
7, 8 = Brief synopses of technique and basis of virus ID
15 = Good general reference, but don't memorize!! Lists all viruses, not
just human.



TWO WAYS TO LOOK AT VIRUSES BY EM:
Negative staining
Used with liquid samples
Shows whole virus (size, surface structure important)
Thin sectioning
Used with cells and tissues
Shows a slice of an infected cell
Shows relationship of viruses to cell organelles (internal
structure and cell organelle association important)
Location within the cell is a clue to nucleic acid type
DNA viruses are usually seen in the nucleus (some
exceptions).
RNA viruses are usually seen in the cytoplasm
(some exceptions).
Exceptions:
Naked viruses get out of the cell by lysis; if
the cell is very sick, there may be virions
everywhere, e.g., adenovirus (DNA).
Poxviruses are DNA viruses, but are constructed
in the cytoplasm.
Hepatitis B core particles (DNA) may be in the
nucleus and cytoplasm. Dane particles
(complete viruses) are only cytoplasmic.
Some paramyxovirus (RNA) nucleocapsids, not the
complete virion, can be seen in the nucleus
(e.g., measlesvirus).
Enveloped viruses are associated with cell membranes; the
type of cell membrane (plasma membrane,
nuclear membrane, endoplasmic reticulum, vacuoles) can
be a clue to virus identification.

SUMMARY OF VIRUS TYPES (based on morphology):
Naked viruses
All human naked viruses are icosahedral (spherical).
There are 3 size ranges from 20-35 nm, 45-55 nm, 70-90 nm. Examples:
Small: parvovirus, enterovirus, rhinovirus
Medium: polyomavirus, papillomavirus
Large: rotavirus/reovirus, adenovirus
Enveloped viruses
All have a lipoprotein membrane on the outside of the
nucleocapsid. Most derive this layer by budding through
cell membranes; this pliable layer makes the virion
pleomorphic. Poxviruses synthesize their shell de novo;
sometimes they pick up an extra covering by budding. They are
not pleomorphic, but different varieties may be more ovoid,
others, brick-shaped.
Most range in size from 40-400 nm. (Filoviruses are 80 nm by up
to 1400 nm.)
The envelope has proteins on the outside that may look like
spikes or fuzz (e.g., influenza virus, coronavirus).
Sometimes the spikes are too short to be distinguished by EM,
making the virion appear smooth (e.g., herpesviruses,
rubella virus).
The nucleocapsid shape can be
a. icosahedral, like the naked viruses; however, some
are morphologically nondescript as seen by EM;
b. filamentous, like a "slinky."
c. complex (poxvirus cores look like dumbbells with
lateral bodies)
d. morphologically nondescript (just dense without any
particular shape).
Enveloped DNA viruses (except pox) construct their nucleocapsids
in the nucleus.
Enveloped RNA viruses construct their nucleocapsids in the
cytoplasm; (measles nucleocapsids can sometimes be seen
in the nucleus).



On Sat, 26 Feb 2000, Ronald R. Matias wrote:

} Date: Sat, 26 Feb 2000 08:19:49 +0800
} From: Ronald R. Matias {rrm-at-skyinet.net}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need help on EM negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello and Help!
}
} We are a hospital based research lab. starting to use the electron
} microscope to identify or confirm pathogens that cause gastrointestinal
} disturbances. We would like to know if someone out there could recommend
} appropriate specific EM protocols (negative staining, processing of the
} gastrointestinal contents, etc....) to visualize these pathogens (ranging
} from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
} the routine EM preparations designed to examine tissues.
}
} In the interest of bring quality healthcare to our people, we will be very
} much obliged,
}
} ronnie matias
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Mon Feb 28 19:06:52 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Mon, 28 Feb 2000 12:49:27 -0500 (EST)
Subject: Position Available (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------




---------- Forwarded message ----------





RESEARCH ASSOCIATE

Electron Microscopy Facility

University of Kentucky


The University of Kentucky invites applications for a Research Associate
in the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286



***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

Director, Electron Microscopy Facility

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/






From daemon Mon Feb 28 19:06:53 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Mon, 28 Feb 2000 12:49:27 -0500 (EST)
Subject: Position Available (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





RESEARCH ASSOCIATE

Electron Microscopy Facility

University of Kentucky


The University of Kentucky invites applications for a Research Associate
in the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286



***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

Director, Electron Microscopy Facility

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/






From daemon Mon Feb 28 19:06:54 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Mon, 28 Feb 2000 11:31:04 -0700
Subject: FW: Image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Supanee,

I don't know if you have received an answer since you posted this
message, but if you'd like, we could have a look at your images and try
to help you. What we need is of course the image(s) and a description of
what you actually want to measure. Please be as specific as you can.
Please send the material to my email address below.

Thanks.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Exchange Administrator
Sent: Wednesday, February 23, 2000 8:35 PM
To: Michael Bode


Dear all,
I am doing the image analysis of SEM micrographs. The
micrographs contain both dense (light) and air cell (dark) areas. But
two
areas are not discrete. I'm having a difficulty of quantifying those
separated fractions. I wonder if anyone has any ideas of how to
solve the problem.
Looking forward for your suggestions.
Regards,
Supanee



From daemon Mon Feb 28 19:06:55 2000



From: George Lawton :      George.Lawton-at-email.swmed.edu
Date: Mon, 28 Feb 2000 12:48:57 -0600
Subject: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Occassionally someone sends a question to the listserver and there is no response. It doesn't happen often so I thought I would send this question again.


Several weeks ago, I was given some inner membranes from E. coli to be run up to Epon and sectioned for TEM. I treated the sample like tissue culture and pelleted the membranes. Fixed in
3% glut in PBS and post-fixed in 1% OsO4. Routine dehydration in alcohols. Embedded in
EMbed 812 with BDMA in Beem Capsules. Left in oven 48 hours. When I tried to section, the tissue was too soft and appeared to not be infiltrated well. Put back into the oven for 2-3 more days. No difference. Thinking I had a neural meltdown while processing the samples, I tried again on fresh samples, paying particular attention to details. I also used new bottles of embedding media. Same results.
The part of the block without the tissue is its usual hardness. The part of the block with the tissue
(the tip of the beem capsule) is soft and tacky.

Has anyone experienced anything like this? Any suggestions on how I can get the sample into epon?

Thanks in advance for any ideas and/or suggestions.

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu



From daemon Mon Feb 28 19:06:59 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 28 Feb 2000 15:08:21 -0500
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you were working with a pellet in the bottom of a tube or BEEm cap, it
is possible that you were not getting good exchange of reagents at the tip
of the tube during dehydration and embedding.
Or is you were centifuging in the final resin mixture before
putting into the oven it is possible that your sample was packed so tightly
that there was not enough resin in it to polymerize properly.


At 12:48 PM 02/28/2000 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Mon Feb 28 19:07:01 2000



From: Margie Bryant :      mbryant-at-com1.med.usf.edu
Date: Feb.28, 2000
Subject: Leitz Ultrapak incident-Light illuminator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

We have an old Leitz Orthoplan microscope and are
looking for the Ultrapak incident light illuminator (housing with fixed
ring mirror and interchangeable UO objectives of 4X to 60X primary
magnification). These components have not been produced in many years.
If anyone has this equipment, we would be pleased to pay for it and for
the shipping. Thank you very much!

Sincerely,

Margie Bryant

From: Margie
Bryant {mbryant-at-com1.med.usf.edu



From daemon Mon Feb 28 19:07:01 2000



From: przybylo :      przybylowicz-at-nac.ac.za
Date: Mon, 28 Feb 2000 23:08:46 +0200
Subject: As in biological tissues

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Dear Doug,

We have some experience in using micro-PIXE (proton induced X-ray emission)
and the so-called nuclear microprobe in toxicological research. It is more
sensitive than EDS and WDS, is very easy to quantify (standardless
technique). In principle you can have detection limits of the order of 1
ppm in point analysis mode. Specimens can be of any thickness and their
actual thickness is assessed by the simultaneous use of the BS technique
(proton backscatering spectrometry). What in my opinion makes it really
attractive for this purpose is its very good scanning capability. Scan sizes
are from ca. 2.5 mm x 2.5 mm down to the beam resolution, which is of the
order of few micrometer (can be down to 1 micrometer or even less, but in
most of our uses few micrometers is good enough).
The sample preparation is similar as for EDS, using cryotechniques to avoid
elemental losses and redistribution.
We have so far done studies on Ni, Mo, Cd. The detection limits for As are
better than for Mo and Cd.
Of course, this technique cannot indicate the state of oxidation, you can
really treat it as a "more sensitive EDS".

If you want more information, I can refer you to our earlier work and
perhaps you might be interested in contacting me.

Best regards


xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: przybylowicz-at-nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820
http://www.nac.ac.za/IBA/
http://www.nac.ac.za/materials/
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Mon Feb 28 19:07:02 2000



From: rlvaughn-at-unmc.edu
Date: Mon, 28 Feb 2000 15:15:58 -0600
Subject: network printing management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I seem to recall a discussion of software that was available to keep track
of printing jobs submitted to a network printer for billing purposes? An
argument here for not installing a networked dyesub printer for digital
images is who is going to be responsible for watching who's using it and
how many prints they're making. We have had some problems like this with
the Epson 850 which is on the network and somebody has ran through two
color cartridges in a short time. Thanks for any advice.

Rick Vaughn
rlvaughn-at-unmc.edu



From daemon Mon Feb 28 19:07:07 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 28 Feb 2000 17:27:59 -0600
Subject: Image Analysis of Spheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a program (preferably Macintosh-based) that will
automatically measure the diameter of spheres that have been imaged
in a TEM.

Typically, the spheres measure about 3-5 mm on the TEM negative and
often are touching or slightly overlapped. I know that NIH Image
should do this, but I have not been able to get reproduceable data
using this program.

Someone told me about a program called "Bolero", written by some
Spanish university researchers but it is an $8-10K program. Too rich
for our tastes.

Any suggestions?

We do appreciate your suggestions.

John

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Mon Feb 28 19:07:09 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Mon, 28 Feb 2000 16:36:18 -0700
Subject: bio: peptidoglycan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a way to label for peptidoglycan at the light or
electron microscope level?

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920



From daemon Mon Feb 28 19:07:09 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 18:42:51 -0500 (EST)
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Greg: sounds like an infiltration problem. Your best bet
is to encase the bugs in agar. One way is to fix briefly (2-5 min) in glut,
then pellet and let sit for a while (1 hr). This glues them together.

Don't resuspend them in the glut or it will fix the walls of the bugs so
that they won't stick together.

Cut off the bottom of the
tube if plastic or scrape out the cells if tube is glass, onto Parafilm
and drain with wedge of filter paper. Divide into 1 cubic mm (or
smaller) portions and cover with molter agar. Cut away any excess.
Wash. Osmicate and embed as you would a piece of tissue.

Other folks have suspended bugs in molter agar and pelleted them through
the agar. You have to do this before the agar cools. Then cut off the
end with the cells.

Don't fix the agar/cells in glut; it will cross-link the agar so that the
subsequent solutions can't get in. The glut left in the bugs after
they're fixed and before they're encased is OK and will wash out.

Hope this helps.

Sara


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Mon Feb 28 19:07:09 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 28 Feb 2000 18:49:03 EST
Subject: Re: Image Analysis of Spheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 2/28/00 11:44:37 PM, bozzola-at-siu.edu writes:

} Typically, the spheres measure about 3-5 mm on the TEM negative and
} often are touching or slightly overlapped. I know that NIH Image
} should do this, but I have not been able to get reproduceable data
} using this program.

If none of the spheres have been cut off in the section so that you have
stereological corrections to make for the missing parts, and you aren't
having problems with thresholding, etc., then I don't see what your problem
is. Use a watershed to separate the touching features, and use the maximum
feret's diameter to estimate the sphere diameter, not the area (which may be
reduced due to overaps). Or you can use the maximum values of the euclidean
distance map (the ultimate eroded point) as the sphere radii and not even
bother with the watershed.



From daemon Mon Feb 28 21:37:56 2000



From: rachel.harding-at-oxinst.co.uk
Date: Mon, 28 Feb 2000 19:10:48 -0600
Subject: info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Is there a web site where I can find more information about quantitative
techniques in microscopy on SEM's?
In particular the XPP correction procedure?

Many thanks
Rachel


____________________________________________________________
Oxford Instruments Analytical, Halifax Road, High Wycombe, Bucks,
HP12 3SE, UK Tel: +44 (0)1494 442255 Fax: +44 (0)1494 524129
http://www.oxford-instruments.com/ We have a 5M email size limit

Unless stated above to be non-confidential, this E-mail and any
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If you have received this E-mail in error please notify us upon receipt
and delete it from your records. Internet communications are not secure
and Oxford Instruments is not responsible for their abuse by third
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____________________________________________________________




From daemon Mon Feb 28 21:38:03 2000



From: mtl :      mtl-at-njcc.com
Date: Fri, 01 Jan 1999 21:33:41 -0400
Subject: Re: Leitz Ultrapak incident-Light illuminator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may want to try Triad Scientific Inc. in Edison, NJ. ,
triadsci-at-webspan.net or (800) 867-6690. When I was there last month to
purchase a vacuum oven, a whole truck lot of optical microscopes and parts
(Leitz, etc.) was being unloaded. Most were still in the original boxes.
J. Roy Nelson
Material Testing Laboratory
Pennington, NJ
mtl-at-njcc.com

Margie Bryant wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Date: Feb.28, 2000
}
} Hello,
}
} We have an old Leitz Orthoplan microscope and are
} looking for the Ultrapak incident light illuminator (housing with fixed
} ring mirror and interchangeable UO objectives of 4X to 60X primary
} magnification). These components have not been produced in many years.
} If anyone has this equipment, we would be pleased to pay for it and for
} the shipping. Thank you very much!
}
} Sincerely,
}
} Margie Bryant
}
} From: Margie
} Bryant {mbryant-at-com1.med.usf.edu



From daemon Mon Feb 28 21:38:05 2000



From: Marilyn Henderson :      marilyn.henderson-at-adelaide.edu.au
Date: Tue, 29 Feb 2000 13:57:34 +1030
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear George,

I've found the same problem sometimes with Epon/Araldite in Eppendorf
tubes. I think the pellet is so tightly bound to the bottom of the tube
by the time 100% resin infiltration is reached (especially if you
centrifuge along the way) that the resin doesn't have good access to the
cells or material at the 'bottom'. I now lift (ease gently) the pellet
off the bottom of the tube with a toothpick to ensure that the media are
totally surrounding the pellet.
Another choice is to cut away all the gooey resin and hope that the
material at the 'top' of the pellet is well infiltrated and polymerized.

Regards,
Marilyn

George Lawton wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Occassionally someone sends a question to the listserver and there is no response. It doesn't happen often so I thought I would send this question again.
}
} Several weeks ago, I was given some inner membranes from E. coli to be run up to Epon and sectioned for TEM. I treated the sample like tissue culture and pelleted the membranes. Fixed in
} 3% glut in PBS and post-fixed in 1% OsO4. Routine dehydration in alcohols. Embedded in
} EMbed 812 with BDMA in Beem Capsules. Left in oven 48 hours. When I tried to section, the tissue was too soft and appeared to not be infiltrated well. Put back into the oven for 2-3 more days. No difference. Thinking I had a neural meltdown while processing the samples, I tried again on fresh samples, paying particular attention to details. I also used new bottles of embedding media. Same results.
} The part of the block without the tissue is its usual hardness. The part of the block with the tissue
} (the tip of the beem capsule) is soft and tacky.
}
} Has anyone experienced anything like this? Any suggestions on how I can get the sample into epon?
}
} Thanks in advance for any ideas and/or suggestions.
}
} George Lawton
} Chief Electron Microscopist
} Molecular and Cellular Imaging Facility
} UT Southwestern Medical Center at Dallas
} 5323 Harry Hines Blvd.
} Dallas, Tx 75390-9039
} Phone: 214-648-7291
} eMail: George.Lawton-at-email.swmed.edu



From daemon Tue Feb 29 07:09:16 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 28 Feb 2000 18:37:42 -1000 (HST)
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi-

Two things come to mind. First, if processed in a microfuge tube, loosen
the pellet from the tube and make sure all fluids and epoxy mixtures come
into contact with all surfaces (as already recommended). Second, I always
make it a practice to preheat the BEEM capsules and labels in the
embedding oven all day or overnight before use to drive off any
moisture. This really seems to help in our humid environment!

Good luck and aloha,
Tina

Sunny and 80F but with mosquitoes.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Tue Feb 29 07:39:41 2000



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Tue, 29 Feb 2000 08:20:26 -0500
Subject: Re: network printing management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick:
What you want to do is fairly easy if you are using Windows NT. It will
maintain a log of the user name, number of prints, date, name of print job,
etc. I posted instructions to this group in 1997. These instructions were
published in Microscopy and Microanalysis Vol.3, number 6, p. 569. If you
don't have access to this, let me know and I will send the directions.
Stanley L. Flegler, Acting Director
Center for Advanced Microscopy
Michigan State University



From daemon Tue Feb 29 07:39:42 2000



From: mccu5iv2-at-fs2.ee.umist.ac.uk ()
Date: Tue, 29 Feb 2000 07:19:44 -0600
Subject: HREM of semiconductors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: mccu5iv2-at-fs2.ee.umist.ac.uk
Name: Imran

School: Vahora

Question: Currently studying for a project on HREM of semiconductors,
namely GaN; I would like to know where I can obtain some good information
on this subject. My lecturer does not have much insight in this area, I
need to study its structure, and I am currently using the Cerius(2)
software package to simulate images of GaN.

thankyou














---------------------------------------------------------------------------




From daemon Tue Feb 29 07:39:42 2000



From: Duncan Waddell :      D.Waddell-at-mailbox.uq.edu.au
Date: Tue, 29 Feb 2000 07:31:01 -0600
Subject: CyberSTEM demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Centre for Microscopy & Microanalysis (CMM) at The University of
Queensland will demonstrate its web-based interactive Virtual Electron
Microscopy service this Thursday (March 2, 2000) between 11am and 1pm.
(Queensland Time; GMT +10)

CyberSTEM (WWW accessible Scanning and Transmission Electron Microscopy)
is a service that became officially permanent on the web after Centre
Directors approved funding last week.

URL: http://www.uq.edu.au/nanoworld/online.html

The CMM's videoconference service - begun in 1995 - has evolved into a
webcam-based service that allows unlimited live connections to
microscopy sessions conducted at the Centre. Visitors can interact with
staff during organised sessions via telephone, by chat programs or via
email. (Please note: Live interaction is only available during
specifically organised sessions - direct all other inquiries to the
address/email noted in the signature below.)

Though primarily intended to service paying customers who cannot
physically attend sessions, video streaming from two of their
microscopes (more later they hope) will be regularly accessible by the
general public.

Sincerely,

Duncan

--
************************************************************
Duncan Waddell (BSc)
Senior Scientific Officer
Centre for Microscopy and Microanalysis
The University of Queensland, St. Lucia, Qld, 4072 Australia
Telephone: +61-7-3365-4216
Facsimile: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/nanohome.html
************************************************************
Any opinion expressed is that of the writer,
and not necessarily that of CMM or of the University.
************************************************************




From daemon Tue Feb 29 07:49:37 2000



From: Maria.Fazio-Zanakis-at-aventis.com
Date: Tue, 29 Feb 2000 07:37:17 -0600
Subject: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear George,
In all my experience working up bacteria and bacterial fragments, I have
always, and by the way this is the simplest, filtered all suspended cells on
Millipore filters and processed the samples on the filters. This yielded
excellent results. The fragments, cells stayed on the filters and never
detached.
Filter your suspended fragment solution using a 0.2um filter unit system
attached to a vacuum system. The aspirator of a faucet works great. Rinse
in buffer while on the filter system.
Then remove the filter and gently place in a plastic or glass petri dish.
Again, gently drop fixative over the sample enough to cover your filter and
carefully slice it into your appropriate sizes and process from there.
You will have success. If you have further questions, just give me a call.

Blessings,
Maria

Maria Fazio-Zanakis
Bioimaging and Molecular Histology
Aventis Pharmaceuticals
1-908-231-3357
Fax: 1-908-231-3962
Email: Maria.Fazio-Zanakis-at-Aventis.com


-----Original Message-----
} From: George Lawton [mailto:George.Lawton-at-email.swmed.edu]
Sent: Monday, February 28, 2000 1:49 PM
To: microscopy-at-sparc5.microscopy.com


Occassionally someone sends a question to the listserver and there is no
response. It doesn't happen often so I thought I would send this question
again.


Several weeks ago, I was given some inner membranes from E. coli to be run
up to Epon and sectioned for TEM. I treated the sample like tissue culture
and pelleted the membranes. Fixed in
3% glut in PBS and post-fixed in 1% OsO4. Routine dehydration in alcohols.
Embedded in
EMbed 812 with BDMA in Beem Capsules. Left in oven 48 hours. When I tried
to section, the tissue was too soft and appeared to not be infiltrated well.
Put back into the oven for 2-3 more days. No difference. Thinking I had a
neural meltdown while processing the samples, I tried again on fresh
samples, paying particular attention to details. I also used new bottles of
embedding media. Same results.
The part of the block without the tissue is its usual hardness. The part of
the block with the tissue
(the tip of the beem capsule) is soft and tacky.

Has anyone experienced anything like this? Any suggestions on how I can get
the sample into epon?

Thanks in advance for any ideas and/or suggestions.

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu



From daemon Tue Feb 29 18:24:15 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 29 Feb 2000 06:23:04 -0800
Subject: S-520

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have a customer who wants to purchase a Hitachi S-520 (or equivalent)
SEM with EDS.

If anyone has anything, please contact me off line.

Thank You,

Earl Weltmer
(714) 573-9158





From daemon Tue Feb 29 18:24:15 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 29 Feb 2000 06:23:18 -0800
Subject: S-520

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have a customer who wants to purchase a Hitachi S-520 (or equivalent)
SEM with EDS.

If anyone has anything, please contact me off line.

Thank You,

Earl Weltmer
(714) 573-9158





From daemon Tue Feb 29 18:24:19 2000



From: Fiona Russell :      fiona.russell-at-gmx.net
Date: Tue, 29 Feb 2000 16:23:06 +0100 (MET)
Subject: Help on: Plant Cell Chromosome Counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone help me?
I am doing research on the plant Achillea millefolium (Yarrow)and I need
to find out the ploidy level of 2 seed sources. I am looking for information
on companies/institutions that will do this work for me and a rough estimate
of the cost.
Many thanks,
Fiona Russell.

--
Sent through Global Message Exchange - http://www.gmx.net



From daemon Tue Feb 29 18:24:29 2000



From: Steve Miller :      smiller-at-ventanamed.com
Date: Tue, 29 Feb 2000 13:37:31 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Tue Feb 29 18:24:31 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 29 Feb 2000 15:20:37 -0700 (MST)
Subject: Oversimplification-Sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I recently tried to help out Dr. M. Rohde who was embedding in Spurr's
formulation and attempting to locate an antigen.

I said that Spurr's formulation has no place in immunocytochemistry for
all the reasons I listed then. This is an oversimplification (I am always
harassed for time when trying to answer questions completely.) Let me
tell you that I, myself, worked with a graduate student who used Spurr's
medium, etched for one hour in sodium metaperiodate, and then got
wonderful Au label. The reason for this success was that she had an
antigen that was so sturdy and such plentiful supply that it was a
candidate for the prozone effect. Her same exact protocol (actually mine)
when tried by us in our laboratory was a spectacular failure with our
antigen which we soon discovered was scarce and delicate and needed
amplification up to the point where I wondered whether we were actually
increasing the thickness of the section by amplification methods! Sort of
like painting a wall until the room gets smaller!
At any rate - yes, Spurr's can be used for immunocytochemistry. However,
if at first you don't succeed, don't try again. Go to another epoxy, and
then to an acrylic.
I should not make absolute statements as I did. I did not mean to
discourage those who successfully use Spurr's to abandon it. Besides,
compared to what there is to be known about immunocytochemistry, my amount
of knowledge can be dropped into a thimble with room left to stuff an
entire thumb in after it.

I always reserve the right to be wrong!

Hildy Crowley
Sr. Electron Microscopy Specialist
Dep. of Biol. Sciences
University of Denver
Denver, CO



From daemon Tue Feb 29 18:24:31 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Tue, 29 Feb 2000 16:38:26 -0600
Subject: General: Web sign-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I remember seeing posts over the years regarding web-based equipment
sign-up systems, but didn't save any of them. Can anyone direct me to
an archived discussion thread, or have current suggestions on a very
simple and easy way to set this up? I want to put this under a dept.
web page on our intranet only, so security is not a major issue. All I
want is a simple calendar that users can view and sign up for time
slots. No retribution if time not used, but must require user's name so
that they can be tracked down for questioning if needed. I have no
experience in HTML programming but am not afraid to learn.

Thanks as always,
Karen

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Tue Feb 29 18:24:33 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Tue, 29 Feb 2000 16:45:18 -0600
Subject: General: Video Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any suggestions on a really good video printer to include with an SEM
purchase? We've had trouble over the years with the standard roll
printers getting paper jams, which required sending out for servicing
(i.e. lots of down time). We're willing to sacrifice some speed for
something reliable. Are there "fast" ( { 1 min. per print) video
printers that use the cut sheet paper?

Thanks,
Karen

P.S. vendors please respond by E-mail (not phone) or dare to face my
wrath.
--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Tue Feb 29 18:24:36 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Tue, 29 Feb 2000 15:09:31 -0800
Subject: subcribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




From daemon Tue Feb 29 18:24:39 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 29 Feb 2000 17:11:10 -0700
Subject: Tracor Northern 5500 EDX Units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have 2 Tracor Northern/Noran TN 5500 EDX systems that need a new home. We
are looking for a possible trade for comparable priced equipment/software.
If anyone is interested, please contact me directly.

Harry Ekstrom
Honeywell Materials Laboratory
Phoenix, AZ
602-231-2744



From daemon Tue Feb 29 23:00:41 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 29 Feb 2000 19:59:14 -0500
Subject: TEM: Tripod Polisherš Workshop - Date

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry for the inconvenience, but we mistakenly scheduled our Tripod
Polisherš Workshop over Memorial Day Weekend. While I'm sure that you
would all love to spend your holiday weekend here with us, we felt it
necessary to move the workshop to the following week. The workshop is now
scheduled for June 2- 3, 2000 in San Clemente, CA.

For those of you who have already registered, you should have already been
contacted and given the option of re-booking for the new date or canceling
your reservation. Again, I apologize for the error. Certainly, if anyone
wishes to come and visit us over the Memorial Day Weekend, we will welcome
you with open arms - but you'll have to wait until the following weekend
for the workshop!

Best regards-

David
Writing at 5:12:19 PM on 02/29/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.





From daemon Wed Mar 01 07:49:36 2000



From: Evex :      evex-at-evex.com
Date: Wed, 1 Mar 2000 06:43:21 -0500
Subject: S-520

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl,

Evex Analytical ( www.evex.com ) has a Jeol 840 with an Evex =3D
Microanalysis system ( http://www.evex.com/prod2.htm ) and Active Digital
Imaging System that we would like to replace with a new microscope.

Claudio Tarquinio
Evex Analytical
Microanalysis and Digital Imaging
609-252-9192 T
609-252-9091 F
www.evex.com
info-at-evex.com





From daemon Wed Mar 01 07:49:37 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 1 Mar 2000 08:44:30 -0400
Subject: Re: General: Video Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




----------
} From: Karen Zaruba {kszaruba-at-MMM.COM}
} To: MSA Listserve {microscopy-at-sparc5.microscopy.com}
} Subject: General: Video Printers
} Date: February 29, 2000 6:45 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Any suggestions on a really good video printer to include with an SEM
} purchase? We've had trouble over the years with the standard roll
} printers getting paper jams, which required sending out for servicing
} (i.e. lots of down time). We're willing to sacrifice some speed for
} something reliable. Are there "fast" ( { 1 min. per print) video
} printers that use the cut sheet paper?
}
} Thanks,
} Karen
}
} P.S. vendors please respond by E-mail (not phone) or dare to face my
} wrath.
} --
} Karen S. Zaruba kszaruba-at-mmm.com
} 3M Company, St. Paul, MN
}

Karen -

We've always been happy with our little Sony UP-811 video printer. It
doesn't give a very big print (about 5 inches wide), rather like a
Polaroid, and not particularly high resolution or anything, but useful as a
"working copy", with the actual image saved to disk. It's dead reliable,
however, having been in continuous service since about 1993, with no
problems that I know of.
No connection with Sony, etc. (wouldn't mind owning some stock, though;-)

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada
Dartmouth, Nova Scotia



From daemon Wed Mar 01 07:49:39 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Wed, 1 Mar 2000 07:57:00 -0600
Subject: Re:General: Video Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Karen,

I had a Mitsubishi that was reliable, but in general, my feeling is that for
reproducing EM images there are NO good video printers. Between the
(typical)
paper cost and poor resolution, I prefer to print captured digital images.
Though slow, you can get an inkjet printer these days for 1/10 the price.
Faster ones for a bit more...

Woody White



From daemon Wed Mar 01 08:08:39 2000



From: DARRYL GRAY :      ddg99-at-aber.ac.uk
Date: Wed, 1 Mar 2000 07:52:35 -0600
Subject: LTSEM : Looking for Literature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues.... Can anyone help this guy? It's not my area.

Nestor
Your Friendly Neighborhood SysOp
------------------------------------------

Dear Dr Zaluzec

I'd be very grateful if you could help me with a problem I'm having.

I'm writing an essay in low temperature scanning electron microscopy in
biology, but I'm finding it difficult to track down up-to-date source
material.

If you could suggest any papers, books or book chapters related to the
subject, it would be a great help. Thanks.

Darryl Gray




From daemon Wed Mar 01 17:51:12 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 1 Mar 2000 08:24:54 -0600
Subject: LTSEM : Looking for Literature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Darryl,

The following web site, http://www.emitech.demon.co.uk/, has a downloadable
technical brief on low temperature EM. It talks about Emitech's products,
but also has a discussion of low-temp EM in more general terms. A more
dated, but very useful, reference is the book "Low Temperature Methods in
Biological Electron Microscopy", which is part of the "Practical Methods in
Electron Microscopy" series edited by Audrey Glauert. My copy is dated
1985. I don't know if later editions exist.

Hope this is useful.

Disclaimer: We have no financial interest in Emitech's products, other than
being users of them.

Regards,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: DARRYL GRAY [mailto:ddg99-at-aber.ac.uk]
Sent: Wednesday, March 01, 2000 7:53 AM
To: Microscopy-at-sparc5.microscopy.com


Colleagues.... Can anyone help this guy? It's not my area.

Nestor
Your Friendly Neighborhood SysOp
------------------------------------------

Dear Dr Zaluzec

I'd be very grateful if you could help me with a problem I'm having.

I'm writing an essay in low temperature scanning electron microscopy in
biology, but I'm finding it difficult to track down up-to-date source
material.

If you could suggest any papers, books or book chapters related to the
subject, it would be a great help. Thanks.

Darryl Gray




From daemon Wed Mar 01 17:51:17 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Wed, 01 Mar 2000 12:04:57 -0600
Subject: Re: Proteoglycan staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry I deleted the original question so I don't remember who posted
it. Then I came across a couple of papers by John E. Scott on staining
for PG's using Cupromeronic Blue. Never tried this myself, so I don't
know how well it works. Here's one reference:

J.E. Scott and M. Haigh, "Identification of specific binding sites for
keratan sulphate proteoglycans and chondroitin-dermatan sulphate
proteoglycans on collagen fibrils in cornea by the use of Cupromeronic
Blue in 'critical-electrolyte-concentration' techniques." Biochem J.
(1988) 253:607-610.

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Wed Mar 01 17:51:17 2000



From: Ghanem, Antoine :      Antoine.Ghanem-at-solvay.com
Date: Wed, 1 Mar 2000 18:56:48 +0100
Subject: TEM - Staining of PCL and PVDF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear microscopists,

Does anybody know a recipe for staining the amorphous part of
poly(vinylidene fluoride) and poly (e-caprolactone)? We need to view the
crystalline lamellae of these polymers by TEM.

Thank you in advance for your help.

Antoine

======================
Dr Antoine GHANEM
SOLVAY Research and Technology
Rue de Ransbeek 310
1120 Brussels
Belgium
Phone 32-2-2643422
Fax 32-2-2642055
antoine.ghanem-at-solvay.com
======================



From daemon Wed Mar 01 17:51:18 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 1 Mar 2000 10:57:57 -0800
Subject: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Someone in the lab needs help with hand sectioning Arabidopsis seedling
stems. They are small, less than a mm in dia. and very delicate. She needs
cross sections of the stem from a specific zone to do microscopy and
pigment localization.

I tried it using traditional techniques, but without success. She must have
sections of fresh material for her stains, she says frozen sections don't
work. Any ideas?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Mar 01 17:51:18 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 01 Mar 2000 14:41:06 -0500
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Suspend them in some stiff agarose and try a vibratome. There is also the
old elder pith trick, if you haven't already tried it. There are only a
few of us ancient enough to have ever heard of that one.


At 10:57 AM 03/01/2000 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Wed Mar 01 17:51:19 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 1 Mar 2000 13:47:11 -0600
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
Jonathan Krupp asked:

}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
We have made some headway in a related problem by encasing
the fresh tissue in low melting point agarose, around 3%. The idea
here is to make a cube of agarose with the stem oriented in side. You
can then cut the whole cube with a razor. The agarose doesn't impede
or interfere with most stains. This didn't work perfectly, but it was
better than nothing.

Hope this helps,
Tobias Baskin
_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed Mar 01 17:51:19 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 1 Mar 2000 16:21:24 -0400
Subject: Re: Proteoglycan staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are the most recent posts. I plan to use the calendar from brownbear
but have not actually implemented it yet.

http://www.brownbearsw.com

Date: Thu, 26 Aug 1999 14:45:39 -0400
Reply-To: Confocal Microscopy List {CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU}
Sender: Confocal Microscopy List {CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU}
} From: Kristi DeCourcy {decourcy-at-MAIL.VT.EDU}


At 12:04 PM -0600 3/1/0, Karen Zaruba wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

************************
You can also try any number of cationic stains (ruthenium red, alcian blue.
safranin-O, etc)at concentration of about 0.1-0.5%in the primary fixation
step. These dyes are electron dense and will bind to the negatively
charged PG's. You can also track down references from the 1970's & early
1980's by Simeonescu & Simeonescu who did a lot of work with these types of
dyes.
I have used them (years ago) to look at the extracellular matrix in heart
muscle.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed Mar 01 17:51:25 2000



From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Wed, 01 Mar 2000 17:12:49 -0500
Subject: TEM polymer help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a set of samples giving me fits here. This person is trying to
visualize the ruthenim doped into a polymer. I have not had much luck
working with this stuff and was wondering if any of you had any suggestions
to pass along. A description of the sample follows.Here is what I have
tried so far.

She originally brought this in on a glass slide and it is about 100µm
thick. I tried to use backscattering to visualize the Ru metal in the
FESEM. She thought it might be in little islands. No luck.
It was too thick to shoot a beam through with the TEM so I embedded some in
cold LR-White resin and sectioned. It offered enough support to get some
areas thin enough to view through, but I did not see any metal. I suggested
she take the grids to the Materials EM unit here on campus as the have EDS
and are better equipped for this, but they saw nothing either.

I then had her try to spin coat slids,grids,and mica to float off the film,
but she cannot get it to work so now I am back to the slides, scraping
material off, and cold embedding. The last set was too rubbery though and
even in epoxy on a good diamond I was unable to obtain a section of the
material. It just moved out of the way and popped back up with nice resin
sections floating away. I don't get any polymers here and we are almost
exclusively a biologic unit so do any of you have any suggestions??


} Date: Wed, 1 Mar 2000 11:28:21 -0500 (EST)
} From: Joanne Bedlek {bedlek-at-chem.ufl.edu}
} To: Scott Whittaker {sdw-at-biotech.ufl.edu}
} Subject: Re: your mail
}
} Scott,
}
} The polymer is a polydimethylsiloxane. The metal (Ru) is dissolved in
} methylene chloride and added to the silicone fluid. As the polymer cures
} it "hardens" to a rubbery material.
}
} The TEM images you took give us an idea of the polymer distribution. With
} the new films I have, we are interested in the polymer distribution again.
} I
} suspect we will see the same droplet pattern as before. This will be a
} characterization technique for a paper submission.
}
} As for the dispersion technique, MAIC tried EDS. They were not able to
} detect the Ru. They did tell me that there is a higher content of Si on
} the outer sides of the droplets as opposed to the interior areas. This is
} interesting.
}
} I will drop off the new samples either next week or the week after. It
} depends when I get back next Friday.
}
} I redid the one sample that was giving you so much trouble in slicing. I
} hope it has cured better this time.
}
}
} } This may be more of a dispersion thing. Materials should be able to see it
} } if it is in there. You might get with them and see if they have done
} } anything similar as we had no luck with that last set you brought in. I
} } could also post a discussion to the microscopy listserver and see what
} } comes up. I just need to know more about the composition of the samples
} and
} } stuff like that
} }





} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
EM Technician Gainesville, FL 32610
University Of Florida ph 352-392-1184
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "







From daemon Wed Mar 01 17:51:27 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 1 Mar 2000 18:01:23 -0500 (EST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with using the agar. Try 3% and go up if you need to. If the
stem is soft, you'll be able to cut it with a "Vibratome" or vibrating
microtome. If it is hard, you won't.

Sara

On Wed, 1 Mar 2000, Jon Krupp wrote:

} Date: Wed, 1 Mar 2000 10:57:57 -0800
} From: Jon Krupp {jmkrupp-at-cats.ucsc.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM, help with hand sectioning
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Wed Mar 01 18:40:04 2000



From: pfieber-at-elite.net
Date: Wed, 1 Mar 2000 18:33:46 -0600
Subject: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Email: pfieber-at-elite.net
Name: Paul Fieber

Question: I read a lot of information about Naturpaths using Dark Field
Microscopy for live blood analysis and blood crystallization testing. They
claim they can see nutritional deficiencies as well as disease process
through this type of testing. Is this possible and how accurate are these
tests? Thanks.

---------------------------------------------------------------------------




From daemon Wed Mar 01 18:40:04 2000



From: Richard G. Heller :      dickh-at-alb.edu
Date: Wed, 1 Mar 2000 18:35:10 -0600
Subject: Zeiss 960 SEM

Contents Retrieved from Microscopy Listserver Archives
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We have the opportunity to purchase a used Zeiss 960 SEM for use by
undergraduate biology students in a number of courses and for research.

I would be interested in any comments, advice, etc. about the instrument
and its appropriateness for this type of application, such as
maintenance, resistance to abuse, and ease of use.

Thank you

Dick Heller
Biology
Albright College

dickh-at-alb.edu




From daemon Thu Mar 02 01:08:09 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 1 Mar 2000 18:42:02 -0600
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Elder pith being in short supply you might try supporting it
in a fresh carrot. I have had some luck with that in a hand
microtome.

I came across the method in a book from the 30's.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
} From: "Greg Erdos" {gwe-at-biotech.ufl.edu}
}
}
} Suspend them in some stiff agarose and try a vibratome. There is also the
} old elder pith trick, if you haven't already tried it. There are only a
} few of us ancient enough to have ever heard of that one.
} }
} } Someone in the lab needs help with hand sectioning Arabidopsis seedling
} } stems. They are small, less than a mm in dia. and very delicate. She
needs
} } cross sections of the stem from a specific zone to do microscopy and
} } pigment localization.
} }
} } I tried it using traditional techniques, but without success. She must
have
} } sections of fresh material for her stains, she says frozen sections don't
} } work. Any ideas?
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
} }
} }
}
} Gregory W. Erdos, Ph.D. Ph.
352-392-1295
} Assistant Director, Biotechnology Program
} PO Box 110580
Fax:
} 352-846-0251
} University of Florida
} Gainesville, FL 32611
}
}
}




From daemon Thu Mar 02 01:08:17 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 2 Mar 2000 09:32:07 +1000
Subject: RE: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon, it seems that project is beyond hand sectioning. There is a whole series
of instruments available that use a oscillating or vibrating knife and the best
can section down to 10 micrometers. Buy, beg, borrow don't steal one of those
instruments. The mid to lower price range should do your job.
Disclaimer: ProSciTech sells these instruments
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 02, 2000 4:58 AM, Jon Krupp [SMTP:jmkrupp-at-cats.ucsc.edu]
wrote:
}
} Greetings:
}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}



From daemon Thu Mar 02 01:08:19 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Thu, 02 Mar 2000 15:54:30 +1100
Subject: Naturopaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Question: I read a lot of information about Naturpaths using Dark Field
} Microscopy for live blood analysis and blood crystallization testing. They
} claim they can see nutritional deficiencies as well as disease process
} through this type of testing. Is this possible and how accurate are these
} tests? Thanks.
}
} ---------------------------------------------------------------------------
}



You CAN see red cells. But the Naturopaths are only using this as a hook
to give (pseudo)scientific validity to their diagnosis. It all COMPLETE
HOKUM.
}
}
}



From daemon Thu Mar 02 08:09:35 2000



From: Drouillon, Philippe :      Philippe.Drouillon-at-solvay.com
Date: Thu, 2 Mar 2000 08:57:13 +0100
Subject: Help needed : a question of death or life...for our TEM microscop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

We are looking for a high voltage measurement cable (cable which links the
high voltage transformer to the measurement resistances) in working order
and dedicated to a Siemens Elmiskop 102 TEM (manufacturing model number
2810)

Here are the specifications of the cable we are looking for : 125 kV - 1.5 m
length

Would it be possible to also give us a cost estimation ?

Thanks in advance

Best regards

\\ _//
X-FERT ! -(-at- -at-)-
-------------------oOO--(_)--Ooo------------
Philippe Drouillon
Solvay Research and Technology
Electron Microscopy and Image Analysis - IT Coordinator
Rue de Ransbeek, 310
B-1120 Brussels (Belgium)
phone : (00 32) 2 264 24 47
mailto:philippe.drouillon-at-solvay.com

--------------------------Oooo.-------------
.oooO ( )
( ) ) /
\ ( (_/
\_)



From daemon Thu Mar 02 08:09:39 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 2 Mar 2000 01:20:01 -0800 (PST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A modern substitute for elder pith is expanded polystyrene foam.
The dense EPS strips sold by Diatome for cleaning diamond knives
are excellent for sectioning very small items like Arabidopsis
stems.
Chris Jeffree

} From: Gordon Couger {gcouger-at-rfdata.net}
To: Jon Krupp {jmkrupp-at-cats.ucsc.edu} , Microscopy-at-sparc5.microscopy.com,
Greg Erdos {gwe-at-biotech.ufl.edu}


Dear Jon,
I have had great success using LR White Medium grade methacrylate for such
specimens as iris ovules, mistletoe/host tissues, and a variety of young
plant material. Although time consuming, these tissue were processed with
osmium tetroxide, rinsed in buffer, and exposed to a 0.025% tannic acid
buffer to retain lipids, starch granules, and other items frequently lost
during dehyration in EtOH.

One other advantage is the variety of staining procedures one can use with
material embedded in LR White. Furthermore, in addition to lipid
stabilization, osmium and tannic acid provide additional contrast to the
sections.

Hope this helps. Any questions? Email me at tiekotte-at-up.edu

Cheers!
-Ken

----------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97303

On Wed, 1 Mar 2000, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}



From daemon Thu Mar 02 08:32:19 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Thu, 2 Mar 2000 08:03:37 -0600
Subject: RE: TEM polymer help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} trying to
} visualize the ruthenim doped into a polymer. I have not had much luck...
}
Scott,
Sounds like a job for cryo-ultramicrotome thin-sectioning at approximately
the glass transition temperature of the polymer. This then to be followed
by TEM imaging and EDS analysis of thin-sections, or FESEM-EDS examination
of the "faced-off" polymer block cross-section.

Many rubbery polymers can be stained "enblock" before sectioning, for
example by submersion in a dilute ruthenium tetroxide (RuO4) solution. This
"staining" often aids the sample prep, by hardening the polymer (actually it
initiates some cross-linking) and allowing better sectioning and sometimes
actually enabling ambient microtomy. It sounds like you will not be able to
make use of this technique since you are looking for the Ru distribution in
the polymer.

A great polymer reference is the book, Polymer Microscopy, by Linda Sawyer
and David Grubb (Chapman and Hall).
Good Luck!
Brad Huggins
BP Amoco, Naperville, IL
Microscopy and Microanalysis Lab
630 420-3668

} ----------
}
} From: Scott Whittaker[SMTP:sdw-at-biotech.ufl.edu]
} Sent: Wednesday, March 01, 2000 4:12 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM polymer help
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a set of samples giving me fits here. This person is trying to
} visualize the ruthenim doped into a polymer. I have not had much luck
} working with this stuff and was wondering if any of you had any
} suggestions
} to pass along. A description of the sample follows.Here is what I have
} tried so far.
}
} She originally brought this in on a glass slide and it is about 100µm
} thick. I tried to use backscattering to visualize the Ru metal in the
} FESEM. She thought it might be in little islands. No luck.
} It was too thick to shoot a beam through with the TEM so I embedded some
} in
} cold LR-White resin and sectioned. It offered enough support to get some
} areas thin enough to view through, but I did not see any metal. I
} suggested
} she take the grids to the Materials EM unit here on campus as the have EDS
}
} and are better equipped for this, but they saw nothing either.
}
} I then had her try to spin coat slids,grids,and mica to float off the
} film,
} but she cannot get it to work so now I am back to the slides, scraping
} material off, and cold embedding. The last set was too rubbery though and
} even in epoxy on a good diamond I was unable to obtain a section of the
} material. It just moved out of the way and popped back up with nice resin
} sections floating away. I don't get any polymers here and we are almost
} exclusively a biologic unit so do any of you have any suggestions??
}
}
} } Date: Wed, 1 Mar 2000 11:28:21 -0500 (EST)
} } From: Joanne Bedlek {bedlek-at-chem.ufl.edu}
} } To: Scott Whittaker {sdw-at-biotech.ufl.edu}
} } Subject: Re: your mail
} }
} } Scott,
} }
} } The polymer is a polydimethylsiloxane. The metal (Ru) is dissolved in
} } methylene chloride and added to the silicone fluid. As the polymer cures
} } it "hardens" to a rubbery material.
} }
} } The TEM images you took give us an idea of the polymer distribution.
} With
} } the new films I have, we are interested in the polymer distribution
} again.
} } I
} } suspect we will see the same droplet pattern as before. This will be a
} } characterization technique for a paper submission.
} }
} } As for the dispersion technique, MAIC tried EDS. They were not able to
} } detect the Ru. They did tell me that there is a higher content of Si on
} } the outer sides of the droplets as opposed to the interior areas. This
} is
} } interesting.
} }
} } I will drop off the new samples either next week or the week after. It
} } depends when I get back next Friday.
} }
} } I redid the one sample that was giving you so much trouble in slicing. I
} } hope it has cured better this time.
} }
} }
} } } This may be more of a dispersion thing. Materials should be able to
} see it
} } } if it is in there. You might get with them and see if they have done
} } } anything similar as we had no luck with that last set you brought in.
} I
} } } could also post a discussion to the microscopy listserver and see what
} } } comes up. I just need to know more about the composition of the
} samples
} } and
} } } stuff like that
} } }
}
}
}
}
}
} } {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
} GO GATORS
} Scott D. Whittaker 218 Carr Hall
} EM Technician Gainesville, FL 32610
} University Of Florida ph 352-392-1184
} ICBR EM Core Lab fax 352-846-0251
} sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
} The home of " Tips & Tricks "
}
}
}
}
}
}



From daemon Thu Mar 02 19:17:08 2000



From: Gilbert, Charles :      CGilbert-at-carolinas.org
Date: 2 March, 2000 Time: 7:55 AM E-mail:
Subject: bio: peptidoglycan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We used High- and Low-iron diamine staining in our lab for looking at
glycosaminoglycans several years ago. This is principally EM level. They
can be enhanced with Thiocarbohydrazide-silver proteinate staining. I still
have the protocols that we used. My reference database is at another
location but the ones below should help you get an idea of what is involved
and who did the work. For HID/LID check into the work by Sam Spicer, I
believe he pioneered it. For TCH-SP, the work is by Thiery and speaking
French would be a plus or Sannes.

Hope this helps

Chuck


REFERENCESREFERENCESREFERENCES FOR IRON DIAMINE AND TCH-SP STAINING:

IRON DIAMINE STAININGIRON DIAMINE STAININGIRON DIAMINE STAINING:

Gad, Adel, and B. Sylven. On the nature of the high iron diamine
method for sulfomucins. J Histochem Cytochem. 17:156-60 (1968).

Lev and Spicer. Am. J. Pathol. 46:23 (1965).

Sorvari, Tapani E. Histochemical observations on the role of ferric
chloride in the high-iron diamine technique for localizing sulphated
mucosubstances. Histochem. J. 4:193-204 (1972a).

Sorvari, Tapani E. Binding of ferric ions to nuclei and other tissue sites
in sections stained for sulfated mucosubstances by the high-iron diamine
methods. Stain Technol. 47:245-48 (1972b).

Sorvari, T. E. and H. S. Arvilommi. Some chemical, physical, and
histochemical properties of three diamine fractions obtained by gel
chromatography from the high-iron diamine staining solution used for
localizing sulphated mucosaccharides. Histochem. J. 5:119-30 (1973).

Spicer, S. S. The use of various cationic reagents in histochemical
differentiation of mucopolysaccharides. Am. J. Clin. Pathol. 36:393-407
(1961).

Spicer, S. S. Diamine methods for differentiating mucosubstances
histochemically. J Histochem Cytochem. 13:211-34 (1965).

Spicer, S. S., et al. Ultrastructural visualization of sulphated complex
carbohydrates in blood and epithelial cells with the high-iron diamine
procedure. Histochem. J. 1O:435-52 (1978).

Spicer, S. S. and M. H. Jarrel. Histochemical reaction of an aromatic
diamine with acid groups and periodate engendered aldehydes in
mucopolysaccharides. J Histochem Cytochem. 9:368-79 (1961).

Takagi, Minoru, et al. Ultrastructural localization of acidic
glycoconjugates with the low iron diamine method. J Histochem Cytochem.
30:471-6 (1982).


TCH-SP ENHANCEMENTTCH-SP ENHANCEMENTTCH-SP ENHANCEMENT:

Denys, Francis R., et al. An improved technique to enhance
high-iron diamine reactive sites with thiocarhohydrazide-silver proteinate.
J. Nihon Univ. Sch. Dent. 25(2):103-6 (1983).

Sannes, P.L., et al. Ultrastructural localization of sulfated
complex carbohydrates with a modified iron diamine procedure. J. Histochem.
Cytochem. 27:1108-11 (1979).

Takagi, M., et al. J. Histochem. Cytochem. 30:1179 (1982).

Thiery, J.P. Mise in evidence des polysaccharides sur coupes fines en
microscopie electronique. J. Microscop. 6:987-1018 (1967).

-------------------------------------
Name: Charles Gilbert VOC: (704) 355-5261
Carolinas Medical Center FAX: (704) 355-8424
Dept of Pediatric Research digPager: (704) 355-4088 : 2058
PO Box 32861
Charlotte, NC 28232-2861



Does anyone know of a way to label for peptidoglycan at the light or
electron microscope level?

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu {mailto:billEMac-at-cc.usu.edu}
435-797-1920



From daemon Thu Mar 02 19:17:09 2000



From: Jochonia Nxumalo :      jnxumalo-at-chipworks.com
Date: Thu, 2 Mar 2000 09:57:56 -0500
Subject: FT-IR Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I am new in this discussion groud, my background is in scanning probe
microscopy and mass spectroscopy. At this time I am interested in analysing
plastic materials such as those used in semiconductor packaging. I would
like to use FT-IR. Is this an appropriate direction to take given that some
of my samples are going to be small (tens micrometers). What are the
material limitations of the technique.
Thank you in advance
Jocho


Jochonia N. Nxumalo
Material Science Engineer

Chipworks Inc.
3685 Richmond Road, Suite 500, Ottawa, Ontario, K2H 5B7
Tel:(613) 829-0414 Fax:(613) 829-0515
mailto:jnxumalo-at-chipworks.com



From daemon Thu Mar 02 19:17:09 2000



From: Dave Ward :      dave_ward-at-baxter.com
Date: Thu, 2 Mar 2000 08:07:49 -0800
Subject: Sputter/Cryo Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone is need of an Emscope 2000 sputter/cryostage system at an auction
price please see ad #42220 at LabX.com.



From daemon Thu Mar 02 19:17:10 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 2 Mar 2000 09:37:33 -0800 (PST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
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Dear Professor Luchtel
My apologies for the confusion - I was writing without reference to
Diatome's literature. EPS is just short for Expanded PolyStyrene
but their polystyrol rods would be the same thing.
I have found these Diatome rods useful for a number of
applications, and that their density, which is a little higher than
usual for commercial polystyrene foam, provides good support to
the specimen when hand sectioning. However, if you want to try it
out on the cheap, why not cut strips off an insulated polystyrene
box or insulation tile.

By the way, the best type of blade for hand sectioning used to be
the Blue Gillette double-edged carbon steel razor blades, which
many of you may be too young to know about. The new, doubtless
improved, stainless steel type never seems to be so sharp, and
their edges dull faster. Is there still a manufacturer of carbon steel
razor blades left in the world, or am I doomed to mourn forever?

Good luck
Chris Jeffree

Date sent: Thu, 2 Mar 2000 07:56:53 -0400
To: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} From: Dan Luchtel {dluchtel-at-u.washington.edu}



}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jon -

After you get the stem supported (you've gotten lots of good suggestions),
try taping 2 razor blades together, with a thin spacer (thick paper,
filecard, etc.). Cheaper than a vibratome...

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Thu Mar 02 19:17:14 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 02 Mar 2000 10:24:45 -0800
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ken,
You've spurred a question that is related, yet not exactly on the same
track. Does tannic acid affect the antigenicity of the tissue? I am just
about to embed some tissue in LR White for immunocytological studies, and
though it is established that osmium is a problem for immuno work, I have
heard nothing about tannic acid. I would love to find a way to enhance the
contrast of my tissue, as it often has a surreal, ghostly appearance.
Thanks,
Kristen

At 01:20 AM 3/2/2000 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Mar 02 19:17:15 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Thu, 02 Mar 2000 13:19:40 -0600
Subject: SEM of DNA array

Contents Retrieved from Microscopy Listserver Archives
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I received a sample of DNA microarray on a glass slide and I was asked to do
SEM because the investigator wants to see single and double stranded DNA.
Does anyone know if this is possible. My previous exposure to EM of DNA
samples was in TEM. I would appreciate any help, comments or suggestions.

Thank you,

Corazon D. Bucana
*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Thu Mar 02 19:17:27 2000



From: erich-at-ento.csiro.au (Eric Hines)
Date: Fri, 3 Mar 2000 11:36:49 +1100
Subject: surplus old equipt - edax

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

The following equipment is surplus to requirements and is available on the
basis that you are responsible for all removal and transport costs. If
there are competing bids they will be sold to the highest bidder. There is
no guarantee that the equipment is in operational or serviceable condition
although, to the best of our knowledge, it was functional when last
operated.


Kevex-ray 5100 spectrometer.
Kevex-ray detector 3203-100-VS/30
Kevex-ray detector 3203-100/30-V

Jeol high resolution SEM attachment for 100C(X).

Balzers freeze fracture/etch (~20y/o) "divers bell" chamber - EVM052
controller model.

Eric Hines
Microscopy Centre
CSIRO Entomology and Plant Industry
Canberra, Australia





From daemon Fri Mar 03 08:20:59 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Thu, 2 Mar 2000 22:34:16 -0600
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} By the way, the best type of blade for hand sectioning used to be
} the Blue Gillette double-edged carbon steel razor blades, which
} many of you may be too young to know about. The new, doubtless
} improved, stainless steel type never seems to be so sharp, and
} their edges dull faster. Is there still a manufacturer of carbon steel
} razor blades left in the world, or am I doomed to mourn forever?

I miss the old blades as well. I cured the shaving problem I quit.
For hand sections I have better luck with a more rigid blade.
Razor blades and straight razors tend to dig in for me. I have
a lot better luck with a 2.5 inch wood chisel sharpened on glass
with silicone carbide grit. It is a lot of trouble to get sharp but
it works better for me than razor blades.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri Mar 03 08:55:51 2000



From: Grazyna M Tokarczyk :      gmtokarc-at-is.dal.ca
Date: Fri, 3 Mar 2000 10:26:10 -0400 (AST)
Subject: Re: hand sectioning - carbon blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Thu, 2 Mar 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
} Yes, carbon blades are still made - one of the makers is a Japanese
company - I have a box in my hand, but unfortunately I can't read Japanese
- the only English words on it is "Feather". There may also be many other
makers of these in Asia and Eastern Europe.

Grazyna Tokarczyk
gmtokarc-at-is.dal.ca
Dalhousie University
Halifax, Canada
}
} ----- Original Message -----
} } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} } By the way, the best type of blade for hand sectioning used to be
} } the Blue Gillette double-edged carbon steel razor blades, which
} } many of you may be too young to know about. The new, doubtless
} } improved, stainless steel type never seems to be so sharp, and
} } their edges dull faster. Is there still a manufacturer of carbon steel
} } razor blades left in the world, or am I doomed to mourn forever?
}
} I miss the old blades as well. I cured the shaving problem I quit.
} For hand sections I have better luck with a more rigid blade.
} Razor blades and straight razors tend to dig in for me. I have
} a lot better luck with a 2.5 inch wood chisel sharpened on glass
} with silicone carbide grit. It is a lot of trouble to get sharp but
} it works better for me than razor blades.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}
}
}
}



From daemon Fri Mar 03 08:55:51 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Fri, 3 Mar 2000 08:30:40 -0600
Subject: Balzers 301 freeze fracture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please post the message below.

Dear all,
We have a complete Balzers 301 freeze fracture unit with thickness monitor,
many ancillary accessories and spare parts that is in need of a new home.
No guarantees that the equipment is in operational or serviceable condition
but, to the best of our knowledge, it was functional when last used.
 Please contact me directly for additional information.
Cheers, Mark **************************************************** Mark A.
Sanders
           University of
Minnesota Program Director
          Twin Cities
Campus Imaging Center
            St.
Paul, MN  55108 23-35 Snyder Hall
         ph:
 612-624-3454 1475 Gortner Ave.
         fax: 612-624-1799
http://biosci.umn.edu/imagingcenter President: Minnesota Microscopy Society
http://resolution.umn.edu/MMS/




From daemon Fri Mar 03 08:55:51 2000



From: Pierre-Yves Sizaret :      sizaret-at-med.univ-tours.fr
Date: Fri, 3 Mar 2000 08:33:29 -0600
Subject: confocal

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,
We are in the process to get a confocal microscope for a multi-user
facility. We are not decided yet between different apparatus such as
Wallac ultraview, zeiss LSM 510 or Biorad.
We would very much appreciate any advice helping us for this choice.

Many thanks.

Pierre-Yves Sizaret
Electron Microscopy Facility
University of Tours
France




From daemon Fri Mar 03 17:47:00 2000



From: David_Bell-at-Millipore.com
Date: Fri, 3 Mar 2000 11:02:50 -0500
Subject: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Colleagues,

Twice now, I have had customers in Europe refer to a technique as REM. The
sample prep sounds the same as SEM, and the first time I passed it off as a
typo. Now, with this second reference, I'm not so certain. Is it possible
that these customers are referring to SEM, possibly with their native
language? Or is it a whole different technique/instrument? Could someone
please help me clear up this confusion?

Thanks in advance,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108



From daemon Fri Mar 03 17:47:02 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Fri, 3 Mar 2000 11:56:33 -0500
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Raster Elektronen Mikroskopie = Scanning Electron Microscopy

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Robert J. Palmer Jr., Ph.D.
Oral Infection and Immunity Branch
National Institute of Dental and Craniofacial Research
Bldg. 30, Room 308
National Institutes of Health
Bethesda MD 20892
Ph 301-594-0025
FAX 301-402-0396




From daemon Fri Mar 03 17:47:03 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Fri, 3 Mar 2000 12:31:25 -0500
Subject: Fluorescent agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone heard of a fluorescent agar or a way to make agar fluorescent?
We want to make sure the agar can be fluorescently imaged, but the dye
cannot diffuse out into the surrounding media. Thanks- Dave


************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Fri Mar 03 17:47:06 2000



From: Anita Garg :      Anita.Garg-at-lerc.nasa.gov
Date: Fri, 3 Mar 2000 13:55:09 -0500
Subject: Re: Composition analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues
In a microstructurally uniform alloy, can one expect to get the
chemical composition of the alloy through microprobe (assuming all
the corrections are applied correctly)? Would the composition tally
with the one determined by chemical analysis?
TIA
Anita



From daemon Fri Mar 03 17:47:06 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Fri, 03 Mar 2000 10:55:23 -0800
Subject: confocal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Confocal issues are discussed on another listserver--see below

The best solution is to get one of each manufacturer's confocal systems!
Each one has problems and each one has solutions. In my experience the
Wallac system is not sensitive enough for some of our samples but is great
for live imaging. The BioRad 1024 is difficult because of their interface
to the Nikon TE300 and their use of an old OS2 operating system (the
migration to Windows NT is still in the future) but they have a good
service organization in our area and there are many other users that can
help with advice. I do not have hands on experience with the Leica, Zeiss,
Noran and other systems. They each have attractive features. A recent
discussion of Zeiss and Leica will be sent directly to you. Make a list of
features/advantages/disadvantages and weight them--the results may surprise
you and guide your decision.

to subscribe to the confocal newslist send an email to:

{LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU}

in the body of the message write:

SUBSCRIBE CONFOCAL your name


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Fri Mar 03 17:47:07 2000



From: David_Bell-at-Millipore.com
Date: Fri, 3 Mar 2000 14:20:07 -0500
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi all,

Thanks for a quick and consice response!!

David



From daemon Fri Mar 03 17:47:09 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Fri, 3 Mar 2000 12:38:51 -0700
Subject: RE: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's exactly the same thing:

Scanning Electron Microscopy (English)
RasterElektronenMikroskopie (German)

(It's 3 long words in English, one monumental word in German. I once
gave a talk titled: "Rasterelektronenmikroskopische
Untersuchungsmethoden fuer prozessinduzierte Halbleiterdefekte"....
German is not for the timid ;-)

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From:
"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com[SMTP:"DAVID_BELL-at-MILLIP
ORE.COM"-at-SPARC5.MICROSCOPY.COM]
} Sent: Friday, March 03, 2000 9:02:50 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Question Re: REM
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello Colleagues,

Twice now, I have had customers in Europe refer to a technique as REM.
The
sample prep sounds the same as SEM, and the first time I passed it off
as a
typo. Now, with this second reference, I'm not so certain. Is it
possible
that these customers are referring to SEM, possibly with their native
language? Or is it a whole different technique/instrument? Could
someone
please help me clear up this confusion?

Thanks in advance,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108



From daemon Fri Mar 03 17:47:12 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 3 Mar 2000 12:47:58 -0800 (PST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For those who are 'hand sectioning', try the teflon coated single edged
blades. I believe Ted Pella and EMS carry them. They are alittle more
money, but they slide through tissue with great ease.

Cheers,
Ken

On Thu, 2 Mar 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} ----- Original Message -----
} } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} } By the way, the best type of blade for hand sectioning used to be
} } the Blue Gillette double-edged carbon steel razor blades, which
} } many of you may be too young to know about. The new, doubtless
} } improved, stainless steel type never seems to be so sharp, and
} } their edges dull faster. Is there still a manufacturer of carbon steel
} } razor blades left in the world, or am I doomed to mourn forever?
}
} I miss the old blades as well. I cured the shaving problem I quit.
} For hand sections I have better luck with a more rigid blade.
} Razor blades and straight razors tend to dig in for me. I have
} a lot better luck with a 2.5 inch wood chisel sharpened on glass
} with silicone carbide grit. It is a lot of trouble to get sharp but
} it works better for me than razor blades.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}
}
}
}



From daemon Fri Mar 03 17:47:16 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 03 Mar 2000 15:54:34 -0600
Subject: Re: Composition analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In short, certainly!

The issue might only be are all the elements "visible" to the x-ray
analyzer. I don't trust the C or N numbers that my system spits out. Since
I am using EDS, I will usually trust my careful analyses to a few tenths of
a percent. I can see down to about a tenth of a percent, but the relative
errors are large. But it is usually well sufficient for determining the alloy.

Now just because the alloy is microstructurally uniform, do not count on
the composition to be uniform. I have seen zoning in grains and changes in
composition with length within single crystal ingots. And if you have
multiphase microstructure, it can be challenging to determine to overall
composition.

Warren S.

At 01:55 PM 3/3/2000 -0500, you wrote:

} Dear Colleagues
} In a microstructurally uniform alloy, can one expect to get the chemical
} composition of the alloy through microprobe (assuming all the corrections
} are applied correctly)? Would the composition tally with the one
} determined by chemical analysis?
} TIA
} Anita



From daemon Sat Mar 04 09:11:38 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 3 Mar 2000 17:49:53 -0600
Subject: RE: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In reply to David Bell's inquiry, I know some Eujropeans refer to an SEM as
a Raster Electron Microscope.

Sam Purdy
National Steel Technical Center
Trenton MI


} ----------
} From: "David_Bell-at-Millipore.com"-at-sparc5.microscopy.com
} Sent: March 2000 11:02 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Question Re: REM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Colleagues,
}
} Twice now, I have had customers in Europe refer to a technique as REM.
} The
} sample prep sounds the same as SEM, and the first time I passed it off as
} a
} typo. Now, with this second reference, I'm not so certain. Is it
} possible
} that these customers are referring to SEM, possibly with their native
} language? Or is it a whole different technique/instrument? Could someone
} please help me clear up this confusion?
}
} Thanks in advance,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108
}
}




From daemon Sat Mar 04 09:11:38 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 3 Mar 2000 17:49:37 -0600
Subject: RE: hand sectioning - carbon blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have in my hot little hand a box of carbon steel single edge razor blades
from Ted Pella. For the few times that I needed to to make a transparent
section (being a LM metallographer), I found these razor blades to be
effective.
Usual disclaimers

Sam Purdy
} ----------
} From: Grazyna M Tokarczyk
} Sent: March 2000 926  
} To: Gordon Couger
} Cc: c.jeffree-at-ed.ac.uk; Dan Luchtel; microscopy-at-sparc5.microscopy.com
} Subject: Re: hand sectioning - carbon blades
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} On Thu, 2 Mar 2000, Gordon Couger wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} } Yes, carbon blades are still made - one of the makers is a Japanese
} company - I have a box in my hand, but unfortunately I can't read Japanese
} - the only English words on it is "Feather". There may also be many other
} makers of these in Asia and Eastern Europe.
}
} Grazyna Tokarczyk
} gmtokarc-at-is.dal.ca
} Dalhousie University
} Halifax, Canada
} }
} } ----- Original Message -----
} } } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} } } By the way, the best type of blade for hand sectioning used to be
} } } the Blue Gillette double-edged carbon steel razor blades, which
} } } many of you may be too young to know about. The new, doubtless
} } } improved, stainless steel type never seems to be so sharp, and
} } } their edges dull faster. Is there still a manufacturer of carbon steel
} } } razor blades left in the world, or am I doomed to mourn forever?
} }
} } I miss the old blades as well. I cured the shaving problem I quit.
} } For hand sections I have better luck with a more rigid blade.
} } Razor blades and straight razors tend to dig in for me. I have
} } a lot better luck with a 2.5 inch wood chisel sharpened on glass
} } with silicone carbide grit. It is a lot of trouble to get sharp but
} } it works better for me than razor blades.
} }
} } Gordon
} }
} } Gordon Couger gcouger-at-couger.com
} }
} } Stillwater, OK www.couger.com/gcouger
} } 405 624-2855 GMT -6:00
} }
} }
} }
} }
}
}




From daemon Sat Mar 04 09:11:42 2000



From: MICROFAB-at-aol.com
Date: Fri, 3 Mar 2000 19:39:15 EST
Subject: LM - McArthur Blood Cell Counter

Contents Retrieved from Microscopy Listserver Archives
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I am looking for information on an instrument that I recently acquired. The
item is a McArthur Blood Cell Counter, which is a McArthur portable
microscope with a number of mechanical additions and a special stage,
apparently for doing blood count. It is in a neatly bundled case, very
portable, but lacking instructions. If anyone one is familiar with the
specific application of the set I would appreciate comments, particularly if
you have ever used such an instrument.

Jim Harper
microfab-at-aol.com



From daemon Sat Mar 04 09:11:44 2000



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Sat, 4 Mar 2000 09:15:46 +0800
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
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There's also the technique of Reflection Electron Microscopy, which is the
imaging analogue of RHEED (reflection high energy electron diffraction). I
am sure your reference is to the SEM though, as the other respondents
indicate.

Just for interest, REM (like RHEED) is a surface sensitive technique and can
give beautiful images of monolayer surface steps, eg on the sapphire
surface, and this can all be done in the TEM. The sample is simply rotated
90 degrees from the usual TEM imaging position and so electrons are
'reflected' from the surface at glancing angle as they pass through the
sample chamber.

My best wishes to all,

Mark

%%%%%%%%%%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119602

TEL: (65) 874 8591
FAX: (65) 872 0785
email: m-yeadon-at-imre.org.sg


-----Original Message-----
} From: "David_Bell-at-Millipore.com"-at-sparc5.microscopy.com
To: "David_Bel-at-Millipore.com"-at-ultra5.microscopy.com
Cc: Microscopy-at-sparc5.microscopy.com
Sent: 3/4/00 3:20 AM



Hi all,

Thanks for a quick and consice response!!

David



From daemon Sun Mar 05 10:29:46 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 5 Mar 2000 23:49:38 +1000
Subject: RE: Question Re: REM, History Note

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The original question has been answered well. However, I would like to add the
historical footnote that von Ardenne's seminal 1938 paper was entitled "Das
Elektronen-Rastermicroskop". A raster is a pattern of horizontal lines. The
German word is derived form the Latin "rake". I guess a rake's pattern is a
raster, a bit coarse for an SEM though.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, March 04, 2000 2:03 AM,
"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com
[SMTP:"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com] wrote:
}
}
} Hello Colleagues,
}
} Twice now, I have had customers in Europe refer to a technique as REM. The
} sample prep sounds the same as SEM, and the first time I passed it off as a
} typo. Now, with this second reference, I'm not so certain. Is it possible
} that these customers are referring to SEM, possibly with their native
} language? Or is it a whole different technique/instrument? Could someone
} please help me clear up this confusion?
}
} Thanks in advance,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108
}



From daemon Mon Mar 06 08:12:12 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:09:06 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul,

I have recently encountered several mentions of this type of thing
recently, in the oddest places. One was a presentation by the motivational
speaker, Tony Robbins, who, in front of 13,000 people at the Hartford Civic
Center, described how his recent feelings of fatigue and lack of energy had
been diagnosed by darkfield microscopy as due to chlamydia in his blood.
He cited work done by Dr. Robert Young at InnerLight.

As for crystal testing, I am still a strong supporter of Polarized light
work for that. (Interestingly, it looks a lot like darkfield and, perhaps,
is mis-cited by Naturpaths who might not know the difference).

Hope this is helpful.

Caveat: MME has no connection with either Mr. Robbins, Dr. Young, or
InnerLight.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



At 06:33 PM 3/1/00 -0600, pfieber-at-elite.net"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:28:03 -0500
Subject: Re: SEM of DNA array

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Corazon,

We did a bit of market and applications research into this area for the 3
years before it emerged (as well as launching a company). These
microarrays are typically analyzed on larger scale using instruments which
are derivatives of confocal. If you are looking for individual DNA
molecules first make sure that they are really likely to be there (i. e,
get a full history of how this microarray was prepared and later used). I
would then suggest looking at it with AFM. ThermoMicroscopes, Digital,
and Molecular Imaging have all had experience in this area. Let me know if
you need specific contact info.

Best regards
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 01:19 PM 3/2/00 -0600, Corazon Bucana wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:44:15 -0500
Subject: Re: LM - McArthur Blood Cell Counter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jim,

Not only have I had my hands on several McArthurs, I had the privilege of
visiting the inventor at home. Your comment about the "neat bundle" is
very accurate. Dr. McArthur showed me a number of accessories (including
fluorescence!) for this system. Although I did not have a chance for an
extended use, my recollection was that all of them worked passably well.

There were several generations of McArthur microscopes. The early ones
were made of metal and were very durable and usable. A somewhat later
generation was made of white plastic, I think for the BBC Open University.
The general opinion of some of my colleagues who knew this system indicated
that the earlier generation was a better bet.

I was not aware that anyone was commercially making this microscope today.
Dr. McArthur was advanced in years when I visited him over a decade ago
and, at that time, their construction was very much a cottage industry. Is
this a new system or are you buying it second hand? (I suspect the latter,
since you mentioned that the instructions are missing). It is hard to
comment on the bits and pieces without further information. I am planning
to attend a number of meetings (Experimental Bio, M&M, Cell Bio, Neuro as
well as Semicon West, Materials Solutions, and ISTFA) over the next 6
months. If you are going to any of them or will be in the area, I would be
delighted to take a look at what you have and see if I can dredge up any
old memories of how it works.

Finally, if you can send pictures, I can forward them to colleagues in the
UK who own McArthurs.

Hope this is helpful.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}

At 07:39 PM 3/3/00 EST, MICROFAB-at-aol.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:15 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:48:10 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul,

I have recently encountered several mentions of this type of thing
recently, in the oddest places. One was a presentation by the motivational
speaker, Tony Robbins, who, in front of 13,000 people at the Hartford Civic
Center, described how his recent feelings of fatigue and lack of energy had
been diagnosed by darkfield microscopy as due to chlamydia in his blood.
He cited work done by Dr. Robert Young at InnerLight.

As for crystal testing, I am still a strong supporter of Polarized light
work for that. (Interestingly, it looks a lot like darkfield and, perhaps,
is mis-cited by Naturpaths who might not know the difference).

Hope this is helpful.

Caveat: MME has no connection with either Mr. Robbins, Dr. Young, or
InnerLight.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



At 06:33 PM 3/1/00 -0600, pfieber-at-elite.net"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

[message truncated]




From daemon Mon Mar 06 08:12:19 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 05 Mar 2000 16:03:41 -0600
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Amazing. Definitely a major feat of dark field light microscopy.
To be able to see and distinguish an organism that is between
0.1u and 0.5u is truly amazing.

Did he say what magnification was realized in this DF system?
The only DF condensers I know of for compound LMs are
not aplanatic. So is begs the question of how one could
resolve, much less see, such a tiny bacteria using LM.

Perhaps the sample was specially treated?

gary g.



At 01:09 PM 3/5/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:31 2000



From: Gerhard Frank :      frank-at-ww.uni-erlangen.de
Date: Mon, 06 Mar 2000 10:05:06 +0100
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


REM = 'Raster Elektronen Mikroskopie' in German (SEM in english) but
also
Reflection Electron Microscopy, which also needs not a lot of specimen
preparation. It looks very much like preparation for SEM in deed.
Regards
Gerhard Frank

"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Colleagues,
}
} Twice now, I have had customers in Europe refer to a technique as REM. The
} sample prep sounds the same as SEM, and the first time I passed it off as a
} typo. Now, with this second reference, I'm not so certain. Is it possible
} that these customers are referring to SEM, possibly with their native
} language? Or is it a whole different technique/instrument? Could someone
} please help me clear up this confusion?
}
} Thanks in advance,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108

--
Gerhard Frank
UNIVERSITAET ERLANGEN-NUERNBERG Phone: +49 9131 85 28606
Institut fuer Werkstoffwissenschaften VII Fax: +49 9131 85 28602
Cauerstr. 6, D-91058 Erlangen, Germany frank-at-ww.uni.erlangen.de



From daemon Mon Mar 06 08:12:37 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 06 Mar 2000 08:32:11 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

Just a reminder that darkfield is detection limited, not resolution
limited. It only takes 2-3 photons, scattered from a feature, to indicate
that it is there.

Chlamydia is must be much bigger than the range you specified. We are in
the process of sending out a special mailer for a new spectral imaging
system, with chlamydia as the "cover shot". I think that this image was
acquired with a 40x objective, but will check. Data is intentionally not
provided on the card because we are encouraging recipients to go to the web
page.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 04:03 PM 3/5/00 -0600, Dr. Gary Gaugler wrote:
} Amazing. Definitely a major feat of dark field light microscopy.
} To be able to see and distinguish an organism that is between
} 0.1u and 0.5u is truly amazing.
}
} Did he say what magnification was realized in this DF system?
} The only DF condensers I know of for compound LMs are
} not aplanatic. So is begs the question of how one could
} resolve, much less see, such a tiny bacteria using LM.
}
} Perhaps the sample was specially treated?
}
} gary g.
}
}
}
} At 01:09 PM 3/5/00 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:52:19 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 6 Mar 2000 08:32:43 -0600
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
To respond to a comment made by Gary Gaugler, and without in
ANY WAY seeking to validate or support the claims of naturopaths,
darkfield microscopy has imaged individual microtubules, diam 25 nm.
Same with Nomarski optics, and others. How is that possible? It is
important to keep in mind what the famous "resolution limit" means,
it means telling the difference between two and one object. It has
nothing to say about the smallest absolute size of an object that can
be detected.

If you imagine a perfectly black slide with a pinhole in it
made by a perfect iris that can shrink right down to infinitely small
(I did say a perfect iris), there is no limit in theory to how small
that pinhole can be and still be imaged. Once the diameter of the
pinhole becomes less than a certain length, set by diffraction, then
the size of the image of the pinhole will no longer get any smaller.
Instead, the contrast in the image will go down. But if you can put
enough light through and have a good enough detector, you can see
that image.

Going back to our microtubules, take a photo of the darkfield
image and measure the diameter of the microtuble in the image: it
will be around 250 nm or thereabouts.

For the record, the person who I think has published the most
dark field images of microtubules is called Hotani, he works in
Japan, I am not sure where.

Of course, doing this in practice is not easy, but it is
certainly possible.

As ever,
Tobias


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Mon Mar 06 10:02:32 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 6 Mar 2000 10:42:54 EST
Subject: 16 bit image processsing and measurement plugins for Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An earlier thread on this listserver (May-June 1999, and also summarized in
the January/February 2000 issue of Microscopy and Microanalysis) asked about
the availability of software for the processing and analysis of 16 bit per
channel images, which are obtained from flat bed scanners, cooled digital
cameras, and some microscopes (especially AFMs). Following the encouragement
of members of this list, we've written a set of image processing and
measurement routines for these images. Those interested can get information
at http://members.aol.com/FoveaPro/

Disclaimer: Please note that Fovea Pro is a commercial product sold by
Reindeer Games. My connection with the company is through my son, Chris, who
wrote the programs, and my own role in writing the accompanying tutorial.

John Russ



From daemon Mon Mar 06 10:12:31 2000



From: Christine Brantner :      brantner-at-codon.nih.gov
Date: Mon, 6 Mar 2000 11:15:10 -0400
Subject: Tracor Northern 5500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings

We have a Tracor Northern 5500 EDX unit that we would like to give away.
It comes with a manual, mainframe, and monitor. If anyone is interested
please contact:

Dr Brian Andrews
NIH, NINDS
Bethesda MD
301-435-2796

sba-at-helix.nih.gov


Thanks
Chris
:):):):):):):):):):):):):):):):):):):):):):):):):):):):):):)

Christine A. Brantner, Ph.D.

Biologist/Lab Manager

National Institutes of Health
National Institute of Neurological Diseases and Stroke
Lab of Neurobiology
Section of Analytical Cell Biology
Bldg 36, room 2A21 phone 301-435-2803
36 Convent Dr fax 301-480-1485
Bethesda, MD 20892-4062



From daemon Mon Mar 06 10:42:21 2000



From: Nina Allen :      nina_allen-at-ncsu.edu
Date: Mon, 6 Mar 2000 11:39:31 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:
I will add just a few comments to those of Tobias Baskin. When we
first developed Video Microscopy in the early eighties, we were visulizing
microtubules and other structures smaller than the ca. 200 nm resolution
limit using DIC and computer image enhancement. This is discussed in the
early papers from 1981 and 1983 (R.D. Allen and N.S. Allen 1983, J. of
Microscopy),and more easily available in the Inoue and Spring second
edition of Video Microscopy, Plenum Press. The important thing to remember
is that you can visualize items smaller than 100 nm, but you are not
resolving them.
With the new method Polarization Modulation DIC we get very good images of
microtubules and other fine structures (Holzwarth, G., Webb, S.J.,
Kubinski, D.J., N.S. Allen. 1997. Improving DIC Microscopy with
Polarization Modulation. Jour. of Microscopy 188:249-254). Contrast is
doubled with this method and it is worth a try. It is commercially
available from Hamamatsu or you can build your own ,Nina Allen
} Greetings,
} To respond to a comment made by Gary Gaugler, and without in
} ANY WAY seeking to validate or support the claims of naturopaths,
} darkfield microscopy has imaged individual microtubules, diam 25 nm.
} Same with Nomarski optics, and others. How is that possible? It is
} important to keep in mind what the famous "resolution limit" means,
} it means telling the difference between two and one object. It has
} nothing to say about the smallest absolute size of an object that can
} be detected.
}
} If you imagine a perfectly black slide with a pinhole in it
} made by a perfect iris that can shrink right down to infinitely small
} (I did say a perfect iris), there is no limit in theory to how small
} that pinhole can be and still be imaged. Once the diameter of the
} pinhole becomes less than a certain length, set by diffraction, then
} the size of the image of the pinhole will no longer get any smaller.
} Instead, the contrast in the image will go down. But if you can put
} enough light through and have a good enough detector, you can see
} that image.
}
} Going back to our microtubules, take a photo of the darkfield
} image and measure the diameter of the microtuble in the image: it
} will be around 250 nm or thereabouts.
}
} For the record, the person who I think has published the most
} dark field images of microtubules is called Hotani, he works in
} Japan, I am not sure where.
}
} Of course, doing this in practice is not easy, but it is
} certainly possible.
}
} As ever,
} Tobias
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Nina Stromgren Allen
Professor, Department of Botany;
Director, Cellular and Molecular Imaging Facility
Box 7612, Department of Botany
North Carolina State University
Raleigh, NC 27695-7612
Phone: 919-515-8382 (Office), 515-3525 (Lab), 515-2727 (Department Secretary)
Fax: 919-515-3436





From daemon Mon Mar 06 11:32:14 2000



From: Baliga, Vicki L. :      Vicki.L.Baliga-at-pmusa.com
Date: Mon, 6 Mar 2000 12:20:30 -0500
Subject: Need a High Resolution Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone familiar with a printer(s) that can provide equivalent
detail to a polaroid print or to the images that can be collected from the
scanning electron microscopes? I have looked at QMS Color Magic II printers
that have 2400 dpi x 600 dpi, 257 shades of gray, with a cell size of 150
cells per inch (lines per inch). This is comparable to a Codonics 300 line
dye-sub printer that I have seen. However, the dye-sub printers tend to
smear fine details to the point of not being visible. I saw an add for an
Alps printer with a dot size of 10 microns at 2400 dpi. The add did not
indicate the shades of gray so I have no way of knowing what the numer of
cells per inch are. I suspect that it would have 257 shades of gray which
would give a cell size of 16x16 and a cells or lines per inch of 150. Does
anyone have user information about these or any other printers that might
work?
No one talks about the cells per inch (lines per inch) in any of the
documentation. For me, cells per inch has been the best measure of
resolution. For the printers that we have, the extra dots (300 dpi matrix
4x4, 600 dpi matrix 8x8, 1200 dpi matrix 12x12, 2400 dpi matrix 16x16) serve
to provide more dynamic shades of gray. A 300 dpi with 17 shades of gray
and a 600 dpi with 65 shades of gray both have 75 cells per inch. The only
difference is more contrast in the 600 dpi with 65 shades of gray. A simple
formula for this is dpi/cell matrix size= cells (lines) per inch, 2400
dpi/16=150 cells per inch and a maximum # of shades of gray =
1+(dpi/lpi)squared. This tid bit of information came from The Image
Processing Handbook, 2nd ed. by John C. Russ.
I would appreciated any help that I can get in finding a printer
that will do the job. Thanks.

Vicki Baliga
Vicki.L.Baliga-at-pmusa.com
804-274-3088



From daemon Mon Mar 06 12:22:06 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 6 Mar 2000 13:03:10 EST
Subject: Re: Need a High Resolution Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 3/6/00 5:55:31 PM, Vicki.L.Baliga-at-pmusa.com writes:

.."A 300 dpi with 17 shades of gray
and a 600 dpi with 65 shades of gray both have 75 cells per inch. The only
difference is more contrast in the 600 dpi with 65 shades of gray. A simple
formula for this is dpi/cell matrix size= cells (lines) per inch, 2400
dpi/16=150 cells per inch and a maximum # of shades of gray =
1+(dpi/lpi)squared. This tid bit of information came from The Image
Processing Handbook, 2nd ed. by John C. Russ...."

I guess it is worth pointing out that the calculation Vicki has quoted here
is a bit optimistic. Most printers have dots that overlap somewhat, and the
dots may spread a bit on the paper. Plus the dots are large enough to be
resolved by the human eye, which creates interesting effects when they start
to touch or overlap. And the shades of grey that perfect dots might produce
would be linear rather than log, as human vision perceives brightness. It
would be conservative to estimate that a printer that seems technically
capable of producing (e.g.) 65 shades of grey in a given printing cell size
will actually produce images with about half that number of really useful
shades of grey. But that is still OK since that is about all the distinct
shades that a person can detect on a print (and more than most Polaroid
prints have, although the negatives are much better).

Halftone printing is never going to be as good as something that really
covers the resolution cell with a uniform shade of grey (or color). Dye sub
and a few ink jet printers do a much better job than laser printers in that
regard.

John Russ



From daemon Mon Mar 06 14:01:52 2000



From: Catherine Ripley :      ripley-at-aecom.yu.edu
Date: Mon, 06 Mar 2000 14:48:13 -0500
Subject: Confocal Newslist or Listserver??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Larry

I sent an email to LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU as you suggested with
SUBSCRIBE CONFOCAL in the body of the message.

I got this message back:
No LISTSERV list by the name of "CONFOCAL," is known to exist. Note that
lists can be marked "confidential" and that the existence of such lists is
usually known only to the server that is actually hosting it.

I'm very interested in finding a venue that is more oriented toward
confocal microscopy. Any further suggestions?




Catherine Ripley
*****************************************
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461
*****************************************
*****************************************



From daemon Mon Mar 06 18:11:17 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Tue, 07 Mar 2000 10:55:26 +1100
Subject: Microscopy of blood

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists can look at their own blood!

If you take a drop of blood from a pinprick and put it on a slide, you will
see (at rather lower magnification) what the "live cell" analysts purport
to see. You will see red cells starting to agglutinate. Thats all. It is
nearly impossible to find a white cell. If you find a bacterium you are
probably near death. Using dark field adds mystery but no more useful
information.

Check out these websites for three different perspectives on live cell
microscopy


1. http://www.hcrc.org/faqs/livecell.html

Critical evaluation pointing out the supposed features naturopaths claim as
showing pathological change are natural variation.



2. http://www.veg.on.ca/lifelines/janfeb/analysis.htm

A midly critical warning that most Live Cell Analysis practitioners are not
professional pathologists!

3. http://www.tlpdesign.com/hplusw/alt_directory/livecellanalysis.html

Gushing plug for the technique including claiming ability to detect poor
nutrition, vitamin deficiency etc. See the extract below.


None of these claims that illness can be diagnosed by observing
coverslipped blood have any scientific validity at all.

"Live cell analysis can determine cellular nutritional status. Nutritional
status is essential to aid in curbing and reversing the free radical
cascade of destructive, deteriorating cell structures.

This unique analysis of peripheral blood can reveal a good prospectus of
the immune system. By observing the morphology of the white corpuscles and
their activity in contrast to the extent of active foreign antigens and
microbes within the serum and red blood cells, the strength of the immune
system
can be judged. Weakness and dysfunction in any one of these three major
components and influence the strength and function of the other two. This
can be indicative of a progressive disease process."



From daemon Mon Mar 06 18:31:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Mar 2000 16:06:36 -0600
Subject: Re: Need a High Resolution Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A photographic negative and/or print is continuous tone. Digital
printers (ink jet, laser, etc.) attempt to reproduce continuous tone
via modulation of the size of the dots or the number of dots in
an area. Continuous tone digital printers typically use dye sublimation
to produce photographic quality results. In this regard, there really
isn't a true correspondence to dpi. Alternatively, some ink jets use
CMYK or even 5 color jets rather than RGB or RGB+B. Some of the
new generation do a rather good job of color printing.

But since you are talking about SEM images, these are gray scale
images rather than color. So, what are the options for b/w?

600dpi and 1200dpi laser printers do a rather good job of printing
SEM images. One must adjust the toner density to achieve maximum
quality results. But regardless, they are not continuous tone prints.
The closest thing to continuous tone b/w is the Orion (I think that
is the name) printer. It is an effective 300dpi and prints on expensive
paper--which is par for the course, considering the cost of the
printer (about $25K). And the printer is sloooooow.

I use a HP Laserjet 4M Plus and am very pleased with the results.
But the printed images are not archival and are not continuous tone.
But they are very inexpensive.

If you can describe what your actual end purpose is, perhaps others
could offer some good options for you to consider. Are you talking
about proofing, archiving, presentations, etc?

gary g.



At 11:20 AM 3/6/00 , you wrote:
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From daemon Mon Mar 06 22:20:18 2000



From: JUPE Peter :      JUPE.Peter-at-hdh.com.au
Date: Tue, 7 Mar 2000 14:35:46 +1100
Subject: Transmission electron microsocopy

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir
Could you please supply me details on "transmission electron microsocopy"
for my 16yr old daughter at high school, or alternatively where to look on
the Internet.
Thanking you
Peter Jupe



From daemon Tue Mar 07 08:06:11 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 7 Mar 2000 01:29:48 -0800 (PST)
Subject: Splitting hairs or Chylamdiae

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Splitting hairs on detecting microtubules vs. detecting pathogenic
organisms...

I guess I will have a go at this and muddy the waters...

As a background...The inability of Chlamydiae to produce metabolic energy
restricts these obligate 'parasites' to an intracellular existence.

The infectious component (elementary body) is about 3um in diameter, well
within the resolvable range of the OLM. Once acquired in the host cell
through phagocytosis, a vaculated 'initial body' (0.5 -1.0um) is formed:
huge by OLM standards!

'Elementary bodies' and 'initial bodies' possess distinctive staining
properties using Giemsa staining and Macchiavello's staining techniques,
not be mention detection by immunocytochemistry.

One can perhaps differentiate Chlamydia induced inclusion bodies
juxtaposed to the nucleus of the host cell using Darkfield illumination.
However, differential diagnosis of Chylamydia by Darkfield alone is
questionable.

If one considers Darkfield and the ability of a 'substance' of a given
refractive index to scatter light with respect to the R.I. of the adjacent
material, we must accept the notion of "detection vs. resolution' (B.
Foster ' Optimizing Light Microscopy for Biological and Clinical
Laboratories', pg. 57): therefore, we may detect an inclusion body, but
can we identify the pathogen of the inclusion body? Perhaps Phase
Contrast would be more sensitive to refractive differences?

As the devil's advocate, I see the issue of using Darkfield as a definite
diagnostic tool being dilute to 'mine is smaller than yours'. Not being
up on the naturopathic literature, I question how only using Darkfield
Illumination can definitely dx Chlamydia?

However, comparing various organisms and the use of Darkfield
Illumination, 'Treponema pallium' can easily be detected by Darkfield; an
organism of 0.2 um wide, but 5-15 um in length! Furthermore, the
inclusion body of Rabies can easily be detected by Darkfield and
Brightfield; however, we i.d. the total sum of virons: an example of
'detection vs. resolution'.

Yes, we as microscopists scoff at unconventional claims. Perhaps,
Mr. Tim Robbins should present a blood sample for us to examine under
the light of mutual cooperation and enlightenment'!!


I present my premise for your delicate evisceration...

Cheers!

-Ken






From daemon Tue Mar 07 08:06:15 2000



From: John Runions :      cjr41-at-cam.ac.uk
Date: Tue, 7 Mar 2000 11:35:10 +0100
Subject: Re: Confocal Newslist or Listserver??

Contents Retrieved from Microscopy Listserver Archives
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Catherine,

I also tried to subscribe to the confocal list when Larry suggested
it and it worked like a charm. I cut and pasted the email address
right from his posting.

LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU

Try again. Cheers, John


} ------------------------------------------------------------------------
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--
C. John Runions, Ph.D.
Department of Plant Sciences
University of Cambridge
Cambridge CB4 1YE
UK

email at work cjr41-at-cam.ac.uk
email at work runions-at-ntlworld.com
Phone (01223) 766 545



From daemon Tue Mar 07 08:06:17 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 7 Mar 2000 08:48:54 -0500 (Eastern Standard Time)
Subject: Re: Microscopy of blood

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In the earlier days {25 years ago} of medical diagnosis, before the
advent of the rapid, highly specific methods of identifying pathogenic
organisms, darkfield microscopy was routinely applied to rule out
specific organisms. In particular, the diagnosis of syphilis was aided
with this mode of imaging. When fluids from a suspect lesion were
applied to a slide and imaged by darkfield, the characteristic shape of
the spirochaete organisms was clearly seen. More recently, darkfield
examination of synovial fluids from arthritic joints was used to rule
out or diagnose Lyme disease, before specific testing became available.

W. L. Steffens, Ph.D
University of Georgia



From daemon Tue Mar 07 18:06:02 2000



From: rlvaughn-at-unmc.edu
Date: Tue, 7 Mar 2000 17:06:42 -0600
Subject: TEM - ImmunoEM reference

Contents Retrieved from Microscopy Listserver Archives
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Is there a short, general reference, like a review, that explains immuno
EM, it's uses, major techniques, and shortfalls? I would like to use it to
send to investigators that are thinking about using the technique but don't
know much about labeling at the EM level. I have a lot of specific
references and it would take a lot of time to reference or copy them. If
the investigator wants to know more I can direct them to those, otherwise a
general one would get them and me on the same page when we start talking
specifics on their project. Thanks

Rick Vaughn
rlvaughn-at-unmc.edu




From daemon Wed Mar 08 07:36:58 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 08 Mar 00 00:40:11 -0800
Subject: TEM - ImmunoEM reference

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Rick Vaughn writes:
Is there a short, general reference, like a review, that explains immuno
EM, it's uses, major techniques, and shortfalls? I would like to use it to
send to investigators that are thinking about using the technique but don't
know much about labeling at the EM level. I have a lot of specific
references and it would take a lot of time to reference or copy them. If
the investigator wants to know more I can direct them to those, otherwise a
general one would get them and me on the same page when we start talking
specifics on their project. Thanks
Rick Vaughn
rlvaughn-at-unmc.edu

The best reference you can ask for is the 1993 book by Gareth Griffith: "Fine Structure Immunocytochemistry." Springer Verlag. In it you will find all the major techniques, pitfalls, antibody use, fixatives and even quantitative methods for estimating antigen number. My copy has been replaced many times, I think because someone else needed it more than I did!

Paul Webster.




From daemon Wed Mar 08 18:23:47 2000



From: Jaci Lett :      jmlett-at-cid.wustl.edu
Date: Wed, 8 Mar 2000 18:09:50 -0600
Subject: methods of embedding cells cultured on glass coverslips

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I know this has been covered periodically, but as I've not needed the
technique, I've not paid attention.  I need some methods of embedding
cells cultured on glass coverslips.  Obviously, the most important
issue is removing the coverslip from the polymerized block.I've looked
through my own reference materials and the only method I found involved
using Thermanox coverslips (upon which these particular cells will not
grow, I'm informed).Please reply dircectly to my email address as well as
to the Listserver (I haven't received postings for several days). Thank
you,Jaclynn M. Lett, Research Assistant    
jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
MO  63110voice:  314-977-0257     fax: 
314-977-0030




From daemon Wed Mar 08 18:23:47 2000



From: R schlicher :      rkslick-at-yahoo.com
Date: Wed, 8 Mar 2000 18:14:50 -0600
Subject: Rapid Fixation of cells for SEM)

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I need to fix cells for viewing with SEM. However,
the fixation method needs to be consistent with my
experimental methods:

1) I need to fix the cells in less than 1 minute and
due to this time limitation, I cannot spin the cells
into a pellet and must add the cell suspension to the
fixative; and

2)I am looking for "effects" to the cell membrane
caused by my experiment, so I need to keep any
possible fixative damage/effects at a minimum.

I have fixed cells using plunge freezing and viewed
them with TEM, this is time consuming and I ran into
a
lot of freeze damage. I would like to try chemical
fixation and SEM. It has been suggested that I try
glutaraldehyde + buffers, then postfix with osmium
tetroxide and dehydrate in an ethanol series.

Is this the best method? I am open to suggestions.
I am using suspensions of prostate cancer cells in
RPMI (+serum) growth media; I can perform the
experiment in buffered saline and use a high
concentration of cells.

Thanks,
Robyn


Robyn K. Schlicher, M.S.
Laboratory for Drug Delivery
Institute for Bioengineering and Biosciences
Georgia Institute of Technology
315 Ferst Drive
Atlanta, GA 30332
rkslick-at-yahoo.com


__________________________________________________
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Talk to your friends online with Yahoo! Messenger.
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From daemon Wed Mar 08 18:23:47 2000



From: amit kaulgud :      amit_kaulgud-at-hotmail.com
Date: Wed, 8 Mar 2000 18:11:39 -0600
Subject: OsO4 with PTFE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



hi
} i am doing research at university of massachusetts lowell.i am working on
} charaterization of Polytetrafluoroethylene(PTFE).While doing TEM i have
} stained the PTFE with osmium tetroxide.Can any one enlighten me on the
} mechanism oF OsO4 with PTFE?
} thanks
} ________________________________________________
} Amit A.Kaulgud
} Research Assistant.
} University of Massachusetts Lowell
} Department of Chemical/Materials Engineering.
} 170, Riverside Street,#3
} Lowell MA -01854.
} Phone/Voice:978-459-3248.
} email: amit_kaulgud-at-hotmail.com

______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com




From daemon Wed Mar 08 18:23:48 2000



From: Schmitz, Robert :      rschmitz-at-uwsp.edu
Date: Wed, 8 Mar 2000 18:09:05 -0600
Subject: 2.5% glut shelflife

Contents Retrieved from Microscopy Listserver Archives
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Below is a question from one of my former students who is now working in a
hopsital histotech lab. It concern the selflife of glutaraldehyde fixative.
I always mix my fix fresh, so some other perspectives might be useful!!
Here's the Question:

} My boss wants to know how long a working solution of glutaraldehyde
} remains stable (2.5% in cacodyalate buffer). How long can we keep it in
} the refrigerator?
}
Bob
Robert J. Schmitz
Electron Microscope Lab
Department of Biology, CNR Building
University of Wisconsin, Stevens Point
Stevens Point, WI 54481
phone (715) 346-2420
FAX (715)346-3624
email rschmitz-at-uwsp.edu
http://biology.uwsp.edu/faculty/RSchmitz/home.html




From daemon Wed Mar 08 18:53:42 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 08 Mar 2000 16:40:22 -0800
Subject: TEM - ImmunoEM reference

Contents Retrieved from Microscopy Listserver Archives
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In addition, I would point that Paul's web site at
http://www.hei.org/htm/cryo.htm is not bad source, too.

} Date: Wed, 08 Mar 2000 00:40:11 -0800
} From: Paul Webster {pwebster-at-hei.org}
} Subject: TEM - ImmunoEM reference
} To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} Reply-to: Paul Webster {pwebster-at-hei.org}
} X-Mailer: QuickMail Pro 1.5.4 (Mac)
} X-MIME-Autoconverted: from quoted-printable to 8bit by
ultra5.microscopy.com id
} CAA01345
}
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Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 08 20:37:20 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Wed, 8 Mar 2000 20:28:33 -0600
Subject: ESEM Symposium at the Fall MRS

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At the Fall MRS this year there will be a symposium dedicated to
environmental SEM and low vacuum SEM, invited speakers include Eric
Doehne from Getty Conservation Institute, David Joy from the
University of Tennessee and Matthew Phillips from The University of
Technology in Sydney. Contributed papers from all environmental SEM
and low vacuum SEM users are encouraged, the Preliminary Call for
Papers follows:

Preliminary Call for Papers , Session for MRS 2000 (Boston)
http://www.mrs.org/meetings/fall2000/cfp/symposia.html

Low Vacuum SEM/ESEM in Materials Science
"Wet SEM: the Liquid Frontier of Microscopy"

Abstract Deadlines: June 5th for abstracts sent by mail and June 19th
for abstracts submitted via the MRS Web Site (http://www.mrs.org).

Scanning electron microscopy and x-ray microanalysis can now be
performed at pressures as high as 1 - 5000 Pa (~0.01 to 40 torr) in
the class of instruments known variously as "low vacuum", "elevated
pressure", and "environmental" SEMs. This represents an increase of
3 to 6 orders of magnitude in pressure compared to the operating
conditions of ordinary high vacuum SEMs. At the upper end of this
pressure range, water can be maintained in the liquid state at
temperatures from 3 - 10 deg. C. This remarkable situation has
opened up broad new areas of opportunity in many fields, especially
materials science. Dynamic processes can be observed with many of
the familiar strengths of conventional SEM: high resolution, large
depth of focus, and a variety of imaging signals that reveal
compositional, topographic, electrical and other specimen properties.
X-ray microanalysis for elemental characterization is also possible,
although somewhat compromised. Chemical, physical, and mechanical
experiments can readily be performed in user-specified environments.
The microscope can thus be thought of as an appendage to an
experimental chamber. Papers are solicited in all aspects of
materials science in which this new microscopy is used.

Co-organizers:

Dr. Brad Thiel
Polymers & Colloids Group
Cavendish Laboratory
University of Cambridge
Madingley Road
Cambridge, CB3OHE, UK
44 1223 337 272
44 1223 337 000 (fax)
bt202-at-cam.ac.uk

Dr. John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
(734) 936-3352
(734) 763-2282 FAX
jfmjfm-at-engin.umich.edu

Dr. Dale Newbury
Surface and Microanalysis Science Division
National Institute of Standards and Technology
Gaithersburg, MD 20899-8371 USA
301-975-3921
301-417-1321 (fax)
dale.newbury-at-nist.gov


















--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42#161# 16' 48" Long. 83#161# 43' 48"






From daemon Thu Mar 09 02:26:29 2000



From: Kyrill Ukhanov :      ukhanov-at-rz.uni-potsdam.de
Date: Thu, 9 Mar 2000 09:18:59 +0100 (MET)
Subject: fluoronanogold stability

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I have used fluoronanogold for a couple of times and it did not work on the
EM level. Then I realized that this batch has been already expired. Does
anyone know if upon aging fluoronanogold could lose gold leaving only FITC
molecule attached to Ig? I did see a fluorescence signal before running
silver enhancement though. Currently I will try regular ultrasmall gold
probe to clear up this problem but I wonder what was the trick.

thanks for your help

Kyrill

**********************
Dr. Kyrill Ukhanov
Institute for Zoophysiology, University of Potsdam,
Lennestrasse 7a, D-14471 Potsdam, Germany
phone +49-0331-9774859
fax +49-0331-9774861



From daemon Thu Mar 09 03:46:18 2000



From: Alexander Mironov :      mironov-at-cmns.mnegri.it
Date: Thu, 9 Mar 2000 10:40:13 +0100 (MET)
Subject: Re: methods of embedding cells cultured on glass coverslips

Contents Retrieved from Microscopy Listserver Archives
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Dear Jaci,
The problem of cell embedding after their cutivation on plastic or galss
is rather simple. You do not need to use Thermanox. You can use glass. The
trick is that immediately after polymerization you have place glasses at
-20 degree C and your samples will be detached. If thsi scheme does not
work you can use liquid nitrogen. However, do not insert smplaes into it
(only glass). If this scheme does not work you can use commercial solution
of HF (acid). It dissolve glass efficiently. For instance, coverslips are
dissolved in 30 min. Then you have to wash samples in buffer (pH7.4) for a
long time and water (several hours) otherwise you will have problems with
contrasters.
We usually glue samples after detachment from glass and work with
this sandwich. For this you have to use incomplete polymerization of Epon
on chamber slides (12 hours). Then samples can be detached at -20 and then
glued with the fresh resin for 24 hours. If you use full polymerization
time on glass or any polymerization on plastic you will not be able to
glue samples.

Sincerely yours, Alexander Mironov
Italy


On Wed, 8 Mar 2000, Jaci Lett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I know this has been covered periodically, but as I've not needed the
} technique, I've not paid attention.  I need some methods of embedding
} cells cultured on glass coverslips.  Obviously, the most important
} issue is removing the coverslip from the polymerized block.I've looked
} through my own reference materials and the only method I found involved
} using Thermanox coverslips (upon which these particular cells will not
} grow, I'm informed).Please reply dircectly to my email address as well as
} to the Listserver (I haven't received postings for several days). Thank
} you,Jaclynn M. Lett, Research Assistant    
} jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
} DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
} MO  63110voice:  314-977-0257     fax: 
} 314-977-0030
}
}
}
}



From daemon Thu Mar 09 06:45:51 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 9 Mar 2000 21:13:19 +1000
Subject: RE: methods of embedding cells cultured on glass coverslips

Contents Retrieved from Microscopy Listserver Archives
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A few moments in liquid nitrogen and the coverslip will come off.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 09, 2000 10:10 AM, Jaci Lett [SMTP:jmlett-at-cid.wustl.edu]
wrote:
}
}
} I know this has been covered periodically, but as I've not needed the
} technique, I've not paid attention.  I need some methods of embedding
} cells cultured on glass coverslips.  Obviously, the most important
} issue is removing the coverslip from the polymerized block.I've looked
} through my own reference materials and the only method I found involved
} using Thermanox coverslips (upon which these particular cells will not
} grow, I'm informed).Please reply dircectly to my email address as well as
} to the Listserver (I haven't received postings for several days). Thank
} you,Jaclynn M. Lett, Research Assistant    
} jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
} DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
} MO  63110voice:  314-977-0257     fax: 
} 314-977-0030
}
}



From daemon Thu Mar 09 06:45:51 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 9 Mar 2000 22:00:42 +1000
Subject: RE: 2.5% glut shelflife

Contents Retrieved from Microscopy Listserver Archives
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I presume all microscopists use somewhat arbitrary shelf-life assumptions,
because we know from experience that within those constraints there normally is
no trouble. UA in water } 1 month, made up Spurr's in freezer } 3 months, Lead
Citrate } 1 year. For working strength GA I used quite conservatively 1 week.

GA forms an undesirable polymer with time and temperature. So freezing the bulk
stock (ampoules can stand a fridge-freezer) results in a very long life,
certainly a couple of years and just refrigerated its several months. This is
arbitrary too since there is no definite cut-off when the stuff suddenly
becomes useless.

I saw a publication many years ago that claimed the "undesirable polymer"
protected cells against osmotic shock and in structural studies some polymer
may be desirable (the amount can be determined simply in a spectrometer, please
don't ask for the peak locations. I don't carry that info!) Apparently its
different for immunological studies, for that the freshest GA should be used.

Soon after Sabatini advocated GA for TEM use, I guess now over 30 years ago, as
noted, some studies related polymer to storage time/temperature. I don't think
that these looked at working strength solutions, but I expect that there would
be no difference in relative polymer concentrations.

The reason for shorter shelf-life for working solutions was assumed and
probably originated with the common use of additives like sucrose or phosphate
buffers. Sucrose in an Os fixatives leads to obvious oxidation within hours. I
doubt that cacodylate in GA would be a problem and expect that it would remain
useable for several months when refrigerated. I have never bothered to test
this, however, and stuck with an assumed short shelf-life for working strengths
GA.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 09, 2000 10:09 AM, Schmitz, Robert [SMTP:rschmitz-at-uwsp.edu]
wrote:
}
}
} Below is a question from one of my former students who is now working in a
} hopsital histotech lab. It concern the selflife of glutaraldehyde fixative.
} I always mix my fix fresh, so some other perspectives might be useful!!
} Here's the Question:
}
} } My boss wants to know how long a working solution of glutaraldehyde
} } remains stable (2.5% in cacodyalate buffer). How long can we keep it in
} } the refrigerator?
} }
} Bob
} Robert J. Schmitz
} Electron Microscope Lab
} Department of Biology, CNR Building
} University of Wisconsin, Stevens Point
} Stevens Point, WI 54481
} phone (715) 346-2420
} FAX (715)346-3624
} email rschmitz-at-uwsp.edu
} http://biology.uwsp.edu/faculty/RSchmitz/home.html
}
}



From daemon Thu Mar 09 08:19:13 2000



From: puusimak-at-paju.oulu.fi
Date: Thu, 9 Mar 2000 08:06:45 -0600
Subject: fixation-method needed

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Email: puusimak-at-paju.oulu.fi
Name: Päivi Maria Susanna Uusimäki

School: the university of Oulu

Question: What is a suitable fixation-method for my
specimens? I am trying to find antigen-antibody
interactions on the bacterial membrane. My
bacteria are different strains of LABs, my special
interests are lipoteichoic acids and I am using
confocal and fluorescence microscopes. The big
problem has
been : specimens tend to "float away" under was-
hings !




From daemon Thu Mar 09 14:30:51 2000



From: Chris Adams :      cadams-at-lanl.gov
Date: Thu, 9 Mar 2000 08:19:26 -0700
Subject: TEM: Electron Tomography

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I have recently been asked to participate in an effort at Los Alamos
concerned with Electron Tomography of polymers. I haven't done the
required image reconstruction before. Does anyone know of any
commercially available software that will reconstruct several 2D
images taken at various tilt angles into a single 3D reconstruction
of an object? I suppose it would be analogous to the software used
for CAT scans? Also, if you've done this before, what kind of
hardware requirements are there? I've got a JEOL 2010 configured at
the moment to allow only ±30 degrees of tilt. Is that enough? I
assume I also need some way of automating the specimen tilting
process and will need beam blanking to avoid specimen damage. Can
anyone confirm or deny all this?

Thanks in advance for your help,

Chris



From daemon Thu Mar 09 14:30:51 2000



From: Catherine Ripley :      ripley-at-aecom.yu.edu
Date: Thu, 09 Mar 2000 10:26:55 -0500
Subject: Confocal listserver

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Thanks all.
It was just some anomaly. It went through the second time.


Catherine Ripley
*****************************************
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461
*****************************************
*****************************************



From daemon Thu Mar 09 14:30:52 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 9 Mar 2000 16:51:12 +0100
Subject: Conference Announcement: EuroFE 2000

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Dear all,

EuroFE 2000 is a forum to bring together interested parties in the field of
Field Emission Technologies. This includes microscopists working with
materials used for field emission sources (DLC, Nanotubes etc.) and those
developing field emission based sources for SEM & TEM.

There is also a strong emphasis on links with other display technologies
such as LCD's, Light Emitting Polymers and Phosphors.

The conference and workshop sessions will build on the success of EuroFE '99
& will be held in the UNESCO World Heritage city of Segovia.

The aim of this conference will be to focus on the European dimension of
Field Emission, on Industrial Problems and to bring together in a Forum the
various groups (from Industry and Public Institutions) working in this area.
In addition to the normal program of keynote speakers, oral presentations
and poster sessions, it is also planned to hold workshops on topics such as
"overcoming obstacles to industrial production".

In common with EUROFE'99 conference, there will be an emphasis on the
applications of field emission technologies, as well as basic scientific
research. A crucial issue during a conference is time allowed to discussions
between participants in order to exchange ideas and therefore possibly
define strategies in order to solve real problems. During EUROFE'2000,
following each session, large breaks will be set-up in order to allow these
discussions.

One of the major sessions will be related to Field Emission applications
such as future big FE flat panel displays (1m2). Companies such as Motorola,
Samsung, PixTech, Candescent and Saint-Gobain will participate actively in
this session. The "BigFED" project coordinated by EUROFE will be presented
at the conference in order to define strategies to be able to present
projects to the EU within this objective.

The key topics of interest will be:


Industrial applications (Flat panel displays, etc.) and related
problems (reliability, lifetime, etc.)
Field Emission from diamond, DLC and nanotubes
FE simulation and modelling
Understanding Field Emission
Space applications
Device characterisation (surface analysis, etc.)
Novel cathodes - technology and fundamentals
Other related areas: (Phosphors, LCDs, OLEDs, CRTs, New materials,
Novel devices, etc.).

An important issue during conferences is to make possible student
participation in order to form them and allow initiation of contacts with
either industry or public institutions. For this reason, this year, the
EUROFE2000 organisation set-up a special reduced registration fee for
students including also the accommodation.


Regards

Tim


******************************************************************
Tim E. Harper EuroFE Network Co-Chairman
EuroFE is a network of the European Science Foundation
Phone +34 91 640 71 85 Fax +34 91 640 71 86





From daemon Thu Mar 09 14:30:52 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 9 Mar 2000 09:56:57 -0600 (CST)
Subject: Re: Rapid Fixation of cells for SEM)

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Responding to the message of {v03007805b4ec9d6d6eae-at-[206.69.208.21]}
from R schlicher {rkslick-at-yahoo.com} :

} I need to fix cells for viewing with SEM. However,
} the fixation method needs to be consistent with my
} experimental methods:
}
} 1) I need to fix the cells in less than 1 minute and
} due to this time limitation, I cannot spin the cells
} into a pellet and must add the cell suspension to the
} fixative; and

snip!

I would like to try chemical
} fixation and SEM. It has been suggested that I try
} glutaraldehyde + buffers, then postfix with osmium
} tetroxide and dehydrate in an ethanol series.

Robyn,

You could try simultaneous glutaraldehyde + osmium fixation, which should work
well for cells in suspension in particular. Mix up seperate solutions of each,
such that when mixed in equal amounts, you get the concentrations of fixes and
buffers you want. Its usually recommended that you do this at lower
temperatures, like about 4C, so pre-cool the solutions and vials first, to
prevent the glut and osmium from fighting with each other, but even for "less
than a minute", as you put it, maybe you could still work at room temperature.
Try mixing the two fixatives together without cells first, to determine the
maximum time until discoloration occurs, which means the osmium is precipitating
out or whatever goes on there.

Your first rinse could just be a large dilution, to seperate the warring
fixatives, then spin down and rinse a few more times before going into the
dehydration series.

Give me a few hours to dig out references for this technique, will send them out
later.

Gib






Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Mar 09 14:30:53 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 9 Mar 2000 10:44:28 -0600 (CST)
Subject: Re: Rapid Fixation of cells for SEM)

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Robyn,

Here are two references for the simultaneous glut/osmium technique I mentioned
earlier.


1. Franke, W.W., Krien, S. & Brown, J.R.M. (1969) Simultaneous glutaraldehyde
osmium tetroxide fixation with post osmication. Histochemie, 19, 162-164.

2. Hirsch, J.G. & Fedorko, M.E. (1968) Ultrastructure of human leukocytes after
simultaneous fixation with glutaraldehyde and osmium tetroxide and post fixation
in uranyl acetate. J. Cell Biol. 38, 615-627.


Good luck!

Gib Ahlstrand


Responding to the message of {v03007805b4ec9d6d6eae-at-[206.69.208.21]}
from R schlicher {rkslick-at-yahoo.com} :

} Hello,
}
} I need to fix cells for viewing with SEM. However,
} the fixation method needs to be consistent with my
} experimental methods:
}
} 1) I need to fix the cells in less than 1 minute and
} due to this time limitation, I cannot spin the cells
} into a pellet and must add the cell suspension to the
} fixative; and
}
} 2)I am looking for "effects" to the cell membrane
} caused by my experiment, so I need to keep any
} possible fixative damage/effects at a minimum.
}
} I have fixed cells using plunge freezing and viewed
} them with TEM, this is time consuming and I ran into
} a
} lot of freeze damage. I would like to try chemical
} fixation and SEM. It has been suggested that I try
} glutaraldehyde + buffers, then postfix with osmium
} tetroxide and dehydrate in an ethanol series.
}
} Is this the best method? I am open to suggestions.
} I am using suspensions of prostate cancer cells in
} RPMI (+serum) growth media; I can perform the
} experiment in buffered saline and use a high
} concentration of cells.
}
} Thanks,
} Robyn
}
}
} Robyn K. Schlicher, M.S.
} Laboratory for Drug Delivery
} Institute for Bioengineering and Biosciences
} Georgia Institute of Technology
} 315 Ferst Drive
} Atlanta, GA 30332
} rkslick-at-yahoo.com


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Mar 09 14:30:55 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 9 Mar 2000 13:44:11 -0400
Subject: Re: methods of embedding cells cultured on glass coverslips

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At 6:09 PM -0600 3/8/0, Jaci Lett wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Your are left with a mirror-finish block face that is perfectly intact.
Even with all the safety precautions, I find this method preferable and
more reliable in my hands than the "heat and snap-off" method that many
people use.

Just don't let your students do it!

Good luck,
Lee



Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Thu Mar 09 14:30:57 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 09 Mar 2000 10:04:34 -0800
Subject: immunofluorescence in methacrylate

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Dear microscopists,
Can anyone offer advice on doing immunofluorescence at the light level? I
have cryopreserved plant tissue that I am contemplating embedding and using
for immunolocalization. I have a protocol that uses butyl, methyl
methacrylate. I am wondering several things. Why butyl, methyl
methacrylate? Are there other resins/media that are sufficient? I'd
appreciate any advice anyone has.
Kristen Lennon
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Thu Mar 09 14:30:57 2000



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Thu, 09 Mar 2000 10:20:15 -0500
Subject: Mat: Elemental Boron Manufacturers

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I have a question that is not a typical microscopy question but I thought I
would call upon the extensive knowledge of the readers of this list server.

Does anyone know who manufacturers elemental boron?

We are using boron as a substrate for particle microanalysis (especially
carbonaceous particles). Elemental boron can be purchased in the form of
“lumps” or “nuggets” several centimeters in dimension from chemical supply
houses such as Alfa Aesar and Aldrich. These companies however will not
reveal their suppliers and so any questions that I want to ask the
manufacturer have to go through the third party supply house. In addition
to being silly, this procedure is extremely slow and inefficient. I would
prefer to bypass the third party altogether.

So if anyone has any idea who manufacturers elemental boron I would greatly
appreciate hearing from you.

Disclaimer: Any opinions expressed are my own and not those of my
employer, The Federal Government.


Eric Windsor

Eric S. Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
Fax: (301) 417-1321
Eric.Windsor-at-NIST.Gov



From daemon Thu Mar 09 14:30:58 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Thu, 9 Mar 2000 13:08:37 -0500 (EST)
Subject: TEM, bio, Ag/Au enhancement

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Greetings!

One of the researchers here is trying to do silver and/or gold enhancement
of an immunolabel in brain slices, but is getting amazing background. We
switched to the gold enhancement because everyone was saying how much
cleaner it is....didn't help. I've seen one report in which the
researchers pre-washed their samples in EDTA, because they felt that they
were getting background from Mg in the tissue that was nucleating silver
deposition - anyone else have any thoughts/experience on this? Anyone else
doing enhancement in tissue slices?

He is using kits to do the enhancement - I'm not sure which companies are
the suppliers, but they aren't supposed to be light-sensitive (my first
culprit). The samples are washed extensively in water before enhancement,
and even the no-antibody controls are "lighting up".

Any suggestions will be greatly appreciated!

Tamara Howard
CSHL



From daemon Thu Mar 09 14:31:00 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Thu, 09 Mar 2000 13:49:03 -0500
Subject: Re: TEM: Electron Tomography

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Chris,

I do not have the information you need but I am old enough to remember
seeing one of the first papers on tomography. It chose a cute title,
something like:
"Algorithm for Reconstructive Tomography or ART"
Which was fine until the next issue of the journal had a paper (giving an
improvement on the method) called:
"Fast Algorithm for Reconstructive Tomography."

Good luck,
Alwyn Eades



From daemon Thu Mar 09 14:31:03 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 9 Mar 2000 11:58:54 -0700
Subject: RE: Transmission electron microscopy

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Peter,

a transmission electron microscope is in principle the same as a
transmission light microscope, except that it uses high energy electrons
instead of light. This allows a much higher resolution (it is possible
to see atoms or cyrstalline structures), but necessitates much bigger
instruments, as there is vacuum technology, high voltages and X-ray
generation involved. Many different techniques are available on these
machines.

Rather than explaining all to you, you could do a search on the web
looking for "transmission electron microscopy" or "TEM". Another
starting point would be to go to

http://ncmi.bcm.tmc.edu/

and select one of the sites under "Links". Or go to the MSA site

http://www.msa.microscopy.com/

and find your way from there. I am sure people will help you here if you
have specific questions.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From: JUPE Peter[SMTP:JUPE.PETER-at-HDH.COM.AU]
} Sent: Monday, March 06, 2000 8:35:46 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Transmission electron microsocopy
} Auto forwarded by a Rule
}
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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Sir
Could you please supply me details on "transmission electron
microsocopy"
for my 16yr old daughter at high school, or alternatively where to look
on
the Internet.
Thanking you
Peter Jupe



From daemon Thu Mar 09 14:31:03 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Thu, 09 Mar 2000 11:27:43 -0800
Subject: Re: methods of embedding cells cultured on glass coverslips

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Dear Jaci. If you wish to have access to all the cells on the coverslip,
the best way is to flat embed the whole coverslip in a Chang monolayer mold
(20mm or 10mm) available from EMS. Be sure to drain the coverslips well
and wipe excess resin from the bottom of the coverslip. Invert over filled
mold and polymerize overnight. Remove from oven and use metal file to file
down edges of embedded coverslip, then immerse in concentrated hydrofluoric
acid (use plastic forceps, work in fume hood, wear nitrile glove sand rinse
everything well with water). It will dissolve the glass in 15-30 mins. It
works every time and you have a complete embedded coverslip. You can then
stain it with toludine blue, find the identified cell, mark it with a Ladd
marking objective. I then punch out the cell(s) of interest using a hole
punch and attach the 5mm circles to blank stubs using quick setting
epoxi-patch bond (hardens in 5-10 minutes). This is available from Dexter
Corporation 1-603-474-5545 and works extremely well. I sometimes have low
contrast so Alexander Mironov's washing method may solve this problem.
Otherwise, stain for 30 mins in 5% aqueous UA and 3 mins in Lead citrate
for good results. Good luck, JoAnn Buchanan



From daemon Thu Mar 09 14:31:05 2000



From: hadden-at-wingate.edu (Lee Hadden)
Date: Thu, 09 Mar 2000 14:51:27 -0500
Subject: suggestions for new SEM??

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When I stepped outside of the science building tonight, I ran into,
literally, our university president who said he was just walking over to
try to find me. He cautioned me about overreacting and then proceeded to
tell me to go ahead and get my best quote on a new SEM and service
contract!!!!!!

Now that I've got both feet back on the ground, I need any help that any
of you can provide, based on your experience and knowledge. We're
looking for a new SEM that will support undergraduate courses, be a good
training machine for students, support student and faculty research and
have a good track record for reliability and being reasonably user
friendly. It should be state of the art, but not be so expensive to
operate and keep up that it would limit how much students and faculty
could use it.

I was trained on an Amray in the 80's and ran our TEM until it became
cost ineffective a few years ago. But I know things have changed
considerably since my pre-computer controlled everything SEM's and would
appreciated the benefit of any experience any of you might have had with
new SEMS.

Anything to recommend? Models? Features to insist on or avoid?
Reliability, service costs and frequency? Companies to go with or stay
away from? What new models do you have and would you recommend them to
others? Why or why not?

I'd really appreciate any and all insight you can provide. When your
president walks to YOUR office and says the equipment grant proposal HE
made to a major foundation was just approved and that he needs me to get
the best quote on an SEM to him ASAP, I want to have something to go on,
and reasons to go along with it based on user input, without delay.

Thanks in advance for any assistance you can provide.

Lee Hadden
Professor and Chair,
Department of Biology
Wingate University
Wingate, NC 28174

hadden-at-wingate.edu
http://www.wingate.edu
704-233-8238




From daemon Thu Mar 09 18:37:32 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Thu, 9 Mar 2000 20:28:12 +0100
Subject: UV spectra of Glutaraldehyde

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I am only using distilled Glutaraldehyde - and only up to 7 days
after making up the fixative (Karnovsky's) and storing it in the fridge.

UV Spectra of pure Glutaraldehyde show a single absorbance peak
at 280 nm (monomeric ) - whereas the commercial material shows
an additional stronger peak at 235nm. This is assumed to be due
to the formation of oligomer and polymer.
The ratio of absorbance at 235 and 280 nm can be used as a
measure of the quality of the GA.

Monomeric-polymeric mixtures yield good ultrastructural
preservation when the ratio of monomeric to polymeric forms is 1:1
or 1:2. Ratios outside this limits may prove unsatisfactory.
(Weakley 1974)
(unfortunately I do not have the exact source/ Paper handy where I
got that from.)


Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)181 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Thu Mar 09 18:37:33 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 9 Mar 2000 14:41:30 -0600
Subject: glass coverslip detachment

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I have 95% success detaching glass coverslips embedded with epon inverted on beem capsules described by Leona by simply placing the polymerized coverslip on a hotplate ( } 85.C) with the beem capsule up and gently prying them apart with a straigtedge razor. Cells stay where you want them. I found by that instead of using the beem capsules I just use the lid of one or the lid of a microfuge tube ( makes a better platform). Once separated put the top on a slide and the cells are easily visualized with brightfield (osmicated) or phase (non osmicated).
Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX



From daemon Thu Mar 09 18:37:36 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 09 Mar 00 12:57:58 -0800
Subject: Re: methods of embedding cells cultured on glass coverslips

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Reply to: Re: methods of embedding cells cultured on glass coverslips
Growing cells on coverslips, fixing them in situ and processing them into epoxy resin is fairly straight forward. Remember that the cells are a monolayer and are only about 30 microns thick so fixation, rinsing, dehydration and infiltration times can be cut down considerably.

The final step of removing the coverslip from the polymerized block is not, however, as trivial as everyone makes it out to be. Yes, dipping the warm block in liquid nitrogen and then re-warming it, will eventually take the glass off the plastic (leaving the cells in the block), but only if the thin layer of plastic that always seems to appear on the back of the glass is removed.
The block and glass are also more easily removed if the resin is polymerized with the glass resting cell-side down on resin. The alternative way, of immersing the coverslip cell-side up, and letting the resin cover the cells, is safer, but leads to problems in removing the glass.

If there is a problem with removing the glass, making scratches with a diamond scribe can help and sometimes bending the whole block and glass coverslip can be useful ( if this is possible).

Don't worry about immersing the block in the nitrogen either, there really doesn't seem to be just one way that this happens. If the process is able to remove the glass, the glass may shoot off so violently you may wish you had put on your safety glasses. The next block may not work.

One other method I heard of was to put the block, glass-side down, on a hot plate. Wait until the glass gets hot and I'm told it will easily slide off. I never tried it.
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Alexander Mironov wrote:
}
} Dear Jaci,
} The problem of cell embedding after their cutivation on plastic or galss
} is rather simple. You do not need to use Thermanox. You can use glass. The
} trick is that immediately after polymerization you have place glasses at } -20 degree C and your samples will be detached. If thsi scheme does not
} work you can use liquid nitrogen. However, do not insert smplaes into it
} (only glass). If this scheme does not work you can use commercial solution
} of HF (acid). It dissolve glass efficiently. For instance, coverslips are
} dissolved in 30 min. Then you have to wash samples in buffer (pH7.4) for a
} long time and water (several hours) otherwise you will have problems with
} contrasters. } We usually glue samples after detachment from glass and work with
} this sandwich. For this you have to use incomplete polymerization of Epon
} on chamber slides (12 hours). Then samples can be detached at -20 and then
} glued with the fresh resin for 24 hours. If you use full polymerization
} time on glass or any polymerization on plastic you will not be able to
} glue samples.
}
} Sincerely yours, Alexander Mironov
} Italy
}
}
} On Wed, 8 Mar 2000, Jaci Lett wrote:
}
} } I know this has been covered periodically, but as I've not needed the
} } technique, I've not paid attention.  I need some methods of embedding
} } cells cultured on glass coverslips.  Obviously, the most important
} } issue is removing the coverslip from the polymerized block.I've looked
} } through my own reference materials and the only method I found involved
} } using Thermanox coverslips (upon which these particular cells will not
} } grow, I'm informed).Please reply dircectly to my email address as well as
} } to the Listserver (I haven't received postings for several days). Thank
} } you,Jaclynn M. Lett, Research Assistant    
} } jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
} } DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
} } MO  63110voice:  314-977-0257     fax: 
} } 314-977-0030
} }

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Mar 09 18:37:39 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 09 Mar 2000 16:02:21 -0500
Subject: Re: TEM: Electron Tomography

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Chris Adams wrote:

Dear Chris,


} I have recently been asked to participate in an effort at Los Alamos
} concerned with Electron Tomography of polymers. I haven't done the
} required image reconstruction before. Does anyone know of any
} commercially available software that will reconstruct several 2D
} images taken at various tilt angles into a single 3D reconstruction
} of an object?

Yes. SPIDER is commercially available, and will do 3D
reconstructions by any of several methods. Check it out at
www.wadsworth.org; click on Resources (pretty far down on the
left-hand side); then click on SPIDER.


} I suppose it would be analogous to the software used
} for CAT scans?

Some of the reconstruction methods, at least, are used in
CAT scans, but others (e.g., projection onto convex sets) may not
be--I don't know the latest software for CAT scans.


} Also, if you've done this before, what kind of
} hardware requirements are there?

We run on an Onyx using unix; I don't know all the available
versions of SPIDER.


} I've got a JEOL 2010 configured at
} the moment to allow only ±30 degrees of tilt. Is that enough?

No. +/- 50 deg is adequate, +/- 60 deg is better.


} I assume I also need some way of automating the specimen tilting
} process and will need beam blanking to avoid specimen damage. Can
} anyone confirm or deny all this?
}

Automation is optional, but beam blanking is mandatory--
especially for cryo-specimens. If your goniometer is sufficiently
accurate, you can change the tilt setting with the beam blanked,
then adjust the position and focus quickly after the beam is back
on. If you don't have a sufficiently good goniometer, you need
to have either a low-dose kit (assuming you can recognize both
your area of interest and a nearby area for focusing) or a very
sensitive video-rate camera, such as the intensified CCD we have
on the HVEM, so you can find and focus the area of interest with
minimal exposure of the specimen.


}
} Thanks in advance for your help,
}

You're welcome.
Yours,
Bill Tivol



From daemon Thu Mar 09 18:37:43 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 9 Mar 2000 16:41:52 -0600
Subject: Re: immunofluorescence in methacrylate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Kristen,
I have published several papers on using
butylmethylmethacrylate for immuno at the light level, including a
paper where cryofixation and freeze substituion were used, on plant
material. Perhaps the protocol you have is by me?

To answer your questions, a mixture of butyl and methyl
methacrylate gives nice blocks and preserves the tissue well. I have
tried using pure butyl or pure methyl and the sectioning or
preservation was worse. These resins, alone or in combination, have
the crucial advtange of being mostly removable post embedment with a
brief incubation in actetone. This improves the access of your
antibody to your antigen greatly. It is important when doing immuno
work at the light level on sections to remember that if an antibody
can penetrate say 15 nm into the plastic (I am guessing at this
number), this is most of the thickness of an ultra thin section but
only a teeeny bit of a semithin section. So, for light work
especially, being able to remove the embedment is crucial. The usual
way to do this is to embed in paraffin and this is fine if you just
want tissue level distinctions. But if you want subcellular
localization or for other reasons you want your stuff to be better
preserved, then plastic gives much nicer results.
DOes this help?
I will be quite happy to help you further with butylmethyl
methacrylate, as I have a professional fondness for the stuff.
Good luck,
Tobias

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Thu Mar 09 18:37:45 2000



From: DDittus787-at-aol.com
Date: Thu, 9 Mar 2000 17:56:03 EST
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
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Dear Tamara
Is he washing in de-ionized water? Brain is always absorbent, so timing and
reagent volumes may need adjustment. Gold and silver enhancement are tricky
so I would protect from heat and light as much as possible. Good Luck.
Dana



From daemon Thu Mar 09 18:37:47 2000



From: AMaterEval-at-aol.com
Date: Thu, 9 Mar 2000 18:24:42 EST
Subject: TOPSCAN Confocal Microscope Availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for someone to provide analytical services on analyzing
surface topography on biomedical implants specifically using a TOPSCAN
confocal microscope. It needs to be performed on this instrument for
comparison with data previously taken on such an instrument. Does anyone
know of anyone who has this instrument and who might entertain performing
such an analysis? Your help on this will be greatly appreciated.

Charles R. Anderson, Ph.D.
President
Anderson Materials Evaluation, Inc.
1450 South Rolling Road
Halethorpe, MD 21227
(410) 455-5698
Fax (410) 455-5679

An independent materials analysis laboratory for failure analysis, quality
control, product and process development. We offer small-spot XPS, Auger
microprobe, AFM, SEM, optical metallographic microscopy, white light
interference microscopy, mass spectroscopy, and thermal analysis (TGA, DSC,
TMA, DMA) services.



From daemon Thu Mar 09 18:37:50 2000



From: PESTOEM-at-aol.com
Date: Thu, 9 Mar 2000 18:50:23 EST
Subject: Objective Aperture Moving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To All:
We have a Jeol 100CX II and experiencing a constant moving of the Objective
Aperture moving when scanning grids, especially in Posistion # 2 Grid.
Any solution or ideas? We changed apertures,cleaned holder etc.
Thank you,
Peter Stolzenberg, PESTO INC.
pestoem-at-aol.com



From daemon Thu Mar 09 18:37:51 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 9 Mar 2000 19:00:13 -0500
Subject: RE: suggestions for new SEM??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just bought a new top-end type microscope (Field emission semi-inlens
SEM). I evaluated four companies. I would have been happy with any of the
microscopes that I looked at. In my opinion, all of the microscopes
available today are damn good throughout the product range. You will
probably be happy with any microscope that you buy. If your budget is
limited and defined as ours was, that is going to limit you as to what
machine that you will buy. You can use that to see what the manufacturers
will put into your package. There are other intangibles that go into the
decision. The most important is service, in particular, the service in your
area. Ask around! I did and got some interesting comments. Then there are
some of the others that you have to judge for yourself. Things like the
user interface -you will be training students to use it, you want a
versatile machine, but one that is easy to learn to use; output
capabilities; digital/film or both; networkability; etc.

If performance is the issue, then the best thing that you can do is to take
a number of your samples and take them to the application labs and run them
while you are there. You should take samples that are representative of the
types of samples that you will be working with. Make sure that you compare
the same conditions on all your samples and that the output that you get
back can be compared at the same magnifications. Standardize the output
that you want the images to be in, for example 8 bit grayscale TIFF images
or whatever. You should also do the samples in the same order from lab to
lab. Print the results from digital images on the same printer. Then get a
group of experienced users to help you judge the results.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."

--

} -----Original Message-----
} From: hadden-at-wingate.edu [ mailto:hadden-at-wingate.edu
{mailto:hadden-at-wingate.edu} ]
} Sent: Thursday, March 09, 2000 2:51 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: suggestions for new SEM??
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
} --------------------------------------------------------------
} ---------.
}
}
} When I stepped outside of the science building tonight, I ran into,
} literally, our university president who said he was just
} walking over to
} try to find me. He cautioned me about overreacting and then
} proceeded to
} tell me to go ahead and get my best quote on a new SEM and service
} contract!!!!!!
}
} Now that I've got both feet back on the ground, I need any
} help that any
} of you can provide, based on your experience and knowledge. We're
} looking for a new SEM that will support undergraduate
} courses, be a good
} training machine for students, support student and faculty
} research and
} have a good track record for reliability and being reasonably user
} friendly. It should be state of the art, but not be so expensive to
} operate and keep up that it would limit how much students and faculty
} could use it.
}
} I was trained on an Amray in the 80's and ran our TEM until it became
} cost ineffective a few years ago. But I know things have changed
} considerably since my pre-computer controlled everything
} SEM's and would
} appreciated the benefit of any experience any of you might
} have had with
} new SEMS.
}
} Anything to recommend? Models? Features to insist on or avoid?
} Reliability, service costs and frequency? Companies to go
} with or stay
} away from? What new models do you have and would you
} recommend them to
} others? Why or why not?
}
} I'd really appreciate any and all insight you can provide. When your
} president walks to YOUR office and says the equipment grant
} proposal HE
} made to a major foundation was just approved and that he
} needs me to get
} the best quote on an SEM to him ASAP, I want to have
} something to go on,
} and reasons to go along with it based on user input, without delay.
}
} Thanks in advance for any assistance you can provide.
}
} Lee Hadden
} Professor and Chair,
} Department of Biology
} Wingate University
} Wingate, NC 28174
}
} hadden-at-wingate.edu
} http://www.wingate.edu {http://www.wingate.edu}
} 704-233-8238
}
}
}



From daemon Thu Mar 09 18:37:57 2000



From: Hong Yi :      hyi-at-emory.edu
Date: Thu, 9 Mar 2000 19:29:00 -0500 (EST)
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tamara:
To figure out what went wrong with your investigator's
experiment, we need more detail about how his experiment was conducted.
Would you ask him to contact me and email his protocol to me? I believe
that I can help him.

Hong
==============
Hong Yi
Emory Neurology
hyi-at-emory.edu

On Thu, 9 Mar 2000, Tamara Howard wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings!
}
} One of the researchers here is trying to do silver and/or gold enhancement
} of an immunolabel in brain slices, but is getting amazing background. We
} switched to the gold enhancement because everyone was saying how much
} cleaner it is....didn't help. I've seen one report in which the
} researchers pre-washed their samples in EDTA, because they felt that they
} were getting background from Mg in the tissue that was nucleating silver
} deposition - anyone else have any thoughts/experience on this? Anyone else
} doing enhancement in tissue slices?
}
} He is using kits to do the enhancement - I'm not sure which companies are
} the suppliers, but they aren't supposed to be light-sensitive (my first
} culprit). The samples are washed extensively in water before enhancement,
} and even the no-antibody controls are "lighting up".
}
} Any suggestions will be greatly appreciated!
}
} Tamara Howard
} CSHL
}
}





From daemon Fri Mar 10 07:17:23 2000



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Fri, 10 Mar 2000 15:30:46 +1300
Subject: Hitachi H-600 Parts Needed

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a scrapped CRT (with circuit board) for data display that
they can part with? Ours has given up finally.....

Also, I was wondering if there were any hot or cold stages still out there
for the old H-600.

We are happy to pay for these things if they turn up somewhere.

Thanks
Milo Kral
University of Canterbury
Department of Mechanical Engineering
Christchurch
New Zealand




From daemon Fri Mar 10 07:17:31 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 9 Mar 2000 23:22:58 -0500
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Tamara:

The topic of reducing background has been raised a number of times by our
customers, and we have collected together several approaches and their
references, and I hope some of the following are helpful:

One possible mechanism for "background" signal is hydrophobic interactions
between gold particles and tissue components, and if your samples can
tolerate them, additives which break these interactions down and solubilize
hydrophobic species might help in reducing background. Washes which
effectively solubilize gold compounds and other hydrophobic species
include:

* 0.6 M triethylammonium bicarbonate buffer (prepared by bubbling
CO2 into an aqueous suspension of triethylamine with stirring;
for a reference, see Safer, D.; Bolinger, L., and Leigh, J. S.;
J. Inorg. Biochem., 26, 77 (1986)).

* A small amount of detergent, such as Tween-20 or Triton X-100,
or an amphiphile such as benzamidine or 1,2,3-trihydroxyheptane.

* If you are using the gold enhancer, raising the ionic strength
of the solution is sometimes helpful - we have found that
raising the sodium chloride concentration in the actual gold
enhancement mixture to 0.5 M can actually reduce the background.
Alternatively, lowering the pH of the gold enhancement mixture
by about 0.5 pH units may help.

Acetonitrile coordinates quite well to silver ions, so if your background
problem is due to the interaction of silver from the enhancement reagents
with a component of your tissues, treatment with acetonitrile or a wash
solution containing a proportion of acetonitrile may help (provided the
sample can withstand it).

To reduce the background for silver enhancement, one procedure which has
been found to be effective is to wash with 0.02 M sodium citrate buffer, pH
7.0, several times immediately before silver enhancement - this has been
used to reduce background in experiments with the combined fluorescein and
gold probe FluoroNanogold when used with HQ Silver (Nanoprobes). When the
Danscher formulation of silver enhancer was used instead, 0.02 M sodium
citrate buffer at pH 3.5 was found to be most effective. In immunoblots, we
have also observed that washing with 0.05 M disodium EDTA, pH 4.6, before
silver enhancement also results in low background. (Reference: Powell, R.
D.; Halsey, Carol M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and
Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous"
detection of a pre-mRNA splicing factor by light and electron microscopy.
J. Histochem. Cytochem., 45, 947-956 (1997)).

A number of methods have been described for stopping the silver enhancement
reaction or for "back-developing" to remove extraneous deposited silver.
These prevent the continuation of the reaction in the specimens after
development is complete (for example, if the silver is only slowly removed
from the tissue), and may help reduce your background signal.

Sodium thiosulfate (1 % aqueous solution, freshly made) is a good "stop"
reagent for both silver and gold deposition (Van Driel, D. 1997. Gold
toning for silver enhanced immunogold reacted tissue. Micros. Today, 97-7,
28), so it is reasonably safe to assume that there will be no further
deposition after it is applied. However, one caveat: you should use caution
with gold enhancement because there have been reports that sodium
thiosulfate can remove the enlarged gold particles. This might be a good
choice to begin with (1-2 minute incubation after enhancement and washing
with water; after the sodium thiosulfate, rinse thoroughly again with
deionized water).

Other reaction stop and back development methods include:

(i) 1% acetic acid (Scopsi, L. 1989. Silver-enhanced colloidal gold
method., p. 260. In M. A. Hayat (ed.), In Colloidal Gold: Principles,
Methods, and Applications, vol. 1. Academic Press, San Diego, CA).

(ii) 1% acetic acid followed by photographic fixer (Agefix, Agfa-Gevaert,
or Ilfospeed 200, Ilford) (Scopsi, L. 1989., same reference as (i)).

(iii) direct photo fix, using those just mentioned (Burry, R.W. 1995.
Pre-embedding immunocytochemistry with silver-enhanced small gold
particles, p. 217-230. In MA Hayat (Ed.). Immunogold silver staining:
Principles, methods and applications. CRC Press, Boca Raton).

(iv) brief rinse in 2.5% sodium chloride (Scopsi, L. 1989., same reference
as (i)).

(v) 15-25% aqueous sodium thiosulfate plus 15% sodium sulfite (Danscher, G.
1981. Histochemical demonstration of heavy metals. A revised version of the
silver sulphide method suitable for both light and electron microscopy.
Histochemistry 71, 1-16).

(vi) 1% acetic acid, washes in acetate buffer, toning in 0.05% HAuCl4 3-10
min, with excess silver removed with 3% sodium thiosulfate. We found that
Nanogold-labeled proteins run on a polyacrylamide gel kept low backgrounds
when stopped with 10% acetic acid with 10% glucose in water, as opposed to
just a water stop (Takizawa, T. and J.M. Robinson. 1994. Use of 1.4-nm
immunogold particles for immunocytochemistry on ultra-thin cryosections.
J. Histochem. Cytochem. 42, 1615-1623).

(vii) A modified Farmer's solution was used for the reversal (0.3 ml 7.5%
potassium ferricyanide, 1.2 ml of 20% sodium thiosulfate, 60 ml water)
(Danscher, G.: Histochemistry, 71, 1-16 (1981)). Application of this
solution briefly to your sample before gold toning may help to remove
background silver deposition.

Hope this helps,

Rick Powell


**********************************************************************
* - PLEASE NOTE OUR NEW ADDRESS - EFFECTIVE FEBRUARY 15, 2000 - *
* *
* NANOPROBES, Incorporated | US Toll-free: (877) 447-6266 *
* 95 Horse Block Road | Tel: (919) 510-0590 *
* Yaphank, NY 11980-9710, | Fax: (919) 510-0590 *
* USA | rpowell-at-nanoprobes.com *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************




From daemon Fri Mar 10 07:17:42 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Fri, 10 Mar 2000 02:10:24 -0500
Subject: Looking for EDS system.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers'

Please help this gentleman. He needs inexpensive EDS system suitable for
Philips TEM (horizontal entrance detector) complete, and old EDAX-9800 or
similar, computer unit only. Reply to Bill Lawry at eht-at-stlnet.com or
(314)531-9868. Thank you.


Vitaly Feingold
Scientific Instruments and Applications
(770)232-7785 ph.
(770)232-1791 fax.



From daemon Fri Mar 10 07:17:54 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 10 Mar 2000 00:43:54 -0800 (PST)
Subject: Re: Objective Aperture Moving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Peter,
I have a 7 holed, self-cleaning, gold foil aperture in my TEM. The hole
diameters are 30um and 60um. At 60kV, the aperture does tend to wander
abit until it gets warmed up. After an hour or so of operation, I
generally have to tweak the xy alignment and then I am ready to continue.

I am always amazed when the instrument is used by other investigators.
Frequently the aperture is halfway into the the beam, i.e., the crescent
of the aperture is covering 1/3 of the field of view. As I stated, I am
aware the gold foil aperture tends to drift slightly over time exposed to
the beam, and consequently adjust accordingly.

I too would be keen to hear any additional comments on this 'trait' of
gold foil apertures.

-Ken

On Thu, 9 Mar 2000 PESTOEM-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To All:
} We have a Jeol 100CX II and experiencing a constant moving of the Objective
} Aperture moving when scanning grids, especially in Posistion # 2 Grid.
} Any solution or ideas? We changed apertures,cleaned holder etc.
} Thank you,
} Peter Stolzenberg, PESTO INC.
} pestoem-at-aol.com
}
}



From daemon Fri Mar 10 07:17:55 2000



From: Carstensen, Jesper Vejl¿
Date: Fri, 10 Mar 2000 10:31:18 +0100
Subject: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I have just started on a TEM-project investigating the microstructures of
different tool steels. The steels contain various types of carbides with
different compositions, shapes and sizes. I have started out by making thin
foils using electrolytical jet polishing. I get nice thin foils where I can
study and analyse the martensitic matrix, but the carbides are off course
too thick to be analysed. To overcome this problem I have considered a
combination of electropolishing and ion milling, but I have also heard about
an extraction replication technique. However, I haven't been able to find
any literature on this technique. Have any of you tried this technique
and/or could you point out some literature describing the technique? Also,
if you have other suggestions to deal with the described problem I would be
pleased to hear about them.

Regards, Jesper

----------------------------------------------------
Jesper Vejloe Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
----------------------------------------------------





From daemon Fri Mar 10 07:17:56 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 10 Mar 2000 11:54:59 +0100
Subject: RE: Conference Announcement: EuroFE 2000

Contents Retrieved from Microscopy Listserver Archives
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Hi,

The url of the conference site seems to have been stripped out by some mail
servers. Just in case it's www.cmp-cientifica.com/eurofe

Regards

Tim




******************************************************************
Tim E. Harper EuroFE Network Co-Chairman
EuroFE is a network of the European Science Foundation
Phone +34 91 640 71 85 Fax +34 91 640 71 86





From daemon Fri Mar 10 21:18:58 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 10 Mar 2000 07:58:00 -0600
Subject: Re:suggestions for new SEM??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One accessory worth considering, especially for a SEM used by students would
be
a ChamberScope. This is a small IR video camera mounted to the chamber
which
will allow students to see the position of samples in the chamber in real
time.
Could avoid damaging crashes. Take a look at GW Electronics for one
model....

Woody White
McDermott Technology

Me:
http://www.geocities.com/capecanaveral/3722



From daemon Fri Mar 10 21:18:59 2000



From: electron microscope laboratory :      emlab-at-udsm.ac.tz
Date: Fri, 10 Mar 2000 07:24:40 -0600
Subject: training in TEM maintenance...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hallo,
could anybody on the net have an idea where one can attend a "training in
TEM maintenance".
Best regards,

Nyakyi




From daemon Fri Mar 10 21:19:01 2000



From: Bartek :      chmielow-at-immagene.mcg.edu
Date: Fri, 10 Mar 2000 09:02:53 -0500
Subject: 2-photon microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have Carl Zeiss LSM 510 2-photon microscope armed with a T:sapphire
laser. We are successful with one color imaging, but we wpould like to
try two-color two-photon imaging, and of course we have problems with
finding a proper set of fluorophoes. My understanding is that they
should have similar excitation and different emissions. Has anyone tried
using two or more fluorophores ? Which combinations are the best? What
wavelengths are recommended?

Bartek Chmielowski



From daemon Fri Mar 10 21:19:04 2000



From: Chengge Jiao :      c.g.jiao-at-bris.ac.uk
Date: Fri, 10 Mar 2000 14:35:09 -0000
Subject: Electron diffaction software

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Hi,

Does anybody know where I can get free software for electron doffraction and
more...

Can we download them free ?

Chengge JIAO

H.H.Wills Physics Lab
University of Bristol
c.g.jiao-at-bristol.ac.uk



From daemon Fri Mar 10 21:19:05 2000



From: rlvaughn-at-unmc.edu -at-sparc5.microscopy.com
Date: Tue, 7 Mar 2000 17:06:42 -0600
Subject: TEM - ImmunoEM reference

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Is there a short, general reference, like a review, that explains immuno
EM, it's uses, major techniques, and shortfalls? I would like to use it to
send to investigators that are thinking about using the technique but don't
know much about labeling at the EM level. I have a lot of specific
references and it would take a lot of time to reference or copy them. If
the investigator wants to know more I can direct them to those, otherwise a
general one would get them and me on the same page when we start talking
specifics on their project. Thanks

Rick Vaughn
rlvaughn-at-unmc.edu






From daemon Fri Mar 10 21:19:05 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 7 Mar 2000 08:48:54 -0500 (Eastern Standard Time)
Subject: Re: Microscopy of blood

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In the earlier days {25 years ago} of medical diagnosis, before the
advent of the rapid, highly specific methods of identifying pathogenic
organisms, darkfield microscopy was routinely applied to rule out
specific organisms. In particular, the diagnosis of syphilis was aided
with this mode of imaging. When fluids from a suspect lesion were
applied to a slide and imaged by darkfield, the characteristic shape of
the spirochaete organisms was clearly seen. More recently, darkfield
examination of synovial fluids from arthritic joints was used to rule
out or diagnose Lyme disease, before specific testing became available.

W. L. Steffens, Ph.D
University of Georgia





From daemon Fri Mar 10 21:19:05 2000



From: John Runions :      cjr41-at-cam.ac.uk
Date: Tue, 7 Mar 2000 11:35:10 +0100
Subject: Re: Confocal Newslist or Listserver??

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Catherine,

I also tried to subscribe to the confocal list when Larry suggested
it and it worked like a charm. I cut and pasted the email address
right from his posting.

LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU

Try again. Cheers, John


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--
C. John Runions, Ph.D.
Department of Plant Sciences
University of Cambridge
Cambridge CB4 1YE
UK

email at work cjr41-at-cam.ac.uk
email at work runions-at-ntlworld.com
Phone (01223) 766 545




From daemon Fri Mar 10 21:19:10 2000



From: Maureen Petersen :      mape-at-gnv.ifas.ufl.edu
Date: Fri, 10 Mar 2000 10:52:41 -0500
Subject: seeking flatbed scanner info

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Dear List Members:

We are in the market for two relatively inexpensive flatbed scanners. One
needs to have a USB attachment and drivers for a Mac. This one is pretty
straightforward. Up to about $200 (US) should suit our needs.

For the second one I am considering one with an adapter to allow scanning of
negatives and 35 mm slides. I have heard a bad report of a scanner not
focussing correctly on the negative or transparency. Does anyone have any
comments to support or refute the focusing problem? We are able to pay up to
$400 if I can find one that performs well. Obviously, we are not considering
high end scanners. I would prefer USB, but could cope with another type of
connection. Thank you for any help you can provide.

Sincerely,
Maureen Petersen
Dept Plant Pathology
University of FL
Gainesville, FL 32611



From daemon Fri Mar 10 21:19:10 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 10 Mar 2000 11:30:53 -0400
Subject: RE:Moving Obj Aperture

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It has been a number of years since I've been directly involved in
operating and maintaining a JEOL 100CX, but as I recall both the specimen
holder and the aperture are inserted into the rather limited space between
the objective pole pieces, with the specimen rod fitting in above the
aperture. If the aperture consistently moves when you move the specimen
holder, then I'd strongly suspect that either the end of your specimen rod
has been bent downward just a bit, or the end of the aperture holder has
been bent upward slightly so that the specimen rod rubs the aperture holder
when it is moved thus causing the aperture to move. The clearance between
these two devices is only a fraction of a millimeter, and so it wouldn't
take much bending to cause this problem.

Good luck,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 10 21:19:10 2000



From: Leah Dobbs :      s20260-at-onsemi.com
Date: Fri, 10 Mar 2000 09:31:58 -0700
Subject: Fw: Fw: Allied Multiprep Polisher for TEM samples

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} } Anita,
} } I have been using the MultiPrep for TEM of semiconductors since
December. It
} } has made my life much much easier. I am responsible for the TEM
} } preparation/imaging/data at ON Semi. Since I have started using the
MultiPrep I
} } have been able to consistently turn a job around in 1 day and less. I
have only
} } failed on 1 sample since December and that was due to my bad judgement.
Once you
} } get a routine for making the samples I have found the results to be very
} } consistent. Do you have an Ion Mill? I use the PIPS and it
contributes to
} } the success of the samples I make. Although if you did not have a PIPS
I think
} } that you would still get very good results using the MultiPrep.
} } For me the major differences between using the MultiPrep and the Tripod
Polisher
} } are:
} } 1. Consistent amount of force placed on the samples using the
MultiPrep.
} } 2. Having the digital micrometer on the MultiPrep takes some of the
guess work
} } out of the sample prep. I am able to tell how much material I am
removing
} } 3. Piece of mind. Since I have developed a set routine I am able to
make 2
} } samples a day and feel confident that they will not fail. Hand
polishing has
} } always been a crap shoot for me.
} } From your signature line I am assuming you work on various materials.
The work
} } I have done is primarily Silicon, but I think that the results would be
good for
} } other materials also. I have submitted an abstract for the MSA
conference this
} } year. I would be happy to speak with you about the system if you will
be
} } attending.
} }
} } Leah L Dobbs
} } ON Semiconductor
} } CSAL TEM
} } 602-244-4820
} } 1-888-637-9415 (6379415-at-skytel.com)
} } s20260-at-onsemi.com
} }
} } My disclaimer " I do not work for Allied High Tech, and do not have
vested
} } interest in the companies financial success. I am merely a satisfied
customer".
} }
} }
} } ----- Original Message -----
} } From: "Anita Garg" {Anita.Garg-at-lerc.nasa.gov}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Thursday, February 24, 2000 5:55 AM
} } Subject: Re: Allied Multiprep Polisher for TEM samples
} }
} }
} }
} ------------------------------------------------------------------------
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America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Colleagues
} } } Does anybody have experience on the Allied MultiPrep Polishing
} } } System, especially in terms of getting a good TEM sample? How well do
} } } the Allied's Precision Cross-Section Tool and TEM Wedge Polisher work
} } } on this semi automatic multiprep polisher?
} } } Also, how does their TEM Wedge Polisher compare with the South Bay
} } } Tripod Polisher?
} } } TIA
} } } Anita
} } } *******************************************
} } } Dr. Anita Garg
} } } NASA Glenn Research Center at Lewis Field
} } } Advanced Metallics Branch
} } } Mail Stop 49-3
} } } 21000 Brookpark Road
} } } Cleveland, OH 44135
} } } Phone : (216) 433-8908
} } } Fax : (216) 977-7132
} } } E-mail : Anita.Garg-at-grc.nasa.gov
} } } *******************************************
} } }
} } }
} }



From daemon Fri Mar 10 21:19:12 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 10 Mar 2000 11:44:23 -0400
Subject: RE: Extraction Replicas

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The extraction replica technique was developed by Bob Fisher at the U. S.
Steel Laboratory back in the early 1950's when we were all trying to
identify the precipitates in various alloy systems for the first time. I
don't seem to be able to lay my hands on the original reference; however,
the technique is described in reasonable detail on pages 115-117 of the
book 'Techniques for Electron Microscopy', Desmond Kay, Ed., Blackwell
Scientific, 1961.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 10 21:19:13 2000



From: brink-at-tiger.3dem.bioch.bcm.tmc.edu
Date: Fri, 10 Mar 2000 10:55:22 -0600 (CST)
Subject: Re: Electron diffaction software

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Hi, we have some software freely downloadable for processing ED of protein
crystals. It's at http://ncmi.bcm.tmc.edu/software.html. Look for packages
auto and edp. The former is a front-end to a bunch of fortran programs
for automated processing, whereas the latter is a GUI-based program.

Jaap

--
Jaap Brink, Ph.D., Biochemistry, One Baylor Plaza, Baylor College of
Medicine, Rm. N420 Alkek Building, Houston, TX 77030
Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu
URL : http://ncmi.bioch.bcm.tmc.edu/~brink

On Fri, 10 Mar 2000, Chengge Jiao wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Does anybody know where I can get free software for electron doffraction and
} more...
}
} Can we download them free ?
}
} Chengge JIAO
}
} H.H.Wills Physics Lab
} University of Bristol
} c.g.jiao-at-bristol.ac.uk
}
}



From daemon Fri Mar 10 21:19:18 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 10 Mar 2000 12:12:08 -0600
Subject: Image Analysis: NIST Particles needed

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Firstly, thanks to everyone who helped out with the earlier question
on image analysis. We are using NIH Image 1.62 (which has a watershed
filter to separate touching particles) to measure their diameters.
(Worked like a charm -- thanks John Russ).

We need a NIST particle, sized 5 µm or so, to use as a standard.
Unfortunately, the standard we recently purchased was sized using
light scattering (which apparently gives a higher reading than
electron microscopy) and our sizing does not agree with that figure.

Does anyone know were we can find 5.0 µm polystyrene particles that
have been sized by TEM and are NIST certified or traceable?

Thanks,

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Mar 10 21:19:21 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 10 Mar 2000 12:47:53 -0600
Subject: binucleate hepatocytes & granules in gallbladder: basic histo

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Fellow microscopists:

Every year when I teach the liver lectures for my histology course, I
explain to the students that up to 25% of the hepatocytes are
binucleate and another 50% are polyploid. These textbook facts match
up with what I see in the scope. But I don't understand why
hepatocytes (or transitional epithelial cells) are frequently
binucleate (or polyploid). Any one able to answer this structure -
function question?

My second histology question concerns the gallbladder epithelium. I
have a great prepared H&E 1.5 um paraffin slide from Carolina
Biological. Most of the epithelial cells have a few fine red-stained
granules in them. I assume these are glycoproteins and/or mucin-type
glycoproteins. A few scattered cells in the epithelium have larger,
intensely eosinophilic granules in them. I can't figure out what
these cells are. None of the standard textbooks or atlases discuss
them. Before anyone suggests they are mucin-filled goblet cells, I
should point out that intestinal goblet cells are my main research
focus and these are unlike any mucin secreting cell I have seen in
the intestine, respiratory tract, stomach, conjunctiva, etc. They
look, in fact, more like Paneth cells in the small intestine. Any
ideas?

Thanks, Tom


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Fri Mar 10 21:19:25 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Fri, 10 Mar 2000 14:23:35 -0500 (Eastern Standard Time)
Subject: Re: Objective Aperture Moving

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It is my understanding that many instances of so-called aperture
thermal drift are actually the result of hysteresis in either the
condenser or objective lenses. This seems to be more of a problem in
JEOL instruments than in others that I'm aware of. It is particularly
pronounced when you spend time scanning large areas with little or no
changes in magnification or brightness. When the current is changed to
either lens, the periphery of an aperture may enter the field of view.
By realigning the aperture, you actually misalign it along the optical
axis. This is why a TEM is often in such abysmal alignment after use
by students and less experienced users. Our JEM 1210 has 9 lenses,
with each one potentially affected by hysteresis. Most of our users
know when it is time to switch off the lens power temporarily and
reload the alignment values. In the case of a less automated
instrument such as the 100-C series, it will often suffice to run C2,
Obj, and Int lenses through their entire ranges before aligning
apertures.



From daemon Fri Mar 10 21:19:29 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 10 Mar 2000 14:55:35 -0500
Subject: Re: TEM: Carbides in tool steels

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The problem with extraction replicas for your carbides is that they will
still be too thick to analyse! Extraction replicas work better the smaller
the carbides (in my humble opinion!). I would recommend mechanical
thinning followed by ion-milling (we typically don't bother with the
intermediate jet-polishing step). Of course, if you also have fine
carbides, you will need the extraction replicas in order to be able to
examine them without interference from the matrix!

Good luck,

Tony Garratt-Reed.


At 10:31 AM 03/10/2000 +0100, you wrote:
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Fri Mar 10 21:19:31 2000



From: Nancy Monteiro :      nancy_monteiro-at-ncsu.edu
Date: Fri, 10 Mar 2000 15:14:18 -0500
Subject: RE: gallbladder: basic histoquestions

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First of all you never indicated from which species this gall bladder
epithelium came from. Goblet cells are characteristic in the bovine
species. The cat epithelium may contain globule leukocytes. Also,
endocrine cells have been reported in bovine.

Nancy A. Monteiro-Riviere, Ph.D., DABFE, DABFM
Professor of Investigative Dermatology and Toxicology
North Carolina State University
College of Veterinary Medicine
Department of Clinical Sciences
Center for Cutaneous Toxicology and Residue Pharmacology
4700 Hillsborough St.
Raleigh, NC 27606
Tel: 919-513-6426
FAX: 919-513-6358
email: Nancy_Monteiro-at-ncsu.edu
CCTRP Homepage: http://cctrp.ncsu.edu

} ----------
} From: Tom Phillips[SMTP:PhillipsT-at-missouri.edu]
} Sent: Friday, March 10, 2000 1:47 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: binucleate hepatocytes & granules in gallbladder: basic
} histoquestions
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Fellow microscopists:
}
} Every year when I teach the liver lectures for my histology course, I
} explain to the students that up to 25% of the hepatocytes are
} binucleate and another 50% are polyploid. These textbook facts match
} up with what I see in the scope. But I don't understand why
} hepatocytes (or transitional epithelial cells) are frequently
} binucleate (or polyploid). Any one able to answer this structure -
} function question?
}
} My second histology question concerns the gallbladder epithelium. I
} have a great prepared H&E 1.5 um paraffin slide from Carolina
} Biological. Most of the epithelial cells have a few fine red-stained
} granules in them. I assume these are glycoproteins and/or mucin-type
} glycoproteins. A few scattered cells in the epithelium have larger,
} intensely eosinophilic granules in them. I can't figure out what
} these cells are. None of the standard textbooks or atlases discuss
} them. Before anyone suggests they are mucin-filled goblet cells, I
} should point out that intestinal goblet cells are my main research
} focus and these are unlike any mucin secreting cell I have seen in
} the intestine, respiratory tract, stomach, conjunctiva, etc. They
} look, in fact, more like Paneth cells in the small intestine. Any
} ideas?
}
} Thanks, Tom
}
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}



From daemon Fri Mar 10 21:19:34 2000



From: RCHIOVETTI-at-aol.com
Date: Fri, 10 Mar 2000 16:11:58 EST
Subject: LM: Leitz Periplan Eyepiece?

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Colleagues,

I am searching for a Leitz Periplan photomicro eyepiece, 10X/18, 23mm
diameter. The old Leitz part number was 519749. The current Leica part
number is 11519749, although it is a discontinued item. This particular
eyepiece has a threaded mount on the viewing end. The thread was used to
mount eyecups on the eyepiece, but it was also used as a photoeyepiece.

If anyone has one of these photo eyepieces and would be willing to part with
it, please contact me off-list.

TIA!

Bob Chiovetti



From daemon Fri Mar 10 21:19:46 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Fri, 10 Mar 2000 16:45:04 -0700
Subject: Re: immunofluorescence in methacrylate

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Your best bet is to embed in paraffin. However, if you still want to use
resin for immunolocalization, you may try Immuno-Bed Embedding resin,
available from Electron Microscopy Sciences. It is a fairly low viscosity
media.

Soumitra


} Dear microscopists,
} Can anyone offer advice on doing immunofluorescence at the light
} level? I
} have cryopreserved plant tissue that I am contemplating embedding and using
} for immunolocalization. I have a protocol that uses butyl, methyl
} methacrylate. I am wondering several things. Why butyl, methyl
} methacrylate? Are there other resins/media that are sufficient? I'd
} appreciate any advice anyone has.
} Kristen Lennon
} Kristen A. Lennon
} Cell, Molecular & Developmental Biology Group
} Department of Botany & Plant Sciences
} University of California
} Riverside, CA 92521
} kalen-at-citrus.ucr.edu




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Fri Mar 10 21:19:47 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 10 Mar 2000 17:57:51 -0600 (CST)
Subject: Re: Rapid Fixation of cells for SEM)

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Robyn & others,

Here are two references more recent than the two I sent earlier regarding
simultaneous glut/osmium fixation methods for single cells in suspension:


Dentler, WL (1999) Fixation of Tetrahymena cells for electron microscopy.
Methods in Cell Biology 62:323-331

but is modified from

Omoto, C.K, and Kung, C. (1980). Rotation and twist of the central pair
microtubules in the cilia of Paramecium . J. Cell Biol 87:33-46



Responding to the message of {v03007805b4ec9d6d6eae-at-[206.69.208.21]}
from R schlicher {rkslick-at-yahoo.com} :

} Hello,
}
} I need to fix cells for viewing with SEM. However,
} the fixation method needs to be consistent with my
} experimental methods:
}
} 1) I need to fix the cells in less than 1 minute and
} due to this time limitation, I cannot spin the cells
} into a pellet and must add the cell suspension to the
} fixative; and
}
} 2)I am looking for "effects" to the cell membrane
} caused by my experiment, so I need to keep any
} possible fixative damage/effects at a minimum.
}
} I have fixed cells using plunge freezing and viewed
} them with TEM, this is time consuming and I ran into
} a
} lot of freeze damage. I would like to try chemical
} fixation and SEM. It has been suggested that I try
} glutaraldehyde + buffers, then postfix with osmium
} tetroxide and dehydrate in an ethanol series.
}
} Is this the best method? I am open to suggestions.
} I am using suspensions of prostate cancer cells in
} RPMI (+serum) growth media; I can perform the
} experiment in buffered saline and use a high
} concentration of cells.
}
} Thanks,
} Robyn
}
}
} Robyn K. Schlicher, M.S.
} Laboratory for Drug Delivery
} Institute for Bioengineering and Biosciences
} Georgia Institute of Technology
} 315 Ferst Drive
} Atlanta, GA 30332
} rkslick-at-yahoo.com

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Fri Mar 10 21:19:50 2000



From: Jianguo Wen :      wenji-at-bc.edu
Date: Fri, 10 Mar 2000 19:11:40 -0500
Subject: TEM textbook

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I am going to give a course on electron microscopy including TEM, SEM, STM,
AFM, etc. to graduate students. Could you give me information on textbooks
about these microscopy.

Thanks

****************************************************
Prof. Jianguo Wen

Department of Physics
Boston College
140 Commonwealth Avenue
Chestnut Hill, MA 02467
Tel: (617) 552-3586 Fax: (617) 552-8478
Email: wenji-at-bc.edu
****************************************************



From daemon Fri Mar 10 21:19:59 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 10 Mar 2000 18:05:53 -0600
Subject: Amray 1910FE SEM maintenance contract

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Are there any Amray-trained service people out there that are
independently servicing the 1910FE system on the West Coast?
I'm in Sacramento CA. I am not looking for someone who knows
how to spell Amray. I am looking for someone who knows what
the 1910FE is all about. Big difference. Introductory questions:

1. Can you condition the emitter/gun? How and with what?
2. How do you handle the differential vacuum aperture?
3. what is your experience in working with the Windows control s/w?
4. What do you know about the IP8?
5. Do you have any knowledge about moving from 486 ISA to P-II
or P-III systems for NibbleNet and frame capture?

Since the gobbling of Amray by KLA-Tencor, Amray SEM service
is dismal--more so day by day. The service people are excellent--but they are being
pulled in all directions, and mostly towards the KLA big semi systems.

The Amray SEM systems were/are really good--and that is why KLA bought
Amray. But KLA is not a research SEM outfit like Amray was.

If I had to get something other than this 1910FE, I don't know what it
would be. The Hitachi and Philips SEMs are great/nice but ridiculously
expensive to buy and maintain. And I do not care for Jeol.

Me thinks that the research SEM options are shrinking daily.

gary g.



From daemon Fri Mar 10 21:20:00 2000



From: Marc Helvey :      Marc.Helvey-at-vlsistd.com
Date: Fri, 10 Mar 2000 18:02:23 -0800
Subject: RE: Image Analysis: NIST Particles needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Bozzola -

Duke Scientific should produce something like this. I know that
they used a TEM to perform their sizing at one time. I'm not sure
if this is the still the case. If not, maybe they can point you
in the right direction. Here's Duke's website as
well as a few other particulate manufacturers:

Duke Scientific:
http://www.dukescientific.com/

Polysciences:
http://www.polysciences.com/pr/index.html

Spherotech:
http://www.spherotech.com/p0000010.htm

Seradyn:
http://www.seradyn.com/

Interfacial Dynamics:
http://www.teleport.com/~idclatex/

Bang's Labs:
http://www.bangslabs.com/

Good Luck -

================================================
Marc W. Helvey
Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
phone:(408) 428-1800, ext. 108
FAX: (408) 428-9555
Mobile: (408) 307-3833
e-mail : marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
internet: http://www.vlsistd.com {http://www.vlsistd.com/}
================================================



From daemon Sat Mar 11 10:01:11 2000



From: Nelson+ Conti :      NelsonC51-at-excite.com
Date: Fri, 10 Mar 2000 20:45:27 -0800 (PST)
Subject: Re: TEM textbook

Contents Retrieved from Microscopy Listserver Archives
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On Fri, 10 Mar 2000 19:11:40 -0500, Jianguo Wen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am going to give a course on electron microscopy including TEM, SEM,
STM,
} AFM, etc. to graduate students. Could you give me information on
textbooks
} about these microscopy.
}
} Thanks
}
} ****************************************************
} Prof. Jianguo Wen
}
} Department of Physics
} Boston College
} 140 Commonwealth Avenue
} Chestnut Hill, MA 02467
} Tel: (617) 552-3586 Fax: (617) 552-8478
} Email: wenji-at-bc.edu
} ****************************************************
}
}
Prof. Wen -
In 1994, I took a course on electron microscopy (for biologists) at San
Francisco State University given by Gregory Antipa (my former M.A. advisor
and principal investigator).
The textbook he used was titled
"Electron Microscopy (:) Principles and Techniques for Biologists" by John
J. Bozzola and Lonnie D. Russell (eds. Jones and Bartlett Publishers, Boston
.. London).
Although this textbook is intended for biology students, it was used in a
class that any graduate student could sign up for given his/her interests.
I found the textbook to be illuminating and interesting to read.
It has a historical chapter on electron microscopy, and then discusses,
among many topics, fixation uses / types of / .. embedding procedures and
types - TEM; similar topics - SEM; microtomy; staining procedures, types,
etc. ; TEM itself; SEM itself; immunocytochemistry and EM ; autoradiography;
freeze-fracture ; analytical electron microscopy (e..g., x-ray microanalysis
subchapter; EELS subchapter; SAD diffraction mode sub-subchapter), and many
other topics relevant to electron microscopy.
Unfortunately .. it does not cover STM (Scanning-Tunneling Microscopy) nor
AFM (Atomic Force Microscopy).
I personally do not know of any textbooks that cover those types of electron
microscopy, but I would guess that someone in this list would have
suggestions. For that matter, I am not even aware if this textbook is still
being published, but perhaps Gregory Antipa (e-mail: antipa-at-sfsu.edu ) can
tell you, or someone else.
I hope that this information may serve as a useful starting point in your
search for a textbook on electron microscopy for graduate students.
Nelson Conti (former graduate student)
San Francisco State University
1600 Holloway Avenue
San Francisco, California (CA) 94132
URL: www.sfsu.edu






_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Sat Mar 11 10:01:12 2000



From: Jeff & Wanda Gray :      jefwan-at-flash.net
Date: Fri, 10 Mar 2000 22:58:09 -0600
Subject: Re: hand sectioning - carbon blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Feather makes the blades we LM histotechs use most often in our everyday
sectioning. They are distributed by Allegiance (used to Baxter, used to be
SP....). Just FYI.
Wanda Shotsberger
(HT ASCP)
-----Original Message-----
} From: Grazyna M Tokarczyk [mailto:gmtokarc-at-is.dal.ca]
Sent: Friday, March 03, 2000 8:26 AM
To: Gordon Couger
Cc: c.jeffree-at-ed.ac.uk; Dan Luchtel; microscopy-at-sparc5.microscopy.com




On Thu, 2 Mar 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------



From daemon Sat Mar 11 10:01:42 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 11 Mar 2000 03:10:50 -0800 (PST)
Subject: Re: Image Analysis: NIST Particles needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,
I worked with characterisation of latex sphere size for many years and
traced them to NIST standard. You may wish to contact Interfacial
Dynamics Corp. as they manufacture spheres and no doubt have the capacity
to trace to NIST. I do not have their address information at hand, but
they are located in Portland, OR.

If you have other questions, please contact me at me University of
Portland email address.

-Ken





From daemon Sat Mar 11 10:01:47 2000



From: Chengge Jiao :      c.g.jiao-at-bris.ac.uk
Date: Sat, 11 Mar 2000 12:59:06 -0000
Subject: EM image simulation software

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Does anybody know the price for following softwares for EM simulation.

1. Diffraction 2.0 (or the most latest version),

2. EMS.

Chengge Jiao

H.H.Wills Physics Laboratory
University of Bristol, UK

c.g.jiao-at-bristol.ac.uk



From daemon Sat Mar 11 10:01:55 2000



From: Jan Leunissen :      leunissen-at-aurion.nl
Date: Sat, 11 Mar 2000 16:28:53 +0100
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tamara,
Without more information about the procedure that was followed it is
hard to suggest a solution. But since the issue of background with
immunogold and enhancement is becoming a regular topic in this List,
it may be worthwhile to address it more in-depth.
In troubleshooting I always find it a good idea to trace down the
origin of the problem logically, rather than shooting in the dark.
In all the years we have been involved in immunogold detection and
silver enhancement, we have always benefited from simple approaches.
These are documented in troubleshooting brochures we use during our
workshops and which describe step-by-step how to proceed to have the
best chance to pinpoint the problem. Anyone who is interested, please
send me an e-mail.

Now just some comments on some of the things you mentioned:

- working on brain slices, you are probably doing pre-embedding. One
of the issues in pre-embedding is penetration. Most of the time gold
conjugates need a longer time to get inside thicker specimens, but
under the proper conditions they do get there. But what often is
easily forgotten is that when it takes a long time for reagents to
penetrate into the slice, it also takes a long time for unreacted
reagents to get washed out again. So washing should be appropriate.

- you mentioned that there is no difference between silver
enhancement and gold enhancement. Have these specimens been incubated
with gold conjugate? What if there is background even when the gold
conjugate incubation step was left out? With any enhancement system
there is a risk of getting background through
(i) autonucleation or
(ii) by the formation of metal particles in the specimen, formed as a
result of the interaction of components in the specimen
(e.g.aldehydes, borohydrid) with the noble metal salts in the
enhancement mix.
This may also be related to the type of metal salt in the enhancement
solution: noble metal salts need only a little push to become
reduced to the metal again and the more noble the metal the smaller
the push it needs. As for judging if autonucleation has occurred, as
long as the enhancement solution is as clear as it was when the
enhancement started, this should not be a real problem.

- background from magnesium. Gallyas and Danscher (and many others)
have looked into many aspects of (auto)metallography, I can give
references if you are interested.

- light sensitivity of enhancement reagents: light sensitivity can
never be reduced to zero. There are tricks that reduce light
sensitivity to a degree where it no longer seriously interferes with
enhancement on particles, but again, these are noble metal salts, and
they need just so much....
In any case, if light would be causing a problem, which I don't think
is very likely whatever brand you used, you should have the same
phenomena as with autonucleation: the mixture should become turbid or
colored after a while. If this is not the case, there is no reason to
be worried about a possible light sensitivity.

Jan
===========================
Jan Leunissen
AURION http://www.aurion.nl
Costerweg 5
6702 AA Wageningen
phone: (31)-317-497676
fax: (31)-317-415955
You will find more tech info on our website.



From daemon Sat Mar 11 10:34:14 2000



From: yu lung chiu :      yl_chiu-at-yahoo.com
Date: Sat, 11 Mar 2000 10:03:51 -0600
Subject: Need help on PEELS

Contents Retrieved from Microscopy Listserver Archives
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Need help on PEELS
}
} To all,
}
} I am working on grain boundary chemistry measurement
} with Digipeels. Try to find out the segregation
} behavior of boron, which is about 0.5at% averagely.
} However, I cannot find any edge for boron in the
} spectrum. As I don't know what's the optimum setting
} for PEELS to check the grain boundary segregation,
if
} you have any idea on this, please let me know.
}
} Thanks,
} Lung
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Sat Mar 11 10:34:18 2000



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Sat, 11 Mar 2000 10:17:35 -0600
Subject: re: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr.Carstensen,

I'd like to point out that by extraction replica you will NOT overcome the
problem of
the carbides' thickness: you will get only a complete separation of them
from matrix. So, according to my experience, the ion-milling would be a
solution in that it could lead to a THINNING of your carbides.

In case there is a possibility to partially dissolve the carbides, by chemical
means, after their extraction, then the extraction replica technique will
work;
but I don't know about such a chemical technique for dissolvind (partially!)
the carbides.

Corneliu Sarbu, Ph.D.
Dept.of Metallurgy and Materials Science
Catholic University of Leuven
Belgium

- - - - - - - - - - - - - - Original Message - - - - - - - - - - - - - -
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,

I have just started on a TEM-project investigating the microstructures of
different tool steels. The steels contain various types of carbides with
different compositions, shapes and sizes. I have started out by making thin
foils using electrolytical jet polishing. I get nice thin foils where I can
study and analyse the martensitic matrix, but the carbides are off course
too thick to be analysed. To overcome this problem I have considered a
combination of electropolishing and ion milling, but I have also heard about
an extraction replication technique. However, I haven't been able to find
any literature on this technique. Have any of you tried this technique
and/or could you point out some literature describing the technique? Also,
if you have other suggestions to deal with the described problem I would be
pleased to hear about them.

Regards, Jesper

----------------------------------------------------
Jesper Vejloe Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
----------------------------------------------------





- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -




From daemon Sat Mar 11 10:46:09 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Sat, 11 Mar 2000 10:30:28 -0600
Subject: RE: Jesper Carstensen/Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jesper:
Extraction replicas are an old technique. I used it 30 years ago.

1. Prepare a lightly etched metallographic specimen.
2. Evaporate with carbon to form a thin film.
3. If you want increased contrast, shadow with Cr at an angle.
4. Score the surface with a razor to form 1 mm rectangles.
5. Etch again until the carbon squares float off.
6. Scoop up the rectangles and immerse in water.The square may flatten
out. If so, it can be mounted on TEM grid and dried by touching the side of
the grid to a piece of filter paper.
7. If the released square curls up when immersed in the rinse water. scoop
it up with a grid and gently lower it into a bath of acetone. It should snap
out into a flat sheet floating on top of the acetone. Again mount on a grid
and dry. If this doesn't work, remove the replica from the acetone bath and
lower it into the water bath so that surface tension flattens out the
replica.

I'm surprised thnat you could not find a description of thsi
technique in the literature. Try some of the older texts on TEM techniques.

Good luck,
Sam Purdy
National Steel Corp. Technical Center
Trenton, MI, USA
} ----------
} From: "Carstensen, Jesper Vejl¿"
} Sent: March 2000 4:31 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: TEM: Carbides in tool steels
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} I have just started on a TEM-project investigating the microstructures of
} different tool steels. The steels contain various types of carbides with
} different compositions, shapes and sizes. I have started out by making
} thin
} foils using electrolytical jet polishing. I get nice thin foils where I
} can
} study and analyse the martensitic matrix, but the carbides are off course
} too thick to be analysed. To overcome this problem I have considered a
} combination of electropolishing and ion milling, but I have also heard
} about
} an extraction replication technique. However, I haven't been able to find
} any literature on this technique. Have any of you tried this technique
} and/or could you point out some literature describing the technique? Also,
} if you have other suggestions to deal with the described problem I would
} be
} pleased to hear about them.
}
} Regards, Jesper
}
} ----------------------------------------------------
} Jesper Vejloe Carstensen
} Research Scientist, M.Sc., Ph.D.
} Materials Research Department
} Risoe National Laboratory
} P.O. Box 49
} DK-4000 Roskilde, Denmark
} Phone: +45 4677 5776
} Fax: +45 4677 5758
} E-mail: jesper.v.carstensen-at-risoe.dk
} Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
} ----------------------------------------------------
}
}
}
}




From daemon Sun Mar 12 07:42:29 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 11 Mar 2000 12:46:08 -0600
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 06:05 PM 3/10/00 , I wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

[snip]

Ability to spell "Amray" is/was not meant to be negative. I have had
bad experience with service people who say they can work on
systems but in reality cannot. I have replaced my Amray 1830
LaB6 system with the Amray 1910FE. The field emission system
is much more complex and horribly more expensive to replace
if not properly handled/serviced. There are special procedures
especially for the FEI 305FE Zr/W gun assembly that must be
followed or the emitter is zapped.

Bad experience was unfortunate with the LaB6 system. I fear it
would be fatal on a field emission system. That is what I was
clumsy in saying.

gary g.



From daemon Sun Mar 12 07:42:31 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Sat, 11 Mar 2000 22:50:26 +0100
Subject: RE:PEELS of boron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First you are going to have to do a bit of reading. I know of one person
who studied the detection limits both experimentally and theoretically in
PEELS recently ( {2 years) and that is Michael Natusch (formerly Cambridge).
He found current detection limits of C in steel was only about two or three
atomic percent (four to six times higher than your boron average). Check
out Micron volume 30 (1999) pp173-183 (Natusch, Humphreys, Menon &
Krivanek) as a starting point and for references.

Even if you do see a boron peak in the spectrum you will have to work out
the signal to noise ratio (SNR) of the edge. The real problem is that you
may see something that isn't there i.e. noise, or conversely you don't see
something that you should see i.e. poor experimental execution.

To optimise the SNR you are going to need a lot of counts from the boundary
itself. One way to see if your probe is on the boundary is to look for a
possible chemical shift in the core loss edges of the matrix material since
the bonding character of the material is different at the grain boundary.

Good luck.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Sun Mar 12 18:24:27 2000



From: S¿ren Albek :      albek-at-dorit.ihi.ku.dk
Date: Sun, 12 Dec 1999 19:29:21 +0100
Subject: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear all on the Microscopy List,
I am looking for good advice concerning surface analysis of metals using
a microscope. The objects are prehistoric tools, jewellery, etc. made
out of bronze, silver or gold (or combinations).
I am interested in traces of the tools/techniques that was used on the
object. The research technique should be non-destructive. The results
will be used in archaeological research.
I am looking for suggestions about what materials to use, what
equipment is suitable/normally available and not too expensive. Is there
anybody out there who deals with these subjects in a modern, industrial
context - and who could tell me some more about, how they do it ?
References for litterature, companies, etc.

With best regards,
Mr. S. Albek



From daemon Sun Mar 12 22:16:47 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 12 Mar 2000 19:53:01 -0600
Subject: Re: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Off hand, I would think that BSE imagine with a SEM might
be useful. The difference between SE and BSE could be
revealing. Without imaging the actual specimens, I can
only speculate. But based on what you are analyzing,
SE, BSE and perhaps EDX analysis can be insightful.

gary g.



At 12:29 PM 12/12/99 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 13 08:07:05 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Mon, 13 Mar 2000 08:47:55 +0000
Subject: Re: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*Date sent: Fri, 10 Mar 2000 10:31:18 +0100
*From: =?iso-8859-1?Q?=22Carstensen=2C_Jesper_Vejl=F8=22?=
* {jesper.v.carstensen-at-risoe.dk}
*Subject: TEM: Carbides in tool steels
*To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Please notice that extracting carbides from the matrix doesn't make
them thinner. So, it seems that your idea about ion milling is right.

Best regards and good luck,

Witold

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Mon Mar 13 08:07:13 2000



From: Mark Reynolds :      efjeld-at-tiac.net
Date: Mon, 13 Mar 2000 09:00:53 -0500
Subject: RE: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
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Hi

We offer training in EM maintanance either through one of our "Monitoring
and Maintaining the EM" short courses or on site as required by the client.

Steve Chapman
Senior Consultant Protrain - for EM Consultancy and Training World Wide
Tel 44+ 1280 814774 Fax 44+ 1280 814007
www.emcourses.com

----- Original Message -----
} From: electron microscope laboratory {emlab-at-udsm.ac.tz}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, March 10, 2000 5:24 AM


a 45 degree laser line across the sample will tell a lot about
the surface. Getting a fine enough beam would be fun.

If some one wants to try it I have a start on the code in C.

Gordon W5RED

G. C. Couger gcouger-at-couger.com Stillwater, OK
www.couger.com/gcouger
"You miss 100 percent of the shots you never take." - Wayne Gretzky



----- Original Message -----
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
To: "Sren Albek" {albek-at-dorit.ihi.ku.dk}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, March 12, 2000 7:53 PM


Dear Dr. Gauler,

The E.FJELD Co. has factory trained service engineers to handle
conventional AMRAY SEM's (LaB6, tungsten). We offer routine service, spare
parts and service agreements on AMRAY SEM's throughout the country.

Your requested is tough... It is extremely difficult, regardless of
manufacturer (Hitachi, Philips, JEOL or KLA), to find experienced service
people for FE technology. We do not supply such service.

If I find a source, I will keep you posted.

Regards,

Mark Reynolds

E.FJELD Co., Inc.
3 Executive Park Drive
No. Billerica, MA 01862

TEL: (978)667-1416 x10
FAX: (978)667-9059





On Friday, March 10, 2000 7:06 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Are there any Amray-trained service people out there that are
} independently servicing the 1910FE system on the West Coast?
} I'm in Sacramento CA. I am not looking for someone who knows
} how to spell Amray. I am looking for someone who knows what
} the 1910FE is all about. Big difference. Introductory questions:
}
} 1. Can you condition the emitter/gun? How and with what?
} 2. How do you handle the differential vacuum aperture?
} 3. what is your experience in working with the Windows control s/w?
} 4. What do you know about the IP8?
} 5. Do you have any knowledge about moving from 486 ISA to P-II
} or P-III systems for NibbleNet and frame capture?
}
} Since the gobbling of Amray by KLA-Tencor, Amray SEM service
} is dismal--more so day by day. The service people are excellent--but
they are being
} pulled in all directions, and mostly towards the KLA big semi systems.
}
} The Amray SEM systems were/are really good--and that is why KLA bought
} Amray. But KLA is not a research SEM outfit like Amray was.
}
} If I had to get something other than this 1910FE, I don't know what it
} would be. The Hitachi and Philips SEMs are great/nice but ridiculously
} expensive to buy and maintain. And I do not care for Jeol.
}
} Me thinks that the research SEM options are shrinking daily.
}
} gary g.
}
}



From daemon Mon Mar 13 17:01:14 2000



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Mon, 13 Mar 2000 09:39:39 -0500
Subject: Re: binucleate hepatocytes & granules in gallbladder: basic

Contents Retrieved from Microscopy Listserver Archives
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Dear Tom,

I know little about liver, but if these are fairly large cells under high
demands to produce [mRNAs], the polyploidy may be a response to a
biological need. In a far simpler system, the nematode, there is good
evidence that ploidy gets regulated in direct correspondance to the
absolute size of the cell, across many different tissues, such that the
ploidy perhaps matches the needs of the cell.

see Hedgecock and White (1985) Dev Biol 107: 128-133.

Though vertebrate cells may or may not be so closely regulated, the
tendency might lie along a similar continuum?

Just a thought. Best regards,
David H. Hall
Center for C. elegans Anatomy
Department of Neuroscience
1410 Pelham Parkway
Albert Einstein College of Medicine
Bronx, NY 10461

phone (718) 430-2195 FAX (718) 430-8821
hall-at-aecom.yu.edu
website: www.aecom.yu.edu/wormem



From daemon Mon Mar 13 17:01:14 2000



From: Ric Felten :      smartech-at-javanet.com
Date: Mon, 13 Mar 2000 10:01:07 -0500
Subject: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


S¿ren,

Check out my web page for examples of the different modes of scanning
electron microscopy. It is a simple web page and has a section showing
examples of the different modes of SEM. I recently quit my job and built a
SEM lab in my basement and also have x-ray microanalysis for generating
elemental spectra on micro-volumes. I am an expert in the different SEM
contrast mechanisms, but not a metallurgist w/ any experience in the field
that you mention. SEM is non-destructive provided you can get the material
into the chamber. Some SEMs have large chambers, mine is on the smaller
side (perhaps samples {4" and somewhat flat sample would be best). I would
be glad to run 2-3 samples for free to show you what SEM can do as a
feasibility study for SEM analysis of your samples. All my output can be in
the form of jpg images so that you can send the data to experts in
archaeology and they can comment on the significance.

Good Luck

Ric Felten
www.semguy.com

-----Original Message-----
} From: S¿ren Albek [mailto:albek-at-dorit.ihi.ku.dk]
Sent: Sunday, December 12, 1999 1:29 PM
To: Microscopy-at-sparc5.microscopy.com


Dear all on the Microscopy List,
I am looking for good advice concerning surface analysis of metals using
a microscope. The objects are prehistoric tools, jewellery, etc. made
out of bronze, silver or gold (or combinations).
I am interested in traces of the tools/techniques that was used on the
object. The research technique should be non-destructive. The results
will be used in archaeological research.
I am looking for suggestions about what materials to use, what
equipment is suitable/normally available and not too expensive. Is there
anybody out there who deals with these subjects in a modern, industrial
context - and who could tell me some more about, how they do it ?
References for litterature, companies, etc.

With best regards,
Mr. S. Albek




From daemon Mon Mar 13 17:01:16 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Mon, 13 Mar 2000 08:47:16 -0800
Subject: Re: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
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In addition to the advice you have already received you might wish to consider contacting your local Forensic Science Laboratory and talk to the Firearm and Toolmark Section. What you wish to do is to determine the Class characteristics of the tools used, as you do not have the actual tool for comparison. There is also some Toolmark analysis done at certain types of machine shops but a really experienced Toolmark examiner at your local Forensic Science Lab would be much easier to locate and probably more interested in your project (I'm currently doing some work for a project on bullets from a sight, in my free evenings and I know others have in the past). Low power stereo microscopy can tell you a lot. The examiners can point you in the right direction and provide some literature sights etc., or may be interested in doing the work for you.

Jim

James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } "S¿ren Albek" {albek-at-dorit.ihi.ku.dk} 12/12/99 10:29AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all on the Microscopy List,
I am looking for good advice concerning surface analysis of metals using
a microscope. The objects are prehistoric tools, jewellery, etc. made
out of bronze, silver or gold (or combinations).
I am interested in traces of the tools/techniques that was used on the
object. The research technique should be non-destructive. The results
will be used in archaeological research.
I am looking for suggestions about what materials to use, what
equipment is suitable/normally available and not too expensive. Is there
anybody out there who deals with these subjects in a modern, industrial
context - and who could tell me some more about, how they do it ?
References for litterature, companies, etc.

With best regards,
Mr. S. Albek






From daemon Mon Mar 13 17:01:19 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 13 Mar 2000 13:28:15 -0500
Subject: double labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I need advice on double labeling (on-grid).
I've had success doing each of the labeling experiments independently but
now the researcher wants to see double labeling. I'm doing indirect
labeling with a monoclonal antibody to fungal cell wall antigens followed
by a gold labeled secondary. In addition, I want to label the sections with
gold conjugated wheat germ agglutinin.
Should I do the antibody labeling first and then the lectin?
I've found one reference that I haven't had a chance to follow up on
(Cailliez et al, 1988) in Immunogold Silver Staining Principles, Methods,
and Applications by Hayat.
Any advice would be greatly appreciated.
Beth




From daemon Mon Mar 13 17:01:19 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Mon, 13 Mar 2000 12:49:42 -0800
Subject: LowicrylK4M

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we routinely use Lowicryl K4M. We normally buy it as a kit from EM
science. They also offer a it as a MonoStep Single Component. We would
like to try it. Has anybody done the comparison between the kit and the
monostep stuff (quality, shelflive, handling, polymerisation)?
Thanks for your time,

Christoph Bauer
University of Chicago



From daemon Mon Mar 13 17:01:23 2000



From: S¿ren Albek :      albek-at-dorit.ihi.ku.dk
Date: Mon, 13 Dec 1999 22:41:22 +0100
Subject: RE:My questions

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
thank you very much for all the kind answers, good ideas, and good
suggestions. It has provided me with a good range of possibilities (I am
a little overwhelmed by now :-). I must now evaluate the answers and I
will most likely return with some more questions - some of these
off-list, when appropriate.

Once again,
thank you for the interest,
and with many regards,

Mr. S.Albek



From daemon Mon Mar 13 17:01:25 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 13 Mar 2000 19:04:46 -0400
Subject: Quantifying the sharpness of glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a question about the cleaved glass blades used to cut cross
sections of copolymers. Is there any way to quantify the sharpness of
the blades? Do they have a radius of curvature, or anything like that?
A client of ours has asked if the blade would be sharp enough to slice
through the hard domain (polystyrene hardness) at -20 C or -80 C,
or if it would just push it out of the way. This also applies to the
disposable blades on a cryostat used to section the same sample at
-20C prior to the cryo-ultramicrotoming. The sample is very brittle
at -120C.
Rosemary

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html




From daemon Tue Mar 14 07:09:08 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 13 Mar 2000 15:14:21 -0800 (PST)
Subject: SEMICaps for SEM

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Hello,
We have an old semicaps 4000 for pc windows 3.1 system. I've been trying
to use it to get scale bars burned into images, but I must say that it is
very buggy. The images never burn scale bars correctly, calibration
procedures are never fully written in the manual, and often minimizing and
maximizing windows causes images to have very 'weird' color schemes.

After contacting semicaps I was told that there were no bug fixes to the
software as it was bug free. Anyone out there have some experience with
this system?
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Tue Mar 14 07:09:09 2000



From: jim :      YZFRJIM-at-ix.netcom.com
Date: Mon, 13 Mar 2000 18:55:58 -0500
Subject: amray 1800 series

Contents Retrieved from Microscopy Listserver Archives
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Does anyone now anybody selling a 1800 series not field emitter, by
amray

thanks,
jim



From daemon Tue Mar 14 07:09:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 13 Mar 2000 17:43:13 -0600
Subject: RE: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My post has generated several responses. All of which are very
helpful. Thanks to all who responded.

From following up on what I have discovered from the response from
Alex Greene, I'm rather stuck. It turns out that Amray has a lock on
the FE gun/emitter that they developed and which incorporate the
FEI W/Zr emitter (305FE). As such, no other party will support the
Amray FE systems.

There are several folks who will and do support the Amray LaB6 systems.
But none can or will support the FE systems. So for the time being,
I am stuck with Amray. Be that as it may. As I write this, Amray
(KLA-Tencor) is preparing to work on my FESEM under my existing
maintenance contract. This is good. I guess that so long as this
keeps up, I am OK. But I do expect that KLA will drop all Amray
field support in the near future. Consequently, perhaps that will
be a better time to seek alternative maintenance sources. I do
expect at this future juncture, KLA/Amray will supply parts
but will not supply warm bodies. If this is an opportunity for
independents, who knows? If you are experiencing similar
troubles, I would for one appreciate hearing about your situation.
Otherwise...

Stay tuned.

gary g.



From daemon Tue Mar 14 07:09:21 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 14 Mar 2000 15:45:28 +1000
Subject: RE: Quantifying the sharpness of glass knives

Contents Retrieved from Microscopy Listserver Archives
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Unless somebody has done that very same thing, one cannot know. However, I am
optimistic that you could section that material with a glass knife.
A new knife should run virtually to a molecular edge, although that finest edge
would not survive the first section. The glass knife is sharp, the question
concerns more its hardness and toughness.
An LKB trainer years ago told me that a glass knife at liquid nitrogen
temperature is almost as hard as is diamond at room temperature. So there is a
good chance that glass could do. Try it.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, March 14, 2000 9:05 AM, Rosemary Walsh [SMTP:rw9-at-psu.edu] wrote:
}
}
} Dear Listers,
} I have a question about the cleaved glass blades used to cut cross
} sections of copolymers. Is there any way to quantify the sharpness of
} the blades? Do they have a radius of curvature, or anything like that?
} A client of ours has asked if the blade would be sharp enough to slice
} through the hard domain (polystyrene hardness) at -20 C or -80 C,
} or if it would just push it out of the way. This also applies to the
} disposable blades on a cryostat used to section the same sample at
} -20C prior to the cryo-ultramicrotoming. The sample is very brittle
} at -120C.
} Rosemary
}
} Rosemary Walsh, Manager
} The Electron Microscope Facility for the Life Sciences,
} A Shared Technology Facility, The Life Sciences Consortium
} 1 South FrearLab
} Penn State University
} University Park, PA 16802
} (814) 865-0212
} rw9-at-psu.edu
} http://www.lsc.psu.edu/stf/em/home.html
}
}



From daemon Tue Mar 14 07:09:31 2000



From: J.Bruyntjes-at-voeding.tno.nl
Date: Tue, 14 Mar 2000 09:51:23 +0100
Subject: IHC on parraffin embedde tissue

Contents Retrieved from Microscopy Listserver Archives
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Dear all

We would like to do immunohistochemistry on rat lung tissue and we are
especially interrested in CD4, CD8 and some macrophage-markers such as ED1
and ED2. But we would like to have good morphology as well. I have found
some articles about IHC (rat and mice) on tissue fixed in
paraformaldehyde-lysine-peroidate and emdedded in low temp paraffin.
But most of these articles are rather old (1985-1990). Is anyone familliar
with these techniques? Is it difficult to repeat and to get similar results?


Joost Bruijntjes
TNO Nutrition and Food Research Institute
Zeist
Holland
E-mail: J.Bruyntjes-at-voeding.tno.nl





From daemon Tue Mar 14 07:09:34 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Mon, 13 Mar 2000 08:47:55 +0000
Subject: Re: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
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Dear Andrew
In principle, sections can be orientated using any two features in
the block that run parallel to each other and normal to the knife
edge. It would be nice to have reference rods included in the block,
and there are various possible approaches to this, but I cannot see
a) a way of meeting both your criterion of providing a dimensional
reference for the assessment of shrinkage, and of orientating the
section b) a method which is generally applicable to all microtomy
techniques.

To measure shrinkage, I think I would be looking at making the
fresh specimen a standard size, following its dimensions through
processing. Two ways of making specimens a standard size occur
to me: 1) Cut slices of the tissue with razor blades mounted side-
by side separated by a spacer. Re-cut the tissue slice to make
strips with square cross section, or dice it into cubes 2) take a
tissue core with a carefully sharpened stainless steel tube
(cannula, large hypodermic needle) and follow the core diameter
through the process.

For section orientation, reference cores or rods can be melted with
a needle (wax, gelatine) or drilled (plastic) normal to the block face.
The holes may be left as voids or filled with a contrasting
embedding material (e.g. coloured wax, resin, gelatine). However
this is all a bit of a fiddle, and becomes either very difficult to
achieve or impossible in ultramicrotomy of small block faces,
ultracryotomy or cryostat sectioning with Tissue-tek or other liquid
embedment.

A general principle for section orientation and alignment that is
applicable to almost all microtomy techniques is to cut a mesa on
the block face of rhomboidal or trapezoidal shape. The mesa is cut
by removing waste with the corner of the knife (which must be
square) to a little more than the thickness of the serial sequence
you wish to cut. Since the edge-facets of the mesa will be
perfectly aligned normal to the block face the sections can be
aligned using any two corners, or if they should be lost/obscured,
by means of the surviving edges.

This may fail with whole-mounts. In this situation, usually there are
features on the periphery of the specimen that can act as reference
marks, and you still have the option to cut the block face into a
trapezoid or make a mesa, or melt holes in the embedding material
with a hot needle, etc.

Finally, a number of digital image analysis packages now offer
fourier-based image correlation for registering images when making
stereo-pairs, for example, and montages. AnalySIS is one of these:

www.soft-imaging.de


Chris

Date sent: Fri, 3 Mar 2000 09:01:49 +1300
To: c.jeffree-at-ed.ac.uk
} From: Andrew McNaughton {andrew.mcnaughton-at-stonebow.otago.ac.nz}


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


*Date sent: Fri, 10 Mar 2000 10:31:18 +0100
*From: =?iso-8859-1?Q?=22Carstensen=2C_Jesper_Vejl=F8=22?=
* {jesper.v.carstensen-at-risoe.dk}
*Subject: TEM: Carbides in tool steels
*To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Please notice that extracting carbides from the matrix doesn't make
them thinner. So, it seems that your idea about ion milling is right.

Best regards and good luck,

Witold

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Tue Mar 14 18:33:08 2000



From: PESTOEM-at-aol.com
Date: Tue, 14 Mar 2000 09:04:52 EST
Subject: Aperture moving-100CX II

Contents Retrieved from Microscopy Listserver Archives
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To all,
thank you for the many suggestions regarding the moving aperture, It is
definitly not the distance between aperture & specimen holder. It was checked
several times and the clearance is there. Also, the aperture was stable
before and is now moving the most in the # 2 position.
I would appreciate any further comments.
Thank you . Peter A. Stolzenberg, Pesto Inc. " pestoem-at-aol.com"



From daemon Tue Mar 14 18:33:11 2000



From: Leroux christine :      leroux-at-univ-tln.fr
Date: Tue, 14 Mar 2000 15:45:03 +0100
Subject: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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hello,
I have to prepare cross sectionning samples, and I do not know where to
buy the small
vise that I need to clamp the specimen when I have glued them (I have
already the
Mbond 610 adhesive).
I would greetly appreciate any information about this thing (I am not even
sure of the
name of it)
thank you in advance
christine

**************************************
Christine leroux
Lab. M.M.I
Universite de Toulon-Var, Bat.R
B.P.132
F-83957 La Garde Cedex
tel: 00 33 (0) 4 94 14 25 07
fax: 00 33 (0) 4 94 14 21 68

**************************************



From daemon Tue Mar 14 18:33:11 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 14 Mar 2000 11:08:19 -0400
Subject: Re: IHC on parraffin embedde tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} Dear all
}
} We would like to do immunohistochemistry on rat lung tissue and we are
} especially interrested in CD4, CD8 and some macrophage-markers such as ED1
} and ED2. But we would like to have good morphology as well. I have found
} some articles about IHC (rat and mice) on tissue fixed in
} paraformaldehyde-lysine-peroidate and emdedded in low temp paraffin.
} But most of these articles are rather old (1985-1990). Is anyone familliar
} with these techniques? Is it difficult to repeat and to get similar results?
}
}
} Joost Bruijntjes
} TNO Nutrition and Food Research Institute
} Zeist
} Holland
} E-mail: J.Bruyntjes-at-voeding.tno.nl

**********
I have had some luck with the PLP fixation you mention (McLean & Nakane, J
Histochem Cytocem 22, 1974). I gives good structural preservation, and like
most immuno techniques, you won't know if it works with your antigen until
you try it. Are you sure you mean low temp paraffin and not resin?
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Tue Mar 14 18:33:11 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Tue, 14 Mar 2000 09:37:07 -0500
Subject: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I'm looking for a source for vibration isolation tables for ultramicrotomy.
The marble tables that we are using now are not adequate (except during
Spring Break when no one is in the building...) I'm thinking of a system
that uses compressed gas (N2?) to float the tables.

Please respond offline (vendors welcome).

thanks, all

Ann Hein Lehman
EM Facility Mgr
Trinity College
Hartford CT
860-297-4289
860-297-2538
ann.lehman-at-trincoll.edu



From daemon Tue Mar 14 18:33:14 2000



From: Jensen, Karen :      Karen_Jensen-at-URMC.Rochester.edu
Date: Tue, 14 Mar 2000 10:53:00 -0500
Subject: Immunoelectron microscopy of myelinated nerve

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I have recently performed an immunoEM labeling procedure with
myelinated nerve embedded in Lowicryl, K4M resin. Unfortunately the myelin
is severely altered (it appears as large clealr blebs) because I am not able
to osmicate this tissue due to the UV polymerization of this resin. Does
anyone have any suggestions as to preservation of lipid laden myelin without
the use of osmium? I used methanol in the dehydration procedure which has
probably caused this artefact....

Thanks!
Karen Jensen, M.S.
Associate Scientist
Electron Microscope Research Imaging Core
University of Rochester Medical Center
Rochester, NY




From daemon Tue Mar 14 18:33:15 2000



From: Ric Felten :      smartech-at-javanet.com
Date: Tue, 14 Mar 2000 11:12:24 -0500
Subject: Confocal Microscopy in North West Ct

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I lost contact w/ a certain person. All I can remember is that they were
experienced in confocal microscopy and lived in North West Ct, I think
Cornwall.

Please respond off line.

Ric
860-491-3299




From daemon Tue Mar 14 18:33:17 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 14 Mar 2000 11:13:05 -0800
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Gary Gaugler wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} My post has generated several responses. All of which are very
} helpful. Thanks to all who responded.
}
} From following up on what I have discovered from the response from
} Alex Greene, I'm rather stuck. It turns out that Amray has a lock on
} the FE gun/emitter that they developed and which incorporate the
} FEI W/Zr emitter (305FE). As such, no other party will support the
} Amray FE systems.
}
} There are several folks who will and do support the Amray LaB6 systems.
} But none can or will support the FE systems. So for the time being,
} I am stuck with Amray. Be that as it may. As I write this, Amray
} (KLA-Tencor) is preparing to work on my FESEM under my existing
} maintenance contract. This is good. I guess that so long as this
} keeps up, I am OK. But I do expect that KLA will drop all Amray
} field support in the near future. Consequently, perhaps that will
} be a better time to seek alternative maintenance sources. I do
} expect at this future juncture, KLA/Amray will supply parts
} but will not supply warm bodies. If this is an opportunity for
} independents, who knows? If you are experiencing similar
} troubles, I would for one appreciate hearing about your situation.
} Otherwise...
}
} Stay tuned.
}
} gary g.
Gary,
Your earlier comments on how good AMRAY is really puzzle me, but be that
as it may, if any of their Federal Government customers are on the ball,
Amray will be told, as was Perkin Elmer ETEC, that they must support the
equipment for about 7 years or face lawsuits. There is an implied
responsibility when one sells expensive equipment.

Perhaps supplying parts and detailed instructions will cover them
legally, in which case we third party folks may be able to help out. If
anyone has any more detailed info on Amray service, I would love to know
because I have serviced them off and on over the years and would be
willing to help out here in the Northeast/Mid-Atlantic area.

Ken Converse
owner
Quality Images
third party SEM service
16 Creek Rd.
Delta, PA 17314

717-456-5491



From daemon Tue Mar 14 18:33:17 2000



From: Grace Kennedy :      kennedy-at-nsi.edu
Date: Tue, 14 Mar 2000 08:23:43 -0800
Subject: IHC on paraffin/CD4

Contents Retrieved from Microscopy Listserver Archives
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Have a look at: Toda, Y et al: Application of tyramide signal
amplification system to immunohistochemistry: A potent method to localize
antigens that are not detectable by ordinary method. Pathology
International 1999; 49: 479-483. They mention CD4 specifically in addition
to other antigens and outline several antigen retrieval methods.



From daemon Tue Mar 14 18:33:18 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 14 Mar 2000 11:36:16 -0500
Subject: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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Dear Christine:

South Bay Technology, Inc. manufactures both a cross sectioning vise
(formerly produced by VCR Group) and also an XTEM clamp. Both tools are
used for the same purpose you describe although they do it a little bit
differently. I am sending you a data sheet covering each one as an
attachment to a separate e-mail. We also offer a complete cross section
sample preparation kit which provides all of the bits and pieces that you
would need to make the cross section.

I hope this helps.

Best regards-

David
Writing at 9:07:17 AM on 03/14/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Leroux christine
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


hello,
I have to prepare cross sectionning samples, and I do not know where to
buy the small
vise that I need to clamp the specimen when I have glued them (I have
already the
Mbond 610 adhesive).
I would greetly appreciate any information about this thing (I am not even
sure of the
name of it)
thank you in advance
christine

**************************************
Christine leroux
Lab. M.M.I
Universite de Toulon-Var, Bat.R
B.P.132
F-83957 La Garde Cedex
tel: 00 33 (0) 4 94 14 25 07
fax: 00 33 (0) 4 94 14 21 68

**************************************

{



From daemon Tue Mar 14 18:33:28 2000



From: Howell, Andy :      ahowell-at-psco.com
Date: Tue, 14 Mar 2000 11:55:10 -0700
Subject: SEM: need BSE detector

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a backscattered electron detector (for elemental contrast)
to attach to a JEOL JSM-5800 SEM. This is for a university, so low-cost or
donation would be most desirable. Thanks for your help!

Andy Howell
(for the) U. of Colorado-Denver



From daemon Tue Mar 14 18:33:28 2000



From: Hao Li :      haoli-at-Glue.umd.edu
Date: Tue, 14 Mar 2000 13:51:54 -0500 (EST)
Subject: Re: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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Hi, Leroux:
I think a negative tweezle will do, provided that your sample is
small. Just make sure that you do not heat
your sample too fast. Actually I designed a spring vice especially for
cross section samples, it is very good for both big and small samples, the
glue line can go thinner than 100nm easily, but
normally I do not use it since almost all of my samples are small---those
film growers are really stinge:) Anyway, if you need, I can send a
schematic to you.

Good Luck.

Hao Li

On Tue, 14 Mar 2000, Leroux christine wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} hello,
} I have to prepare cross sectionning samples, and I do not know where to
} buy the small
} vise that I need to clamp the specimen when I have glued them (I have
} already the
} Mbond 610 adhesive).
} I would greetly appreciate any information about this thing (I am not even
} sure of the
} name of it)
} thank you in advance
} christine
}
} **************************************
} Christine leroux
} Lab. M.M.I
} Universite de Toulon-Var, Bat.R
} B.P.132
} F-83957 La Garde Cedex
} tel: 00 33 (0) 4 94 14 25 07
} fax: 00 33 (0) 4 94 14 21 68
}
} **************************************
}
}




From daemon Tue Mar 14 18:33:33 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Tue, 14 Mar 2000 13:18:28 -0700
Subject: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

Does anyone have a protocol for making Toluidine blue stain to be used for
staining thick sections of resin embedded biological samples ?

Thanks a lot,

Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Tue Mar 14 18:33:33 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Tue, 14 Mar 2000 13:13:17 -0700
Subject: Looking for Microscope Service

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

Can anyone suggest name of an individual or independent company in New
Mexico, West Texas or Arizona who can fix/service research level compound
microscopes ?

Thanks in advance,

Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Tue Mar 14 18:33:35 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Tue, 14 Mar 2000 15:47:52 -0500
Subject: old PGT

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We have an old PGT System 4plus (no detector) sitting here that won't boot.
Apart from that it was working fine. If anyone would be interested in it
for parts (or whatever else you want), they are welcome to it. Not
interested in cash, just pay for your shipping and its yours. We are
located near Ottawa, Canada.

Please contact Mark Chambers at 599-6500 ext. 4269 for more info, or if you
are interested.




From daemon Tue Mar 14 18:33:39 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Wed, 15 Mar 2000 08:43:45 +1100
Subject: IHC on parraffin embedde tissue

Contents Retrieved from Microscopy Listserver Archives
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Can't comment on the paraffin, but PLP certainly gives improved fixation.

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Tue Mar 14 18:33:41 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 14 Mar 2000 17:23:35 -0500
Subject: RE: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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You can buy a nice little vise from South Bay Technology. This vise was
previously made by VCR. It can be put on a hot plate and has Teflon jaws
and a Teflon slide base so that the epoxy doesn't stick. I have two of
these. They work great. I put a small dab of epoxy on t he Teflon jaw to
determine when the epoxy is cured and scraped it off with a glass microscope
slide. SBT also has two spring loaded clamping devices that can be used,
but I recommend the small vise. You can also buy a spring loaded clamping
device from Fischione and from Gatan.
-Scott



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Leroux christine [mailto:leroux-at-univ-tln.fr]
} Sent: Tuesday, March 14, 2000 9:45 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM cross section preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} hello,
} I have to prepare cross sectionning samples, and I do not
} know where to
} buy the small
} vise that I need to clamp the specimen when I have glued
} them (I have
} already the
} Mbond 610 adhesive).
} I would greetly appreciate any information about this thing
} (I am not even
} sure of the
} name of it)
} thank you in advance
} christine
}
} **************************************
} Christine leroux
} Lab. M.M.I
} Universite de Toulon-Var, Bat.R
} B.P.132
} F-83957 La Garde Cedex
} tel: 00 33 (0) 4 94 14 25 07
} fax: 00 33 (0) 4 94 14 21 68
}
} **************************************
}
}



From daemon Tue Mar 14 18:33:41 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 14 Mar 2000 17:27:03 -0500
Subject: RE: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
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Here is the web site for TMC: http://www.techmfg.com/vibrasolutions.html
and http://www.techmfg.com/65floorplatfrm.html
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
} Sent: Tuesday, March 14, 2000 9:37 AM
} To: 'MSA Listserver'
} Subject: Vibration isolation tables
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear Listers,
}
} I'm looking for a source for vibration isolation tables for
} ultramicrotomy.
} The marble tables that we are using now are not adequate
} (except during
} Spring Break when no one is in the building...) I'm thinking
} of a system
} that uses compressed gas (N2?) to float the tables.
}
} Please respond offline (vendors welcome).
}
} thanks, all
}
} Ann Hein Lehman
} EM Facility Mgr
} Trinity College
} Hartford CT
} 860-297-4289
} 860-297-2538
} ann.lehman-at-trincoll.edu
}



From daemon Tue Mar 14 18:33:43 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 14 Mar 2000 14:53:42 -0800
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
}


Toludine Blue Stain

100g Toludine Blue
100g Borax (sodium borate)
1000 mls ddw.

Older stain works better than newer. Filter before use.


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Tue Mar 14 18:33:43 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 14 Mar 2000 16:53:44 -0600
Subject: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
We are trying to get a project started that involves imaging the
microstructure of mineral oils and polyalphaolefins as they solidify. The
solid temperature range for materials like these is approximately -20 to -70
C. Does anyone have experience with these or similar materials. We are
hoping to image the particle structure (crystals) by SEM. Any feedback from
those with similar projects/experiences would be very welcome (on or off the
list). Even better would be references to any published reports of this
kind of material exam.

Thanks,
Brad Huggins
BPAmoco,
Electron Microscopy Lab
hugginbj-at-bp.com



From daemon Tue Mar 14 18:33:45 2000



From: Diana_Papoulias-at-usgs.gov (Diana Papoulias)
Date: Tue, 14 Mar 2000 17:03:10 -0700
Subject: stockard's solution

Contents Retrieved from Microscopy Listserver Archives
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Could someone please send me the recipe for preparing Stockard's
solution?

Thank you,

Diana Papoulias
Fisheries Biologist, Research
Columbia Environmental Research Center
US Geological Survey
4200 New Haven Rd.
Columbia, MO 65201

T:573 876 1902
F:573 876 1896
E:Diana_Papoulias-at-usgs.gov




From daemon Tue Mar 14 18:33:45 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 14 Mar 2000 15:16:17 -0600
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
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At 01:13 PM 3/14/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

[snip]


} Gary,
} Your earlier comments on how good AMRAY is really puzzle me, but be that
} as it may, if any of their Federal Government customers are on the ball,
} Amray will be told, as was Perkin Elmer ETEC, that they must support the
} equipment for about 7 years or face lawsuits. There is an implied
} responsibility when one sells expensive equipment.
}
} Perhaps supplying parts and detailed instructions will cover them
} legally, in which case we third party folks may be able to help out. If
} anyone has any more detailed info on Amray service, I would love to know
} because I have serviced them off and on over the years and would be
} willing to help out here in the Northeast/Mid-Atlantic area.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} 16 Creek Rd.
} Delta, PA 17314
}
} 717-456-5491

I take it that you don't think that Amrays are very good. What aspect
of them do you find lacking? I would tend to agree that the early models
are no comparison to their generations of the last 6-10 years. The FE
systems are particularly good. My 1910FE does a great job being an
FE and having the flat lens. The 1800 series with conical lenses are
not as good, but are required when doing IC wafers.

You have a great point about US Gov users. I know several of them.
When does the 7 years start? From what event or timepoint?
Amray has not stopped providing service. But the word is that they
most likely will in about 2 years....based on their takeover by KLA-Tencor.

One fellow pointed out the proprietary nature of the FE gun. Without
access to this, third party providers cannot work on the systems....at
least not on the gun assembly.

tnx,
gary



From daemon Tue Mar 14 18:33:46 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 14 Mar 2000 17:30:41 -0600
Subject: Polymer imaging - PET microstructure

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Hello again Listers!

My second topic for the day is FESEM or TEM imaging of the
crystalline/amorphous microstructure of poly(ethylene terephthalate), PET.
I have on many occasions had success achieving minimal contrast of such
domains in certain unstained PET polymers in the SEM and FESEM. Recently we
have "almost" been able to observe the crystalline domains with enough
clarity to size them, this by direct examination of polished (microtomed)
surfaces. I would like to pursue the microstructural characterization a
step further by using staining or etching techniques (or some other trick
that some one may have up their sleeve!). In Polymer Microscopy (L
Sawyer, D Grubb) aminolysis appears to be (cautiously) recommended.
Staining with PTA is also mentioned. I am looking to image crystalline
domains in the range of approximately 50 to 150 nm.

Does anybody on the List have first hand experience with these or other
techniques to enhance the direct (or indirect) observation of these domains
in PET. I'm not looking to re-invent the wheel here, so any help will be
greatly appreciated. Get back to me direct or on the List.

Again,
Thanks in advance,
Brad Huggins
BPAmoco,
Electron Microscopy Lab
hugginbj-at-bp.com



From daemon Tue Mar 14 18:33:46 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 14 Mar 2000 15:55:52 -0800 (PST)
Subject: Re: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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} hello,
} I have to prepare cross sectionning samples, and I do not know where to
} buy the small
} vise that I need to clamp the specimen when I have glued them (I have
} already the
} Mbond 610 adhesive).
} christine

You haven't told us what equipment you will use to prepare your "cross
sectionning samples", so it's hard to provide advice!


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Tue Mar 14 18:33:47 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Tue, 14 Mar 2000 16:12:13 -0800 (PST)
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would suspect Elaine is giving a 10X stock solution?

We use 1% Toluidine Blue dissolved in 1% sodium borate. Filter before use.
Diluting 10-fold
with 1% sodium borate, to 0.1% toluidine blue, allows lightly
counterstaining sections developed for autoradiography.

Refs.
Mercer, E.H., J Royal Microscopy Society, 81:179 (1963)
Burns, W.A., "Thick Sections: Technique and Applications", Diagnostic
Electron Microscopy, Chapter 4, B.F. Trump and R.J. Jones, eds., John
Wiley and Sons, New Yourk , 1978.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

} } Dear Microscopists,
} }
} } Does anyone have a protocol for making Toluidine blue stain to be used for
} } staining thick sections of resin embedded biological samples ?
} }
} } Thanks a lot,
} }
} } Soumitra
} }
} }
}
}
} Toludine Blue Stain
}
} 100g Toludine Blue
} 100g Borax (sodium borate)
} 1000 mls ddw.
}
} Older stain works better than newer. Filter before use.
}
}
} Dr. Elaine Humphrey
} Biosciences Electron Microscopy Facility
} University of British Columbia
} 6270 University Blvd, mail-stop Botany
} Vancouver, BC
} CANADA, V6T 1Z4
} Phone: 604-822-3354
} FAX: 604-822-6089
} e-mail: ech-at-unixg.ubc.ca
}
}
}
}



From daemon Tue Mar 14 18:33:47 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 14 Mar 2000 16:15:47 -0800
Subject: RE: SEMICaps for SEM

Contents Retrieved from Microscopy Listserver Archives
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Gordon writes ...

} We have an old semicaps 4000 for pc windows 3.1 system.
} I've been trying to use it to get scale bars burned
} into images, but I must say that it is very buggy. ...

First, I need to admit no experience with this system ... but even
with my software which does produce accurate scale bars, I still
prefer to annotate the images with an image editor like Photoshop.

If you have a magnification standard, then you should be able to
determine an instrumental constant which can be assumed doesn't change
with magnification, but may change with the SEM's working distance.
You should be able to end up with this equation:

microns per pixel = constant/magnification

This may need to be determined for each fixed working distance, or
you may be able to determine how to incorporate WD into the equation
(my own software compensates with the objective lens setting so the
mag is relatively accurate). If your image acquisition options
include pixel dimentions (e.g., 256x256, 512x512, 1024x1024), then
this needs to be a factor as well (in the denominator).

I then created a spreadsheet for the pixel dimentions of common scale
bars for different magnifications, printed it, and then made it
available at the Photoshop workstation.

I can provide an example of the spreadsheet to anyone interested.

cheerios, shAf

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - ICQ 210524
Geological Science's Electron Probe Facility - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/





From daemon Tue Mar 14 18:33:48 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 14 Mar 2000 16:19:14 -0800
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Glen, you are quite right. It is easier to make up 10g Toluidine
Blue, 10g sodium borate and 1000mls water.
Elaine

} I would suspect Elaine is giving a 10X stock solution?
}
} We use 1% Toluidine Blue dissolved in 1% sodium borate. Filter before use.
} Diluting 10-fold
} with 1% sodium borate, to 0.1% toluidine blue, allows lightly
} counterstaining sections developed for autoradiography.
}
} Refs.
} Mercer, E.H., J Royal Microscopy Society, 81:179 (1963)
} Burns, W.A., "Thick Sections: Technique and Applications", Diagnostic
} Electron Microscopy, Chapter 4, B.F. Trump and R.J. Jones, eds., John
} Wiley and Sons, New Yourk , 1978.
}
} Regards,
} Glen
}
}
} Glen MacDonald
} Research Scientist
} Hearing Research Laboratories of the
} Virginia Merrill Bloedel Hearing Research Center
} Box 35-7923
} University of Washington
} Seattle, WA 98195-7923
} (206) 616-4156
} glenmac-at-u.washington.edu
}
} } } Dear Microscopists,
} } }
} } } Does anyone have a protocol for making Toluidine blue stain to be used for
} } } staining thick sections of resin embedded biological samples ?
} } }
} } } Thanks a lot,
} } }
} } } Soumitra
} } }
} } }
} }
} }
} } Toludine Blue Stain
} }
} } 100g Toludine Blue
} } 100g Borax (sodium borate)
} } 1000 mls ddw.
} }
} } Older stain works better than newer. Filter before use.
} }

Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Tue Mar 14 18:44:00 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Tue, 14 Mar 2000 16:36:44 -0800 (PST)
Subject: Best way to maintain SEM/TEM facilities

Contents Retrieved from Microscopy Listserver Archives
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We have a FEI CM20 (TEM) and XL30 (FEGSEM) and are in the process of
reviewing service contracts vs insurance. The service contract runs a min
of 40K for both instruments. We currently have a tech that can do minor
service, yearly maintenance, and alignments.

I'd appreciate any input as to how other organizations have dealt with
this.

Thank you,

Ben Craft





From daemon Tue Mar 14 20:31:31 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 14 Mar 2000 19:27:24 -0600
Subject: TEM: LR-White polimerization

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}
} Dear Collegues
}
} I'm having a problem with LR-White polimerization.
}
} I use to polimerise the LR-white resin at 60ŒC without any aditives and it
} usually works without problems. A new batch of the resin failed to
} polimerise, and the manufacturer claims that it is necessary to use the
} catalyst.
}
} I understand that the "accelerator" should not be used except for cold
cure,
} and even then may be harmfull since the specimen may be subject to too
much
} heat even under those conditions.
}
} I would like your opinion on:
} 1. is it necessary to use the catalyst for heat cure?
}
} 2. If it is, is there any difference among the resins that may justify the
} apparent success of the old protocol (good cure without catalyst?)
}
} Thanks in advance
}
} Dr. A.P. Alves de Matos
}
}
}




From daemon Tue Mar 14 20:31:34 2000



From: Meyers, Greg (GF) :      GFMEYERS-at-dow.com
Date: Tue, 14 Mar 2000 20:43:41 -0500
Subject: Scanned Probe Microscopist Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position: SCANNED PROBE MICROSCOPIST

Full-time
The Dow Chemical Company, Midland, Michigan with possible relocation to
Dow's Freeport, Texas facility
Equal opportunity employer offering a competitive compensation and benefits
package including 401k, stock purchase, performance incentives, and
educational assistance

Responsibilities:
Support existing businesses using SPM and develop new customers for SPM as
appropriate.
Calibrate and maintain SPM instrumentation base consisting of Digital
Instruments' Multimode SPM, D3000 SPM, Hysitron Nanoindentation and TA
Instruments Micro-TA SThM.
Participate in business aligned, original research programs through internal
development funding.
Bring in new technology through previous experience or external contacts as
appropriate.

Qualifications:
MS degree in Polymer Science, Materials Science or Chemistry. Ph.D. degree
is preferred.
Experience in scanning probe microscopy characterization of polymeric
materials.
Experience with Digital Instruments Nanoscope is desired.
Routine experience with a variety of imaging modes (e.g. TappingMode, phase,
friction) as well as unscanned modes (force curve, nanoindentation) is also
desired.
Leading research in the polymer SPM area.


Send Resumes by April 10, 2000 to:
The Dow Chemical Company, P. O Box 1655,
Workforce Planning Department Job 00-94/MLK, Midland, Michigan, United
States, 48641-1655 or e-mail: R&D-at-dow.com. Email respondents must list Job
00-94/MLK and their last name as the first and second items on the Subject
line. Only those selected for an interview will be contacted.

________________________________________________________________________
The Dow Chemical Company is a global science and technology based company
that develops and manufactures a portfolio of chemical, plastic and
agricultural products and services for customers in 168 countries around the
world. With annual sales of more than US$18 billion, Dow conducts its
operations through 14 global businesses employing 39,000 people. The
company has 123 manufacturing sites in 32 countries and supplies more than
3,500 products. The 39,000 Dow people around the world develop solutions
for society based on Dow's inherent strength in science and technology -
which we refer to as "good thinking." Good thinking helps customers
succeed, stockholders prosper, employees achieve and communities thrive.
For more news and information about Dow, please visit our web site at
www.dow.com.
________________________________________________________________________


Gregory F. Meyers, Ph.D.
The Dow Chemical Company
Analytical Sciences
1897E Building
Midland, MI 48667
phone: 517-636-4149
FAX: 517-638-6443
e-mail: gfmeyers-at-dow.com




From daemon Tue Mar 14 20:31:35 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 14 Mar 2000 18:18:00 -0800
Subject: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
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1% Toluidine Blue in 1x PBS pH 7.5:

Weight 0.5 g Tolyidine Blue in some bottle (I am using 50 ml polypropylene
tissue culture tubes), add 50 ml 1x PBS and heat it in boiled water for
20-30 min. When hot, filter it using Whatman filter paper (Cat No 1001 090).

Staining:
Plastic sections up to 0.5 microns thick on the microscope slide cover with
several drops of the stain and heat on heat-plate, 60oC for 5-10 min. Wash
away stain with tap water, wash in distilled water, dry and enjoy the view.

Godd luck, Sergey.


} Date: Tue, 14 Mar 2000 13:18:28 -0700
} From: Soumitra Ghoshroy {ghoshroy-at-nmsu.edu}
} Subject: Toluidine blue stain
} X-Sender: ghoshroy-at-cnmailsvr.nmsu.edu
} To: Microscopy-at-sparc5.microscopy.com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Tue Mar 14 20:47:59 2000



From: Ben Craft :      bcraft-at-mercury.oac.uci.edu
Date: Tue, 14 Mar 2000 18:43:58 -0800 (PST)
Subject: Best way to maintain SEM/TEM facilities

Contents Retrieved from Microscopy Listserver Archives
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We have a FEI CM20 (TEM) and XL30 (FEGSEM) and are in the process of
reviewing service contracts vs insurance. The service contract runs a min
of 40K for both instruments. We currently have a tech that can do minor
service, yearly maintenance, and alignments. We have not had a service
contract for the last 2 years and have had no problem getting FEI to come
out ASAP when needed.

I'd appreciate any input as to how other organizations have dealt with
this.

Thank you,

Ben Craft




From daemon Wed Mar 15 02:30:45 2000



From: A. P. Alves de Matos :      apmatos-at-ip.pt
Date: Qua, 15 Mar 2000 08:22:48 +0000
Subject: RE: Aperture moving Jeol

Contents Retrieved from Microscopy Listserver Archives
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I have a (possibly) similar problem with JEOL 100SX microscope.
Very often the beam keeps tilting in a motion that displaces it relative to the objective aperture. The effect is
the same as moving the aperture.
I suspect of circuit instability but the assistance was unable to find any trouble in that area.
Another possibility is charge accumulation inducing electric fields and displacing the beam. Since you are
lucki to have the problem mostly in one position, I would suspect of some kind of electric isolation of the
aperture holder either in that hole or produced when the entire piece goes into that position.
The charge effects could be also related to the specimen itself. Jeol seems to be particularly sensitive to this.
If you are looking at epon sections try to coat them with a thin layer of carbon (after staining, evaporate directly
into the sections) - it gives much improvement for me.

Dr. A.P. Alves de Matos



To all,
thank you for the many suggestions regarding the moving aperture, It is
definitly not the distance between aperture & specimen holder. It was checked
several times and the clearance is there. Also, the aperture was stable
before and is now moving the most in the # 2 position.
I would appreciate any further comments.
Thank you . Peter A. Stolzenberg, Pesto Inc. " pestoem-at-aol.com"






From daemon Wed Mar 15 02:40:43 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 15 Mar 2000 18:32:59 +1000
Subject: RE: LR-White polimerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most LR White is sold with catalyst already added. This will cure at 60 degrees
and must be stored refrigerated. If you purchased uncatalysed (the only way we
supply LR White because we are a long way from the UK) then prior to first use
the catalyst must be added to the whole bottle and well mixed to dissolve.
Thereafter store the catalysed LR White refrigerated.

If you want to cold cure you need to additionally add accelerator. Accelerator
is not supplied with a bottle of LR White, but when you purchase a "kit".
Instruction notes are linked online from the LR White.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, March 15, 2000 11:27 AM, A.P. Alves de Matos [SMTP:apmatos-at-ip.pt]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} }
} } Dear Collegues
} }
} } I'm having a problem with LR-White polimerization.
} }
} } I use to polimerise the LR-white resin at 60oC without any aditives and it
} } usually works without problems. A new batch of the resin failed to
} } polimerise, and the manufacturer claims that it is necessary to use the
} } catalyst.
} }
} } I understand that the "accelerator" should not be used except for cold
} cure,
} } and even then may be harmfull since the specimen may be subject to too
} much
} } heat even under those conditions.
} }
} } I would like your opinion on:
} } 1. is it necessary to use the catalyst for heat cure?
} }
} } 2. If it is, is there any difference among the resins that may justify the
} } apparent success of the old protocol (good cure without catalyst?)
} }
} } Thanks in advance
} }
} } Dr. A.P. Alves de Matos
} }
} }
} }
}
}



From daemon Wed Mar 15 04:10:30 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Wed, 15 Mar 2000 10:51:10 +0100
Subject: TEM: moving apertures on 100CX II

Contents Retrieved from Microscopy Listserver Archives
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I do not know what the objective aperture strip looks like on a 100 CX so I
am sort of guessing.

I have seen some aperture rods where the end consists of say three or four
holes of identical diameter and small Pt/Mo discs with different sized
holes etched in.
If the No2 aperture is moving the most then this could possibly explain it.
This disc maybe more loosely held or has a worse connection to ground than
the others. Can you check these with a multimeter or something?

What does the movement look like:- continuous slow/ slow with fast jumps/
rapid shaking/random direction/ one direction: along the rod or side to
side? And does the total beam current affect the motion greatly?

********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Wed Mar 15 04:20:29 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 15 Mar 2000 10:15:57 +0000
Subject: Non-shrinking resin

Contents Retrieved from Microscopy Listserver Archives
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Dear All

I need to embed some complex glass mouldings which also
contain components made of plastics (HDPE, PP, PC) in (for
preference) clear resin for sectioning with a diamond saw. This has
turned out to be much more problematical than I had expected, for
two reasons:

1) Very considerable shrinkage of the resins occurs during curing
(applies to acrylic, epoxy or polyester types) . This causes
separation of the resin from the glass and plastics components
inside partially-enclosed voids, so that the original spatial
relationships are lost.

2) bonding of the resins to the glass is quite variable, but typically
poor. Bonding to plastics is poor. When the bond-strength to glass
is high, particularly with epoxies, the stresses induced by the
shrinking resin during curing are large enough to fracture the glass.

Is there such a thing as a non-shrinking resin?
How can I improve bond-strength between glass and resin?
How can I improve bond-strength between resin and HDPE or PP?

Any tips gratefully received
Yours
Chris
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Wed Mar 15 04:30:27 2000



From: John :      cjr41-at-cam.ac.uk
Date: Wed, 15 Mar 2000 10:18:38 +0000
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Soumitra,

To stain resin, e.g. Spurr's, toluidine blue must be prepared at high pH.

0.5% toluidine blue in 0.1%sodium carbonate (Na2CO3) (pH 11.1) works well.
Stain sections on slides on a hot plate at 60C for 30sec. to 2 min. and rinse
with water. Some resins and plastics are much easier to stain than Spurr's,
e.g. JB-4, GMA etc. so I also use 0.1% tol blue in 0.01% sodium carbonate (pH
6.6). The basic protocol stays the same, you vary concentrations to adjust
pH and therefore staining intensity. Hope this helps, John.

Soumitra Ghoshroy wrote:

} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
} *****************************************************************
} Soumitra Ghoshroy Ph.D.
} Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3600
} Fax: 505-646-5665
} e-mail: ghoshroy-at-nmsu.edu
} http://confocal.nmsu.edu/eml

--
C. John Runions, Ph.D.
Department of Plant Sciences
University of Cambridge
Downing St.
Cambridge
CB2 3EA
UK

email cjr41-at-cam.ac.uk
phone (01223) 766 545




From daemon Wed Mar 15 04:30:27 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 15 Mar 2000 10:03:14 +0000 (GMT)
Subject: Re: Aperture moving-100CX II

Contents Retrieved from Microscopy Listserver Archives
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Hi Peter,

From your comments I would suspect that you have a charging
problem. As it seems to be dependent on the aperture chosen it may be on
the aperture mechanism. Try cleaning it, check that there is electrical
continuity along the rod and that the mechanism is properly grounded. Do
you have a touch switch? If so then ensure that it is grounded
through the resistor properly.

Good luck,
Ron


On Tue, 14 Mar 2000 PESTOEM-at-aol.com-at-sparc5.microscopy.com wrote:}

} thank you for the many suggestions regarding the moving aperture, It is
} definitly not the distance between aperture & specimen holder. It was checked
} several times and the clearance is there. Also, the aperture was stable
} before and is now moving the most in the # 2 position.
} I would appreciate any further comments.
} Thank you . Peter A. Stolzenberg, Pesto Inc. " pestoem-at-aol.com"
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Wed Mar 15 06:50:06 2000



From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 15 Mar 2000 12:43:55 +0000 (GMT)
Subject: Re: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bradley:

I know oil based fuels have been imaged at low temperature. Suggest you
read (better still, Buy) my book "Low Temperature Microscopy and
Analysis" Plenum Press New York 1992. I would modestly suggest it has a
lot of information about how to prepare, observe and analyse specimens
at low temperature. My own current interests revolve around low volyage,
low temperature x-ray microabalysis of bio-organic materials. Let me
know if I can be of any further help

Patrick Echlin
University of Cambridge UK

pe13-at-cus.cam.ac.uk


On Tue, 14 Mar 2000, Huggins, Bradley
J wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello all,
} We are trying to get a project started that involves imaging the
} microstructure of mineral oils and polyalphaolefins as they solidify. The
} solid temperature range for materials like these is approximately -20 to -70
} C. Does anyone have experience with these or similar materials. We are
} hoping to image the particle structure (crystals) by SEM. Any feedback from
} those with similar projects/experiences would be very welcome (on or off the
} list). Even better would be references to any published reports of this
} kind of material exam.
}
} Thanks,
} Brad Huggins
} BPAmoco,
} Electron Microscopy Lab
} hugginbj-at-bp.com
}
}
}



From daemon Wed Mar 15 07:30:04 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 15 Mar 2000 08:25:42 -0500
Subject: Re: LR-White polimerization

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From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 15 Mar 2000 08:25:42 -0500
Subject: Re: LR-White polimerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You have fallen into the same trap I did some time back. Some suppliers ship the LR White resin with the catalyst already included. Others do not, sending the catalyst along with instructions to add it prior to using the resin. The reason to not mix the ingredients prior to shipping is that if the resin is subject to hot temperatures or prolonged shipping time, the resin mix could be compromised. This is a good point which is certanly valid when shipping resin in the summer or to remote locations.

However, most of us do not run into these problems and are used to receiving the resin in the complete mixed form. In my case,I ordered from a different supplier than normal. The labeling of the bottles was not clear and I did not realize that the enclosed "extra" ingredient was the catalyst, not the accelerator used for cold curing polymerization, until I also ran into the problem of the resin not polymerizing as expected.

Moral of the story....always read the fine print...both in the catalog and after receiving the product, especially when ordering from a different supplier than in the past.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

On Tuesday, March 14, 2000, A.P. Alves de Matos {apmatos-at-ip.pt} wrote:
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From daemon Wed Mar 15 08:14:17 2000



From: Buddy Steffens :      steffens-at-calc.vet.uga.edu
Date: Wed, 15 Mar 2000 09:10:26 -0500
Subject: Re: Immunoelectron microscopy of myelinated nerve

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I have recently performed an immunoEM labeling procedure with
} myelinated nerve embedded in Lowicryl, K4M resin. Unfortunately the
} myelin is severely altered (it appears as large clealr blebs) because I
} am not able to osmicate this tissue due to the UV polymerization of
} this resin. Does anyone have any suggestions as to preservation of
} lipid laden myelin without the use of osmium? I used methanol in the
} dehydration procedure which has probably caused this artefact....

Karen,

It sounds like the myelin is being partially extracted during dehydration
because of lack of OsO4 stabilization. You might try acrolein...it is the only
fixative other than OsO4 that will preserve and retain lipids. Make sure that
you read all the precautions about it...its pretty nasty stuff.




-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602 USA
http://www.vet.uga.edu/vpp/wls/steffens.html



From daemon Wed Mar 15 08:34:34 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 15 Mar 2000 10:47:23 -0400
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
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At 1:18 PM -0700 3/14/0, Soumitra Ghoshroy wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

After your sections have been dried onto the slide, cover them with a drop
of stain for 30 sec - 1 min. and wash off with water. Dry.

If its too light, repeat.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed Mar 15 08:34:34 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 15 Mar 2000 09:22:08 -0500
Subject: Re: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Huggins, Bradley J wrote:

} We are trying to get a project started that involves imaging the
} microstructure of mineral oils and polyalphaolefins as they solidify. The
} solid temperature range for materials like these is approximately -20 to -70
} C. Does anyone have experience with these or similar materials. We are
} hoping to image the particle structure (crystals) by SEM. Any feedback from
} those with similar projects/experiences would be very welcome (on or off the
} list). Even better would be references to any published reports of this
} kind of material exam.
}
}

Dear Bradley,
Doug Dorset has done extensive work with electron crystallography
of waxes and lipids. He does electron diffraction with a TEM, but perhaps
his results will be useful to you.
Yours,
Bill Tivol



From daemon Wed Mar 15 09:04:12 2000



From: Anne Heller :      heller-at-Uni-Hohenheim.DE
Date: Wed, 15 Mar 2000 15:50:31 +0100
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
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Dear Soumitra Ghoshroy,

for methacrylate sections we use 0.5 % Toluidinblue in 0.1 mol phosphate
buffer (it should also work just with water) and for epoxide sections 0.1%
Toluidinblue in 2% Na-bicarbonate. staining time depends on temperature,
thickness, and material. After staining wash with water and dry the sections.

regards,
Anne


Soumitra Ghoshroy schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
} *****************************************************************
} Soumitra Ghoshroy Ph.D.
} Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3600
} Fax: 505-646-5665
} e-mail: ghoshroy-at-nmsu.edu
} http://confocal.nmsu.edu/eml

--
Dr. Anne Heller, AG Elektronenmikroskopie,
Institut fŸr Botanik (210), UniversitŠt Hohenheim,
Garbenstra§e 30
D-70593 Stuttgart

Tel.: (0049)-711-459-2180
Fax.: (0049)-711-459-3355




From daemon Wed Mar 15 09:34:08 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 15 Mar 2000 10:24:13 -0500
Subject: RE: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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I forgot another cheaper alternative that I have used in the past. You can
use a small, medium, or large binder clip to clamp samples together
depending on their size. You can use Teflon tape on the surface to prevent
sticking to the clamp. If you make a little Teflon jig to hold the sample
it works quite well.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Leroux christine [mailto:leroux-at-univ-tln.fr]
} Sent: Tuesday, March 14, 2000 9:45 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM cross section preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} hello,
} I have to prepare cross sectionning samples, and I do not
} know where to
} buy the small
} vise that I need to clamp the specimen when I have glued
} them (I have
} already the
} Mbond 610 adhesive).
} I would greetly appreciate any information about this thing
} (I am not even
} sure of the
} name of it)
} thank you in advance
} christine
}
} **************************************
} Christine leroux
} Lab. M.M.I
} Universite de Toulon-Var, Bat.R
} B.P.132
} F-83957 La Garde Cedex
} tel: 00 33 (0) 4 94 14 25 07
} fax: 00 33 (0) 4 94 14 21 68
}
} **************************************
}
}



From daemon Wed Mar 15 10:24:03 2000



From: teresa_boes-at-hp.com
Date: Wed, 15 Mar 2000 08:17:38 -0800
Subject: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann,
I'm also looking for vibration isolation tables for ultramicrotomy.
I've been looking at Vibraplane (http://www.kineticsystems.com) for isolation
tables and bench-top isolation platforms, which are also sold through stereo
equipment companies (i.e. http://www.soundsofsilence.com
http://www.audioodyssey.com). I need a height-adjustable isolation table -
other vibration sensitive equipment in our lab is successfully being used on the
isolation platforms on top of separate height-adjustable tables. I would
appreciate hearing of any information you receive offline about the isolation
tables.

Teresa Boes
Analytical Services Lab
Hewlett-Packard
Corvallis, OR
541-715-7055
teresa_boes-at-hp.com

-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Tuesday, March 14, 2000 6:37 AM
To: 'MSA Listserver'


Dear Listers,

I'm looking for a source for vibration isolation tables for ultramicrotomy.
The marble tables that we are using now are not adequate (except during
Spring Break when no one is in the building...) I'm thinking of a system
that uses compressed gas (N2?) to float the tables.

Please respond offline (vendors welcome).

thanks, all

Ann Hein Lehman
EM Facility Mgr
Trinity College
Hartford CT
860-297-4289
860-297-2538
ann.lehman-at-trincoll.edu



From daemon Wed Mar 15 10:33:59 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Wed, 15 Mar 2000 11:32:00 -0500
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Date: Wed, 15 Mar 2000 11:15:59 -0500
} To:Soumitra Ghoshroy {ghoshroy-at-nmsu.edu}
} From:sherwood-at-HELIX.MGH.HARVARD.EDU (Peggy Sherwood)
} Subject:Re: Toluidine blue stain
}
} } Soumitra,
}
} We use a 0.5% Toluidine Blue in Borate Buffer (1 gram Toluidine Blue, 1
} gram sodium borate and 200ml water). Tousimis (and maybe other suppliers)
} sells a Methylene Blue- Toludine Blue stain for epoxy stain (Cat. # 4166B)
} which we have used: (consists of 0.2% methylene blue & 1.0% toluidine
} blue certified stains in Na-Borate buffer). Either way--we always filter
} our stains (using a syringe with filter unit attached) right onto
} sections. Then we put slide ( super frost plus, so sections will adhere)
} on hot plate (temp. -at- 60 decrees C). We rinse sections with water and
} then check stain--time is arbitrary: depending upon tissue type & section
} thickness as to how dark or light your stain result.
}
} When I worked in Ophthalmology research, I used a wonderful dichromatic
} stain: methylene blue-azure II-basic fuchsin stain. It was similar to H
} & E but for epoxy sections. (made great 2X2 slides). The reference is:
} Humphrey, C.D. and Pittman, F.E. (1974) Stain Technology 42:9-14. I
} actually have a copy that came as an LKB application note 303 which I
} could fax to you. Feel free to contact me off line. Good luck!
}
} Peggy Sherwood
} Wellman Labs of Photomedicine
} Lab Associate-Photopathology Lab
} Massachusetts General Hospital
} 50 Blossom Street
} Boston, MA 02114
} 617-726-6983
} 617-724-4839 (voice mail)
} 617-726-3192 (fax)
} sherwood-at-helix.mgh.harvard.edu
}
}
}
}
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Mar 15 11:43:49 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Wed, 15 Mar 2000 10:35:17 -0700
Subject: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all of you who responded to my question about the stain.

This is a great microscopy resource.

Best wishes.


Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Wed Mar 15 11:53:49 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 15 Mar 2000 12:43:55 -0500
Subject: toluidine blue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soumitra,
We use the following recipe for toluidine blue:


These stock solutions can be made in large volumes and stored.
.5 % toluidine blue in distilled water
.25 % sodium borate in distilled water

Mix equal parts of the above.
Stain dried resin sections on hot plate (low setting) until edge of stain
begins to evaporate (5-10 secs)
Rinse, blot excess water from bottom of slide and dry on hot plate.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky




From daemon Wed Mar 15 11:53:51 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Wed, 15 Mar 2000 11:53:12 -0600
Subject: RE: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Patrick,
Are you telling me that in your book, you have a reference to some work
where some oil based fuels were cooled to a solid, and them imaged in that
frozen state? If so, this is precisely the kind of information that I would
like to see. If not, do you have a reference? I do not have a copy of
your book, but I have seen it, some years ago. In any case, thanks for the
reminder on that resource.

And even more specifically, if anyone has knowledge of any similar work done
on mineral oil and like materials... it sure would save me some work on the
development side...

Thanks for the feedback,
Brad

} ----------
} From: Dr P. Echlin[SMTP:pe13-at-cus.cam.ac.uk]
} Sent: Wednesday, March 15, 2000 6:43 AM
} To: Huggins, Bradley J
} Cc: Microscopy listserver
} Subject: Re: Cryo-microscopy of solid mineral oils
}
} Dear Bradley:
}
} I know oil based fuels have been imaged at low temperature. Suggest you
} read (better still, Buy) my book "Low Temperature Microscopy and
} Analysis" Plenum Press New York 1992. I would modestly suggest it has a
} lot of information about how to prepare, observe and analyse specimens
} at low temperature. My own current interests revolve around low volyage,
} low temperature x-ray microabalysis of bio-organic materials. Let me
} know if I can be of any further help
}
} Patrick Echlin
} University of Cambridge UK
}
} pe13-at-cus.cam.ac.uk
}
}
} On Tue, 14 Mar 2000, Huggins, Bradley
} J wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello all,
} } We are trying to get a project started that involves imaging the
} } microstructure of mineral oils and polyalphaolefins as they solidify.
} The
} } solid temperature range for materials like these is approximately -20 to
} -70
} } C. Does anyone have experience with these or similar materials. We
} are
} } hoping to image the particle structure (crystals) by SEM. Any feedback
} from
} } those with similar projects/experiences would be very welcome (on or off
} the
} } list). Even better would be references to any published reports of this
} } kind of material exam.
} }
} } Thanks,
} } Brad Huggins
} } BPAmoco,
} } Electron Microscopy Lab
} } hugginbj-at-bp.com
} }
} }
} }
}



From daemon Wed Mar 15 12:03:47 2000



From: Jose Maria Manero :      manero-at-cmem.upc.es
Date: Wed, 15 Mar 2000 18:59:22 +0100
Subject: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
to study the dislocations substructure. But I have some problems, for
example, I can not focus the image due to the magnetism of the ferrite.

I am looking for suggestions to avoid this problem. What could I do?

Thank you in advance.

Dr. J. M. Manero
Universidad PolitŽcnica de Catalunya.
E.T.S.I. Industriales de Barcelona. Spain.
e-mail: manero-at-cmem.upc.es




From daemon Wed Mar 15 12:03:47 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 15 Mar 2000 12:54:46 -0500
Subject: service contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ben,
If you have other microscopes on campus you might consider forming a
consortium of all the users and hiring your own on-site service engineer
to take care of all the instruments. We've been doing this since 1981 and
it's worked very well. One man's salary is considerably less than service
contracts on many instruments. You also have the luxury of instant service.
Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Wed Mar 15 12:23:47 2000



From: rgriffin-at-eng.uab.edu
Date: Wed, 15 Mar 2000 12:13:38 -0600
Subject: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1) Start (before final thinning) with the thinnest sample you can support
( {50 microns). Pure iron will be quite soft so you'll have to be very
careful not to bend it - especially since you're looking at dislocation
structure. You could support it on a copper key-grid to minimize damage. If
you have to electropolish while it is on the copper key-grid, you'll need to
lacquer the copper prior to electropolishing and then clean it off before
putting it into the microscope.
2) If you still can't focus, try taking it out of the microscope and
rotating it in the holder. Sometimes a different orientation really helps.
3 Plan on doing alignment every time you move or tilt. It is a given with
magnetic samples. You'll need to adjust the beam tilt and the astigmatism
continually. I always used the "dark-field" beam tilts as they are stronger
than the bright field set. You'll be amazed at how good you get at aligning
after a few months of magnetic work. Later work with non-magnetic samples
will be a breeze.
4) One final warning. The objective lens can actually suck your sample out
of the holder during insertion of the sample. If you have a really thin
sample, it can break off chunks. These chunks/sample will ALWAYS stick to
the lens and make every other user miserable. Your service guy will be
forced at some point to clean off the lens of your sample (or sample bits)
and you will be reviled. A simple solution is to always turn off the
objective lens when you are inserting and removing your sample.

Good luck - magnetic work is possible and fun once you get the hang of it.

Robin Griffin

-----Original Message-----
} From: Jose Maria Manero [mailto:manero-at-cmem.upc.es]
Sent: Wednesday, March 15, 2000 11:59 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,

I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
to study the dislocations substructure. But I have some problems, for
example, I can not focus the image due to the magnetism of the ferrite.

I am looking for suggestions to avoid this problem. What could I do?

Thank you in advance.

Dr. J. M. Manero
Universidad PolitŽcnica de Catalunya.
E.T.S.I. Industriales de Barcelona. Spain.
e-mail: manero-at-cmem.upc.es



From daemon Wed Mar 15 13:05:38 2000



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Wed, 15 Mar 2000 14:56:20 -0400
Subject: LM: Fluorescence lamp instability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We've been having stability problems with our mercury vapor lamps on
our Reichert Polyvar microscope. We have been told by one source that the
power supply is likely the problem and another source tells us that the
bulbs are the problem. The source illumination, when imaged, can be seen to
flicker at random intervals and causes the illumination to vary. Does
anyone have experience with this microscope, and if so which brand of lamps
do you use? This microscope uses a 220W/4 L1 lamp. Thanks.


David O'Neil
National Research Council of Canada
Institute for Marine Biosciences
1411 Oxford Street
Halifax, NS B3H 3Z1
ph: (902)426-8258
fax: (902)426-9413
e-mail: david.o'neil-at-nrc.ca



From daemon Wed Mar 15 13:13:36 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 15 Mar 2000 14:07:40 -0500
Subject: RE: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin,

I certainly agree that EM of magnetic samples is quite doable, but I'm not
sure I'd agree that it is "fun".

Perhaps the best tip I can give is to reduce the amount of magnetic
material within the polepiece. I have cut my magnetic samples down to less
than the standard 3mm size and glued them securely onto a Cu slot
grid. The magnetic effects are much less of a problem.

Cheers,
Henk


At 12:13 PM 3/15/00 -0600, rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com wrote:
} {snip}

} Good luck - magnetic work is possible and fun once you get the hang of it.
}
} Robin Griffin

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Murphy's Law: "If anything can go wrong, it will."
Commentary: "Murphy was an optimist."



From daemon Wed Mar 15 13:43:33 2000



From: George Lawton :      George.Lawton-at-email.swmed.edu
Date: Wed, 15 Mar 2000 13:34:49 -0600
Subject: Toluidine blue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soumitra,
We use a Toluidine Blue and Methylene blue staining solution:

Dissolve 0.5gm Borax in 95 ml DH2O, add 0.5gm of methylene blue, mix then add 5 ml of a 2% Toluidine blue solution in H2O.

Filter the stain just before staining. Stain for 1 minute on a hot plate set slightly lower than where the stain starts to bubble.
Rinse with DH2O and dry.

We like this stain because it is fast, very clean, and very pretty. We prefer this stain to
other Toluidine Blue solutions.

George

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu



From daemon Wed Mar 15 14:13:30 2000



From: Ron L'Herault :      lherault-at-bu.edu
Date: Wed, 15 Mar 2000 13:56:47 -0500
Subject: OT Spectrophotometer- B & L

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A lab on our floor is having trouble with their B & L spectrophotometer.
does anyone know who, hopefully in New England, might be able to service
the unit?

Thanks.

Ron
mailto:lherault-at-bu.edu



From daemon Wed Mar 15 14:43:27 2000



From: SEMTRADER-at-aol.com
Date: Wed, 15 Mar 2000 15:41:23 EST
Subject: Wanted TN 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers,

Our old TN2000 (Tracor) nimbin pulse processor has bit the dust. If anyone
has one available for free, please let me know.

Thank you
Keith Brenna
609-658-3908



From daemon Wed Mar 15 16:53:05 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Wed, 15 Mar 2000 17:48:02 -0500
Subject: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1. Reduce the sample size as much as possible, glue a tiny Fe piece on a Cu
or other nonmagnetic grids, for example.
2. Turn off the objective lens as you load your sample.
3. Do beam alignment as many times as necessary.
-cy
Rodel Inc.
451 Bellevue Road
Newark, Delaware 19713
USA


-----Original Message-----
} From: Jose Maria Manero [mailto:manero-at-cmem.upc.es]
Sent: Wednesday, March 15, 2000 12:59 PM
To: Microscopy-at-sparc5.microscopy.com


Dear all,

I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
to study the dislocations substructure. But I have some problems, for
example, I can not focus the image due to the magnetism of the ferrite.

I am looking for suggestions to avoid this problem. What could I do?

Thank you in advance.

Dr. J. M. Manero
Universidad PolitŽcnica de Catalunya.
E.T.S.I. Industriales de Barcelona. Spain.
e-mail: manero-at-cmem.upc.es




From daemon Thu Mar 16 08:06:24 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Thu, 16 Mar 2000 12:27:55 +1100
Subject: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ann,

A simple construction we used for years with a heavy microscope was a
wooden frame standing on a tennis balls. The microscope was on the
2nd floor of a building by a busy road. Never a shake was recorded on
film!

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Thu Mar 16 08:06:30 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 15 Mar 2000 19:12:46 -0800
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As an independent service provider of several SEM manufacturers for
over 20 years,
I think I can also comment & agree with Ken on several fronts concerning
Amray.
However, I don't want to get into a "bashing" of any given manufacturer,
at least online.
I will say that every Company (SEM or otherwise) has their own way of
doing things.
Each Company is different, depending upon the people running the
operation.

Many manufacturer's think they have a "lock" on the customer and on the
service because of their FE guns or perhaps on another part.
This reminds me of ETEC just before they got out of the SEM service
business.
ETEC had an epoxy encapsulated high voltage power supply that only ETEC
manufactured.
I inquired with ETEC about the cost of one of these units. ETEC
responded with a price tag of $50,000.00.
Yes $50,000.00 in 1982!
ETEC service also let it be known that they were the only source for
this part.
Furthermore, customers considering a third party for service were
informed (and threatened) by ETEC about this situation.
Customers were stuck paying ETEC's exorbitant maintenance costs.

The ETEC customers were hesitant to go to a third party service until
the high voltage issue had been resolved.
I spent many days (and nights) re-engineering another high voltage power
supply until I had it perfectly duplicated.
When customers would ask about the high voltage issue, I would show them
my version & invite them to use it in their system.
Within two years I had over 50% of ETEC's service business at 60% of
ETEC contract costs.

Today several manufacturers still think they have a "lock" on service.
I have been informed by one manufacturer that this is their way of
"putting you (third party maintenance) out of business".
If I had the time and the inclination, I would spend it duplicating the
FE gun some of these manufacturer's think they have a "lock" on.
I have considered such a move because no one has a "lock" on technology.

I have had several requests but have not responded because the time &
effort necessary is not viable for only three customer requests..
Fortunately for the manufacturer, I am busy and, quite frankly, not as
"hungry" as I used to be. Maybe it comes with age.
If I ever get "unhungry" or not as busy, I would take these
manufacturer's up on their smugness and beat them at their own game by
duplicating their FE guns.
The ultimate winner is the customer.

The only manufacturer that has a great gun that can be serviced by the
customer is Hitachi.
Hitachi has the patent on an internal heater for their gun which makes
exchanging FE guns obsolete.
Hitachi FE guns can be re-conditioned by the customer in-house at a cost
of $750.00 instead of $7,000.00 for Amray or $17,000.00 for JEOL.
Hitachi is doing well and doesn't need to worry about "locking" third
party maintenance or anyone else out.
They have too many sales to worry about & don't have the time to be
petty.

Great thing about the free enterprise system is that real talent always
has customers "beating a path" to their door.
Marginal talent needs to cajole customers to their way of thinking.
Great talent has customers calling on them.


Regards,

Earl Weltmer
Scanservice Corporation
Third Party Maintenance


At 01:13 PM 3/14/00 , you wrote:


} -----------------------------------------------------------------------.

[snip]

} Gary,
} Your earlier comments on how good AMRAY is really puzzle me, but be
that
} as it may, if any of their Federal Government customers are on the
ball,
} Amray will be told, as was Perkin Elmer ETEC, that they must support
the
} equipment for about 7 years or face lawsuits. There is an implied
} responsibility when one sells expensive equipment.
}
} Perhaps supplying parts and detailed instructions will cover them
} legally, in which case we third party folks may be able to help out.
If
} anyone has any more detailed info on Amray service, I would love to
know
} because I have serviced them off and on over the years and would be
} willing to help out here in the Northeast/Mid-Atlantic area.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} 16 Creek Rd.
} Delta, PA 17314
}
} 717-456-5491

I take it that you don't think that Amrays are very good. What aspect

of them do you find lacking? I would tend to agree that the early
models
are no comparison to their generations of the last 6-10 years. The FE

systems are particularly good. My 1910FE does a great job being an
FE and having the flat lens. The 1800 series with conical lenses are
not as good, but are required when doing IC wafers.

You have a great point about US Gov users. I know several of them.
When does the 7 years start? From what event or timepoint?
Amray has not stopped providing service. But the word is that they
most likely will in about 2 years....based on their takeover by
KLA-Tencor.

One fellow pointed out the proprietary nature of the FE gun. Without
access to this, third party providers cannot work on the systems....at

least not on the gun assembly.

tnx,
gary



From daemon Thu Mar 16 08:06:33 2000



From: Joss, Michael :      Michael.Joss-at-dpr.csiro.au
Date: Thu, 16 Mar 2000 14:45:25 +1100
Subject: FM:Non-fluorescent epoxy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are trying to examine oil inclusions trapped in quartz under UV light.
The oil fluoresces and the quartz does not. Sounds easy. Unfortunately the
epoxy that the sample is embedded in also fluoresces quite strongly making
identification difficult. So I was wondering if there are any
non-fluorescent epoxies available on the market, or alternatively if there
are any pigments/dyes that can be added to the epoxy to reduce its
fluorescence. If not then perhaps an alternative to embedding in epoxy might
be the best option....

Any suggestions warmly welcomed.
:-)

Michael Joss
Quantitative Microscopist
CSIRO Division of Petroleum Resources
Fluid History Analysis Group
Phone: 9490 8148
Fax: 9490 8467



From daemon Thu Mar 16 08:06:37 2000



From: Joss, Michael :      Michael.Joss-at-dpr.csiro.au
Date: Thu, 16 Mar 2000 15:21:18 +1100
Subject: FM:Non-fluorescent epoxy

Contents Retrieved from Microscopy Listserver Archives
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} We are trying to examine oil inclusions trapped in quartz under UV light.
} The oil fluoresces and the quartz does not. Sounds easy. Unfortunately the
} epoxy that the sample is embedded in also fluoresces quite strongly making
} identification difficult. So I was wondering if there are any
} non-fluorescent epoxies available on the market, or alternatively if there
} are any pigments/dyes that can be added to the epoxy to reduce its
} fluorescence. If not then perhaps an alternative to embedding in epoxy
} might be the best option....
}
} Any suggestions warmly welcomed.
} :-)
}
} Michael Joss
} Quantitative Microscopist
} CSIRO Division of Petroleum Resources
} Fluid History Analysis Group
} Phone: 9490 8148
} Fax: 9490 8467
}



From daemon Thu Mar 16 08:06:37 2000



From: Joss, Michael :      Michael.Joss-at-dpr.csiro.au
Date: Thu, 16 Mar 2000 15:23:36 +1100
Subject: Non-fluorescent epoxy

Contents Retrieved from Microscopy Listserver Archives
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} We are trying to examine oil inclusions trapped in quartz under UV light.
} The oil fluoresces and the quartz does not. Sounds easy. Unfortunately the
} epoxy that the sample is embedded in also fluoresces quite strongly making
} identification difficult. So I was wondering if there are any
} non-fluorescent epoxies available on the market, or alternatively if there
} are any pigments/dyes that can be added to the epoxy to reduce its
} fluorescence. If not then perhaps an alternative to embedding in epoxy
} might be the best option....
}
} Any suggestions warmly welcomed.
} :-)
}
} Michael Joss
} Quantitative Microscopist
} CSIRO Division of Petroleum Resources
} Fluid History Analysis Group
} Phone: 9490 8148
} Fax: 9490 8467
}



From daemon Thu Mar 16 08:06:37 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, March 15, 2000 10:25 PM
Subject: Vibration isolation tables

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Dear Diana,

The tennis balls will isolate vibration nearly perfect. We used this
approach a lot in Russia simply because we had no other choice. But tennis
balls have tendency to deflate under pressure of the weight of the machine.
The deflation will become noticeable in a few moths. Then the entire frame
has to be lifted in order to replace tennis balls. Much better approach is
to use pneumatic antivibration mounts by Barry Controls. They come in
different sizes for loads from 100 lb. to several tons, and available from a
number of suppliers including McMaster-Carr.
Unless, of course, Australian tennis balls are superior to Russian ones...
Cheers.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
-----Original Message-----
} From: Diana van Driel {dianavd-at-eye.usyd.edu.au}
To: MicroscopyList {microscopy-at-sparc5.microscopy.com}



From daemon Thu Mar 16 08:06:38 2000



From: EM Unit (HISTO) :      emunit-at-mail.wch.sa.gov.au
Date: Thu, 16 Mar 2000 15:29:00 +1030
Subject: Scanning EM.

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We are interested in obtaining a second hand scanning EM. within Australia
at minimal cost. Please email us personally.

Thanks in advance,

Ruth and Alvis



From daemon Thu Mar 16 08:06:40 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 16 Mar 2000 08:26:58 +0000 (GMT Standard Time)
Subject: Re: [TEM] Problem with magnetic samples

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Hi,

I have read the previous suggestions and would
agree with them - the message is get as much of that magnet
out of your microscope as possible. However other tips that
you may find useful:

If you have a side entry stage you will find it
very difficult to set the eucentric height with a magnetic
specimen. Either note the objective lens current at
eucentric focus and set that or focus a non magnetic
specimen at eucentric and then insert your secimen. Having
done that focus the specimen using the eucentric height
control.

Try to have as small a hole as possible in your specimen.
This will reduce the effect of having a magnet one side of
the beam and not the other. Of course the best specimen
would not have a hole but just uniform thin area.

You will have to realign the beam every time you move the
specimen.

When out of focus you may see the Fresnel image of Domain
walls, when they change contrast from black to white (or
vice versa) then you are in focus.

Good luck,
Ron


On Wed, 15 Mar 2000 18:59:22 +0100 Jose Maria Manero
{manero-at-cmem.upc.es} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
} to study the dislocations substructure. But I have some problems, for
} example, I can not focus the image due to the magnetism of the ferrite.
}
} I am looking for suggestions to avoid this problem. What could I do?
}
} Thank you in advance.
}
} Dr. J. M. Manero
} Universidad Politécnica de Catalunya.
} E.T.S.I. Industriales de Barcelona. Spain.
} e-mail: manero-at-cmem.upc.es
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Mar 16 08:06:44 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 16 Mar 2000 21:47:41 +1000
Subject: RE: Toluidine blue stain

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I've read most of the replies but found no mention of differentiation. This is
one of the most attractive features of the toluidine stain as it can give a two
colour, pink and blue rendition. This shows cell features similar to a double
staining with Haematoxylin & Eosin.

After staining on a hotplate add a drop of acid alcohol and then rinse the
slide with distilled water. The acid alcohol can destain partially or even
completely, but a brief application brings forth the two colours.

Make acid alcohol by adding to 25ml of 50% Ethanol a couple of drops of 1N HCl.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, March 15, 2000 6:18 AM, Soumitra Ghoshroy
[SMTP:ghoshroy-at-nmsu.edu] wrote:
}
} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
} *****************************************************************
} Soumitra Ghoshroy Ph.D.
} Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3600
} Fax: 505-646-5665
} e-mail: ghoshroy-at-nmsu.edu
} http://confocal.nmsu.edu/eml



From daemon Thu Mar 16 08:06:45 2000



From: Heisel, Christian, Dr. :      Christian.Heisel-at-ok.uni-heidelberg.de
Date: Thu, 16 Mar 2000 12:52:08 +0100
Subject: Subject: SEM - Problems with marking bacteria

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We are examining Staphylococcus epidermidis on the surface of acrylic bone
cement. We have problems with the marking of bacteria. Artefacts of
particles from the Polymethylmethacrylate (bone cement) look nearly the same
than the bacteria. We fix with glut, dehydrate, do critical point drying and
coat with gold afterwards. Does anyone have experience with marking bacteria
in SEM to surely identify them?
Thank you in advance,
Christian Heisel, M.D.
University of Heidelberg
Dep.of Orthopedics
Schlierbacher Landstra§e 200 A
69118 Heidelberg
Germany
Phone:0049-6221-965-
Fax:0049-6221-966121
e-mail:christian.heisel-at-ok.uni-heidelberg.de



From daemon Thu Mar 16 08:06:48 2000



From: Alexander Mironov :      amironov-at-nki.nl
Date: Thu, 16 Mar 2000 13:38:40 +0100
Subject: Re: Immunoelectron microscopy of myelinated nerve

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} I have recently performed an immunoEM labeling procedure with
} } myelinated nerve embedded in Lowicryl, K4M resin. Unfortunately the
} } myelin is severely altered (it appears as large clealr blebs) because I
} } am not able to osmicate this tissue due to the UV polymerization of
} } this resin. Does anyone have any suggestions as to preservation of
} } lipid laden myelin without the use of osmium? I used methanol in the
} } dehydration procedure which has probably caused this artefact....

Karen,

Try to switch to cryosections. This reference could help in solving your problem.
Liou W, Geuze HJ, Slot JW Histochem Cell Biol 1996 Jul;106(1):41-58
--
Alexander Mironov Jr.
Division of Tumor Biology, H4
The Netherlands Cancer Institute
Plesmanlaan 121
1066 CX Amsterdam

Tel. +31-020-512-2017
Fax +31-020-512-2029
E-mail: amironov-at-nki.nl




From daemon Thu Mar 16 08:06:50 2000



From: Leslie Eibest :      leibest-at-duke.edu
Date: Thu, 16 Mar 2000 08:22:42 -0500
Subject: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
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Hi, folks;

One concern raised by a granting agency about our proposal
for new equipment for a multi-user SEM lab was our lack of a
management plan (primarily for time allocation among users and for
conflict resolution). We have been operating under a first
come-first served policy, and conflicts have not been a problem.
I would greatly appreciate insights from managers of
multi-user facilities of any sort as to your approaches to allocation
of access to instrumentation, and methods of resolving conflicts
among researchers.

Thanks for any information!

Leslie Eibest
Zoology Dept.
Duke University



From daemon Thu Mar 16 08:06:50 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Thu, 16 Mar 2000 10:26:09 +0000
Subject: looking for parts to TEM Philips EM 201

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Hello, microscopist's.......
I 'm looking for any provider of the parts listed above for an old Philips TEM
EM 201

PW6543 Plate numbering device
PW 6542 automatic exposure kit

any help is welcome......

thanks.....

fernando
===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Thu Mar 16 08:06:51 2000



From: Lab. de Microscop’a :      microsc-at-fi.uner.edu.ar
Date: Thu, 16 Mar 2000 10:46:09 +0000
Subject: looking for parts to TEM Philips EM 201

Contents Retrieved from Microscopy Listserver Archives
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Hello, microscopist's.......
I 'm looking for any provider of the parts listed above for an old
Philips TEM
EM 201

PW6543 Plate numbering device
PW 6542 automatic exposure kit

any help is welcome......

thanks.....

fernando




From daemon Thu Mar 16 08:06:52 2000



From: Robin Schaublin :      Robin.Schaeublin-at-psi.ch
Date: Thu, 16 Mar 2000 14:54:56 +0000
Subject: Re: [TEM] Problem with magnetic samples

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The technique we now use on Fe and ferritic steels allows getting
an excellent behaviour in the TEM:

-A 1 mm hole is punched in the centre of a 3 mm TEM disk made out
of stainless steel (e.g. SS 316) about 300 micron thick,
-A 1 mm disk is punched from our ferritic specimen (about
300 micron thick),
-This 1mm disk is inserted - slightly forced - in the hole of
the 3 mm disk,
-A drop of glue (epoxy) is applied (to make sure there is no leak
during the electropolish that follows).
The 3 mm disk holding the 1 mm disk can then be used as a
normal TEM disk:
-Mechanical polish down to about 50-80 microns,
-Electropolishing with a 10 % vol. perchloric/methanol solution, 18V,
0C.

The final specimen is strong and the remaining magnetism is
minimal in the TEM: we can now move and tilt around without too much
realignment. Note that a puncher of high quality is essential. The one
from 'Eckert' doesn't introduce visible deformation in the centre
of the specimen; we tested that in annealed pure Fe.

-- -
Robin Schaeublin
CRPP - EPFL
5232 Villigen PSI
SWITZERLAND
Tel + 41 56 310 40 82
Fax + 41 56 310 45 29
Robin.Schaeublin-at-psi.ch
http://psgi05.psi.ch/
http://www.ssom.ch/




From daemon Thu Mar 16 17:42:15 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 16 Mar 2000 08:17:39 -0600
Subject: RE: Fluorescence lamp instability

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Dear David:
We have 15 year old Reichert Polyvar which, so far, has given us
little trouble. We use Osram HLX Xenophot bulbs, 12V, 100 W. Oddly enough,
we also have a Reichert MeF3 metallograph, about 10 years old.The light on
this instrument flickers erraticly. We replaced the lamp control board with
no success, switched lamps, etc. To no avail.Our service man thinks the
trouble lies in the transformer, which is no longer available. We are buying
a new separate light source but have not received it yet. We are not happy
with Reichert/Leica concerning assistancein this problem. They have been
less than helpful. On the other hand the service man(Dave Olney) from W.
Nuhsbaum, McHenry IL, has been very helpful.
I would be interesrted in whatever solution to this problem that you
may come up with. How old is your instument?

I have financial interest in either Reichert/Leica or W. Nuhsbaum.
} ----------
} From: O'Neil, David
} Sent: March 2000 1:56 PM
} To: Microscopy Listserver
} Subject: LM: Fluorescence lamp instability
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} We've been having stability problems with our mercury vapor lamps on
} our Reichert Polyvar microscope. We have been told by one source that the
} power supply is likely the problem and another source tells us that the
} bulbs are the problem. The source illumination, when imaged, can be seen
} to
} flicker at random intervals and causes the illumination to vary. Does
} anyone have experience with this microscope, and if so which brand of
} lamps
} do you use? This microscope uses a 220W/4 L1 lamp. Thanks.
}
}
} David O'Neil
} National Research Council of Canada
} Institute for Marine Biosciences
} 1411 Oxford Street
} Halifax, NS B3H 3Z1
} ph: (902)426-8258
} fax: (902)426-9413
} e-mail: david.o'neil-at-nrc.ca
}




From daemon Thu Mar 16 17:42:16 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Thu, 16 Mar 2000 10:54:47 -0400
Subject: Re: Multi-user facility managers

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}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University
} Leslie -

My method, FWIW, is simplicity itself. I have a scheduling calendar posted
on the outer door of the lab, with a pen provided. Clients simply sign
themselves up in whatever day/time slot they wish, provided it's still
open. They can even sign up for days/times in future months, if they so
desire. Haven't caught anyone erasing a previous booking yet....but then
that's why I use a pen.....
This method certainly favours the folks who have their act together far
enough in advance so that they can schedule the instruments at their most
convenient times. Clients who show up at the last minute looking for
openings at their prefered time may well be out of luck. That's what we
call a learning experience.
I'm sure there is software available that can be mounted on your local
network which would do exactly the same thing, but, you know, I think it
makes more of a commitment for the client to actually make a trip down to
the lab to physically sign up, and then they're less likely to forget about
the scheduled session later.
It's worked pretty well for me for about 5 years now, and haven't seen a
good screaming match yet.


Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Thu Mar 16 17:42:26 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 16 Mar 2000 12:36:41 -0400
Subject: Re: Multi-user facility managers

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} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University

*****************************
Dear Leslie,
I have run a multi-user EM facility for 12 years, a now also manage an
optical microscopy facility. Both are core facilities for the medical
school.
For both facilities, users must be trained in the operation of the
instruments before they are given free access (we have coded locks).
During training, people set up appointments with me. Once "cleared fro
soloing" they are given an idivualized code and are then free to reserve
the instruments by means of a weekly sign-up sheet. For now, the sheet for
the following week is posted on Thursday or Friday. Our in-house computing
people keep promising us an on-line sign up. I can't wait since the 2
facil/lab group may reserve on a given day.
This has worked well for us so far. It also seems to satisfy the granting
instituions.
For more details, you can check out the school's web site
(http://www.med.cornell.edu/research/cores/index.html)select either
electron microscopy or optical microscopy to get the operating procedures.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Thu Mar 16 17:42:26 2000



From: Andrew Coutermarsh :      acoutermarsh-at-omegafilters.com
Date: Thu, 16 Mar 2000 11:21:55 -0500
Subject: JOB POSTING

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FOR ALL INTERESTED PARTIES:

Company: Omega Optical, Inc.

Location: Brattleboro, Vermont

Company
Description: 30-year old designer and manufacturer of thin film optical
interference filters used world-wide for laboratory and scientific
equipment.

Position: Fluorescence Product Manager

Position
Description: The Fluorescence Product Manager is responsible for any and
all activities which help further the companyâs sales of fluorescence
products. These activities include:

1. Technical sales and sales support: Technical sales and assistance to
sales force regarding fluorescence applications.
2. Marketing: Marketing activities related to fluorescence products.
3. Product & Market Development: Development activities related to new
fluorescence products, applications, and developing markets.

Omega is seeking an individual who is excited about working with varied
applications of fluorescence detection in the life sciences. The work
involves discussing the best match between laboratory experiments and
Omegaâs wide range of light filters for this science. Experience in
fluorescence, microscopy, life sciences and instrumentation is
essential.

Salary: Competitive with full benefit package.

Contact
Information: Email response with attached Word file resume and salary
history to: acoutermarsh-at-omegafilters.com or snailmail response to:


Andrew Coutermarsh, SPHR, HR Director
Omega Optical, Inc.
PO Box 573
Brattleboro, VT 05302
==============================================================




From daemon Thu Mar 16 17:42:27 2000



From: John Shields :      jpshield-at-arches.uga.edu
Date: Thu, 16 Mar 2000 11:34:59 -0500 (Eastern Standard Time)
Subject: references on optimal animal tissue fix

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Hello everyone,
We are having to justify using cervical dislocation without
anesthesia on a mouse prior to removing the liver for fixation for
our EM class.
Does anyone have a reference on hand that we can cite to show that
direct cervical dislocation is better (or not) for best preservation
of tissue under the circumstances? We won't be doing perfusion,
which would obviously provide better fix.
Please respond directly to me at: jpshield-at-arches.uga.edu

Background:
The class is held once a year, and one mouse is used (usually an
extra, "too old" mouse from the monoclonal facility).
The animal-use officer wants us to hike down to another building with
the class and use CO2 anesthesia, prior to euthanasia, so the mouse
suffers less (?). Then hike back up the hill with the tissue in
fixative. We've never had a problem with our animal-use request until
this year and the guy is busting our chops.

Thanks in advance,
john
********************************************
John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602-2403
(706)542-4080
jpshield-at-arches.uga.edu




From daemon Thu Mar 16 17:42:28 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Thu, 16 Mar 2000 08:45:23 -0800
Subject: Re: Multi-user facility managers

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Hi Leslie
We are a multi-user facility, used by the Faculty of Medicine and the
Faculty of Science. Last fiscal year we had 653 individual users, the
majority frequent users. We have TEM, SEM, confocal,
fluorescent/brightfield, darkrooms and digital imaging equipment.

We use the first-come, first-served approach for many years and so far
there hasn't been a problem with sign-up conflicts with the instruments.
Most people around here are reasonable, thinking people.

We do not give open access after hours (9-5). But if a user is experienced
with the instrument, and I know he/she is not going to mess it up for the
next user, and he/she will take a responsibility lecture from me, there is
a floating key. I used to be more lax with letting users in after hours,
but after a week of just trouble shooting, I got tough. Since then, it has
worked well. I remember when I did my PhD, how much more work I got done
when there were no interuptions or distractions. So I have a sympathy for
allowing responsible researchers in when I am not there, especially as this
is a busy facility during the week day.

If it ever came to a conflict of access to the instruments, I am in charge.
I would think poorly of researchers who didn't play by the rules. The only
time, any problem has occured has been when someone has a crutial deadline.
Then when the situation has been put to the person who has it booked, they
can usually see themselves in that situation and have always been
accommodating. Maybe it is the Canadian way, but I should think it is far
more universal!

Good luck with your grant. Let me know if you need more information.
Elaine

}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Thu Mar 16 17:42:29 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 16 Mar 2000 09:07:14 -0800
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
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At 08:22 AM 3/16/00 -0500, Leslie Eibest wrote:
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}


Leslie,

I have been managing a multi-user facility since 1979. First it was sign
up sheets. Now it is a web-based calendar. Have a look at out web site.
This works fine for us. We use the web calendar because it is easier for
people across campus to sign up this way than to trek over to the facility.
Also, I don't have to answer the phone to make their appointments.


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Thu Mar 16 17:42:30 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 16 Mar 2000 09:49:32 -0800
Subject: RE: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jose,
One other method that might work for you is the old "window-polishing"
technique. This was the preferred method before the jet-polishing machines
were in wide use. A sheet of the metal about one inch by two inches and as
thin as possible is masked by stopping lacquer on the edges and
electropolished in a bath of electrolyte. When it has polished down to the
"lacey" point, a small protrusion of the metal is sliced off with a razor
blade and sandwiched in a folding grid or between two grids. The edges,
except for the sliced part, are thin enough to examine. It is a bit of an
art and takes some practice to get right, but the resulting piece is small
and securely held.
-----Original Message-----
} From: Jose Maria Manero [mailto:manero-at-cmem.upc.es]
} To: Microscopy-at-sparc5.microscopy.com
} Subject: [TEM] Problem with magnetic samples

} Dear all,

} I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
} to study the dislocations substructure. But I have some problems, for
} example, I can not focus the image due to the magnetism of the ferrite.

} I am looking for suggestions to avoid this problem. What could I do?

} Thank you in advance.

} Dr. J. M. Manero
} Universidad PolitŽcnica de Catalunya.
} E.T.S.I. Industriales de Barcelona. Spain.
} e-mail: manero-at-cmem.upc.es

Regards,
Mary


Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Mar 16 17:42:32 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Thu, 16 Mar 2000 11:42:44 -0700
Subject: RE: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie,
You can post a calendar on your internal web page for users to log onto, or
if you do not have a web page post a calendar at each microscope. Let your
users sign up as far in advance as they can, this eliminates most of the
conflicts and the habitual "last minute guy" learns to get him/herself more
organized. It also gives you a record of use, I also log in all down time
due to maintenance service etc. The best part is this system is really easy
once everyone buys into it.
Good luck!
} ----------
} From: Leslie Eibest
} Sent: Thursday, March 16, 2000 5:22 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Multi-user facility managers
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University
}
}



From daemon Thu Mar 16 17:42:32 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Thu, 16 Mar 2000 10:42:29 -0800
Subject: Multi-user facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have modified the basic sign up sheet due to very heavy demand on our
microscope. Except for special cases (eg., time lapse studies, emergencies)
we allow users to sign up for no more than two hour blocks of time and they
cannot sign up for additional time until that block is completed. In
addition, if they are more than 15 minutes late for their time the entire
block of time is forfeited. (This has rarely happened but emphasizes the
need for responsible behaviour.) This system was adapted from the one that
Mei Li Wong uses for electron microscopes.
Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Thu Mar 16 17:42:33 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Thu, 16 Mar 2000 13:03:32 -0600
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Leslie,
Yikes! this reeks of infectious administratium but since you need to deal
with the request...
I have quite a few systems that are considered multiple user
facilities. Over the last 9 years I can say I have never had a conflict to
resolve or even heard about one... meaning the users work well together.
Users make reservations on a calendar. That is their time. If the
machine is down, that time is lost. There is not a ripple effect or
compression of the schedule to squeeze them in Frankly if any user asked
another for time due to there state of desperation I have no doubt that they
would work together.
I have a rule for my EMs that only one 4 hour period may be reserved at
a time. I think this keeps the level of respect for someone else's time
high.
My good fortune aside, you could probably get an outline for conflict
resolution from your HR dept. & append it to your proposal.

Bruce Brinson,
Rice U.

Leslie Eibest wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University



From daemon Thu Mar 16 17:42:35 2000



From: Anastasia Kanellopoulos :      sia-at-sciencewares.com
Date: Thu, 16 Mar 2000 14:45:17 -0500
Subject: SEM/TEM parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings -

We are working on a research project and we need an electron gun with 5-10
kV (wehneldt assembly) and a lens system (for electrons, not photons) that
is typically found in SEM systems. Does anyone have a decommissioned or
un-needed SEM or TEM equipment from which we could get these components at
low or no cost (we are on a very tight budget)? We will even move out a
complete system if it will help someone who needs the space.

Thanks in advance for any help on this!

Sia Karplus
sia-at-sciencewares.com




From daemon Thu Mar 16 17:42:36 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Thu, 16 Mar 2000 11:54:12 -0800
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

I recently responded to a thread about Amray FE maintenance contracts.
My response contained two errors: one numerical, one in perception.
I wish to correct them at this time.

First the numerical correction: I stated that the JEOL FE gun exchange
cost was $17,000.00: it is actually $7,000.00.
The $17,000.00 figure was obtained from two JEOL customers which I
presumed to be true as
I am not in the habit to ask other competitors their pricing schedules.

Second the perception error: I stated that " Hitachi is doing well and
doesn't need to worry about "locking" third party maintenance or anyone
else out."
I did not mean to imply that JEOL or any other manufacturer's business
was poor -- just that Hitachi was doing well. I am sure that JEOL's
business is excellent as they have an excellent product.
Moreover, their service organization is one of the best. As their
competitor we have a difficult time competing with JEOL.

Keep in mind that I do not have a "cozy" relationship with Hitachi. I
just respect the technology & the professional co-operation I get from
them & other manufacturer's like them.
I can buy schematics, parts lists, & even service manuals from Hitachi
that other manufacturers don't offer because they consider it
proprietary.
It makes my job easier.

I was using Hitachi as one example because in a pure free enterprise
system the customer is the ultimate winner.

I apologize for the errors. I wish to thank JEOL for pointing out the
error and their gracious attitude in dealing with this error.

Regards,

Earl Weltmer
Scanservice Corporation



From daemon Thu Mar 16 17:42:36 2000



From: Diana_Papoulias-at-usgs.gov (Diana Papoulias)
Date: Thu, 16 Mar 2000 13:52:58 -0700
Subject: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could someone please tell me where I can purchase accu-edge microtome
blades?

Thank you.

Diana Papoulias
USGS
4200 New Haven Rd
Columbia, MO 65201

T:573 876 1902
F:573 876 1876
E:Diana_Papoulias-at-usgs.gov



From daemon Thu Mar 16 17:42:38 2000



From: Ron L'Herault :      lherault-at-bu.edu
Date: Thu, 16 Mar 2000 15:36:09 -0500
Subject: RE: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a calendar that people fill in. As a courtesy to others, we ask
that they not block more than 4 hours at a time. So far this has worked
well. On the few occasions when another user was in a bind, scheduled
users have been accommodating because they know that they could be in the
same position.

Ron

-----Original Message-----
} From: Leslie Eibest [SMTP:leibest-at-duke.edu]
[} ]
[} ]
I would greatly appreciate insights from managers of
multi-user facilities of any sort as to your approaches to allocation
of access to instrumentation, and methods of resolving conflicts
among researchers.





From daemon Thu Mar 16 17:42:44 2000



From: flaitz-at-us.ibm.com
Date: March 29, 2000
Subject: Meeting: Metropolitan Microscopy Society, Fishkill, NY.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Metropolitan Microscopy Society

Spring Meeting 2000


Time: 8:45 am (registration begins)

Place: IBM Microelectronics, E. Fishkill, NY, West Complex, Bldg 600.

The IBM Corporation has graciously agreed to be our host for this
meeting at their facility in East Fishkill, NY (West Complex, Bldg
600). Attendees will be able to purchase lunch in the adjacent IBM
cafeteria. Due to size and security issues IT IS ESSENTIAL THAT
MEMBERS PRE-REGISTER so that an attendee list can be delivered to the
IBM security folks to prepare guest badges and escorts. DUE TO THE
TIGHT SECURITY CLEARANCE REQUIREMENTS, WALK-INS CANNOT BE
ACCOMMODATED.

THE REGISTRATION DEADLINE IS MARCH 24th AND CAN BE ACCOMPLISHED
ELECTRONICALLY. Please respond via email (or fax) to Evan Slow
directly. A simple email note or a completed fax of the registration
form is all that s required to register. You can then bring the
required fee with you to the meeting. The meeting fee, which does not
include lunch, is $15.00. ON-SITE REGISTRANTS WILL BE CHARGED $25.00
BUT, AGAIN, UNLESS YOU HAVE BEEN PREVIOUSLY CLEARED THROUGH IBM
SECURITY, WE CANNOT ACCOMMODATE YOU.

Registration: Email -- ess-at-feico.com
FAX --- (201) 760-2525

----------------------------------------------------------------------

AGENDA


8:45 - 9:30 : Registration (Coffee)


9:30 - 9:45 : Introductory Remarks (Al Sicignano).


9:45 - 10:45 : CHARACTERIZATION OF MICROSTRUCTURES IN MICROELECTRONIC
INTERCONNECTS, Lynne Gignac, IBM T.J. Watson Research Center.


10:45 - 11:45 : IMAGING, DIFFRACTION AND SPECTROSCOPY WITH FIELD EMISSION
GUN SEM (FEG SEM) AT LOW VOLTAGE, Vinayak Dravid, Northwestern U.


11:45 - 12:45 : DEEP-UV CONFOCAL MICROSCOPY OF SUB-MICRON FEATURES, Mike
Torres, Metron Technology.


12:45 - 1:30 : LUNCH -- available for purchase in the IBM cafeteria.


1:30 - 2:30 : THE USE OF MONTE CARLO CALCULATIONS FOR QUANTITATIVE X-RAY
MICROANALYSIS, Eric Lifshin, GE Corporate Research & Development.


2:30 3:30 : STUDIES OF SAMPLES HAVING SHALLOW SURFACE TOPOGRAPHY BY
THE LOW-LOSS ELECTRON (LLE) METHOD IN THE SCANNING ELECTRON MICROSCOPE
(SEM), Oliver Wells, IBM T. J. Watson Research Center.







From daemon Thu Mar 16 18:10:43 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 17 Mar 2000 10:55:04 +1100
Subject: Re: Multi-user facility managers

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At 08:22 16/03/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have run a first come-first served policy for 30 years. Its the way to go.

It now is self-administering through our equipment booking software.

VERY seldom we need to reason with excessive users. We can almost always
sort problems out with time trading.

Use Internet explorer - Go to http://srv.emunit.unsw.edu.au/ - instrument
booking and use login {guest} password {guest} to get its flavour.


}
}
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Thu Mar 16 18:30:49 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 16 Mar 2000 18:27:29 -0600
Subject: Multi-user management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It helps to have a good management plan in place whether the facility is extremely busy or not. It avoids most conflicts and gives manager/coordinator a set of rules to use that can be applied to any situation that might pop up. You might not that busy currently on conflicts are occuring but later on the situation can quickly change. Granting agencies want to know if the equipment they are funding are accessible and not misused. We are a fairly busy facility and have been using calendars, lined up together, on a wall for each piece of equipment including work stations. We have one for the current week and below that one for the next week users can sign up. Depending on the instrument from two to three hour slots at a time during peak hours. After 6PM and before 8:30AM longer durations are possible. One can only cancel a day in advance on an instrument. No shows are charged. We cross out time slots for instrument maintenance as needed and as much inadvance as possible. Potential users first fill out an investigator profile form before they can cycle into the facility. This helps us target the instrument/technique they need. Based on several factors we prioritize users. All users must take a tutorial and demonstrate competency before they can solo. We do tutorials only one day a week unless there is an important reason that they cannot do it on that day. In a few weeks, I hope, we are going to our web based scheduler in which users have the a couple months to schedule in advance. It will automatically restrict a users' time usage to only peak hours or to peak and after hours depending on their competency, and other criteria. It will prevent users from making cancellations less then the day before. Down times, etc can be easily entered on the calender. The web based schedular will make it allot more convenient for users and core staff. And best of all, please god let it work smoothly, data from the scheduler will flow into our billing data base for semi automatic billing. So far, with the written calendar syst
conflicts, however, usage is so high on some instruments not every user can get the slot they need and so are unhappy campers. In this case, we encourage users, if it is possible, to time their experiments based on when they can get on the instrument.
I hope this helps.

Hank Adams
Lab Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX



From daemon Thu Mar 16 18:30:51 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 17 Mar 2000 10:55:04 +1100
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 08:22 16/03/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have run a first come-first served policy for 30 years. Its the way to go.

It now is self-administering through our equipment booking software.

VERY seldom we need to reason with excessive users. We can almost always
sort problems out with time trading.

Use Internet explorer - Go to http://srv.emunit.unsw.edu.au/ - instrument
booking and use login {guest} password {guest} to get its flavour.


}
}
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Thu Mar 16 20:11:32 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 16 Mar 2000 19:57:01 -0600
Subject: Re: Subject: SEM - Problems with marking bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christian, I have a few approaches you might consider: a. Where
possible, I usually obtain a culture of the bacteria thought to adhere to a
substrate and prepare it exactly the same way:fix, dehydrate, cpd and
sputter-coat. b. Prepare two samples but after full prep, apply a cellulose
acetate film (or duco cement) to one surface moistened with a drop of
acetone and try to strip the bacteria from the bone cement -- I don't know
about     damage to the methacrylate. This technique
produces a replica of the surface.  I have used it to remove
bacteria colonizing a planchet of hornblende.  We were able to locate
the pits made by the bacteria and image the bacteria removed, i.e.,
stripped from the mineral. c. If the above approach fails to produce the
results you need, try making a replica with double sided C tabs, the type
sold by EMS for X-ray microanalysis. d. If staph epidermidis has a
glycocalyx you might try adding ruthenium red to the GA in the primary fix
and prep as usual but instead of coating with Au or Au/Pd, evap C and use a
backscatter detector to obtain a contrast BE image where the brighter
areas should be the Ru stained bacteria and/or collect a spectrum to
check for the presence of Ru.  If there is a characteristic peak, you
could map the Ru and  localize the bacteria with an x-ray dot map. I
would be interested in knowing how you resolved this problem. Rosemary
Walsh The Electron Microscope Facility for the Life Sciences, A Shared
Technology Facility, The Life Sciences Consortium 1 South FrearLab Penn
State University University Park, PA 16802 (814) 865-0212 rw9-at-psu.edu
{"http://www.lsc.psu.edu/stf/em/home.html"
eudora="autourl"} http://www.lsc.psu.edu/stf/em/home.html




From daemon Thu Mar 16 23:05:51 2000



From: Ford Royer :      froyer-at-bitstream.net
Date: Thu, 16 Mar 2000 22:59:16 -0600
Subject: Re: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I sell the DruAedge blades. Same quality as the accu-edge, but at a better
price. Please contact me for pricing and free samples. I carry both the High
Profile and Low Profile Teflon coated blades.

Ford M. Royer
Analytical Instruments, LLC
9921 13th Ave. N.
Minneapolis, MN 55441
phone: (800) 565-1895, ext. 17
fax: (612) 929-1895
froyer-at-bitstream.net

Diana Papoulias wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Could someone please tell me where I can purchase accu-edge microtome
} blades?
}
} Thank you.
}
} Diana Papoulias
} USGS
} 4200 New Haven Rd
} Columbia, MO 65201
}
} T:573 876 1902
} F:573 876 1876
} E:Diana_Papoulias-at-usgs.gov



From daemon Fri Mar 17 06:37:23 2000



From: Molecular Biology Lab - Dept of Botany :      paam-at-rs.uovs.ac.za
Date: Fri, 17 Mar 2000 09:45:10 GMT2
Subject: TEM Immunogold labeling spesificity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to localise a beta-1,3-glucanase gene after Russian wheat aphid
infestation. However I am getting quite heavy labeling in the chloroplasts and
from the literature this haven't been previously documented.
My control serum shows a small amount of labeling in cell walls but these
seems to be random. The controls (before infestation) shows very little
labeling.
Work done with the same antibody on rust infested wheat plants have shown
no, to very little labeling in the chloroplasts.
My question is how can I make sure this is not artifacts and that the glucanase
are for sure in the chloroplasts.
Martin Wilding
Department Botany & Genetics
University of the Orange Free State
P.O. Box 339
Bloemfontein
9300
South Africa

Tel +2751 4012818
Fax +2751 4488772
Email paam-at-rs.uovs.ac.za



From daemon Fri Mar 17 07:47:26 2000



From: Tom Isabell :      tc_isabell-at-fischione.com
Date: Fri, 17 Mar 2000 08:29:01 -0500
Subject: Open Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


E.A. Fischione Instruments, Inc. is seeking an individual for the position
of a Sales Engineer.
The successful candidate will be responsible for one or more states from
both inside the office in Export, PA. and in the field. The candidate's
responsibilities will include defining contacts, maintaining information in
the sales database, qualifying leads and determining need, providing
information and quotations for standard products, handling service/spare
parts requests and coordinating the same with the Service Department. Must
develop a thorough technical knowledge of standard products. Extensive
travel required.

The candidate should have an Associates Degree in either Electron
Microscopy technology, Material Science, Engineering, or Physics as a
minimum; a B.S. or M.S. would be preferred.

Salary is commensurate with experience.

E.A. Fischione Instruments, Inc. is an equal opportunity employer. Please
send your resume and salary requirements to:

Human Resources Director, MSA LS
E.A. Fischione Instruments, Inc.
9003 Corporate Circle Export, PA 15632
Phone (724) 325-5444 FAX (724) 325-5443
E-mail: info-at-fischione.com
www.fischione.com



From daemon Fri Mar 17 07:57:26 2000



From: Louis M Kerr :      lkerr-at-mbl.edu
Date: Fri, 17 Mar 2000 08:53:52 -0500
Subject: Re: LM: Fluorescence lamp instability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David and others,

This does not directly address the problem with your current lamp but you
might want to consider looking at this new lamp assembly. We recently
evaluated the system and there were no visible stability problems as well
as several advantages over the standard mercury lamp housing.

The company is called EFOS. This unit replaces the standard mercury source
for fluorescence and possibly transmitted light techniques. It uses an
external 50W miniature arc lamp incorporated into an elliptical reflector
which is connected to the microscope by a liquid light guide. Our unit was
mounted on a Zeiss Axioscope.

You can visit the EFOS web site at http://www.efos.com/products/x-cite.htm

Thanks,
Louie


At 2:56 PM -0400 3/15/00, O'Neil, David wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu




From daemon Fri Mar 17 17:05:07 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 17 Mar 2000 10:46:12 -0500
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie Eibest wrote:

} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}

Dear Leslie,
Our facility is funded by NIH as a Biotechnological
Resource, so we deal with both in-house and outside researchers.
Like the other responders, we have not had any conflicts between
users since we started. Because the outside users must spend con-
siderable time arranging to travel here to use the instruments, we
have had a policy which maximizes the utility of their stay here.
We have a staff person whose function is outside user liason;
she ascertains what the user wants, suggests ways to prepare the
specimen to achieve that, examines trial grids, schedules the user,
and stays with the user during the run. For the inexperienced
user, she does almost everything except select the area of the grid
to be photographed; for users who are familiar with our facility,
she changes specimens, develops film, and is available for any
other user needs. For the occasional user who can operate the
HVEM on his own, she still changes the film and is available.
The rest of the staff is responsible for starting the HVEM
up in the mode the user wants, taking care of any problems which
arise, and setting up for non-routine use--EDS, diffraction, etc.
In-house users can be bumped to accomodate outside
users and can only sign up a few weeks in advance. Outside users
can sign up for as far in advance as they wish, and if any problems
arise with the scope, our liason person calls to let them know so
they can change plans if necessary. In periods of very high use,
the staff arranges to keep the scope up for as long as 16 hours a
day and will also arrange to be here after hours and/or on week-
ends to accomodate users' schedules.
Yours,
Bill Tivol



From daemon Fri Mar 17 17:05:07 2000



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Fri, 17 Mar 2000 08:31:11 -0800 (PST)
Subject: capture speed for image ratios

Contents Retrieved from Microscopy Listserver Archives
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Hello microscopist,

Is there a simple answer to the question:

How fast must the sequential image capture be to perform image ratios for
dtermining something like calcium ion levels? If you have a fast interline
or frame transfer camera, does a single filter wheel like a Ludl change
filters fast enough?

Bob
Morphology Core Lab
U of Washington
Seattle



From daemon Fri Mar 17 17:05:08 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Fri, 17 Mar 2000 11:57:49 -0500
Subject: Job announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cell Biologist/Microscopist

SmithKline Beecham, a world class leader in Research and Development, continues
to pioneer innovative pharmaceutical and healthcare products and services. We
have the following opportunity available at our state-of-the-art suburban
Philadelphia facility. Working in our Safety Assessment department you will
provide technical support and scientific input into the design and execution of
studies to elucidate the cellular mechanisms of drug-induced toxicities. You
will prepare biological specimens for transmission and scanning electron
microscopy, confocal microscopy, X-ray microanalysis and immunohistochemistry.
You will also perform qualitative and quantitative data analysis using computer
assisted image analysis systems. We require a BS/MS in a biological science, and
3-5 years of microscopy experience in cell biology, physiology, toxicology.
SmithKline Beecham is dedicated to an innovative workplace and supports you with
career long opportunities and learning. We offer a competitive benefits and
compensation package. For confidential consideration, please forward your
scannable resume to:
SmithKline Beecham
Attention: Human Resources
AD CODE: 2K0325W
c/o National Resume Processing
P.O. Box 1070
Burlington, MA 01803 USA

Indicating ad code is essential. Principals only, no agencies, please. For a
full listing of current opportunities, or to submit a resume online, visit our
website at www.sb.com/careers.

**********************************************************************************************

Please respond directly to the address in the advertisement, and not to me.

Regards,
Bev Maleeff




From daemon Fri Mar 17 17:05:08 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Fri, 17 Mar 2000 12:03:46 -0500
Subject: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie,
Just to add my two cents: I supervise a private Photopathology Lab for the
Wellman Labs of Photomedicine at MGH. We have an Axiophot (Zeiss)
photomicroscope that has an image analysis system attached. We have used a
monthly sign-up calendar successfully for 10 years. The room is secured--
anyone wishing to use the system, must be checked out first by one of us.
Once we are con-
fident they know what they are doing, they then sign up ahead for a day and
time. We allow after- hour use; many researchers work weekends and nights.
I sign out a lab key to the individual and the room key is in a desk
drawer. People are instructed to make sure the microscope is off, doors
are locked, lights are off and room is secure. Other than the occasional
light left on, the system has worked. The other advantage to having
people sign-up is that if there is a problem with the micro- scope and/or
room, we can go back to the last person who used it and advise them of the
problem.
We also use a sign-up system for a multi-headed teaching scope.

Whatever system you end up using--sign-up sheets or on-line, you have a
record of use, which granting agencies are most interested in: making sure
you get the most for your buck!

Good luck!

Peggy Sherwood
Wellman Labs of Photomedicine-MGH
50 Blossom Street
Boston, MA 02114
617-726-6983 (Photopathology Lab)
617-724-4839 (voice mail)
617-726-3192 (fax)
e-mail: sherwood-at-helix.mgh.harvard.edu






From daemon Fri Mar 17 17:05:08 2000



From: Leslie Eibest :      leibest-at-duke.edu
Date: Fri, 17 Mar 2000 12:23:33 -0500
Subject: Multi-user facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks so much to all of you who took the time to respond to
my question. My management techniques are basically the same as most
of yours, but my presentation was woefully inadequate. Thanks to
you, I'm well-armed now!

Leslie Eibest



From daemon Fri Mar 17 17:05:09 2000



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 17 Mar 2000 10:57:39 -0700
Subject: Multi-user facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had several instruments and many instrument users during each the
last twenty five years. Originally, we used calendar sign-up and log book
entries to compile data for accounting and annual reports. This became too
time-consuming so we developed an in-house automated (computer-based)
sign-up (registration) system in 1990. The system serves as an instrument
reservation unit and an automated accounting system. We recently developed
(continue to develop) a web-based system which allows researchers to
reserve time from their offices or anywhere they can access the internet.
You may wish to view it at the following URL:
http://signu.la.asu.edu:8001/. (Login: guest Password: testit) You will
not be able to view the accounting data. The sign-up program allows only
qualified users to operate an instrument. Also, rules for successive time
reservation are integrated into the program. Optional holders and
spectrometers can be reserved at the time the instrument is signed for.

We presently have 90 research users. In addition, there are approximately
sixty student users (grad and undergraduates) who are taking semester
classes in microscopy or they have one or two sessions on individual
instruments to better understand the role of electron microscopy for
solving problems in Geology, solid state chemistry etc.


Each instrument has a keypad timer interfaced to either the filament or
high voltage on/off switch, depending on ease of accessibility. The timer
is interfaced to a 486 computer (obtained from surplus-there are lots of
these available). The computer stores user time for each account as well
as the number of films exposed for the instrument used. Because we have to
pay for approximately 90% of our operations costs, we have to charge for
use of the instruments. We also charge different hourly rates depending on
user status (inside or outside user).

At the end of each month, the user time/film number data is dumped into
another computer which compares the reserved time from the web-based
reservation system to the actual use time and charges for the greater of
the two. If the instrument develops problems during the use time and can't
be used any more, the individual user is able to send a DOWN message to the
reservation computer and the user is charged only for actual time used. The
monthly use data is formatted into a spreadsheet which contains microscope
use times and film numbers used. A principal investigator (PI) may have
ten grad students who use five different instruments and three different
grant account numbers during the month. He/she receives a statement at the
end of the month which identifies the microscope user by name, the account
number used, the hours used (rounded to the nearest minute)for each
instrument and the total number of films exposed for each use time. The
total costs for instrument use is given on the last line of the statement.

An advantage of this system is that within thirty minutes, one can sort
data files and determine how many hours each microscope is used over any
period of time; how many hours each user utilizes an instrument(s) over
time; number of hours used for each account and total number of hours for
all instruments over time. This info is useful for reports to
administrators as well as for funding agency applications.

If you have questions about our system, please e-mail me directly or call
at the number listed below.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Mar 17 17:05:11 2000



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 17 Mar 2000 13:34:08 -0700
Subject: Multi-user facility--correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I forgot to put the p in signup for the URL in the message I sent out this
morning. Thanks to David Kenriks for catching it. The right URL is:

http://signup.la.asu.edu:8001/

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Mar 17 17:05:11 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 17 Mar 2000 15:33:12 -0400
Subject: RCA EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all:

A short time ago there was a discussion about the different EMs RCA
produced and when they were produced. The other day I was digging through
my sfiles and came across a copy of the August 1967 issue of the RCA
Scientific Instruments News which has pictures of all the models produced
and the year of introduction:

EMA 1939 I don't think this model was ever sold commercially.
I believe it was built in the RCA Labs for developmental uses

EMB 1940 This was the first commercial model

EMC 1944 This model had a horizontal column

EMT 1950 This was an inexpensive table model that was designed with
use in high schools and small colleges in mind.. As I recall
it had lenses made of permenant magnets to eliminate the need
for lens power supplies and to hold the cost down.

EMU 1944 This was probably RCA's most popular model. It was on their
leading EM for at least 10 years.

EMD 1948 This was an electron diffraction instrument, designed
specifically for obtaining ED patterns by the reflection from
solid surfaces at a grazing incidence.

EML 1954 This was the forerunner of the popular EM-U# and EM-U4 models,
but provided an accelerating voltage of only 50kV. It was
the model in which RCA pioneered the modern ergonomic layout
of controls, with a desk-like design.

EMU-3 1955 Similar in appearance to the EML, but providing 100 kV.

EMU-4 1966 A refinement of the EMU-3, providing a double condenser lens,
a specimen stage airlock system, etc.

RCA ceased marketing electron microscopes in 1969. I was President of
the EMSA that year and discussed the matter with James Hillier, one of the
pioneers in the field of electron microscopy, and a Vice President of RCA
at that time. He said that it was determined that RCA could make more
money selling records and related electronic devices than EMs, and so was
phasing out of the EM business.

The issue of RCA Scientific Instruments News in question also has a picture
of each model on the cover. I have had a copy run off in jpg format. I
understand that it is not appropriate to transmit such info via this list
server; however, if anyone would like a copy, let me know and I'll send it
to you individually.

Happy St. Patrick's Day,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 17 17:05:11 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 17 Mar 2000 12:51:25 -0600
Subject: SEM maintenance, et. al. (semi-long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 01:54 PM 3/16/00 , you wrote:

} Dear Listservers,
}
} I recently responded to a thread about Amray FE maintenance contracts.
} My response contained two errors: one numerical, one in perception.
} I wish to correct them at this time.

[snipped for brevity sake]

Thanks Earl for the update and correction. This is good info. And
I see some room for additional discussion and inquiry to wrap up this
topic.

The prospects for third party maintenance contracts are rather
variable--depending mostly, I think, on what is being maintained
under contract. This would be based on the availability of schematics,
software, special parts, etc., etc. If some FESEM makers do not
"guard" their FE guns relative to others, then of course, that will weigh
heavily on third party options. For the record, here are some figures
for Amray maintenance contracts. An 1830 LaB6 system runs
$13,500 annually. This excludes "consumables" such as apertures,
LaB6 cathode, pump oil, etc. But it does include on-site service,
troubleshooting, travel, lodging, meals, rental car, things that
break down, like Balzers 240 turbo pump or Edwards E2M8 mechanical
pump, or the invariable broken wire. Actually, I have found the
1830 to be very reliable and a good performer.

The 1910FE runs $15,000 annually. Same terms as the 1830 but
includes the computer control system. What I don't know is whether
Amray will sell the FE gun assemblies to third party maintainers. I do
know that for Amray users who are not under an Amray contract, the
305FE gun costs about $14,000 total to replace as a per-diem item.
Again, I have found that this SEM also is very reliable. But what
about performance?

Performance wise, I suppose we could have an "image shoot off"
contest based on a standard specimen and different SEM
instruments of different models and from different makers. But there
are some basic differences among SEMs that would otherwise
seem to be the same--but are not. Notable among these differences,
for example, is the design and construction of the final lens pole piece. This is
due, I think, to two or three driving forces. One is the need to image physically
large specimens at various WDs. This means that one needs a large chamber
with a stage that offers accommodation of large samples and wide WD
range. The second force is the requirement to image whole semiconductor
wafers ranging in diameters from 4" to 8" and to have a wide travel
distance across the wafer and to operate well at low KV (photo resist examination,
for example). The third force is the impact on the SEM when needing to
do X-ray analysis.

The flat lens design is quite useable for large and small specimens when
high tilt angles do not increase the WD to an unsatisfactory figure. In the
case of IC wafers, the conical lens is required. These are available as 45
or 60 degree designs. Since most semiconductor qualification imaging
is done at either zero or 45 degrees, the 60 degree lens is probably
optimal. What Amray has done in regards to conical lenses is an
evolutionary path. The 1830 has a conical lens to accommodate high
tilt angles when working with wafers. It also can be configured with a
large chamber. However, the design of the lens is such that high
resolution is obtained at 10KV-15KV and above. It is not a high
resolution SEM at low KV. Alternatively, with a 25KV or 30KV power
supply, it has ample voltage to support the beam currents needed
for x-ray work. I have no personal information on what the other
makers have done and currently offer in regards to IC wafer inspection
and analysis.

The Amray 3600LEAP was a major design departure. This system
also has a 60 degree conical lens, a huge chamber but a specially
designed lens and other features key to semiconductor work. The
LEAP lens was designed for low KV imaging. At 2-3KV, the LEAP
is virtually identical in resolution to the other lens types at 15KV
and at the same WD. This is a key feature and requirement for
imaging fine pitch and small feature size wafers and photoresist.
The other important feature of the 3600LEAP SEM is the 5-place
heated final aperture holder. Since the 3600 runs with 35u and
70u apertures (or less) as routine, these Pt apertures are
continuously heated to keep the effects of contamination
(photo resist again) at a minimum. Not only does this keep the
apertures clean, but it also prevents accumulation of PR in the
scan coil liner. This FESEM is a very capable instrument from
my experience with it. It can easily image 0.15u wide gate poly
at high resolution and 2.5KV at WD=6mm.

If one considers where I have wound up with this discussion,
it is cause for pause to ask a fundamental question. That is, "What
is a research SEM?" Different applications must surely lead
one to select one type of instrument over another. Does it also
direct a path to one manufacturer over another? Since I have
not seen the myriad number of SEM models from all of the vendors,
I cannot answer this question. But while there might be some
disdain for one maker versus others, it seems tough for me to
make any sort of quantitative decision when there are quite a
few variables involved--solely based on application, much less
vendors and maintenance options.

What do you think about this, based on your experience?

gary g.









From daemon Fri Mar 17 17:05:12 2000



From: Nathan Haese :      nathan_haese-at-CompuServe.COM
Date: Fri, 17 Mar 2000 17:22:00 -0500
Subject: SAS Dinner Mtg 4/12 in Mountain View, CA on Raman of Polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TOUR SPEAKER (Society for Applied Spectroscopy) - 4/12/2000 Meeting in
Mountain View

"RAMAN SPECTROSCOPY AS A PROBE OF STRUCTURE/ORIENTATION IN POLYMERIC
FIBERS"

by DR. BRUCE CHASE (DuPont Central Research, Wilmington DE)

at Michaels at Shoreline, Mountain View (Directions to follow)

Register 6:45 PM
Dinner 7:30 PM
Talk 8:30 PM

Cost: $30 for dinner ($10 students and teachers), free for talk.

Dinner Choices: (Indicate Choice when RSVP'ing)

Breast of Chicken, Piccata
Chilean Sea Bass, Ginger Shallot Sauce
Grilled Vegetable Brochette with Wild Rice

Reserve by April 10 to Steve Rabin, 650-564-5315 or steve.rabin-at-alza.com

(Please let us know if you're coming for dinner, or just the talk for
headcount purposes)

Also, see our web site at www.sas-ncss.org

ABSTRACT
Polymeric macroscopic properties such as tenacity, modulus, elasticity,
etc. are determined by molecular level effects such as conformational state
populations, orientation, and intermolecular interactions. Significant
changes occur at the molecular level as a molten or solution phase polymer
is spun into fiber form. The polymer goes from an unoriented, amorphous
state to an oriented, semi-crystalline material via the deformations
imposed by spinning and drawing. Raman spectroscopy can potentially
provide information on both structure and orientation. Changes in band
intensities can be related to conformational populations and to formation
of crystalline regions. Changes in relative intensities as a function of
incident and scattered polarization yields information on chain
orientation. The use of polarized Raman scattering done in both 90 and 180
degree scattering geometries has been used to determine the second and
fourth order orientation functions for both polyethylene and PET fibers.
Raman measurements have been made in both the laboratory frame and on-line
for 66 nylon and PET fibers. For both PET and 66 nylon, crystallinity
levels and a qualitative measure of orientation can be obtained.

DIRECTIONS to Michaels (in Shoreline Park, Mountain View):

} From Anywhere in the Bay Area:

Get to Highway 101 toward Mountain View (from SF and the Peninsula,
southbound; from San Jose and the East Bay via 237, northbound)

Go to the Shoreline Rd. exit and turn left at the end of the exit road.
Follow Shoreline Rd. into (approx 1 mile) through Mountain View Park gate.
Continue on the single lane road (golf course on your left) for approx 1
mile, turn left at Michaels restaurant sign into the parking lot.



From daemon Sat Mar 18 08:09:09 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 17 Mar 2000 18:57:06 -0600
Subject: RE: Fluorescence lamp instability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following up on the Hg burner problems, does anyone have
a schematic of the "transformer" for these? There is assumed
to be a true transformer plus a rectifier and filter. Without
a circuit diagram, there is too much speculation for me about
what is actually going on. It seems that there are some common
problems out there. These ought to be readily solvable.

It really cannot be that difficult, can it? Maybe so.

gary g.



From daemon Sat Mar 18 08:09:10 2000



From: Jeff & Wanda Gray :      jefwan-at-flash.net
Date: Fri, 17 Mar 2000 21:12:49 -0600
Subject: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Diana,
These blades are available from Allegiance Scientific (used to be Baxter,
used to be Scientific Products, used to be....). As far as I know, this is
the only place that carries this brand.
I have no financial interest in Allegiance, just passing along a fact.
Wanda Shotsberger
(HT ASCP)

-----Original Message-----
} From: Diana Papoulias [mailto:Diana_Papoulias-at-usgs.gov]
Sent: Thursday, March 16, 2000 2:53 PM
To: microscopy-at-sparc5.microscopy.com


Could someone please tell me where I can purchase accu-edge microtome
blades?

Thank you.

Diana Papoulias
USGS
4200 New Haven Rd
Columbia, MO 65201

T:573 876 1902
F:573 876 1876
E:Diana_Papoulias-at-usgs.gov




From daemon Sat Mar 18 08:09:12 2000



From: COURYHOUSE-at-aol.com
Date: Fri, 17 Mar 2000 23:35:44 EST
Subject: Re: RCA EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to get hold of an RCA model EMT for display for our museum
collection.
Ed Sharpe archivist for SMECC


{ { Subj: RCA EMs
Date: 3/17/00 2:47:16 PM Pacific Standard Time
From: bigelow-at-engin.umich.edu (Wil Bigelow)
To: AAmy-at-dtsc.ca.gov, JRowe6427-at-aol.com, cgarber-at-2spi.com (Chuck Garber),
john.mardinly-at-intel.com, microscopy-at-sparc5.microscopy.com (Microscopy
Listserver), oshel-at-shout.net

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Hi all:

A short time ago there was a discussion about the different EMs RCA
produced and when they were produced. The other day I was digging through
my sfiles and came across a copy of the August 1967 issue of the RCA
Scientific Instruments News which has pictures of all the models produced
and the year of introduction:

EMA 1939 I don't think this model was ever sold commercially.
I believe it was built in the RCA Labs for developmental uses

EMB 1940 This was the first commercial model

EMC 1944 This model had a horizontal column

EMT 1950 This was an inexpensive table model that was designed with
use in high schools and small colleges in mind.. As I recall
it had lenses made of permenant magnets to eliminate the need
for lens power supplies and to hold the cost down.

EMU 1944 This was probably RCA's most popular model. It was on their
leading EM for at least 10 years.

EMD 1948 This was an electron diffraction instrument, designed
specifically for obtaining ED patterns by the reflection from
solid surfaces at a grazing incidence.

EML 1954 This was the forerunner of the popular EM-U# and EM-U4 models,
but provided an accelerating voltage of only 50kV. It was
the model in which RCA pioneered the modern ergonomic layout
of controls, with a desk-like design.

EMU-3 1955 Similar in appearance to the EML, but providing 100 kV.

EMU-4 1966 A refinement of the EMU-3, providing a double condenser lens,
a specimen stage airlock system, etc.

RCA ceased marketing electron microscopes in 1969. I was President of
the EMSA that year and discussed the matter with James Hillier, one of the
pioneers in the field of electron microscopy, and a Vice President of RCA
at that time. He said that it was determined that RCA could make more
money selling records and related electronic devices than EMs, and so was
phasing out of the EM business.

The issue of RCA Scientific Instruments News in question also has a picture
of each model on the cover. I have had a copy run off in jpg format. I
understand that it is not appropriate to transmit such info via this list
server; however, if anyone would like a copy, let me know and I'll send it
to you individually.

Happy St. Patrick's Day,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237

} }



From daemon Sat Mar 18 08:09:16 2000



From: mangesh bagade :      mbagade-at-usa.net
Date: 18 Mar 00 02:36:54 MST
Subject: Information of deposition study of chromium on tungsten.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear microscopist,
Kindly give me information and websites available on adsorption, desorption
and diffusion study of Chromium on Tungsten by field emission and field ion
microscopy. Also nucleation and growth of Chromium on Tungsten.
Thanking you,




Mangesh Bagade
Govt College Of Engineering Pune
Maharashtra, India.

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1



From daemon Sat Mar 18 08:09:17 2000



From: mangesh bagade :      mbagade-at-usa.net
Date: 18 Mar 00 02:37:37 MST
Subject: Information of deposition study of chromium on tungsten.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id DAA13628
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Dear microscopist,
Kindly give me information and websites available on adsorption, desorption
and diffusion study of Chromium on Tungsten by field emission and field ion
microscopy. Also nucleation and growth of Chromium on Tungsten.
Thanking you,




Mangesh Bagade
Govt College Of Engineering Pune
Maharashtra, India.

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1



From daemon Sat Mar 18 14:38:50 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Sat, 18 Mar 2000 11:33:48 -0800
Subject: SEM maintenance, et. al. (this one is long)

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H i Gary,

Your e-mail poses some interesting question & perspectives.

As far as the variation of Third Party maintenance contracts,
Scanservice generally offers contracts that are exactly the same as the
given manufacturer.
The customer can then compare "apples against apples". Whenever there is
a question regarding coverage we always look to see what the
manufacturer's contract covers. The variation in contracts is only
between the different manufacturer's. Cambridge (LEO) did not cover
scan coils, ETEC did not cover rotary pumps or CRTs, etc. This
eliminates the confusion for our customers. We can and do offer modified
contracts where the customer is responsible for parts or a limited
number of service calls but only at the customer's request.

All manufacturer's , SEM & otherwise, are required by Federal Law to
sell parts to third party organizations. To withhold parts is considered
a "restriction of trade" and has some hefty consequences. I have had
accounts set-up for just about all the manufacturers. It is relatively
easy process & most manufacturer's treat us like any other customer in
need of parts.

The only exception is Amray that has a multi-step process for obtaining
parts which is as follows:

1. Request part number.
2. Wait two to three days for part number.
3. Send credit references.
4. Wait two to three days for response.
5. Send banking references & information.
6.Wait two to three days for response.

The above process has occurred at least three times in the last three
years. Finally, I requested that they send the parts COD or I could
prepay.
The response I got was that I "could have done that all along."

Bottom line: manufacturer's must sell to Third party organizations, most
are very professional & co-operative.

As far as an "image shoot-off", I really don' think that would be very
practical as there are significant variations even between the same
model from a given manufacturer. I would expect the best performing SEM
from each manufacturer involved, rather than an average of the industry.

When I worked at ETEC, we noticed differences between the different SEM
columns. The demo unit always got the best column. Even today I
recommend that customers purchase the "demo unit" as it probably has the
best of everything & is tuned up. Moreover the "demo unit" can be sold
at a lesser price as it is technically used. I also recommend that
customers considering an SEM purchase use the model they are considering
at another customer's site. In this way, the potential customer can
evaluate the SEM model on their own terms using their own standards and
without the applications engineer's influence. Most manucfacturer's want
to use the standard "gold on carbon" to highlight their given SEM. I
generally do not see many of my customers imaging "gold on carbon" on a
daily basis. Customers has such a variety of samples: photo resist,
uncoated teflon, etc.

This reminds me of a customer at Hughes who I recommended that she
follow the above procedure of evaluating the SEM at a customer site &
requesting that the demo unit be sold to her Company. She did neither.
When she requested that the demo unit be sold to her Company, the
manufacturer declined. No reason was given. She bought the FESEM anyway.
After about three months I inquired about the status of her new FESEM.
Instead of excitement she responded, "Oh it's OK. I guess I will just
have to get used to it". She continued to use her standard tungsten SEM
over the FESEM.

As far as your analysis of the SEM chambers & polepieces, historically
the SEM industry has seen the large chamber sizes and conical polepieces
you decribe for at least last twenty years. When I entered this
industry in 1973, I remember the JEOL JSM-2 with a semi-conical lens.
The lens was not a true conical lens as it did have a "flat tip" of
about 4 cm.dia. but it did allow one to tilt samples at a higher angle.
I was surprised to see JEOL had a JSM-1 (circa 1969) that had the same
column as the JSM-2.

ISI had a model DS-130 that could be ordered with different final
lenses: the standard semi-conical lens, wide bore (WB) for 0 working
distances, or conical lens. The ISI conical lens had multiple angles on
their design. It started with a 30 degree cone, the mid-section was 45
degree, and ended with a 70 degree cone. For it's day the DS-130 had
pretty good optics. The electronics were junk in my opinion. The DS-130
had some extremly large chambers. One was known by the ISI engineers as
a "turkey baster" chamber. It measured about 24 in. x 36 in. x 18 in
high. Hitachi has had the S-806 and S-808 for semiconductor work. An
full 8-inch wafer could be viewed as well as tilted 45 degrees. Hitachi
also offerred an S-806C which is a conical lens. I believe the vintage
for these machines was in the early eighties.

The JEOL JSM-U3 (circa 1970) had a heated final aperture strip that
allowed the user to clean the aperture without removing it. This
aperture design was replaced in the JEOL 35U and JEOL 35C (circa 1974?).
The JEOL 35 series had a continuously heated final aperture. A smaller
current (about 2 amps) was run through the aperture therby cleaning the
aperture while using it.

I recently (last year) had the opportunity to attend a friend's/customer
retirement party. Attending were many older & retired SEM engineers.
They were reminiscing about the SEM industry and how the machine had
evolved from their days at Westinghouse (yes Westinghouse) in the
1960's. The same questions and problems that we have today they had in
the 1960's. Chamber sizes were initially large to accomodate their
sample and stage sizes but vacuum was poor (10-4 torr). Chamber sizes
were reduced to improve vacuum and lens designs were changed to
accomodate tilt angles at the expense of resolution. And everyone could
only dream of a stable FE gun. SEM nerds.

The upshot of all of this is that all of these issues has been
historically addressed. Large & small chambers, different lenses, etc.
will be with us as long as the application permits. When a manufacturer
claims to have a "breakthough", it only remind me of what Steve Jobs
(Apple Computer) said about Windows 95: " Windows 95: MacIntosh 1986." I
think the next real breakthrough will be the lenses made from
super-conductive wire or, better yet, the electrostatic columns being
developed.

It is my understanding that the electrostatic columns are Shotky FE
sources with backscatter dectectors fully integrated onto the "final
lens". Future features include an integrated EDS detector. The columns
are small & cheap enough to be "thrown away".

I really don't have a disdain for any given Company. I would only like
to comment that every Company has a different "flavor" depending upon
whom is running it. Most are a pleasure to work with, while others leave
a "bad taste in your mouth".

Please don't take these comments personally as I respect your opinion &
have throughly enjoyed the subjects you have brought up on this
listserve & look forward to future your comments.

Regards,

Earl Weltmer



At 01:54 PM 3/16/00 , you wrote:

} Dear Listservers,
}
} I recently responded to a thread about Amray FE maintenance contracts.
} My response contained two errors: one numerical, one in perception.
} I wish to correct them at this time.

[snipped for brevity sake]

Thanks Earl for the update and correction. This is good info. And
I see some room for additional discussion and inquiry to wrap up this
topic.

The prospects for third party maintenance contracts are rather
variable--depending mostly, I think, on what is being maintained
under contract. This would be based on the availability of schematics,
software, special parts, etc., etc. If some FESEM makers do not
"guard" their FE guns relative to others, then of course, that will
weigh
heavily on third party options. For the record, here are some figures
for Amray maintenance contracts. An 1830 LaB6 system runs
$13,500 annually. This excludes "consumables" such as apertures,
LaB6 cathode, pump oil, etc. But it does include on-site service,
troubleshooting, travel, lodging, meals, rental car, things that
break down, like Balzers 240 turbo pump or Edwards E2M8 mechanical
pump, or the invariable broken wire. Actually, I have found the
1830 to be very reliable and a good performer.

The 1910FE runs $15,000 annually. Same terms as the 1830 but
includes the computer control system. What I don't know is whether
Amray will sell the FE gun assemblies to third party maintainers. I do
know that for Amray users who are not under an Amray contract, the
305FE gun costs about $14,000 total to replace as a per-diem item.
Again, I have found that this SEM also is very reliable. But what
about performance?

Performance wise, I suppose we could have an "image shoot off"
contest based on a standard specimen and different SEM
instruments of different models and from different makers. But there
are some basic differences among SEMs that would otherwise
seem to be the same--but are not. Notable among these differences,
for example, is the design and construction of the final lens pole
piece. This is
due, I think, to two or three driving forces. One is the need to image
physically
large specimens at various WDs. This means that one needs a large
chamber
with a stage that offers accommodation of large samples and wide WD
range. The second force is the requirement to image whole semiconductor

wafers ranging in diameters from 4" to 8" and to have a wide travel
distance across the wafer and to operate well at low KV (photo resist
examination,
for example). The third force is the impact on the SEM when needing to
do X-ray analysis.

The flat lens design is quite useable for large and small specimens when

high tilt angles do not increase the WD to an unsatisfactory figure. In
the
case of IC wafers, the conical lens is required. These are available as
45
or 60 degree designs. Since most semiconductor qualification imaging
is done at either zero or 45 degrees, the 60 degree lens is probably
optimal. What Amray has done in regards to conical lenses is an
evolutionary path. The 1830 has a conical lens to accommodate high
tilt angles when working with wafers. It also can be configured with a
large chamber. However, the design of the lens is such that high
resolution is obtained at 10KV-15KV and above. It is not a high
resolution SEM at low KV. Alternatively, with a 25KV or 30KV power
supply, it has ample voltage to support the beam currents needed
for x-ray work. I have no personal information on what the other
makers have done and currently offer in regards to IC wafer inspection
and analysis.

The Amray 3600LEAP was a major design departure. This system
also has a 60 degree conical lens, a huge chamber but a specially
designed lens and other features key to semiconductor work. The
LEAP lens was designed for low KV imaging. At 2-3KV, the LEAP
is virtually identical in resolution to the other lens types at 15KV
and at the same WD. This is a key feature and requirement for
imaging fine pitch and small feature size wafers and photoresist.
The other important feature of the 3600LEAP SEM is the 5-place
heated final aperture holder. Since the 3600 runs with 35u and
70u apertures (or less) as routine, these Pt apertures are
continuously heated to keep the effects of contamination
(photo resist again) at a minimum. Not only does this keep the
apertures clean, but it also prevents accumulation of PR in the
scan coil liner. This FESEM is a very capable instrument from
my experience with it. It can easily image 0.15u wide gate poly
at high resolution and 2.5KV at WD=6mm.

If one considers where I have wound up with this discussion,
it is cause for pause to ask a fundamental question. That is, "What
is a research SEM?" Different applications must surely lead
one to select one type of instrument over another. Does it also
direct a path to one manufacturer over another? Since I have
not seen the myriad number of SEM models from all of the vendors,
I cannot answer this question. But while there might be some
disdain for one maker versus others, it seems tough for me to
make any sort of quantitative decision when there are quite a
few variables involved--solely based on application, much less
vendors and maintenance options.

What do you think about this, based on your experience?

gary g.



From daemon Sun Mar 19 16:09:40 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:10:02 -0500
Subject: Re: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Albek,

If you are looking for the traces of the tools which were used to make
these surfaces, sounds like a job for an interferometer. There are
several small ones which can be used for low to medium power (10x-50x
objective mag) work. Hach used to carry a small Michelson; also, check
with Nikon for both Michelson and Tolansky varieties. While the Tolansky
is a contact method system, it is non-destructive and, since it is a
multiple beam system, gives very high precision results. I would recommend
photographing the interferograms then correlating with the "tooth marks"
left by various tools. The old Polyvar also had an interferometer module.

For a more upscale, automated version, I'd suggest that you find a lab with
either a Zygo New View or a Wyko RST. These devices are Scanning White
Light Interferometers (SWLIs); both are automated, 3D imaging systems which
might provide interesting insight but may also be overkill for your type of
project. Zygo is just south of us, in central Connecticut, while Wyko is
in Tucson. I believe that both have contract labs. (I have done some work
in the Zygo facility)

Please contact me if you are interested in pursuing this approach.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}

At 07:29 PM 12/12/99 +0100, S¿ren Albek wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 19 16:09:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:19:28 -0500
Subject: Re: Quantifying the sharpness of glass knives

Contents Retrieved from Microscopy Listserver Archives
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Dear Rosemary,

A client of ours makes a cut in a polymer block (I can check on the exact
material, if you need) then looks at two parameters:
1) the angle made by the sides
2) the radius of curvature of the bottom

He uses a conventional image analysis system for both, using just the
simple angle measurement function for the first and the diameter of a
circle of best fit for the second. He had been using a stereo microscope
but we took a look at the cut under a 10x objective/compound microscope on
a recent visit and found that there was a lot to be learned about the
quality of the cut from the shards and shredding left on the surface (I
can't remember whether it was the upper or lower, so take a look at both).

Hope this is helpful.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}

At 07:04 PM 3/13/00 -0400, Rosemary Walsh wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 19 16:09:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:25:03 -0500
Subject: Re: Confocal Microscopy in North West Ct

Contents Retrieved from Microscopy Listserver Archives
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Ric,

I think you must be referring to the famous Bill Miller, who was my
"partner in crime" at Sarastro. You can reach him at Bill Miller
{microbill-at-mohawk.net} .

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



At 11:12 AM 3/14/00 -0500, Ric Felten wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 19 16:09:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:58:43 -0500
Subject: Re: Raman microscopy? was Re: water tracer dye?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Raman is going to become as available as regular confocal. As a matter of
fact, it is on the same price scale but can do both confocal and chemical
imaging. It is an analytical technique which works well alongside
fluorescence, but you need to collect the Raman signal separately. I just
came back from PITTCON (THE major analytical chemical meeting) and some
companies are collecting the signal in the near UV, others in the near IR.

A good starting point is Jack Koenig's book "Spectroscopy of Polymers" (Am.
Chem. Soc.. Washington DC, 1992). I just saw him at PITTCON and he said
that the new edition is either just coming out or about to come out.

The move to Raman is part of the merging of microscopy and spectroscopy.
There are now several interesting true hybrid instruments which permit
chemical as well as microscopy imaging. One is the Continuum from
Spectra-Tech (Shelton, CT); the other is a series built on the DuraScope
from SensIR (Danbury, CT). I had a chance to teach a microscopy class to
the IR specialists from SpectraTech in January, so really had a chance to
have my hands on the system. In addition to running full FT-IR chemical
spectra, I was able to do low power darkfield. They also have regular
phase and DIC objectives available (the Phase was a bit tricky to
implement) and have just introduced a fluorescence module.

I will be doing a review for American Lab (Watch for Am Lab: "Focus on
Microscopy") and will post pertinent excerpts for you within the next few
weeks.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}




At 03:00 AM 3/2/00 +0000, Jose Feijo wrote:
} Since the subject was raised, could anyone point me out a good paper or
internet source to understand Raman microscopy, and how to make it work? On
the side, from the gurus, how much should we expect from it in the future?
Is it like, it's going to substitute something already available, or
otherwise it will complement specific aspects of visualisation of some
special biological structure? What does it involve, special lasers, special
optics, special computers?
}
} Thanks in advance
} Jose
}
}



From daemon Sun Mar 19 16:39:44 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 17:25:59 -0500
Subject: Re: Raman microscopy? was Re: water tracer dye?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A propos of our earlier discussion, this just came across the wire from the
Society for Appl. Spectroscopy:

TOUR SPEAKER (Society for Applied Spectroscopy) - 4/12/2000 Meeting in
Mountain View

"RAMAN SPECTROSCOPY AS A PROBE OF STRUCTURE/ORIENTATION IN POLYMERIC
FIBERS"

by DR. BRUCE CHASE (DuPont Central Research, Wilmington DE)

at Michaels at Shoreline, Mountain View (Directions to follow)

Register 6:45 PM
Dinner 7:30 PM
Talk 8:30 PM

Cost: $30 for dinner ($10 students and teachers), free for talk.

Dinner Choices: (Indicate Choice when RSVP'ing)

Breast of Chicken, Piccata
Chilean Sea Bass, Ginger Shallot Sauce
Grilled Vegetable Brochette with Wild Rice

Reserve by April 10 to Steve Rabin, 650-564-5315 or steve.rabin-at-alza.com




Also, see our web site at www.sas-ncss.org

ABSTRACT
Polymeric macroscopic properties such as tenacity, modulus, elasticity,
etc. are determined by molecular level effects such as conformational state
populations, orientation, and intermolecular interactions. Significant
changes occur at the molecular level as a molten or solution phase polymer
is spun into fiber form. The polymer goes from an unoriented, amorphous
state to an oriented, semi-crystalline material via the deformations
imposed by spinning and drawing. Raman spectroscopy can potentially
provide information on both structure and orientation. Changes in band
intensities can be related to conformational populations and to formation
of crystalline regions. Changes in relative intensities as a function of
incident and scattered polarization yields information on chain
orientation. The use of polarized Raman scattering done in both 90 and 180
degree scattering geometries has been used to determine the second and
fourth order orientation functions for both polyethylene and PET fibers.
Raman measurements have been made in both the laboratory frame and on-line
for 66 nylon and PET fibers. For both PET and 66 nylon, crystallinity
levels and a qualitative measure of orientation can be obtained.





From daemon Mon Mar 20 07:46:07 2000



From: IAN HALLETT :      ihallett-at-hort.cri.nz
Date: Mon, 20 Mar 2000 16:45:33 +1300
Subject: Cryo-SEM, mounting frozen powder

Contents Retrieved from Microscopy Listserver Archives
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Dear All

I've been asked whether it is possible to look at a frozen powder
using Cryo-SEM. The powder will be frozen and stored at -80 C and
must be mounted whilst frozen (it may be possible to raise the
temperature to -20 C) any ideas of an adhesive I could use at these
low temperatures??

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hort.cri.nz



From daemon Mon Mar 20 07:46:14 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 20 Mar 2000 08:56:24 +0200
Subject: SEM maintenance & historical info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Earl

} As far as your analysis of the SEM chambers & polepieces, historically
} the SEM industry has seen the large chamber sizes and conical polepieces
} you decribe for at least last twenty years.

For those, like me, who were interested in your recollections about
SEM chamber and lens design, and who may be considering
attending ICEM-15 in South Africa in 2002, make a note that there
will be an exhibition at the congress retracing the history of
electron microscopy in South Africa. This will include a variety of
old EM equipment, including some of the SEM's to which you
referred.

Regards

Rob



Robin H Cross
Chairman : 15th International Congress on Electron Microscopy (ICEM-15)
tel: +27 46 603 8168/9 - fax: +27 46 622 4377
email: r.cross-at-ru.ac.za
http://www.ru.ac.za/emu/icem-15.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Mar 20 07:46:20 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 20 Mar 2000 15:06:28 +0200
Subject: Re: Cryo-SEM, mounting frozen powder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian,
I've used Duro-Takš 80-1061 to hold particles during analysis at liquid
nitrogen temperatures . For more information see:
http://www.fbi.gov/programs/lab/fsc/backissu/july1999/ward.htm

Dennis
________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net


----- Original Message -----
} From: "IAN HALLETT" {ihallett-at-hort.cri.nz}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, March 19, 2000 7:45 PM


Hello Ian

} I've been asked whether it is possible to look at a frozen powder
} using Cryo-SEM.

That shouldn't pose any problems. Choice of adhesive would
depend on the characteristics of the powder.

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Mar 20 07:46:27 2000



From: Carstensen, Jesper Vejl¿
Date: Mon, 20 Mar 2000 13:35:51 +0100
Subject: THANKS: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I would like to thank those who took the time to respond to my e-mail. I
received a lot of valuable information and advice on different techniques to
be used (and NOT to be used) when studying carbides in tool steels. I
appreciate your help.

Kind regards, Jesper

----------------------------------------------------
Jesper Vejloe Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
----------------------------------------------------






From daemon Mon Mar 20 07:57:28 2000



From: Stefan.Geimer :      stefan.geimer-at-uni-koeln.de
Date: Mon, 20 Mar 2000 14:59:40 +0000
Subject: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are planning to buy a new diamond knive for sectioning biological
samples. My question is if anybody has experience with using 35 degree
angle diamond knives and if they have any disantvantages compared to 45
degree angle diamond knives. We are mainly sectioning unicellular algae
embedded in LR-gold and hope to reduce compression problems by using a
35 degree angle knive. Our problem is that, especially algae with thick
cell walls, tend to fall out of the sections. This problem seems to be
caused by compressions, because using a brand-new 45 degree angle
diamond knive I had much less algae falling out of the sections. Because
we can effort only one new knive we have to use it as an all-round
knive. Is a 35 degree knive suitable to replace a 45 degree knive or is
it much more fragile and/or less durable?


Thanks in advance for your time and help.

Sincerely,

Stefan Geimer



***********************
Stefan Geimer
University of Cologne
Botanical Institute
Gyrhofstr. 15
D-50931 Cologne
Germany
phone: +49-(0)221-470-3795
fax: +49-(0)221-470-5181
************************




From daemon Mon Mar 20 13:01:21 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Mon, 20 Mar 2000 10:50:45 -0500
Subject: Liquidation of assets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Apparently there is more equipment lying around that needs a good home. If
anyone is interested, please respond directly to Mark Chambers via
mark-at-semiconductor.com or at (613) 599-6500 ext 4269.

Marisa

} -----Original Message-----
} From: Mark Chambers
} Sent: Friday, March 17, 2000 4:18 PM
} To: Marisa Ahmad
} Subject: RE: Liquidation of assets
}
} Hi Marisa,
}
} Is the listserver you used for the PGT EDS announcement a suitable venue
} for the plasma asher and the Mosaid tester?
}
} The barrel etcher is a Plasmaline model 411 and whoever wants it will need
} to get a vacuum pump for it. The etcher was working but it was not an
} efficient way to decap packages.
}
} The Mosaid tester is a model MS2300 and that's basically all I know about
} it.
}
} Thanks
}
Mark



From daemon Mon Mar 20 13:01:22 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Mon, 20 Mar 2000 09:08:21 -0700
Subject: Re: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You can buy them from Electron Microscopy Sciences. 1-800-523-5874


Soumitra




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Mon Mar 20 13:01:23 2000



From: Bob Phillips :      microservis-at-dial.pipex.com
Date: Mon, 20 Mar 2000 16:34:38 -0000
Subject: JEOL 100CX looking for new home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everybody,

I have been asked to find a new home for a fully working JEOL 100CX tem
which is currently in use in the south of England. It has the ASID stem
attachment, and it could also be available with a Link AN10 analysis system.

Probably the only cost will be the dismantling & removal charges. If anyone
is interested, please reply to me directly. My only commercial interest is
that I might be involved with the dismantling etc.

Bob Phillips
MicroServiS
bob.phillips-at-microservis.co.uk




From daemon Mon Mar 20 13:01:25 2000



From: Dr G. R. Coulton [bs_mp] :      g.coulton-at-ic.ac.uk
Date: Mon, 20 Mar 2000 17:24:44 +0000
Subject: Microscopy/Cell Biology & Imaging at ICHC 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleague,

ICHC 2000

Please check out the web-site for ICHC 2000 (11th International Congress
for Histochemistry and Cytochemistry) 3-8th Sept. York, UK.

We have put together a fabulous speaker list covering a range of the most
important areas of development and application of cutting edge techniques
in Cell Biology and Imaging today. Lead speakers include Roger Tsien,
Stefan Hell, Alan Fine, Alan Bode, Hans Tanke, Jennifer
Lippincott-Schwartz, Angus Lamond, Jim Smith, Richard Haugland, Paul
Monaghan, Mike Ormerod, Simon Gilroy Nick White etc. etc. etc.

www.med.ic.ac.uk/external/ichc_2000

ABSTRACT DEADLINE 15TH MAY 2000.

Best wishes

Gary Coulton
Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

tel 0044 (0)171 594 3190
fax 0044 (0)171 594 3022

PLEASE NOTE AS FROM APRIL 1ST 2000 THE FOLLOWING NUMBERS WILL REPLACE THOSE
ABOVE AND SHOULD BE USED.

TEL. ++44 020 7594 3190
FAX. ++44 020 7594 3022
e-mail g.coulton-at-ic.ac.uk


-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Understanding Biocomplexity: The Post-Genome Challenge"

September 3-8, 2000, York, United Kingdom

ICHC 2000 will comprise 27 symposia addressing the latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

On-line registration now open!!!!!!!!!!

For further details of the meeting and how to pre-register please visit our
web-site at http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you there.



From daemon Mon Mar 20 13:01:26 2000



From: Doug Yates :      dmyates-at-lrsm.upenn.edu
Date: Mon, 20 Mar 2000 12:52:40 -0500
Subject: Technical Staff Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Technical Staff Position:

The Department of Materials Science and Engineering at the University of
Pennsylvania is seeking an engineer or scientist for a technical staff
position in its advanced characterization facility. This facility contains
a number of state-of-the-art microscopes and scattering facilities as well
as ancillary detectors, specimen preparation equipment, and data processing
and computational hardware and software. The facility serves Penn research
programs, as well as academic, industry and government users from the
Delaware Valley region and beyond. The University of Pennsylvania is
located in Philadelphia, one of the nation's most vibrant cities. The
university, a member of the Ivy League, was founded by Ben Franklin and is
the fourth oldest and first secular university in the US. The Laboratory
for Research on the Structure of Matter was constructed to house one of the
original three Materials Research Laboratories (MRLs) in the US. The
university has a continuing tradition of leading materials research and has
housed the MRL (now MRSEC) continuously for 40 years.

Under the direction of the facility manager, the successful candidate will
be responsible for conducting experiments with users on facility equipment,
training new users and maintenance of facility equipment not covered under
service contract. The successful candidate must have the communication
skills and self-confidence to interact with technical users with a wide
range of expertise and background. Job performance will be assessed based
on the success in experimental interactions with users, the operational
state of equipment for which the staff member is responsible, progress in
the acquisition of new skills, and facility appearance.

A Bachelors degree in physical science or engineering is required, although
an advanced degree is preferred. The successful candidate must have
experience with electronics, ultra-high vacuum technology and computer
interfacing of equipment. Experience in the operation and use of electron
microscopes and ion beam lines and associated end-stations is
desirable. Experience in the use of Auger electron spectroscopy or scanned
probe imaging is also desirable.

The text of the official job listing from the University of Pennsylvania
web site follows. Please refer to the Penn Human Resources web site for the
official hiring policy of the university at www.hr.upenn.edu. For
information about the specific position, please contact the facility
manager, Dr. Douglas Yates, at dmyates-at-lrsm.upenn.edu.


Text of web site listing:

Reference Number: 00034808DL
Job Title: RESEARCH COORDINATOR SR
School/Center: ENGINEERING & APPLIED SCIENCE
Department: MATERIALS CHARACTERIZATION
Date Posted: 3/9/00

Salary Grade: 026
Employee Type: Exempt, Monthly Paid
Position Length: Ongoing

Duties: Train or coordinate training of facility users; assist users
performing experiments; compose monthly MCF users billing summary; organize
purchasing of consumable laboratory materials; maintain or coordinate
maintenance of equipment; maintain working environment & appearance of
laboratory.

Qualifications: BA/BS in Physical Science or Engineering required, advanced
degree preferred; 3 to 5 years experience with electronics, ultra-high
vacuum technology & computer interfacing of equipment & in operation & use
of electron microscopes, ion beam lines and/or scanned probe imaging.


*******************************************************
Douglas M. Yates, Ph.D.
Manager, Materials Characterization Facility

(215) 573-6123
dmyates-at-lrsm.upenn.edu

University of Pennsylvania
Department of Materials Science & Engineering
3231 Walnut Street
Philadelphia, PA 19104-6272
*******************************************************



From daemon Mon Mar 20 13:11:50 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 20 Mar 2000 12:00:52 -0700 (MST)
Subject: Re: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

One of your best sources of information is going to be Diatome USA. They
have an actual lab set up to test cutting problems of a large variety.
They also have access to the information of the company who makes these
knives.

Please consider that your problem may not be with the knife or the angle
at which the knife is sharpened. Most "breaking out" problems are related
to the production of the block, especially 1) the infiltration 2) the
match between the specimen and the formulation used. Contact Diatome, at
EMS, and ask to speak with Stacy Kirsch, who is an expert with these
problems. (I have no business interest in Diatome - I have just received
the most excellent advice from them over the years)

Hildy Crowley
Sr. Electron Microscopist
University of Denver
Denver, CO

P.S. Should processing be the basic problem, please contact me. I may be
able to help you.



From daemon Mon Mar 20 13:11:50 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Mon, 20 Mar 2000 11:38:35 -0700
Subject: FW: Need help on PEELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sir,

My specialists tell me, that Boron shows a plasma loss at 22.7 eV and a
K-edge at 188 eV. They also tell me, that it is hard to see B in samples
as it usually disappears rapidly. To see B, they suggest focusing on a
different sample area and then only expose the area in question for the
PEELS acquisition.

In case you are using our software on a LEO microscope for the
acquisition, you should select the MDF option (Micro Dose Focusing).

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Exchange Administrator
Sent: Saturday, March 11, 2000 12:35 PM
To: Michael Bode



Need help on PEELS
}
} To all,
}
} I am working on grain boundary chemistry measurement
} with Digipeels. Try to find out the segregation
} behavior of boron, which is about 0.5at% averagely.
} However, I cannot find any edge for boron in the
} spectrum. As I don't know what's the optimum setting
} for PEELS to check the grain boundary segregation,
if
} you have any idea on this, please let me know.
}
} Thanks,
} Lung
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Mon Mar 20 17:55:28 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Mon, 20 Mar 2000 13:50:34 -0800
Subject: JEOL 100SX going to salvage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We bought a JEOL 100SX for about $130,000 in 1987. The scope was for
student use in an undergraduate course. It was used for 4 to 8 weeks per
year for 7 years. It was used very little. About 1994, the water chiller
failed and cooked the objective mini-lens and some other electronics. It
was one year off the service contract. JEOL could not guarantee that they
could fix it for less than $11,000 so it was moth balled. It was
functional at 8000X and higher. We have to get rid of this in a real
hurry. It someone wants some or all of this machine please contact me
ASAP. Within about a week it will be in the dump. I recall that at the
time it failed there was a user at Stanford (I think) who had the same
model. If anyone knows who that was, please have him contact me.




Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Mon Mar 20 17:55:29 2000



From: Everett Ramer :      Everett.Ramer-at-netl.doe.gov
Date: Mon, 20 Mar 2000 17:22:28 -0500
Subject: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




It seems that nowadays every SEM vendor is offering variable pressure models, which are conventional SEMs with a plumbing modification that allows the sample to be at pressures of up to about 4 torr (500 Pa) while the electron column operates at the conventional high vacuum. I am very interested in buying a variable pressure SEM with an EDS, but was recently warned that EDS has very poor spatial resolution in the variable pressure mode because of the large beam spread due to electrons scattering off the gas molecules in the sample chamber. Is this really a significant issue? Do any of you have experience using EDS with variable pressure SEMs?
Thanks,



From daemon Mon Mar 20 17:55:34 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 20 Mar 2000 17:09:07 -0600
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In short, it is an issue, but not intractable. For careful quantitative
work we reduce the vacuum as much as the sample permits. We can usually get
by with about 40 Pa of helium atmosphere on concrete samples. Even at 40 Pa
we can pick up percent levels of elements away from the beam. For example,
a Co chip in a standard mount can easily show 1% Fe from the stainless
steel carrier. For qualitative work, that is not much of a problem, but if
you are looking for trends in that element (Fe in Co), it would be
impossible in low vacuum mode.

BTW, Helium greatly reduces the scattering compared to air at the same
pressure.

I would encourage you to go ahead and get the VP SEM, but work through a
few exercises with it to get a feel for its limits.

At 05:22 PM 3/20/2000 -0500, you wrote:
} It seems that nowadays every SEM vendor is offering variable pressure
} models, which are conventional SEMs with a plumbing modification that
} allows the sample to be at pressures of up to about 4 torr (500 Pa) while
} the electron column operates at the conventional high vacuum. I am very
} interested in buying a variable pressure SEM with an EDS, but was recently
} warned that EDS has very poor spatial resolution in the variable pressure
} mode because of the large beam spread due to electrons scattering off the
} gas molecules in the sample chamber. Is this really a significant issue?
} Do any of you have experience using EDS with variable pressure SEMs?
} Thanks,

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Mar 20 18:28:24 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 20 Mar 2000 16:01:36 -0800
Subject: Re: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is a good point to contact DIATOME. It reminds to me, also, that they
(DIATOME) may cut your samples on site and sent back the grids (if I
understand them correctly) for free. So, you may try 45 and 35o knife and
compare the results before you make final decision. As for 35o. 45o is
more universal and durable. If you rich enough only for one diamond knife,
it should be 45o, I believe. 35o supposed to be a "second knife" for
"special occasions". I have no financial interest in DIATOME, but happy
user of DIATOME knife

Sergey

} Date: Mon, 20 Mar 2000 12:00:52 -0700 (MST)
} From: HILDEGARD CROWLEY {hcrowley-at-du.edu}
} Subject: Re: 35 Degree Angle Diamond Knive
} X-Sender: hcrowley-at-odin.cair.du.edu
} To: "Stefan.Geimer" {stefan.geimer-at-uni-koeln.de}
} Cc: Microscopy-at-sparc5.microscopy.com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Mon Mar 20 18:58:31 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Mon, 20 Mar 2000 19:49:35 -0500
Subject: RE: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Diatome, for one, can supply you with clear ev idence for significantly
reduced compression with a 35 degree knife over a 45 degree knife. No
surprise there, since compression should be reduced with knife angle in
materials which compress significantly. We are a 'materials science' lab,
where we section all manner of metals and alloys, composites, powders,
fibres, coatings, thin films, etc, etc. For us reduced compression is not a
big deal, but durability obviously is. I can report that, for the last 5
years or so since their acquisition, our two Diatome 35 degree knives are
used about 90% of the time, with no catastrophic failures or large increase
in sharpening frequency.

I agree with Hildegard Crowley that breaking out is far more likely to be
due to embedding/bonding problems. another possibility is simply a dull
edge. Your increased success with a fresh (presumably demo) knife could
indicate the latter. If the edge is quite rounded from use, the effective
angle at the point of sectioning will shoot up incredibly, which will then
increase the compressive forces that expose any embedding/bonding
deficiencies.

That said, if you can only afford one knife, look in the mirror and ask
yourself how careful are the people who might use the knife. A simple
consideration of the forces on a knife edge during sectioning will tell you
that a lesser angle should be slightly more susceptible to side forces.
However, just the thought of a reduced angle might make an inexperienced
operator sufficiently nervous to increase that risk greatly!

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


} ----------
} From: Stefan.Geimer
} Sent: Monday, March 20, 2000 9:59 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: 35 Degree Angle Diamond Knive
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are planning to buy a new diamond knive for sectioning biological
} samples. My question is if anybody has experience with using 35 degree
} angle diamond knives and if they have any disantvantages compared to 45
} degree angle diamond knives. We are mainly sectioning unicellular algae
} embedded in LR-gold and hope to reduce compression problems by using a
} 35 degree angle knive. Our problem is that, especially algae with thick
} cell walls, tend to fall out of the sections. This problem seems to be
} caused by compressions, because using a brand-new 45 degree angle
} diamond knive I had much less algae falling out of the sections. Because
} we can effort only one new knive we have to use it as an all-round
} knive. Is a 35 degree knive suitable to replace a 45 degree knive or is
} it much more fragile and/or less durable?
}
}
} Thanks in advance for your time and help.
}
} Sincerely,
}
} Stefan Geimer
}
}
}
} ***********************
} Stefan Geimer
} University of Cologne
} Botanical Institute
} Gyrhofstr. 15
} D-50931 Cologne
} Germany
} phone: +49-(0)221-470-3795
} fax: +49-(0)221-470-5181
} ************************
}
}
}
}



From daemon Mon Mar 20 19:28:22 2000



From: maxwell-at-lec.med.utoronto.ca () (by way of Nestor J. Zaluzec)
Date: Mon, 20 Mar 2000 19:20:58 -0600
Subject: bacteriophage question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

Can anyone help Karen Maxwell?, reply to her as well as the list
as she is not a subscriber.

Nestor
Your Friendly Neighborhood SysOp



Below is the result of your feedback form. It was submitted by
(maxwell-at-lec.med.utoronto.ca) on Wednesday, March 15, 2000 at 17:03:53
---------------------------------------------------------------------------

Email: maxwell-at-lec.med.utoronto.ca
Name: Karen Maxwell

School: University of Toronto

State: Ontario

Question: I have been looking at a paper from 1984 (Kochan et al) where
they used EM to study the preconnector from bacteriophage lambda. They
discovered that the axial hole in the ring shaped structure was 2.2-2.5 nm
in diameter. They used negative staining with ammonium molybdate, uranyl
acetate, and uranyl formate. In more recent studies (Valpuesta et al,
1999), the connector of phi29 was examined using a three-dimensional
cryo-reconstruction, and the axial hole was found to be 3.3 nm. These are
two different phage, but my question is, could the old techniques used in
the case of the bacteriophage lambda preconnector (negative staining) have
under-estimated the diameter of the axial hole? And if so, do you have an
idea of the percent error that may be inherent in the technique? Is the
cryo-reconstuction likely to give a value that is more accurate? Could you
recommend any references that would address these questions?

Thanks in advance for your help.

Karen Maxwell

---------------------------------------------------------------------------




From daemon Mon Mar 20 21:28:19 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Mar 2000 19:16:10 -0600
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:22 PM 3/20/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have not had an opportunity to try these systems but from what I can
glean, they are not totally similar to conventional SEMs. In particular,
the VP SEMs do not use the E-T detector, but rather a gas discharge
detector. There are some good examples of these images around but
I do wonder what the actual difference is. Perhaps owners can tell us.
Or warn us???

gary g.



From daemon Tue Mar 21 07:32:46 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Mar 2000 19:16:10 -0600
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:22 PM 3/20/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have not had an opportunity to try these systems but from what I can
glean, they are not totally similar to conventional SEMs. In particular,
the VP SEMs do not use the E-T detector, but rather a gas discharge
detector. There are some good examples of these images around but
I do wonder what the actual difference is. Perhaps owners can tell us.
Or warn us???

gary g.




From daemon Tue Mar 21 07:32:48 2000



From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Tue, 21 Mar 2000 10:03:54 +0100
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Everett Ramer wrote:
} It seems that nowadays every SEM vendor is offering variable pressure
} models, which are conventional SEMs with a plumbing
} modification that allows the sample to be at pressures of up to
} about 4 torr (500 Pa) while the electron column operates at the
} conventional high vacuum. I am very interested in buying a
} variable pressure SEM with an EDS, but was recently warned that
} EDS has very poor spatial resolution in the variable pressure
} mode because of the large beam spread due to electrons
} scattering off the gas molecules in the sample chamber. Is this
} really a significant issue? Do any of you have experience using
} EDS with variable pressure SEMs?
} Thanks,

The fraction of primary electrons that are scattered by the gas in
the specimen chamber is approximately given by:

1-I/Io = 1 - exp(-psL/kT)

where p is the pressure
s the total scattering cross section for scattering of x keV
electrons on the gas used
L the distance between the last pressure limiting aperture and
the sample
k the Boltzmann constant
and T the absolute temperature.

You can therefore reduce the contribution of X-rays excited by
scattered primary electrons by reducing p (lowest possible
pressure), s (use gas with low scattering cross section and the
highest possible acceleration voltage balanced against
overvoltage considerations) and L.

As Warren Straszheim wrote this will overcome the beam skirt
problem to a large degree. In the situations where this is not
sufficient, you can use an extrapolation method that has been
developed here at Risoe National Laboratory. In short, you
perform the analysis at various different pressures and use the
above equation to extrapolate to the result you would get under
zero pressure. A more detailed description can be found at the
web-address

{ HYPERLINK http://www.risoe.dk/afm/news1new.htm }http://www.risoe.dk/afm/news1new.htm

Best regards,
Joergen Bilde-Soerensen


*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



From daemon Tue Mar 21 07:32:53 2000



From: GuessWho :      olivier.balmes-at-oorg2.lth.se
Date: Tue, 21 Mar 2000 11:26:23 +0100
Subject: EFTEM/GIF on particles

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I was wondering if anybody was using a GIF filter. We have one mounted
on a 120 kV TEM and are trying to image small particles (5-10 nm) and
doing some energy filtering to image different elements in the particles
(for example gold, silicon, copper, cerium...) . This is not really what
I would call trivial work and we are still working out the set up
(window size and positionning for example) in a rather empiric way. The
main problem is often to get a decent signal in the interesting energy
region and still keep the windows fairly narrow.
I would add that we often have to work at low dose because of the beam
sensitivity of our samples, but I guess our GIF setups can be improved.
Any suggestion ?

Any general comment on the GIF warmly welcome !

Thanks

Olivier



From daemon Tue Mar 21 07:32:55 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 21 Mar 2000 11:29:14 -0000
Subject: Hoechst 33258 counter stain

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Dear Collegues

I am staining virus infected cell DNA (in cell culture) with Hoechst 33258
fluorescent stain. I need to make correlation of the fluorescent spots with
cellular structure.

Do you know of any counter-stain that can be used without destroying the
fluorescent Hoechst staining?

Thanks
Dr. A.P. Alves de Matos
biologist
apmatos-at-ip.pt



From daemon Tue Mar 21 07:32:56 2000



From: Florian Dalla Torre :      Florian.dallatorre-at-psi.ch
Date: Tue, 21 Mar 2000 13:30:29 +0100
Subject: Electrolytical thinning of nanocrystalline Nickel for TEM

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Hi all,

Does anybody now the solution, temperature and voltage for thinning
electrolytically Nickel (nanocrystalline) for TEM samples?

--
Florian Dalla Torre
Paul Scherrer Institut
CH / 5232 Villigen PSI
Switzerland
email: Florian.DallaTorre-at-psi.ch
phone ++41/56/310 35 66
fax ++41/56/310 31 31




From daemon Tue Mar 21 07:50:41 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Tue, 21 Mar 2000 07:37:17 -0600
Subject: RE: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
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Diana:
TAAB Laboratories Equipment, Ltd,
3 Minerva Court House,Calleva Park
Aldermaston, Berks,RG7 8NA, UK

They list single edge razor blades, with and without a bac k in
stainless and carbon steels. They also list double edge razor blades, 0.004
in. thick, in stainless steel.

Best regards,

Sam Purdy
} ----------
} From: Jeff & Wanda Gray
} Sent: March 2000 10:12 PM
} To: Diana Papoulias; microscopy-at-sparc5.microscopy.com
} Subject: RE: accu-edge blades
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Diana,
} These blades are available from Allegiance Scientific (used to be Baxter,
} used to be Scientific Products, used to be....). As far as I know, this is
} the only place that carries this brand.
} I have no financial interest in Allegiance, just passing along a fact.
} Wanda Shotsberger
} (HT ASCP)
}
} -----Original Message-----
} } From: Diana Papoulias [mailto:Diana_Papoulias-at-usgs.gov]
} Sent: Thursday, March 16, 2000 2:53 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: accu-edge blades
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Could someone please tell me where I can purchase accu-edge microtome
} blades?
}
} Thank you.
}
} Diana Papoulias
} USGS
} 4200 New Haven Rd
} Columbia, MO 65201
}
} T:573 876 1902
} F:573 876 1876
} E:Diana_Papoulias-at-usgs.gov
}
}
}




From daemon Tue Mar 21 07:57:26 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Tue, 21 Mar 2000 14:53:31 +0100
Subject: RE: EDS in Variable Pressure SEM

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Dear Everett,

The extrapolation method (aka Variable Pressure Method) described by Dr.
Bilde-Sorenson has been implemented by EDAX in a software feature called
ViP-Quant. With this method the EDS analysis under Bad-Vacuum conditions can
be done with nearly the same accuracy as under High-Vacuum conditions.
Although this method was developed with the ESEM microscope in mind, it of
course can be applied to all Bad-Vacuum scanning electron microscopes.

Please contact your local EDAX representative for more information and a
copy of the new ViP-Quant brochure, or request one through www.edax.com.

With best regards,

Hans Dijkstra

-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
} From: Joergen Bilde-Soerensen 5802 [mailto:j.bilde-at-risoe.dk]
} Sent: Tuesday, March 21, 2000 10:04 AM
} To: Everett Ramer
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: EDS in Variable Pressure SEM
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Everett Ramer wrote:
} } It seems that nowadays every SEM vendor is offering variable pressure
} } models, which are conventional SEMs with a plumbing
} } modification that allows the sample to be at pressures of up to
} } about 4 torr (500 Pa) while the electron column operates at the
} } conventional high vacuum. I am very interested in buying a
} } variable pressure SEM with an EDS, but was recently warned that
} } EDS has very poor spatial resolution in the variable pressure
} } mode because of the large beam spread due to electrons
} } scattering off the gas molecules in the sample chamber. Is this
} } really a significant issue? Do any of you have experience using
} } EDS with variable pressure SEMs?
} } Thanks,
}
} The fraction of primary electrons that are scattered by the gas in
} the specimen chamber is approximately given by:
}
} 1-I/Io = 1 - exp(-psL/kT)
}
} where p is the pressure
} s the total scattering cross section for scattering of x keV
} electrons on the gas used
} L the distance between the last pressure limiting aperture and
} the sample
} k the Boltzmann constant
} and T the absolute temperature.
}
} You can therefore reduce the contribution of X-rays excited by
} scattered primary electrons by reducing p (lowest possible
} pressure), s (use gas with low scattering cross section and the
} highest possible acceleration voltage balanced against
} overvoltage considerations) and L.
}
} As Warren Straszheim wrote this will overcome the beam skirt
} problem to a large degree. In the situations where this is not
} sufficient, you can use an extrapolation method that has been
} developed here at Risoe National Laboratory. In short, you
} perform the analysis at various different pressures and use the
} above equation to extrapolate to the result you would get under
} zero pressure. A more detailed description can be found at the
} web-address
}
} { HYPERLINK http://www.risoe.dk/afm/news1new.htm
}http://www.risoe.dk/afm/news1new.htm

Best regards,
Joergen Bilde-Soerensen


*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm




From daemon Tue Mar 21 07:57:26 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Tue, 21 Mar 2000 07:54:37 +0000
Subject: Opening Leica TCS SP files

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Does anyone have a macro to open up individual channels of Leica TCS
confocal files for NIH/Scion Image?
Thanks!
ED



From daemon Tue Mar 21 18:21:36 2000



From: Centro de Im‡genes y Microscop’a
Date: Tue, 21 Mar 2000 08:02:51 -0600
Subject: fixating and including frozen pieces

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Dear Colleagues,
I want to fix insects at particular moments of muscle activity. I
plan to
freeze the bugs in liquid nitrogen and section their heads, after including
them in hard Durcupan. I have no experience in fixating and including
frozen pieces. Note that hard resin should be used, provided the hardness
of the insect cuticle. Does anybody know a simple method for the
transference from liquid nitrogen to plastic?
Thank your very much in advance,
Claudio
Dr. Claudio R.Lazzari
lazzari-at-bg.fcen.uba.ar

------------------------------------------
Laboratorio de Fisiolog’a de Insectos
Dpto. Cs. Biol—gicas, Univ. Buenos Aires
Ciudad Universitaria, 1428 Buenos Aires
Argentina
Tel.(54 11) 4576 3300, ext. 332, FAX (54 11) 4576 3384




Gabriel Adriano Rosa
Centro de Imagenes y Microscopia
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
Ciudad Universitaria, 4 piso, Pab. II, CP 1428, Buenos Aires, ARGENTINA
TE -(54-11)-4576-3349 e-mail cimic-at-bg.fcen.uba.ar
FAX (54-11)-4576-3384




From daemon Tue Mar 21 18:21:37 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 21 Mar 2000 09:36:07 -0500 (EST)
Subject: Re: DNA stain summary

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I don't think 7-AAD will work - it is used as a live/dead stain, and I
know that it works much better on detergent-permeabilized cells than on
merely PFA-fixed ones.

If you are contemplating microinjection (ugh!), maybe try the fluorescent
dextrans instead of UTPs...they won't mess up your nucleic acids. We use
them as injection markers and they don't seem to harm the cells.

Tamara Howard
CSHL

On Tue, 21 Mar 2000, Donald O'Malley wrote:

} Hi Folks,
}
} Thanks for the flurry of info about DNA/RNA staining. I've
summarized some of this info below in case anyone else has needs related
to ours.
} What I neglected to mention is that we are trying to count nuclei
inside of living mouse embryos. That's why we're searching for a
non-invasive, membrane crossing, nuclear-selective dye.
}
} Summary of recent listserv messages:
}
}
} Possible Dyes for live TISSUE, visible wavelength,
} selective NUCLEAR staining:
}
} 7 aminoactinomycin. But does it cross membranes?
} microinjected Alexa-UTPs



From daemon Tue Mar 21 18:21:38 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 21 Mar 2000 09:50:23 -0500
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Everett,

I just got back from PITTCON. When I was visiting the EDAX booth they
showed me a new program through which they have resolved this problem. I
would suggest at least inquiring.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.


At 05:22 PM 3/20/00 -0500, Everett Ramer wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Mar 21 18:22:02 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Tue, 21 Mar 2000 17:12:39 -0500
Subject: Hoechst 33258

Contents Retrieved from Microscopy Listserver Archives
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Dr. Alves de Matos,
Regarding your question about counter staining for cellular structure--have
you considered scanning your Hoechst image and overlaying a Nomarski
image? If this doesn't yield enough detail there are fluorescent dyes
specifically for organelles such as mitochondria and golgi. Molecular
Probes sells them.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Tue Mar 21 18:22:05 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 21 Mar 2000 17:59:40 -0600
Subject: Microscope Digital Cameras (Firewire?)

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_____________________________________________________

Karl E. Garsha, Doctoral Student
AOP Fellow
Chief Coordinator-Graduate Student Association
Department of Biological Sciences
University of Wisconsin-Milwaukee
P.O. Box 413
Milwaukee, WI 53201
Office: 459 Lapham Hall
Phone: (414) 229-4316
Mobile: (414) 617-4295
E-Mail: keg-at-csd.uwm.edu

----------
} From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
To: maxwell-at-lec.med.utoronto.ca


Firewire Camera Anyone?
Does anyone know of, or have information on current or upcoming digital
cameras for light microscopy that utilize the Firewire or USB interface for
quick and easy importing of light microscope images through a color digital
camera, for example to a Mac G4 (or even the iMac G3)? Does this
technology exist in a microscope camera? (yet)




From daemon Tue Mar 21 18:52:11 2000



From: RCHIOVETTI-at-aol.com
Date: Tue, 21 Mar 2000 19:33:11 EST
Subject: Re: Microscope Digital Cameras (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 03/21/2000 5:15:50 PM US Mountain Standard Time,
HUGGINBJ-at-BP.com writes:

{ { Firewire Camera Anyone?
Does anyone know of, or have information on current or upcoming digital
cameras for light microscopy that utilize the Firewire or USB interface for
quick and easy importing of light microscope images through a color digital
camera, for example to a Mac G4 (or even the iMac G3)? Does this
technology exist in a microscope camera? (yet)
} }

The only one I am aware of is the Optronics MagnaFire, which is a cooled
camera with the IEEE-1394 FireWire interface.

Previous versions of the Optronics cameras used a parallel (printer) port
interface so they had to be used on PC's only. It would be worth inquiring
about Mac compatibility. Sorry I don't have the answer to this part...I only
know the MagnaFire has the FireWire interface.

Visit {http://www.optronics.com} and click on "MagnaFire" for specs.

Cheers,

Bob Chiovetti



From daemon Tue Mar 21 20:02:03 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Tue, 21 Mar 2000 17:48:15 -0800 (PST)
Subject: Film Scanner Recommendations?

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Hi all-

We are in the midst of writing a proposal for a new film scanner and I
am hoping that someone out there can help with a recommendation for a top
of the line film scanner. The major application would be TEM negatives. A
model we are currently looking at is the Nikon LS-4500AF Multi Format Film
Scanner.

If anyone has archived recommendations from previous discussions and could
send them on to me, that would be wonderful.

Thank you,
Valerie Leppert

Dept. of Chem. Eng. and Mat. Sci.
U. of California, Davis

vjleppert-at-ucdavis.edu



From daemon Tue Mar 21 20:18:59 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Tue, 21 Mar 2000 21:07:37 -0500
Subject: Re: USB or Firewire Cameras

Contents Retrieved from Microscopy Listserver Archives
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I do not work for Optronics, but know a Mac version of Drivers is due
out very
shortly for the Magnafire Camera. For more information, check out....

http://www.optronics.com

Also, Nikon has an SLR based 2.74 megapixel Digital Camera suitable for
most
Microscopy (including bright emission Fluorescence with higher ISO)
called the
D1, based on a N90s Body with a F-mount and Firewire connection. Approx.
$5300

http://www.nikonusa.com/products/detaild1.cfm?id=286

Nikon also has a much less expensive digital camera Coolpix 990 coming
in the
next few weeks. This is a 3.34 million pixel, true resolution camera
with live
NTSC out (for simultaneous real-time video) and USB connectivity with
an
Electronic Shutter Release built-in for around $1000. It too, can handle
most
Microscopy techniques, but is limited in fluorescence by its 100 ISO
rating. It
is a C-mount type camera.

http://www.nikonusa.com/products/detaila.cfm?id=282

Regards,

Lawrence Kordon
Nikon, Inc.
nikon-at-jagunet.com

**I have no corporate affiliation with/nor any financial agreement with
Optronics, Inc. I do however have strong ties with Nikon (as you can
see), but
make very little money from either product ;)



"RCHIOVETTI-at-aol.com"-at-sparc5.microscopy.com wrote:

}
------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.

}
} In a message dated 03/21/2000 5:15:50 PM US Mountain Standard Time,
} HUGGINBJ-at-BP.com writes:
}
} { { Firewire Camera Anyone?
} Does anyone know of, or have information on current or upcoming
digital
} cameras for light microscopy that utilize the Firewire or USB
interface for
} quick and easy importing of light microscope images through a color
digital
} camera, for example to a Mac G4 (or even the iMac G3)? Does this
} technology exist in a microscope camera? (yet)
} } }
}
} The only one I am aware of is the Optronics MagnaFire, which is a
cooled
} camera with the IEEE-1394 FireWire interface.
}
} Previous versions of the Optronics cameras used a parallel (printer)
port
} interface so they had to be used on PC's only. It would be worth
inquiring
} about Mac compatibility. Sorry I don't have the answer to this
part...I only
} know the MagnaFire has the FireWire interface.
}
} Visit {http://www.optronics.com} and click on "MagnaFire" for specs.
}
} Cheers,
}



From daemon Wed Mar 22 07:51:43 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 21 Mar 2000 23:09:26 -0800
Subject: FW: neg stain vs cryo EM plus image averaging

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Dear Karl
I disagree with you, that "UA in particular, will positively stain
proteins". Let start from definition: positively stain object is a dark
object on the light background (therefore stain penetrate/interact with
object making it "stained", dark in the terms of EM); "negative staining"
(NS) is opposite. We have light (stain do not interfere with the sample)
object on the dark background of stain (or dark ring of stain around the
object, depends from stain and technique). The beauty of the NS is that
stain (UA in particular) easily penetrates cavities in the object making
visible fine details (for instance, I was able to recognize variable and
constant domains of the Fab of the IgG molecules as well as domains of the
Fc using UA staining). May be this effect you call "positively staining".
But it is not.

Yes, UA may stain RNA and DNA positively. For this reason UA gives us
"mixed stain" for ribosomes (negative for proteins and positive for RNA
components). The disadvantage UA is its low pH.

As for subject of the current discussion. I think Cryo in general will
give us more correct information than others EM techniques. But, we have
to keep in mind, that in Cryo-technique, to make image relatively contrast,
people should go to very high overfocusing (from half to couple microns, I
believe). For this reason, the best Cryo-results I know, yielded only 2-3
nm resolution (and this is a huge problem in Cryo - to get better
resolution): the same or even worse as for "negative staining" (I expect
1.5 nm resolution for UA). We have to count resolution when compare the
data. I lost the original message from which this discussion was started,
but it seems to me, that difference between NS and Cryo was not so dramatic
and may be comparable if we will count the resolution. In general, I don't
believe any EM data if resolution is not shown. Talking about resolution
is complicated because of the difference between "resolution of the
instrument" (0.14 nm for TEM currently), "physical resolution of the
method" (resolution limit for overfocusing, for instance, radiation damage,
drift, noise/signal ratio etc) and "resolution of the sample" (for
biological samples they are not the same: preparation of the sample may
affect sample's intactness/structure). Cryo is the best for sample
preservation, but it is low in contrast. UA may affect sample's structure
(may not) but the resolution limited only by granularity of stain (in
theory it is a couple angstroms).

As for "averaging" of the images, I am a little bit skeptical about that.
It is great deal if your sample is uniform in shape (symmetry is a great
help too). If your biological sample is functionally flexible (IgG for
instance), averaging will "hide" some interesting details even if you will
distribute images in classes.

In my point of view, it is difficult to extract absolute numbers from EM.
Inside one methodology you could easily compare the data, but it becomes
difficult when you want to compare the data obtained by different
techniques. This is reality of EM. I think Scanning Probe Microscopy is
very promising to obtain the direct measurements on the samples under
physiological conditions (EM is so far from "physiological conditions",
unfortunately). I am so sorry, EM!

} Date: Mon, 20 Mar 2000 16:46:25 -0600
} From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
} Subject: FW: neg stain vs cryo EM plus image averaging
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: Microsoft Outlook Express for Macintosh - 4.01 (295)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 22 07:51:43 2000



From: A. P. Alves de Matos :      apmatos-at-ip.pt
Date: Qua, 22 Mar 2000 07:13:44 +0000
Subject: Hoechst counterstain

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Dear collegues

I posted a previous message asking for a counterstain for Hoechst 33258, however I fail to mention that I want to use the
counterstain in brignt field ilumination, switching to fluorescence as needed. Something that reveals celular structure like Giemsa
would be OK. Giemsa however does not alow simultaneous staining with Hoechst as far as I can see.

Thanks
Dr. A.P. Alves de Matos
Biologist




From daemon Wed Mar 22 07:51:45 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 22 Mar 2000 09:10:12 +0100 (MET)
Subject: Re: EFTEM/GIF on particles

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Hello,

Regarding EFTEM on small particles, this should actually work quite well.
That is, elemental mapping works reasonably if you choose the right
objective apperture. You should simulate the spatial resolution to see
what can be attained under your conditions (HT, Cc, Cs etc).
However it will be very difficult to get real concentration information
out of these images. I'm trying EELS with nanoprobe but so far no succes
(everything gets destroyed before I take a spectrum, allignment is very
very difficult)
Still, focussing remains a problem. Comon practice is to focus at 100eV
and then suppose everything is OK for higher losses. I do not understand
the theory of this technique (blurring by finite slitwidth & Cc?) nor thus
it work well. Any comments on this are welcome.

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************

On Tue, 21 Mar 2000, GuessWho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} I was wondering if anybody was using a GIF filter. We have one mounted
} on a 120 kV TEM and are trying to image small particles (5-10 nm) and
} doing some energy filtering to image different elements in the particles
} (for example gold, silicon, copper, cerium...) . This is not really what
} I would call trivial work and we are still working out the set up
} (window size and positionning for example) in a rather empiric way. The
} main problem is often to get a decent signal in the interesting energy
} region and still keep the windows fairly narrow.
} I would add that we often have to work at low dose because of the beam
} sensitivity of our samples, but I guess our GIF setups can be improved.
} Any suggestion ?
}
} Any general comment on the GIF warmly welcome !
}
} Thanks
}
} Olivier
}
}
}



From daemon Wed Mar 22 07:51:54 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Wed, 22 Mar 2000 06:49:33 -0500
Subject: Buying a SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

A few weeks ago, when our server was working correctly (*??!!*), there were
e-mails talking about the purchase of a new SEM. Now we appear to be back
on line I felt that our ideas may be of use to the mailers?

As part of the consultancy side of our business we purchase SEM for our
clients. This means we are buying instruments regularly and are therefore
probably one of the few professional SEM purchasing organisations?

In our team we have staff who have worked for most of the SEM manufacturers
as demonstrators and application specialists, so we have seen the purchase
game from both sides and of course try to use this to our clients
advantage. Unlike the average microscopist, who probably only buys two
instruments in their career, we have been able over the years to formulate
a purchase plan, a purchasing criteria that we apply to each purchasing
project. In our position we are unable to buy from the nicest salesman or
the normal "gut" feeling, we must be absolutely certain that we are
purchasing the best instrument for our clients applications.

Set out below are the headings from a lecture that I give on instrument
purchase. Only the basic ideas are presented ( I guess if you need more I
shall be attacked by Microscopy Today to give just that) but you will get
the theme. It is important to realise that the specimen chamber of a SEM,
the specimen-detector geometry, determines what we will see. For this
reason you will find that one instrument will do a particular task better
than any other in the price range, you need test specimens that will sort
this out for you. If you have a multi instrument laboratory I always feel
that it is a good idea to have instruments from different manufacturers
optimising the laboratory for optimising a wider range of applications.

"Buying a New Microscope"

How Do You Start?

Collect ALL the brochures and prices
Form a view of the new facilities being made available
If you do not understand any features ASK the salesman
Check through all the users desired facilities?
Determine who will use the instrument now?
Would there be others if you purchased particular accessories?

Formulate a Purchase Specification

Price range - we always look one level higher
Set essential instrument specifications
Set desired instrument specifications give them points and produce a
"desirability assessment"

Why use a consultant?

Only he will without prejudice talk to all interested parties
Interdepartmental feuds could spoil the case
A different questioner provides different answers
Their wide knowledge base may develop additional features
They will have experience producing a "desirability assessment" which often
brings surprises.
They will be experienced with ways of dealing with the sales staff, they
will keep them off your back

How to handle the demonstration? DO NOT LET THEM

Show you how wonderful the alignment procedures are - you will not spend
all day aligning the instrument!
Dominate YOUR demonstration
Take you into totally uninteresting areas of the instrument - you should be
buying because of the image quality
Show their favourite gimmick
Use their own favourite specimen
Take you out of the room when about to load one of your specimens
Take you to a two hour lunch with lots of drink

How to handle the demonstration before you set off

Select a maximum of three specimens that you know really well and that are
important to your laboratory
The specimens must be capable of meaningful low and high magnification
imaging
Develop a demonstration criteria for each specimen
Have a tie break specimen available as suggested by the consultant
Provide each company with an exact demonstration programme

What a consultant would do during a demo

Time the demonstrator to produce images at specific magnifications under
very specific conditions
Encourage the demonstrator (this is not a customer v demonstrator
competition) allow them to try their own ideas with each specimen also,
give them information that you have found to be of importance with your
specimens
Stop the demonstrator taking you away from your standard path - you do wish
to compare each instrument under exactly the same conditions there is no
time for other deviations

After the demo

Layout the results
Compare performance instrument to instrument and time taken to obtain the
results
Produce a short list
Compare the short list with the "desirability assessment"
Research local and national service performance of the best two instrument
manufacturers, and the manufacturers reputations

Finally
Decide upon the instrument that you want, the specification and then haggle

Hope this helps?

Steve Chapman
Senior Consultant
Protrain for Training and Consultancy in EM World Wide
Tel & Fax 44+ 1280 814774
e-mail protrain -at-emcourses.com
web site www.emcourses.com



From daemon Wed Mar 22 07:52:03 2000



From: Griffith, Ed :      EGriffith-at-FEICO.COM
Date: Wed, 22 Mar 2000 05:28:05 -0800
Subject: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Huggins:

The ESEM with a cryostage has been used to observe such things as ice cream
and asphalt/solvent mixtures. The ice cream work was published out of the
Cavendish Labs [THiel, Donald]. The other was a lab experiment.

Hope this helps

Ed G



From daemon Wed Mar 22 08:22:05 2000



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Wed, 22 Mar 2000 10:13:39 -0400
Subject: TEM-shadow coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
I have problem obtaining a good replica using a rotary shadow coating
method. Samples are proteins suspended in a Tris, NaCl and CaCl2
buffer. They are absorbed onto a mica and dried in vacuum. I am using
platinum wire (0.1 mm in diameter, 8 cm long) wrapped around tungsten
filament followed by carbon coating. This replica is then
transferred onto the grids. Coating is done in Hitachi HUS 5GB
vacuum evaporator: vacuum 10-6 torr, 20 mA current through the
electrode for 12 sec. The distance from the electrode to the plate
with the samples is 10 cm. The problem is that I get very little
coating (so little that there is no replica) and if I go higher then
20 mA tungsten brakes. I have no problem when I use palladium/gold
wire with same parameters but that gives coarse granulation. This is
the first time I used this method and I run out of ideas how to
improve it.
Thanks in advance for your replies.
Dorota



From daemon Wed Mar 22 08:22:05 2000



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Wed, 22 Mar 2000 14:19:20 -0000
Subject: Accu-Edge Blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I have just caught up on some of the questions on this server and I think I
can contribute to the blade discussion. As far as I remember Accu-Edge
Blades were re-branded Feather blades sold by Anglia Scientific, a UK
microtome manufacturer in the 1970's and 80's. Anglia were taken over by
Shandon Scientific and in turn they all disappeared into Life Sciences
International. Phil Parker of Anglia I understand is still designing
microtomes for LSI.

In short for Accu-Edge read Feather. We supply these Feather blades as do
other microtome suppliers,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England

Tel ++44 118 981 7775 Fax ++44 118 981 7881



From daemon Wed Mar 22 08:32:11 2000



From: Cieslinski, Robert (RC) :      RCCIESLINSKI-at-dow.com
Date: Wed, 22 Mar 2000 09:21:18 -0500
Subject: Announcement - Michigan Local Affiliate Spring Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Call for Papers
Michigan Microscopy & Microanalysis Society
Spring Meeting

The Michigan Microscopy & Microanalysis Society (Local Affiliate of MSA)
will hold its Spring Meeting May 12th, 2000 at the Genoe Woods Conference
Center in Brighton, Michigan. If you are interest in presenting a microscopy
related paper or attending, please contact Deborah Rothe for more
information, email: drrothe1-at-dow.com .

The society encourages student participation by providing free meeting
registration for students whom present a paper. A cash award is sponsor by
the society vendors for the best student paper.


Robert C. Cieslinski
Michigan Microscopy & Microanalysis


Robert C. Cieslinski
The Dow Chemical Company
Microscopy & Microanalysis
(517) 636-6875
email: rccieslinski-at-dow.com



From daemon Wed Mar 22 18:12:00 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Wed, 22 Mar 2000 10:15:57 -0500
Subject: Re: Microscope Digital Cameras (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} The other Firewire camera I know if currently is the MicroImager.
} Check out http://www.qimaging.com/ Dave



}
} The only one I am aware of is the Optronics MagnaFire, which is a cooled
} camera with the IEEE-1394 FireWire interface.
}
} Previous versions of the Optronics cameras used a parallel (printer) port
} interface so they had to be used on PC's only. It would be worth inquiring
} about Mac compatibility. Sorry I don't have the answer to this part...I only
} know the MagnaFire has the FireWire interface.
}
} Visit {http://www.optronics.com} and click on "MagnaFire" for specs.
}
} Cheers,
}
} Bob Chiovetti

--

************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Wed Mar 22 18:12:01 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 07:23:13 -0600
Subject: Re: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would suggest that you look at UMAX Powerlook III. It is a flatbed scanner
with built in transparency adapter, so you can scan reflective and transmissive
media. Its resolution is 1200x2400 pixels. This should handle most anything
you might have. I use this scanner all the time. They are about $1100 these days.
For super high quality 35mm scanning, I use the Polaroid SprintScan 35+. It
does 2700 dpi, D=3.4. It runs about $1500. For medium format and 4x5"
transparent media, I use the Polaroid SprintScan 45. It runs about $4500 but
will handle 35mm and 6x6cm media.

I would not recommend the Nikon.

gary g.


At 07:48 PM 3/21/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Mar 22 18:12:01 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Wed, 22 Mar 2000 10:27:54 -0500
Subject: RE: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Valerie,
I can't comment on the Nikon scanner but I have plenty to say about the
Polaroid Sprintscan 45, most of which is probably best left unsaid! Let me
summarize. We purchased the Polaroid Sprintscan 45 in late 1998 and planned
to operate it on a PC with Windows 98 operating system. Our first unit was
defective and was replaced, however, the trouble shooting process was very
time consuming. Our second unit functioned intermittently. Again, after much
trouble shooting we returned the unit to Polaroid Canada. After having it
tested we were told that there were no problems with the Sprintscan 45. The
unit was returned to us but continued to cause us grief with its erratic
behavior. We spent more time trying to diagnose the problem and even called
in a computer specialist who spoke directly with Polaroid's tech support
personnel in an effort to resolve the problem. In the end we found that the
Sprintscan appears not to like Windows 98. The scanner has been running
sucessfully on an older PC with Windows 95. Why? Has there been an
identified problem associated with Windows 98? If so why aren't customers
alerted? Having this knowledge would have saved us an enormous amount of
time and money. I have been waiting for a comment from Polaroid for well
over a month.

I would be interested in hearing from other Sprintscan users who experienced
similar problems.

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
e-mail: paul.gerroir-at-crt.xerox.com

} -----Original Message-----
} From: Valerie Leppert [SMTP:vjleppert-at-ucdavis.edu]
} Sent: Tuesday, March 21, 2000 8:48 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Film Scanner Recommendations?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all-
}
} We are in the midst of writing a proposal for a new film scanner and I
} am hoping that someone out there can help with a recommendation for a top
} of the line film scanner. The major application would be TEM negatives. A
} model we are currently looking at is the Nikon LS-4500AF Multi Format Film
} Scanner.
}
} If anyone has archived recommendations from previous discussions and could
} send them on to me, that would be wonderful.
}
} Thank you,
} Valerie Leppert
}
} Dept. of Chem. Eng. and Mat. Sci.
} U. of California, Davis
}
} vjleppert-at-ucdavis.edu
}



From daemon Wed Mar 22 18:12:02 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Wed, 22 Mar 2000 10:28:05 -0500
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, there is a community of researchers involved in the use of EDS in
ESEM (mostly with the Electroscan now Phillips microscope but the work
is not exclusive of other low vacuum SEMs). See the Microscopy and
Microanalysis Meeting Proceedings from the past several years (in
1999-see Wight pg 290, Carlton pg 292). EDS spatial resolution can be
an issue depending on your conditions. Some general rules (to reduce
the scattering and therefore improve the EDS spatial resolution) are to
reduce the beam-gas-path length (shorten the distance the primary
electrons need to travel thru the gas molecules), lower the chamber
pressure (reduce the number of gas molecules in the path), use a less
scattering gas (Helium has already been suggested), and use as high an
accelerating voltage as reasonable. Several corrections have been
suggested for removing the unwanted contributions to the EDS spectrum:
the extrapolation method (already mentioned), beam stop method,
spectral subtraction method, and continuum method. There are also
simulation models that predict scattering by the gas, I am not aware of
any that predict EDS spectra based on a multiphase or multicomponent
system. I highly recommend that you take a typical sample and ask each
of the microscope manufacturers demonstrate imaging and EDS in their
scope.


I have no interest in any microscope or EDS system,
{fontfamily} {param} Times {/param} Certain commercial software, equipment,
instruments, or materials are identified in this report to specify
adequately the experimental procedure. Such identification does not
imply recommendation or endorsement by the National Institute of
Standards and Technology, nor does it imply that the materials or
equipment identified are necessarily the best available for the
purpose. {/fontfamily}

Scott


} ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

}

} It seems that nowadays every SEM vendor is offering variable pressure
models, which are conventional SEMs with a plumbing modification that
allows the sample to be at pressures of up to about 4 torr (500 Pa)
while the electron column operates at the conventional high vacuum. I
am very interested in buying a variable pressure SEM with an EDS, but
was recently warned that EDS has very poor spatial resolution in the
variable pressure mode because of the large beam spread due to
electrons scattering off the gas molecules in the sample chamber. Is
this really a significant issue? Do any of you have experience using
EDS with variable pressure SEMs?

} Thanks,



-------------------note: new mailing address----------------------

Scott Wight e-mail: scott.wight-at-nist.gov

NIST 222/A113 W voice: 301-975-3949

100 Bureau Dr STOP 8371 | fax: 301-417-1321

Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion
expressed is my own and does not represent those of my employer.


{/x-rich}



From daemon Wed Mar 22 18:12:02 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 22 Mar 2000 07:43:40 -0800
Subject: RE: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Valerie writes ...

} We are in the midst of writing a proposal for a new film
} scanner and I am hoping that someone out there can help
} with a recommendation for a top of the line film scanner.
} The major application would be TEM negatives. ...

I'll not knock the Nikon scanner, but I do believe your
ultimate choice should be based on trial. Most film scanners
anticipate normal films and normal exposures ... which addresses
the film's optical density (... defined as Dmax minus Dmin ...
approximately 3 f/stops for every OD unit ...). For normal
negatives this generally doesn't exceed '3', and for normal
positives, rarely exceeds '3.4'. However, the OD for x-ray
films and electron induced exposures are reported to approach
'4'. You should take one of your most problematic and
representative films and test the scanners, paying particular
attention to the detail/noise in the densest areas of the
negative.
I'll also mention ... you need not be limited to dedicated
film scanners. I don't recall the PPI resolution of the Nikon
scanner, but some of the newest flatbeds, together with their
transparency options, I'm sure can approach the the resolution
of the Nikon (e.g., 1600ppi for the new Epson) ... although I
would tend to believe the film scanners are the most likely to
deliver the best recognition for higher Dmax.

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - ICQ 210524
Geological Science's Electron Probe Facility - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/





From daemon Wed Mar 22 18:12:05 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 22 Mar 2000 10:21:28 -0600
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans,
Could you please explain the main idea of the
Vip-Quant algorithm. I cannot even imagine
how quantitative analysis could have nearly the
same accuracy as in high-vacuum mode without
a priori knowledge of the composition of
surrounding areas.
Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Tuesday, March 21, 2000 7:54 AM
} To: Everett Ramer
} Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Dear Everett,
}
} The extrapolation method (aka Variable Pressure Method)
} described by Dr.
} Bilde-Sorenson has been implemented by EDAX in a software
} feature called
} ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} conditions can
} be done with nearly the same accuracy as under High-Vacuum conditions.
} Although this method was developed with the ESEM microscope
} in mind, it of
} course can be applied to all Bad-Vacuum scanning electron microscopes.
}
} Please contact your local EDAX representative for more
} information and a
} copy of the new ViP-Quant brochure, or request one through
} www.edax.com.
}
} With best regards,
}
} Hans Dijkstra
}




From daemon Wed Mar 22 18:12:09 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 22 Mar 2000 11:27:43 -0600
Subject: Water purification system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are planning to upgrade our water supply from a reverse-osmosis,
deionization system by adding a "polishing" system. Since our volume
requirements are low, we're considering the Millipore Simplicity Ultrapure
Water System. Specs for this countertop unit are a resistivity of 18.2
megaohms-cm, with total organic content of {15 ppb for the product water.
The water would be used for routine EM use, i.e., making up reagants,
staining, immunolabeling, etc.

My question is: does anyone have any experience with these systems and would
you be willing to share it with us? Please feel free to respond off-line.

Thanks very much.
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Mar 22 18:12:11 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 22 Mar 2000 10:13:37 -0800 (PST)
Subject: RE: SprintScan+film scanner tips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a SprintScan 45, and while we don't work it to the bone, it has
been relatively reliable(We've had it serviced once since it was
purchased, and that was to have the internal motherboard replaced). I'm
sorry to hear that you've had so many problems Paul. We've had ours since
late 97, and it's been scanning consistently on a Win95 machine. It IS
very fussy with SCSI--let this be a warning to everyone. It won't work
with the new fast cards, but won't work with the really old cards either.
We've currently got it on a Adaptec AHA 15XX series SCSI2 card with UW
negotiation. We haven't been able to try it on a Win98 machine because all
of our Win98 machines have SCSI cards which are too fast.
I've heard good things about the Nikon(especially about Digital ICE).
Minolta also has Digital ICE. The SprintScan isn't perfect, but it gets
our job done. I would NOT recommend a flatbed with transparency adapter
for dedicated film scanning tasks--in general graphic arts professionals
agree that if you want to maximize scan quality from film, it's best to
get the dedicated film scanner rather than the multi-option flatbed.
Dedicated film scanners tend to have better bit depth too.

Of course, if money is no object, a drum scanner or an Imacon flextight
scanner will bring you the best images.

Disclaimer--I have no financial stake in any of the aforementioned
corporate entities blah blah blah

***
Pauline Yu
Microscopist Technician
USDA-ARS-WRRC



From daemon Wed Mar 22 18:12:13 2000



From: Seth Grotelueschen :      sethg-at-paxit.com
Date: Wed, 22 Mar 2000 12:59:12 -0600
Subject: Sony (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sony makes 2 firewire cameras, the DFW-V300 and DFW-V500. Both are c-mount
cameras and can be used on a light microscope. They are priced in a range
of $2000 or so.

Seth Grotelueschen
MIS, Inc.
www.paxit.com
sethg-at-paxit.com



-----Original Message-----



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 22 Mar 2000 13:20:29 -0600
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans,
Could you please explain the main idea of the
Vip-Quant algorithm. I cannot even imagine
how quantitative analysis could have nearly the
same accuracy as in high-vacuum mode without
a priori knowledge of the composition of
surrounding areas.
Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Tuesday, March 21, 2000 7:54 AM
} To: Everett Ramer
} Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Dear Everett,
}
} The extrapolation method (aka Variable Pressure Method)
} described by Dr.
} Bilde-Sorenson has been implemented by EDAX in a software
} feature called
} ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} conditions can
} be done with nearly the same accuracy as under High-Vacuum conditions.
} Although this method was developed with the ESEM microscope
} in mind, it of
} course can be applied to all Bad-Vacuum scanning electron microscopes.
}
} Please contact your local EDAX representative for more
} information and a
} copy of the new ViP-Quant brochure, or request one through
} www.edax.com.
}
} With best regards,
}
} Hans Dijkstra
}



From daemon Wed Mar 22 18:12:19 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Wed, 22 Mar 2000 14:43:39 -0600
Subject: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ya'll:
We are looking for a CCD camera for our Philips 430 TEM. We would like
to get the best camera for the best price, e.g., either buying a used
camera or a new non-Gatan camera (Gatan seems to be twice as expensive
as the others). We would be using the camera for bright field and high
resolution TEM of materials (semiconductors) rather than for biological
specimens. Does anyone have a camera they would like to sell/donate or
does anyone have recommendations as to a less-expensive camera that they
know can be used for materials applications.
Thanks, Mike Coviello
Lab Manager
University of Texas -at- Arlington



From daemon Wed Mar 22 18:12:21 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 22 Mar 2000 10:57:01 -1000 (HST)
Subject: diffusion pump contamination

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I probably have rotary pump oil in my oil vapor diffusion pump (long
story, situation remedied, I hope). My question is, how well do I have to
clean out the diffusion pump? Solvent clean and bake out, or only
wipe down? What are the probable effects of contamination? Will it affect
the vacuum quite a bit?

After overhauling the vacuum system on our Zeiss 10/A TEM because a faulty
anti-suction device allowed (a LOT) of r.p. oil to migrate throughout the
vacuum lines, I am only getting a vacuum of 6 X 10-4 when I should get 5 X
10-5. Either I've got a leak or a contaminated d.p. When I cleaned out
the d.p. I only wiped it out well, but did not wash it with solvent and
bake it out. I am trying to clean it in place rather than desolder the 13
wires (again) to get it all the way out of the scope, and then have to
resolder (again) while lying on my stomach in the dark recesses of the
instrument with glasses that are the wrong focal length... Call me lazy,
if you wish, but I'm trying to find the easiest but effective way to do
this!

Any tips and tricks appreciated!

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Mar 22 18:12:26 2000



From: David_Bell-at-Millipore.com
Date: Wed, 22 Mar 2000 16:09:00 -0500
Subject: Re: Water purification system

Contents Retrieved from Microscopy Listserver Archives
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Hi Randy,

No comment! :-)

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108



From daemon Wed Mar 22 18:12:27 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 13:40:03 -0600
Subject: RE: Film Scanner Recommendations? Sprintscan 45

Contents Retrieved from Microscopy Listserver Archives
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The Sprintscan 45; some people swear by them, others swear at them.

I swear by mine. But only after much trouble, and what sounds identical
to your experience. Here's what I found:

It really makes no difference as I see it whether one uses Win95 or Win98.
The crux of the problem is the SCSI controller and how it talks to the
scanner. The Sprintscan 35+ and the 45, the Agfa Arcus II, and several
other scanners were totally flaky and crash prone on my system. I too
sent back the Sprintscan 45 only to be told that it was fine. The SCSI
adapter is a key item in this whole picture. Which adapter were/are you
using?

At the time, I was running a FW SCSI system with an Adaptec AHA-2940UW.
The rear plate connector on this host adapter is a 68-pin FW connector.
The scanners are SCSI-I/II and are narrow, not wide....and definitely not fast.
So, in order to run a scanner on a FW connector, one needs a FW--} SCSI-I
cable and (this is the kicker) a high byte terminator on the host adapter
external connector and a standard narrow SCSI terminator at the scanner.
Good luck trying to get this high byte terminator. Adaptec has a part number
for it but I gave up trying to get one from them. Maybe you will be luckier
than I was.

The alternative is to connect to the narrow SCSI bus at the host adapter and
snake that out the rear of the PC using a blank panel interface that accepts
the 50-pin ribbon cable connector and presents a SCSI-II high density
connector at the rear of the computer. This can work and it did with the
Agfa scanner. But it did not work with the Sprintscans. Even when set
for 10MB/s and no negotiation, it still would hang the system. The solution?
I got a Mac G3/266. I still have it and I still use it for all scanning and
photo input.

Very interesting, and totally successful. All scanners work flawlessly on the
Mac's legacy external SCSI bus. I still am running FW SCSI inside for the
disks, but using the external SCSI for scanners, CD-R, CD-RW and 8mm
tape. The easiest way to run the scanners is via a common TWAIN
interface plug-in in Photoshop. My current main flatbed is a UMAX
Powerlook III. I have not tried it on my PC. My current PC is ATA-66
EIDE with an Adaptec AHA-2930 narrow SCSI adapter. It might run
the UMAX and the Polaroid but everything is hooked up to the Mac. With
DAVE on the Mac, all PCs, printers and the Mac talk to one another via TCP/IP
on a 10BaseT LAN. Any node that needs external access gets it via an
ISDN router. So there is no problem transporting files across platforms.
Worst case is a Zip disk.

Any Mac system before the G4 should work fine. The pre-G3 systems are
really cheap now. G3 systems can also be found rather inexpensively.
A simple system is all that one needs. Heavy duty computing is done on the
PC but photo input and scanning is done on the Mac. This solved all of
my problems--and from your discussion, they were the same as you experienced.

Hope this helps.

gary g.


At 09:27 AM 3/22/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Mar 22 18:12:28 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Mar 2000 13:48:40 -0800
Subject: TEM-shadow coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey,
I appreciate your feedback. It is obvious to me that you are pretty good at
this. First, I was unclear about the message to which I was responding. I
was responding to the subject "bacteriophage question" by way of Nestor.
To my limited knowledge, UA will positively stain proteins-UA is used as
a positive stain in thin section TEM. I understand the concept(s) of
negative staining well, and I appreciate that UA is one of the best negative
stains for high resolution work (small granularity+nice density).
Totally asymmetrical molecules may be 3-D reconstructed. It just takes
a brute force approach involving thousands of micrographs of the molecule of
interest randomly orientated in vitreous ice. Although your point about
symmetry makes good sense-a more symmetrical molecule should yield a higher
resolution reconstruction (require fewer micrographs for the same level of
confidence in a structure).
Your point about intrument limitations is also well recieved.
A quick concept: both TEM negative staining and Scanning Probe (AFM)
studies of protein macromolecules usually involve the use of charged
surfaces to facilitate adherance and/or orientation of the molecules (i.e.
carbon+glow discharge). Some feel that this may distort the shape of the
molecule (and indeed it will depending on the charge).
In the case of Scanning Probe, there are those who would say that the
"quasi-physical" interaction of the probe (presumably in tapping mode for
molecules under physiological conditions) distorts the reconstructed image
of the protein. I'ld like to say "sorry probe", but I don't have enough
confidence in these statements to do so.
Cheers,
Karl G.

----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 22, 2000 1:09 AM


Dear Dorota

You have to use such amount of platinum that it will create a small drop of
the melted metal at the end of the "V"-shape tungsten filament. If the
drop will be large, it is very likely that filament will broke. It seems
to me, 8 cm is a huge amount of platinum. Again, what is diameter of the
filament? 20 mA is a tiny current, seems to me, for thermal evaporation.
But, I never work on HUS 5GB, may be they have special setup. As for
granulation, I don't think that platinum will dramatically improve the
quality of your shadowing. Try platinum-palladium at least. May be, you
have to try buffers, which are able to vaporize in vacuum: ammonium acetate
or bicarbonate adjusted by CO2, for instance. For such applications I am
using for many years 50-150 mM ammonium acetate buffer (with some additives
if necessary, magnesium acetate, for instance) and tungsten shadowing by
electron gun. I also use thickness monitor to control shadowing. I also
use one or bi-directional shadowing: it resolves details better. I am
using rotary shadowing only for DNA and not in all cases. If I have any
problems with shadowing, I am using "test-object" - latex spheres to
determine the problem. Sometimes the problem is just wrong angle of
shadowing. If you have further questions, you may contact me off-listServer.

Sergey


} Date: Wed, 22 Mar 2000 10:13:39 -0400
} From: Dorota Wadowska {wadowska-at-upei.ca}
} Subject: TEM-shadow coating
} To: microscopy-at-sparc5.microscopy.com
} Organization: University of P.E.I.
} Priority: normal
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 22 18:12:32 2000



From: GANTZ-at-med-biophd.bu.edu
Date: Wed, 22 Mar 2000 17:31:48 -0400 (EDT)
Subject: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
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Our specimen was sprayed on to freshly cleaved mica, rotary
shadowed with platinum, and carbon coated. We are having difficulty
removing the carbon replica. Scoring around the edge did not help.
Would anyone have a suggestion? Thankyou.

Donald Gantz
Boston Univ School of Medicine
gantz-at-med-biophd.bu.edu



From daemon Wed Mar 22 18:12:33 2000



From: Stark,Margery :      Margery.Stark-at-ln.ssw.abbott.com
Date: Wed, 22 Mar 2000 16:45:28 -0600
Subject: Skeletal muscle fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,
Does anyone have a good method for skeletal muscle fixation for
Transmission Electron Microscopy? We will not be able to use a perfusion
method. A fixative method would also be greatly appreciated.

Many Thanks

Margery Stark
Abbott Laboratories
Department of Microscopy and Microanalysis
D-45M/AP31
200 Abbott Park Rd.
Abbott Park IL 60064-6202



From daemon Wed Mar 22 18:12:36 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Wed, 22 Mar 2000 17:19:40 -0600
Subject: RE: Buying a SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve Chapman writes:
} If you have a multi instrument laboratory I always feel
} that it is a good idea to have instruments from different manufacturers
} optimising the laboratory for optimising a wider range of applications.
}
Steve, You make some excellent points. However, I believe that, (just as
you state) potential benefits can be had by choosing different manufacturers
- at the same time you are missing some very significant opportunities if
you create a facility with many different manufacturers. Two important,
potential opportunities are: reduced training time and learning curves for
multiple users, and, discounted service contracts from the manufacturer.

For example, we have in the recent past had the benefit of significantly
reduced service contract costs by having multiple instruments from JEOL and
Philips. At one time our research facility here, happened to have four JEOL
EMs. These four SEMs covered three vastly different SEM instrumentation
ranges (and eras) and serviced different applications. They were all very
capable of handling the tasks for which they were purchased, and we were
able to save big bucks each year over the cost of single instrument service
contract prices. In addition we had multiple users who could go from one
instrument to another without any significant additional training (or
retraining) because of the relatively familiar user interface that
accompanied these microscopes.

Just another perspective.
Have Fun,
Brad Huggins

} ----------
} From: Steve Chapman[SMTP:PROTRAIN-at-CompuServe.COM]
} Sent: Wednesday, March 22, 2000 5:49 AM
} To: American EM Soc
} Subject: Buying a SEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} A few weeks ago, when our server was working correctly (*??!!*), there
} were
} e-mails talking about the purchase of a new SEM. Now we appear to be back
} on line I felt that our ideas may be of use to the mailers?
}
} As part of the consultancy side of our business we purchase SEM for our
} clients. This means we are buying instruments regularly and are therefore
} probably one of the few professional SEM purchasing organisations?
}
} In our team we have staff who have worked for most of the SEM
} manufacturers
} as demonstrators and application specialists, so we have seen the purchase
} game from both sides and of course try to use this to our clients
} advantage. Unlike the average microscopist, who probably only buys two
} instruments in their career, we have been able over the years to formulate
} a purchase plan, a purchasing criteria that we apply to each purchasing
} project. In our position we are unable to buy from the nicest salesman or
} the normal "gut" feeling, we must be absolutely certain that we are
} purchasing the best instrument for our clients applications.
}
} Set out below are the headings from a lecture that I give on instrument
} purchase. Only the basic ideas are presented ( I guess if you need more I
} shall be attacked by Microscopy Today to give just that) but you will get
} the theme. It is important to realise that the specimen chamber of a SEM,
} the specimen-detector geometry, determines what we will see. For this
} reason you will find that one instrument will do a particular task better
} than any other in the price range, you need test specimens that will sort
} this out for you. If you have a multi instrument laboratory I always feel
} that it is a good idea to have instruments from different manufacturers
} optimising the laboratory for optimising a wider range of applications.
}
} "Buying a New Microscope"
}
} How Do You Start?
}
} Collect ALL the brochures and prices
} Form a view of the new facilities being made available
} If you do not understand any features ASK the salesman
} Check through all the users desired facilities?
} Determine who will use the instrument now?
} Would there be others if you purchased particular accessories?
}
} Formulate a Purchase Specification
}
} Price range - we always look one level higher
} Set essential instrument specifications
} Set desired instrument specifications give them points and produce a
} "desirability assessment"
}
} Why use a consultant?
}
} Only he will without prejudice talk to all interested parties
} Interdepartmental feuds could spoil the case
} A different questioner provides different answers
} Their wide knowledge base may develop additional features
} They will have experience producing a "desirability assessment" which
} often
} brings surprises.
} They will be experienced with ways of dealing with the sales staff, they
} will keep them off your back
}
} How to handle the demonstration? DO NOT LET THEM
}
} Show you how wonderful the alignment procedures are - you will not spend
} all day aligning the instrument!
} Dominate YOUR demonstration
} Take you into totally uninteresting areas of the instrument - you should
} be
} buying because of the image quality
} Show their favourite gimmick
} Use their own favourite specimen
} Take you out of the room when about to load one of your specimens
} Take you to a two hour lunch with lots of drink
}
} How to handle the demonstration before you set off
}
} Select a maximum of three specimens that you know really well and that are
} important to your laboratory
} The specimens must be capable of meaningful low and high magnification
} imaging
} Develop a demonstration criteria for each specimen
} Have a tie break specimen available as suggested by the consultant
} Provide each company with an exact demonstration programme
}
} What a consultant would do during a demo
}
} Time the demonstrator to produce images at specific magnifications under
} very specific conditions
} Encourage the demonstrator (this is not a customer v demonstrator
} competition) allow them to try their own ideas with each specimen also,
} give them information that you have found to be of importance with your
} specimens
} Stop the demonstrator taking you away from your standard path - you do
} wish
} to compare each instrument under exactly the same conditions there is no
} time for other deviations
}
} After the demo
}
} Layout the results
} Compare performance instrument to instrument and time taken to obtain the
} results
} Produce a short list
} Compare the short list with the "desirability assessment"
} Research local and national service performance of the best two instrument
} manufacturers, and the manufacturers reputations
}
} Finally
} Decide upon the instrument that you want, the specification and then
} haggle
}
} Hope this helps?
}
} Steve Chapman
} Senior Consultant
} Protrain for Training and Consultancy in EM World Wide
} Tel & Fax 44+ 1280 814774
} e-mail protrain -at-emcourses.com
} web site www.emcourses.com
}



From daemon Wed Mar 22 18:12:37 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Mar 2000 15:26:31 -0800
Subject: Re: neg stain vs cryo EM plus image averaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Karl,
As my knowledge on thin sections, UA stains DNA and RNA, lead citrate -
some proteins and OSO4 - some lipids. Sometimes UA (as well as any others
stains) may just penetrate hydrophilic areas in the plastic sections (which
correspondent, usually, with biological material areas). In this case, I
agree, it may be named "positive staining". But in this case UA stain all
areas, which accessible to water, therefore it is not specific "positive"
stain for proteins.

As for 3D reconstruction. This is a subject for long and very interesting
discussion. I know just a few examples of the complete 3D reconstruction
of asymmetrical particles. Mostly, these works comes from Frank's (Spider
program) and Marin van Heel (Imagic program) groups. The resolution on it
is about 2 nm - not so impressive, if you count, that they did 3D on 26 nm
ribosome. Frank's algorithm with random tilted particles (on the support
film) has "dead angle" (between 60 and 90), therefore, the reconstruction
generally speaking is not "complete". Van Hell's algorithm supposed to be
free from such limitation, but it is not enough reconstructions performed
to prove this technique. But, I am very optimistic about that in general.
Both algorithms used "classification" of particles by type. Criteria for
such classification determined by operator, therefore it is possible that
in one class we will count slightly different particles (different shape or
different orientation or both), averaging of these particles will enhance
major features and lose fine details. Combination X-ray analysis and 3D
reconstruction data (for phasing of the X-ray data set at the beginning)
may help in this case. Marat Yusypov's grop did it for ribosome last year
with great success. But, I have to point, using X-ray crystallography you
studied some particular conformation under conditions of crystallization,
which is not necessary correctly reflect the real conformation in solution.

As for Scanning Probe Microscopy, I agree that probe may distort the
sample. To eliminate such problem, people scan the same area in different
directions. If the images are the same, it means, that distortion from
probe at least smaller than observed creatures. SPM may be very successful
for membrane proteins (which are difficult for EM), pore complexes, etc.
It is not necessary to use "modified" surfaces (glow-discharge, etc) for
SPM or "negative staining" (I never use it for NS, the secret is a quality
of the support carbon film). But, I agree with you, Karl that, as any
"microscopy", SPM is limited in some way in their abilities. Sorry, STM.

Karl, thank you so much for such nice discussion.

Sergey.


} Date: Wed, 22 Mar 2000 15:39:32 -0600
} From: Karl Garsha {keg-at-csd.uwm.edu}
} Subject: Re: neg stain vs cryo EM plus image averaging
} To: Microscopy-at-sparc5.microscopy.com, Sergey Ryazantsev {sryazant-at-ucla.edu}
} X-Mailer: Microsoft Outlook Express 5.00.2615.200
} X-MSMail-Priority: Normal
} X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200
}
} Dear Sergey,
} I appreciate your feedback. It is obvious to me that you are pretty good at
} this. First, I was unclear about the message to which I was responding. I
} was responding to the subject "bacteriophage question" by way of Nestor.
} To my limited knowledge, UA will positively stain proteins-UA is used as
} a positive stain in thin section TEM. I understand the concept(s) of
} negative staining well, and I appreciate that UA is one of the best negative
} stains for high resolution work (small granularity+nice density).
} Totally asymmetrical molecules may be 3-D reconstructed. It just takes
} a brute force approach involving thousands of micrographs of the molecule of
} interest randomly orientated in vitreous ice. Although your point about
} symmetry makes good sense-a more symmetrical molecule should yield a higher
} resolution reconstruction (require fewer micrographs for the same level of
} confidence in a structure).
} Your point about intrument limitations is also well recieved.
} A quick concept: both TEM negative staining and Scanning Probe (AFM)
} studies of protein macromolecules usually involve the use of charged
} surfaces to facilitate adherance and/or orientation of the molecules (i.e.
} carbon+glow discharge). Some feel that this may distort the shape of the
} molecule (and indeed it will depending on the charge).
} In the case of Scanning Probe, there are those who would say that the
} "quasi-physical" interaction of the probe (presumably in tapping mode for
} molecules under physiological conditions) distorts the reconstructed image
} of the protein. I'ld like to say "sorry probe", but I don't have enough
} confidence in these statements to do so.
} Cheers,
} Karl G.
}
} ----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, March 22, 2000 1:09 AM
} Subject: FW: neg stain vs cryo EM plus image averaging
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Karl
} } I disagree with you, that "UA in particular, will positively stain
} } proteins". Let start from definition: positively stain object is a dark
} } object on the light background (therefore stain penetrate/interact with
} } object making it "stained", dark in the terms of EM); "negative staining"
} } (NS) is opposite. We have light (stain do not interfere with the sample)
} } object on the dark background of stain (or dark ring of stain around the
} } object, depends from stain and technique). The beauty of the NS is that
} } stain (UA in particular) easily penetrates cavities in the object making
} } visible fine details (for instance, I was able to recognize variable and
} } constant domains of the Fab of the IgG molecules as well as domains of the
} } Fc using UA staining). May be this effect you call "positively staining".
} } But it is not.
} }
} } Yes, UA may stain RNA and DNA positively. For this reason UA gives us
} } "mixed stain" for ribosomes (negative for proteins and positive for RNA
} } components). The disadvantage UA is its low pH.
} }
} } As for subject of the current discussion. I think Cryo in general will
} } give us more correct information than others EM techniques. But, we have
} } to keep in mind, that in Cryo-technique, to make image relatively
} contrast,
} } people should go to very high overfocusing (from half to couple microns, I
} } believe). For this reason, the best Cryo-results I know, yielded only 2-3
} } nm resolution (and this is a huge problem in Cryo - to get better
} } resolution): the same or even worse as for "negative staining" (I expect
} } 1.5 nm resolution for UA). We have to count resolution when compare the
} } data. I lost the original message from which this discussion was started,
} } but it seems to me, that difference between NS and Cryo was not so
} dramatic
} } and may be comparable if we will count the resolution. In general, I
} don't
} } believe any EM data if resolution is not shown. Talking about resolution
} } is complicated because of the difference between "resolution of the
} } instrument" (0.14 nm for TEM currently), "physical resolution of the
} } method" (resolution limit for overfocusing, for instance, radiation
} damage,
} } drift, noise/signal ratio etc) and "resolution of the sample" (for
} } biological samples they are not the same: preparation of the sample may
} } affect sample's intactness/structure). Cryo is the best for sample
} } preservation, but it is low in contrast. UA may affect sample's structure
} } (may not) but the resolution limited only by granularity of stain (in
} } theory it is a couple angstroms).
} }
} } As for "averaging" of the images, I am a little bit skeptical about that.
} } It is great deal if your sample is uniform in shape (symmetry is a great
} } help too). If your biological sample is functionally flexible (IgG for
} } instance), averaging will "hide" some interesting details even if you will
} } distribute images in classes.
} }
} } In my point of view, it is difficult to extract absolute numbers from EM.
} } Inside one methodology you could easily compare the data, but it becomes
} } difficult when you want to compare the data obtained by different
} } techniques. This is reality of EM. I think Scanning Probe Microscopy is
} } very promising to obtain the direct measurements on the samples under
} } physiological conditions (EM is so far from "physiological conditions",
} } unfortunately). I am so sorry, EM!
} }
} } } Date: Mon, 20 Mar 2000 16:46:25 -0600
} } } From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
} } } Subject: FW: neg stain vs cryo EM plus image averaging
} } } To: microscopy-at-sparc5.microscopy.com
} } } X-Mailer: Microsoft Outlook Express for Macintosh - 4.01 (295)
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } }
} } } _____________________________________________________
} } }
} } } Karl E. Garsha, Doctoral Student
} } } AOP Fellow
} } } Chief Coordinator-Graduate Student Association
} } } Department of Biological Sciences
} } } University of Wisconsin-Milwaukee
} } } P.O. Box 413
} } } Milwaukee, WI 53201
} } } Office: 459 Lapham Hall
} } } Phone: (414) 229-4316
} } } Mobile: (414) 617-4295
} } } E-Mail: keg-at-csd.uwm.edu
} } }
} } } ----------
} } } } From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
} } } To: maxwell-at-lec.med.utoronto.ca
} } } Subject: neg stain vs cryo EM plus image averaging
} } } Date: Mon, Mar 20, 2000, 4:45 PM
} } }
} } }
} } } Dear Karen,
} } } My gut reaction: cryo-EM/3-D reconstruction will yield a much more
} accurate
} } } value with regard to measurement of macromolecular features.
} } } Some "negative" stains, UA in particular, will positively stain
} } } proteins, and so it would be expected that pore size might, in theory, be
} } } over-estimated in neg. stain images. This is a shortcoming of neg.
} staining
} } } as opposed to cryo-EM (if the protein isn't glycosylated). I have no
} doubt
} } } that investigators more experienced than I would argue this point
} however.
} } } The real power of 3-D reconstruction is that it averages many images
} and
} } } determines a structure that is statistically probable...the derived
} } } structure has a certain level of confidence. 3-D reconstruction, as well
} as
} } } 2-D averaging, may be performed on either neg. stained specimens
} (Wilkens,
} } } et. al., Journal of Biological Chemistry, 1999) or cryo-prepared
} specimens
} } } (many important citations...one of my favorites is Boisset, et. al.,
} Journal
} } } of Structural Biology 109, 39-45 (1992).
} } } Either way you need the computer software/hardware and theory
} (Journal
} } } of Structural Biology Vol. 116, Number 1, 1996).
} } } My main point is that a (responsibly) digitally enhanced image should
} } } provide more accurate measurement of macromolecular features than a raw
} } } negative stain photo.
} } } Best Regards,
} } } Karl G.
} } }
} } }
} } } _____________________________________________________
} } }
} } } Karl E. Garsha, Doctoral Student
} } } AOP Fellow
} } } Chief Coordinator-Graduate Student Association
} } } Department of Biological Sciences
} } } University of Wisconsin-Milwaukee
} } } P.O. Box 413
} } } Milwaukee, WI 53201
} } } Office: 459 Lapham Hall
} } } Phone: (414) 229-4316
} } } Mobile: (414) 617-4295
} } } E-Mail: keg-at-csd.uwm.edu
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
} }
} }
} }
} }
}
}
_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 22 18:12:39 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 16:15:36 -0600
Subject: Re: Microscope Digital Cameras (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thank you as well for your knowledge and insight, Dr. Ryazanstev. I've
enjoyed this discussion.
-Karl
----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 22, 2000 5:26 PM


At 05:59 PM 3/21/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It exists. But...and this is a big but....what performance do you seek?
Most of these, at least from what I have seen, are not industrial strength.
They are more consumer- than research-grade units. But maybe this
will satisfy your needs. The higher quality units tend to use SCSI from
what I have seen and used.

gary g.



From daemon Thu Mar 23 09:39:30 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 22 Mar 2000 20:07:57 -0500
Subject: TEM Pt/C replicas not releasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Donald Gantz wrote:
===============================================
Our specimen was sprayed on to freshly cleaved mica, rotary shadowed with
platinum, and carbon coated. We are having difficulty removing the carbon
replica. Scoring around the edge did not help. Would anyone have a
suggestion? Thankyou.
===============================================
There are probably as many different ways to approach this kind of problem
as there are people doing platinum replicas! The most reliable method we
have ever discovered is to use a 1% aqueous polyacrylic acid solution,
deposited as one drop on the surface with the stubborn replica. Twenty four
hours later, the drop has spread, the water has evaporated leaving a thin
but very tough PAA film. Then with something sharp (e. g. scalpel blade,
but wear eye protection for this!), slide the blade edge underneath the edge
of the PAA film, and if you have the "art", it will literally just "pop off"
. Now you have the tough PAA layer and the carbon coating, and where it
once rested on the mica, should now be a corresponding area free of replica.

Next the PAA is floated on a surface of water, carbon side up, and again, do
other things and come back 24 hours later. The PAA will have all dissolved
into the water, leaving the carbon/Pt film floating on the surface of the
water. The film is then picked up on grids as you would any other floating
film.

Note: Patience of clearly a virtue, be sure to give it the full 24 hours or
your grids will be PAA contaminated, with a major loss of contrast. Use a
deep petri dish for this so that there is sufficient volume of water to
efficiently dissolve the PAA.

If this sounds too complicated and you don't have patience, there is an
alternative we also use, it involves the use of Victawet® for EM (see our
URL
http://www.2spi.com/catalog/spec_prep/victawet.html

The Victawet is evaporated from a tungsten basket (see website instructions)
and a very thin layer of the release agent is deposited onto the mica (or
glass slide). Then apply your samples, shadow with Pt/C and the replica now
is almost guaranteed to float off on the first try.

One note: The "better" the grade of mica, I am told, the easier is the
release of such films. Grade V1 mica supposedly releases easier and that
might be because there are fewer cleavage steps. We have not tested that
theory ourselves so on that there can be no guarantees.....but it does make
sense. If you are not familiar with the different mica "grades", see URL
http://www.2spi.com/catalog/submat/chart.html

Disclaimer: SPI Supplies is a supplier of Victawet for Electron Microscopy
and mica substrate materials.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Thu Mar 23 09:39:32 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 17:21:14 -0600
Subject: Leaf Microlumina Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have one of these for sale. I may have two. These are specifically
for microscopy. At highest resolution, they take a 28MB TIF file.
This is as high of resolution I have ever seen. Interface is SCSI-II
small sized narrow-SCSI connector.



From daemon Thu Mar 23 09:39:46 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Mar 2000 20:44:54 -0800
Subject: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Donald

For platinum: try 1-10% aqueous HF. Use it instead water to float replica.
Insert mica slowly. Pickup replica on the grid and rinse with water. Use
corrosion-resistant tweezers. Do not immerse grids into HF solution if it
is copper. You have to do it fast to avoid corrosion of the grid. I was
using copper grids with carbon perforated (holey) film. Perhaps, carbon
protected grid form HF. This is easiest way for platinum shadowing.

You may use even 50% HF - it did not affect platinum shadowing as well as
carbon. Be careful, HF is extremely corrosive, avoid direct contact and
vapors.

If you have questions, you may contact to me off-line.

Good luck!

Sergey

} Date: Wed, 22 Mar 2000 17:31:48 -0400 (EDT)
} From: "GANTZ-at-med-biophd.bu.edu"-at-sparc5.microscopy.com
} Subject: Removal of Carbon Replica from Platinum-shadowed sample
} To: MICROSCOPY-at-sparc5.microscopy.com
} X-VMS-To: MICROSCOPY-at-MSA.MICROSCOPY.COM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Thu Mar 23 09:39:59 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 22 Mar 2000 22:01:15 -1000 (HST)
Subject: More about diff pump contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to thank those who have replied to my query about diff pump
contamination. Most have asked for more details...

How long have I let the scope pump after cleaning it out and changing diff
pump oil? Just shy of two weeks. The most improvement in vacuum came
within 4 days, then leveled off.

Do I think I got oil into the viewing chamber or column? Yeah,
lots. Lots. Most was on the anode plate, and quite a few droplets on the
liquid nitrogen anticontaminator plate. And even a drop hanging off the
filament! I can see all of you squirming... I cleaned the entire column,
including a couple of parts I had never been into before, and all the vac
lines. I was as thourough as possible, under the circumstances.

I presume I will have a fair amount of vapor around for years to
come. However, the scope operation and resolution aren't bad,
considering. The only thing that keeps me from slitting my wrist is that
we have this great, new, fancy LEO 912 EFTEM that I have been trying to
get all my users trained on, anyway. This means that the old Zeiss 10 can
be my hobby scope. It reminds me of the Volkswagens I used to work
on. Great German engineering!

So one friend thinks that small amounts of rotary pump oil in the
diffusion pump will "burn off" and cease to be a problem, one thinks that
it will only migrate up into the column, and a couple of third party
service people don't have a clue what to expect. No "official" word from
Zeiss/LEO yet. Hoping some of you clever microscopists will have either
direct knowledge or a grand theory backed by thoughtful physics!

Aloha,
Tina

Life's not all fun and Photoshop.
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Mar 23 09:40:01 2000



From: updancq3f1nkrz5xza-at-aqualung.knoware.nl
Date: Thu, 23 Mar 2000 04:49:05
Subject: Act Now - Reach Thousands of Prospects Monthly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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********************************************************
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From daemon Thu Mar 23 09:40:07 2000



From: redancq3f1nkrz5xza-at-molten.co.jp
Date: Thu, 23 Mar 2000 04:12:21
Subject: Special Offer - Reach Thousands of Prospects Monthly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

* * M A K I N G M O N E Y * *

How To Reach Thousands Of Prospects Every Month And Make The
Difference Between Success & Failure In Your Online Business

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

As they say, "It's the simple ideas that make money." And this
is SIMPLE & GREAT INCOME!

Our research has found that many people have tried one or more of
the following...

Free Classifieds? They just don't work anymore
Web Site? Takes thousands of surfers
Banners? Expensive and losing their punch
E-Zine? Hope they have a -huge- subscriber list
Search Engines? Forget it, unless you're in the top 20

S O W H A T D O E S W O R K ?

Although often misunderstood, there is one method that has proven
to succeed time-after-time.

E - M A I L M A R K E T I N G ! !

IT'S A FACT... It if you're not using your computer to generate
GOOD INCOME, you're leaving money on the table.

Here's what the experts have to say about E-Mail Marketing:

"A gold mine for those who can take advantage of
bulk e-mail programs" -The New York Times

"E-mail is an incredible lead generation tool"
-Crains Magazine

"Blows away traditional Mailing" -Advertising Age

Here's a potential earnings example: Let's say you have a
product or service that can bring a profit of around $30.
Remember, on the Internet, you can make money 7 days a week, 24
hours a day... even while you sleep, orders come from all over
the world!

Orders
Per Day Weekly Monthly Yearly

1 $ 210 $ 840 $ 10,080
2 420 1,680 20,160
3 630 2,520 30,240
5 1,050 4,200 50,400
10 2,100 8,400 100,000
15 3,150 12,600 151,200

The way to reach thousands of people, generate orders and build
wealth is person-to-person direct.

1. How do you find the millions of people on the Internet?

2. What are you going to tell them when you do reach them?


HERE'S THE ANSWER TO QUESTION #1

M I L L I O N S V O L U M E 9

***New - 10 Million addresses - Just Released***

The cleanest, most comprehensive e-mail address list in the
world! We're proud to offer it.

O N E O F A K I N D

This is a first. No one has gone to the work it takes to produce
an e-mail address list of this quality.

Here's how we prepare our e-mail lists:

1. We clean and eliminate all duplicates.

2. Next, we use a filter list of 400+ words/phrases to clean even
more. No address with inappropriate or profane wording survive!

3. Then we used our private database of thousands of known
"extremists", opposed to commercial e-mail, and kicked off every
one we could find.

4. And finally, we sorted the list into easy-to-manage
packets of 20,000 addresses in a simple text (.txt) format.

5. All domains have been verified as valid.

WHAT DID WE END UP WITH?

Volume 9... 10 Million Addresses Strong!

An address list so clean you'll want to start mailing today!


N O B R A G - J U S T F A C T

With our super clean e-mail address lists you'll send less...and
get better results...

* Y O U G E T W H A T Y O U P A Y F O R *

Our NEW 10 Million, Volume 9, address list CD will result in:

* Higher Response Rates
* Higher Sales Conversion Ratios
* More Receptive prospects; Less Flames & Non-Buyers.
* Less Contact With Anti-Commerce Radicals & Extremists.

Remember that potential income chart at the beginning of
this message? Can you imagine the kind of money you could
make if you mailed one million pieces and sold only one
tenth (.01%) of one percent? You do the math, you'll be
amazed!

We've been in the list brokerage business for over 5 years and
we've never compromised on quality. We won't release any address
list until it passes our "high standards" test.

This is not a rental list that is restricted to a one-time
mailing. You are purchasing an e-mail address list for your own
personal mailings and may use it over-and-over.

DON'T HESITATE on this offer or you will miss out on the least
expensive, legal and most effective way to market... PERIOD!

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

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From daemon Thu Mar 23 09:40:09 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Thu, 23 Mar 2000 10:13:53 +0000 (GMT Standard Time)
Subject: Re: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Donald,

This sounds very like the problem we had removing polymer single crystals
deposited on mica. The heavy metal tends to "key" to the mica, as it also
does if one is shadowing a bulk polymer specimen directly. What we do in
both cases is to put on a tiny amount of carbon first, about one-tenth of
the thickness of the main carbon film.

If the chemistry of your specimen allows, one might spray onto a
polystyrene surface, and then dissolve the PS with butanone.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+

On Wed, 22 Mar 2000 GANTZ-at-med-biophd.bu.edu-at-sparc5.microscopy.com wrote:

} Our specimen was sprayed on to freshly cleaved mica, rotary
} shadowed with platinum, and carbon coated. We are having difficulty
} removing the carbon replica. Scoring around the edge did not help.
} Would anyone have a suggestion? Thankyou.
}
} Donald Gantz
} Boston Univ School of Medicine
} gantz-at-med-biophd.bu.edu
}




From daemon Thu Mar 23 09:40:37 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 23 Mar 2000 21:52:36 +1000
Subject: RE: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


More chemical can access the replica if the replica is scored into 3mm squares
- which is required for TEM anyway. A backing of wax or plastic is useful if
the replica tends to fall apart - I don't expect that this would happen on a
mica surface.
For separating and cleaning use a spotting plate or a small watchglass within a
Petrie dish. I suggest to immerse the mica (or replicas) alternating in a
solution of chromic acid (made up from Pot dichromate) and then concentrated
household bleach - with a water rinse in between. Leave the mica and later the
specimen in those solutions for several hours or over night. 2 periods in both
solutions would be minimal for most tissues, but in your case, once the replica
has separated, the actual cleaning should be minimal.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 23, 2000 7:32 AM,
"GANTZ-at-med-biophd.bu.edu"-at-sparc5.microscopy.com
[SMTP:"GANTZ-at-med-biophd.bu.edu"-at-sparc5.microscopy.com] wrote:
}
} Our specimen was sprayed on to freshly cleaved mica, rotary
} shadowed with platinum, and carbon coated. We are having difficulty
} removing the carbon replica. Scoring around the edge did not help.
} Would anyone have a suggestion? Thankyou.
}
} Donald Gantz
} Boston Univ School of Medicine
} gantz-at-med-biophd.bu.edu



From daemon Thu Mar 23 09:40:39 2000



From: Harmon.Stephen-at-epamail.epa.gov
Date: Thu, 23 Mar 2000 08:50:15 -0500
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Ramer Everett,

Please see:

Doehne, E., A New Correction Method for High-Resolution Energy-Dispersive
X-ray Analyses in the Environmental Scanniing Electron Microscope. Scanning
1997, Vol. 19, 75-78.

Carlton, R. A., Energy Dispersive X-ray Spectrometry in the Environmental
Scanning Microscope. Microscope 1999, Vol. 47:1, 5-11.

Cheers,

Stephen M. Harmon
Electron Microscopist
United States Environmental Protection Agency
M.S. 681
26 W. Martin Luther King Blvd.
Cincinnati, OH 45268
513.569.7184
Harmon.Stephen-at-epa.gov




|--------+--------------------------}
| | Everett.Ramer-at-ne|
| | tl.doe.gov |
| | |
| | 03/20/2000 05:22|
| | PM |
| | |
|--------+--------------------------}
} ---------------------------------------------------------|
| |
| To: Microscopy-at-sparc5.microscopy.com |
| cc: |
| Subject: EDS in Variable Pressure SEM |
} ---------------------------------------------------------|






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


It seems that nowadays every SEM vendor is offering variable pressure models,
which are conventional SEMs with a plumbing modification that allows the sample
to be at pressures of up to about 4 torr (500 Pa) while the electron column
operates at the conventional high vacuum. I am very interested in buying a
variable pressure SEM with an EDS, but was recently warned that EDS has very
poor spatial resolution in the variable pressure mode because of the large beam
spread due to electrons scattering off the gas molecules in the sample chamber.
Is this really a significant issue? Do any of you have experience using EDS with
variable pressure SEMs?
Thanks,







From daemon Fri Mar 24 08:46:23 2000



From: koh young ho :      kohyh-at-bio.umass.edu
Date: Thu, 23 Mar 2000 11:25:46 -0500
Subject: buying ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I am interested in buying an ultramicrotome which is not necessary be a new
one. Does anybody have idea where I should contact?
Thank's in advances.
Sincerely,

Young Ho Koh
NSB program
UMass Amherst, MA 01003
kohyh-at-bio.umass.edu




From daemon Fri Mar 24 08:46:26 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 23 Mar 2000 13:17:15 -0400
Subject: Re: Skeletal muscle fixation

Contents Retrieved from Microscopy Listserver Archives
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At 4:45 PM -0600 3/22/0, Stark,Margery wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

**************************

I've worked with muscle (skeletal and cardiac) for years. If you can't
perfuse, you need to keep the muscles from contracting down on themselves.
Depending on the size of the muscle to be fixed, it can be handled as a
whole or blunt dissected into small (1-2mm diam) bundles and tied to
applicator sticks before immersing it in fix. It helps if you've boiled
the sticks in water for 10 minutes or so to extract any resins, etc. The
smaller the bundle, the better your initial fixation. You may want to wash
the bundles in bufer with "high" potassium (100mM KCl) to relax it. I fix
in 2.5% glut, 4% paraform. + approx. 0.02% picric acid (2 ml of a sat'd
sol'n in 40 ml of fix) in 0.1 M Na-cacodylate buffer(Ito & Karnovsky J Cell
Bio 89(abstr. 418) 1968). You can keep the 100mM KCl in this too. I would
suggest that you fix for 30 min or so at RT them overnight in the 'fridge.
Wash well, then cut into smaller pieces before osmium. dehydrate, etc as
usual. I like Spurr's resin, but feel free to use your favorite.

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Mar 24 08:46:26 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 23 Mar 2000 11:52:20 -0500
Subject: job openning - need immediately!

Contents Retrieved from Microscopy Listserver Archives
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ADVERTISEMENT

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Advanced Materials Processing and Analysis Center
(AMPAC)

The Advanced Materials Processing and Analysis Center (AMPAC) is seeking
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--------------------------------------------------------------------
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********************************************************************




From daemon Fri Mar 24 08:46:29 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Thu, 23 Mar 2000 12:10:38 -0600
Subject: TEM-Digital camera recommendations-an apology

Contents Retrieved from Microscopy Listserver Archives
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Hi Ya''ll:
In reference to my previous message:
I'm sorry I might have given the impression that I feel the Gatan
digital camera is overpriced...We have lots of Gatan equipment and are
extremely happy with it. Maybe I should have said: we would like to
know what are the lower-priced options for a CCD camera for a Philips
430 (without singling out Gatan). I am sorry for any offense this may
have caused.
Regards, Mike Coviello
University of Texas -at- Arlington



From daemon Fri Mar 24 08:46:29 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Thu, 23 Mar 2000 19:19:11 +0100
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Vladimir,

The main idea of the ViP-Quant procedure is rather straight-forward and
elegant. It is based on the phenomenon that as the pressure increases the
size of the skirt effect remains fairly constant, although the number of
skirt electrons increases, and thus the contribution of the skirt-effect to
the EDS signal increases. This intensity increase is linear with the
pressure.

This is valid for low- to medium pressures, or at a higher (ESEM) pressure
with a very short free-path length of the skirt electrons, since in both
cases you can assume the skirt electrons have scattered only once. At higher
pressures with longer working distances these assumptions are no longer
fully valid.

So to do an EDS analysis you take 2 measurements at different pressures, the
low-pressure one at a pressure where you can just avaid charging, and a
high-pressure one at at least twice that pressure. The measured intensities
of all elements are then plotted as a function of pressure, and extrapolated
to pressure zero, i.e. high vacuum. The extrapolated results will be very
close to the results hat would have been obtained directly if the sample
could have been analyzed at high vacuum conditions.

Of course anyone can do this plotting and extrapolation manually, that is:
if your EDS software allows you to enter intensities manually! The only
thing the EDAX ViP-Quant is offering as an extra is that the software does
it automatically for you. There are no proprietary miracles involved, EDAX
has just listened carefully to what science has to offer....

Best regards,

Hans Dijkstra

Disclaimer: The above is my personal opinion, and not necessarily EDAX's.
-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
}
} From: "Dusevich, Vladimir", INTERNET:DusevichV-at-umkc.edu
} To: Everett Ramer, INTERNET:Everett.Ramer-at-netl.doe.gov
} "'Hans Dijkstra'", INTERNET:hans.dijkstra-at-edax.nl
}
} CC: Joergen Bilde-Soerensen 5802, INTERNET:j.bilde-at-risoe.dk
} [unknown], INTERNET:Microscopy-at-sparc5.microscopy.com
}
} Date: 3/22/00 4:35 PM
}
} RE: RE: EDS in Variable Pressure SEM
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hans,
} Could you please explain the main idea of the
} Vip-Quant algorithm. I cannot even imagine
} how quantitative analysis could have nearly the
} same accuracy as in high-vacuum mode without
} a priori knowledge of the composition of
} surrounding areas.
} Thank you,
}
} Vladimir
}
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
} } -----Original Message-----
} } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } Sent: Tuesday, March 21, 2000 7:54 AM
} } To: Everett Ramer
} } Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} } Subject: RE: EDS in Variable Pressure SEM
} }
} }
} } Dear Everett,
} }
} } The extrapolation method (aka Variable Pressure Method)
} } described by Dr.
} } Bilde-Sorenson has been implemented by EDAX in a software
} } feature called
} } ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} } conditions can
} } be done with nearly the same accuracy as under High-Vacuum conditions.
} } Although this method was developed with the ESEM microscope
} } in mind, it of
} } course can be applied to all Bad-Vacuum scanning electron microscopes.
} }
} } Please contact your local EDAX representative for more
} } information and a
} } copy of the new ViP-Quant brochure, or request one through
} } www.edax.com.
} }
} } With best regards,
} }
} } Hans Dijkstra
} }
}
}
}
}
}
}
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} From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} To: "'Hans Dijkstra'" {hans.dijkstra-at-edax.nl} ,
} Everett Ramer
} {Everett.Ramer-at-netl.doe.gov}
} Cc: Microscopy-at-sparc5.microscopy.com,
} Joergen Bilde-Soerensen 5802
} {j.bilde-at-risoe.dk}
} Subject: RE: EDS in Variable Pressure SEM
} Date: Wed, 22 Mar 2000 10:21:28 -0600
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From daemon Fri Mar 24 08:46:30 2000



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Thu, 23 Mar 2000 10:37:55 -0800
Subject: Re: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
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Donald -

I sometimes found this and never had a great explanation of why it
happened. I would make sure that your bell jar is clean, i.e.free from
oil. I did find that the thickness of the carbon sometimes mattered. If
it was too thick it shattered. Too thin you cannot see it or it won't stay
together. Sometimes in between it would not float off.

Good luck.

ML
}
}
} Our specimen was sprayed on to freshly cleaved mica, rotary
} shadowed with platinum, and carbon coated. We are having difficulty
} removing the carbon replica. Scoring around the edge did not help.
} Would anyone have a suggestion? Thankyou.
}
} Donald Gantz
} Boston Univ School of Medicine
} gantz-at-med-biophd.bu.edu

Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu



From daemon Fri Mar 24 08:46:30 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 23 Mar 2000 15:19:48 -0400
Subject: Re: buying ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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At 11:25 AM -0500 3/23/0, koh young ho wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*****************
for used instruments in the NY Metropoitan are you can try:
M.O.C. at (914)268-6450
and
Marcus Meyerhoff (I'lm not sure I've spelled that correctly) at (732) 747-6228

Lee




From daemon Fri Mar 24 08:46:31 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 23 Mar 2000 14:16:52 -0500
Subject: RE: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
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Subject: RE: Film Scanner Recommendations?

My name is George Laing from National Graphic Supply. NGS is a vendor of
electronic imaging and traditional photographic products to scientific
markets.
Our customers have had excellent results scanning TEM negatives with the
Agfa Duoscan T2500 scanner. The T2500 looks like a traditional "flatbed"
scanner but utilizes a unique "Twin Plate" design similar to a negative
carrier, that eliminates the use of glass in the film holder. This
eliminates Newton rings,dust on the scanner glass, etc.
Optical resolution is 2500x2500ppi(maximum resolution 5000x5000), Dmax is
3.5. The T2500 has Apochromatic optics and also includes glassless film
holders for 35mm, 120/220 and 4x5 films. Also included is a glass drawer
for transparent originals up to 8"x10". Agfa's Fotolook software is
included for Mac and Windows, interface is SCSI. In addition, the T2500
will also scan reflective originals up to 8.5" x 14".
There are three models in the Duoscan family ranging from
$750 to $5175 list price.
I can provide sample output and literature for anyone who wishes to
contact me directly or visit
http://www.agfahome.com/product/CatProd_DisplayPublic.html?id=3115

George Laing
National Graphic Supply
E-mail: scisales-at-ngscorp.com
Phone: (800) 223-7130 X3109 USA



Valerie,
I can't comment on the Nikon scanner but I have plenty to say about the
Polaroid Sprintscan 45, most of which is probably best left unsaid! Let me
summarize. We purchased the Polaroid Sprintscan 45 in late 1998 and planned
to operate it on a PC with Windows 98 operating system. Our first unit was
defective and was replaced, however, the trouble shooting process was very
time consuming. Our second unit functioned intermittently. Again, after much
trouble shooting we returned the unit to Polaroid Canada. After having it
tested we were told that there were no problems with the Sprintscan 45. The
unit was returned to us but continued to cause us grief with its erratic
behavior. We spent more time trying to diagnose the problem and even called
in a computer specialist who spoke directly with Polaroid's tech support
personnel in an effort to resolve the problem. In the end we found that the
Sprintscan appears not to like Windows 98. The scanner has been running
sucessfully on an older PC with Windows 95. Why? Has there been an
identified problem associated with Windows 98? If so why aren't customers
alerted? Having this knowledge would have saved us an enormous amount of
time and money. I have been waiting for a comment from Polaroid for well
over a month.

I would be interested in hearing from other Sprintscan users who experienced
similar problems.

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
e-mail: paul.gerroir-at-crt.xerox.com

} -----Original Message-----
} From: Valerie Leppert [SMTP:vjleppert-at-ucdavis.edu]
} Sent: Tuesday, March 21, 2000 8:48 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Film Scanner Recommendations?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all-
}
} We are in the midst of writing a proposal for a new film scanner and I
} am hoping that someone out there can help with a recommendation for a top
} of the line film scanner. The major application would be TEM negatives. A
} model we are currently looking at is the Nikon LS-4500AF Multi Format Film
} Scanner.
}
} If anyone has archived recommendations from previous discussions and could
} send them on to me, that would be wonderful.
}
} Thank you,
} Valerie Leppert
}
} Dept. of Chem. Eng. and Mat. Sci.
} U. of California, Davis
}
} vjleppert-at-ucdavis.edu
}




From daemon Fri Mar 24 08:46:36 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 23 Mar 2000 11:56:04 -0800 (PST)
Subject: video microscope attachments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello,
I was wondering if I could get a sampling of opinions on the different
attachments to light microscopes, dissecting microscopes to display video
to an external monitor. I've seen a few different products available, and
would like to know any experiences people may have had with different
systems. Any comments are greatly appreciated, and I will happily make a
summary of the information.

Sincerely,
Gordon Vrdoljak.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Mar 24 08:46:38 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 23 Mar 00 14:10:03 -0600
Subject: Re: Extracting Precipitates With Replicas

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The ongoing discussion about replica techniques continues to be
very
educational. I wish to suggest another approach to this analysis
problem.
Occasionally I jet polish foils of metals which contain precipitates.
Certain
acid mixtures seem more "agressive" and preferentially remove the
precipitates. This seems prone to happen when using nitric or perchloric
acid mixtures.
However, a few years ago when I began to use "non-acid"
electrolytes,
precipitates were sometimes thinned beautifully and were still retained
in the thinned foil. I once got a hole in the center of a large
precipitate!
For more information, see Ultramicroscopy, Vol.19,(1986).
Perhaps more research should be funded along this "thread".
A second approach has been to "design" less agressive electrolytes
that rely on hydrochloric acid, for example, which sometimes works for
some materials but is not in The general literature. Within safty
considerations,worthwhile electrolyte improvements may be achieved with a bit of
work! Good luck.

Bernie Kestel
Materials Science Division
Argonne National Laboratory
9700 South Cass Avenue
Argonne, Il., 60439

E-mail: {kestel-at-anl.gov} FAX: (630) 252-4289







From daemon Fri Mar 24 08:46:53 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 23 Mar 2000 15:08:33 -0600
Subject: RE: EDS in Variable Pressure SEM

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"Dusevich, Vladimir"
{DusevichV-at-umkc.edu}
Cc: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}


Hans,
Thank you very much for explanations.
But for me this method will not work -
quite often I analyze wet specimens at
pretty high pressure (5-6 Torr).
Thank you again,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Thursday, March 23, 2000 12:19 PM
} To: DusevichV-at-umkc.edu
} Cc: Microscopy Listserver
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Dear Vladimir,
}
} The main idea of the ViP-Quant procedure is rather
} straight-forward and
} elegant. It is based on the phenomenon that as the pressure
} increases the
} size of the skirt effect remains fairly constant, although
} the number of
} skirt electrons increases, and thus the contribution of the
} skirt-effect to
} the EDS signal increases. This intensity increase is linear with the
} pressure.
}
} This is valid for low- to medium pressures, or at a higher
} (ESEM) pressure
} with a very short free-path length of the skirt electrons,
} since in both
} cases you can assume the skirt electrons have scattered only
} once. At higher
} pressures with longer working distances these assumptions are
} no longer
} fully valid.
}
} So to do an EDS analysis you take 2 measurements at different
} pressures, the
} low-pressure one at a pressure where you can just avaid
} charging, and a
} high-pressure one at at least twice that pressure. The
} measured intensities
} of all elements are then plotted as a function of pressure,
} and extrapolated
} to pressure zero, i.e. high vacuum. The extrapolated results
} will be very
} close to the results hat would have been obtained directly if
} the sample
} could have been analyzed at high vacuum conditions.
}
} Of course anyone can do this plotting and extrapolation
} manually, that is:
} if your EDS software allows you to enter intensities
} manually! The only
} thing the EDAX ViP-Quant is offering as an extra is that the
} software does
} it automatically for you. There are no proprietary miracles
} involved, EDAX
} has just listened carefully to what science has to offer....
}
} Best regards,
}
} Hans Dijkstra
}
} Disclaimer: The above is my personal opinion, and not
} necessarily EDAX's.
} -------------------------------------------------------------
} EDAX Europe www.edax.com
} Ringbaan Noord 103 Tel.: +31-13-5364000
} P.O. Box 4144 Fax.: +31-13-5356279
} 5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
} the Netherlands
} -------------------------------------------------------------
}
} } -----Original Message-----
} }
} } From: "Dusevich, Vladimir", INTERNET:DusevichV-at-umkc.edu
} } To: Everett Ramer, INTERNET:Everett.Ramer-at-netl.doe.gov
} } "'Hans Dijkstra'", INTERNET:hans.dijkstra-at-edax.nl
} }
} } CC: Joergen Bilde-Soerensen 5802, INTERNET:j.bilde-at-risoe.dk
} } [unknown], INTERNET:Microscopy-at-sparc5.microscopy.com
} }
} } Date: 3/22/00 4:35 PM
} }
} } RE: RE: EDS in Variable Pressure SEM
} }
} }
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} ---------.
} }
} }
} } Hans,
} } Could you please explain the main idea of the
} } Vip-Quant algorithm. I cannot even imagine
} } how quantitative analysis could have nearly the
} } same accuracy as in high-vacuum mode without
} } a priori knowledge of the composition of
} } surrounding areas.
} } Thank you,
} }
} } Vladimir
} }
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 3127 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } Phone: (816) 235-2072
} } Fax: (816) 235-5524
} } Web: http://www.umkc.edu/dentistry/microscopy
} }
} } } -----Original Message-----
} } } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } } Sent: Tuesday, March 21, 2000 7:54 AM
} } } To: Everett Ramer
} } } Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} } } Subject: RE: EDS in Variable Pressure SEM
} } }
} } }
} } } Dear Everett,
} } }
} } } The extrapolation method (aka Variable Pressure Method)
} } } described by Dr.
} } } Bilde-Sorenson has been implemented by EDAX in a software
} } } feature called
} } } ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} } } conditions can
} } } be done with nearly the same accuracy as under
} High-Vacuum conditions.
} } } Although this method was developed with the ESEM microscope
} } } in mind, it of
} } } course can be applied to all Bad-Vacuum scanning electron
} microscopes.
} } }
} } } Please contact your local EDAX representative for more
} } } information and a
} } } copy of the new ViP-Quant brochure, or request one through
} } } www.edax.com.
} } }
} } } With best regards,
} } }
} } } Hans Dijkstra
} } }
} }
} }
} }
} }
} }
} }
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} } Message-ID: {95A711A70065D111B58C00609451555C01CA7552-at-UMKC-MAIL02}
} } From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} } To: "'Hans Dijkstra'" {hans.dijkstra-at-edax.nl} ,
} } Everett Ramer
} } {Everett.Ramer-at-netl.doe.gov}
} } Cc: Microscopy-at-sparc5.microscopy.com,
} } Joergen Bilde-Soerensen 5802
} } {j.bilde-at-risoe.dk}
} } Subject: RE: EDS in Variable Pressure SEM
} } Date: Wed, 22 Mar 2000 10:21:28 -0600
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From daemon Fri Mar 24 08:46:54 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Thu, 23 Mar 2000 13:19:43 -0800
Subject: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I've heard from several SEM/TEM independent maintenance companies that
some of the manufacturer's have engaged in "price dumping" when
negotiating a service contract.

In other words, if the manufacturer is aware that they have competition

for a service contract they will reduce the contract by as much as forty

percent.

I am all for the free enterprise system, but I thought that when a
contract price is offered it is the same for all customers especially
government contracts that must abide by the GSA rules. GSA rules states

that the price offered is the lowest price for this service. It would
be
illegal for the manufacturer (or anyone) to offer a contract at less
than the cost offered to GSA customers.

I am surprised by this move as our contracts are the same as we abide
by
the GSA rules.

Regards,

Earl Weltmer



From daemon Fri Mar 24 08:46:55 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 23 Mar 2000 15:43:53 -0600
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EDS in ESEM is a pretty similar thing to
EDS in SEM if you do not need trace/minor (~1%) element analysis
and use some standard precautions. Some vendors even
sell as an option gaseous detectors which could be
placed close (1mm) to a sample and greatly reduce
a beam scattering. I routinely use EDS at water vapor
pressure up to 6 Torr. I've put an X-ray map taken
in environmental conditions at our web site
http://www.umkc.edu/dentistry/microscopy
If low concentrations are of no interest to you then
you will not see real difference in performance of an
EDS in VP and high vacuum modes, especially if you
do not work with wet specimens which need really high
pressure.


Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

}
}
} It seems that nowadays every SEM vendor is offering variable
} pressure models, which are conventional SEMs with a plumbing
} modification that allows the sample to be at pressures of up
} to about 4 torr (500 Pa) while the electron column operates
} at the conventional high vacuum. I am very interested in
} buying a variable pressure SEM with an EDS, but was recently
} warned that EDS has very poor spatial resolution in the
} variable pressure mode because of the large beam spread due
} to electrons scattering off the gas molecules in the sample
} chamber. Is this really a significant issue? Do any of you
} have experience using EDS with variable pressure SEMs?
} Thanks,
}
}



From daemon Fri Mar 24 08:46:57 2000



From: Scott Robinson :      sjrobin-at-itg.uiuc.edu
Date: Thu, 23 Mar 2000 16:16:36 -0600
Subject: SEM query: aquatic invertebrates

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Some of the K-12 classrooms we serve in our Bugscope
(http://bugscope.beckman.uiuc.edu/) program have expressed interest in
sending us aquatic or marine invertebrates to prepare for their sessions.
What I'd like to know is how I should ask them to prepare the samples for
me (e.g., should I ask them to overnight pondwater or seawater samples in
plastic bottles?), and after that, what should I do with them? I can't
really be sending glutaraldehyde and cacodylate to gradeschool kids, but
I'm assuming I'll want to do some sort of standard
fixation/dehydration/HMDS treatment, presumably on Millipore filters, once
I get samples. Any tips?

Thanks
Scott Robinson




From daemon Fri Mar 24 08:47:02 2000



From: Tom Gore :      togo-at-uvvm.uvic.ca
Date: Thu, 23 Mar 2000 14:54:39 -0800
Subject: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
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} We are in the midst of writing a proposal for a new film scanner and I
} am hoping that someone out there can help with a recommendation for a top
} of the line film scanner. The major application would be TEM negatives. A
} model we are currently looking at is the Nikon LS-4500AF Multi Format Film
} Scanner.
}
} Valerie Leppert
} Dept. of Chem. Eng. and Mat. Sci.
} U. of California, Davis

We use a Polaroid SprintScan 45 for SEM, TEM and other large film but find
that the negatives need to be somewhat tailored to the scanner's needs. We
have great difficulty with very dense or very high contrast negatives,
especially from the TEM. Staining and microscope settings need to be
adjusted to produce negatives that are within the range of the scanner. It
then produces very good results.


_____________________________________
Tom Gore, Advanced Imaging Laboratory
Biology Department, University of Victoria
Box 3020, Station CSC,
Victoria, BC, V8W 3N5 Canada
voice (250) 721-7134 fax (250) 721-7120
web: http://web.uvic.ca/ail/




From daemon Fri Mar 24 08:47:05 2000



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Thu, 23 Mar 2000 17:40:29 -0600
Subject: SEM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for an independant service provider for our JEOL-880 SEM.
They would need to be very familiar with JEOL equipment since the 880 is
a rare bird (the only one in the States, I believe). I believe it's a
1200 TEM column married to 840 electronics. We are located in central
Oklahoma. TIA.

Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Fri Mar 24 08:47:09 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 23 Mar 2000 18:21:34 -0600
Subject: The Image Processing Tool Kit

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a vendor to purchase The Image Processing Tool Kit
by John Russ. I placed an order with Amazon nearly a month ago but
have yet to receive anything. Hmmmm.

Thank you,

John B.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Mar 24 08:47:13 2000



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Fri, 24 Mar 2000 09:11:27 +0800
Subject: RE: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
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Mike,

You could check out Soft Imaging Systems' MegaView II at:

http://www.soft-imaging-web.de/ {http://www.soft-imaging-web.de/}

I'd like to hear other recommendations too...

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260

TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg {mailto:m-yeadon-at-imre.org.sg}



-----Original Message-----
From: Michael Coviello [SMTP:coviello-at-mae.uta.edu]
Sent: Thursday, March 23, 2000 4:44 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: TEM-Digital camera recommendations


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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Hi Ya'll:
We are looking for a CCD camera for our Philips 430 TEM. We would
like
to get the best camera for the best price, e.g., either buying a
used
camera or a new non-Gatan camera (Gatan seems to be twice as
expensive
as the others). We would be using the camera for bright field and
high
resolution TEM of materials (semiconductors) rather than for
biological
specimens. Does anyone have a camera they would like to sell/donate
or
does anyone have recommendations as to a less-expensive camera that
they
know can be used for materials applications.
Thanks, Mike Coviello
Lab Manager
University of Texas -at- Arlington




From daemon Fri Mar 24 08:47:14 2000



From: Susnitzky, David :      david.susnitzky-at-intel.com
Date: Thu, 23 Mar 2000 17:16:49 -0800
Subject: Announcement - N. California Local Affiliate Meeting on March 30t

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N. California Society for Microcsopy

MARCH 30TH MEETING AT GENENTECH
TOPIC: Microscopy and Public Health.

Our two speakers come from the USDA, Agricultural Research Service in
Albany, CA. Robert Mandrell's presentation is titled "Analysis of Human
Pathogens on Food Surfaces by Stereo- and Confocal Micros-copy". Robert is
the Research Leader of the Food Safety and Health Unit at the USDA facility.
Also speaking is De Wood on "Immunolocalization using FESEM and a
Backscatter Detector". De heads up the Microscopy & Imaging Lab at the
USDA.

Please call Greg Lum at 415/338-1339 or Email: "glum-at-sfsu.edu" for dinner
reservations and entree choice by Monday March 27th. The meeting starts at
5:00 p.m. with a beverage reception. The dinner will start at 6:00 pm.

DINNER RESERVATIONS
Entree choice (select one) $20 nonmember, $15 regular members; $8
student members
Menu choices for Genentech meeting on March 30th
[ ] Chicken Parmesan
[ ] Flank Steak with Mushroom Sauce
[ ] Pasta Primavera

Please call Greg Lum at 415/338-1339 or Email: "glum-at-sfsu.edu" for dinner
reservations and entree choice by Monday March 27th. The meeting starts at
5:00 p.m. with a beverage reception. The dinner will start at 6:00 pm.



From daemon Fri Mar 24 08:47:18 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 23 Mar 2000 17:52:30 -0800 (PST)
Subject: Re: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are two labs on campus with the Agfa Duoscan T2500 scanners. As
stated in the message from National Graphics Supply, they have both a flat
bed and a transparency drawer. They will scan at 16-bit gray scale as
well as in 8-bit and RGB. Weve been very happy with ours, primarily using
it for TEM and SEM negatives, and for 35 mm. One caveat, the 2500 dpi
capability is limited to a 4 inch x 14 inch area (1/2 width of the scan
area). Set-up was simple and no crashes (so far). The software is easy to
learn with identical interfaces for both the standalone application and
the Photoshop compatible plug-in.

We purchased ours locally from a professional photography retailer.

Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu




From daemon Fri Mar 24 08:47:21 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 23 Mar 2000 18:17:31 -0600
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hum.... This is very interesting. Government customers are one
thing, non-government customers are another. GSA rules and
principles do not apply to non-government customers. If you
would please cite the FAR that is the basis for your assertion,
this could help a lot to clarify your statement.

I have never heard of "price dumping." But I have heard of
competition. One must keep U.S. government business separate
from others. Even then, how does one mingle them together?
On what basis is this done?

How much of this "competition" is based on multiple unit discounts versus
actual price reduction? And as a consumer, what real difference
does it make? If the consumer can negotiate a good deal, all
the better for the consumer, right?

Mixing GSA into free enterprise is like apples and oranges, so
to speak.

What do you think?

gary g.


At 03:19 PM 3/23/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 24 08:47:26 2000



From: earlw-at-pacbell.net
Date: Thu, 23 Mar 2000 19:22:06 -0800
Subject: Re: SEM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You are right. The 880 is an "immersion lens" SEM, a very rare animal. There
are only two that I know of: One at OU, the second was at IBM in France but
is now in the back of my office sadly used for parts.

Earl Weltmer

Bill Chissoe wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are looking for an independant service provider for our JEOL-880 SEM.
} They would need to be very familiar with JEOL equipment since the 880 is
} a rare bird (the only one in the States, I believe). I believe it's a
} 1200 TEM column married to 840 electronics. We are located in central
} Oklahoma. TIA.
}
} Bill
}
} --
} =============================================================
} Bill Chissoe III
} Electron Microscopist
} University of Oklahoma
} 770 Van Vleet Oval
} Norman, Ok. 73019
} E-mail: wchiss-at-ou.edu Ph. (405)325-4391
} =============================================================



From daemon Fri Mar 24 08:47:27 2000



From: earlw-at-pacbell.net
Date: Thu, 23 Mar 2000 19:23:04 -0800
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Company providing the service does certify to the GSA customer that the
prices given are the same or lower than the prices given to any other entity:
public, government or private.

Earl Weltmer

"Dr. Gary Gaugler" wrote:

} Hum.... This is very interesting. Government customers are one
} thing, non-government customers are another. GSA rules and
} principles do not apply to non-government customers. If you
} would please cite the FAR that is the basis for your assertion,
} this could help a lot to clarify your statement.
}
} I have never heard of "price dumping." But I have heard of
} competition. One must keep U.S. government business separate
} from others. Even then, how does one mingle them together?
} On what basis is this done?
}
} How much of this "competition" is based on multiple unit discounts versus
} actual price reduction? And as a consumer, what real difference
} does it make? If the consumer can negotiate a good deal, all
} the better for the consumer, right?
}
} Mixing GSA into free enterprise is like apples and oranges, so
} to speak.
}
} What do you think?
}
} gary g.
}
} At 03:19 PM 3/23/00 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} }
} } I've heard from several SEM/TEM independent maintenance companies that
} } some of the manufacturer's have engaged in "price dumping" when
} } negotiating a service contract.
} }
} } In other words, if the manufacturer is aware that they have competition
} }
} } for a service contract they will reduce the contract by as much as forty
} }
} } percent.
} }
} } I am all for the free enterprise system, but I thought that when a
} } contract price is offered it is the same for all customers especially
} } government contracts that must abide by the GSA rules. GSA rules states
} }
} } that the price offered is the lowest price for this service. It would
} } be
} } illegal for the manufacturer (or anyone) to offer a contract at less
} } than the cost offered to GSA customers.
} }
} } I am surprised by this move as our contracts are the same as we abide
} } by
} } the GSA rules.
} }
} } Regards,
} }
} } Earl Weltmer



From daemon Fri Mar 24 08:47:27 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 23 Mar 2000 19:34:30 -0600
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK....to a GSA customer. What about to other customers? And
what precedence does one GSA contract or schedule have for
other instantiations? They are, in my experience, single point
events. They are not precedence events.

Where is the original thread that spawned this message?

Note, that in my experience, GSA contracts are distinct from other
Federal government contracts. i.e., GSA is one thing, other
gov contracts are another. GSA negotiates rates....they do not
establish them. Therefore, for a particular GSA contract or rate
structure, the rates are pre-negotiated for fed users to adopt
as per their own contracting department. Or, they can use the
GSA schedule and go with that vehicle and its added surcharge.
(Yes, GSA contracts cost more than face value). GSA has two
flavors: negotiated rates (the user does their own contracting) and
negotiated contracts (the user buys into the GSA contract and pays
a surcharge for doing so).

Which flavor are you talking about?

gg

At 09:19 PM 3/23/00 , you wrote:
} The Company providing the service does certify to the GSA customer that the
} prices given are the same or lower than the prices given to any other entity:
} public, government or private.
}
} Earl Weltmer
}
} "Dr. Gary Gaugler" wrote:
}
} } Hum.... This is very interesting. Government customers are one
} } thing, non-government customers are another. GSA rules and
} } principles do not apply to non-government customers. If you
} } would please cite the FAR that is the basis for your assertion,
} } this could help a lot to clarify your statement.
} }
} } I have never heard of "price dumping." But I have heard of
} } competition. One must keep U.S. government business separate
} } from others. Even then, how does one mingle them together?
} } On what basis is this done?
} }
} } How much of this "competition" is based on multiple unit discounts versus
} } actual price reduction? And as a consumer, what real difference
} } does it make? If the consumer can negotiate a good deal, all
} } the better for the consumer, right?
} }
} } Mixing GSA into free enterprise is like apples and oranges, so
} } to speak.
} }
} } What do you think?
} }
} } gary g.
} }
} } At 03:19 PM 3/23/00 , you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi All,
} } }
} } } I've heard from several SEM/TEM independent maintenance companies that
} } } some of the manufacturer's have engaged in "price dumping" when
} } } negotiating a service contract.
} } }
} } } In other words, if the manufacturer is aware that they have competition
} } }
} } } for a service contract they will reduce the contract by as much as forty
} } }
} } } percent.
} } }
} } } I am all for the free enterprise system, but I thought that when a
} } } contract price is offered it is the same for all customers especially
} } } government contracts that must abide by the GSA rules. GSA rules states
} } }
} } } that the price offered is the lowest price for this service. It would
} } } be
} } } illegal for the manufacturer (or anyone) to offer a contract at less
} } } than the cost offered to GSA customers.
} } }
} } } I am surprised by this move as our contracts are the same as we abide
} } } by
} } } the GSA rules.
} } }
} } } Regards,
} } }
} } } Earl Weltmer



From daemon Fri Mar 24 08:47:35 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 23 Mar 2000 22:07:51 -0600
Subject: Re: The Image Processing Tool Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On the very low end A Snappy digitizer and a surveillance
video camera produce what I would consider the minimal
acceptable image. It is good enough for some work I would
say it is comparable to a Polaroid image or slightly worse.

} From a quality per dollar point of view it can't be beat.
I would say it is good enough for collaboration, web publishing
and some publishing.

This set up and 35mm camera should meet all quality needs as
long as you don't need real-time images.
It does not compare to a top of the line dedicated system. But it does
better than anything but a dedicated system.

My best effort to date is:
http://www.couger.com/microscope/onion.jpg
This is the raw image with no enhancement and the enhanced version is:
http://www.couger.com/microscope/onionE.jpg
This version was contrast enhanced and mapped to false color.
The subject is a partially dehydrated onion cell. There are depth
of focus problems and possibly some vibration problems. There
is only 7.5 bits of information in the raw image. I attribute this
to loss of dynamic range in the camera due to age.

This was taken with a Leitz darkfield condenser and a Leitz 63x
0.85 objective with a variable aperture for darkfield work. The
camera was a monochrome RCA surveillance videocron camera
and a Snappy digitizer.

I don't think the microscope end can be improved on much in the
onion image. I still had dynamic range problems. The only solution
I can think of is to take multiple pictures at different light levels and
combine them. A high resolution CCD camera will give better
resolution but I don't think the dynamic range problem will go
away using video cameras. Maybe I will get around to
exposing a 4X5 negative and see what is really there.
But after using digital capture it is an awful lot of trouble.

I am strictly an amateur with a microscope but I do have
professional experience in digital images.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.Berkeley.EDU}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 23, 2000 1:56 PM


Try going direct:

Reindeer Games, Inc.
235 S. Main St. #201
Gainesville, FL 32601
352.384.1850

http://members.aol.com/foveapro

jcr6-at-aol.com

gg


At 06:21 PM 3/23/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking for a vendor to purchase The Image Processing Tool Kit by John Russ. I placed an order with Amazon nearly a month ago but have yet to receive anything. Hmmmm.
}
} Thank you,
}
} John B.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################



From daemon Fri Mar 24 08:47:45 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Fri, 24 Mar 2000 08:22:39 +0200
Subject: Re: The Image Processing Tool Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John

} I am looking for a vendor to purchase The Image Processing Tool Kit by
} John Russ.

I believe you can purchase it directly from John Russ.

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Fri Mar 24 08:48:07 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Fri, 24 Mar 2000 12:32:22 +0100
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott
Some aquatic invertebrates such as gastrotrichs are difficult to fix
for SEM in a life-like relaxed state because aldehyde fixatives
induce violent contraction. A paper by May+ advocates relaxing the
organisms (rotifers in this instance) first by narcosis with procaine,
or similar anaesthetics. However, in our experience gastrotrichs
can sense these anaesthetics, and considerable distortion results.
There is a considerable literature on this topic covering the
properties of EDTA, menthol, osmium tetroxide, etc. etc. for this
purpose, but mostly these solutions are ineffective at least on
gastrotrichs.

We have found sodium azide at ~0.1% to be very effective at
preventing distortion. Gastrotrichs seem not to be irritated by it, but
simply go to sleep. We have usually mounted them for LM in
glycerol, in which they can be preserved and collected for transit
or for subsequent processing and examination. The fixation/
dehydration/ HMDS procedure you suggest would probably be very
effective, but you might also try Ensikat & Barthlott's* approach of
simply examining them in the SEM in the glycerol-wet state, or
after drying them from glycerol under vacuum.

+May, L. (1985) The use of procaine hydrochloride in the
preparation of rotifer samples for counting. Verh. Internat. Verein.
Limnol. 22, 2897-2990
*Ensikat & Barthlott (1993) Liquid substitution - a versatile
procedure for sem specimen preparation of biological-materials
without drying or coating Journal of Microscopy 172, 195-203

Sodium azide is obviously a serious poison, and schoolkids cannot
handle the powder, but would it be acceptable for them to use
0.1% solution under supervision by a teacher? I guess this
depends on local legislation and attitudes, and also on the age and
level of experience and responsibility of the students.

Hope this helps
Chris

Date sent: Thu, 23 Mar 2000 16:16:36 -0600
To: Microscopy-at-sparc5.microscopy.com
} From: Scott Robinson {sjrobin-at-itg.uiuc.edu}


Dear Vladimir,

Under the circumstances you describe you are probably on the edge of the
condition where the virtual composition changes linearly with the pressure,
but this depends on the working distance and the gas that you use. However,
if you still have some pressure range to work with, then making several
measurements at a range of pressures may still allow you to use a non-linear
extrapolation.

The main problem might be that wet specimens often have a thin water film on
the sample, which may adversely affect EDS analysis. Keeping exact control
over sample temperature, pressure and humidity is required to get suitable
EDS conditions.

With best regards,

Hans Dijkstra

-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
} From: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
} Sent: Thursday, March 23, 2000 10:09 PM
} To: 'Hans Dijkstra'; Dusevich, Vladimir
} Cc: Microscopy Listserver
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Hans,
} Thank you very much for explanations.
} But for me this method will not work -
} quite often I analyze wet specimens at
} pretty high pressure (5-6 Torr).
} Thank you again,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
} } -----Original Message-----
} } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } Sent: Thursday, March 23, 2000 12:19 PM
} } To: DusevichV-at-umkc.edu
} } Cc: Microscopy Listserver
} } Subject: RE: EDS in Variable Pressure SEM
} }
} }
} } Dear Vladimir,
} }
} } The main idea of the ViP-Quant procedure is rather straight-forward and
} } elegant. It is based on the phenomenon that as the pressure increases
the
} } size of the skirt effect remains fairly constant, although the number of
} } skirt electrons increases, and thus the contribution of the skirt-effect
to
} } the EDS signal increases. This intensity increase is linear with the
pressure.
} }
} } This is valid for low- to medium pressures, or at a higher (ESEM)
pressure
} } with a very short free-path length of the skirt electrons, since in both
} } cases you can assume the skirt electrons have scattered only once. At
higher
} } pressures with longer working distances these assumptions are no longer
} } fully valid.
} }
} } So to do an EDS analysis you take 2 measurements at different pressures,
the
} } low-pressure one at a pressure where you can just avoid charging, and a
} } high-pressure one at at least twice that pressure. The measured
intensities
} } of all elements are then plotted as a function of pressure, and
extrapolated
} } to pressure zero, i.e. high vacuum. The extrapolated results will be
very
} } close to the results hat would have been obtained directly if the sample
} } could have been analyzed at high vacuum conditions.
} }
} } Of course anyone can do this plotting and extrapolation manually, that
is:
} } if your EDS software allows you to enter intensities manually! The only
} } thing the EDAX ViP-Quant is offering as an extra is that the software
does
} } it automatically for you. There are no proprietary miracles involved,
EDAX
} } has just listened carefully to what science has to offer....
} }
} } Best regards,
} }
} } Hans Dijkstra
} }
} } Disclaimer: The above is my personal opinion, and not necessarily
EDAX's.
} } -------------------------------------------------------------
} } EDAX Europe www.edax.com
} } Ringbaan Noord 103 Tel.: +31-13-5364000
} } P.O. Box 4144 Fax.: +31-13-5356279
} } 5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
} } the Netherlands
} } -------------------------------------------------------------
} }
} } } -----Original Message-----
} } }
} } } From: "Dusevich, Vladimir", INTERNET:DusevichV-at-umkc.edu
} } } To: Everett Ramer, INTERNET:Everett.Ramer-at-netl.doe.gov
} } } "'Hans Dijkstra'", INTERNET:hans.dijkstra-at-edax.nl
} } }
} } } CC: Joergen Bilde-Soerensen 5802, INTERNET:j.bilde-at-risoe.dk
} } } [unknown], INTERNET:Microscopy-at-sparc5.microscopy.com
} } }
} } } Date: 3/22/00 4:35 PM
} } }
} } } RE: RE: EDS in Variable Pressure SEM
} } }
} } }
} } }
} } --------------------------------------------------------------
} } ----------
} } } The Microscopy ListServer -- Sponsor: The Microscopy
} } Society of America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } --------------------------------------------------------------
} } ---------.
} } }
} } }
} } } Hans,
} } } Could you please explain the main idea of the
} } } Vip-Quant algorithm. I cannot even imagine
} } } how quantitative analysis could have nearly the
} } } same accuracy as in high-vacuum mode without
} } } a priori knowledge of the composition of
} } } surrounding areas.
} } } Thank you,
} } }
} } } Vladimir
} } }
} } }
} } } Vladimir M. Dusevich, Ph.D.
} } } Electron Microscope Lab Manager
} } } 3127 School of Dentistry
} } } 650 E. 25th Street
} } } Kansas City, MO 64108-2784
} } }
} } } Phone: (816) 235-2072
} } } Fax: (816) 235-5524
} } } Web: http://www.umkc.edu/dentistry/microscopy
} } }
} } } } -----Original Message-----
} } } } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } } } Sent: Tuesday, March 21, 2000 7:54 AM
} } } } To: Everett Ramer
} } } } Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} } } } Subject: RE: EDS in Variable Pressure SEM
} } } }
} } } }
} } } } Dear Everett,
} } } }
} } } } The extrapolation method (aka Variable Pressure Method)described by
Dr.
} } } } Bilde-Sorenson has been implemented by EDAX in a software feature
called
} } } } ViP-Quant. With this method the EDS analysis under Bad-Vacuum
conditions can
} } } } be done with nearly the same accuracy as under High-Vacuum
conditions.
} } } } Although this method was developed with the ESEM microscope in mind,
it of
} } } } course can be applied to all Bad-Vacuum scanning electron
microscopes.
} } } }
} } } } Please contact your local EDAX representative for more information
and a
} } } } copy of the new ViP-Quant brochure, or request one through
www.edax.com.
} } } }
} } } } With best regards,
} } } }
} } } } Hans Dijkstra



From daemon Fri Mar 24 08:48:26 2000



From: earlw-at-pacbell.net
Date: Fri, 24 Mar 2000 06:38:04 -0800
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Allen,

Thanks for the info. I really didn't want to go through & look up the FAR
again.

The GSA wasn't my real inquiry anyway. I just wanted to find out how widespread
this practice extends. The GSA issue seems to get in the way.

OK to rephase: How many have experienced an extreme reduction in service
contract price (20% or more) when confronted with competition?

Thank You,

Earl Weltmer


"Allen R. Sampson" wrote:

} Actually, both are right here. Government stipulations have apparently
} eased recently, but a part of the government contract procedure has in the
} past been a certification by the service provider that the rates being
} quoted are normal and appropriate. This has essentially required details
} of a few other comparable contracts that were similar in price and
} requirements.
}
} I have not had to go through that particular body cavity search for some
} time. I assume the government learned that certifications of this sort are
} basically worthless, as there are companies large and small who are willing
} to do a little spin control in order to secure contracts. Government
} agencies are probably unlikely to bother verifying compliance with their
} regulations - not a problem in this field alone.
}
} My memory goes only so far, as does my desire to look up old records.
} However, if you're really interested, you may want to start with the old
} DAR (Defense Acquisition Regulation) 7-1903.41 - Service Contract Act of
} 1965, also updated and known as FAR 52.222-40. In the late 80's, it seems
} to have been a part of the small business set-aside program (FAR 52.219-04)
} to require a price list or a verifiable listing of 3 similar items sold to
} establish a market price. You might also look into the Walsh-Healey Public
} Contract Act, FAR 52.222-20.
}
} DOD FAR Supplement (46 CFR Chapter 2) clause 11.111-10 states - "...The
} Contractor agrees that the prices for the supplies or services furnished
} under this contract are as low or lower than those charged the supplier's
} most favored customer for comparable quantities under similar terms and
} conditions, in addition to any discounts for prompt payment."
}
} Frankly each government contract I have generates about 1/2" of paper work
} per year. I give little concern to pricing matters, either I win a
} contract or not. The same goes for commercial customers. I try to price
} my services to all customers based on the expenses I expect to incur and a
} modest income for myself (perhaps way too modest, I reflect, as the time to
} finish the tax year has come). There are many other regulations and laws
} that I have to pay more attention to.
}
} Whether these, and other, regulations, laws and requirements have any
} actual practical effect is open to debate. I can't say that I have noticed
} any recent trend for manufacturers to discount their service contracts -
} although I don't normally inquire about other bids submitted. I wouldn't
} be surprised, though, if manufacturers are finding it advantageous to
} discount service prices now to capture customers while the capital
} expenditures are running high in the current economy. However, when those
} capital budgets dry out in the next economic cycle, they will find that the
} service end will have a larger effect on their profitability and they will
} increase their profit margins as best they can.
}
} Consider this a normal result of the economic cycles that we are all slaves
} to. As a third party service provider, you can generally think of me as an
} independent car repair shop. In good times, people will go to
} manufacturer's service, or buy a new car - in bad times they will make
} every effort to stretch their capital investment. In good economic times,
} I catch a good deal of lower end business from those who have instruments
} orphaned by their manufacturer or are very price conscious. In bad
} economic times, I'm the guy who can help you avoid laying off personnel by
} reducing your costs.
}
} In either case, just be glad that there are independent service sources
} available for these instruments and consider that they will only be there
} as long as it is economically feasible for them to survive all economic
} scenarios. That is even more true for manufacturers who would rather sell
} you a new instrument than service a 3 year old instrument. That
} short-sighted corporate position has proven to be a death knell in this
} industry. In many cases it has only been the availability of third party
} service that has allowed a company to survive in a given market for a
} little longer. Public relations is a two-sided blade - it can cut real
} deep when times are bad.
}
} Government laws, rules and regulations can have a significant effect on
} manufacturers serving both government and commercial customers. As has
} been stated before, manufacturers wishing to do business with the
} government are often required to provide service for a stated period of
} time and, as stated here, are required to provide service at a market rate.
} A first year law student would recognize that, in this country, commercial
} concerns benefit from the standards set by the government. It would
} behoove any company to provide an even pricing structure across the board,
} as any other position opens them up to legal action from either side.
}
} Since the government rules require comparison under similar "terms and
} conditions", the question of multiple instrument discounts is appropriately
} accounted for. We are not talking '"apples and oranges" here. Rather, we
} are talking about a closed feedback loop where any change in either side
} must affect the other. The only wiggle-room here is in the quality of
} service provided. Yes, the consumers, both government and commercial, can
} bid the service price down. But in the end, only the service quality will
} be compromised.
}
} Allen R. Sampson, Owner
} Advanced Research Systems, St. Charles, Illinois 60174
} voice 630.513.7093 fax 630.513.7092
}
} -----Original Message-----
} From: Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
} Sent: Thursday, March 23, 2000 6:18 PM
} To: Earl Weltmer
} Cc: MSA listserver
} Subject: Re: Dumping of Service Contracts costs
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hum.... This is very interesting. Government customers are one
} thing, non-government customers are another. GSA rules and
} principles do not apply to non-government customers. If you
} would please cite the FAR that is the basis for your assertion,
} this could help a lot to clarify your statement.
}
} I have never heard of "price dumping." But I have heard of
} competition. One must keep U.S. government business separate
} from others. Even then, how does one mingle them together?
} On what basis is this done?
}
} How much of this "competition" is based on multiple unit discounts versus
} actual price reduction? And as a consumer, what real difference
} does it make? If the consumer can negotiate a good deal, all
} the better for the consumer, right?
}
} Mixing GSA into free enterprise is like apples and oranges, so
} to speak.
}
} What do you think?
}
} gary g.
}
} At 03:19 PM 3/23/00 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} }
} } I've heard from several SEM/TEM independent maintenance companies that
} } some of the manufacturer's have engaged in "price dumping" when
} } negotiating a service contract.
} }
} } In other words, if the manufacturer is aware that they have competition
} }
} } for a service contract they will reduce the contract by as much as forty
} }
} } percent.
} }
} } I am all for the free enterprise system, but I thought that when a
} } contract price is offered it is the same for all customers especially
} } government contracts that must abide by the GSA rules. GSA rules states
} }
} } that the price offered is the lowest price for this service. It would
} } be
} } illegal for the manufacturer (or anyone) to offer a contract at less
} } than the cost offered to GSA customers.
} }
} } I am surprised by this move as our contracts are the same as we abide
} } by
} } the GSA rules.
} }
} } Regards,
} }
} } Earl Weltmer



From daemon Fri Mar 24 09:01:15 2000



From: Kingsley Micklem :      kingsley.micklem-at-cellular-science.oxford.ac.uk
Date: Fri, 24 Mar 2000 08:50:13 -0600
Subject: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We find that we get better silver staining of cross-sections of hair
viewed bt TEM when embedded in LR White than Araldite resin.

We are looking for a way to allow us to orientate a bundle of hair fibres
(about 5mm long) and keep the bundle intact whilst heat-curing the LR
White anaerobically. We do not have the means to lower the temperature of
the resin, nor use UV light for curing.

Can anyone suggest a means of keeping the bundle of hairs intact within
the LR White whilst embedding and curing? Any support must be able to
withstand the effects of acetone and LR White resin in liquid form.

Thanks for your attention

Jeremy Sanderson
Please reply to jb_sanderson-at-yahoo.com

**********************************************************************
Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
Director, Medical Informatics Unit * CELLULAR SCIENCE
University Department of Cellular Science *
Room 5501, John Radcliffe Hospital, * OXFORD
Headington, Oxford, OX3 9DU *
Tel 44 (1865) 222039 * website:
FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
**********************************************************************




From daemon Fri Mar 24 09:20:32 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 24 Mar 2000 10:18:53 -0500
Subject: TEM, Biological, sinking of tissue in sucrose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is a question for those of you who use sucrose to provide
cryoprotection for tissues:

We are preparing perfusion fixed brain tissue by freeze substitution for
post-embedding immuno gold. Following fixation we prepare 500 micron
vibratome sections, which we then wish to infiltrate with 2 M sucrose in
buffer prior to freezing. We have been transferring the brain sections to
1 M sucrose, then when they sink, transferring again to 2 M. The problem
is that the sections never sink in the 2 M sucrose.

Do others have this problem? Do we have to cut the sections into small
pieces first, or is there some other way to get them to go under? Or
should we not worry about making them sink?

Thanks for your advice.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936




From daemon Fri Mar 24 09:31:30 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 24 Mar 2000 09:28:22 -0600
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have done similar things in a manner such as this:
Take the cap off a BEEM capsule and lay it down open side up. Lay
your hair fibers flat in the cap and add LRW. Place a glass slide on
top of the cap to seal it off during polymerization. Heat
polymerize. Use a dremel moto-tool or jewelers hacksaw to trim out a
rectangle of the LRW. Good luck, Tom




}
}
} We find that we get better silver staining of cross-sections of hair
} viewed bt TEM when embedded in LR White than Araldite resin.
}
} We are looking for a way to allow us to orientate a bundle of hair fibres
} (about 5mm long) and keep the bundle intact whilst heat-curing the LR
} White anaerobically. We do not have the means to lower the temperature of
} the resin, nor use UV light for curing.
}
} Can anyone suggest a means of keeping the bundle of hairs intact within
} the LR White whilst embedding and curing? Any support must be able to
} withstand the effects of acetone and LR White resin in liquid form.
}
} Thanks for your attention
}
} Jeremy Sanderson
} Please reply to jb_sanderson-at-yahoo.com
}
} **********************************************************************
} Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
} Director, Medical Informatics Unit * CELLULAR SCIENCE
} University Department of Cellular Science *
} Room 5501, John Radcliffe Hospital, * OXFORD
} Headington, Oxford, OX3 9DU *
} Tel 44 (1865) 222039 * website:
} FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
} **********************************************************************

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Mar 25 08:58:05 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 24 Mar 2000 09:31:16 -0600
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeremy,
We have had good luck keeping roots straight by sanwiching
them between films of formvar on wire loops. We make a loop with a
3mm diameter and a long stem out of very thin copper wire (36 gauge
in usa). We then cast rectangles of formvar about 4 mm x 8 mm and
line the loop up over the floating rectangle so that the plane of the
loop is parallel to the short side of the rectangle and right in the
middle. Then we quickly plunge the loop straight down onto the
formvar and into the water and pull it back out again. This gives us
a nice film on the loop. Give this at least a few hours to dry (but
they will keep for ages--we plant the stem of the loop in some wax
and cover with a beaker). Then when ready to go, place your sample,
either before or after fixation, on the loop and repeat with a second
formvar rectangle.Now you have the sample sandwiched. It is very nice
for small samples because solution exchange becomes a snap. The
formvar definitely stands up to actetone, butyl methyl methacrylate,
Spurs, and I am 95% sure LR white. It is easy to dehydrate through
and get other things through even antibodies go through (although
they are slowed down a bit).

Hope this helps.

You got more questions, let me know.

Tobias Baskin


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Sat Mar 25 08:58:06 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 24 Mar 2000 09:39:19 -0600
Subject: RE: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are facing a similar problem here and have just had some preliminary
success with polymerizing LR White in a vacuum dessicator placed in an oven.
We were using gelatin capsules full of LR White placed upside down over
coverslips upon which cells have been grown. Standard polymerization did
not adhere the gel caps to the coverslips, supposedly due the presence of
oxygen (it was worth a try). However, the trial run in the dessicator seems
to have worked well, and the cover slip detached cleanly when placed in
liquid nitrogen.

You might try this with flat embedding molds to preserve the orientation of
your bundles. If you do, please let us know the results.

Another approach might be to embed the hair bundles and later cut them out
of the LR White in a piece of resin, orient them the way you want, and glue
the resin piece onto the tip of a blank block for ultramicrotomy.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We find that we get better silver staining of cross-sections of hair
viewed bt TEM when embedded in LR White than Araldite resin.

We are looking for a way to allow us to orientate a bundle of hair fibres
(about 5mm long) and keep the bundle intact whilst heat-curing the LR
White anaerobically. We do not have the means to lower the temperature of
the resin, nor use UV light for curing.

Can anyone suggest a means of keeping the bundle of hairs intact within
the LR White whilst embedding and curing? Any support must be able to
withstand the effects of acetone and LR White resin in liquid form.

Thanks for your attention

Jeremy Sanderson
Please reply to jb_sanderson-at-yahoo.com

**********************************************************************
Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
Director, Medical Informatics Unit * CELLULAR SCIENCE
University Department of Cellular Science *
Room 5501, John Radcliffe Hospital, * OXFORD
Headington, Oxford, OX3 9DU *
Tel 44 (1865) 222039 * website:
FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
**********************************************************************




From daemon Sat Mar 25 08:58:08 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Fri, 24 Mar 2000 18:07:16 +0100
Subject: TEM: Personal TEM website, updated.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


off the top of my head:
pack hairs into a small tube made out of thin paper - velin tissue or
Rizla cigarette paper wrapped round a cocktail stick - the gum on
the edge of a cigarette paper should survive the resin


Date sent: Fri, 24 Mar 2000 08:50:13 -0600
To: Microscopy-at-sparc5.microscopy.com
} From: Kingsley Micklem {kingsley.micklem-at-cellular-science.oxford.ac.uk}


Hi, all,

Many suggestions have been given to me after my posting the Personal TEM Website http://syli.homepage.com in this list sever. Thanks a lot to these contributers!

Now the site has been updated according to these good suggestions. More journals are added into the TEM-related Journals. and an additional page for JOB LIST and RESUMES was included for head-huntings in TEM region. Now you may send me your head-hunting ads or your resume to me and I will place it in this part. Thanks again.

Shu-You Li
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Please visit my new homepage - a personal website on transmission electron microsocpy (TEM) - at http://syli.homepage.com at your convenience!
It contains not only my current research work, but also LINKS to useful journal informations, instruction for authors, on-line EELS database, periodic table, physical constants, online TEM course and etc. It also contains JOB list and RESUMEs for head-hunting in TEM region.
I am looking forward to your invaluable suggestions!
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************



From daemon Sat Mar 25 08:58:09 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Fri, 24 Mar 2000 12:14:29 -0500 (EST)
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK - this is extremely low-tech, but it works (if none of the other
suggestions you receive do the trick). Cotton thread! I've made bundles of
fibres by tying them up with fine sewing thread. You can tie several
places along the length of the bundle so it will be held more firmly if
you need to. You can process, dehydrate, and embed. Just make certain that
you have cotton thread - some of the polymer ones might melt in the
solvents or resins.

I've also heard of using fishing monofilament for stuff that doesn't
require nasty solvents - this is probably just the "guy version" of the
thread technique :)

Tamara Howard
CSHL



On Fri, 24 Mar 2000, Kingsley Micklem wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We find that we get better silver staining of cross-sections of hair
} viewed bt TEM when embedded in LR White than Araldite resin.
}
} We are looking for a way to allow us to orientate a bundle of hair fibres
} (about 5mm long) and keep the bundle intact whilst heat-curing the LR
} White anaerobically. We do not have the means to lower the temperature of
} the resin, nor use UV light for curing.
}
} Can anyone suggest a means of keeping the bundle of hairs intact within
} the LR White whilst embedding and curing? Any support must be able to
} withstand the effects of acetone and LR White resin in liquid form.
}
} Thanks for your attention
}
} Jeremy Sanderson
} Please reply to jb_sanderson-at-yahoo.com
}
} **********************************************************************
} Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
} Director, Medical Informatics Unit * CELLULAR SCIENCE
} University Department of Cellular Science *
} Room 5501, John Radcliffe Hospital, * OXFORD
} Headington, Oxford, OX3 9DU *
} Tel 44 (1865) 222039 * website:
} FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
} **********************************************************************
}
}
}
}



From daemon Sat Mar 25 08:58:12 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Fri, 24 Mar 2000 13:09:13 -0500
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Of course you should all consider that cutting or grinding ANY embedding
resin should be undertaken with the knowledge that resin dust can cause
serious health effects. I don't know if any of my allergies or other health
quirks have been 'encouraged' from exposure to resin dust or solvents, but
judging from the discussions on the listserver about some people's
experiences with resins, I recommend that you treat that dust with the same
respect as paraformaldehyde powder or asbestos!

Just thought I'd share that safety concern.

Gregg Sobocinski
Parke Davis Pharmaceutical Research
Ann Arbor, Michigan, USA
Gregg.Sobocinski-at-wl.com


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, March 24, 2000 10:28 AM
To: Kingsley Micklem
Cc: Microscopy-at-sparc5.microscopy.com


I have done similar things in a manner such as this:
Take the cap off a BEEM capsule and lay it down open side up. Lay
your hair fibers flat in the cap and add LRW. Place a glass slide on
top of the cap to seal it off during polymerization. Heat
polymerize. Use a dremel moto-tool or jewelers hacksaw to trim out a
rectangle of the LRW. Good luck, Tom




}
}
} We find that we get better silver staining of cross-sections of hair
} viewed bt TEM when embedded in LR White than Araldite resin.
}
} We are looking for a way to allow us to orientate a bundle of hair fibres
} (about 5mm long) and keep the bundle intact whilst heat-curing the LR
} White anaerobically. We do not have the means to lower the temperature of
} the resin, nor use UV light for curing.
}
} Can anyone suggest a means of keeping the bundle of hairs intact within
} the LR White whilst embedding and curing? Any support must be able to
} withstand the effects of acetone and LR White resin in liquid form.
}
} Thanks for your attention
}
} Jeremy Sanderson
} Please reply to jb_sanderson-at-yahoo.com
}
} **********************************************************************
} Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
} Director, Medical Informatics Unit * CELLULAR SCIENCE
} University Department of Cellular Science *
} Room 5501, John Radcliffe Hospital, * OXFORD
} Headington, Oxford, OX3 9DU *
} Tel 44 (1865) 222039 * website:
} FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
} **********************************************************************

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Mar 25 08:58:14 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 24 Mar 2000 13:39:17 -0500
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi earl-
i've been considering the whole service contract routine lately since i
have an older sem, a newer fesem, and a vanilla tem under contract. the
sum of my contracts (from the vendors) is about $40K. i have a hard time
justifying this level of expenditure, but have been burned in the past with
replacement part costs under "limited" arrangements. in my role i have to
recover all lab costs, and contracts are a substantial portion of them.

my main vendor (SEMs) has offered me a 5% "discount". this seems a bit
light (to me) because i am limited in terms of my cost recovery mechanisms.
a commercial or industrial lab would seem to have more flexibility than a
shared facility at a small university. currently i'm negotiating with them
to extend this discount. i'm not sure what will happen if i can't keep
these costs under control...perhaps this is an opportunity for a crafty
entrepreneur. at the very least it may be a signal to look for 3-rd party
service, in-house methods, and/or go bare on older equipment.

brian
********************
} OK to rephase: How many have experienced an extreme reduction in service
} contract price (20% or more) when confronted with competition?
}
} Thank You,
}
} Earl Weltmer

----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Sat Mar 25 08:58:20 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 24 Mar 2000 15:22:24 -0500
Subject: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have for sale, or know of a supplier of Au/Pd target for the
older model Balzers-Union Sputtering Device Type 07120-A, serial number
235? It is an early version of that coater series and uses targets for an
SCD010 type coater with serial numbers 101-317. The target is ~5 cm
diameter with a threaded mounting hole. Thanks!


*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Sat Mar 25 08:58:22 2000



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Fri, 24 Mar 2000 13:07:55 -0800
Subject: EPMA Standards and QA, Yucca Mountain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,
We are trying to do some electron microprobe work on the Yucca Mountain
Project for the DOE. However, we may need external verification of our
standards by a laboratory that is on the Qualified Supplier List,
specifically for the YMP. Therefore, unless a lab has done work
directly for Yucca Mountain specifically, they don't count (even DOE run

national labs). This is all according to our QA people, and I am hoping

that someone out there may have direct knowledge of the YMP QA and/or
know someone who does. Please respond offline to me. Many thanks in
advance,
Sarah

************************************************************************

Sarah A.W. Lundberg Lab
(EPMA) (702) 895-2660 or
Electron Microanalysis and
(SEM) (702) 895-2462
Imaging Laboratory
Office (702) 895-1134
Department of Geoscience, UNLV
Fax (702) 895-4064
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010 Dept
Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
4489 De Forest St.
Las Vegas, NV 89103
(702) 871-9635
************************************************************************





From daemon Sat Mar 25 08:58:32 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Fri, 24 Mar 2000 18:56:07 -0500
Subject: Laser for laser tweezer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am thinking of trying to put together a laser tweezer setup on my
microscope. It doesn't seem too difficult for a simple system, but I
am having trouble finding the appropriate laser so I don't have to
build it myself from the module. Has anyone out there found a good
source for a near IR laser appropriate for this application? Any
other words of wisdom for a laser/laser tweezer novice appreciated.
Dave Knecht
--

************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Sat Mar 25 08:58:35 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Fri, 24 Mar 2000 16:36:36 -0800
Subject: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Micklem,
I embed a variety of fibers for cross-sectioning using the following.
Collect little round pieces of paper from your office paper hole punch.
Using a sharp needle punch an appropriately sized hole in the center of
several of them. Thread your fibers to be embedded through the center hole
of the paper circles. The number required will depend on the length,
diameter and mass of the fibers to be sectioned. I find that a fine pair
of tweezers and a disectin microscope make this job easier. Gently place
this assembly into your embedding capsule (I use BEEM 00). With a pipette,
slowly add resin down the sides to fill the capsule (I use Spurrs with
Z-6040 adhesion promoter). The fiber/paper assembly will self-center in
the capsule and maintain this orientation through curing. Be careful with
your choice of paper, it must be dry, and some porous paper will outgas
bubbles. Test paper samples ahead of time to identify a suitable type.
I'm sure you can adapt this to work with LR White.

Good luck,


Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Kingsley Micklem
[SMTP:kingsley.micklem-at-cellular-science.oxford.ac.uk]
Sent: Friday, March 24, 2000 6:50 AM
To: Microscopy-at-sparc5.microscopy.com


We find that we get better silver staining of cross-sections of hair
viewed bt TEM when embedded in LR White than Araldite resin.

We are looking for a way to allow us to orientate a bundle of hair fibres
(about 5mm long) and keep the bundle intact whilst heat-curing the LR
White anaerobically. We do not have the means to lower the temperature of
the resin, nor use UV light for curing.

Can anyone suggest a means of keeping the bundle of hairs intact within
the LR White whilst embedding and curing? Any support must be able to
withstand the effects of acetone and LR White resin in liquid form.

Thanks for your attention

Jeremy Sanderson
Please reply to jb_sanderson-at-yahoo.com

**********************************************************************
Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
Director, Medical Informatics Unit * CELLULAR SCIENCE
University Department of Cellular Science *
Room 5501, John Radcliffe Hospital, * OXFORD
Headington, Oxford, OX3 9DU *
Tel 44 (1865) 222039 * website:
FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
**********************************************************************






From daemon Sat Mar 25 08:58:45 2000



From: Pbgrover-at-aol.com
Date: Fri, 24 Mar 2000 22:53:45 EST
Subject: SEM: bacteria on cotton thread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Esteemed Colleagues,

A client asked me to photograph bacilli and endospores on a cotton thread (he
supplied the sample already sputtered with au/pd). I took one really nice
photo, then began experiencing movement of the thread fibers due to
electrostatic charges. It seems the cells & endospores are concentrated at
the unsupported ends of the fibers; when I look at thte interior, bundled
fibers, which are better restrained, they are practically devoid of cells. I
can only guess that the cells/spores were drawn to the outer edges of the
thread as the buffer evaporated.

Any advice, insights or ideas will be rewarded with a big Thank You, Thank
You, Thank You.

Cheers,

Paul Grover :o)
Chief Microscopist and Bottle Washer
Microvista Laboratory
1220 Cincinnati St.
Lafayette, IN 47904




From daemon Sat Mar 25 08:58:51 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Fri, 24 Mar 2000 23:56:32 -0600
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Sobocinski, Gregg" {Gregg.Sobocinski-at-WL.com}
}
}
} Of course you should all consider that cutting or grinding ANY embedding
} resin should be undertaken with the knowledge that resin dust can cause
} serious health effects. I don't know if any of my allergies or other
health
} quirks have been 'encouraged' from exposure to resin dust or solvents, but
} judging from the discussions on the listserver about some people's
} experiences with resins, I recommend that you treat that dust with the
same
} respect as paraformaldehyde powder or asbestos!
}
} Just thought I'd share that safety concern.

The unrecalled products cause more reaction problems than the
cured product.

But as one that has been too careless with air quality don't risk
you health breathing dusts, chemicals or solvents any more than
absolutely necessary.

I think the reaction products of an automobile my be worse than
most things in the lab so you may need to wear you respirator on
your drive to work. About the time the catalytic converter came
along my asthma really flared. some how I don't thing H2SO3 is
good for me.

Take good care of you health if the average age at death continues
it present course you may have to live to 120 or more.

Take care
Gordon

G. C,. Couger
624 Cheyenne
Stillwater, OK 74075-1411
405 624 2855 between 3:00 pm to 1:00 am CST
405 742 2758 cell




From daemon Sat Mar 25 08:59:04 2000



From: greg erdos :      gwe-at-biotech.ufl.edu
Date: Sat, 25 Mar 2000 07:52:40 -0800
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We solved the resin dust/ Dremel tool problem by rigging a small vacuum
cleaner with the intake very close to where we would be grinding, which was
usually under a disecting scope. I got the idea while watching a cast
being cut from a broken foot. The cast cutter had a vacuum attached.
Our rig takes care of the fine particles, while larger ones generally fall
to the earth and can be cleaned up later.
This more of an issue with epoxies, I suspect. LR White is a dental resin,
I believe, and the dentist will grind in your mouth or over you using your
chest as a table. I don't know if that means it is safe.

Greg Erdos
University of Florida
Greg Erdos
5410 SE 185th AVe
Micanopy, FL 32667
352-466-0843



From daemon Sat Mar 25 08:59:05 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sat, 25 Mar 2000 12:57:13 +0000
Subject: Re: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might be able to buy a metal foil of a suitable size and fix it to
what you already have (or a custom made holder) using silver-loaded
epoxy?

Goodfellow Metals (England) used to have such foils. I could check
this further if you wish, plus send you their contact details when I
am back in the lab.

Regards - Keith


_______________________________
Dr. Keith Ryan
Marine Biological Association of the UK
The Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. ++44 (0)1752 633249
Tel. ++44 (0)1752 633279
The 279 number has an answering machine

Fax ++44 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Sun Mar 26 11:26:58 2000



From: RamaSubrahmanyam K :      ramu8sp-at-ccs.iitb.ernet.in
Date: Sat, 25 Mar 2000 14:01:44 -0600
Subject: Required help to etchquartz

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I had done an evapouration of cobalt on a quartz sample. Now I want to
etch the quartz sample to remove the substrate so that I can do
Transmission Microscopy for charactrization. My problem is that If I etch
the substrate with HF will it be harmful to carbon? If it is so what are
the other materials that can be used to etch quartz.
I will be very pleased if you can answer me.
Thanking you
Ramu,
Senior student in M.Sc,
Dept of Physics,
IIT Bombay.

Adress:
Rama Subrahmanyam .K
H:7, R:227,
IITB, Powai,
Mumbai- 400076.
ph:5781049.

meet me at ramu8sp-at-ccs.iitb.ernet.in
suvee-at-wowmail.com,
srisuvee-at-yahoomail.com
*******************************************************************************




From daemon Sun Mar 26 11:27:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 25 Mar 2000 19:05:16 -0600
Subject: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of some sort of vacuum vessel that
would hold a good number of boxed specimens under
vacuum? I have several mechanical pumps (dual stage)
but have not seen any sizable, sturdy containers. I would
like to store my specimens under a vacuum at all times
unless loading into the SEM. The purpose is to keep any
fungi, moisture and dust from contaminating the specimens.

Vendor responses are welcome.

gary g.



From daemon Sun Mar 26 11:27:27 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 26 Mar 2000 21:53:47 +1000
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The problem with large vacuum containers is strength and therefore cost. Its
those 14.7 pounds for every sq inch the atmosphere exerts on vacuum vessels, a
fact EMists know and understand. Some don't realize that another order of
magnitude in high vacuum makes no real difference to that pressure and a very
poor vacuum still exerts over 14 lbs. on every sq inch of surface area.

The practical and economic solution is the use airtight cabinets with drying
agents or a trickle of dry nitrogen gas. Afterall its mostly moisture that
impedes pumping in the SEM or causes specimens and coatings to fall apart with
time.
Disclaimer: Proscitech ships world-wide a large of desiccating cabinets and
vacuum desiccators. See Online } Contents } Page E7
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Sunday, March 26, 2000 11:05 AM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:}
} Does anyone know of some sort of vacuum vessel that
} would hold a good number of boxed specimens under
} vacuum? I have several mechanical pumps (dual stage)
} but have not seen any sizable, sturdy containers. I would
} like to store my specimens under a vacuum at all times
} unless loading into the SEM. The purpose is to keep any
} fungi, moisture and dust from contaminating the specimens.
}
} Vendor responses are welcome.
}
} gary g.
}



From daemon Sun Mar 26 11:27:27 2000



From: Ric Felten :      smartech-at-javanet.com
Date: Sun, 26 Mar 2000 08:42:36 -0500
Subject: SEM, Does Any Know the Expected Resolution of a "JEOL, JSM 6400" at High kV

Contents Retrieved from Microscopy Listserver Archives
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I am trying to help a new SEM user w/ a tough resolution shot. For some
reason he was not able to find the quoted resolution for the SEM (JEOL JSM
6400) he was using. If anybody out there happens to know its exact or
approximate quoted resolution, that would be very helpful. His electron
source is W.

Thanks

Ric



From daemon Sun Mar 26 16:23:18 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 26 Mar 2000 09:58:37 -0600
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


thanks to the many responders about this post. Let me add some
more info to help zero in on a solution--if there is one.

The reason I am seeking a vacuum container is two-fold. First,
I have very limited N2 availability. I use industrial grade bottled N2
and dry it with a molecular sieve for venting my SEM chamber
during specimen exchange. I vent at about 5psi. The bottles
are at 1900 psi and hold 130cu ft of N2. One bottle lasts about
two months. I have two bottles--on-line and standby. A "trickle"
of N2 through a dessicator would work but for how long per
bottle? I don't know.

The other factor for a vacuum container is that if a specimen is
under a roughing pump vacuum, if it is at all defective, the body
of the specimen will implode. Thus saving the time of fooling with
it in the SEM. If it does not implode, then it will be dry and not
require very much pump down time before opening the column
isolation valve. Thus ensuring minimal effect on the column ion
pump.

Steve D'Angelo showed a very nice metal dessicator unit, which
would be great if I had a lot of N2....I presume a lot of N2. If
I take one of my 130 cu ft cylinders and leak N2 into the dessicator,
about how long will it last? And what is the minimum leak pressure
needed to make the dessicator function as a drying unit? I could
get another cylinder of N2 and another sieve for this unit if the
gas volume was sufficient to run the box for two to three months.

Appreciate your feedback.

gary g.




From daemon Mon Mar 27 07:34:21 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Sun, 26 Mar 2000 23:25:09 -0500
Subject: position needed immediately - slight revision

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ADVERTISEMENT

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characterization or related areas. The person will be responsible for
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maintenance, equipment installation, operation and maintenance, and be an
investigator in contracted research. The person must be able to conduct
independent research and development in materials characterization with an
emphasis in ion beam technology and have the ability to interact with
students and provide instruction in the use and interpretation of data.
The individual must have extensive knowledge and experience in the
operation and maintenance of materials characterization equipment. AMPAC
is particularly interested in individuals desiring an academic setting to
further their professional goals. The applications will be reviewed
beginning April 17, 2000 and will continue to be reviewed until the
position is filled.

Applicants should send vitae and three references to Dr. Vimal Desai,
Director, 12424 Research Parkway, Suite 408, Orlando, FL 32826. UCF is an
equal opportunity/affirmative action employer. As an agency of the State
of Florida, all application materials and selection procedures are
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*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Mon Mar 27 07:34:21 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sun, 26 Mar 2000 21:09:51 -0800 (PST)
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,
Are you concerned about rmp backstreaming, thereby contaminating your
specimens with hydrocarbon. I assume you are using a filter with a baking
element?
-Ken




From daemon Mon Mar 27 07:34:41 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 27 Mar 2000 08:24:19 +0100 (BST)
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,
How about a TEM film dessicator? Available from most EM suppliers,
vacuum companies and some scrapped TEMs.

Ron

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Mon Mar 27 07:34:47 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Mon, 27 Mar 2000 10:35:33 +0100
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg

you mention using a small vacuum cleaner to clean up resin dust. You
don't specify whether it has an exhaust filter so I think I should just
mention that the typical domestic vacuum cleaner exhausts fine
particulates and may well make the problem worse because it may be
pumping the very size of resin particles out that you want to avoid. We
once used a small portable vacuum cleaner for cleaning up resin dust but
stopped when I realized that there was a potential risk.

I know that there are now domestic and industrial vacuum cleaners which
have very fine exhaust filters and there are also the small
photocopier/toner vacuum cleaners. Has anyone investigated their use for
resin dust?

Malcolm

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

greg erdos wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We solved the resin dust/ Dremel tool problem by rigging a small vacuum
} cleaner with the intake very close to where we would be grinding, which was
} usually under a disecting scope. I got the idea while watching a cast
} being cut from a broken foot. The cast cutter had a vacuum attached.
} Our rig takes care of the fine particles, while larger ones generally fall
} to the earth and can be cleaned up later.
} This more of an issue with epoxies, I suspect. LR White is a dental resin,
} I believe, and the dentist will grind in your mouth or over you using your
} chest as a table. I don't know if that means it is safe.
}
} Greg Erdos
} University of Florida
} Greg Erdos
} 5410 SE 185th AVe
} Micanopy, FL 32667
} 352-466-0843



From daemon Mon Mar 27 07:34:49 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 27 Mar 2000 21:16:37 +1000
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In Australia we can readily purchase industrial dry N2, this obviates the
drying agent and filtering of the gas. In WDS, gas proportional spectrometers
are fed with about one bubble a second (holding the outlet into water). At that
rate a cylinder routinely last for over a year and although an expensive gas is
used for that purpose, cylinder rental invariably is the greater cost.
A tight drying cabinet only requires a similar amount of gas to maintain a
positive pressure, however slight. Specimens added to the cabinet should be dry
and I think that a tray of desiccant is a good idea.

No additional cylinder would be required since Gary already has two. I used to
deplete the N2 cylinder after use on the EMs, to nitrogen burst during film
development. Since the EM duty cylinder does not go empty unexpectedly, the
cylinder feeding the drying cabinet can be removed in time obtain a full
cylinder.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Monday, March 27, 2000 1:59 AM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} thanks to the many responders about this post. Let me add some
} more info to help zero in on a solution--if there is one.
}
} The reason I am seeking a vacuum container is two-fold. First,
} I have very limited N2 availability. I use industrial grade bottled N2
} and dry it with a molecular sieve for venting my SEM chamber
} during specimen exchange. I vent at about 5psi. The bottles
} are at 1900 psi and hold 130cu ft of N2. One bottle lasts about
} two months. I have two bottles--on-line and standby. A "trickle"
} of N2 through a dessicator would work but for how long per
} bottle? I don't know.
}
} The other factor for a vacuum container is that if a specimen is
} under a roughing pump vacuum, if it is at all defective, the body
} of the specimen will implode. Thus saving the time of fooling with
} it in the SEM. If it does not implode, then it will be dry and not
} require very much pump down time before opening the column
} isolation valve. Thus ensuring minimal effect on the column ion
} pump.
}
} Steve D'Angelo showed a very nice metal dessicator unit, which
} would be great if I had a lot of N2....I presume a lot of N2. If
} I take one of my 130 cu ft cylinders and leak N2 into the dessicator,
} about how long will it last? And what is the minimum leak pressure
} needed to make the dessicator function as a drying unit? I could
} get another cylinder of N2 and another sieve for this unit if the
} gas volume was sufficient to run the box for two to three months.
}
} Appreciate your feedback.
}
} gary g.
}
}



From daemon Mon Mar 27 07:34:51 2000



From: BARRY SHAW :      Barry.Shaw-at-nottingham.ac.uk
Date: Mon, 27 Mar 2000 13:46:37 GMT0BST
Subject: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

Does anyone no of a good web source for info on light microscopy for
undergraduates. Ideally I'm after reference material on brightfield,
phase contrast and fluorescent microscopies. Some info on new areas
would also be useful. Hope someone out there can help !

Thanks in Advance,

Barry Shaw



Barry Shaw
Senior Imaging Technician
Molecular & Cell Biology
E Floor
School Of Biomedical Sciences
University of Nottingham Medical School
NG7 2UH



From daemon Mon Mar 27 08:07:10 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 27 Mar 2000 07:37:35 -0600
Subject: Nomarski DIC

Contents Retrieved from Microscopy Listserver Archives
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Dear All:

I am looking to purchase an Zeiss INKO condenser with 4 Wollaston prisms.
The turret position/Wollaston prism marked IIII was used in conjunction
with the Plan Achromat 6.3x objective.

What was the difference between the INKO condenser (46 52 79) and the INKO
slider III (47 44 44) and INKO slider II (47 44 31)? According to the
literature, the type II INKO slider was for research microscopes and the
type III for the the smaller STANDARD microscope.

Using a Photom I, I have tried the INKO condenser in position IIII with
both type III and type II sliders and am not able to obtain DIC with a
PLAN achromat x6.3 objective.

I am very familiar with the 3 position Wollaston prism condenser and am
able to obtain great interference DIC with position I and the PLAN x16
objective. However, using the 4 position condenser on prism IIII yields
no DIC using the x6.3 objective. As a side note, the prisms are in great
shape and show no sign of separation.

I have noticed the dark fringe interference figures for IIII, III, and II
are oriented at 11:00 and 5:00 when viewed through an inverted condenser,
while in position I, the dark fringe is oriented at 1:00 and 7:00.
(hand held with the condenser inverted, the polarizer is placed on top of
the dove-tailed retainer ring to position and lock the condenser into the
condenser holder, and the INKO slider is held at 45 degrees to the east
-west oriented polarizer under the top condenser lens element, i.e., the
entire system is inverted.) If this makes sense, great!

Any suggestions as to why the number IIII position does not yield DIC
using the 6.3 objective?

Thanks
Ken
---------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Dept. of Biology
5000 N Willamette Bldv.
Portland, OR 97303





From daemon Mon Mar 27 08:07:10 2000



From: MGMANDERS-at-aol.com
Date: Mon, 27 Mar 2000 07:36:22 -0600
Subject: NEED TO PURCHASS: MICRO-HARDNESS TEST

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I NEED TO BUY A MICRO-HARDNESS TESTER (USED) WHICH READS OUT IN ROCKWELL OF
COURSE, IN GOOD WORKING CONDITION.

PLEASE SEND INFO TO mgmaders-at-aol.com

Please help need find one to purchass

Michael G. Manders
EM Technology Inc.
(920)262-8380




From daemon Mon Mar 27 08:07:10 2000



From: MGMANDERS-at-aol.com
Date: Mon, 27 Mar 2000 07:36:44 -0600
Subject: Correction on need to purchass (used) metallurgical hardness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Need to purchass used Metallurgical hardness tester which reads out in
Rockwell. In good working order which must be able to calbrate.

Please help me to find one to purchass send info to mgmanders-at-aol.com

Michael G. Manders
EM Technology Inc.
(920)262-8380




From daemon Mon Mar 27 08:07:10 2000



From: Ping Li :      pli-at-is.dal.ca
Date: Mon, 27 Mar 2000 09:38:08 -0400
Subject: Info on buying carbon rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I am looking for carbon rods with spectrographic quality for Edwards
vacuum evaporator. The rods should be about 1.5mm in diameter and 30mm
in length. I have tried several suppliers without success. If any of you
have information regarding who supplies this kind of carbon rods could
you please let me know? Your help will be greatly appreciated. Thank
you.

Ping




From daemon Mon Mar 27 17:24:06 2000



From: Andrea Weisberg :      AWeisberg-at-niaid.nih.gov
Date: Mon, 27 Mar 2000 11:55:58 -0500
Subject: FW: Chesapeake Society for Microscopy -Photoshop Workshop

Contents Retrieved from Microscopy Listserver Archives
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} Chesapeake Society for Microscopy
}
} Is pleased to announce
}
} The PhotoShop Workshop
}
} April 11th,2000
}
} Morning Session
} 9:00am-12: 00 noon
} Introduction and Intermediate level
}
} Afternoon Session
} 1:00pm- 4:00pm
} More Advanced Issues and Discussion
}
} Location: NIH, Building 4 Room 433
}
}
} Seating is limited to 50 people
} Preregistration for CSM members by March 31st –No Charge
} After March 31st –Open Registration
} CSM members –No charge
} Non-members - $20.00 per session
}
} To register Contact Andrea Weisberg
} Phone: (301) 435-1977
} e-mail: aweisberg-at-nih.gov
}
}
} Please register early, we can only seat 50 people.
} Ask me about how to become a CSM member
} Membership $10.00 per year
}
} Andrea S. Weisberg
} NIH/NIAID/LVD
} Bldg. 4/Room 210
} 4 Center Drive
} Bethesda, MD 20892-0445
} phone: (301) 435-1977
} fax: (301) 480-1147
} e-mail: aweisberg-at-nih.gov
}



From daemon Mon Mar 27 17:24:06 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 27 Mar 2000 08:38:13 -0800
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be very concerned about vacuum pump oil contamination of your
specimens if you us oil sealed roughing pumps on such a vacuum chamber. Oil
backstreaming will occur unless you use a trap and are meticulous about trap
maintenance.

Ronald Vane
XEI Scientific

-----Original Message-----
} From: Dr. Gary Gaugler {gary-at-gaugler.com}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com}


Hi Brian,

Service contract can be likened to "insurance policies'. We are in the service
business & offer service contracts for less mostly because we can work on
several manufacturers and save on travel time & costs. Still we allot about 20%
of the contract costs to parts.

When you "go bare" (self insure) the equipment will probably be OK for about
two years if the maintenance has been properly done. After that it is anyone's
guess.

When we offer reduced service contract prices for reduced risk (customer buys
the parts or limited number of service calls) the discount is about 20-30% to
cover parts costs. The largest expenditure is labor and parts in that order.

Competition generally helps the equation but the question that I had is when
"competition" is only done when faced with a start-up entrepreneurs.
I would ask for a further discount of at least 20%.

Regards,

Earl Weltmer
(714) 573-9158

Brian McIntyre wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} hi earl-
} i've been considering the whole service contract routine lately since i
} have an older sem, a newer fesem, and a vanilla tem under contract. the
} sum of my contracts (from the vendors) is about $40K. i have a hard time
} justifying this level of expenditure, but have been burned in the past with
} replacement part costs under "limited" arrangements. in my role i have to
} recover all lab costs, and contracts are a substantial portion of them.
}
} my main vendor (SEMs) has offered me a 5% "discount". this seems a bit
} light (to me) because i am limited in terms of my cost recovery mechanisms.
} a commercial or industrial lab would seem to have more flexibility than a
} shared facility at a small university. currently i'm negotiating with them
} to extend this discount. i'm not sure what will happen if i can't keep
} these costs under control...perhaps this is an opportunity for a crafty
} entrepreneur. at the very least it may be a signal to look for 3-rd party
} service, in-house methods, and/or go bare on older equipment.
}
} brian
} ********************
} } OK to rephase: How many have experienced an extreme reduction in service
} } contract price (20% or more) when confronted with competition?
} }
} } Thank You,
} }
} } Earl Weltmer
}
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875



From daemon Mon Mar 27 17:24:13 2000



From: DKITTLESON-at-PILLSBURY.COM
Date: Mon, 27 Mar 2000 15:35:05 -0500
Subject: Cambridge S240 SEM Available in Mid- April

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





A Cambridge S240 scanning electron microscope is available to any interested
party for moving expenses and best offer. Please contact me directly.
Thank you and kind regards,
Diana

Diana L. Kittleson
Pillsbury Technology East
737 Pelham Blvd.
St. Paul, MN 55114
651-917-5859
651-917-5850 fax
www.tpclabs.com





______________________________________________________________________
This e-mail and any attachment contains information which is private and
confidential and is intended for the addressee only. If you are not an
addressee, you are not authorized to read, copy or use the e-mail or any
attachment. If you have received this e-mail in error, please notify the sender
by return e-mail and then destroy it.




From daemon Mon Mar 27 17:34:27 2000



From: D. W. McNeil :      dmcneil-at-kwiknet.net
Date: Mon, 27 Mar 2000 17:25:26 -0600
Subject: Snappy :For Gordon Couger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used a Snappy digitizer for the last 6 years with good
results. I use it with a Javelin color camera. I,m curious, how did you
get it to work with a monochrome camera?? The Snappy syncs on the color
burst portion of the video waveform. I have never been able to get a
oicture with a monochrome camera or electron microscope.




From daemon Mon Mar 27 17:34:27 2000



From: David Browning :      dbrownng-at-stargate.net
Date: Mon, 27 Mar 2000 17:26:05 -0600
Subject: In Search of PAM Interface Board for Link EDX Pulse Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir: I am looking for a PAM interface board/card which would
plug into a slot in a Personal Computer. I have a Link Analytical EDX
connected to a Hitachi S520LB SEM. The pulse processor for the EDX is a
stand alone unit which connects via a 25 pin ribbon cable to a personal
computer of older vintage i.e.. 80286 or 80386 technology. The connection
is into a PAM Interface Card. The person I purchased this unit from was
unable to locate the PAM Interface Board. I am looking for this board.
Does anyone know where I can locate one? Thank You, David Browning
dbrownng-at-stargate.net




From daemon Tue Mar 28 06:41:45 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 27 Mar 2000 18:47:03 -0600
Subject: Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me what the "street value" might be of this SEM?

Used, unknown condition, but not trashed.

gary g.



From daemon Tue Mar 28 06:41:51 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Mon, 27 Mar 2000 22:00:18 -0800
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary:
Why not store samples in a standard vacuum desiccator (Fisher Scientific,
VWR, etc). They come in glass (with ground glass or o-ring seals) and
plastic (with o-ring seals). However, don't leave samples in a desiccator
under active pumping. All pumps backstream. It's just a matter of how
much contamination your samples can tolerate. Pump the desiccator to some
nominal reduced pressure (a few seconds is fine) and close the valve. A
silica gel or similar desiccant can be used concurrently in the dessicator
and will more effectively pump residual water out of your samples at this
reduced pressure.

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
Sent: Sunday, March 26, 2000 7:59 AM
To: MSA listserver
Cc: Steve D'Angelo


thanks to the many responders about this post. Let me add some
more info to help zero in on a solution--if there is one.

The reason I am seeking a vacuum container is two-fold. First,
I have very limited N2 availability. I use industrial grade bottled N2
and dry it with a molecular sieve for venting my SEM chamber
during specimen exchange. I vent at about 5psi. The bottles
are at 1900 psi and hold 130cu ft of N2. One bottle lasts about
two months. I have two bottles--on-line and standby. A "trickle"
of N2 through a dessicator would work but for how long per
bottle? I don't know.

The other factor for a vacuum container is that if a specimen is
under a roughing pump vacuum, if it is at all defective, the body
of the specimen will implode. Thus saving the time of fooling with
it in the SEM. If it does not implode, then it will be dry and not
require very much pump down time before opening the column
isolation valve. Thus ensuring minimal effect on the column ion
pump.

Steve D'Angelo showed a very nice metal dessicator unit, which
would be great if I had a lot of N2....I presume a lot of N2. If
I take one of my 130 cu ft cylinders and leak N2 into the dessicator,
about how long will it last? And what is the minimum leak pressure
needed to make the dessicator function as a drying unit? I could
get another cylinder of N2 and another sieve for this unit if the
gas volume was sufficient to run the box for two to three months.

Appreciate your feedback.

gary g.




From daemon Tue Mar 28 06:41:58 2000



From: Ulrike Zeile :      ulrike.zeile-at-bal-tec.com
Date: Tue, 28 Mar 2000 09:51:05 +0200
Subject: AW: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Parthasarathy,

Balzers Union renamed to BAL-TEC AG in 1992. But the product line was
maintained and new developments are carried out. There are several coating
systems of this older type in use. So of course we can deliver these targets
furthermore.
Article No. of this target: BU 007 129 -T

Our reprepresentative in USA / Canada:

Techotrade International
7 Perimeter Road
Manchester, NH 03103-3343
Mr. Johnny Hagen
T: +1 603 622-5011

Our representative will contact you directly to assist with further
information.

Ulrike Zeile


BAL-TEC AG
EM-Technology and Application
FL-9496 Balzers
Tel. +423 388 12 36
Fax +423 388 12 60


-----Ursprüngliche Nachricht-----
Von: Mandayam V. Parthasarathy [mailto:mvp2-at-cornell.edu]
Gesendet: Freitag, 24. März 2000 21:22
An: Microscopy-at-sparc5.microscopy.com
Betreff: Balzers-Union putter coater


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone have for sale, or know of a supplier of Au/Pd target for the
older model Balzers-Union Sputtering Device Type 07120-A, serial number
235? It is an early version of that coater series and uses targets for an
SCD010 type coater with serial numbers 101-317. The target is ~5 cm
diameter with a threaded mounting hole. Thanks!


*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Tue Mar 28 06:41:59 2000



From: Ulrike Zeile :      ulrike.zeile-at-bal-tec.com
Date: Tue, 28 Mar 2000 10:04:40 +0200
Subject: WG: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Mr. Parthasarathy,

Balzers Union renamed to BAL-TEC AG in 1992. But the product
line was maintained and new developments are carried out.
For additional information have a look at our webside www.bal-tec.com.
There are several coating systems of this older type in use.
So of course we can deliver these targets furthermore.
Article No. of this target: BU 007 129 -T

Our reprepresentative in USA / Canada:

Techotrade International
7 Perimeter Road
Manchester, NH 03103-3343
Mr. Johnny Hagen
T: +1 603 622-5011

Our representative will contact you directly to assist with
further information.

Ulrike Zeile


BAL-TEC AG
EM-Technology and Application
FL-9496 Balzers
Tel. +423 388 12 36
Fax +423 388 12 60


-----Ursprüngliche Nachricht-----
Von: Mandayam V. Parthasarathy [mailto:mvp2-at-cornell.edu]
Gesendet: Freitag, 24. März 2000 21:22
An: Microscopy-at-sparc5.microscopy.com
Betreff: Balzers-Union putter coater


-------------------------------------------------------------
-----------

of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-------------------------------------------------------------
----------.
++
++
++ Does anyone have for sale, or know of a supplier of Au/Pd
++ target for the
++ older model Balzers-Union Sputtering Device Type 07120-A,
++ serial number
++ 235? It is an early version of that coater series and uses
++ targets for an
++ SCD010 type coater with serial numbers 101-317. The target is ~5 cm
++ diameter with a threaded mounting hole. Thanks!
++
++
++ *******************************************************************
++
++ M.V. Parthasarathy
++ Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
++ Director, Cornell Integrated Microscopy Center (CIMC)
++ Section of Plant Biology
++ 228 Plant Science Building
++ Cornell University, Ithaca, NY 14853
++ E-Mail: mvp2-at-cornell.edu
++ Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
++ Plant Biology Fax: 607-255-5407
++ CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
++ CIMC Office Fax: 607-253-3803
++ CIMC web site: http://www.cimc.cornell.edu
++
++
++



From daemon Tue Mar 28 06:42:14 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 28 Mar 2000 10:30:00 +0100 (GMT Daylight Time)
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin blocks

Contents Retrieved from Microscopy Listserver Archives
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Like Malcolm I gave up on a hand held vacuum cleaner when
sawing resin as I suspected it leaked fine particles. Now
I saw up resin in a large bag in a fume cupboard. The bag
has lasted 5 years. Anyone got a neater/safer method?

Dave



On Mon, 27 Mar 2000 10:35:33 +0100 Malcolm Haswell
{malcolm.haswell-at-sunderland.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greg
}
} you mention using a small vacuum cleaner to clean up resin dust. You
} don't specify whether it has an exhaust filter so I think I should just
} mention that the typical domestic vacuum cleaner exhausts fine
} particulates and may well make the problem worse because it may be
} pumping the very size of resin particles out that you want to avoid. We
} once used a small portable vacuum cleaner for cleaning up resin dust but
} stopped when I realized that there was a potential risk.
}
} I know that there are now domestic and industrial vacuum cleaners which
} have very fine exhaust filters and there are also the small
} photocopier/toner vacuum cleaners. Has anyone investigated their use for
} resin dust?
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscopy
} School of Sciences
} Fleming Building
} University of Sunderland
} SUNDERLAND SR1 3SD
} Tyne and Wear
} UK
}
} Tel (0191) 515 2872
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
} greg erdos wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } We solved the resin dust/ Dremel tool problem by rigging a small vacuum
} } cleaner with the intake very close to where we would be grinding, which was
} } usually under a disecting scope. I got the idea while watching a cast
} } being cut from a broken foot. The cast cutter had a vacuum attached.
} } Our rig takes care of the fine particles, while larger ones generally fall
} } to the earth and can be cleaned up later.
} } This more of an issue with epoxies, I suspect. LR White is a dental resin,
} } I believe, and the dentist will grind in your mouth or over you using your
} } chest as a table. I don't know if that means it is safe.
} }
} } Greg Erdos
} } University of Florida
} } Greg Erdos
} } 5410 SE 185th AVe
} } Micanopy, FL 32667
} } 352-466-0843
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Mar 28 18:57:09 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 28 Mar 2000 08:47:09 -0500 (EST)
Subject: Problem with Hummer VII sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a Hummer VII sputter coater, which has an automatic processing
cycle. As the vacuum drops from 60 millitorr to 40 millitorr (during
which time the high voltage should switch on), the entire process shuts
down. I have tried running the system with the argon valve open and shut.

Any suggestions from users out there?

Thanks,

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Tue Mar 28 18:57:13 2000



From: Andy Horsewell :      horsewell-at-ipt.dtu.dk
Date: Tue, 28 Mar 2000 16:26:49 +0200
Subject: Re: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Does anyone no of a good web source for info on light microscopy for
} undergraduates. Ideally I'm after reference material on brightfield,
} phase contrast and fluorescent microscopies. Some info on new areas
} would also be useful.

Barry,
Try looking at these web sites. The virtual microscopy is fun. You might
want to filter out the stuff of interest to materials and geological
sciences... or leave it in in the name of interdisciplinary microscopy !

Andy

http://micro.magnet.fsu.edu/primer/index.html
http://micro.magnet.fsu.edu/primer/techniques/index.html
http://www.people.virginia.edu/~jaw/mse310l/w4/mse4-1.htm
http://micro.magnet.fsu.edu/primer/virtual/virtual.html
http://spm.aif.ncsu.edu/aif/om.htm




From daemon Tue Mar 28 18:57:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 28 Mar 2000 10:51:47 -0500
Subject: Re: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barry,

A number of universities are now using "Optimizing Light Microscopy for
Biological and Clinical Laboratories". It covers all the areas you
mentioned and more. It also includes a number of small experiments which
can be done as a group or on independent study. See our website for
further info.

Caveat: MME does have financial interest in this book.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 01:46 PM 3/27/00 GMT0BST, BARRY SHAW wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Mar 28 18:57:16 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 28 Mar 2000 08:41:14 -0800
Subject: Re: Info on buying carbon rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ping,
The last time I went looking for spec-pure carbon rods, our Stores man found
them at: ultra carbon, 900 Harrison St., Bay City, MI48708-8244. They
weren't that exact size, but they are a good place to try.
At 09:38 AM 3/27/00 -0400, you wrote:

}
} Hi, I am looking for carbon rods with spectrographic quality for Edwards
} vacuum evaporator. The rods should be about 1.5mm in diameter and 30mm
} in length. I have tried several suppliers without success. If any of you
} have information regarding who supplies this kind of carbon rods could
} you please let me know? Your help will be greatly appreciated. Thank
} you.
}
} Ping
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Mar 28 18:57:16 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 28 Mar 2000 08:54:46 -0800 (PST)
Subject: Re: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Does anyone no of a good web source for info on light microscopy for
} undergraduates. Ideally I'm after reference material on brightfield,
} phase contrast and fluorescent microscopies. Some info on new areas
} would also be useful. Hope someone out there can help !
}
} Barry -

I can suggest a good CD-ROM: Pagliaro, l., et al., 1997
Microscopy-Tutor. You'll find a description and ordering information in
the Project MICRO bibliography (URL below).

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Tue Mar 28 18:57:29 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Mar 2000 16:24:48 -0500
Subject: Re: TEM, Biological, sinking of tissue in sucrose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marie E. Cantino wrote:

} Here is a question for those of you who use sucrose to provide
} cryoprotection for tissues:
}
} We are preparing perfusion fixed brain tissue by freeze substitution for
} post-embedding immuno gold. Following fixation we prepare 500 micron
} vibratome sections, which we then wish to infiltrate with 2 M sucrose in
} buffer prior to freezing. We have been transferring the brain sections to
} 1 M sucrose, then when they sink, transferring again to 2 M. The problem
} is that the sections never sink in the 2 M sucrose.
}

Dear Marie,
The Handbook of Chemistry and Physics gives a specific gravety
for 2 M sucrose of about 1.25. Is the sp. gr. of your tissue (exclusive of
the exchangable fluids, of course) greater than this? If not, the sections
will never sink. If it is marginally greater, then the sections will sink
when hell freezes over, and you can do cryo easily. If you can determine
the weight of a tissue section of known volume first with fixation fluid,
then after infiltration with 1 M sucrose (and also measure the volume
change), you could extrapolate to the situation for 2 M sucrose (or some-
one with too much time on his/her hands could do this for many sucrose
concentrations). Good luck.
Yours,
Bill Tivol



From daemon Tue Mar 28 18:57:30 2000



From: Karen Kelley :      klv-at-biotech.ufl.edu
Date: Tue, 28 Mar 2000 16:42:34 -0500
Subject: localization of zinc in tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need to localize zinc in prostate tissue for TEM. Has anyone used
Sulfide-silver method or Zinc-dithizonate method? If so, is there a method
better than the other or new methods that might work on such tissue?

I am also trying to locate a reference by Pihl E. Falkmer S: Trials to
modify the sulfide-silver method for ultrastructural tissue localization of
heavy metals. Acta Histochem 27:34-41, 1967. If you have a copy of this
method please email me.

Thank you


Karen Kelley
Senior Electron Microscopist
UF Biotechnology Electron Microscopy Core Lab
Box 118525 Gainesville Florida
lab:352-392-1184 fax: 352-846-0251
email: klv-at-biotech.ufl.edu



From daemon Tue Mar 28 18:57:31 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Mar 2000 17:11:25 -0500
Subject: Re: Required help to etchquartz

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


RamaSubrahmanyam K wrote:

} I had done an evapouration of cobalt on a quartz sample. Now I want to
} etch the quartz sample to remove the substrate so that I can do
} Transmission Microscopy for charactrization. My problem is that If I etch
} the substrate with HF will it be harmful to carbon? If it is so what are
} the other materials that can be used to etch quartz.

Dear Ramu,
Quartz can be etched--slowly--in strong base. A 5-to-10 M
solution of NaOH should do the trick, but if the quartz thickness is in
the mm range, it will take a long time. I don't know whether the base
will be harmful to either carbon or cobalt, but I don't think it will.
Good luck.
Yours,
Bill Tivol



From daemon Tue Mar 28 18:57:32 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Mar 2000 17:19:36 -0500
Subject: Re: Info on buying carbon rods

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Ping Li wrote:

} Hi, I am looking for carbon rods with spectrographic quality for Edwards
} vacuum evaporator. The rods should be about 1.5mm in diameter and 30mm
} in length. I have tried several suppliers without success. If any of you
} have information regarding who supplies this kind of carbon rods could
} you please let me know? Your help will be greatly appreciated. Thank
} you.
}

Dear Ping,
I got mine from Ted Pella, but I'm surprized you didn't find
them
in other suppliers' catalogues. Pella lists both carbon and graphite, both

spectroscopically pure and technical grade, in several diameters and
lengths.
I have no connection to Ted Pella, Inc. except that of customer.
Yours,
Bill Tivol



From daemon Tue Mar 28 18:57:35 2000



From: C. Singla :      csingla-at-uvic.ca
Date: Tue, 28 Mar 2000 15:03:00 -0800
Subject: Preparation of tissue culture cells for TEM

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Hello everyone,
I have a student who wants to prepare tissue culture cells for TEM in Agar
blocks. I shall appreciate any suggestions.
Thanks in advance.
C.L.Singla



From daemon Tue Mar 28 21:05:15 2000



From: Keith Moulding :      mcmouldk-at-ust.hk
Date: Wed, 29 Mar 2000 09:16:05 +0800
Subject: Analytical TEMS - The choices

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Greetings,

We are contemplating replacing our analytical TEM. A basic spec we envisage
is STEM, EDS + GIF/PEELS. We are happy to choose the EDS, but we run into
trouble with e-guns, i.e. to go LaB6 or FEG. In particular as we would like
to bolt a GIF/PEELS on to the system.

We have several concerns:
The safety of the FEG gun, considering we are a multi-user facility with
users who have a frequent tendency to bend holders and press the wrong
buttons. How problematic are FEGs, do you have much down time / running
cost due to careless users?

GIF/PEELS: has anybody carried out a systematic comparison between a system
with LaB6 or FEG. Is the extra cost of a FEG (over twice the price) really
justified for GIF/PEELS or will it quite happily run on a LaB6.

The thoughts of fellow microscopy in the same quandary much a appreciated.

Keith.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Facility
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Mar 28 21:15:21 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 28 Mar 00 23:59:41 -0800
Subject: Re: TEM, Biological, sinking of tissue in sucrose

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Sucrose infiltration is an essential step in the preparation of cryosections for immunocytochemistry. Aldehyde-fixed biological tissue is infiltrated in 2.0M to 2.3M sucrose to protect the sample from freezing damage when it is subseuently frozen by immersion in liquid nitrogen. If the sample is fixed, the membranes become permiable to sucrose and the sample can be frozen into a vitreous state by immersion in liquid nitrogen. Although it is advised to infiltrate for 24 hr on a rotator, many years of experience has demonstrated that even after 15 min of exposure to sucrose, small pieces of aldehyde-fixed material are sufficiently cryoprotected to allow for successful vitrification. Take no heed of whether the sample has sunk to the bottom of the tube or not, check to see if it has been fixed and has spent sufficient time in the sucrose. If it has, it will be cryoprotected.

Useful reference:
Griffiths, G, McDowall, A, Back, R, Dubochet, J. 1984. On the preparation of cryosections for immunocytochemistry. Ultrastruct. Res. 89:65-78
They showed by quantitative mass measurements that fixed cells are freely permeable to sucrose.

Best regards,

Paul Webster

Paul Webster, Ph.D.
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Bill Tivoli wrote:
The Handbook of Chemistry and Physics gives a specific gravety
for 2 M sucrose of about 1.25. Is the sp. gr. of your tissue (exclusive of
the exchangable fluids, of course) greater than this? If not, the sections
will never sink. If it is marginally greater, then the sections will sink
when hell freezes over, and you can do cryo easily. If you can determine
the weight of a tissue section of known volume first with fixation fluid,
then after infiltration with 1 M sucrose (and also measure the volume
change), you could extrapolate to the situation for 2 M sucrose (or some-
one with too much time on his/her hands could do this for many sucrose
concentrations). Good luck.

Marie E. Cantino wrote:
We are preparing perfusion fixed brain tissue by freeze substitution for
post-embedding immuno gold. Following fixation we prepare 500 micron
vibratome sections, which we then wish to infiltrate with 2 M sucrose in
buffer prior to freezing. We have been transferring the brain sections to
1 M sucrose, then when they sink, transferring again to 2 M. The problem
is that the sections never sink in the 2 M sucrose.





From daemon Wed Mar 29 07:35:11 2000



From: christine richardson :      a.c.richardson-at-durham.ac.uk
Date: Wed, 29 Mar 2000 09:41:16 +0100
Subject: critical point drying apparatus

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Hi,
Our safety people are concerned about having our Polaron c.p.d
pressure tested at regular intervals.
Does any one out there know of anyone who does this sort of thing,
preferably on site?
Also I would be interested to hear how other users safety check this
sort of equipment.
thanks,
Christine.



From daemon Wed Mar 29 07:35:12 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Wed, 29 Mar 2000 10:24:56 +0100
Subject: TEM:Analytical TEMs

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Dear Keith,
If you are seriously worried about the experience of some of the users
then you should consider the Philips/FEI Tecnai series of instruments. The
person in charge of the microscope can determine the level of access to all
the features for each user i.e. basic, medium and expert user profiles. The
microcope, in a similar way to fly-by-wire aircraft, will simply prevent
users from blowing the tip up or anything else detrimental to the 'scope.
This should take out the worry about user/FEG problems.

The LaB6 and FEG work identically with the GIF/PEELS, they're only
electrons after all. The FEG will give you a smaller energy width (good for
EELS:ELNES .etc) and more current (good for Energy Filtered TEM,
STEM:HAADF, EELS & EDS).
Then there is the option of adding a biprism with the FEG if you want to
try off-axis holography.
The FEG machine will simply give you a wider tolerance range in terms of
specimen thickness and experimental conditions i.e greater signal to noise
ratios than with the LaB6, something that is a problem with STEM based
methods. FEG alignment procedures will be slightly different too.

We have taken delivery of a 300kV Tecnai and I have to say that the machine
is very impressive. The machine is very stable and the high brightness FEG
is a major improvement over the LaB6. The GIF is also quite a God-send for
zero-loss filtering and for mapping.

I hope this helps, Jon

P.S. I am not affiliated or have any commercial connection to Philips/FEI.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Wed Mar 29 07:35:13 2000



From: rajdeep-at-aripune.ernet.in (Rajdeep Dongre)
Date: Tue, 28 Mar 00 18:04:32 PST
Subject: Pirani Gauge of Oxford Hexland - CT1000.

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Friends,

The Pirani Gauge of the Oxford Hexland - CT1000 cryo unit mounted on SEM
S120 is not working. Can anyone guide me in purchase of this? Could
this be manufactured in-house? Please help me out.

Rajdeep Dongre
Electron Microscopy Laboratory
Agharkar Research Institute
G.G. Agarkar Road
Pune - 411 004, India
Phone 91-20-5653680/5654357
e-mail : rajdeep-at-aripune.ernet.in



From daemon Wed Mar 29 07:35:17 2000



From: Anne Heller :      heller-at-Uni-Hohenheim.DE
Date: Wed, 29 Mar 2000 14:23:00 +0200
Subject: Epon sections

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Message-ID: {38E1F5A4.96CAA8EC-at-uni-hohenheim.de}


We work with plant material and after ultra thin sectioning our sections
show lines of compressions in cell wall areas. Of course, we try to
stretch the Epon sections with a heat pen or with chloroform, but very
often we are not successful. The sections just move on the water
surface and nothing is happening. Any advice would be gratefully
appreciated.
Regards,
Anne Heller
--
Dr. Anne Heller, AG Elektronenmikroskopie,
Institut fŸr Botanik (210), UniversitŠt Hohenheim,
Garbenstra§e 30
D-70593 Stuttgart

Tel.: (0049)-711-459-2180
Fax.: (0049)-711-459-3355




From daemon Wed Mar 29 17:30:29 2000



From: GuessWho :      olivier.balmes-at-oorg2.lth.se
Date: Wed, 29 Mar 2000 16:26:58 +0200
Subject: Re: EFTEM/GIF on particles

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Hi Jo,
Thanks for your answer.
We use our GIF in a similar way, focusing around the energy loss we want to
take the image at with a slit width covering the 3 energy windows if possible.
This works best for rather thick samples (compared to our 5 nm particles)But
as you say, maybe it's just the spatial resolution which is not good enough,
our microscope was designed to enhance contrast for biological studies,
unfortunately loosing some resolution in the process. We are not yet trying to
get concentrations out of the pictures...rather get a qualitative mapping.

I tried to do EELS measurements a few times and got very few succes. The best
results are obtained on beam resistant samples simply because I can then move
the zero loss peak off the CCD and increase intensity (or acq time) at will. I
start with a very low intensity and optimise it in turboview mode to get a
good signal out. However I could seldom see well defined peaks.
One point which I find strange is that we can often get reasonnably good
elemental maps even when we don't see any peak in the EELS spectrum. The
question is then if the map is really trustworthy...

Any comments welcome !

Olivier



Jo Verbeeck wrote:

} Hello,
}
} Regarding EFTEM on small particles, this should actually work quite well.
} That is, elemental mapping works reasonably if you choose the right
} objective apperture. You should simulate the spatial resolution to see
} what can be attained under your conditions (HT, Cc, Cs etc).
} However it will be very difficult to get real concentration information
} out of these images. I'm trying EELS with nanoprobe but so far no succes
} (everything gets destroyed before I take a spectrum, allignment is very
} very difficult)
} Still, focussing remains a problem. Comon practice is to focus at 100eV
} and then suppose everything is OK for higher losses. I do not understand
} the theory of this technique (blurring by finite slitwidth & Cc?) nor thus
} it work well. Any comments on this are welcome.
}
} Jo
}
} *************************************************************
} * Jo Verbeeck *
} * University of Antwerp *
} * Dept. EMAT (Electron Microscopy for Materials Research) *
} * e-mail: joverbee-at-ruca.ua.ac.be *
} * tel: +32(0)3 218 02 49 *
} * fax: +32(0)3 218 02 57 *
} *************************************************************
}
} On Tue, 21 Mar 2000, GuessWho wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear all,
} }
} } I was wondering if anybody was using a GIF filter. We have one mounted
} } on a 120 kV TEM and are trying to image small particles (5-10 nm) and
} } doing some energy filtering to image different elements in the particles
} } (for example gold, silicon, copper, cerium...) . This is not really what
} } I would call trivial work and we are still working out the set up
} } (window size and positionning for example) in a rather empiric way. The
} } main problem is often to get a decent signal in the interesting energy
} } region and still keep the windows fairly narrow.
} } I would add that we often have to work at low dose because of the beam
} } sensitivity of our samples, but I guess our GIF setups can be improved.
} } Any suggestion ?
} }
} } Any general comment on the GIF warmly welcome !
} }
} } Thanks
} }
} } Olivier
} }
} }
} }



From daemon Wed Mar 29 17:30:30 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 29 Mar 2000 09:46:56 -0800
Subject: sRe: Epon sections

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Anne Heller wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We work with plant material and after ultra thin sectioning our sections
} show lines of compressions in cell wall areas. Of course, we try to
} stretch the Epon sections with a heat pen or with chloroform, but very
} often we are not successful. The sections just move on the water
} surface and nothing is happening. Any advice would be gratefully
} appreciated.
} Regards,
} Anne Heller
} --
} Dr. Anne Heller, AG Elektronenmikroskopie,
} Institut fŸr Botanik (210), UniversitŠt Hohenheim,
} Garbenstra§e 30
} D-70593 Stuttgart
}
} Tel.: (0049)-711-459-2180
} Fax.: (0049)-711-459-3355

Hi Anne,

My first guess is that the mechanical properties of your Epon mix differs
too much from that of the fixed walls. However, when I worked with plant
material, I generally used Xylene to stretch floating sections (rarely in
Epon). I'm not sure if was the aromatic character or the boiling point
difference that made it better at swelling and relaxing the sections than
CHCl3.

Other suggestions would be using a harder resin (my favorite was VCD and
Quetol 651 (equiequivalent) hardened with NSA (or HSA), which I called
Spurtol ;-)). I found it stains easier than Spurr's yet is low viscosity
and about as hard as a medium Spurr's. Reducing the knife angle might
help. Post curing the existing blocks at elevated temperature might
harden them some more.

Cheers,

John

John Heckman
MSM Department
Michigan State University



From daemon Wed Mar 29 17:30:30 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Wed, 29 Mar 2000 09:58:03 -0400
Subject: Re: TEM:Analytical TEMs

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I don't know if you have interest in this but it is good info.
Wallace

-----Original Message-----
} From: Parker, Jo
Sent: Wednesday, March 29, 2000 8:45 AM
To: All SOD Faculty & Staff


Dear Jonathan and Keith,

I just want to add a few comments to what Keith said about FEG's. One of
the major advantages of a FEG over conventional souces for AEM is the spot
size. The new FEG scopes have STEM resolution of 2 angstroms with great
brightness. (I have only seen the Philips/FEI scope, but I think the other
companies have similar specs.) When one is deciding whether or not to go
for the FEG, I think it will depend on your applications. If a small
intense beam and good energy resolution are necessary, go for the FEG. Of
course, there is also the consideration of the cost of service contract
($$$$).

Ciao for now,
Ken




From daemon Wed Mar 29 17:30:30 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 28 Mar 2000 10:09:25 -0500
Subject: CPD safety question

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Christine Richardson wrote:
===================================================
Hi,
Our safety people are concerned about having our Polaron c.p.d
pressure tested at regular intervals.
Does any one out there know of anyone who does this sort of thing,
preferably on site?
Also I would be interested to hear how other users safety check this
sort of equipment.
===================================================
I might not be the last word on this, but I had described to me the kind of
large liquid filled tank (I think it is water) that pressure vessels like
this are tested in, and to me at least it did not sound like it would be the
kind of thing that one would "carry around" in a portable kind of unit to do
this kind of testing, on site. This description came, some years ago, from
Dr. Wilf Gee (now deceased) and who was very much involved in the original
manufacturing of this product and who had involvement for many years with
the safety testing of the unit.

When your CPD unit was first delivered, it would have come with a copy of a
report from the testing agency showing the results and conditions of the
pressure testing. I might be off by a bit on this, but it was my
recollection that the vessel is tested to a pressure level that is three or
four times higher than what would be required to blow out the rupture disc.
In other words the unit is tested to a level that, if the rupture disc did
not blow exactly where it was supposed to blow, there is some very large
margin of error so that the disc still would blow long before the vessel.

There are probably more CPD units of this design installed in the world than
any other design. I have never heard of anyone having any kind of a problem
with it (e.g. an explosion), except an occasional blowing of a rupture disc,
but certainly not the pressure vessel itself. Just don't ever try to run
the unit by replacing the rupture disc, if one is not handy, with a cut
metal disc. That is very dangerous and should never be done. Believe it or
not, we do uncover once in a while, people who do do that sort of thing, out
of naivety of course and that is why I do not waste an opportunity to point
out the danger in doing that sort of thing. That is why I always have
recommended, for multi-user environments in particular, to always have a
small supply of replacement rupture discs near by the unit just to avoid
even the slightest temptation (such as by a student) to by-pass this
important safety feature.

So I for one would be very interested in hearing the logic and rationale of
your safety committee that is of the opinion that you should have your
pressure vessel tested from time to time.

However, there is one kind of situation where one should have the vessel
pressure tested and that is if one has attempted any mechanical
modifications to the chamber itself. Then the system should be pressure
tested. And since the manufacturer of this particular CPD unit (e.g.
Polaron) has had on the order of thirty years of experience doing this kind
of testing, on this particular design of vessel, I would recommend having
them do the testing for you in the UK. Today the testing of the Polaron
unit is done to the CE standard, without doubt the most stringent in the
world.

If you do not have the original test certificate, if you send me your S/N I
could try to get a copy of it for you. That alone might satisfy the
questions being asked of your safety people.

Disclaimer: SPI Supplies has offered this particular CPD unit, especially
to customers outside the USA, for nearly twenty five years and we are
unaware of any requirement to have the vessel retested at periodic intervals
for safety reasons.

Chuck


============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Wed Mar 29 17:30:32 2000



From: Derrick Calandra :      calandra-at-atfinet.com
Date: Wed, 29 Mar 2000 09:59:59 -0600
Subject: seeking equip.

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I'm looking for some used equipment to set up a small lab space. Any
recommendations would be great.

1. inverted microscope, for TEM sample prep
2. hot plate
3. diamond saw

Thanks in advance.

Derrick



From daemon Wed Mar 29 17:30:33 2000



From: Ginger R Hendricks :      lizard-at-osu-com.okstate.edu
Date: Wed, 29 Mar 2000 10:15:43 -0600
Subject: Immunogold enhancement method questions

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Hello all,

I am passing this on for a fellow colleague. Thank you in advance.

Ginger (Baker) Hendricks
EM Lab Manager
Oklahoma State University College of Osteopathic Medicine


"I am looking for an immunogold-silver enhancement method for labelling a
cytoplasmic antigen in cultured cells and viewing with Light Microscopy.
We will be using Protein Aand a monoclonal antibody. Once we have
determined that we have specific labelling, we will proceed to visualizing
the gold with TEM."



From daemon Wed Mar 29 17:30:36 2000



From: Dr. Tina Schwach :      tschwach-at-barrishind.com
Date: Wed, 29 Mar 2000 11:45:23 -0600
Subject: Re: Preparation of tissue culture cells for TEM

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} Hello everyone,
} I have a student who wants to prepare tissue culture cells for TEM in Agar
} blocks. I shall appreciate any suggestions.
} Thanks in advance.
} C.L.Singla
}
It would be helpful to know how the tissue culture cells are being
grown...in plastic wells where they will be scraped off or on plastic or
glass coverslips where the entire surface will be embedded. What is the
ultimate goal of the TEM examination...morphology, interactions? Will the
samples be ultimately embedded in plastic or expoxy?

Dr. Tina Schwach
Microscopy Consulting Services, Inc.



From daemon Wed Mar 29 17:30:36 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Wed, 29 Mar 2000 12:01:41 -0600
Subject: SEM and TEM of spores

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I would appreciate it very much if someone can refer me to a good book or
protocol for preparation of fungal spores for SEM and TEM. This is very
foreign to me since I have been working with mammalian tissues exclusively
for so many years but now that the lab is a core facility I am getting somel
requests for other types of samples. Thank you,

Cora Bucana
*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Wed Mar 29 17:30:37 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 29 Mar 2000 13:37:03 -0800
Subject: Re: critical point drying apparatus

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christine richardson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
} Our safety people are concerned about having our Polaron c.p.d
} pressure tested at regular intervals.
} Does any one out there know of anyone who does this sort of thing,
} preferably on site?
} Also I would be interested to hear how other users safety check this
} sort of equipment.
} thanks,
} Christine.

Hi Christine,

Over here in the US we have an Interstate Commerce Commission that checks
pressure vessels via hydrostatic testing. This is required every 5 years
and the tops of gas cylinders, at least, are stamped with the last test
date. It's always amazed me just how long N2 cylinders last (reading
hydrostatic test dates is something to do while your plates are
processing). I've seen bottles with dates that were before W.W.I. All that
age and 2250 psi. I've never heard of them requiring the testing of lab
equipment, though. Might check with your bottled gas supplier to see if
they'd do it.

cheers,
John



From daemon Wed Mar 29 17:30:37 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 29 Mar 2000 11:46:11 -0700 (MST)
Subject: Re: Epon sections

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On Wed, 29 Mar 2000, Anne Heller wrote:

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}
} We work with plant material and after ultra thin sectioning our sections
} show lines of compressions in cell wall areas. Of course, we try to
} stretch the Epon sections with a heat pen or with chloroform, but very
} often we are not successful. The sections just move on the water
} surface and nothing is happening. Any advice would be gratefully
} appreciated.
} Regards,
} Anne Heller
} --
} Dr. Anne Heller, AG Elektronenmikroskopie,
} Institut für Botanik (210), Universität Hohenheim,
} Garbenstraße 30
} D-70593 Stuttgart
}
} Tel.: (0049)-711-459-2180
} Fax.: (0049)-711-459-3355
}
}
}
}
Hi,

If I get any reaction in a section floating in a boat from xylene, heat
pen, chloroform, etc., I know that something is wrong. My personal test
of a good embedment is nonreactivity of floating sections. Why?

In industry, if it is important that there are no free monomers in the
section, the material is exposed to the above solvents to soak them out.

If you have enough unpolymerized monomers in your section, solvents will
affect that section. (Note: Embedments that are too soft in formulations
are excluded from this discussion at this time).

So - keep your formulations well mixed at all times - do not allow it to
sit in the hood. It must be in motion at all times. Push infiltration!
More changes, longer times. If using epoxy of any sort, heat the
infiltration medium to 37 deg C for 1 hour after every new change of
resin while the sections are on the rotator. A plain 60W light bulb is
ideal for this. Polymerize well. At least for 48 hours. If needed,
repolymerize at 95 deg C for an hour especially if your formulation
contains NMA.

Your formulation may be wrong for your material. Try going harder. Also
with existing blocks, try reheating, then try a lower knife angle and a
slower speed of cutting.

Most important: Keep records. Know what you are doing and why. Do not
haphazardly try this, try that. You will get crazy! Embedments are very
complex systems that belong into the realm of material science - that is
why biologists have so much trouble with them. (I have had mega fights
lasting years with epoxies)

Good luck,

Hildy Crowley
University of Denver
Denver, CO



From daemon Wed Mar 29 17:30:44 2000



From: Alan G. Fox :      Alan.Fox-at-uts.edu.au
Date: Thu, 30 Mar 2000 08:23:19 +1000
Subject: Re: Analytical TEMS - The choices

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Keith
If you have a new FEG machine with a Schottky emitter, then these are
relatively easy to operate and fairly robust, but they do, of course, cost a
lot more and so you may not want to spend that money and have ham-fisted users
operating it. For high-resolution analytical TEM/STEM there is no doubt that
FEG is the preferred source. The small source size and small energy spread
combined with high gun brightness gives great performance. A word of
warning,though. When using a GIF with any TEM it is often necessary to operate
at low mag because of the additional mag (18 - 20 times) introduced by the
GIF. In this situation (depending on the mag), the LaB6 source may be
preferable in certain situations because the total current is higher than the
FEG source. I have used both types of machine-Schottky FEG plus GIF and LaB6
plus GIF and there do not appear to be any severe problems with the low mag
mode operating with a Schottky source. The cold FEG source may not be so good
from this point of view, since the source size is smaller than the Schottky
FEG and so the cross-over probe size above which the current in the cold FEG
probe is less than the LaB6 is small and probably in the range 10-100 nm. I
have not used a cold FEG TEM with a GIF such as the Hitachi and so I can't
really make an informed comment, but Profs Dravid and Marks at Northwestern
University are very skilled users of the Hitachi machine and they can surely
advise you. Best of luck.

Alan Fox
Center for Materials Science and Engineering
Naval Postgraduate School
Monterey
California 93940


Keith Moulding wrote:

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} Greetings,
}
} We are contemplating replacing our analytical TEM. A basic spec we envisage
} is STEM, EDS + GIF/PEELS. We are happy to choose the EDS, but we run into
} trouble with e-guns, i.e. to go LaB6 or FEG. In particular as we would like
} to bolt a GIF/PEELS on to the system.
}
} We have several concerns:
} The safety of the FEG gun, considering we are a multi-user facility with
} users who have a frequent tendency to bend holders and press the wrong
} buttons. How problematic are FEGs, do you have much down time / running
} cost due to careless users?
}
} GIF/PEELS: has anybody carried out a systematic comparison between a system
} with LaB6 or FEG. Is the extra cost of a FEG (over twice the price) really
} justified for GIF/PEELS or will it quite happily run on a LaB6.
}
} The thoughts of fellow microscopy in the same quandary much a appreciated.
}
} Keith.
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr. K. Moulding.
}
} Materials Characterisation and Preparation Facility
} Hong Kong University of Science and Technology,
} Clear Water Bay,
} Kowloon,
} Hong Kong.
}
} FAX: (852) 2358 2451
} TEL: (852) 2358 8724
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Mar 30 07:28:13 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 30 Mar 2000 10:04:24 +1000
Subject: FW: critical point drying apparatus

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Phone a divers shop and ask who is testing their cylinders. Those people can
also test the CPD. It would be a hydrostatic test and they would need to have a
fitting to connect to the back of the CPD. To have any meaning the applied
pressure would need to be higher than normally applied, I suggest by 50%.

I think its a bad idea to do such a test. The metal housing of those units is
vastly over-designed and the window is quartz which ultimately would crack but
not shatter. All the test would achieve is to burn up some of your funds and
time. Stress the system and if your unlucky crack the window - would the
"safety people" pay for that?

It would be interesting to learn how many systems have failed and if there were
any special circumstances. I don't know of any instance of a CPD failure.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com


Hi,
Our safety people are concerned about having our Polaron c.p.d
pressure tested at regular intervals.
Does any one out there know of anyone who does this sort of thing,
preferably on site?
Also I would be interested to hear how other users safety check this
sort of equipment.
thanks,
Christine.




From daemon Thu Mar 30 07:28:17 2000



From: Pnina Ari-Gur :      arigurp-at-wmich.edu
Date: Wed, 29 Mar 2000 21:26:12 -0500
Subject: Position Announcement

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ASSOCIATE DEAN, COLLEGE OF ENGINEERING AND APPLIED SCIENCES

Western Michigan University seeks applications and nominations for the
position of Associate Dean for Undergraduate Programs and Assessment of
the College of Engineering and Applied Sciences.

Western Michigan University is a Carnegie Doctoral I university with 750
FTE tenure-track faculty and an enrollment of about 27,000 students, 25%
at the graduate level. It is advancing to Carnegie Research II
classification and plans a substantial investment in the College of
Engineering and Applied Sciences, including a new physical facility, to
accomplish this goal. In addition to its Graduate College and Lee
Honors College, Western supports seven degree-granting colleges: Arts
and Sciences, Haworth College of Business, Education, Engineering and
Applied Sciences, Aviation, Health and Human Services, and Fine Arts.
These colleges offer 242 academic programs, including 60 at the masterâs
level and 25 at the doctoral level.

The Associate Dean for Undergraduate Programs and Assessment reports to
the Dean. This person provides leadership and oversees the development
of college policy for undergraduate programs, accreditation, assessment,
advising, recruitment, retention, student orientation, co-op, and intern
programs. An earned terminal degree in one of the disciplines in the
college or a related science is desirable. A doctoral degree, with at
least one degree in an engineering or closely related
science/engineering discipline, is required. University experience in
academic programs and demonstrated excellence in teaching are required.
Candidates should have excellent administrative and interpersonal skills
and experience utilizing instructional and administrative technology.
An excellent record of teaching and research and/or creative achievement
that would warrant an appointment as associate or full professor with
tenure in one of the academic units in the university is required.

The individual selected will assume academic and administrative
responsibilities for undergraduate programs in a dynamic and growing
college offering 16 baccalaureate, 11 masterâs and three doctoral
programs. The collegeâs staff includes 70 full-time faculty, 10
administrators (8 with faculty rank), 31 funded staff positions, and
dozens of contract staff and graduate student assistants, and teaches
approximately 2300 students. The college has a strong commitment to
education and research, which spans a broad spectrum of engineering and
engineering technologies. The college offers courses and programs
primarily on Western Michigan Universityâs main campus in Kalamazoo, but
also serves as a major resource for off-campus instruction and economic
growth at fives sites across West Michigan.

Candidates should submit 1) a curriculum vita, 2) three letters of
recommendation, 3) an application letter stating their qualifications
for the position, and 4) a statement outlining a personal vision for
engineering education to:

Parviz Merati, Chair
Associate Dean Search Committee
Department of Mechanical and Aeronautical Engineering
Western Michigan University
Kalamazoo, MI 49008

Review of applications will begin on or about May 1, 2000. Applications
will be accepted until the position is filled. Western Michigan
University is an equal opportunity /affirmative action employer.

For additional information about WMU and the College of Engineering and
Applied Sciences, refer to our Website: http://www.wmich.edu/.
=====================================
Pnina Ari-Gur, D.Sc., Professor
Materials Science and Engineering
CMD Department
Western Michigan University
Kalamazoo, MI 49008
(616) 387-3372 FAX: (616) 387-6517
email: pnina.ari-gur-at-wmich.edu
http://www.wmich.edu/cmd/arigur.htm
=====================================




From daemon Thu Mar 30 07:28:29 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 29 Mar 2000 20:42:05 -0600
Subject: Leaf Microlumina

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This is on ebay now. Here is the URL:

http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=296173307

Not announced on ebay, and limited to MSA, I am offering
an IBM Thinkpad 355 which is optionally available to make this camera truly
portable. $350 extra gets the Thinkpad. You will still need a Slim SCSI
PCMCIA card, but these are very low cost. I have one in a Sony VAIO
and it works great with the Leaf.

Any questions, please ask.

gary g.



From daemon Thu Mar 30 07:28:35 2000



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Thu, 30 Mar 2000 12:01:13 +0530
Subject: EM image simulation software

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I need information related to EMS software. There was a related posting (enclosed below) recently, replies to which I seem to have missed. My mail to the author of that posting also bounced back. Could somebody please help me get this info? Thanks very much.
----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----



-----Original Message-----
} From: Divakar R [SMTP:divakar-at-igcar.ernet.in]
Sent: Wednesday, March 29, 2000 8:23 PM
To: 'Chengge Jiao'


Hi,

Does anybody know the price for following softwares for EM simulation.

1. Diffraction 2.0 (or the most latest version),

2. EMS.

Chengge Jiao

H.H.Wills Physics Laboratory
University of Bristol, UK

c.g.jiao-at-bristol.ac.uk








From daemon Thu Mar 30 07:28:37 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 30 Mar 2000 08:32:25 +0100 (BST)
Subject: Re: TEM:Analytical TEMs

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Hi Keith,

In the matter of inexperienced users and FEG versus Lab6 I have no fears
on our FEGTEM.

We have been running our JEOL3000F for over a year now without any
trouble, it has a Schottky emitter so there is no flashing to be carried
out. The emitter is the original one used for factory tests, shipped
over, used for installation and still in use. We have experience of
several LaB6 instruments over the past 15-20 years and are a materials
science multi-user facility.

The FE gun runs 24 hours a day, the user just opens the valve to see the
beam. The gun and emitter are run up by an experienced person whenever it
needs it (every 2-3 months). We have a `Quick Emission Selector' that we
have set up to give 3 more emission currents (higher for GIF, lower for
less energy spread etc.) and the user cannot readjust these only select
between them. There is no room for abuse (even for some of our users!).

The LaB6 guns have to be run up at the start of every session and every
time a user changes specimen or films. After a period of time the LaB6
tips slowly deposit insulating LaB6 on the Wenhelt, this charges
and the bias has to be adjusted to reset the emission correctly. When a
user finishes the session this charge dissipates and the next user gets a
high emission current which has to be reset using the bias. The bias has
to be adjusted during the first part of the session as the charge builds
up. This gives the user lots of room for abusing the tip, it is not
possible to prevent access to the bias control as they do need to use it.

Regards,
Ron

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Thu Mar 30 07:28:37 2000



From: Gunnar Kopstad :      gunnar.kopstad-at-medisin.ntnu.no
Date: Thu, 30 Mar 2000 09:28:45 +0200
Subject: Critical point drier, window cracking

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hi,

Two days ago, the window in our CPD cracked. The run was in every respect
normal, but the window cracked at 35 degrees/80 bar. This was a big
surprise to us, as we have belived that this could never happen as long as
temperature and pressure are within their normal ranges. We would be very
interested to receive comments on this.

The week before, the equipment, which is more than 20y old, had been
disassembled for cleaning and replacement of the window O-ring.

Best Regards, Gunnar Kopstad.


Vennlig Hilsen
dr.ing Gunnar Kopstad
overingeni¿r Avd f Patologi, Rit

tlf. 73 86 86 56, tlf. privat 72 88 79 58
pers.s. 967 75 026



From daemon Thu Mar 30 07:28:38 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 30 Mar 2000 08:33:52 +0100
Subject: Re: FW: critical point drying apparatus - life of?

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For the record - and maybe this is a record - our Polaron E3000 CPDA
was tested in Feb. 1974 to a proof load of 2,500 lbf/in2. Under
"Remarks" on the test certificate it says "Proof pressure applied
without any visual signs of distortion or leakage. Safety valve set at
1,900 lbf/in2."

I take "lbf/in2" to be "pounds per square inch".

The unit has been in quite regular use since 1974 with no problems
other than failure of seals. I did once have to de-"fur" the
waterways but that was a local water supply problem.

Keith Ryan
Marine Biological Association
Plymouth UK



From daemon Thu Mar 30 07:28:39 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Thu, 30 Mar 2000 10:19:20 +0200
Subject: Re: EFTEM/GIF on particles

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At 16:26 29.03.00 +0200, you wrote:

..

} I tried to do EELS measurements a few times and got very few succes. The best
} results are obtained on beam resistant samples simply because I can then move
} the zero loss peak off the CCD and increase intensity (or acq time) at
will. I
} start with a very low intensity and optimise it in turboview mode to get a
} good signal out. However I could seldom see well defined peaks.
} One point which I find strange is that we can often get reasonnably good
} elemental maps even when we don't see any peak in the EELS spectrum. The
} question is then if the map is really trustworthy...
}
} Any comments welcome !
}
} Olivier

With my experience I think it is rather normal than surprising that you can
achive relatively good elemental maps in ESI mode but see no recognizable
features in the EELS spectrum.
With an elemental map you get energy loss information for each single image
point. If there is a higher concentration of a certain element, the
intensity of the corresponding pixel in the energy window on the edge will
be higher than in neighbouring pixels not containing this element or only
in less quantity. This does not necessarily mean that you would actually
see a peak in the corresponding spectrum of this pixel, it can be just a
change in the slope of the background. Since you are comparing the changes
in intensity of every pixel this small differences become visible in an
map. Furthermore, in spot mode or selecting a small area with an aperture,
you will average usually over a larger area than a pixel in image mode. If
the features of your specimen are smaller the jump ratio of the edge will
be reduced due to the contribution of the surrounding material.
If you thoroughly compare spectra acquired on a (large enough) area
containing the element of interest and a spectrum of the matrix you will
find differences.
Furthermore you will see certainly more of the edges in your spectra of
thick specimens if you go for a deconvolution. It is still surprising (at
least for me) how much information you can gain from a spectrum which did
not look much more than a steady decrease in intensity.

Petra

--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Thu Mar 30 07:28:45 2000



From: Anne Heller :      heller-at-Uni-Hohenheim.DE
Date: Thu, 30 Mar 2000 10:57:55 +0200
Subject: Re: SEM and TEM of spores

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Corazon Bucana schrieb:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} I would appreciate it very much if someone can refer me to a good book or
} protocol for preparation of fungal spores for SEM and TEM. This is very
} foreign to me since I have been working with mammalian tissues exclusively
} for so many years but now that the lab is a core facility I am getting somel
} requests for other types of samples. Thank you,
}
} Cora Bucana
} *******************************************************
} Corazon D. Bucana, Ph.D.
} Department of Cancer Biology
} U.T. M.D. Anderson Cancer Center
} 1515 Holcombe Blvd. Box 173
} Houston, Texas 77030
} Phone: (713) 792-8106
} FAX: (713) 792-8747
} Email:bucana-at-audumla.mdacc.tmc.edu
} FAX: (713) 792-8747

Dear Cora Bucana,

it is depending very much on the type of spore, e.g. mildew with high water
content and "thin" walls is easy to prepare with standard protokolls for plant
material. Dormant, thick walled spores with less water and high lipid content
are quite tricky to prepare for TEM, but easy for SEM. I can send you a detailed
protocol if you tell more about your fungal spores.

--
Dr. Anne Heller, AG Elektronenmikroskopie,
Institut fŸr Botanik (210), UniversitŠt Hohenheim,
Garbenstra§e 30
D-70593 Stuttgart

Tel.: (0049)-711-459-2180
Fax.: (0049)-711-459-3355




From daemon Thu Mar 30 07:28:47 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Thu, 30 Mar 2000 11:39:50 +0200
Subject: Re: CPD safety question

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On 28 Mar 00, at 10:09, Garber, Charles A. wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Christine Richardson wrote:
} ===================================================
} Hi,
} Our safety people are concerned about having our Polaron c.p.d
} pressure tested at regular intervals.
} Does any one out there know of anyone who does this sort of thing,
} preferably on site? Also I would be interested to hear how other users
} safety check this sort of equipment.
} =================================================== I might not be the
} last word on this, but I had described to me the kind of large liquid
} filled tank (I think it is water) that pressure vessels like this are
} tested in, and to me at least it did not sound like it would be the
} kind of thing that one would "carry around" in a portable kind of unit
} to do this kind of testing, on site. This description came, some
} years ago, from Dr. Wilf Gee (now deceased) and who was very much
} involved in the original manufacturing of this product and who had
} involvement for many years with the safety testing of the unit.
}
} When your CPD unit was first delivered, it would have come with a copy
} of a report from the testing agency showing the results and conditions
} of the pressure testing. I might be off by a bit on this, but it was
} my recollection that the vessel is tested to a pressure level that is
} three or four times higher than what would be required to blow out the
} rupture disc. In other words the unit is tested to a level that, if
} the rupture disc did not blow exactly where it was supposed to blow,
} there is some very large margin of error so that the disc still would
} blow long before the vessel.
}
} There are probably more CPD units of this design installed in the
} world than any other design. I have never heard of anyone having any
} kind of a problem with it (e.g. an explosion), except an occasional
} blowing of a rupture disc, but certainly not the pressure vessel
} itself. Just don't ever try to run the unit by replacing the rupture
} disc, if one is not handy, with a cut metal disc. That is very
} dangerous and should never be done. Believe it or not, we do uncover
} once in a while, people who do do that sort of thing, out of naivety
} of course and that is why I do not waste an opportunity to point out
} the danger in doing that sort of thing. That is why I always have
} recommended, for multi-user environments in particular, to always have
} a small supply of replacement rupture discs near by the unit just to
} avoid even the slightest temptation (such as by a student) to by-pass
} this important safety feature.
}
} So I for one would be very interested in hearing the logic and
} rationale of your safety committee that is of the opinion that you
} should have your pressure vessel tested from time to time.
}
} However, there is one kind of situation where one should have the
} vessel pressure tested and that is if one has attempted any mechanical
} modifications to the chamber itself. Then the system should be
} pressure tested. And since the manufacturer of this particular CPD
} unit (e.g. Polaron) has had on the order of thirty years of
} experience doing this kind of testing, on this particular design of
} vessel, I would recommend having them do the testing for you in the
} UK. Today the testing of the Polaron unit is done to the CE
} standard, without doubt the most stringent in the world.
}
} If you do not have the original test certificate, if you send me your
} S/N I could try to get a copy of it for you. That alone might satisfy
} the questions being asked of your safety people.
}
} Disclaimer: SPI Supplies has offered this particular CPD unit,
} especially to customers outside the USA, for nearly twenty five years
} and we are unaware of any requirement to have the vessel retested at
} periodic intervals for safety reasons.
}
} Chuck
}
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
}
}
}



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Thu Mar 30 07:28:48 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Thu, 30 Mar 2000 11:53:57 +0200
Subject: Re: CPD safety question

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Hello Chuck, and others interested

Firstly, apologies for the earlier reply that was sent before I gave
my comments!

} There are probably more CPD units of this design installed in the
} world than any other design. I have never heard of anyone having any
} kind of a problem with it

We have used two of these units for more than 25 years and the
only problems we have experienced, other than seals which have
had to be replaced from time to time, have been one ruptured disc
and a leak which developed in the supply pipe from the CO2
cylinder. The latter was repaired (new one made up using existing
connectors fitted to new high pressure piping) by our local
compressed gas supplier.

Many years ago, having been told that pressure chambers should
be checked from time to time, I sent one of our units back to be
tested by Polaron in the UK. It came back with a certificate issued
by Probe Technical Services saying that it had been tested to 2500
psi with the comment " Proof pressure applied for one minute
without any visual sign of leakage or failure".

Regards

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Thu Mar 30 07:28:49 2000



From: chris smith :      chris.smith-at-bbsrc.ac.uk
Date: Thu, 30 Mar 2000 12:09:37 +0100
Subject: CPD safety

Contents Retrieved from Microscopy Listserver Archives
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Hi Christine,
We have a Polaron E300 but no demand for routine testing from our 'safety
people' presumably because they know that the burst disc will go long before
the body starts to creak. After a major re-fit it was sent to testing
specialists who gave it a hydrostatic test to 2500 psi for one minute for a
safe working pressure of 2000 psi. It must be possible to do this on site, I
cant imagine big autoclaves or air compressor reservoirs being posted off
for their tests.
It is not as exciting as it sounds. Holes are blanked off and then you pump
it full of water so faults are revealed as leaks rather than the more
entertaining earsplitting bang and shower of shrapnel which could result
from the use of a compressible medium.
If the safety people twist your arm try the Yellow Pages under Engineers,
Inspection & Testing.
ttfn, chris.smith-at-bbsrc.ac.uk
Plant Path Dept., IACR-Rothamsted, Harpenden, Herts. UK.



From daemon Thu Mar 30 07:28:51 2000



From: Brian Robertson :      brian.robertson-at-materials.oxford.ac.uk
Date: Thu, 30 Mar 2000 13:29:07 +0100
Subject: Re: Critical point drier, window cracking

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Dear Gunnar
It is most likely that there was at least one surface scratch
or microcrack that was loaded in a way that resulted in major crack
growth, whose result you observed. During glass removal and
replacement, there were probably several actions that could have
contributed to this -- scratching during cleaning or tool use, altered
clamping of the glass, or turning a scratch on one side of the glass
from inside to outside (or the reverse). Did anyone clamp the glass in
a different way on replacing it, compared with before? (so that the
applied stresses are less uniform or at least different, from before?)
Moisture is well-known (scientifically and by all who specialize in
cutting glass) to enhance crack growth when some component stress
applied to the crack tip region helps to open the crack. All these
could contribute to the failure. The timing of the failure indicates
that one or more of these factors most likely resulted in cracking.
Best wishes for avoiding the problem for another 20 years!
Brian Robertson

------------------
Brian Robertson
Assoc. Prof., Mechanical Engineering, University of Nebraska-Lincoln,
on sabbatical at
Department of Materials, University of Oxford,
Parks Road, Oxford OX1 3PH, United Kingdom
FAX 44+ 1865 273794
brian.robertson-at-materials.ox.ac.uk

* This e-mail message was sent with Execmail V5.0.x *



From daemon Thu Mar 30 17:54:42 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Thu, 30 Mar 2000 14:44:59 +0100
Subject: TEM:EFTEM of particles

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Olivier,
I find it a bit bizarre that you cannot see our mapped features in your
EELS. You're not mapping multiply scattered plasmons are you (a problem
with lower lying edges)? How are you acquiring the EEL spectrum:
Diffraction pattern (spot/CBED)? Or using the image? What sort of count
rates are you getting in the pre-edge images and the maps? Are you sure it
is not noise?

For EFTEM practice, I would if possible, try getting hold of some Al or Mg
particles/powder (not smoke cubes), preferably mixed, and play with imaging
the various plasmons i.e. see which energy windows are best and if possible
see if you can image the oxide layers. It's fun because the signal is
pretty high so easy to see.

Going to core-loss EFTEM the main problems are resolution: For low loss
edges its spatial resolution (mainly delocalization) although there is
evidence that this is not as restrictive as it looks on paper (see Z.L.Wang
Ultramic Vol 67 (1997) pp105-111 for a demonstration in Al).

For higher edges the signal resolution (object resolution function as
Berger & Kohl call it) tells you how good the statistics in a map are. A
good paper is A.Berger & H.Kohl (Optik Vol:92 (1993), No4 pp175-193) which
tells you about both spatial and signal resolution. The equations are quite
rigorous, but it will show you how to get started measuring the
signal-to-noise ratio (SNR). With a bit of practice you can then produce
some SNR plots like those in Hofer, Grogger, Kothleitner & Warbichler
(Ultramicroscopy 67 (1997)pp83-103) which tell you where the energy slit
should be positioned to optimise SNR for a given slit width.

I suppose the best advice is find an easy material to practice with and
work from thereon.
Good luck and have fun.

Jonathan
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Thu Mar 30 17:54:44 2000



From: anderron-at-us.ibm.com
Date: Thu, 30 Mar 2000 09:17:56 -0500
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Alan Fox, in a response to the Analytical TEM string, cautioned the
question-raiser to beware of people who abuse instruments. Perhaps Nestor
will forgive a little microscopy related humor and allow us to start a
string on "Operator Horror Stories." Here's ours:

The "ham-fisted user" reference made us chuckle/cringe with the memory of
a guest "microscopist" in our lab who hauled himself (220 pounds or so) out
of his seat by pulling on the half-inserted specimen rod, bending it about
20 degrees or so!

Henceforth, when we saw him in the hall (he never came into the scope room
again), we referred to him as "Conan the Microscopist"!



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg





From daemon Thu Mar 30 17:54:50 2000



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Thu, 30 Mar 2000 12:11:38 -0500
Subject: FW: TEM, Biological, sinking of tissue in sucrose

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Marie:
You didn't mention the fixative and length of time fixed. Could it be
possible that the tissue is "too well fixed" to allow easy exchange
solutions? Also, have you tried thinner vibratome sections? We routinely
use 40 micron thick brain sections for immuno labeling.
Best regards,
Henry
***********************************************
Marie E. Cantino wrote:
We are preparing perfusion fixed brain tissue by freeze substitution for
post-embedding immuno gold. Following fixation we prepare 500 micron
vibratome sections, which we then wish to infiltrate with 2 M sucrose in
buffer prior to freezing. We have been transferring the brain sections to
1 M sucrose, then when they sink, transferring again to 2 M. The problem
is that the sections never sink in the 2 M sucrose.
*****************************
Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu




From daemon Thu Mar 30 17:54:50 2000



From: Andrew Coutermarsh :      acoutermarsh-at-omegafilters.com
Date: Thu, 30 Mar 2000 12:09:42 -0500
Subject: JOB POSTING

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FOR THOSE WHO MISSED THIS THE FIRST TIME:

JOB POSTING

Company: Omega Optical, Inc.

Location: Brattleboro, Vermont

Company
Description: 30-year old designer and manufacturer of thin film optical
interference filters used world-wide for laboratory and scientific
equipment.

Position: Fluorescence Product Manager

Position
Description: The Fluorescence Product Manager is responsible for any and
all activities which help further the companyâs sales of fluorescence
products. These activities include:

1. Technical sales and sales support: Technical sales and assistance to
sales force regarding fluorescence applications.
2. Marketing: Marketing activities related to fluorescence products.
3. Product & Market Development: Development activities related to new
fluorescence products, applications, and developing markets.

Omega is seeking an individual who is excited about working with varied
applications of fluorescence detection in the life sciences. The work
involves discussing the best match between laboratory experiments and
Omegaâs wide range of light filters for this science. Experience in
fluorescence, microscopy, life sciences and instrumentation is
essential.

Salary: Competitive with full benefit package.

Contact
Information: Email response with attached Word file resume and salary
history to: acoutermarsh-at-omegafilters.com or snailmail response to:


Andrew Coutermarsh, SPHR, HR Director
Omega Optical, Inc.
PO Box 573
Brattleboro, VT 05302


--
================================================================================

"If I am not for myself, who will be? If I am only for myself, what am
I? If not now, when?"
----Hillel




From daemon Thu Mar 30 17:54:52 2000



From: Paula Allan-Wojtas :      ALLANWOJTASP-at-EM.AGR.CA
Date: Thu, 30 Mar 2000 13:02:03 -0500
Subject: LM/EM Embedding Oven

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Hi, All,

I am posting a message for a colleague who does not subscribe to this list.

He is looking for small embedding oven which can polymerize resins in the range of 40¡-70¡C, but which has a very precise temperature control (holds the temperature well, with very little fluctuation during polymerization). He is having problems with the oven he presently has because the temperature is not controlled well enough, and he is getting uneven polymerization.

Another condition is that he is in the middle of a project and needs the oven right away. He had an oven in mind, but it could not be delivered for 6 weeks!!!

Any suggestions from users or vendors are welcome. Please contact me offline, and I'll forward the replies to my colleague.

Thanks again for all your help.

Paula.

Paula Allan-Wojtas
Research Scientist, Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Thu Mar 30 17:54:54 2000



From: C. Singla :      csingla-at-uvic.ca
Date: Thu, 30 Mar 2000 10:18:35 -0800
Subject: Rupture disc for Bomar CPD

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Hallo friends,
I have a Bomar SPC 1500 critical point dryer and I am looking for the
rupture disc size or part number and a vendor. I looked in the Bomar manual
but I can't find any information about it. I shall very much appreciate any
information about it.
Thanking you in advance.
C.Singla



From daemon Thu Mar 30 17:54:55 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Thu, 30 Mar 2000 11:20:35 -0700 (MST)
Subject: Stretch?? Epoxy sections?

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Hi,

I agree with the disagreement that has been raised to my posting of my
personal criteria for good embedment. (A section that stretches in the
boat on exposure to solvent fumes is suboptimal and the embedment needs
changing.)

I have not dealt much with plant material - mycobacterium is the closest I
have come to dealing with cell walls which are tough as Michelin tires. I
also have not used glass knives for more than a decade. Even my students
start out on an old diamond knife. I am no longer familiar with the
vagrancies of glass knives (every one of them seems to have a different
cutting edge! Never twice the same!).

Certainly epoxies belong to the organic chemists. But they also belong to
the material scientists. I have in front of me a thesis done under the
mentoring of Dr. Giammara - A Material Science Evaluation of Epoxy Systems
for TEM Applications. At one time I got into a huge fight with embedding
filters of certain types. I had a computer program which calculated for
me using WPE numbers and molecular weights 36 different formulations. I
actually investigated 25 of them. Totally desperate (and mad) I started
attending the material science symposia on polymers at the MSA meetings.
I found this helpful.

My second favorite book is, HANDBOOK OF EPOXY RESINS. I adore it. (This
has caused my friends to question my sanity and make peculiar remarks)
This book has been of enormous help in my various battles (lots of them
won) with epoxies. Certainly it deals mostly with organic chemistry.

On behalf of the many excellent microscopists I have known for many years
I disagree violently with the statement that "and most EM biologists (AT
LEAST THE GOOD ONES) understand a lot about organic chemistry."
(Capitalizations are mine). One look at this most valuable of books makes
it clear that most of the good EM biologists have no hope of knowing
enough about polymer chemistry to understand or deal with the varied
problems which can occur with epoxies.

Sections in our laboratory do not "stretch". Unless, of course, I want
them to, for other special reasons. Our standard sections are the same
size as the block face they come from. We deal with keratinized skin
which is no picnic compared other biological tissues.

As usual, I always reserve the right to be wrong.

Hildy Crowley
Sr. Electron Microscopist
Dept of Biol Sciences
University of Denver
Denver, CO

P.S. My book is under lock and key at all times. If it is stolen, I will
have to be hospitalized!



From daemon Thu Mar 30 17:54:55 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 30 Mar 2000 10:23:23 -0600
Subject: Re: Leaf Microlumina

Contents Retrieved from Microscopy Listserver Archives
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At 08:42 PM 3/29/00 , I wrote:

} This is on ebay now. Here is the URL:
}
} http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=296173307
}
} Not announced on ebay, and limited to MSA, I am offering
} an IBM Thinkpad 355 which is optionally available to make this camera truly
} portable. $350 extra gets the Thinkpad. You will still need a Slim SCSI
} PCMCIA card, but these are very low cost. I have one in a Sony VAIO
} and it works great with the Leaf.

It is on ebay and "Now announced on ebay, ..." It is NOT limited to MSA.
Sorry about the poor eye-hand coordination.

gary



From daemon Thu Mar 30 18:21:48 2000



From: mary mckee :      mckee-at-helix.mgh.harvard.edu
Date: Thu, 30 Mar 2000 17:56:15 -0600
Subject: low-temp polymerization of LR white

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Hello, listmembers,

Has anyone got a protocol for embedding LR white in a low-temperature
chamber using UV light? I have a Leica AFS, so setting and maintaining a
temp. is not a problem. I just tried it overnight at -20 C with UV (in
filled gelatin capsules, but without activator) and did not get
polymerization. Any tips would be appreciated.

Mary McKee
Renal Unit
MGH
Charlestown, MA
(617)726-5643




From daemon Thu Mar 30 18:21:48 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Thu, 30 Mar 2000 17:56:01 -0600
Subject: pressure systems

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Dear UK CPD users,
In the UK pressure systems which operate at pressures
greater than 0.5 bar are subject to the Pressure Systems
and Transportable Gas Container Regulations 1989. These
rules require, among other things, regular inspection of
the apparatus. You may also find that your institution's
insurers impose a similar rule.

Regards,
Eric

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk




From daemon Fri Mar 31 08:11:51 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:03:50 -0600
Subject: GIF: angles calibration

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Originally Posted by: shens-at-focus.ruca.ua.ac.be



Hi,

How we can experimentally measure the collection angle when
working with GIF? In other words, how to measure magnification
between the plane of negatives and the plane of GIF entrance? Using
a CCD camera? But normally a CCD camera magnifies an image 20
times of that on a negative, so pictures become uncomparible.

Kind Regards,

Steven




From daemon Fri Mar 31 08:11:51 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:42 -0600
Subject: M&M2000: Social Event Information

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Originally posted by: Beverly_E_Maleeff-at-sbphrd.com



The Microscopy & Microanalysis 2000 Local Arrangements Committee
is pleased to announce an additional social event at M&M2000---

TAKE ME OUT TO THE BALL GAME !!

Join us on Tuesday, August 15th as the
PHILADELPHIA PHILLIES meet the
ARIZONA DIAMONDBACKS at Veterans Stadium.
Game time is 7:35 PM.

For $32.50, you'll get:
- a ticket to the game (300 level, on the third base line)
- a $10.00 coupon, good toward purchases at all concession and souvenir stands
- round-trip transportation from the Convention Center to the stadium
on a luxury coach (buses leave at 6:00 PM; return to the convention
center immediately following the game)

A limited number of packages are available. First come, first served.
Maximum 2 packages per person.

Checks, in US$ only, payable to "Microscopy & Microanalysis 2000" can be mailed
to:

Bev Maleeff
Treasurer, M&M2000 LAC
c/o SmithKline Beecham Pharmaceuticals
Mail Code UE 0462
709 Swedeland Road
King of Prussia, PA 19406

Checks only, please. No credit cards accepted.
Your cancelled check will serve as your receipt.

Tickets will be distributed at the M&M2000 Hospitality Booth
on Monday and Tuesday, August 14th & 15th.

Further information about this and other M&M2000 events can be found on our web
site:
http://www.msa.microscopy.com/~mm2000/

See you in August!!

Bev Maleeff
M&M2000 LAC




From daemon Fri Mar 31 08:11:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:00:33 -0600
Subject: Job opportunities in Dept of Materials, Oxford, UK

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Originally posted by: ron.doole-at-materials.ox.ac.uk


Hi All,

I enclose details of post doctoral positions currently
available at Dept. of Materials here in Oxford. Please
respond directly to the address given for each post.
Good luck to any applicants,

Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

**********************************************************************
UNIVERSITY OF OXFORD

Department of Materials

POSTDOCTORAL RESEARCH POSITIONS - Salary range £16,286-£24,479p.a.

Applications are invited for the following positions:

Manufacture of Metal Matrix Composite Components

A position is available for a period of 20 months for a suitably qualified
research assistant to join an inter-disciplinary research team in the
Department of Materials and Department of Engineering Science researching
into the manufacture of next generation aeroengine components from long
fibre reinforced metal matrix composites. This post will study the
fundamentals of fabrication mechanisms, and how these influence subsequent
mechanical performance, using a combination of numerical modelling and
experiments. Candidates should preferably have expertise and ability in one
or more of the following areas: experimental solid mechanics, finite
element analysis of plasticity or metal forming, microstructural
characterisation of materials, composite materials. Please quote DJ00/6

Interface Modification in Organic LED

A position, sponsored by Opsys Limited, is available in the Department of
Materials from April 2000 (or as soon as possible thereafter) for 18 months
in the first instance. The project will explore routes to modifying the
interfaces of the OLEDs with the objective to improve charge transport and
injection. It will involve detailed investigation of the structural and
physical properties of the modified interfaces between organo-lanthanide
phosphors and other organic and inorganic materials. Techniques will
include UPS, SIMS, AFM/STM and electro-optical characterisation of OLEDs.
Expertise in one or more of these is highly desirable. Further details are
available by email from: oleg.salata-at-materials.ox.ac.uk. Please quote
DJ00/7.

Compositional inhomogeneities in information storage materials - effect on
physical properties
(Principal investigators: Amanda Petford-Long and Alfred Cerezo)

A 3-year position funded by the EPSRC, with support from Seagate
Technology, is available in the Department of Materials from May 2000 (or
as soon as possible thereafter). The aim of this project is to address how
local inhomogeneities in the morphology and composition of thin layered
films used for spin-valves and media in information storage technology are
controlled by processing of the materials, and how this in turn determines
their magnetic properties. The project will primarily involve the use of
three-dimensional atom probe (3DAP) analysis to study the chemistry of the
films at near-atomic resolution. Results from 3DAP analysis will be
combined with measurements of magnetic properties to elucidate the role of
the observed nanostructural features on the physical film properties. Some
electron microscopy will also be required for comparative microstructural
analysis. Expertise in materials characterisation is essential, and some
knowledge of thin layered films or magnetic materials would also be
helpful. Please quote ref. DJ00/8.

Applications including a full CV, list of publications and the names and
addresses of three referees should be sent to The Administrator, Department
of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, from whom
further particulars are available. The closing date for applications is 20
April 2000.




From daemon Fri Mar 31 08:11:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:01:46 -0600
Subject: Operator Horror Stories

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Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri Mar 31 08:11:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:02:50 -0600
Subject: CPD testing

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Originally posted by: cgarber-at-2spi.com



-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

As further comment on the pressure testing of the Polaron ® CPD units, I
have been asked to submit the following statement from David Cheshire, a
Manufacturing Manager at VG Microtech, the manufacturers of the Polaron
brand of critical point dryers:
=================================================
We use an independent test facility that has amongst its credentials the
following international approvals : NAMAS, UKAS, NATA. The test
certificates issued by ERA are for each individual chamber and we have
copies of all these. The test itself is a sustained 50% overpressure (2000
psi) for 1 minute. Obviously any leakage would lead to failure and non
certification. In the past units have been overpressured to 2500 and 3000
psi.

I think it is highly unlikely that a "local dive shop" would have the
facilities and wherewithal to support the standards imposed by the various
associations.

Incidentally the window is not quartz but a far denser and tougher material
specifically manufactured for high pressure use.

Best regards
Dave Cheshire

Polaron Range Development Manager
VG Microtech
The Birches Industrial Estate,
Imberhorne Lane,
East Grinstead,
West Sussex.
RH19 1UB.
UK.
===================================================
I believe there was some concern about having this kind of testing, if it
was to be done, in any kind of facility that was not certified according to
the bodies mentioned in David's message.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Mar 31 08:11:53 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:09 -0600
Subject: Administrivia: Oopps.. Nestor broke things

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Colleagues....

In trying to adapt the SPAM filter last night to catch the latest
porn site posting I accidently broke the software. Most messages
got rejected after ~ 5 pm Chicago time. I will try to repost
those messages , however, I may have missed a few. If you
dont' see your posting by the end of the day, please resend it
and accept my apology.

Nestor
Your Friendly Neighborhood SysOp




From daemon Fri Mar 31 08:11:53 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:04:30 -0600
Subject: SEM - Coating

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Originally posted by: bart.depauw-at-rug.ac.be


Hello,

I would like to know why they use Argon in the metal coating devices. At
our laboratory, we don't use Argon, we use air. What is the advantage of
using Argon ?
De Pauw Bart
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90




From daemon Fri Mar 31 08:11:53 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:00:59 -0600
Subject: Ni grids with holey carbon film

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Originally posted by: willem.erasmus-at-sasol.com



Dear fellow microscopists

Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.

Thanks
W. Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 9603954
Fax : +27 +16 9602826
E-mail : willem.erasmus-at-sasol.com




From daemon Fri Mar 31 08:23:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:21 -0600
Subject: LM: Help AO Microscope

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Originally Posted by: picomagic-at-canada.com






Email: pink-at-memphis.magibox.net

Name: Bill Cupo

Question: Many years ago the museum bought an AO microscope with an
Expostar Shutter control.
It appears that the control box (model 1190) has been lost.
Is there anyway to buy a replacement?
Does the company still exist? Is the microscope manufactored under another
name?

Any assistance will be greatly appreciated.

Bill Cupo
Exhibits Media Specialist

---------------------------------------------------------------------------




From daemon Fri Mar 31 08:23:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:59 -0600
Subject: XIth International Congress of Histochemistry and Cytochemistry

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Originally Posted by: george.bou-gharios-at-csc.mrc.ac.uk

I am writing to you in my capacity as secretary general of the XIth
International Congress of Histochemistry and Cytochemistry which is held in
YORK, UK 3-8 July 2000.
The above mentioned Congress is Hosted by the Royal Microscopical Society
(www.rms.org.uk) and n exciting programme has been put together which can
be seen on

www.med.ic.ac.uk/external/ichc_2000/


Dr George Bou-Gharios
Muscle Cell Biology Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital
Du Cane Road
London W12 0NN
Tel: 0181-383 8261
Fax: 0181- 383 8264
email: george.bou-gharios-at-csc.mrc.ac.uk




From daemon Fri Mar 31 08:23:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:14:35 -0600
Subject: IUMAS 2000 Program

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Originally posted by: jfmjfm-at-engin.umich.edu


Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"




From daemon Fri Mar 31 18:08:43 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:24:27 -0600
Subject: IUMAS 2000 Program

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Originally posted by: jfmjfm-at-engin.umich.edu



Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"




From daemon Fri Mar 31 18:08:47 2000



From: Christoph Benning :      benning-at-pilot.msu.edu
Date: Fri, 31 Mar 2000 09:46:29 -0500
Subject: TEM-Plant Root Plastids

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Hi there:

I would like to know whether the ratio of plastidic to extraplastidic
membranes changes in roots of Arabidopsis or any other plant in response to
phosphate starvation. Does anyone know of a TEM ultrastructural
morphometric analysis or any other type of analysis towards this end?

Thanks, Christoph Benning

Christoph Benning

Assistant Professor
Dept. of Biochemistry
Michigan State University
East Lansing, MI 48824-1319
USA

Phone: (517) 355-1609
Fax: (517)-353-9334
http://www.bch.msu.edu



From daemon Fri Mar 31 18:08:47 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 31 Mar 2000 09:57:35 -0400
Subject: RE: Ni grids

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The problem with Ni grids is that Ni is a magnetic metal, and so Ni grids
can give all kinds of trouble. We used to use them back in the early days
when they were about all that was available, and when resolution of EMs
wasn't so great; now-a-days they have lost their popularity.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 31 18:08:49 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:02:39 -0800
Subject: Need a non-fluorescing plastic slide

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Hi,

This is a posting for a colleague.

He needs a source of plastic slides and/or film which can be machined and
which do not fluoresce. Anybody have any ideas? Contact me offline.

Many thanks
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Fri Mar 31 18:08:49 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:10:32 -0800
Subject: Re: LM - mirror objectives available?

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From daemon Fri Mar 31 18:08:49 2000



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 31 Mar 2000 10:11:18 -0600
Subject: RE: nickel grids

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} ----------
} From: Springett, Margaret J.
} Sent: Friday, March 31, 2000 11:04 AM
} To: 'springett.margaret-at-mayo.edu'
} Subject: nickel grids
}
} I do an extensive amount of immunolabeling, and nickel grids are
} economical,
} but two factors need to be considered on a daily basis. The fact that
} they
} are magnetic can be an advantage as well as a detriment. Their magnetic
} quality allows one to rinse grids floating on a bubble with a magnetic
} stir
} plate, this has been demonstrated in Dr. Bendyan's workshops on
} goldlabeling
} techniques. When trying to retreive a stubborn grid out of a grid box, a
} magnetic tweezer is "heaven sent". Now about magnetic grids in the scope,
} if you stigmate the scope after fine-focusing, your micrograph will be
} focused. I have used these techniques to produce excellent micrographs of
} caveolae at better than 100K magnification. For our use gold grids are
} too
} expensive, so "work around methods" with nickel grids are necessary.
} Marge
}
}
Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation and Clinic
Rochester, MN 55905





From daemon Fri Mar 31 18:08:49 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:10:32 -0800
Subject: Re: LM - mirror objectives available?

Contents Retrieved from Microscopy Listserver Archives
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Frank,

There is an entire FTIR imaging industry out there. SpectraTech has been a
leader in that industry and is a good place to start. Suggest you call Ed
Manke (203-926-8998). Also, John Reffner has been one of the few people
who has really bridged microscopy and spectroscopy. He is the real guru in
this area. John is currently working with SensIR technologies and can be
reached there at 203-207-9700.

The other companies who are in this area come from more of a spectroscopy
background (Jobin Yvon/Instruments SA, Perkin Elmer, Hewlitt Packard, etc.)
and would know less about the microscopy interface.

I taught a microscopy course to the apps specialists at Spectra Tech this
January and had a chance to work with their Continuum, which has a
combination of both glass and reflecting optics on its nosepiece. However,
I think that the highest magnifications they had availalable were only
about 32x objectives. The NAs were really great as I remember, so you
could make use of electronics from the CCD to boost the actual mag to the
screen.

Let me know how you make out.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************

At 08:12 AM 3/31/00 -0600, Nestor J. Zaluzec wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 31 18:08:50 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:14:16 -0800
Subject: Re: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
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From daemon Fri Mar 31 18:08:50 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:14:16 -0800
Subject: Re: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
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Bill,

AO became part of Cambridge in 1986 then came under the Leica umbrella in
1990. I'd suggest that you start with Leica in Deerfield, IL. Wayne
Buttermore is still there from the "old days" and may have a suggestion:
847-405-7044. The alternative would be to ask around in the used equipment
market. Here are some of my contacts:
MicroOptical Methods Dennis O'Leary Albany, NY 518-482-8200
Spectra Services Mike Specht Rochester, NY 716-654-9500
Vermont Optechs John Oren 802-425-2040
Martin Instruments Bob Martin Easley, SC 864-859-2688

Good luck.
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************




At 08:13 AM 3/31/00 -0600, Nestor J. Zaluzec wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 31 18:08:51 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 31 Mar 2000 09:20:25 -0700
Subject: Operator Horror Stories

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We once had a student twist the condenser control knob off a TEM. When asked
to turn the knob clockwise, he just kept turning, until he handed me the
knob.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Friday, March 31, 2000 7:02 AM
To: Microscopy-at-sparc5.microscopy.com



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri Mar 31 18:08:51 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 31 Mar 2000 11:23:36 -0500 (Eastern Standard Time)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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I had one faculty operator several years ago who after a SINGLE lesson
on the Phillips 200, decided to come back late at night and look some
more. After taking some images, he couldn't get the door to the camera
chamber off, since he had failed to vent it. After rummaging through
the lab, he found a large screwdriver and commenced to pry the camera
door off, unconcerned about the marks and gouges that he was putting in
the brass. Needless to say, when the vacuum was finally broken, the
mercury diffusion pump became very unhappy. Eventually we were able to
replace the instrument and the user finally left after being denied
tenure.

W. L. Steffens, Ph.D
Dept. of Pathology
University of Georgia



From daemon Fri Mar 31 18:08:52 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 11:43:34 -0500
Subject: Re: Ni grids with holey carbon film

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We get our Ni/holey carbon films from SPI, at http://www.2spi.com/.
They will prepare excellent holey carbon films on just about any
available grid material (for a price of course...).

Larry





} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Fri Mar 31 18:08:53 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 31 Mar 2000 12:27:46 -0500
Subject: Re: Ni grids with holey carbon film

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Nestor J. Zaluzec wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Originally posted by: willem.erasmus-at-sasol.com
}
} Dear fellow microscopists
}
} Does anybody know where I can purchase Ni TEM grids with a holey carbon film
} ? I can find plenty of copper grids, but the Ni grids seem to be hard to
} find.
}
} Thanks
} W. Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 9603954
} Fax : +27 +16 9602826
} E-mail : willem.erasmus-at-sasol.com

We at Ladd Reseach can supply you with these, as would most of the other
supply houses that make their own holey film. Please let us know what
size mesh you want and a quantity and we can quote you.

Debbie Sicard
Sales Manager

Disclaimer: Ladd Research is a vendor that sells EM supplies.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Fri Mar 31 18:08:53 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 12:08:51 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The respected PhD I once worked with on our old JEOL JSM-U3 scanner,
who was having trouble making out the details of the image on the
monitor, so grabbed a flashlight and shined it at the screen so he
could see the image a little better... :-).

Larry

}
}
} Originally posted by: sryazant-at-ucla.edu
}
}
}
}
} It is not "horror" story, but a sort of... Many years ago the colleague
} from friendly Lab visited me with great project. The idea was simply and
} beautiful. She argues that because the image in the scope (TEM) is green
} (green fluorescence of the screen), she wants to modify the sample (I
} forgot what, some protein, I believe) by red-fluorescent dye to be able to
} see on the screen of the electron microscope the "double-staining": red on
} the green background. No comments...
}
} Sergey
}
} } Date: Thu, 30 Mar 2000 09:17:56 -0500
} } From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} } Subject: Operator Horror Stories
} } To: microscopy-at-sparc5.microscopy.com
} } Importance: Normal
} } X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
} (Intl)|18
} } January 2000) at 30/03/2000 09:18:01
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Alan Fox, in a response to the Analytical TEM string, cautioned the
} } question-raiser to beware of people who abuse instruments. Perhaps Nestor
} } will forgive a little microscopy related humor and allow us to start a
} } string on "Operator Horror Stories." Here's ours:
} }
} } The "ham-fisted user" reference made us chuckle/cringe with the memory of
} } a guest "microscopist" in our lab who hauled himself (220 pounds or so) out
} } of his seat by pulling on the half-inserted specimen rod, bending it about
} } 20 degrees or so!
} }
} } Henceforth, when we saw him in the hall (he never came into the scope room
} } again), we referred to him as "Conan the Microscopist"!
} }
} }
} }
} } Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
} }
} } IBM Analytical Services; http://www.chips.ibm.com/services/asg
} }
} }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Fri Mar 31 18:08:54 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Mar 2000 13:39:47 -0500
Subject: Carbon coated Ni grids

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Erasmus asked:
================================================
Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.
=================================================
Some times the coating of Ni grids is a bit more difficult than the coating
of copper grids. This translates to somewhat lower yields and sometimes
higher prices. However, SPI Supplies regularly produces custom coated Ni
grids on whatever mesh and grid the customer specifies and at only a slight
price premium over copper.

Contact me off line for details or visit our webpage URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Some of the other major suppliers of consumables also will coat Ni grids, or
at least that is my understanding.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Mar 31 18:08:54 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 Mar 2000 08:45:16 -1000 (HST)
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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One day a user of our TEM complained that she could see the beam with the
specimen out, but as soon as she put in a grid the screen went
black. After playing with it awhile and ruling out magnetism or something
on the grid holder, I decided that there must be some small magnetic
particle in the stage area that was being pushed around by the grid
holder. Of course the service technician I got by phone wanted me to
begin taking the column apart from the gun on down, but I grew impatient
with this and soon went straight for the stage. I figured I would be
looking for some small metal sliver. Imagine my surprise when I found an
entire plastic pipettortip lying across the bore through the
anticontamination plate in the column! It seems the previous user had
been using these tips as forceps covers, and mysteriously lost one the day
before. It had gone into the column through the airlock with her grid,
not impossible with the Zeiss 10 top-loading system. Although not the
only strange thing I have found in that microscope's column, at about an
inch and a half long it is the largest.

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Fri Mar 31 18:08:55 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 31 Mar 2000 12:34:21 -0600
Subject: TEM/peroxidase immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently using the peroxidase/DAB pre-embedding method for
localization of a novel extracellular matrix protein. I am using 40 micron
vibratome sections. While my results give some extracellular labelling,
most of the labelling is associated with dendritic microtubules. Has anyone
experienced non-specific labelling of microtubules?



From daemon Fri Mar 31 18:08:56 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 31 Mar 2000 14:48:58 -0500 (EST)
Subject: Core Facility solvency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscpy Core Managers,
Are there any EM/Confocal Microscope facilities within academia that
are running in the black or at least breaking even without a subsidy. If
so what are your rates and overhead, are your salaries included? Also are
there any companies or facilities that do tele-microscopy? All info will be
shared with the listserver, unless confidentiality is requested. Thank You.

Michael Delannoy
Basic Science Microscopy Facility
JHMI



From daemon Fri Mar 31 18:08:58 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 31 Mar 2000 15:46:00 -0600
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Fri Mar 31 18:09:00 2000



From: L R MELSEN :      lmelsen-at-emory.edu
Date: Fri, 31 Mar 2000 16:19:48 -0500
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We had a cousin of Conan , who in his addled age, after inserting the
injector tip into the stage of our EM400 could not find his grid in the
microscope; he had dropped it on the floor. Of course the tip must have
fallen off in the stage, so he promptly took a second tip and properly
inserted the second tip on top of the first. Still no grid could be
found. Ah ha, I will leave a polite note explaining the problem. It read
as follows:
" Please check the microscope, I had great difficulty inserting my
specimens last evening."
Philips kindly replaced the bulk of the stage just so they could keep
the original for the museum of what not to do.



From daemon Fri Mar 31 18:09:01 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 31 Mar 2000 16:26:02 -0500
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Two from the University of Florida.

There once were two eager graduate students at Florida in the Materials
Science and Engineering Department (one has the initials MK and now works
for JEOL) that decided that they wanted learn how to align the Philips 300
TEM. Armed with the user manual, they proceeded to align the column, but
couldn't do it. They had to call in the service engineer. The first thing
that the service engineer did was to use a framing square to help to take
the visible bow out of the column. It seems that the user manual they were
working from was different from one for a microscope with a STEM attachment.
(Not witnessed by me, but heard from reliable sources.)

A professor in the same department was escorting a visitor around the EM lab
and the two were discussing possible experiments that might be performed in
the Philips 300. They asked the EM technician to give them a hand opening
up the microscope so they could look inside. The technician was busy and
said that he would be with them in about 5 minutes. Well, busy professors
can't wait for lowly technicians so they decided to open the chamber
themselves. The professor cranked on the handle to open the gun chamber
while it was under vacuum and the voltage was on. Bang, pop, whoosh,
whistles, bells brought the technician running to see what had happened.
After all, they only wanted to look inside. But, there was a happy ending
to the story. The vacuum and diffusion pump were OK and the apertures were
cleaned by the supersonic air. (I was around when this one happened.)

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} [mailto:"anderron-at-us.ibm.com"-at-sparc5.microscopy.com]
} Sent: Thursday, March 30, 2000 9:18 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} Alan Fox, in a response to the Analytical TEM string, cautioned the
} question-raiser to beware of people who abuse instruments.
} Perhaps Nestor
} will forgive a little microscopy related humor and allow us to start a
} string on "Operator Horror Stories." Here's ours:
}
} The "ham-fisted user" reference made us chuckle/cringe with
} the memory of
} a guest "microscopist" in our lab who hauled himself (220
} pounds or so) out
} of his seat by pulling on the half-inserted specimen rod,
} bending it about
} 20 degrees or so!
}
} Henceforth, when we saw him in the hall (he never came into
} the scope room
} again), we referred to him as "Conan the Microscopist"!
}
}
}
} Ron Anderson, IBM, Hopewell Jct., New York, USA.
} anderron-at-us.ibm.com
}
} IBM Analytical Services; http://www.chips.ibm.com/services/asg
}
}
}
}



From daemon Fri Mar 31 18:09:01 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Fri, 31 Mar 2000 16:48:24 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my second go around with this, I got the Nestor porno bump this
morning.

I had a student using my JEOL 100 CX II; loading and looking around went
well. When she went to remove the holder she got half way out and yanked it
toward her. This action put a 70 degree bend in the holder. I could never
get the Z height to behave after that for some odd reason.

The other disaster was with my Philips 300, complete with Mercury Dif pump
at that time. I needed to do some anode cleaning, so without deflating the
air table I got on the console finished my work and got off. When the scope
lurched to the right it dumped the contents of the mercury pump into the oil
pump. This sent a cloud of mercury up the column creating an alloy wherever
it went.

Believe it or not the scope is in fine working order with not a trace of
mercury left in in the column. How did I do it? If you can figure it out I
will send the winner a Lucent/Bell Labs brief case...at my cost of course.

John Grazul
Lucent Technologies

"W. L. Steffens" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I had one faculty operator several years ago who after a SINGLE lesson
} on the Phillips 200, decided to come back late at night and look some
} more. After taking some images, he couldn't get the door to the camera
} chamber off, since he had failed to vent it. After rummaging through
} the lab, he found a large screwdriver and commenced to pry the camera
} door off, unconcerned about the marks and gouges that he was putting in
} the brass. Needless to say, when the vacuum was finally broken, the
} mercury diffusion pump became very unhappy. Eventually we were able to
} replace the instrument and the user finally left after being denied
} tenure.
}
} W. L. Steffens, Ph.D
} Dept. of Pathology
} University of Georgia



From daemon Fri Mar 31 18:09:03 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Fri, 31 Mar 2000 16:03:27 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I got in an agument with an engineering prof that disputed
the inverse square law applied to photo flash units.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri Mar 31 18:09:06 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Fri, 31 Mar 2000 16:40:27 -0600
Subject: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once, over a decade back, I was working EM and Med Tech at the same
time at a local hospital..

We had a CAP inspection, the officers were from another hospital in the state.


The question I was expected to reason and answer with a straight face was:


* What other protective measures besides standing behind a lead
wall do you take when you put the glass slide into the TEM, to
protect yourself from the flying electrons???



seriously!

I was rather stunned, and started looking from the wall socket to
him and back, but he didn't catch the connection.


I then tried to tactfully explain that vacuum was required, and that
we really didn't need that extra protection!

It was explained to me that he did too know all about EM because he
had a one day workshop.



Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } }
Lou Ann Miller
Service Supervisor

Rm 1108 VMBSBld.
College of Veterinary Medicine
Veterinary Biosciences
2001 S. Lincoln Ave
Urbana, IL 61802

Phone: 217-244-1567
Fax: 217-244-1652

lamiller-at-uiuc.edu
lamiller-at-net66.com
turtle_lam-at-yahoo.com

Web Pages:
Lab Page
http://treefrog.cvm.uiuc.edu
Centeral States Microscopy Page
http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page
http://treefrog.cvm.uiuc.edu/lam



From daemon Fri Mar 31 18:09:09 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 31 Mar 2000 17:58:37 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We once had an operator on our JEOL 840A SEM complain about her
} sample moving. Her sample was samples of vacuum grease she had gold
} coated and was attempting to image in the SEM. When the electron
} beam heated the grease it cracked up the gold coating and charged.
} She was using the SEM to look for silicon in various greases. I
} suggested she use a different technique so we did not end up with
} vaporized grease inside the SEM.

David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Fri Mar 31 18:09:12 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 Mar 2000 16:48:19 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems everyone has one of these. I had a person who one day
complained of a burning smell while she was at the scope. I went to
investigate and when I arrived in the room there was no smell... burning
or otherwise. Several more times that session she came and got me to
investigate this burning smell in the TEM room. This continued for a
couple of sessions. I did go and investigate because after all, who
wants their TEM on fire? I honestly didn't know what to do about it and
was beginning to seriously question her sanity. Finally she convinced
me to sit with her while she operated the scope and sure enough there
was this faint smell of something burning! I jumped up and tried to
find it, but it had gone away. So...she sat down again and began to
work and the smell, like plastic kitchenware burning in a dishwasher,
came back. Again I jump up and look around....to no avail! I was
curious now. The burning smell only happens when she is sitting at the
scope. Not being a big believer in spontaneous combustion, there had to
be an answer! There was. As part of the TEM course I take the kick
panel off the Zeiss 10 to show the students the guts of the vacuum
system. The kick panel is right below the desk area where you sit up to
the microscope. What she was doing was placing her tennis shoe on the
heater of the diffusion pump and the plastic/rubber would melt and burn
a bit -- just enough to give the room a burning smell. She never did
notice her foot getting warm. When I pointed it out she did admit that
her shoe was warm and had some ugly scars on it to boot! She never
returned after that semester - I guess that she had enough of EM! Now I
carefully point out the hazard and replace the cover quickly when we are
finished with the class.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Fri Mar 31 18:09:16 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Fri, 31 Mar 2000 16:35:42 -0700
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


And all of these cute blunders that happened way back when were caused by
today's scientists/researchers.

Experience, good and bad, is the only way we all really learn.

Enjoying the stories.....

Harry Ekstrom
Honeywell Materials Laboratory



-----Original Message-----
} From: White, Woody N [mailto:Woody.N.White-at-mcdermott.com]
Sent: Friday, March 31, 2000 2:46 PM
To: Microscopy-at-sparc5.microscopy.com



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Fri Mar 31 18:09:16 2000



From: Amy McGough :      amcgough-at-bcm.tmc.edu
Date: Fri, 31 Mar 2000 17:50:10 -0600
Subject: Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position Announcement

Research Scientist in Macromolecular Microscopy

Purdue University Department of Biological Sciences

An opening is available immediately for a scientist specializing in
macromolecular microscopy at Purdue University. The successful
candidate would be responsible for overseeing the day-to-day
operations of the high-resolution electron microscopy facility, training
new users, and assisting outside visitors with data collection and
analysis. He/she would also be expected to work with the cryoEM
groups in the department at planning and implementing the future
directions of the facility, including the development of new
technologies for improved specimen preservation, data collection, and
analysis. The ideal candidate should complement the existing
strengths represented in the department and pursue independent
research funding.

The Department of Biological Sciences at Purdue provides a rich
intellectual environment as well as outstanding modern scientific
facilities including Philips EM420, CM200-FEG, and CM300-FEG
electron cryo-microscopes. Purdue University is home to over 50,000
students, faculty, and staff, and is located in West Lafayette, Indiana -
approximately 1 hour northwest of Indianapolis and 2.5 hours
southeast of Chicago. The area affords the advantages associated with
a major university, while providing an affordable and attractive `small
town' environment in which to live.

Requirements for this position include a Ph.D. in biophysics or one of
the related sciences, a minimum of 5 years post-doctoral work
experience in electron cryo-microscopy of biological molecules, and a
proven ability to work well with others. Preference will be given to
candidates who have successfully developed new technologies and
methodologies for high-resolution electron cryo-microscopy. Salary
will be commensurate with experience and includes full benefits.

Interested individuals should send their resume, relevant reprints, a
statement of research interests and career goals, and provide contact
information (name/phone/e-mail) for three individuals who have
agreed to serve as references to:

Dr. Timothy S. Baker (tsb-at-bragg.bio.purdue.edu)

and/or

Dr. Amy McGough (amcgough-at-bcm.tmc.edu)

Department of Biological Sciences
Lilly Hall of Life Science
Purdue University
West Lafayette, IN 47907-1392

Purdue is an equal access/equal opportunity university.



From daemon Fri Mar 31 18:09:17 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 31 Mar 00 16:04:07 -0800
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Is there any truth in the story I was told about a lab in London that replaced the leaded glass on the microscope chamber with regular glass? Apparently the mistake was discovered when all the film on the shelf behind the operator become fogged!

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Mar 31 18:19:25 2000



From: Jo Dee :      jofish-at-burnham-inst.org
Date: Fri, 31 Mar 2000 16:04:43 -0800
Subject: Re: Operator Horror Stories

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Message-ID: {38E53D1B.C043653A-at-burnham-inst.org}


Hello All,
My two horror stories!
I was working with an investigator who wanted to see matrix vessicles. Well,
we were so happy that we had a nicely fixed sample with "lots" of vessicles.
Nice whole round ones. Well, when the negatives were developed, my director
and I discovered that what we really had were nicely fixed, beautiful
bacteria!!!! No vessicles!
Another time, in school, I was walking down the hallway later in the afternoon
when I saw a scope room door slightly open. I popped my head in to see who was
there and to ask if they wanted the door closed. When I looked inside, I found
a student sound asleep, with hands still holding the knobs of the
microscope!!!!!!!
Funny huh?
Jo

"anderron-at-us.ibm.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Alan Fox, in a response to the Analytical TEM string, cautioned the
} question-raiser to beware of people who abuse instruments. Perhaps Nestor
} will forgive a little microscopy related humor and allow us to start a
} string on "Operator Horror Stories." Here's ours:
}
} The "ham-fisted user" reference made us chuckle/cringe with the memory of
} a guest "microscopist" in our lab who hauled himself (220 pounds or so) out
} of his seat by pulling on the half-inserted specimen rod, bending it about
} 20 degrees or so!
}
} Henceforth, when we saw him in the hall (he never came into the scope room
} again), we referred to him as "Conan the Microscopist"!
}
} Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
}
} IBM Analytical Services; http://www.chips.ibm.com/services/asg



From daemon Fri Mar 31 18:19:25 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 31 Mar 2000 18:10:58 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
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Bart,
I believe Argon is used because it is a larger molecule and
sputters the metal more efficiently. I also use air with no problem.
Possibly the amount of metal sputtered is slightly less per unit time.
John Hunt

On Fri, 31 Mar 2000, Nestor J. Zaluzec wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
}
} Originally posted by: bart.depauw-at-rug.ac.be
}
}
} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90
}
}
}




From daemon Fri Mar 31 18:19:26 2000



From: pe13-at-cam.ac.uk
Date: Fri, 31 Mar 2000 18:11:27 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Air contains water vapour, oxygen and carbon dioxide all of which can be
broken down in the plasma generated in a sputter coater and gve rise to
nasty active species which can damage delicate samples. Argon is used
because of its relative high atomic number and this ensures there is
multiple scattering of the sputtered metal. There is a Zenon sputter coater
on the market which should sputter even better but I have no experience
with it. Come to the Lehigh Short Course(contact {slc6-at-lehigh.edu) and we
will tell you all about it.

Patrick Echlin
Cambridge




From daemon Fri Mar 31 18:19:26 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Fri, 31 Mar 2000 18:12:12 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Sat Apr 01 07:39:47 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.



From daemon Sat Apr 01 07:39:52 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger



From daemon Sat Apr 01 07:39:53 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 23:10:28 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

I heard that one years ago. Also one that supposedly happened at Univ.
Wisconsin. Someone apparently cleaned a scope's internal parts in acetone
and started pumping. Before it was fully pumped down, they turned on the
filament. Bang! Nice story but could it possibly happen? I would think
that any acetone residue would evaporate very quickly or is there some
other phenomenon here relating to ratio of gases?

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897

} Is there any truth in the story I was told about a lab in London that
} replaced the leaded glass on the microscope chamber with regular
} glass? Apparently the mistake was discovered when all the film on the
} shelf behind the operator become fogged!
}
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} phone:213 273 8026
} fax: 213 413 6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm



From daemon Sat Apr 01 07:40:09 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.




From daemon Sat Apr 01 07:40:13 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger




From daemon Sat Apr 01 07:40:15 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 23:10:28 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

I heard that one years ago. Also one that supposedly happened at Univ.
Wisconsin. Someone apparently cleaned a scope's internal parts in acetone
and started pumping. Before it was fully pumped down, they turned on the
filament. Bang! Nice story but could it possibly happen? I would think
that any acetone residue would evaporate very quickly or is there some
other phenomenon here relating to ratio of gases?

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897

} Is there any truth in the story I was told about a lab in London that
} replaced the leaded glass on the microscope chamber with regular
} glass? Apparently the mistake was discovered when all the film on the
} shelf behind the operator become fogged!
}
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} phone:213 273 8026
} fax: 213 413 6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm




From daemon Sat Apr 01 07:40:20 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, March 31, 2000 9:26 PM
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My story is not as much of a horror as it is embarassing. Several years
back I received a package of samples from a regular client without any paper
work describing what the samples were. This is not unusual since we
frequently recieve unknowns. I proceeded to open the package and was
abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
of the samples was a vial of concentrated bannana flavoring. It was months
(almost a year) before the odor completely disappeared from my office, and I
was cajoled about it frequently. I by the way, hate bannanas.............

Lou Solebello
-----Original Message-----
} From: L R MELSEN {lmelsen-at-emory.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Sat Apr 01 14:14:08 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Apr 2000 10:13:34 -0500
Subject: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Here is a "just off the press" example (but this does come up at least once
a year):

I fielded a phone call from a distraught SEM lab manager who told me that
some months ago he put Dow Corning fluid into his diffusion pump "to save
money". And now that he has had an accident, the silicone fluid of course,
has contaminated his system, so he was asking us, "what organic solvent will
easily remove it."

He was especially upset when I told him that his EDS detector will see Si
everywhere also, because they do a lot of analyses for Si!

I won't repeat what he told me when I tried to explain the reality of his
situation......

But it does go to show that there are a lot of new people entering our
profession, some with less training and experience with vacuum than others,
so such stories are very well worth repeating.

But just out of curiosity, is there some "recommended procedure" for
removing silicone fluid from the internal parts of a column, and also
removing it from the window of an EDS detector? I presume one can always
call in an outside service provider with experienced people but a lot of
users out there just don't have the budget for something like that. But
they do have a good supply of student manpower.

While we are on the subject of silicone, a few weeks ago a well known TEM
user got me on the phone to say "hello" and commented that he had just
placed an order for silicone grease and yes, he said, he was going to be
using it on the o rings of his column. I told him I thought that he should
be using other greases and his response was "don't listen to dogma, I
thought you read the listserver!". Am I correct, namely that one should not
be using silicone grease on the o rings of a column instrument?

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Sat Apr 01 14:14:09 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Sat, 01 Apr 2000 12:22:10 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am enjoying the "horror stories" (don't we all enjoy hearing about
someone else's stupid mistake) and thought I would share one from the
"software" category.

I had just joined Northern Scientific (later Tracor Northern, now Noran)
and was writing EDS software for our first computerized analyzer (the
venerable NS-880) -- the year was 1972, and my software, like any of
the stuff being produced in those years where we wrote software on paper
tape for machines with 4K memories, just wasn't all that "user
friendly", so it was not unusual to have to walk people through
difficult command sequences. Then too, computers were pretty new to
everyone and people sometimes made silly-sounding mistakes out of pure
inexperience (one highly intelligent customer, when instructed to 'type
a space', dutifully typed "A SPACE" on the keyboard). One particular
customer, however, defied all attempts at instruction and had passed
well beyond "complaining" into the realm of "abusive" and "insulting".
He insisted that he was following the instructions to the letter, but
that our miserable software consistently malfunctioned. We were unable
to duplicate the problem and finally several of us drove a considerable
distance to his lab to get to the bottom of this. We went through the
operations with him watching intently and the software performed
flawlessly, which he acknowledged. We then asked him to operate the
software himself and midway through, observed that he was responding to
one of the dialog questions with a decidedly inappropriate response. We
had him back out of this and repeat it with the correct response and the
software then ran perfectly. To which his response was to draw himself
up and state with great authority and indignation: "but when you run it
THE WAY I DO, it doesn't work!"

Fred Schamber
RJ Lee Instruments Limited



From daemon Sat Apr 01 14:14:09 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:21 -0600
Subject: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: pink-at-memphis.magibox.net

Name: Bill Cupo

Question: Many years ago the museum bought an AO microscope with an
Expostar Shutter control.
It appears that the control box (model 1190) has been lost.
Is there anyway to buy a replacement?
Does the company still exist? Is the microscope manufactored under another
name?

Any assistance will be greatly appreciated.

Bill Cupo
Exhibits Media Specialist

---------------------------------------------------------------------------





From daemon Sat Apr 01 14:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:01:46 -0600
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:21 -0600
Subject: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: pink-at-memphis.magibox.net

Name: Bill Cupo

Question: Many years ago the museum bought an AO microscope with an
Expostar Shutter control.
It appears that the control box (model 1190) has been lost.
Is there anyway to buy a replacement?
Does the company still exist? Is the microscope manufactored under another
name?

Any assistance will be greatly appreciated.

Bill Cupo
Exhibits Media Specialist

---------------------------------------------------------------------------





From daemon Sat Apr 01 14:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:59 -0600
Subject: XIth International Congress of Histochemistry and Cytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: george.bou-gharios-at-csc.mrc.ac.uk

I am writing to you in my capacity as secretary general of the XIth
International Congress of Histochemistry and Cytochemistry which is held in
YORK, UK 3-8 July 2000.
The above mentioned Congress is Hosted by the Royal Microscopical Society
(www.rms.org.uk) and n exciting programme has been put together which can
be seen on

www.med.ic.ac.uk/external/ichc_2000/


Dr George Bou-Gharios
Muscle Cell Biology Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital
Du Cane Road
London W12 0NN
Tel: 0181-383 8261
Fax: 0181- 383 8264
email: george.bou-gharios-at-csc.mrc.ac.uk





From daemon Sat Apr 01 14:14:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:03:50 -0600
Subject: GIF: angles calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: picomagic-at-canada.com






Originally Posted by: shens-at-focus.ruca.ua.ac.be



Hi,

How we can experimentally measure the collection angle when
working with GIF? In other words, how to measure magnification
between the plane of negatives and the plane of GIF entrance? Using
a CCD camera? But normally a CCD camera magnifies an image 20
times of that on a negative, so pictures become uncomparible.

Kind Regards,

Steven





From daemon Sat Apr 01 14:14:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:59 -0600
Subject: XIth International Congress of Histochemistry and Cytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: george.bou-gharios-at-csc.mrc.ac.uk

I am writing to you in my capacity as secretary general of the XIth
International Congress of Histochemistry and Cytochemistry which is held in
YORK, UK 3-8 July 2000.
The above mentioned Congress is Hosted by the Royal Microscopical Society
(www.rms.org.uk) and n exciting programme has been put together which can
be seen on

www.med.ic.ac.uk/external/ichc_2000/


Dr George Bou-Gharios
Muscle Cell Biology Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital
Du Cane Road
London W12 0NN
Tel: 0181-383 8261
Fax: 0181- 383 8264
email: george.bou-gharios-at-csc.mrc.ac.uk





From daemon Sat Apr 01 14:14:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:09 -0600
Subject: Administrivia: Oopps.. Nestor broke things

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: picomagic-at-canada.com





Colleagues....

In trying to adapt the SPAM filter last night to catch the latest
porn site posting I accidently broke the software. Most messages
got rejected after ~ 5 pm Chicago time. I will try to repost
those messages , however, I may have missed a few. If you
dont' see your posting by the end of the day, please resend it
and accept my apology.

Nestor
Your Friendly Neighborhood SysOp





From daemon Sat Apr 01 14:14:12 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:09 -0600
Subject: Administrivia: Oopps.. Nestor broke things

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

In trying to adapt the SPAM filter last night to catch the latest
porn site posting I accidently broke the software. Most messages
got rejected after ~ 5 pm Chicago time. I will try to repost
those messages , however, I may have missed a few. If you
dont' see your posting by the end of the day, please resend it
and accept my apology.

Nestor
Your Friendly Neighborhood SysOp





From daemon Sat Apr 01 14:14:12 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:03:50 -0600
Subject: GIF: angles calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally Posted by: shens-at-focus.ruca.ua.ac.be



Hi,

How we can experimentally measure the collection angle when
working with GIF? In other words, how to measure magnification
between the plane of negatives and the plane of GIF entrance? Using
a CCD camera? But normally a CCD camera magnifies an image 20
times of that on a negative, so pictures become uncomparible.

Kind Regards,

Steven





From daemon Sat Apr 01 14:14:13 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:01:46 -0600
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:13 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:04:30 -0600
Subject: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: bart.depauw-at-rug.ac.be


Hello,

I would like to know why they use Argon in the metal coating devices. At
our laboratory, we don't use Argon, we use air. What is the advantage of
using Argon ?
De Pauw Bart
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90





From daemon Sat Apr 01 14:14:14 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:04:30 -0600
Subject: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: bart.depauw-at-rug.ac.be


Hello,

I would like to know why they use Argon in the metal coating devices. At
our laboratory, we don't use Argon, we use air. What is the advantage of
using Argon ?
De Pauw Bart
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90





From daemon Sat Apr 01 14:14:14 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:02:50 -0600
Subject: CPD testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: cgarber-at-2spi.com



-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

As further comment on the pressure testing of the Polaron ® CPD units, I
have been asked to submit the following statement from David Cheshire, a
Manufacturing Manager at VG Microtech, the manufacturers of the Polaron
brand of critical point dryers:
=================================================
We use an independent test facility that has amongst its credentials the
following international approvals : NAMAS, UKAS, NATA. The test
certificates issued by ERA are for each individual chamber and we have
copies of all these. The test itself is a sustained 50% overpressure (2000
psi) for 1 minute. Obviously any leakage would lead to failure and non
certification. In the past units have been overpressured to 2500 and 3000
psi.

I think it is highly unlikely that a "local dive shop" would have the
facilities and wherewithal to support the standards imposed by the various
associations.

Incidentally the window is not quartz but a far denser and tougher material
specifically manufactured for high pressure use.

Best regards
Dave Cheshire

Polaron Range Development Manager
VG Microtech
The Birches Industrial Estate,
Imberhorne Lane,
East Grinstead,
West Sussex.
RH19 1UB.
UK.
===================================================
I believe there was some concern about having this kind of testing, if it
was to be done, in any kind of facility that was not certified according to
the bodies mentioned in David's message.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Apr 01 14:14:15 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:02:50 -0600
Subject: CPD testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: cgarber-at-2spi.com



-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

As further comment on the pressure testing of the Polaron ® CPD units, I
have been asked to submit the following statement from David Cheshire, a
Manufacturing Manager at VG Microtech, the manufacturers of the Polaron
brand of critical point dryers:
=================================================
We use an independent test facility that has amongst its credentials the
following international approvals : NAMAS, UKAS, NATA. The test
certificates issued by ERA are for each individual chamber and we have
copies of all these. The test itself is a sustained 50% overpressure (2000
psi) for 1 minute. Obviously any leakage would lead to failure and non
certification. In the past units have been overpressured to 2500 and 3000
psi.

I think it is highly unlikely that a "local dive shop" would have the
facilities and wherewithal to support the standards imposed by the various
associations.

Incidentally the window is not quartz but a far denser and tougher material
specifically manufactured for high pressure use.

Best regards
Dave Cheshire

Polaron Range Development Manager
VG Microtech
The Birches Industrial Estate,
Imberhorne Lane,
East Grinstead,
West Sussex.
RH19 1UB.
UK.
===================================================
I believe there was some concern about having this kind of testing, if it
was to be done, in any kind of facility that was not certified according to
the bodies mentioned in David's message.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Apr 01 14:14:15 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:24:27 -0600
Subject: IUMAS 2000 Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: jfmjfm-at-engin.umich.edu



Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"





From daemon Sat Apr 01 14:14:15 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:24:27 -0600
Subject: IUMAS 2000 Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: jfmjfm-at-engin.umich.edu



Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"





From daemon Sat Apr 01 14:14:16 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 31 Mar 2000 09:57:35 -0400
Subject: RE: Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The problem with Ni grids is that Ni is a magnetic metal, and so Ni grids
can give all kinds of trouble. We used to use them back in the early days
when they were about all that was available, and when resolution of EMs
wasn't so great; now-a-days they have lost their popularity.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Sat Apr 01 14:14:16 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 31 Mar 2000 09:57:35 -0400
Subject: RE: Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The problem with Ni grids is that Ni is a magnetic metal, and so Ni grids
can give all kinds of trouble. We used to use them back in the early days
when they were about all that was available, and when resolution of EMs
wasn't so great; now-a-days they have lost their popularity.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Sat Apr 01 14:14:16 2000



From: Christoph Benning :      benning-at-pilot.msu.edu
Date: Fri, 31 Mar 2000 09:46:29 -0500
Subject: TEM-Plant Root Plastids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there:

I would like to know whether the ratio of plastidic to extraplastidic
membranes changes in roots of Arabidopsis or any other plant in response to
phosphate starvation. Does anyone know of a TEM ultrastructural
morphometric analysis or any other type of analysis towards this end?

Thanks, Christoph Benning

Christoph Benning

Assistant Professor
Dept. of Biochemistry
Michigan State University
East Lansing, MI 48824-1319
USA

Phone: (517) 355-1609
Fax: (517)-353-9334
http://www.bch.msu.edu




From daemon Sat Apr 01 14:14:17 2000



From: Christoph Benning :      benning-at-pilot.msu.edu
Date: Fri, 31 Mar 2000 09:46:29 -0500
Subject: TEM-Plant Root Plastids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there:

I would like to know whether the ratio of plastidic to extraplastidic
membranes changes in roots of Arabidopsis or any other plant in response to
phosphate starvation. Does anyone know of a TEM ultrastructural
morphometric analysis or any other type of analysis towards this end?

Thanks, Christoph Benning

Christoph Benning

Assistant Professor
Dept. of Biochemistry
Michigan State University
East Lansing, MI 48824-1319
USA

Phone: (517) 355-1609
Fax: (517)-353-9334
http://www.bch.msu.edu




From daemon Sat Apr 01 14:14:19 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 31 Mar 2000 11:23:36 -0500 (Eastern Standard Time)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had one faculty operator several years ago who after a SINGLE lesson
on the Phillips 200, decided to come back late at night and look some
more. After taking some images, he couldn't get the door to the camera
chamber off, since he had failed to vent it. After rummaging through
the lab, he found a large screwdriver and commenced to pry the camera
door off, unconcerned about the marks and gouges that he was putting in
the brass. Needless to say, when the vacuum was finally broken, the
mercury diffusion pump became very unhappy. Eventually we were able to
replace the instrument and the user finally left after being denied
tenure.

W. L. Steffens, Ph.D
Dept. of Pathology
University of Georgia




From daemon Sat Apr 01 14:14:19 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:02:39 -0800
Subject: Need a non-fluorescing plastic slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

This is a posting for a colleague.

He needs a source of plastic slides and/or film which can be machined and
which do not fluoresce. Anybody have any ideas? Contact me offline.

Many thanks
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Sat Apr 01 14:14:20 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:02:39 -0800
Subject: Need a non-fluorescing plastic slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

This is a posting for a colleague.

He needs a source of plastic slides and/or film which can be machined and
which do not fluoresce. Anybody have any ideas? Contact me offline.

Many thanks
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Sat Apr 01 14:14:20 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 31 Mar 2000 11:23:36 -0500 (Eastern Standard Time)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had one faculty operator several years ago who after a SINGLE lesson
on the Phillips 200, decided to come back late at night and look some
more. After taking some images, he couldn't get the door to the camera
chamber off, since he had failed to vent it. After rummaging through
the lab, he found a large screwdriver and commenced to pry the camera
door off, unconcerned about the marks and gouges that he was putting in
the brass. Needless to say, when the vacuum was finally broken, the
mercury diffusion pump became very unhappy. Eventually we were able to
replace the instrument and the user finally left after being denied
tenure.

W. L. Steffens, Ph.D
Dept. of Pathology
University of Georgia




From daemon Sat Apr 01 14:14:21 2000



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 31 Mar 2000 10:11:18 -0600
Subject: RE: nickel grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} ----------
} From: Springett, Margaret J.
} Sent: Friday, March 31, 2000 11:04 AM
} To: 'springett.margaret-at-mayo.edu'
} Subject: nickel grids
}
} I do an extensive amount of immunolabeling, and nickel grids are
} economical,
} but two factors need to be considered on a daily basis. The fact that
} they
} are magnetic can be an advantage as well as a detriment. Their magnetic
} quality allows one to rinse grids floating on a bubble with a magnetic
} stir
} plate, this has been demonstrated in Dr. Bendyan's workshops on
} goldlabeling
} techniques. When trying to retreive a stubborn grid out of a grid box, a
} magnetic tweezer is "heaven sent". Now about magnetic grids in the scope,
} if you stigmate the scope after fine-focusing, your micrograph will be
} focused. I have used these techniques to produce excellent micrographs of
} caveolae at better than 100K magnification. For our use gold grids are
} too
} expensive, so "work around methods" with nickel grids are necessary.
} Marge
}
}
Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation and Clinic
Rochester, MN 55905






From daemon Sat Apr 01 14:14:21 2000



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 31 Mar 2000 10:11:18 -0600
Subject: RE: nickel grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} ----------
} From: Springett, Margaret J.
} Sent: Friday, March 31, 2000 11:04 AM
} To: 'springett.margaret-at-mayo.edu'
} Subject: nickel grids
}
} I do an extensive amount of immunolabeling, and nickel grids are
} economical,
} but two factors need to be considered on a daily basis. The fact that
} they
} are magnetic can be an advantage as well as a detriment. Their magnetic
} quality allows one to rinse grids floating on a bubble with a magnetic
} stir
} plate, this has been demonstrated in Dr. Bendyan's workshops on
} goldlabeling
} techniques. When trying to retreive a stubborn grid out of a grid box, a
} magnetic tweezer is "heaven sent". Now about magnetic grids in the scope,
} if you stigmate the scope after fine-focusing, your micrograph will be
} focused. I have used these techniques to produce excellent micrographs of
} caveolae at better than 100K magnification. For our use gold grids are
} too
} expensive, so "work around methods" with nickel grids are necessary.
} Marge
}
}
Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation and Clinic
Rochester, MN 55905






From daemon Sat Apr 01 14:14:22 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 31 Mar 2000 09:20:25 -0700
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We once had a student twist the condenser control knob off a TEM. When asked
to turn the knob clockwise, he just kept turning, until he handed me the
knob.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Friday, March 31, 2000 7:02 AM
To: Microscopy-at-sparc5.microscopy.com



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:23 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 31 Mar 2000 09:20:25 -0700
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We once had a student twist the condenser control knob off a TEM. When asked
to turn the knob clockwise, he just kept turning, until he handed me the
knob.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Friday, March 31, 2000 7:02 AM
To: Microscopy-at-sparc5.microscopy.com



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:23 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 11:43:34 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We get our Ni/holey carbon films from SPI, at http://www.2spi.com/.
They will prepare excellent holey carbon films on just about any
available grid material (for a price of course...).

Larry





} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Sat Apr 01 14:14:24 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 11:43:34 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We get our Ni/holey carbon films from SPI, at http://www.2spi.com/.
They will prepare excellent holey carbon films on just about any
available grid material (for a price of course...).

Larry





} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Sun Apr 02 16:44:01 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 31 Mar 2000 12:27:46 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor J. Zaluzec wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Originally posted by: willem.erasmus-at-sasol.com
}
} Dear fellow microscopists
}
} Does anybody know where I can purchase Ni TEM grids with a holey carbon film
} ? I can find plenty of copper grids, but the Ni grids seem to be hard to
} find.
}
} Thanks
} W. Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 9603954
} Fax : +27 +16 9602826
} E-mail : willem.erasmus-at-sasol.com

We at Ladd Reseach can supply you with these, as would most of the other
supply houses that make their own holey film. Please let us know what
size mesh you want and a quantity and we can quote you.

Debbie Sicard
Sales Manager

Disclaimer: Ladd Research is a vendor that sells EM supplies.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Sun Apr 02 16:44:01 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 31 Mar 2000 12:27:46 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor J. Zaluzec wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Originally posted by: willem.erasmus-at-sasol.com
}
} Dear fellow microscopists
}
} Does anybody know where I can purchase Ni TEM grids with a holey carbon film
} ? I can find plenty of copper grids, but the Ni grids seem to be hard to
} find.
}
} Thanks
} W. Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 9603954
} Fax : +27 +16 9602826
} E-mail : willem.erasmus-at-sasol.com

We at Ladd Reseach can supply you with these, as would most of the other
supply houses that make their own holey film. Please let us know what
size mesh you want and a quantity and we can quote you.

Debbie Sicard
Sales Manager

Disclaimer: Ladd Research is a vendor that sells EM supplies.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Sun Apr 02 16:44:02 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 31 Mar 2000 12:34:21 -0600
Subject: TEM/peroxidase immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently using the peroxidase/DAB pre-embedding method for
localization of a novel extracellular matrix protein. I am using 40 micron
vibratome sections. While my results give some extracellular labelling,
most of the labelling is associated with dendritic microtubules. Has anyone
experienced non-specific labelling of microtubules?



From daemon Sun Apr 02 16:44:02 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 31 Mar 2000 12:34:21 -0600
Subject: TEM/peroxidase immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently using the peroxidase/DAB pre-embedding method for
localization of a novel extracellular matrix protein. I am using 40 micron
vibratome sections. While my results give some extracellular labelling,
most of the labelling is associated with dendritic microtubules. Has anyone
experienced non-specific labelling of microtubules?



From daemon Sun Apr 02 16:44:03 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Mar 2000 13:39:47 -0500
Subject: Carbon coated Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Erasmus asked:
================================================
Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.
=================================================
Some times the coating of Ni grids is a bit more difficult than the coating
of copper grids. This translates to somewhat lower yields and sometimes
higher prices. However, SPI Supplies regularly produces custom coated Ni
grids on whatever mesh and grid the customer specifies and at only a slight
price premium over copper.

Contact me off line for details or visit our webpage URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Some of the other major suppliers of consumables also will coat Ni grids, or
at least that is my understanding.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:03 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Mar 2000 13:39:47 -0500
Subject: Carbon coated Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Erasmus asked:
================================================
Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.
=================================================
Some times the coating of Ni grids is a bit more difficult than the coating
of copper grids. This translates to somewhat lower yields and sometimes
higher prices. However, SPI Supplies regularly produces custom coated Ni
grids on whatever mesh and grid the customer specifies and at only a slight
price premium over copper.

Contact me off line for details or visit our webpage URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Some of the other major suppliers of consumables also will coat Ni grids, or
at least that is my understanding.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:03 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 Mar 2000 08:45:16 -1000 (HST)
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One day a user of our TEM complained that she could see the beam with the
specimen out, but as soon as she put in a grid the screen went
black. After playing with it awhile and ruling out magnetism or something
on the grid holder, I decided that there must be some small magnetic
particle in the stage area that was being pushed around by the grid
holder. Of course the service technician I got by phone wanted me to
begin taking the column apart from the gun on down, but I grew impatient
with this and soon went straight for the stage. I figured I would be
looking for some small metal sliver. Imagine my surprise when I found an
entire plastic pipettortip lying across the bore through the
anticontamination plate in the column! It seems the previous user had
been using these tips as forceps covers, and mysteriously lost one the day
before. It had gone into the column through the airlock with her grid,
not impossible with the Zeiss 10 top-loading system. Although not the
only strange thing I have found in that microscope's column, at about an
inch and a half long it is the largest.

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Sun Apr 02 16:44:04 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 Mar 2000 08:45:16 -1000 (HST)
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One day a user of our TEM complained that she could see the beam with the
specimen out, but as soon as she put in a grid the screen went
black. After playing with it awhile and ruling out magnetism or something
on the grid holder, I decided that there must be some small magnetic
particle in the stage area that was being pushed around by the grid
holder. Of course the service technician I got by phone wanted me to
begin taking the column apart from the gun on down, but I grew impatient
with this and soon went straight for the stage. I figured I would be
looking for some small metal sliver. Imagine my surprise when I found an
entire plastic pipettortip lying across the bore through the
anticontamination plate in the column! It seems the previous user had
been using these tips as forceps covers, and mysteriously lost one the day
before. It had gone into the column through the airlock with her grid,
not impossible with the Zeiss 10 top-loading system. Although not the
only strange thing I have found in that microscope's column, at about an
inch and a half long it is the largest.

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Sun Apr 02 16:44:04 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 31 Mar 2000 14:48:58 -0500 (EST)
Subject: Core Facility solvency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscpy Core Managers,
Are there any EM/Confocal Microscope facilities within academia that
are running in the black or at least breaking even without a subsidy. If
so what are your rates and overhead, are your salaries included? Also are
there any companies or facilities that do tele-microscopy? All info will be
shared with the listserver, unless confidentiality is requested. Thank You.

Michael Delannoy
Basic Science Microscopy Facility
JHMI




From daemon Sun Apr 02 16:44:05 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 31 Mar 2000 14:48:58 -0500 (EST)
Subject: Core Facility solvency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscpy Core Managers,
Are there any EM/Confocal Microscope facilities within academia that
are running in the black or at least breaking even without a subsidy. If
so what are your rates and overhead, are your salaries included? Also are
there any companies or facilities that do tele-microscopy? All info will be
shared with the listserver, unless confidentiality is requested. Thank You.

Michael Delannoy
Basic Science Microscopy Facility
JHMI




From daemon Sun Apr 02 16:44:05 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 31 Mar 2000 15:46:00 -0600
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Sun Apr 02 16:44:06 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 31 Mar 2000 15:46:00 -0600
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Sun Apr 02 16:44:06 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Fri, 31 Mar 2000 16:48:24 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my second go around with this, I got the Nestor porno bump this
morning.

I had a student using my JEOL 100 CX II; loading and looking around went
well. When she went to remove the holder she got half way out and yanked it
toward her. This action put a 70 degree bend in the holder. I could never
get the Z height to behave after that for some odd reason.

The other disaster was with my Philips 300, complete with Mercury Dif pump
at that time. I needed to do some anode cleaning, so without deflating the
air table I got on the console finished my work and got off. When the scope
lurched to the right it dumped the contents of the mercury pump into the oil
pump. This sent a cloud of mercury up the column creating an alloy wherever
it went.

Believe it or not the scope is in fine working order with not a trace of
mercury left in in the column. How did I do it? If you can figure it out I
will send the winner a Lucent/Bell Labs brief case...at my cost of course.

John Grazul
Lucent Technologies

"W. L. Steffens" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I had one faculty operator several years ago who after a SINGLE lesson
} on the Phillips 200, decided to come back late at night and look some
} more. After taking some images, he couldn't get the door to the camera
} chamber off, since he had failed to vent it. After rummaging through
} the lab, he found a large screwdriver and commenced to pry the camera
} door off, unconcerned about the marks and gouges that he was putting in
} the brass. Needless to say, when the vacuum was finally broken, the
} mercury diffusion pump became very unhappy. Eventually we were able to
} replace the instrument and the user finally left after being denied
} tenure.
}
} W. L. Steffens, Ph.D
} Dept. of Pathology
} University of Georgia




From daemon Sun Apr 02 16:44:07 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Fri, 31 Mar 2000 16:48:24 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my second go around with this, I got the Nestor porno bump this
morning.

I had a student using my JEOL 100 CX II; loading and looking around went
well. When she went to remove the holder she got half way out and yanked it
toward her. This action put a 70 degree bend in the holder. I could never
get the Z height to behave after that for some odd reason.

The other disaster was with my Philips 300, complete with Mercury Dif pump
at that time. I needed to do some anode cleaning, so without deflating the
air table I got on the console finished my work and got off. When the scope
lurched to the right it dumped the contents of the mercury pump into the oil
pump. This sent a cloud of mercury up the column creating an alloy wherever
it went.

Believe it or not the scope is in fine working order with not a trace of
mercury left in in the column. How did I do it? If you can figure it out I
will send the winner a Lucent/Bell Labs brief case...at my cost of course.

John Grazul
Lucent Technologies

"W. L. Steffens" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I had one faculty operator several years ago who after a SINGLE lesson
} on the Phillips 200, decided to come back late at night and look some
} more. After taking some images, he couldn't get the door to the camera
} chamber off, since he had failed to vent it. After rummaging through
} the lab, he found a large screwdriver and commenced to pry the camera
} door off, unconcerned about the marks and gouges that he was putting in
} the brass. Needless to say, when the vacuum was finally broken, the
} mercury diffusion pump became very unhappy. Eventually we were able to
} replace the instrument and the user finally left after being denied
} tenure.
}
} W. L. Steffens, Ph.D
} Dept. of Pathology
} University of Georgia




From daemon Sun Apr 02 16:44:08 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Fri, 31 Mar 2000 16:40:27 -0600
Subject: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once, over a decade back, I was working EM and Med Tech at the same
time at a local hospital..

We had a CAP inspection, the officers were from another hospital in the state.


The question I was expected to reason and answer with a straight face was:


* What other protective measures besides standing behind a lead
wall do you take when you put the glass slide into the TEM, to
protect yourself from the flying electrons???



seriously!

I was rather stunned, and started looking from the wall socket to
him and back, but he didn't catch the connection.


I then tried to tactfully explain that vacuum was required, and that
we really didn't need that extra protection!

It was explained to me that he did too know all about EM because he
had a one day workshop.



Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } }
Lou Ann Miller
Service Supervisor

Rm 1108 VMBSBld.
College of Veterinary Medicine
Veterinary Biosciences
2001 S. Lincoln Ave
Urbana, IL 61802

Phone: 217-244-1567
Fax: 217-244-1652

lamiller-at-uiuc.edu
lamiller-at-net66.com
turtle_lam-at-yahoo.com

Web Pages:
Lab Page
http://treefrog.cvm.uiuc.edu
Centeral States Microscopy Page
http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page
http://treefrog.cvm.uiuc.edu/lam



From daemon Sun Apr 02 16:44:08 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Fri, 31 Mar 2000 16:40:27 -0600
Subject: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once, over a decade back, I was working EM and Med Tech at the same
time at a local hospital..

We had a CAP inspection, the officers were from another hospital in the state.


The question I was expected to reason and answer with a straight face was:


* What other protective measures besides standing behind a lead
wall do you take when you put the glass slide into the TEM, to
protect yourself from the flying electrons???



seriously!

I was rather stunned, and started looking from the wall socket to
him and back, but he didn't catch the connection.


I then tried to tactfully explain that vacuum was required, and that
we really didn't need that extra protection!

It was explained to me that he did too know all about EM because he
had a one day workshop.



Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } }
Lou Ann Miller
Service Supervisor

Rm 1108 VMBSBld.
College of Veterinary Medicine
Veterinary Biosciences
2001 S. Lincoln Ave
Urbana, IL 61802

Phone: 217-244-1567
Fax: 217-244-1652

lamiller-at-uiuc.edu
lamiller-at-net66.com
turtle_lam-at-yahoo.com

Web Pages:
Lab Page
http://treefrog.cvm.uiuc.edu
Centeral States Microscopy Page
http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page
http://treefrog.cvm.uiuc.edu/lam



From daemon Sun Apr 02 16:44:08 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 31 Mar 2000 17:58:37 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We once had an operator on our JEOL 840A SEM complain about her
} sample moving. Her sample was samples of vacuum grease she had gold
} coated and was attempting to image in the SEM. When the electron
} beam heated the grease it cracked up the gold coating and charged.
} She was using the SEM to look for silicon in various greases. I
} suggested she use a different technique so we did not end up with
} vaporized grease inside the SEM.

David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Sun Apr 02 16:44:09 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 31 Mar 2000 17:58:37 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We once had an operator on our JEOL 840A SEM complain about her
} sample moving. Her sample was samples of vacuum grease she had gold
} coated and was attempting to image in the SEM. When the electron
} beam heated the grease it cracked up the gold coating and charged.
} She was using the SEM to look for silicon in various greases. I
} suggested she use a different technique so we did not end up with
} vaporized grease inside the SEM.

David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Sun Apr 02 16:44:09 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 Mar 2000 16:48:19 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems everyone has one of these. I had a person who one day
complained of a burning smell while she was at the scope. I went to
investigate and when I arrived in the room there was no smell... burning
or otherwise. Several more times that session she came and got me to
investigate this burning smell in the TEM room. This continued for a
couple of sessions. I did go and investigate because after all, who
wants their TEM on fire? I honestly didn't know what to do about it and
was beginning to seriously question her sanity. Finally she convinced
me to sit with her while she operated the scope and sure enough there
was this faint smell of something burning! I jumped up and tried to
find it, but it had gone away. So...she sat down again and began to
work and the smell, like plastic kitchenware burning in a dishwasher,
came back. Again I jump up and look around....to no avail! I was
curious now. The burning smell only happens when she is sitting at the
scope. Not being a big believer in spontaneous combustion, there had to
be an answer! There was. As part of the TEM course I take the kick
panel off the Zeiss 10 to show the students the guts of the vacuum
system. The kick panel is right below the desk area where you sit up to
the microscope. What she was doing was placing her tennis shoe on the
heater of the diffusion pump and the plastic/rubber would melt and burn
a bit -- just enough to give the room a burning smell. She never did
notice her foot getting warm. When I pointed it out she did admit that
her shoe was warm and had some ugly scars on it to boot! She never
returned after that semester - I guess that she had enough of EM! Now I
carefully point out the hazard and replace the cover quickly when we are
finished with the class.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Sun Apr 02 16:44:09 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 Mar 2000 16:48:19 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems everyone has one of these. I had a person who one day
complained of a burning smell while she was at the scope. I went to
investigate and when I arrived in the room there was no smell... burning
or otherwise. Several more times that session she came and got me to
investigate this burning smell in the TEM room. This continued for a
couple of sessions. I did go and investigate because after all, who
wants their TEM on fire? I honestly didn't know what to do about it and
was beginning to seriously question her sanity. Finally she convinced
me to sit with her while she operated the scope and sure enough there
was this faint smell of something burning! I jumped up and tried to
find it, but it had gone away. So...she sat down again and began to
work and the smell, like plastic kitchenware burning in a dishwasher,
came back. Again I jump up and look around....to no avail! I was
curious now. The burning smell only happens when she is sitting at the
scope. Not being a big believer in spontaneous combustion, there had to
be an answer! There was. As part of the TEM course I take the kick
panel off the Zeiss 10 to show the students the guts of the vacuum
system. The kick panel is right below the desk area where you sit up to
the microscope. What she was doing was placing her tennis shoe on the
heater of the diffusion pump and the plastic/rubber would melt and burn
a bit -- just enough to give the room a burning smell. She never did
notice her foot getting warm. When I pointed it out she did admit that
her shoe was warm and had some ugly scars on it to boot! She never
returned after that semester - I guess that she had enough of EM! Now I
carefully point out the hazard and replace the cover quickly when we are
finished with the class.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Sun Apr 02 16:44:10 2000



From: Amy McGough :      amcgough-at-bcm.tmc.edu
Date: Fri, 31 Mar 2000 17:50:10 -0600
Subject: Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position Announcement

Research Scientist in Macromolecular Microscopy

Purdue University Department of Biological Sciences

An opening is available immediately for a scientist specializing in
macromolecular microscopy at Purdue University. The successful
candidate would be responsible for overseeing the day-to-day
operations of the high-resolution electron microscopy facility, training
new users, and assisting outside visitors with data collection and
analysis. He/she would also be expected to work with the cryoEM
groups in the department at planning and implementing the future
directions of the facility, including the development of new
technologies for improved specimen preservation, data collection, and
analysis. The ideal candidate should complement the existing
strengths represented in the department and pursue independent
research funding.

The Department of Biological Sciences at Purdue provides a rich
intellectual environment as well as outstanding modern scientific
facilities including Philips EM420, CM200-FEG, and CM300-FEG
electron cryo-microscopes. Purdue University is home to over 50,000
students, faculty, and staff, and is located in West Lafayette, Indiana -
approximately 1 hour northwest of Indianapolis and 2.5 hours
southeast of Chicago. The area affords the advantages associated with
a major university, while providing an affordable and attractive `small
town' environment in which to live.

Requirements for this position include a Ph.D. in biophysics or one of
the related sciences, a minimum of 5 years post-doctoral work
experience in electron cryo-microscopy of biological molecules, and a
proven ability to work well with others. Preference will be given to
candidates who have successfully developed new technologies and
methodologies for high-resolution electron cryo-microscopy. Salary
will be commensurate with experience and includes full benefits.

Interested individuals should send their resume, relevant reprints, a
statement of research interests and career goals, and provide contact
information (name/phone/e-mail) for three individuals who have
agreed to serve as references to:

Dr. Timothy S. Baker (tsb-at-bragg.bio.purdue.edu)

and/or

Dr. Amy McGough (amcgough-at-bcm.tmc.edu)

Department of Biological Sciences
Lilly Hall of Life Science
Purdue University
West Lafayette, IN 47907-1392

Purdue is an equal access/equal opportunity university.



From daemon Sun Apr 02 16:44:10 2000



From: Amy McGough :      amcgough-at-bcm.tmc.edu
Date: Fri, 31 Mar 2000 17:50:10 -0600
Subject: Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position Announcement

Research Scientist in Macromolecular Microscopy

Purdue University Department of Biological Sciences

An opening is available immediately for a scientist specializing in
macromolecular microscopy at Purdue University. The successful
candidate would be responsible for overseeing the day-to-day
operations of the high-resolution electron microscopy facility, training
new users, and assisting outside visitors with data collection and
analysis. He/she would also be expected to work with the cryoEM
groups in the department at planning and implementing the future
directions of the facility, including the development of new
technologies for improved specimen preservation, data collection, and
analysis. The ideal candidate should complement the existing
strengths represented in the department and pursue independent
research funding.

The Department of Biological Sciences at Purdue provides a rich
intellectual environment as well as outstanding modern scientific
facilities including Philips EM420, CM200-FEG, and CM300-FEG
electron cryo-microscopes. Purdue University is home to over 50,000
students, faculty, and staff, and is located in West Lafayette, Indiana -
approximately 1 hour northwest of Indianapolis and 2.5 hours
southeast of Chicago. The area affords the advantages associated with
a major university, while providing an affordable and attractive `small
town' environment in which to live.

Requirements for this position include a Ph.D. in biophysics or one of
the related sciences, a minimum of 5 years post-doctoral work
experience in electron cryo-microscopy of biological molecules, and a
proven ability to work well with others. Preference will be given to
candidates who have successfully developed new technologies and
methodologies for high-resolution electron cryo-microscopy. Salary
will be commensurate with experience and includes full benefits.

Interested individuals should send their resume, relevant reprints, a
statement of research interests and career goals, and provide contact
information (name/phone/e-mail) for three individuals who have
agreed to serve as references to:

Dr. Timothy S. Baker (tsb-at-bragg.bio.purdue.edu)

and/or

Dr. Amy McGough (amcgough-at-bcm.tmc.edu)

Department of Biological Sciences
Lilly Hall of Life Science
Purdue University
West Lafayette, IN 47907-1392

Purdue is an equal access/equal opportunity university.



From daemon Sun Apr 02 16:44:10 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 31 Mar 2000 18:10:58 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bart,
I believe Argon is used because it is a larger molecule and
sputters the metal more efficiently. I also use air with no problem.
Possibly the amount of metal sputtered is slightly less per unit time.
John Hunt

On Fri, 31 Mar 2000, Nestor J. Zaluzec wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Originally posted by: bart.depauw-at-rug.ac.be
}
}
} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90
}
}
}





From daemon Sun Apr 02 16:44:11 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Fri, 31 Mar 2000 18:12:12 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057





From daemon Sun Apr 02 16:44:11 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 31 Mar 2000 18:10:58 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bart,
I believe Argon is used because it is a larger molecule and
sputters the metal more efficiently. I also use air with no problem.
Possibly the amount of metal sputtered is slightly less per unit time.
John Hunt

On Fri, 31 Mar 2000, Nestor J. Zaluzec wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Originally posted by: bart.depauw-at-rug.ac.be
}
}
} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90
}
}
}





From daemon Sun Apr 02 16:44:11 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Fri, 31 Mar 2000 18:12:12 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057





From daemon Sun Apr 02 16:44:12 2000



From: pe13-at-cam.ac.uk -at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 18:11:27 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Air contains water vapour, oxygen and carbon dioxide all of which can be
broken down in the plasma generated in a sputter coater and gve rise to
nasty active species which can damage delicate samples. Argon is used
because of its relative high atomic number and this ensures there is
multiple scattering of the sputtered metal. There is a Zenon sputter coater
on the market which should sputter even better but I have no experience
with it. Come to the Lehigh Short Course(contact {slc6-at-lehigh.edu) and we
will tell you all about it.

Patrick Echlin
Cambridge





From daemon Sun Apr 02 16:44:12 2000



From: pe13-at-cam.ac.uk -at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 18:11:27 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Air contains water vapour, oxygen and carbon dioxide all of which can be
broken down in the plasma generated in a sputter coater and gve rise to
nasty active species which can damage delicate samples. Argon is used
because of its relative high atomic number and this ensures there is
multiple scattering of the sputtered metal. There is a Zenon sputter coater
on the market which should sputter even better but I have no experience
with it. Come to the Lehigh Short Course(contact {slc6-at-lehigh.edu) and we
will tell you all about it.

Patrick Echlin
Cambridge





From daemon Sun Apr 02 16:44:16 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.




From daemon Sun Apr 02 16:44:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.




From daemon Sun Apr 02 16:44:24 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 3 Apr 2000 00:00:21 +1000
Subject: RE: LM/EM Embedding Oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I appreciate the comments by David Cheshire. Perhaps he could elaborate why a
place (not necessarily a Dive Shop) certified for the testing of diving
cylinders and regulators could not cope with a CPD? Is the implication that
these testing places can not be trusted (sorry divers), or that a CPD is more
complex than other equipment, or, horror, could these places be cheaper to get
a CPD tested. and certified. Surely, hydrostatic testing is rather similar and
certification and the relevant legislation would be for pressure vessels and
not just for CPD.

I still believe that testing of CPD is counter-productive, but if required by
legislation, then there is no option available.

I believe that CPD windows used to be quartz. This may well have changed and
some manufacturers may now use other material e.g."bullet proof glass", which
of course is a plastic. David Cheshire would have been more helpful to devulge
which material is used. This would be of great interest because some people
have used organic solvents as an intermediate fluid. In that case it would be
important to know what solvents affect this "far denser and tougher material".
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

-----Original Message-----
Sent: Saturday, April 01, 2000 12:03 AM
To: MICROSCOPY BB


A couple of suggestions that may help your collogue and others in regards to
ovens. Please note that PST sells ovens, but not to North America (wrong volts,
costly shipping).

For resin curing most people purchase the cheapest oven type, which relies on
convection currents to achieve reasonably even temperatures. Fan forced and
jacketed ovens have greater temperature uniformity within the chamber. (See
diagrams of oven types in our online: Home} Contents} E3} "click here" link) Its
wise to place specimens always in a similar position within the chamber.

A thermostat control the heating elements and commonly they switch within 1.5
degree C. Metals absorb heat faster and conduct heat faster into specimens. It
is quite possible to part-melt polyethylene capsules where they are in contact
with metal. The simple solution is to place the specimens on a piece of wood or
thick cardboard.

The offending oven may have a thermostat that switches to its own strange
rhythm. More likely though, it has an unacceptably wide range when turning the
element on and off. A possible solution to this problem is to enclose the
specimen in an insulating box within the oven. This should cause the highs and
lows to be leveled. That insulating box could be a small lid-less cardbox,
upended over the specimen and its insulating support.

Uneven polymerisation is often due to varying times within the oven. An easy
solution is a "lamplighter" timer. Plug the oven into this 24 hour timer and
adjust the timer to come on at say 5 pm and switch off at 8 am. Specimen can be
placed in the oven at any time and removed any time during the next day and
cure for the same number of hours. Fewer fumes within the lab is another
benefit.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, March 31, 2000 4:02 AM, Paula Allan-Wojtas
[SMTP:ALLANWOJTASP-at-EM.AGR.CA] wrote:
}
}
} Hi, All,
}
} I am posting a message for a colleague who does not subscribe to this list.
}
} He is looking for small embedding oven which can polymerize resins in the
} range of 40?-70?C, but which has a very precise temperature control (holds
} the temperature well, with very little fluctuation during polymerization). He
} is having problems with the oven he presently has because the temperature is
} not controlled well enough, and he is getting uneven polymerization.
}
} Another condition is that he is in the middle of a project and needs the oven
} right away. He had an oven in mind, but it could not be delivered for 6
} weeks!!!
}
} Any suggestions from users or vendors are welcome. Please contact me offline,
} and I'll forward the replies to my colleague.
}
} Thanks again for all your help.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist, Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}



From daemon Sun Apr 02 16:44:30 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger




From daemon Sun Apr 02 16:44:30 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Apr 2000 10:13:34 -0500
Subject: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Here is a "just off the press" example (but this does come up at least once
a year):

I fielded a phone call from a distraught SEM lab manager who told me that
some months ago he put Dow Corning fluid into his diffusion pump "to save
money". And now that he has had an accident, the silicone fluid of course,
has contaminated his system, so he was asking us, "what organic solvent will
easily remove it."

He was especially upset when I told him that his EDS detector will see Si
everywhere also, because they do a lot of analyses for Si!

I won't repeat what he told me when I tried to explain the reality of his
situation......

But it does go to show that there are a lot of new people entering our
profession, some with less training and experience with vacuum than others,
so such stories are very well worth repeating.

But just out of curiosity, is there some "recommended procedure" for
removing silicone fluid from the internal parts of a column, and also
removing it from the window of an EDS detector? I presume one can always
call in an outside service provider with experienced people but a lot of
users out there just don't have the budget for something like that. But
they do have a good supply of student manpower.

While we are on the subject of silicone, a few weeks ago a well known TEM
user got me on the phone to say "hello" and commented that he had just
placed an order for silicone grease and yes, he said, he was going to be
using it on the o rings of his column. I told him I thought that he should
be using other greases and his response was "don't listen to dogma, I
thought you read the listserver!". Am I correct, namely that one should not
be using silicone grease on the o rings of a column instrument?

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:30 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, March 31, 2000 9:26 PM
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My story is not as much of a horror as it is embarassing. Several years
back I received a package of samples from a regular client without any paper
work describing what the samples were. This is not unusual since we
frequently recieve unknowns. I proceeded to open the package and was
abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
of the samples was a vial of concentrated bannana flavoring. It was months
(almost a year) before the odor completely disappeared from my office, and I
was cajoled about it frequently. I by the way, hate bannanas.............

Lou Solebello
-----Original Message-----
} From: L R MELSEN {lmelsen-at-emory.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Sun Apr 02 16:44:31 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger




From daemon Sun Apr 02 16:44:31 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Apr 2000 10:13:34 -0500
Subject: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Here is a "just off the press" example (but this does come up at least once
a year):

I fielded a phone call from a distraught SEM lab manager who told me that
some months ago he put Dow Corning fluid into his diffusion pump "to save
money". And now that he has had an accident, the silicone fluid of course,
has contaminated his system, so he was asking us, "what organic solvent will
easily remove it."

He was especially upset when I told him that his EDS detector will see Si
everywhere also, because they do a lot of analyses for Si!

I won't repeat what he told me when I tried to explain the reality of his
situation......

But it does go to show that there are a lot of new people entering our
profession, some with less training and experience with vacuum than others,
so such stories are very well worth repeating.

But just out of curiosity, is there some "recommended procedure" for
removing silicone fluid from the internal parts of a column, and also
removing it from the window of an EDS detector? I presume one can always
call in an outside service provider with experienced people but a lot of
users out there just don't have the budget for something like that. But
they do have a good supply of student manpower.

While we are on the subject of silicone, a few weeks ago a well known TEM
user got me on the phone to say "hello" and commented that he had just
placed an order for silicone grease and yes, he said, he was going to be
using it on the o rings of his column. I told him I thought that he should
be using other greases and his response was "don't listen to dogma, I
thought you read the listserver!". Am I correct, namely that one should not
be using silicone grease on the o rings of a column instrument?

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:32 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, March 31, 2000 9:26 PM
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My story is not as much of a horror as it is embarassing. Several years
back I received a package of samples from a regular client without any paper
work describing what the samples were. This is not unusual since we
frequently recieve unknowns. I proceeded to open the package and was
abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
of the samples was a vial of concentrated bannana flavoring. It was months
(almost a year) before the odor completely disappeared from my office, and I
was cajoled about it frequently. I by the way, hate bannanas.............

Lou Solebello
-----Original Message-----
} From: L R MELSEN {lmelsen-at-emory.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Sun Apr 02 16:44:32 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Sat, 01 Apr 2000 12:22:10 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am enjoying the "horror stories" (don't we all enjoy hearing about
someone else's stupid mistake) and thought I would share one from the
"software" category.

I had just joined Northern Scientific (later Tracor Northern, now Noran)
and was writing EDS software for our first computerized analyzer (the
venerable NS-880) -- the year was 1972, and my software, like any of
the stuff being produced in those years where we wrote software on paper
tape for machines with 4K memories, just wasn't all that "user
friendly", so it was not unusual to have to walk people through
difficult command sequences. Then too, computers were pretty new to
everyone and people sometimes made silly-sounding mistakes out of pure
inexperience (one highly intelligent customer, when instructed to 'type
a space', dutifully typed "A SPACE" on the keyboard). One particular
customer, however, defied all attempts at instruction and had passed
well beyond "complaining" into the realm of "abusive" and "insulting".
He insisted that he was following the instructions to the letter, but
that our miserable software consistently malfunctioned. We were unable
to duplicate the problem and finally several of us drove a considerable
distance to his lab to get to the bottom of this. We went through the
operations with him watching intently and the software performed
flawlessly, which he acknowledged. We then asked him to operate the
software himself and midway through, observed that he was responding to
one of the dialog questions with a decidedly inappropriate response. We
had him back out of this and repeat it with the correct response and the
software then ran perfectly. To which his response was to draw himself
up and state with great authority and indignation: "but when you run it
THE WAY I DO, it doesn't work!"

Fred Schamber
RJ Lee Instruments Limited




From daemon Sun Apr 02 16:44:33 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Sat, 01 Apr 2000 12:22:10 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am enjoying the "horror stories" (don't we all enjoy hearing about
someone else's stupid mistake) and thought I would share one from the
"software" category.

I had just joined Northern Scientific (later Tracor Northern, now Noran)
and was writing EDS software for our first computerized analyzer (the
venerable NS-880) -- the year was 1972, and my software, like any of
the stuff being produced in those years where we wrote software on paper
tape for machines with 4K memories, just wasn't all that "user
friendly", so it was not unusual to have to walk people through
difficult command sequences. Then too, computers were pretty new to
everyone and people sometimes made silly-sounding mistakes out of pure
inexperience (one highly intelligent customer, when instructed to 'type
a space', dutifully typed "A SPACE" on the keyboard). One particular
customer, however, defied all attempts at instruction and had passed
well beyond "complaining" into the realm of "abusive" and "insulting".
He insisted that he was following the instructions to the letter, but
that our miserable software consistently malfunctioned. We were unable
to duplicate the problem and finally several of us drove a considerable
distance to his lab to get to the bottom of this. We went through the
operations with him watching intently and the software performed
flawlessly, which he acknowledged. We then asked him to operate the
software himself and midway through, observed that he was responding to
one of the dialog questions with a decidedly inappropriate response. We
had him back out of this and repeat it with the correct response and the
software then ran perfectly. To which his response was to draw himself
up and state with great authority and indignation: "but when you run it
THE WAY I DO, it doesn't work!"

Fred Schamber
RJ Lee Instruments Limited




From daemon Sun Apr 02 16:44:33 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sun, 02 Apr 2000 21:16:37 +0100
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a similar story to this one! A bright fellow from a senior
University (I would embarrass them) had a dim TEM image, so rigged up
an Anglepoise lamp to try and throw some more light on it!

Keith Ryan
Marine Biological Association
Plymouth, UK

} } } Larry Allard {l2a-at-ornl.gov} 03/31/00 06:08pm } } }
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} Humorous, but not quite horror:


The respected PhD I once worked with on our old JEOL JSM-U3 scanner,

who was having trouble making out the details of the image on the
monitor, so grabbed a flashlight and shined it at the screen so he
could see the image a little better... :-).

Larry

}
}
} Originally posted by: sryazant-at-ucla.edu
}
}
}
}
} It is not "horror" story, but a sort of... Many years ago the
colleague
} from friendly Lab visited me with great project. The idea was
simply and
} beautiful. She argues that because the image in the scope (TEM) is
green
} (green fluorescence of the screen), she wants to modify the sample
(I
} forgot what, some protein, I believe) by red-fluorescent dye to be
able to
} see on the screen of the electron microscope the "double-staining":
red on
} the green background. No comments...
}
} Sergey
}
} } Date: Thu, 30 Mar 2000 09:17:56 -0500
} } From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} } Subject: Operator Horror Stories
} } To: microscopy-at-sparc5.microscopy.com
} } Importance: Normal
} } X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release
5.0.2b
} (Intl)|18
} } January 2000) at 30/03/2000 09:18:01
} }
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com

} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

}
} -----------------------------------------------------------------------.
} }
} }
} }
} } Alan Fox, in a response to the Analytical TEM string, cautioned
the
} } question-raiser to beware of people who abuse instruments.
Perhaps Nestor
} } will forgive a little microscopy related humor and allow us to
start a
} } string on "Operator Horror Stories." Here's ours:
} }
} } The "ham-fisted user" reference made us chuckle/cringe with the
memory of
} } a guest "microscopist" in our lab who hauled himself (220 pounds
or so) out
} } of his seat by pulling on the half-inserted specimen rod, bending
it about
} } 20 degrees or so!
} }
} } Henceforth, when we saw him in the hall (he never came into the
scope room
} } again), we referred to him as "Conan the Microscopist"!
} }
} }
} }
} } Ron Anderson, IBM, Hopewell Jct., New York, USA.
anderron-at-us.ibm.com

} }
} } IBM Analytical Services; http://www.chips.ibm.com/services/asg
} }
} }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Mon Apr 03 07:53:59 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Sun, 2 Apr 2000 21:34:13 -0400 (EDT)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What in the world is an Anglepoise lamp? Is this the same as a "Gooseneck"?

Don Marshall


} From Microscopy-request-at-sparc5.microscopy.com Sun Apr 2 17:01:37 2000
}
} -----------------------------------------------------------------------.
}
}
} I have a similar story to this one! A bright fellow from a senior
} University (I would embarrass them) had a dim TEM image, so rigged up
} an Anglepoise lamp to try and throw some more light on it!
}
} Keith Ryan
} Marine Biological Association
} Plymouth, UK
}

} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
}
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} } -----------------------------------------------------------------------.
} } Humorous, but not quite horror:
}
}
} The respected PhD I once worked with on our old JEOL JSM-U3 scanner,
}
} who was having trouble making out the details of the image on the
} monitor, so grabbed a flashlight and shined it at the screen so he
} could see the image a little better... :-).
}
} Larry
}
} }
} }
} } Originally posted by: sryazant-at-ucla.edu
} }
} }
} }
} }
} } It is not "horror" story, but a sort of... Many years ago the
} colleague
} } from friendly Lab visited me with great project. The idea was
} simply and
} } beautiful. She argues that because the image in the scope (TEM) is
} green
} } (green fluorescence of the screen), she wants to modify the sample
} (I
} } forgot what, some protein, I believe) by red-fluorescent dye to be
} able to
} } see on the screen of the electron microscope the "double-staining":
} red on
} } the green background. No comments...
} }
} } Sergey
} }
} } } Date: Thu, 30 Mar 2000 09:17:56 -0500
} } } From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} } } Subject: Operator Horror Stories
} } } To: microscopy-at-sparc5.microscopy.com
} } } Importance: Normal
} } } X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release
} 5.0.2b
} } (Intl)|18
} } } January 2000) at 30/03/2000 09:18:01
} } }
} }
} } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
}
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} }
} } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } } Alan Fox, in a response to the Analytical TEM string, cautioned
} the
} } } question-raiser to beware of people who abuse instruments.
} Perhaps Nestor
} } } will forgive a little microscopy related humor and allow us to
} start a
} } } string on "Operator Horror Stories." Here's ours:
} } }
} } } The "ham-fisted user" reference made us chuckle/cringe with the
} memory of
} } } a guest "microscopist" in our lab who hauled himself (220 pounds
} or so) out
} } } of his seat by pulling on the half-inserted specimen rod, bending
} it about
} } } 20 degrees or so!
} } }
} } } Henceforth, when we saw him in the hall (he never came into the
} scope room
} } } again), we referred to him as "Conan the Microscopist"!
} } }
} } }
} } }
} } } Ron Anderson, IBM, Hopewell Jct., New York, USA.
} anderron-at-us.ibm.com
}
} } }
} } } IBM Analytical Services; http://www.chips.ibm.com/services/asg
} } }
} } }
} } }
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 423-574-4981
} 423-574-4913 Fax
} l2a-at-ornl.gov
}
}



From daemon Mon Apr 03 07:54:10 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 3 Apr 2000 13:33:48 +0100 (GMT Daylight Time)
Subject: Re: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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SEE
http://www.anglepoise.co.uk/



Date sent: Sun, 2 Apr 2000 21:34:13 -0400 (EDT)
} From: donald j marshall {dmrelion-at-world.std.com}
To: KPR-at-wpo.nerc.ac.uk


I heard this one at a conference last week. A PhD student
was caught sawing a knob off a FE SEM. Apparently it was
pushing against his desk so decided to saw it off and was
going to glue it on at a 45 degree angle!

Dave




On Sat, 1 Apr 2000 06:54:53 -0000 Lou Solebello
{microls1297-at-mindspring.com} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} My story is not as much of a horror as it is embarassing. Several years
} back I received a package of samples from a regular client without any paper
} work describing what the samples were. This is not unusual since we
} frequently recieve unknowns. I proceeded to open the package and was
} abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
} of the samples was a vial of concentrated bannana flavoring. It was months
} (almost a year) before the odor completely disappeared from my office, and I
} was cajoled about it frequently. I by the way, hate bannanas.............
}
} Lou Solebello
} -----Original Message-----
} } From: L R MELSEN {lmelsen-at-emory.edu}
} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
} Date: Friday, March 31, 2000 9:26 PM
} Subject: Storys
}
}
} } ------------------------------------------------------------------------
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} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } We had a cousin of Conan , who in his addled age, after inserting the
} } injector tip into the stage of our EM400 could not find his grid in the
} } microscope; he had dropped it on the floor. Of course the tip must have
} } fallen off in the stage, so he promptly took a second tip and properly
} } inserted the second tip on top of the first. Still no grid could be
} } found. Ah ha, I will leave a polite note explaining the problem. It read
} } as follows:
} } " Please check the microscope, I had great difficulty inserting my
} } specimens last evening."
} } Philips kindly replaced the bulk of the stage just so they could keep
} } the original for the museum of what not to do.
} }
} }
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Apr 03 08:09:35 2000



From: Julian Smith III :      smithj-at-Winthrop.edu
Date: Mon, 3 Apr 2000 07:56:46 -0500
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
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It does't have oxygen in it--with air, 02- is produced and accelerated at
your specimens, eroding them. When done intentionally, this is called
"plasma ashing," i believe. If your specimens look very smooth, this may
be why.
Argon produces Ar2+, which, if your coater is set up right, is accelerated
at the gold target.
JSIII

} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90


Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)




From daemon Mon Apr 03 17:47:39 2000



From: Van Osta, Peter [JanBe] :      PVOSTA-at-janbe.jnj.com
Date: Mon, 3 Apr 2000 15:50:19 +0200
Subject: Illumination Technologies lightsource to ZEISS micrscope

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Has anyone foud a solution for the following problem, I am getting
desperate. There doesn't seem to be a ready-made solution available ?

We want to link an Illumination Technologies light-source to a ZEISS
microscope. We are trying to get the right information about the necessary
parts, but this seems to be non-trivial.

The following problems need a solution:

ZEISS Axioskop upright microscope and Illumination Technologies CF1000
ZEISS Axiovert 100M inverted microscope and Illumination Technologies 3900

In both cases we need a light guide and a fiber coupler to the microscope.
Both light sources are to be used for epifluorescence applications.

Sincerely yours,

Peter Van Osta, MD

Senior Scientist Medical Image Analysis
Biological Imaging Laboratory
Life Sciences Department I - 6065
Janssen Research Foundation
Turnhoutseweg 30
B-2340 Beerse
Belgium
Europe

tel.: +32 (0)14 60.60.65 (office hours, GMT+1)
fax.: +32 (0)14 60.57.88

email: pvosta-at-janbe.jnj.com

WWW: http://ourworld.compuserve.com/homepages/pvosta




From daemon Mon Apr 03 17:47:40 2000



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Mon, 3 Apr 2000 10:22:20 -0400
Subject: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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I heard this one from the Philip's engineers: A brand new Philips
microscope was being installed in a goverment laboratory. It was to be in
a state of the art, brand-new building. Everything was there, house
nitrogen, chilled water, etc. Even the darkroom was state of the art.
Well, when the in-house plumbers hooked up the water to the microscope they
weren't being very careful in their reading of the blueprint designs and
they had D-19 developer running through the EM instead of water. I'm not
sure, but I think they got a new microscope out of that blunder!


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com







From daemon Mon Apr 03 17:47:43 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Friday, March 31, 2000
Subject: Fwd: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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An additional story which had the potential of being a real personnal horror story is as follows:

I worked for Humberto Fernandez-Moran at the University of Chicao many years ago. For those of you who are not familiar with the name, he was instrumental in developing the first diamond knives, producing the first pointed filaments for routine use and construction of first cryo-TEM using liquid helium cooled lenses. It was a very interesting place for a young budding microscopist at the time!

Dr. Moran had a large scar on his nose. He said it was from the removal of a cancerous skin area. He claimed to have gotten the malignancy in that location due to using electron microscopes in the late 40's without the benefit of lead glass windows. They used to press their noses against the window when concentrating on the relatively dim image projected by those early instruments. I wonder if other early EM researchers eventually developed cancer which might be related to similar research experiences.

As it turned out, Dr. Moran lived a long life, although not without controversy through the years. He had a very unusual life history and was also a brilliant but erratic person to interact with....somewhat akin to what Bobby Knight is to basketball!

Debby
--------------------------------------


Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057







From daemon Mon Apr 03 17:47:44 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Mon, 03 Apr 2000 09:10:59 -0400
Subject: UHV compatibility of phenolic resins

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I have just been asked to do auger analysis on a sample mounted in phenolic
mounting resin. Normally I require that these samples be demounted for UHV
compatibility but this person doesn't wish to do so.

Does anyone have any experience with putting phenolic mounting material in
a UHV environment? Will I be seeing carbon on all my samples for the next
few years?

Thanks,
Henk

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Murphy's Law: "If anything can go wrong, it will."
Commentary: "Murphy was an optimist."



From daemon Mon Apr 03 17:47:45 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 03 Apr 2000 12:22:45 -0700
Subject: Re: Illumination Technologies lightsource to ZEISS micrscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Peter,

There is a new system which sounds like it a good fit for your application.
It is a liquid light guide with coupler, lamp (long lived HBO) and power
supply from EFOS. Contact: Allan Firhoj PH: 905-812-4302 Email:
AllanF-at-EFOS.com URL: www.efos.com

Caveat: MME has no financial interest in this product.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 03:50 PM 4/3/00 +0200, Van Osta, Peter [JanBe] wrote:
} ------------------------------------------------------------------------
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From daemon Mon Apr 03 17:47:47 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 3 Apr 2000 13:15:39 -0500
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I love reading the "horror stories". Here's a personal one:

When I was a grad student I did a CPD run with a fellow grad student JT. I
worked on plant pathogens and JT worked on chick embryo hearts so we had
little pieces of leaf tissue and tiny chick hearts to dry. When the run was
finished I couldn't get the lid off the CPD (it was a twist type). JT
volunteered to muscle it off and when she did the lid shot passed her head
with a boom and hit the ceiling. She had a grazing wound on her forehead
but was otherwise ok. We both burst into fits of nervous laughter...we both
knew she was so lucky not to be seriously injured. Then we looked in the
CPD and saw that all the lids had blown off the little white sample
containers. We howled with laughter when we got down on our hands and knees
to search the floor for the tiny hearts and leaf pieces. The CPD was fine,
the samples were fine, and surprisingly we both graduated.

Beth




From daemon Mon Apr 03 17:47:50 2000



From: Gerald Harrison :      jerry-at-biochem.dental.upenn.edu
Date: Mon, 03 Apr 2000 14:04:03 -0400
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello to Beth and all,

} [Beth wrote:]
} I love reading the "horror stories". Here's a personal one:

I do, also. Although I have not posted to this List often (last
time was for advice in purchasing a confocal which was very helpful and we
are very pleased with the purchase), I've wanted to chime in and say how
enjoyable it is to read these 'stories'.
I think there is a very relevant component to these stories in
that they help us be more aware of how easy it is for some very unusual,
silly and sometimes dangerous things to happen when you thought such a
thing was impossible if it even crossed your mind at all.

Sorry, but thankfully, (very thankfully) I have no horror stories,
yet... Having said that, uh oh...

Gerald Harrison
================
} [Beth continued]
} When I was a grad student I did a CPD run with a fellow grad student JT. I
} worked on plant pathogens and JT worked on chick embryo hearts so we had
} little pieces of leaf tissue and tiny chick hearts to dry. When the run was
} finished I couldn't get the lid off the CPD (it was a twist type). JT
} volunteered to muscle it off and when she did the lid shot passed her head
} with a boom and hit the ceiling. She had a grazing wound on her forehead
} but was otherwise ok. We both burst into fits of nervous laughter...we both
} knew she was so lucky not to be seriously injured. Then we looked in the
} CPD and saw that all the lids had blown off the little white sample
} containers. We howled with laughter when we got down on our hands and knees
} to search the floor for the tiny hearts and leaf pieces. The CPD was fine,
} the samples were fine, and surprisingly we both graduated.
}
} Beth
}




From daemon Mon Apr 03 17:47:51 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Mon, 3 Apr 2000 14:12:21 -0400
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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I can't even begin to think of why you would have D-19 developer in a
pressurized pipe system? Did they process 1000's of negatives a day?


At 10:22 AM -0400 4/3/00, Peggy Bisher wrote:
} ------------------------------------------------------------------------
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David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Mon Apr 03 17:47:51 2000



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Mon, 3 Apr 2000 14:40:34 -0400
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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The Philip's guy's did not give me such a clear answer. I can only
assume that something stupid happened, like D-19 was put in the closed loop
system of the Haskris Chiller, instead of the normal water. I do know that
this darkroom was to service a large EM suite so perhaps there was this big
tank of D-19 made up and they (the plumbers) grabbed it and used it. It
does sound incredibly stupid, that's for sure.


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Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com







From daemon Mon Apr 03 17:47:53 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 3 Apr 2000 08:59:07 -1000 (HST)
Subject: Microscopy horror stories

Contents Retrieved from Microscopy Listserver Archives
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These stories are a giggle, and also food for thought!

I would like to collect them for a future Net Notes, part of the News and
Commentary section of Microscopy and Microanalysis. So keep them
coming! I may have to edit them down to a reasonable number, and it would
be a few months before they appeared, but they are too good to pass up.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Apr 03 17:47:57 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 03 Apr 2000 16:24:06 -0400
Subject: Re: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lou Ann Miller wrote:

} We had a CAP inspection, the officers were from another hospital in the state.
}
}
} The question I was expected to reason and answer with a straight face was:
}
}
} * What other protective measures besides standing behind a lead
} wall do you take when you put the glass slide into the TEM, to
} protect yourself from the flying electrons???
}

Dear Lou Ann,
In addition to the obvious ludicrous aspects to the question, the
use of a lead shield to protect oneself against "flying electrons" is one
of the worst things to do. One should use a low-Z absorber for elec-
trons to reduce brehmsstrahlung x-rays, then, if desired, put lead
between oneself and the low-Z shield to absorb those x-rays which
are produced.
Yours,
Bill Tivol



From daemon Mon Apr 03 17:47:58 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 03 Apr 2000 16:35:30 -0400
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Damian wrote:

} I heard that one years ago. Also one that supposedly happened at Univ.
} Wisconsin. Someone apparently cleaned a scope's internal parts in acetone
} and started pumping. Before it was fully pumped down, they turned on the
} filament. Bang! Nice story but could it possibly happen? I would think
} that any acetone residue would evaporate very quickly or is there some
} other phenomenon here relating to ratio of gases?

Dear Damian,
There must have been a lot of residue in the scope to have caused

an explosion. Not only would one need both acetone and oxygen in a
suitable ratio, there would have to be enough so that the heat of combustion
from one acetone molecule oxidising would cause other molecules to
react, otherwise there would just (!) be a slow burning of the vapors. In
addition, the acetone/oxygen ratio would be determined by both the
amount of residual acetone and the relative pumping speeds for acetone
and oxygen. No doubt there are people on this list who would know
the pump speed ratio, so one could calculate the expected behavior of
the acetone in the column for various values of the relevant parameters.
Yours,
Bill Tivol




From daemon Mon Apr 03 17:48:00 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 3 Apr 2000 17:43:21 EDT
Subject: Re: Microscopy horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If no one has any objections I would also like to post these on our upcoming
section on microscopy for our museum's web site when it is finished.
Ed Sharpe archivist for SMECC



From daemon Mon Apr 03 17:48:01 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Mon, 3 Apr 2000 16:45:46 -0500
Subject: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't mean to be a drag, but enough of these "horror stories". My email
is clogged with these stories. I only want the facts, Sir. Thanks.



From daemon Mon Apr 03 17:48:02 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Mon, 3 Apr 2000 18:09:49 -0400
Subject: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I remember somewhere in the past a discussion of software for
creating extended focus images (I think that is the correct term), in
which a series of transmitted light images in different focal planes
are combined in order to remove the out of focus information and show
all parts of the image in focus. Can someone point to software that
carries out this function? Thanks- Dave
--

************************************************************
"Home of the 2000 NCAA Women's Basketball Champions"

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Mon Apr 03 18:28:05 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Mon, 3 Apr 2000 18:10:46 -0500
Subject: SEM: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've just noticed behavior which is different for my 2 e-beam
instruments. If I bias my microprobe's gun for less emission, the
beam current measured in a specimen faraday cup also goes down.
However, if I bias my SEM's gun for less emission, the beam current
(measured similarly) goes up(???). This may mean simply a shallower
optic angle for the SEM and the anode allowing more electrons to pass,
but I thought I'd throw the observation out there.

cheerios, shAf

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - ICQ 210524
Geological Science's Electron Probe Facility - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/




From daemon Mon Apr 03 18:28:05 2000



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Mon, 3 Apr 2000 18:09:33 -0500
Subject: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi everyone!
I'm new to this microscopy society and 'am having trouble already!!To make
it worse everyone is sending me panic stories!! Just kidding!
I'm working on some poly-propylene wires. I need to take some TEM images but
I'm having trouble at the first stage itself...ultramicrotomy. You see I
need to fuse two PP wires and image the interface. Now the problem is, when
I'm cutting it with the microtome, the interface just breaks off!!Right now
I'm using a epoxy-hardner ratio of 10:4. will changing the proportions help?
I'm thinking of using a diamond knife also. Does anyone have any bright
ideas?!!Help me out on this!!
Praveena
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com




From daemon Mon Apr 03 18:58:17 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 3 Apr 2000 19:49:27 EDT
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 4/3/00 6:23:17 PM, knecht-at-uconnvm.uconn.edu writes:

} I remember somewhere in the past a discussion of software for
} creating extended focus images (I think that is the correct term), in
} which a series of transmitted light images in different focal planes
} are combined in order to remove the out of focus information and show
} all parts of the image in focus. Can someone point to software that
} carries out this function? Thanks- Dave

That function is performed in The Image Processing Tool Kit
(http://members.aol.com/ImagProcTK/) by examining each pixel location in the
two (or more) images and calculating the local variance in a 5 pixel wide
circle. The pixel value that has the largest variance is kept, the rationale
being that it has the most abrupt local variations and is likely to be in the
sharpest focus. The method works pretty well for most microscope images where
the magnification remains constant, but not so well for macroscopic images
where the out-of-focus portions of the image are also shifted due to changes
in focal length. It also doesn't handle cases with specular reflections to
well. But it should be OK for your transmitted light case. There are other
approaches in some software packages that use a simple high pass filter (e.g.
a Laplacian) but this seems from my experiments to be no faster and more
noise sensitive.



From daemon Mon Apr 03 19:51:34 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Mon, 3 Apr 2000 17:23:13 -0700
Subject: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lighten up - the delete button is easy enough to use :-) I often feel the
same way about all the bio postings and these are a lot more fun to read,
besides having some educational value.

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: Kriho, Virginia [mailto:Vkriho-at-psych.uic.edu]
Sent: Monday, April 03, 2000 2:46 PM
To: 'List Server'


I don't mean to be a drag, but enough of these "horror stories". My email
is clogged with these stories. I only want the facts, Sir. Thanks.



From daemon Mon Apr 03 19:51:34 2000



From: Harrison :      tuttle-at-home.com
Date: Mon, 3 Apr 2000 17:31:00 -0700
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's another one. A customer on the west coast had a bottle of N2 that
was used to vent their TEM . They also used the same bottle to agitate their
developer. One day someone left the N2 on in the developer and it ran out.
You can guess what happened next, the TEM was vented with D-19.

Dave Harrison




From daemon Mon Apr 03 21:46:52 2000



From: Don Grimes :      microtoday-at-mindspring.com
Date: Mon, 3 Apr 2000 22:30:15 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,
Just a note to advise that I intend to publish a summary of these "horror"
stories in an upcoming issue of Microscopy Today. For those of you who do
not receive our publication, including those overseas, I will provide a copy
of the summary - at no charge.
To the authors (past, current and future), should you not wish to have your
comment included on our summary, kindly advise by return email and I will
insure that it does not happen.
Best to all,
Don Grimes, Microscopy Today



From daemon Mon Apr 03 22:26:58 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 04 Apr 2000 13:18:40 +1000
Subject: Re: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all -
Ditto for the Australian EM Newsletter. (Somebody has volunteered to put
together a choice selection.).
and one from us...
-the guy who asked how long to wash between dehydration steps

and from another place a long time ago-
-the Professor who offered to pay the electron microscopist by giving him
some oil immersion objectives for the TEM.








Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525

} } } "Don Grimes" {microtoday-at-mindspring.com} 04/04/00 01:30pm } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Group,
Just a note to advise that I intend to publish a summary of these "horror"
stories in an upcoming issue of Microscopy Today. For those of you who do
not receive our publication, including those overseas, I will provide a
copy
of the summary - at no charge.
To the authors (past, current and future), should you not wish to have
your
comment included on our summary, kindly advise by return email and I will
insure that it does not happen.
Best to all,
Don Grimes, Microscopy Today




From daemon Mon Apr 03 22:27:00 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Mon, 3 Apr 2000 20:19:04 -0700
Subject: Re: Horror Stories rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These stories are fun, but we are spending a lot e-mail on them.

I suggest these rules:
1. The story should have happened to you or your lab.
Don't repeat "Urban Legends" like "acetone vapor explodes microscope".

2. Don't comment on the stories. Assume everyone understands the problem or
science behind the story.

Now I will duck behind the computer.

Ron Vane
XEI Scientific



-



From daemon Mon Apr 03 22:50:50 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 03 Apr 2000 20:38:22 -0500
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know that Soft Imaging's AnalySIS with EFI does this. It takes a bunch
of images that are focused at various points and combines them into one
image which is totally in focus.

gary g.



At 05:09 PM 4/3/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I remember somewhere in the past a discussion of software for creating extended focus images (I think that is the correct term), in which a series of transmitted light images in different focal planes are combined in order to remove the out of focus information and show all parts of the image in focus. Can someone point to software that carries out this function? Thanks- Dave
} --
}
} ************************************************************
} "Home of the 2000 NCAA Women's Basketball Champions"
}
} Dr. David Knecht
} Department of Molecular and Cell Biology
} University of Connecticut
} 75 N. Eagleville Rd. U-125
} Storrs, CT 06269
} Knecht-at-uconnvm.uconn.edu
} 860-486-2200 860-486-4331 (fax)
} home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
} ************************************************************



From daemon Tue Apr 04 15:00:05 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 03 Apr 2000 21:54:53 -0700
Subject: RE: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I strongly believe that many of us are able to extract facts even from
"horror stories". Fact, what is that? In my point of view, the "horror
stories" are pure extract from people's experience showing to us how manage
EM facilities properly. I was surprised to know, for instance, that
somebody was able to bent sample-holder standing up from the chair. I will
include special topic about that case in my instructions for users now. If
you don't like the way people share their experience, you may set filter tn
the word "horror" in the E.mail program and direct those messages into the
trash-folder. A little bit humor, here at ListServer is not bad.

Best wishes, Sergey.

P.S. It is not bad tradition, again, at ListServer to sign the messages.

} Date: Mon, 03 Apr 2000 16:45:46 -0500
} From: "Kriho, Virginia" {Vkriho-at-psych.uic.edu}
} Subject: no more horror stories
} To: 'List Server' {microscopy-at-sparc5.microscopy.com}
} X-Mailer: Internet Mail Service (5.5.2448.0)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Tue Apr 04 15:00:07 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Tue, 04 Apr 2000 15:41:31 +1000
Subject: Lessons in horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been responsible for a multi-user facility since 1970.

It has always run on the basis that anyone can walk in and learn to use the
equipment. So we never have fights about access or possession.

We now have around 300 users of which about 150 start fresh each year.

How is it our machines are not all wrecks?

The FIRST lesson teaches two golden rules:

1. NEVER use force on any control

2. ALWAYS ask for help as soon as you dont understand what is happening.

Our few bad incidents have occurred because these rules were neglected.

When a user who is in trouble calls me in to sort it out I try very hard to
always be cheerful and positive no matter how stupid they have been. I
think if I am cheerful they will call me in next time they have a problem.
If I am furious with them they will maybe try to hide their blunder, or
worse, try to fix it themselves.

Of course, I must apply the rule to myself. When I dont understand what is
happening, it is time to call the service engineer!


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Tue Apr 04 15:00:08 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 4 Apr 2000 16:19:33 +1000
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, after reading Peggy Bisher's story, I couldn't help but add another
along similar lines.

About 20-odd years ago, a prestigous institution purchased a state of the
art new TEM. The main body came in a very large wooden container and was
unloaded onto the loading dock at the back of the building. It sat there
for quite a while, because it was too heavy to move with the regular
forklift, and I think the lab still needed a few final things to be
finished off. Anyway, one day, someone decided that they were going to
move the TEM in, and loaded it on the forklift. About half way to the lab,
the TEM started oscillating back and forth on the forklift - it wasn't
strapped on securely, and an eyewitness said he just stood there and
watched this thing slowly crash to the floor on its side. Not much use
rushing in and getting crushed by a few 100 kilos of metal.

I'm not sure it ever worked properly, the camera was smashed and a few
other things too. The workshop had to retool all the smashed bits as best
they could.

Amazing how many ways there are to destroy precision instruments.

cheers,

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 04 15:00:21 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 4 Apr 2000 09:39:37 +0100 (BST)
Subject: preventing horrors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I agree with Mel Dickson whole heartedly. I learnt many years ago
that to bawl out a user for being stupid results in silence. The only way
to find out what really happens is to be as helpful as possible whatever
the situation. Rant and rave to let off steam later, in the privacy of
another room.
To this end I welcome the horror stories. It is better to tell
users stories of other incidents to get them to be careful and to
think about the situation. They are more willing to ask a `stupid'
question without embarrasment if they are aware of the mistakes that have
been made. If this prevents them making furhter mistakes then I'm all for
it.

Ron

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================





From daemon Tue Apr 04 15:00:22 2000



From: John Runions :      cjr41-at-cam.ac.uk
Date: Tue, 04 Apr 2000 09:39:30 +0100
Subject: Re: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What Jan is saying is that `"bio" postings have no educational value ; )

Markham Jan-AFP042 wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Lighten up - the delete button is easy enough to use :-) I often feel the
} same way about all the bio postings and these are a lot more fun to read,
} besides having some educational value.
}
} Jan Markham
} Motorola FPDD
} 7700 South River Parkway, FPD22
} Tempe, AZ 85284
} Ph: (480)755-5509
} FAX: (480)755-5115
} Email: afp042-at-email.mot.com
}
} -----Original Message-----
} } From: Kriho, Virginia [mailto:Vkriho-at-psych.uic.edu]
} Sent: Monday, April 03, 2000 2:46 PM
} To: 'List Server'
} Subject: no more horror stories
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I don't mean to be a drag, but enough of these "horror stories". My email
} is clogged with these stories. I only want the facts, Sir. Thanks.

--
C. John Runions, Ph. D.
Department of Plant Sciences
University of Cambridge
Downing St.
Cambridge
UK

email cjr41-at-cam.ac.uk
phone (01223) 529 249




From daemon Tue Apr 04 15:00:23 2000



From: Arnold Pizzey :      a.pizzey-at-ucl.ac.uk
Date: Tue, 04 Apr 2000 09:51:50 +0100
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} I remember somewhere in the past a discussion of software for
} creating extended focus images (I think that is the correct term), in
} which a series of transmitted light images in different focal planes
} are combined in order to remove the out of focus information and show
} all parts of the image in focus. Can someone point to software that
} carries out this function? Thanks- Dave
} --
}
}
Greetings David,

Try Auto-montage (http://www.synbiosis.com/syncroscopy/am.asp)

If you contact them they will send you a demonstrtion CD of this product.

regards

Arnold

_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
Arnold Richard Pizzey
Department of Haematology
Royal Free and University College London Medical School
98 Chenies Mews
London WC1E 6HX
U.K

voice: +44 0207-209-6234
Fax: +44 0207-209-6222
email: a.pizzey-at-ucl.ac.uk
_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/



From daemon Tue Apr 04 15:00:35 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 4 Apr 2000 19:45:20 +1000
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dave,

There are a couple of options. Autoquant will do this, as will modules
from Soft Imaging Systems. I think some Zeiss software can do this too, as
long as there is no lateral shift in sequential images (as is found in some
dissecting scopes). These are packages we've tried, there may well be more.

cheers,
Rsoemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 04 15:00:37 2000



From: TQ23TEAM-at-LHT.DLH.DE
Date: Tue, 4 Apr 2000 11:55:12 +0200
Subject: AW: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dave,
you may have a look on the SIS (Soft Imaging Software) homepage. They offer
an EFI -modul.

http://www.soft-imaging.de/

In our metallographic labority, we use the EFI-module for one year and we
are very satisfied in working with it.

Hope ths answer will help.

Best regards
Bernd Schweisfurth
Lufthansa Technik
Hamburg / Germany
} ----------
} Von: David Knecht[SMTP:knecht-at-uconnvm.uconn.edu]
} Gesendet: Dienstag, 4. April 2000 00:09
} An: microscopy-at-sparc5.microscopy.com
} Betreff: extended focus software
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I remember somewhere in the past a discussion of software for
} creating extended focus images (I think that is the correct term), in
} which a series of transmitted light images in different focal planes
} are combined in order to remove the out of focus information and show
} all parts of the image in focus. Can someone point to software that
} carries out this function? Thanks- Dave
} --
}
} ************************************************************
} "Home of the 2000 NCAA Women's Basketball Champions"
}
} Dr. David Knecht
} Department of Molecular and Cell Biology
} University of Connecticut
} 75 N. Eagleville Rd. U-125
} Storrs, CT 06269
} Knecht-at-uconnvm.uconn.edu
} 860-486-2200 860-486-4331 (fax)
} home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
} ************************************************************
}



From daemon Tue Apr 04 15:00:39 2000



From: Molecular Biology Lab - Dept of Botany :      paam-at-rs.uovs.ac.za
Date: Tue, 4 Apr 2000 12:42:15 GMT2
Subject: Immunogold labeling background

Contents Retrieved from Microscopy Listserver Archives
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I am trying to localize a beta-1,3-glucanases gene expression after Russian
wheat aphid infestation. However I'm getting heavy labeling in the chloroplasts.
Unexpected because no previous localization studies have shown glucanases
to be expressed in the chloroplasts. My question is: How can I conform that
the labeling I am getting is not background or artifacts of some sort??
I am not using osmium only uranyl acetate and lead citrate for the staining

Thank you for any assistance
Martin Wilding


Martin Wilding
Department Botany & Genetics
University of the Orange Free State
P.O. Box 339
Bloemfontein
9300
South Africa

Tel +2751 4012818
Fax +2751 4488772
Email paam-at-rs.uovs.ac.za



From daemon Tue Apr 04 15:00:40 2000



From: Everett Ramer :      Everett.Ramer-at-netl.doe.gov
Date: Tue, 04 Apr 2000 07:06:54 -0400
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
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David,
I wrote a macro for Optimas that does this; however, you should be able to do this with just about any image processing software. You can see an article I wrote in Microscopy Today, February/March 1988 for specifics. I can email you a copy of the article if you want it.


Everett Ramer
National Energy Technology Laboratory
Pittsburgh, PA, USA
412-386-4920
ramer-at-netl.doe.gov

} } } David Knecht {knecht-at-uconnvm.uconn.edu} 04/03/00 06:09PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I remember somewhere in the past a discussion of software for
creating extended focus images (I think that is the correct term), in
which a series of transmitted light images in different focal planes
are combined in order to remove the out of focus information and show
all parts of the image in focus. Can someone point to software that
carries out this function? Thanks- Dave
--

************************************************************
"Home of the 2000 NCAA Women's Basketball Champions"

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Tue Apr 04 15:00:44 2000



From: Don Grimes :      microtoday-at-mindspring.com
Date: Tue, 4 Apr 2000 08:07:30 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Group,
As suggested by several of you, and in addition to publishing an edited
summary of the stories in Microscopy Today, I will put the summary on my web
site where it can be downloaded by any with an interest. I will advise when
it is done.
And, should Nestor decide to discourage further contributions, send comments
to me directly and I will see that they are included.
Regards to all,
Don Grimes, Microscopy Today



From daemon Tue Apr 04 15:00:49 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Tue, 4 Apr 2000 09:16:28 -0400
Subject: RE: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the problems with many interfaces like yours, Praveena, is that there
is not a lot of adhesion across them. Thus mechanical sectioning of any
form places a stress on the interface and decohesion can occur. (In many
cases of sectioned thin films at this lab, we've ended up with two debonded
layers adhering to the epoxy but not to each other after sectioning, thus I
don't think playing with the hardener ratio will help much). A diamond
knife makes a much smoother 'cut' and thus reduces these stresses, as will a
lower knife angle (like 35 degrees). Sectioning parallel to an interface is
less stressful than perpendicular to it. If you have an inherently weak
interface, however, chances are pretty good that decohesion will occur. You
may increase your probability of finding a portion that is still together by
trimming to an oversized block face.

Good luck.

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca
----------
From: Praveena Bhaskara [SMTP:bubbyp-at-hotmail.com]
Sent: April 03, 2000 7:10 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: ultramicrotomy: PP wires


------------------------------------------------------------------------
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-----------------------------------------------------------------------.



Hi everyone!
I'm new to this microscopy society and 'am having trouble
already!!To make
it worse everyone is sending me panic stories!! Just kidding!
I'm working on some poly-propylene wires. I need to take some TEM
images but
I'm having trouble at the first stage itself...ultramicrotomy. You
see I
need to fuse two PP wires and image the interface. Now the problem
is, when
I'm cutting it with the microtome, the interface just breaks
off!!Right now
I'm using a epoxy-hardner ratio of 10:4. will changing the
proportions help?
I'm thinking of using a diamond knife also. Does anyone have any
bright
ideas?!!Help me out on this!!
Praveena
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com





From daemon Tue Apr 04 15:00:49 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 4 Apr 2000 09:25:21 -0400 (EDT)
Subject: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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This is less a horror story than a sad tale. When I took over managing
the EM facility here, I ordered a new cylinder of CO2 for the CPD. When
it arrived, I pointed out to the delivery person that he made a mistake
and had not supplied a tank with a siphon tube. He replied that he
delivered what he had always delivered. I checked the shipping records,
and, indeed, my predecessor had used C02 from a tank without a siphon
tube--in other words, for a decade he never critically point dried
a single specimen, since only C02 gas would have entered the chamber!

Perhaps more than a few people may want check their cylinders. (In the
US, the proper cylinder typically has a red band painted at the top of the
cylinder and the words "w/ dip tube" stenciled on the side.)

DL

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718







From daemon Tue Apr 04 15:00:50 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Tue, 4 Apr 2000 15:37:29 +0000
Subject: Operator Horror Story

Contents Retrieved from Microscopy Listserver Archives
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Hi all:
Many years ago when comunists were in power in Eastern Europe, thanks
to a great person who knew how to deal with them, we got a beautiful
pice of equipment, with all the possible stages. One of them - the
heating stage - was especially impressive. All the people were amazed
that in situ TEM heating experiment might be performed up to 1000
centigrade. Among them was a young scientist who was investigating
some processes in aluminum. Probably, impressed by 1000 centigrade he
forgot about melting temperature. So, the new stage was required. At
this time it was a real horror story since the price of the stage was
almost equal the price of the small car - Fiat 126.
Have a nice day,
Witold Z.







%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Tue Apr 04 15:00:51 2000



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Tue, 4 Apr 2000 09:51:02 -0400
Subject: Correction: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Hi George,

You didn't read my tale too carefully. I said the "in-house plumbers" did
the error. The Philip's folks had nothing to do with this blunder. They
just told me the story because it involved one of their microscopes. Maybe
I should have just left out who told me the story. I was only trying to
give them the credit and not take it myself. I am sure all of our EM
service engineers have some horror stories that would top any we have told,
but are trying to be polite for all of our sakes.

}
} Peggy told one on the Philips folks:
}
} } ... new Philips microscope ... goverment laboratory. ... house
} } nitrogen, chilled water, etc. Even the darkroom was state of the
} } art. Well, when the in-house plumbers hooked up the water to the
} } microscope they weren't being very careful in their reading of the
} } blueprint designs and they had D-19 developer running through the
} } EM instead of water. I'm not sure, but I think they got a new
} } microscope out of that blunder!
}


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com







From daemon Tue Apr 04 15:00:52 2000



From: William Carmichael :      wcarmichael-at-madison.tec.wi.us
Date: Tue, 04 Apr 2000 08:51:58 -0500
Subject: Re. extended focus software

Contents Retrieved from Microscopy Listserver Archives
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We use the AnalySIS software from Soft Imaging Systems in our lab and it works great. It's very easy to use and the results are an image which is in focus from top to bottom.



_______________________________

Bill Carmichael
Electron Microscopy Faculty

Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309

wcarmichael-at-madison.tec.wi.us
http://electron-microscopy.madison.tec.wi.us




From daemon Tue Apr 04 15:00:53 2000



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Tue, 04 Apr 2000 09:57:13 -0400
Subject: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Praveena wrote:

Hi everyone!
I'm new to this microscopy society and 'am having trouble already!!To make
it worse everyone is sending me panic stories!! Just kidding!
I'm working on some poly-propylene wires. I need to take some TEM images but
I'm having trouble at the first stage itself...ultramicrotomy. You see I
need to fuse two PP wires and image the interface. Now the problem is, when
I'm cutting it with the microtome, the interface just breaks off!!Right now
I'm using a epoxy-hardner ratio of 10:4. will changing the proportions help?
I'm thinking of using a diamond knife also. Does anyone have any bright
ideas?!!Help me out on this!!
Praveena



I am not sure I understand the "fusing" part of your question. However PP
has a Tg of -19 so you need cryo-microtome, if you are not perhaps that is
part of the problem. By the way, hopefully these "panic" stories will not
go on much longer. They are not usual to the list. Steve
Stephen McCartney
Research Associate
Virginia Tech
Materials Institute
2108 Hahn Hall
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Tue Apr 04 15:00:54 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 09:07:19 -0700
Subject: Horrors in the field

Contents Retrieved from Microscopy Listserver Archives
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To All,
As a field engineer, I fully agree with both Mel and Ron and have always
told my customers, "The only stupid question is the one that you don't
ask."

I will also say that occassionally it can be very difficult to hold
one's temper when someone has done something stupid on an instrument
covered by one's own service contract and then they try to lie about
it. Users, don't add insult to injury. Tell us what you did so that we
can more rapidly repair the damage by looking in the right direction.

Field engineers have horror stories, to:

I became an expert on the ETEC Autospec WDS as an ETEC field engineer by
being impatient in looking for vacuum leaks. The Autospec about doubles
the volume of the system and therefore takes a lot longer to vent. I
had put a 13-1/2 stopper in the port for the secondary detector to see
if the SED was the source of the leak. When it was about half vented,
I pulled the stopper. Without the WDS, this wouldn't have caused any
problem, but I drove the columnator/electron trap into the 4 crystal
turret, broke the tape drive and blew the thin window detector, along
with destroying 2 crystals and loosening all 4. The process of fixing
all this was a three week intensive course in WDS alignment and
operation.

The moral: Don't rush. Take it easy and (God forbid) THINK before you
act. It was only milliseconds to create 3 weeks of work and some $4k in
damaged parts.

Moral #2: Learn from the mistakes of others, because you'll never live
long enough to make them all yourself.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:00:56 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 09:14:57 -0700
Subject: MAC drawings

Contents Retrieved from Microscopy Listserver Archives
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Are there any MACs out there?,

I have the original drawings for MAC instruments that ETEC dumped. I
can't vouch for completeness, but there are 4 boxes of tubes with B size
drawings and larger and at least a ream of A size drawings.

I need room. If you need these drawings, please contact me before May
1. After that date I will dump them. They may be had for the cost of
shipping.


Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:00:57 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 09:19:34 -0700
Subject: ETEC Omniscan parts

Contents Retrieved from Microscopy Listserver Archives
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Omniscan users,

Are there any of you left out there? I am trying to decide what to do
with all of the parts aquired from ETEC. If you are still using your
Omniscan, please let me know as I don't want to leave people stranded,
but I could really use the space if there is no need for these parts.


Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:01:04 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 10:30:53 -0700
Subject: Re: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
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O.K. I guess I didn't word that as well as I should have :-) However, my
point remains valid - not all the postings are of interest to everyone, no
matter what the content, and these have been fun.

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: John Runions [mailto:cjr41-at-cam.ac.uk]
Sent: Tuesday, April 04, 2000 1:40 AM
To: Markham Jan-AFP042
Cc: 'Kriho, Virginia'; 'List Server'


Garber, Charles A. wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Here is a "just off the press" example (but this does come up at least once
} a year):
}
} I fielded a phone call from a distraught SEM lab manager who told me that
} some months ago he put Dow Corning fluid into his diffusion pump "to save
} money". And now that he has had an accident, the silicone fluid of course,
} has contaminated his system, so he was asking us, "what organic solvent will
} easily remove it."
}
} He was especially upset when I told him that his EDS detector will see Si
} everywhere also, because they do a lot of analyses for Si!
}
} I won't repeat what he told me when I tried to explain the reality of his
} situation......
}
} But it does go to show that there are a lot of new people entering our
} profession, some with less training and experience with vacuum than others,
} so such stories are very well worth repeating.
}
} But just out of curiosity, is there some "recommended procedure" for
} removing silicone fluid from the internal parts of a column, and also
} removing it from the window of an EDS detector? I presume one can always
} call in an outside service provider with experienced people but a lot of
} users out there just don't have the budget for something like that. But
} they do have a good supply of student manpower.
}
} While we are on the subject of silicone, a few weeks ago a well known TEM
} user got me on the phone to say "hello" and commented that he had just
} placed an order for silicone grease and yes, he said, he was going to be
} using it on the o rings of his column. I told him I thought that he should
} be using other greases and his response was "don't listen to dogma, I
} thought you read the listserver!". Am I correct, namely that one should not
} be using silicone grease on the o rings of a column instrument?
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
Chuck,
I agree that he shouldn't be using silicone grease on his o-rings.
Braycote 803 and 602 are what I use, for static and dynamic seals,
respectively.

My experience with DC705 (Transene Vacoil-S), which all ETECs were
shipped with, is that if the system is properly trapped and the vacuum
logic is well thought-out, it seems to work just fine. The only user
that I'm aware of that had problems with stray Si readings was one who
had a SIMS system attached. SIMS is apparently very sensitive to Si.

If you burp your DP, it is diffcult to clean, although ispropanol seems
to work fairly well. I've seen many burped systems, but have never had
any latent problems with silicates causing excessive charging. The
biggest plus of DC705 is that you can take it to atmosphere hot and the
oil is indestructible. It's performance figures are quite acceptable,
and, lets face it, most people don't think twice about what they stick
in their vacuum system. A bullet-proof oil is nice to have.

Perhaps it's more of a problem on systems that don't have a sealed,
full-length liner tube. I'm certainly open to thoughts on why, in 23
years of servicing SEMs, I haven't seen this problem.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:01:04 2000



From: Harrison, Gail :      Gail.Harrison-at-reichhold.com
Date: Tue, 4 Apr 2000 11:48:35 -0400
Subject: Hot Stage Retrofit

Contents Retrieved from Microscopy Listserver Archives
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Good Morning All

A colleague has recently requested analysis with a hot stage light
microscope. As I do not have one, I am looking to retrofit a very old
Reichert compound microscope with a hot stage. Is there anyone out there
who might be able to help me?

Thank you

Gail Harrison
Reichhold
919.990.8285



From daemon Tue Apr 04 15:01:07 2000



From: CrushStone-at-aol.com
Date: Tue, 4 Apr 2000 12:25:46 EDT
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At Martin Marietta Labs (1977), Harry O. and myself were responsible for
training, use, and maintenance of an ISI Mini-SEM. One morning we found the
SEM with a blown filament after late-night operation by user or users
unknown. Further investigation revealed the remains of a house fly affixed
with Aquadag to a specimen stub -- and dispersed throughout the microscope.
The innards of the house fly in the microscope prevented the SEM from
reaching operating vacuum. It took two days to adequately clean the column
and the vacuum system.

Apparently the guilty party wanted to examine a housefly in the SEM, but did
not think about it exploding in high vacuum. The fact the fly was not
sputter coated suggested the likely guilty party. He later confessed and was
denied further access without close supervision.

Steve Stokowski
Stone Products Consultants
10 Clark St., Ste. A
Ashland, Mass. 01721
508-881-6364 (ph. and fax)
http://members.aol.com/crushstone/petro.htm



From daemon Tue Apr 04 15:01:15 2000



From: rlvaughn-at-unmc.edu
Date: Tue, 4 Apr 2000 12:44:43 -0500
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
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Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Tue Apr 04 15:01:15 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 04 Apr 2000 13:30:42 -0400
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Friends and colleagues

I realize this thread is getting tired, but as this one happened to me just
yesterday, perhaps you'll bear with me as I tell it.

For those of you that don't know the instrument, the XL30 ESEM, like most
modern SEM's, displays its image as 256 intensity levels on a computer
monitor. Presumably the colour could be set to whatever the user chooses,
but we use the default black-and-white.

I had a young photorapher working for a prestigious magazine who needed an
SEM photograph of a human hair. I had the microscope set up before he
arrived, so there was an image on the screen as he walked in. After a few
minutes, he asked "I thought you said this was one of your own hairs".
"Yes", I replied. He looked puzzled, looking closely at my head, then said
"Did you take one of the grey ones?"

Tony.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Tue Apr 04 15:01:20 2000



From: rlvaughn-at-unmc.edu
Date: Tue, 4 Apr 2000 12:31:43 -0500
Subject: re no more stories

Contents Retrieved from Microscopy Listserver Archives
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clogged? I have a whole 13 since yesterday among another dozen of real
junk.

I can't believe some of these things have happened! I have been trying to
dismiss all the anti user ideas that the faculty had here. Don't let
students use it, don't let researchers use it, don't teach them how -- they
can't remember how to use it. I was once a student and learned how to tear
down an old ISI SEM to clean after a filament change (with manual valving).
So if taught and monitored I said, then they should enjoy the fun part of
EM. It's worked so far. (cross my fingers and knock on wood)

I don't have any stories but heard at a meeting of a visiting researcher
from the Far East that was pipeting Osmium by mouth. When corrected he
shook his head yes but was found doing it again so they banned him from the
lab.

Rick Vaughn



From daemon Tue Apr 04 15:01:24 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 04 Apr 2000 15:29:11 -0700
Subject: Re: Hot Stage Retrofit

Contents Retrieved from Microscopy Listserver Archives
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Gail,

Most stages have the holes necessary to accept any of the conventional hot
stages.

By the way, do you know which model of Reichert do you have?

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 11:48 AM 4/4/00 -0400, Harrison, Gail wrote:
} ------------------------------------------------------------------------
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From daemon Tue Apr 04 15:01:25 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Tue, 4 Apr 2000 13:35:27 -0600
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
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Just for clarification:

our software, the EFI module for analySIS, DOES take into account the
lateral shift typical for the dissecting microscopes. It corrects for
this shift first before attempting to reconstruct the final image.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Rosemary White [mailto:Rosemary.White-at-pi.csiro.au]
Sent: Tuesday, April 04, 2000 3:45 AM
To: microscopy-at-sparc5.microscopy.com


Dear Dave,

There are a couple of options. Autoquant will do this, as will modules
from Soft Imaging Systems. I think some Zeiss software can do this too,
as
long as there is no lateral shift in sequential images (as is found in
some
dissecting scopes). These are packages we've tried, there may well be
more.

cheers,
Rsoemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 04 15:53:04 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Tue, 04 Apr 2000 15:31:12 -0600
Subject: Service Horrors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My horror story is about 3 very competent service engineers, all from
the same company, who each blew the window in the same EDX detector in
their own turn. Engineer #1 was just unlucky, I think. He was
installing a STEM unit and the window just popped. Engineer #2 had
disassembled the TEM goniometer and for some unknown reason turned on
the roughing pumps. Evidently the inrush of air increased pressure on
the window enough to cause it to pop. Engineer #3 just didn't listen
to me. He had insisted that my detector bellows was causing a very
small leak. He had taken the detector off 3 times, sure he would
finally demonstrate that without the detector the TEM would hold
vacuum. Each time, though, the vacuum would drift and he'd go find
and solve another leak. After the 3rd time, I told him to not mess
with the detector anymore for fear he would break it. Two days later
after I returned from giving an out-of-town lecture, the detector was
off the scope and the window was indeed blown...and the vacuum leak
was still not solved. I ended up paying for a percentage of the last
repair because the engineer had gotten permission to remove the
detector from my trainee technician (2 months experience). The
bellows was proved to be tight and a new butterfly valve solved the
vacuum leak.

The companies and engineers remain nameless.

Chuck Butterick
Engineered Carbons, Inc.
Borger, TX



From daemon Wed Apr 05 07:54:43 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Tue, 4 Apr 2000 15:46:46 -0700
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh no..... I really didn't mean to start another bio vs materials war. I
just meant to point out that we have a variety of interests represented here
and not every item is going to be relevant to every reader at every time (as
a pharmacist, I have done some pharmacognosy in the past and enjoyed it). If
you don't like something, just use the delete button and let others make
their own judgements.

Pax everyone!

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Tuesday, April 04, 2000 10:45 AM
To: Microscopy-at-sparc5.microscopy.com


Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Wed Apr 05 07:54:43 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Tue, 4 Apr 2000 15:48:08 -0700
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh no..... I really didn't mean to start another bio vs materials war. I
just meant to point out that we have a variety of interests represented here
and not every item is going to be relevant to every reader at every time (as
a pharmacist, I have done some pharmacognosy in the past and enjoyed it). If
you don't like something, just use the delete button and let others make
their own judgements.

Pax everyone!


Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Tuesday, April 04, 2000 10:45 AM
To: Microscopy-at-sparc5.microscopy.com


Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Wed Apr 05 07:54:44 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Tue, 4 Apr 2000 16:31:14 -0700
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh no..... I really didn't mean to start another bio vs materials war. I
just meant to point out that we have a variety of interests represented here
and not every item is going to be relevant to every reader at every time (as
a pharmacist, I have done some pharmacognosy in the past and enjoyed it). If
you don't like something, just use the delete button and let others make
their own judgements :-)

Pax everyone!

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Tuesday, April 04, 2000 10:45 AM
To: Microscopy-at-sparc5.microscopy.com


Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Wed Apr 05 07:54:45 2000



From: EBMet-at-aol.com
Date: Tue, 4 Apr 2000 19:48:49 EDT
Subject: Confirmation of New Subscription

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is to confirm my subscription to the listserver and to ask my first
question.

I wish to purchase a PC based scanner with a transparency adaptor to scan in
TEM negative plates for digital processing and output. I'd like to keep the
cost of the hardware under $1000. What hardware is available out there? I
have looked at specs for HP 6390C and UMAX Powerlook III. What minimum specs
should I be looking for? Thanks.

Elliot Brown



From daemon Wed Apr 05 07:54:47 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Tue, 4 Apr 2000 13:59:22 -0700
Subject: RE: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Praveena:

You don't say what the diameter of the wires is. If possible, you might try
reducing the size of the sample area that needs to be sectioned. I don't
know if you are sectioning at room temperature or cryo. The Tg of PP is
around -19C, so if you are not doing cryo you might want to give that a try.
A diamond knife should work better. Also, depending on what it is you are
trying to see, if you can live with slightly thicker sections then I would
try that.

Hope this helps!

Jordi Marti
Honeywell.



From daemon Wed Apr 05 07:55:00 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 5 Apr 2000 12:08:39 +1000
Subject: CPD windows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have received a another message concerning CPD window material (in the
earlier string a Polaron/ VG manager noted that their windows were not quartz,
but some unspecified superior material.

Ted Pella has advised that they are now using -

"sapphire windows (2 of them, one under the other), which are in turn covered
by the bullet proof shield. I think sapphire has about 50% greater strength
compared to quartz (about 9,000 psi vs about 6,000) - that's why we use
sapphire, for greater safety. We have never
heard of any accident with our unit.

We added the bullet proof shield ("Lexan") to add a second safety
measure for the viewing ports.

Three other safety measures are included: rupture disc,
over-temperature switch and over-pressure switch."

I have no doubt that all manufacturers of CPD are most concerned and make these
units safe; afterall one accident could cost a year's manufacturing cost.
Its nice to know what technology is now in use, thank you Ted Pella.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com



From daemon Wed Apr 05 07:55:00 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Wed, 05 Apr 2000 00:36:53 -0400
Subject: Re: SEM: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


shAF,

Regarding the anomalous variation in beam current with emission which you
observe between your instruments:

This is a good demonstration of the fact that as far as emission is
concerned, more isn't always better. I commonly compare the problem of
getting the best performance from the gun to the idea of trying to squirt
water through a knothole some distance away -- the critical parameter isn't
the flow rate of the hose, but rather the ability to direct it into a
focused stream. In reality, the vast majority of the emission never makes
it into the column (compare the emission current to your maximum beam
current) so the quality of the emission is more important than the
quantity. The dynamic of the triode gun is that as you reduce the bias
(thereby increasing the size of the emitting area of the filament and
generating more emission) you also reduce the amount of "focusing" and thus
the emission is less convergent and a smaller fraction passes through the
anode. Whether there is a beam increase or decrease depends on the specifics
of where the gun is operating. In theory, you should be able to observe
this "reverse trend" ( beam current goes down as emission increases) by
reducing the bias sufficiently far on any instrument -- though a particular
instrument may not have the range of bias adjustment to permit this.

Hope this helps.

Fred Schamber
RJ Lee Instruments Limited

=shAf= wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I've just noticed behavior which is different for my 2 e-beam
} instruments. If I bias my microprobe's gun for less emission, the
} beam current measured in a specimen faraday cup also goes down.
} However, if I bias my SEM's gun for less emission, the beam current
} (measured similarly) goes up(???). This may mean simply a shallower
} optic angle for the SEM and the anode allowing more electrons to pass,
} but I thought I'd throw the observation out there.
}
} cheerios, shAf
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - ICQ 210524
} Geological Science's Electron Probe Facility - University of Oregon
} mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/



From daemon Wed Apr 05 07:55:04 2000



From: Colin Reid :      creid-at-tcd.ie
Date: Wed, 5 Apr 2000 06:46:50 +0100
Subject: RE: Microscope Horror( Humour ) Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reminds me of a recent thing that happened here:-

A former colleague spent approx. six hours ( over three separate days )
analysing metal wear particles on the SEM. He was doing the work with a
French engineer who had prepared the samples. She told which particles to
analyse and he dutifully analysed them. I happened to pass and glanced at
the screen and asked why they were analysing paper fibres. They both
insisted that I was wrong ( unfortunately they didn't take my bet ! ), until
I suggested that they try using the BSE detector. Strangely they had lower
contrast than the Al stub. The engineer is almost finished her PhD now and
my colleague has moved on to become a forensic scientist. Just goes to
show the customer is always right ?


Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2,
Rep. of Ireland.
Tel: 353-1-6081820
Fax: 353-1-6770438
Email: creid-at-tcd.ie
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm


-



From daemon Wed Apr 05 07:55:20 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Wed, 05 Apr 2000 12:28:40 +0200
Subject: Re: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If You are interested please have a look at our High performance slow-scan CCD
for TEM at
http://www.proscan.de/pakete1.htm

Mark YEADON schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Mike,
}
} You could check out Soft Imaging Systems' MegaView II at:
}
} http://www.soft-imaging-web.de/ {http://www.soft-imaging-web.de/}
}
} I'd like to hear other recommendations too...
}
} Mark
}
} %%%%%%%%%%%%%%%%%%
} Mark Yeadon
} Senior Research Fellow
} Institute of Materials Research and Engineering
} 3 Research Link
} Singapore 117602
}
} Assistant Professor
} Department of Materials Science
} National University of Singapore
} Singapore 119260
}
} TEL: (+65) 874 8591
} FAX: (+65) 872 0785
} Email: m-yeadon-at-imre.org.sg {mailto:m-yeadon-at-imre.org.sg}
}
} -----Original Message-----
} From: Michael Coviello [SMTP:coviello-at-mae.uta.edu]
} Sent: Thursday, March 23, 2000 4:44 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM-Digital camera recommendations
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
}
} Hi Ya'll:
} We are looking for a CCD camera for our Philips 430 TEM. We would
} like
} to get the best camera for the best price, e.g., either buying a
} used
} camera or a new non-Gatan camera (Gatan seems to be twice as
} expensive
} as the others). We would be using the camera for bright field and
} high
} resolution TEM of materials (semiconductors) rather than for
} biological
} specimens. Does anyone have a camera they would like to sell/donate
} or
} does anyone have recommendations as to a less-expensive camera that
} they
} know can be used for materials applications.
} Thanks, Mike Coviello
} Lab Manager
} University of Texas -at- Arlington
}

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Wed Apr 05 08:13:44 2000



From: Robert.Carlton-at-aventis.com
Date: Wed, 5 Apr 2000 07:57:05 -0500
Subject: Polishing SRM 482 Gold-Copper Wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

I am having some difficulties obtaining a good, clean polish on gold-copper
wires (SRM 482). I have embedded the wires in a thermoset resin, backed the
wires with epoxy and polished with successively smaller SiC grit. I have
tried a variety of final polishes including .25 um diamond, vibratory
polishers, etc. I can achieve an excellent metallographic finish with regard
to smooth surfaces, but I cannot remove some small spots from all of the
wires. These are iron red in polarized light and do not have the morphology
of the polishing compounds. A qualitative analysis by EDS does not show any
elements other than copper and gold. Does anyone have experience polishing
these alloys? Is there something I'm missing?

Thanks to all
Robert Carlton
Aventis Pharmaceuticals
robert.carlton-at-rp-rorer.com




From daemon Wed Apr 05 08:25:18 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Tuesday, April 4, 2000
Subject: Fwd: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


What a great basis for an SEM exam question. I'm going to tuck this one away until needed!
Debby


Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


shAF,

Regarding the anomalous variation in beam current with emission which you
observe between your instruments:

This is a good demonstration of the fact that as far as emission is
concerned, more isn't always better. I commonly compare the problem of
getting the best performance from the gun to the idea of trying to squirt
water through a knothole some distance away -- the critical parameter isn't
the flow rate of the hose, but rather the ability to direct it into a
focused stream. In reality, the vast majority of the emission never makes
it into the column (compare the emission current to your maximum beam
current) so the quality of the emission is more important than the
quantity. The dynamic of the triode gun is that as you reduce the bias
(thereby increasing the size of the emitting area of the filament and
generating more emission) you also reduce the amount of "focusing" and thus
the emission is less convergent and a smaller fraction passes through the
anode. Whether there is a beam increase or decrease depends on the specifics
of where the gun is operating. In theory, you should be able to observe
this "reverse trend" ( beam current goes down as emission increases) by
reducing the bias sufficiently far on any instrument -- though a particular
instrument may not have the range of bias adjustment to permit this.

Hope this helps.

Fred Schamber
RJ Lee Instruments Limited

=shAf= wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I've just noticed behavior which is different for my 2 e-beam
} instruments. If I bias my microprobe's gun for less emission, the
} beam current measured in a specimen faraday cup also goes down.
} However, if I bias my SEM's gun for less emission, the beam current
} (measured similarly) goes up(???). This may mean simply a shallower
} optic angle for the SEM and the anode allowing more electrons to pass,
} but I thought I'd throw the observation out there.
}
} cheerios, shAf
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - ICQ 210524
} Geological Science's Electron Probe Facility - University of Oregon
} mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/






From daemon Wed Apr 05 17:23:29 2000



From: Ed Vicenzi :      vicenzi-at-volcano.si.edu
Date: Wed, 05 Apr 2000 08:51:41 -0400
Subject: SEM Lab Manager Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position available at the Smithsonian Institution:

The National Museum of Natural History in Washington, DC is seeking an
experienced electron microscopist to fill a vacancy for SEM laboratory
operation and management. The SEM facility is designed to serve both the
biological and geological research communities in the museum, and houses
two recent model SEMs and one state-of-the-art environmental microscope (to
be installed in mid-2000). The principal responsibilities include training
staff members and visiting scientists in proper use of equipment and theory
of electron generation and detection, maintenance and troubleshooting all
instrumentation (in conjunction with full service contracts), evaluation of
new developments in SEM technology, and supervision of a support staff
member. The successful applicant will also have the opportunity to gain
experience in Focused Ion Beam (FIB) Microscopy and high spatial resolution
secondary ion mass spectrometry HR SIMS.

This position will fill a federal government vacancy and is offered at the
GS11 ($42,724) or GS12 ($51,204) levels, and carries promotion potential to
grade GS 13. U.S. citizenship is required for this federal position. To
obtain information concerning this vacancy call our automated Jobline at
(202) 287-3102 (24 hours, 7 days a week), press 9 and request vacancy
announcement 00MQ-2069. Announcements will be available beginning April
18th and applications must be received by May 16th, 2000. If questions
arise after receiving and reading through the vacancy announcement please
contact: Dr. Edward Vicenzi (Chair, SEM Lab Manager Search Committee) at
vicenzi-at-volcano.si.edu. The Smithsonian Institution is an equal
opportunity employer.


PLEASE NOTE: this position is open from April 18th to May 16th, 2000.






~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Edward P. Vicenzi
Smithsonian Institution
Department of Mineral Sciences
Washington, DC 20560-0119

(202) 357-2594
(202) 357-2476 (fax)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Wed Apr 05 17:23:31 2000



From: Thomas A. Baginski :      tombg-at-bictom.usuf1.usuhs.mil
Date: Wed, 5 Apr 2000 10:08:26 -0400
Subject: Re: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A couple decades + ago while provding TEM services for the NIH neurology
group, the Neurosurgeons were impatiently awaiting the Biopsy results of
their surgical patient. The frozen section was not conclusive, and so they
needed a TEM result which would confirm their findings. Not being satisfied
with my answer that the results will take a couple days, or one day at the
very earliest, the surgeon sent his first resident up to my lab and he began
rummaging through my supply cabinets so he can prepare the sample quickly
himself. This is before microwave embedding and fixation. He told me that
he was instructed (by my boss) to cut a very thin slice of tissue (use a very
sharp razor), coat it with a lot of glutaraldehyde (straight out of the
bottle) and stick it into the scope (a Joel 100 at that time). He would be
waiting in the OR with the patient until he got the result. He declared
that he did not need any training. ... I was speechless and could not
believe this attempt...I watched the fate with amusement.
I Volutarily moved on to safer grounds thereafter.

Thomas Baginski

Thomas A Baginski, Room G-230
Technical Coordinator for Microscopy
Uniformed Services University of the Health Sciences
4301 Jones Bridge Road
Bethesda, MD 20814-4799

Voice Phone: 301 295 5691
Fax: 301 319 8218
Email: tbaginski-at-usuhs.mil
Alt Email: tombg-at-bictom.usuf1.usuhs.mil
WebSite: { {http://bic.usuf1.usuhs.mil/index.html



From daemon Wed Apr 05 17:23:36 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 05 Apr 2000 15:35:17 +0100
Subject: folds in semi-thin resin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All

What is the current folklore on avoiding folds/creases in semi-thin
resin sections? I am cutting a worm, approx. 1 mm in diamter, for
light microscopy and photography. It is typical Annelid, i.e.
external cuticle, muscular body wall with inner coelomic space
containing another hollow tubular structure (the gut). It is embedded
in araldite for normal TEM. I have tried drying sections onto glass
slides at 35, 60, 70 and 110 degrees. They all wrinkle prior to
Toluidine Blue staining on hotplate. I have also stained sections by
flotation overnight prior to drying on slides. Help!

Keith Ryan
Marine Biological Association
Plymouth UK



From daemon Wed Apr 05 17:23:37 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Wed, 05 Apr 2000 09:49:47 -0400
Subject: Re: Lessons in horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We've been fortunate here that we've not had too many horror stories (at
least funny enough to print here) even though we've had many users of
greatly differing abilities. Of the mild catastrophes we've had, all have
been invaluable for teaching purposes. They teach new users what can go
wrong and how you can become inadvertently famous by not thinking. They've
also taught me how to write instructions to prevent accidents, and how
creative people can be in coming up with new ways to run the microscope. I
routinely tell stories of past users (without revealing identities) to
novices to lighten the long hours of instruction. I've also gotten into the
habit of attaching the price tag (figuratively) to fixing different
components of the scope. There are the famous $18 plastic knobs, } $300
hexrings, and the $50k if you hit your head on the specimen holder while
it's in the scope (actually happened!). This "scared straight" tactic is
always tempered with humor and encouragement so not to paralyze the faint
of heart. And most of all, we don't heap blame on users for errors. We
encourage honesty, lest we get mysterious cases that take much longer to
decipher (such as the case of the missing holder tip).
Ciao for now,
Ken




From daemon Wed Apr 05 17:23:39 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 5 Apr 2000 08:57:58 -0700
Subject: RE: SEM: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred writes ...

}
} Regarding the anomalous variation in beam current with
} emission which you observe between your instruments:
}
} ...
} ... The dynamic of the triode gun is that as you reduce
} the bias (thereby increasing the size of the emitting area of the
} filament and generating more emission) you also reduce the
} amount of "focusing" and thus the emission is less convergent
} and a smaller fraction passes through the anode.
} Whether there is a beam increase or decrease depends
} on the specifics of where the gun is operating.
} In theory, you should be able to observe
} this "reverse trend" ( beam current goes down as emission
} increases) by reducing the bias sufficiently far on any
} instrument -- though a particular instrument may not have
} the range of bias adjustment to permit this.
} ...

Are you implying any "optimization" at the 1st cross-over by
exploring the relationship of emission versus the number of electrons
which get past the anode?? (... as if, the point at which decreasing
the emission also decreases the beam current implies less than
optimized (?) ...). What are your thoughts for this relationship
regarding the "brightest crossover" versus "extended life of the
electron emitter"??

=shAf= :o)



From daemon Wed Apr 05 17:23:43 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Wed, 5 Apr 2000 12:52:35 -0500
Subject: EM/LM: Antibodies to fluorescein

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Hello Microscopists:

I'm interested in localizing fluorescein-tagged gene probes for microscopy
(in situ hybridization, light and EM) and blotting applications. Does
anyone have any recommendations for the best (strongest labeling)
anti-fluorescein antibody, or any experience comparing different clones,
polyclonal vs. monoclonal, or different suppliers?

Thanks in advance,

Rick Powell
Nanoprobes, Incorporated




From daemon Wed Apr 05 17:23:46 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Wed, 5 Apr 2000 14:00:15 -0400
Subject: Gun Bias

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Hi

Yes it is very interesting isn't it?

For those TEM operators who have not seen the effect of changing bias on
the electron beam there is a neat experiment.

1. At "gun saturation" bring the condenser to crossover and magnify
this to fill the screen.

2. Change the bias or emission control (not the filament heating)

Either a) The gun de saturates, if so turn the bias control in the
other direction to increase the bias field
Or b) The intensity increases slightly (the subject of current
mail)
Or c) The intensity decreases

If you see (b) the filament is in an position within the cathode that
allows the full optimisation of the emission system; in my experience few
people run under these conditions.

If you see (c) the filament is not in the optimised position within the
cathode.

For SEM operators

1. Set up in the wave form or graph mode

2. Watch the trace as you increase the bias field (emission reduces)

Either a) With the filament position optimised for efficiency the
trace should rise slightly
Or b) With a filament positioned away from this point the trace
will fall.

For those who do not understand the bias or emission control relationships-

A BIAS control will reduce the emission current when turned clockwise,
higher bias.

An EMISSION control will reduce the emission current when turned
anticlockwise, higher bias, or of you like turn up an emission control and
the reduction in the bias field allows an increase in emission current.

They are acting on exactly the same area of the high voltage circuit but
are simply wired in a different way.

Have fun

Steve Chapman
Senior Consultant
Protrain - for professional training in EM world wide
e-mail protrain-at-emcourses.com
web site www.emcourses.com
Tel 44+ 1280 814774 Fax 44+ 1280814007



From daemon Wed Apr 05 17:23:46 2000



From: A. Greene :      ablue-at-io.com
Date: Tuesday, April 04, 2000 2:30 PM
Subject: Re: re no more horror

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Hello folks,
I was traveling and really wanted to zip in three short "horror stories"
before the thread got yanked or burned. [1] Eons ago (Actually maybe 25
years) I was a service engineer with Philips and went to perform routine
maintenance on a very early EM-300. I always cleaned the windows and in
this instance, I found the smaller, left, projection window to be Plexiglas!
No one would tell me how long the ersatz window had been in place but I
really made my point when I demonstrated, with a Geiger Counter, that lots
of x-rays were getting sprayed into the room. They quickly got as new
window. I also had a similar experience when a customer stuck the Aluminum
shipping plate in place of a broken window. They also bought a nice new
leaded glass window. See, the window stories are true.
[2] Some of the older microscopes used Mercury Diffusion Pumps to increase
pumping speed. They were usually operated in tandem with the Oil Diffusion
Pump. In one of the not terribly uncommon disasters where a blast of
Mercury blasts up the column and turns all the beautifully Gold plated brass
pieces into a amalgam covered mess, a service engineer who was particularly
resourceful found out that Iodine readily combines with Mercury. He
sprinkled Iodine crystals all over the contaminated parts of the column.
Not long later, there was an explosion! No one was physically hurt, and I
heard the microscope company cleaned up the mess. [3] Our laboratory was
below the level of a creek so when there were sump pump failures, water
would seep onto the floor, fairly quickly. A lovely and dedicated graduate
student was working late a night when one of these pump failures occurred
and she ended up with her feet under water while running our old JEOL 35C
Scanner. I found her and said she would have to stop, as I yanked the wall
switch. She was angry with me for interrupting her research until I was
finally able to convince her she was very close to electrocuting herself.

Best regards,

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200 Phone: 512/282-5507 FAX 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIRS
-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
{"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed Apr 05 17:23:56 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 5 Apr 2000 16:20:57 -0400
Subject: RE: Silicone oils & greases

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The problem with using silicone compounds in electron microscopes is that
if they get on parts where they are struck by the electron beam they break
down to produce siliceous compounds which are non-conductive and so collect
a static electric charge and deflect the electron beam in undesirable ways.
These decomposition compounds are also very insoluble, and become difficult
to remove. I recall our RCA EML microscope, which came charged with
silicone DP oil, developed such deposits on the objective and condenser
apertures. Fortunately, these apertures were made of platinum, and so we
could clean them by heating them in a Bunsen burner to convert the deposits
to silicon oxides and then treating them with concentrated hydrofluoric
acid to dissolve these oxides - something that would be frowned on in most
laboratories these days.

Because of the potential for generating this kind of a problem, I can see
no reason whatsoever for using either a silicone grease or a silicone oil
in a modern electron microscope. In fact, when I was managing electron
microscope laboratories I refused to allow any of these materials in
laboratories out of fear that some inexperienced person would use them on
one of the instruments.

The reason for using a vacuum grease on an O-ring (or gasket) is to provide
enough lubrication so that the O-ring will slide enough to fill the O-ring
groove uniformly, without forming bumps or creases that can cause a leak.
The grease should not be required to produce the basic vacuum seal - the
groove should be smooth enough so that it could seal properly without the
grease if the O-ring fitted into position properly. Thus, only enough
grease should be applied to give a barely visible sheen to the O-ring -
gobs and globs are not needed. While the silicone high vacuum grease is
indeed a good lubricant for O-rings, it is no better than the Brayco and
Krytox greases, which are based on polyphenylether compounds, and which do
not introduce the possibility of having insoluble siliceous compounds
formed on critical parts of the electron optical column. The function,
use, and characteristics of vacuum greases are discussed in more detail in
Chapter 10 of the book "Vacuum Methods in Electron Microscopy"

I also would not use a silicone fluid in a diffusion pump on an electron
microscope, nor other equipment used in preparing electron microscopy
specimens, for the same reason described above. Several diffusion pump
fluids are now available that are nearly as stable to thermal and oxidative
degradation as the silicone fluids, and which have vapor pressure
characteristics that are comparably favorable. These include the
Santovac-5 and Excello-54 polyphenyl ether fluids and the Lion-S and
Alcatel-220 eicosyl naphthalene fluids, all of which will produce levels of
vacuum as good as the DC 705 silicone fluid. These fluids are discussed in
more detail in Section 5.4 of Vac. Meth. in EM.

The problem of removing silicone compounds from parts that have become
contaminated with them is a very difficult one, Because these compounds are
usually very viscous, and are not readily soluble. The Dow Corning
Company, which manufactures them, recommends repeated wiping with cloth
pads moistened with toluene, xylene, trichloroethylene, or
perchloroethylene.

I have recently had fair success cleaning diffusion pump fluids off metal
parts by first wiping the parts with dry paper towels to remove the bulk of
the fluids, then spraying the surfaces with Tilex Soap Scum Remover and
scrubbing them with a cloth pad, then rinsing them with hot running water.
By repeating this process several times I have been able to get acceptable
results in several instances. (I remove the water by rinsing with
isopropyl alcohol, and then dry with a gas blaster.) It might be difficult
to adapt this process to cleaning internal parts of an electron microscope,
however. Other cleaning procedures are described on pp. 69 - 74 of Vac.
Meth. in EM.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Wed Apr 05 17:23:56 2000



From: mcalabrese-at-rsc.rockwell.com
Date: Wed, 5 Apr 2000 14:23:37 -0700
Subject: Re: Polishing SRM 482 Gold-Copper Wires

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Robert- It could be that the small spots are copper oxide as this polarizes to a
reddish color. Could you check for oxygen with low kv EDS?

-Mike-




From daemon Wed Apr 05 17:23:57 2000



From: Michael Nesson :      nessonm-at-ucs.orst.edu
Date: Wed, 05 Apr 2000 14:40:40 -0700
Subject: Re: folds in semi-thin resin sections

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Hi Keith:

One possible work-around is to cut your sections thinner than usual for
light microscopy, down to 0.4 micro-meters, which then are more likely to
dry wrinkle- free onto your slides. These thin sections then require an
extreme stain to provide sufficient contrast. I refer you to two papers by
del Cerro et al. on such a method, utilizing Stevenel's Blue as the stain.
The articles are in Microscopa Acta Vol. 83, pp.117-121 and 217-220 (1980).

Hope this helps,
Mike Nesson

Keith Ryan wrote:

}
} What is the current folklore on avoiding folds/creases in semi-thin
} resin sections? I am cutting a worm, approx. 1 mm in diamter, for
} light microscopy and photography. It is typical Annelid, i.e.
} external cuticle, muscular body wall with inner coelomic space
} containing another hollow tubular structure (the gut). It is embedded
} in araldite for normal TEM. I have tried drying sections onto glass
} slides at 35, 60, 70 and 110 degrees. They all wrinkle prior to
} Toluidine Blue staining on hotplate. I have also stained sections by
} flotation overnight prior to drying on slides. Help!
}
} Keith Ryan
} Marine Biological Association
} Plymouth UK

--
_______________________________________________________________________
Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
(541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu




From daemon Wed Apr 05 17:23:58 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 5 Apr 2000 17:43:40 -0400
Subject: RE: Horror Stories

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I forgot about this recent one. We had a technician here who I taught how
to develop film for our JEOL 2000FX. It had been awhile since he replaced
the film, so after he left, I wanted to make sure that the film was loaded
into the cassettes with the emulsion side up. When I opened the film
cassette box, I couldn't take out the cassettes. He had put them in 180
degrees around and the slot in the film holders didn't match up with the
alignment bar going up along the side of the box. The sides of the film box
were actually bulging. I had to pry each cassette out of the box with a
screwdriver. I left most of them for him to do the next day. Before
showing him this, I innocently asked him if he had trouble putting the
holders into the box. He said that the last couple were a little hard to
put in. The good thing: he had loaded the cassettes with the emulsion side
up.
-Scott



From daemon Wed Apr 05 17:23:59 2000



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 05 Apr 2000 18:14:36 -0400
Subject: Spare LN2 carboy needed

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Does anyone have a liquid nitrogen dewar in the 20 to 40 liter range
with low evaporative losses that they would like to sell? Please
contact me off-list.

John Twilley
jtwilley-at-sprynet.com



From daemon Wed Apr 05 17:23:59 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 5 Apr 2000 12:15:12 -1000 (HST)
Subject: RE: Horror Stories

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Here's another oops.

A friend had just finished cleaning his TEM column parts with acetone, as
he had been instructed. Being an impatient young man, he quickly
reassembled everything and, as he was lowering the column back into place,
noticed a few drops of acetone had fallen into the viewing chamber and
onto the phosphorous screen. I guess he had the chamber open for cleaning
as well, because he said he quickly grabbed the canned air and aimed it
into the chamber to blow off the drops. I walked in just then to see a
cloud of yellow dust settling all over the walls and floor of the EM
lab... He cleaned out the viewing chamber as best he could, I suppose,
but there was dust in the column and pumping system for months to
come. Then there were the phosphorescent footprints and fingerprints that
appeared all over the lab for weeks! Now every time I open my viewing
chamber I hold my breath.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Apr 05 21:34:39 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 05 Apr 00 17:39:02 -0600
Subject: More TEM Scope Hazards:

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Many years ago, our lab had one "good" electron microscope-a JEOL
200.
Every few years the massive high voltage cable would short out
internally and
require replacement. I watched the service technician unscrew the lock
ring
from the high voltage tank, which was about as large as a refrigerator
to
power many vacuum tubes. He used a chair to get on top of the unit and
pull
the cable out and hand the end to me. I heard a loud snapping sound and
saw
a frightened look on the fellow's face. In a hurry to get the job done,
he
had forgotten to use an insulated grounding rod kept in the area to
bleed
off the charge in the high voltage capacitors. Although he had a small
burn
on his hand, he and I finished the cable swap with a new one.
A few years later, the instrument had a water hose leak which
flooded
all the control cable plugs at the rear of the console. The technician
had
to "rebuild" many of the plugs over a couple of days time. It ran well
for
a year or two and was shut down to make way for a newer instrument. It's
always good to discuss major service procedures with someone to ensure
that
oversights don't occur unnecessarily.
That person later rose to the higher eschelon's of his company.

Bernie Kestel
Material's Science Division
Argonne National Laboratory
9700 So. Cass Avenue
Argonne, IL., 60439



From daemon Wed Apr 05 21:34:40 2000



From: jekman-at-uwm.edu
Date: Wed, 5 Apr 2000 17:47:17 -0500
Subject: RE: Horror Stories

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To: "'Microscopy'" {microscopy-at-sparc5.microscopy.com}


Back during the asbestos craze I was the last "little indian" doing
TEM analysis, the other two left for bigger and better paying jobs
elswhere. My business manangers (who knew the EM was big and
needed electricity, but that was the extent of their knowledge)
suggested cross-training some in-house employees to assist me
with my work. The first guy walked into the scope room chewing
gum (not like a normal human being, more like a horse). I started
showing him the scope (JEOL 100sx) while he sat in the chair
infront of the scope. The phone rang so I turned to answer it.
Meanwhile that cute little handle on the camera door caught the
new guy's attention. "what's this?" I heard, and then that all too
familiar hiss followed by valves closing and pumps and power
switches clicking off. He just reached out and turned the handle,
venting the column. The scope handled the shock better than I did.
I sent the guy to lunch, and locked the door behind him.
Jon Ekman
Associate Research Specialist
University of Wisconsin Milwaukee
414-229-6471



From daemon Wed Apr 05 21:34:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 05 Apr 2000 18:51:15 -0700
Subject: Need some beautiful stereo pairs

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Hi,

A client of ours has a clever new way to present 3D images but needs some
beautiful stereo pairs to generate a set of tests. These images need to be
true stereo pairs, not anaglyphs. If you have anything you would like to
share, please contact me directly. Also, there is a possibility that these
materials may be used in future publications... of course, with credit, so
please let me know if you would like the images used (a) just for the tests
or (b) for both tests and publication, with your citation.

I look forward to hearing from you all!

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Wed Apr 05 21:34:49 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Thu, 6 Apr 2000 11:07:13 +1000
Subject: Re: Stretch?? Epoxy sections?

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Dear Hildegard,

To follow up a posting a few days ago, what epoxy are you using that does
not compress during sectioning? I am very curious!

THanks,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Thu Apr 06 07:35:35 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Wed, 05 Apr 2000 23:24:47 -0400
Subject: Re: SEM: gun emission and biasing

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{!doctype html public "-//w3c//dtd html 4.0 transitional//en"}
{html}

{blockquote TYPE=CITE}
{pre} =shAf= wrote:
        Are you implying any "optimization" at the 1st cross-over by
exploring the relationship of emission versus the number of electrons
which get past the anode??  (... as if, the point at which decreasing
the emission also decreases the beam current implies less than
optimized (?) ...).  What are your thoughts for this relationship
regarding the "brightest crossover" versus "extended life of the
electron emitter"?? {/pre}
{/blockquote}

{p} {br} Yes, the whole mechanism relies on optimization of the electron
trajectories so that they converge to a dense "crossover" in the region
of the anode opening.  Raw emission increases as bias is reduced but
the amount of focusing is also reduced so that there is an optimum point
where the maximum current is focused into the "virtual aperture" (entrance
pupil) of solid angle where it will end up hitting the specimen.
{p} Understanding the dynamics of the gun is really made much more complicated
by the self-biasing (auto-bias) circuit which nearly every thermionic microscope
uses (field emitters are, of course, a whole different story).  If
one had a direct-bias unit you could freely run the bias from low to high
and clearly see the effects.  At a very low bias, you get a flood
of emission (usually limited only by the rating of the HV power unit) and
at a sufficiently high bias you can cut the emission off entirely. 
However, because almost no one has a direct-bias setup, you can't adjust
the bias directly -- you adjust the bias resistor -- which kind of does
the same thing, but with one big difference -- you are now adjusting the
operating point of the emission-stabilization circuit.  A practical
consequence is that you simply can't achieve cut-off -- since the actual
bias is the voltage drop across the bias resistor due to the flow of emission
current, it is clearly impossible to cut off the emission current completely
(zero current would imply zero bias).  Thus, the bias resistor acts
like it is adjusting the bias directly (except in the opposite sense of
the control) for low resistor values -- but at the other end of the range
it behaves quite differently from a true bias control since it approaches
"cutoff" asymptotically rather than abruptly.
{p} Now look at the other part of the story -- the role of filament temperature. 
If one had a unit with an independent fixed bias, increasing the temperature
would make the emission rise without limit (no "saturation" knee). 
But the autobias circuit means that at some point, as one increases the
filament temperature, the increasing bias (due to the increased voltage
drop across the bias resistor) starts trying to "cut off" the emission
and you reach a stable operating point which microscopists like to call
"saturation" which is, in fact, nothing more than the operating point of
the autobias circuit.
{p} So we have two effects we are trying to match up:  (a) the need
to optimize the projection of the crossover into the column's entrance
pupil; and (b) an autobias circuit which will limit emission once a particular
emitter temperature is reached.  This is the purpose of adjusting
the bias resistor.  By setting it properly, you can make the optimal
convergence condition occur at a desireable "saturation" temperature. 
This condition of optimal convergence is measured by a quantity we call
"brightness", which is the product of the spatial and angular densities
of the beam at the crossover (high brightness means the crossover has a
small diameter and the beam doesn't diverge much -- just what you want
to get the maximum beam down the column).
{p} As to the life of the filament versus maximum brightness:  There
is a well-known equation due to Langmuir which gives the maximum brightness
which can be achieved for a given filament temperature (for a given beam
voltage and material work function).  All other things being equal,
the higher the temperature, the greater the attainable brightness. 
At the same time, the higher the temperature, the greater the rate of filament
evaporation and the shorter its life.  It should be possible on any
microscope to set it up to approach the Langmuir limit over a range of
operating temperatures (assuming appropriate wehnelt spacing and orifice
size).  So you have to make a choice, do you want high brightness
or long filament life?  You can't have both.   It's too
bad that our microscopes don't read filament temperature directly because
then everyone could easily see what the REAL variable regulating filament
life is.  When you adjust the bias resistor, you adjust the emission
stabilization operating point, and since microscopists are taught to "saturate"
the filament, you are thus establishing the filament's operating temperature
and thus its life.
{p} Hope this somewhat long-winded answer addresses your question!
{p} Fred
{br}  
{blockquote TYPE=CITE} Fred writes ...
{p} }
{br} } Regarding the anomalous variation in beam current with
{br} } emission which you observe between your instruments:
{br} }
{br} } ...
{br} } ...  The dynamic of the triode gun is that as you reduce
{br} } the bias (thereby increasing the size of the emitting area of the
{br} } filament and generating more emission) you also reduce the
{br} } amount of "focusing" and thus the emission is less convergent
{br} } and a smaller fraction passes through the anode.
{br} } Whether there is a beam increase or decrease depends
{br} } on the specifics of where the gun is operating.
{br} } In theory, you should be able to observe
{br} } this "reverse trend" ( beam current goes down as emission
{br} } increases) by reducing the bias sufficiently far on any
{br} } instrument -- though a particular instrument may not have
{br} } the range of bias adjustment to permit this.
{br} } ...
{p} =shAf=  :o) {/blockquote}
{/html}


{/html}



From daemon Thu Apr 06 07:35:40 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 5 Apr 2000 21:25:56 -0700 (PDT)
Subject: Re: folds in semi-thin resin sections

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Dear Keith,
My experience has been with parasitic helminths. Those of us who work to
any degree of regularity on whole organisms find the varying consistency
of tegument, musculature, and internal organs troublesome. Frequently the
tegument expands differently than the circular, longitudinal, and
tranverse musculature.

I have found the best solution to this situation is to adjust the hardness
of the embedding media to match the most dense material in a given
specimen. In addition to resin hardness, adequate infiltration is a must.

In previous list server responses to wrinkled sections, the techniques of
infiltration and resin hardness, as well as the use of various solvents
and heat to expand sections have been suggested.

Personally, I prefer using LR White methyl methacrylate, medium grade, or
a harder version of the epon replacements with araldite. The difference in
the appearance of the ultrastructure between these two resins will be
obvious, but not necessary objectionable. I find LR White seems to
'relax' more than that of the epoxy resins, is easier to stain with
multiple stains and requires less staining time with u.a. and
pb.cit. However, LR White is the bane of many microscopists and therefore
is not always the resin of choice.

This whole problem is interesting in that so many variables determine
section quality, e.g., how do you pick up the sections, how clean are your
microscope slides, how clean is your boat, how clean are the instruments
you use to transfer sections to the microscope slide, how thick are your
sections, on and on it goes!

My suggestion would be to make certain your slides, your glass knifes and
boats, and the utensils you use to remove the sections are CLEAN! Next,
try waiving a cotton swab dripped in cholorform very close to your
sections and see if your sections expand enough to appear smooth. I
prefer to do this step with the sections already on a droplet on a clean
microscope slide. I watch the sections under the stereoscope to look for
the smooth appearance. In some case, I actually use a heat wand in
addition to the cholorform. If you do not have a heat wand, get a
disposable heat pen (I believe most EM suppliers carry these). You can
either replace the AA batteries when they go dead or, hook the heat wand
to a variable DC transformer. Adjust the voltage to approximately 3 volts
or until the 'filament' is slightly dull red. I usually rub a finger on
my nose, GENTLY touch the filament and then turn on the voltage until the
filament produces a wisp of smoke (strange technique eh?!)

One last comment: I usually turn my hot plate to almost 250 - 300 degree
F. Watch the sections so no bubbles appear and try rocking the slide back
and forth. I would also suggest you very the duration of staining time on
the hot plate. Anything more than a minute and you will surely end up
with some portion of the section lifting from the slide and consequently,
get wrinkles.

Enough rambling... No horror stories with this one!

Cheers!
-Ken
-----------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97303



On Wed, 5 Apr 2000, Keith Ryan wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Hello All
}
} What is the current folklore on avoiding folds/creases in semi-thin
} resin sections? I am cutting a worm, approx. 1 mm in diamter, for
} light microscopy and photography. It is typical Annelid, i.e.
} external cuticle, muscular body wall with inner coelomic space
} containing another hollow tubular structure (the gut). It is embedded
} in araldite for normal TEM. I have tried drying sections onto glass
} slides at 35, 60, 70 and 110 degrees. They all wrinkle prior to
} Toluidine Blue staining on hotplate. I have also stained sections by
} flotation overnight prior to drying on slides. Help!
}
} Keith Ryan
} Marine Biological Association
} Plymouth UK
}
}
}



From daemon Thu Apr 06 07:35:48 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 06 Apr 2000 09:11:34 +0100
Subject: Re. folds in sections

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Hello again

Thanks for the responses - 14 to date.

Most mention using a heat pen or solvent vapour - I don't normally do
this in the belief that the hotplate does the job anyway. It is set
somewhere between 100-120 degrees.

My belief is that the problem is the specimen being a set of
concentric tubes of slightly varying consistency?

Cleanliness has also been mentioned! I am normally perfect - of
course (my wife told me so!).

The most interesting "wrinkle" (I had to get that pun in somewhere)
is to vary the time on the hotplate. I will play with this today. I
have been drying for a few minutes, with sections covered by a dish to
prevent any effect from draughts (I go back a bit - cutting sections
since 1969 - you'd think I have got it right by now!).

I was thinking also of removing the resin to maybe lessen the
wrinkles' effect (sodium methoxide - made with saturated NaOH in
methanol).

Keith

_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Thu Apr 06 07:35:53 2000



From: Christiane Macwilliam :      ph95ccm-at-brunel.ac.uk
Date: Thu, 06 Apr 2000 10:51:14 +0100
Subject: iNFORMATION ON VARIOUS TYPES OF MICROPROBE ANALYSIS

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To whom it may concern:
I am a physics undergraduate and I am currently trying to work on an
assignment in Microscopy. I am trying to find material on the theory and
application of electron beam and x-ray methods of analysis with
particular emphasis on SEM, TEM, EPMA, EDX, WDX, AES and XPS. I was
given this website site as a possible source of information. Any
assistance would be greatly appreciated.

Kindest Regards
Chris MacWilliam.
(ph95ccm-at-brunel.ac.uk)



From daemon Thu Apr 06 07:35:54 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Wed, 05 Apr 2000 23:24:47 -0400
Subject: Re: SEM: gun emission and biasing

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{!doctype html public "-//w3c//dtd html 4.0 transitional//en"}
{html}

{blockquote TYPE=CITE}
{pre} =shAf= wrote:
        Are you implying any "optimization" at the 1st cross-over by
exploring the relationship of emission versus the number of electrons
which get past the anode??  (... as if, the point at which decreasing
the emission also decreases the beam current implies less than
optimized (?) ...).  What are your thoughts for this relationship
regarding the "brightest crossover" versus "extended life of the
electron emitter"?? {/pre}
{/blockquote}

{p} {br} Yes, the whole mechanism relies on optimization of the electron
trajectories so that they converge to a dense "crossover" in the region
of the anode opening.  Raw emission increases as bias is reduced but
the amount of focusing is also reduced so that there is an optimum point
where the maximum current is focused into the "virtual aperture" (entrance
pupil) of solid angle where it will end up hitting the specimen.
{p} Understanding the dynamics of the gun is really made much more complicated
by the self-biasing (auto-bias) circuit which nearly every thermionic microscope
uses (field emitters are, of course, a whole different story).  If
one had a direct-bias unit you could freely run the bias from low to high
and clearly see the effects.  At a very low bias, you get a flood
of emission (usually limited only by the rating of the HV power unit) and
at a sufficiently high bias you can cut the emission off entirely. 
However, because almost no one has a direct-bias setup, you can't adjust
the bias directly -- you adjust the bias resistor -- which kind of does
the same thing, but with one big difference -- you are now adjusting the
operating point of the emission-stabilization circuit.  A practical
consequence is that you simply can't achieve cut-off -- since the actual
bias is the voltage drop across the bias resistor due to the flow of emission
current, it is clearly impossible to cut off the emission current completely
(zero current would imply zero bias).  Thus, the bias resistor acts
like it is adjusting the bias directly (except in the opposite sense of
the control) for low resistor values -- but at the other end of the range
it behaves quite differently from a true bias control since it approaches
"cutoff" asymptotically rather than abruptly.
{p} Now look at the other part of the story -- the role of filament temperature. 
If one had a unit with an independent fixed bias, increasing the temperature
would make the emission rise without limit (no "saturation" knee). 
But the autobias circuit means that at some point, as one increases the
filament temperature, the increasing bias (due to the increased voltage
drop across the bias resistor) starts trying to "cut off" the emission
and you reach a stable operating point which microscopists like to call
"saturation" which is, in fact, nothing more than the operating point of
the autobias circuit.
{p} So we have two effects we are trying to match up:  (a) the need
to optimize the projection of the crossover into the column's entrance
pupil; and (b) an autobias circuit which will limit emission once a particular
emitter temperature is reached.  This is the purpose of adjusting
the bias resistor.  By setting it properly, you can make the optimal
convergence condition occur at a desireable "saturation" temperature. 
This condition of optimal convergence is measured by a quantity we call
"brightness", which is the product of the spatial and angular densities
of the beam at the crossover (high brightness means the crossover has a
small diameter and the beam doesn't diverge much -- just what you want
to get the maximum beam down the column).
{p} As to the life of the filament versus maximum brightness:  There
is a well-known equation due to Langmuir which gives the maximum brightness
which can be achieved for a given filament temperature (for a given beam
voltage and material work function).  All other things being equal,
the higher the temperature, the greater the attainable brightness. 
At the same time, the higher the temperature, the greater the rate of filament
evaporation and the shorter its life.  It should be possible on any
microscope to set it up to approach the Langmuir limit over a range of
operating temperatures (assuming appropriate wehnelt spacing and orifice
size).  So you have to make a choice, do you want high brightness
or long filament life?  You can't have both.   It's too
bad that our microscopes don't read filament temperature directly because
then everyone could easily see what the REAL variable regulating filament
life is.  When you adjust the bias resistor, you adjust the emission
stabilization operating point, and since microscopists are taught to "saturate"
the filament, you are thus establishing the filament's operating temperature
and thus its life.
{p} Hope this somewhat long-winded answer addresses your question!
{p} Fred
{br}  
{blockquote TYPE=CITE} Fred writes ...
{p} }
{br} } Regarding the anomalous variation in beam current with
{br} } emission which you observe between your instruments:
{br} }
{br} } ...
{br} } ...  The dynamic of the triode gun is that as you reduce
{br} } the bias (thereby increasing the size of the emitting area of the
{br} } filament and generating more emission) you also reduce the
{br} } amount of "focusing" and thus the emission is less convergent
{br} } and a smaller fraction passes through the anode.
{br} } Whether there is a beam increase or decrease depends
{br} } on the specifics of where the gun is operating.
{br} } In theory, you should be able to observe
{br} } this "reverse trend" ( beam current goes down as emission
{br} } increases) by reducing the bias sufficiently far on any
{br} } instrument -- though a particular instrument may not have
{br} } the range of bias adjustment to permit this.
{br} } ...
{p} =shAf=  :o) {/blockquote}
{/html}



{/html}



From daemon Thu Apr 06 07:35:57 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 06 Apr 2000 05:41:28 -0700
Subject: Re: Silicone oils & greases

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Wil Bigelow wrote:
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} The problem with using silicone compounds in electron microscopes is that
} if they get on parts where they are struck by the electron beam they break
} down to produce siliceous compounds which are non-conductive and so collect
} a static electric charge and deflect the electron beam in undesirable ways.
} These decomposition compounds are also very insoluble, and become difficult
} to remove. I recall our RCA EML microscope, which came charged with
} silicone DP oil, developed such deposits on the objective and condenser
} apertures. Fortunately, these apertures were made of platinum, and so we
} could clean them by heating them in a Bunsen burner to convert the deposits
} to silicon oxides and then treating them with concentrated hydrofluoric
} acid to dissolve these oxides - something that would be frowned on in most
} laboratories these days.
}
} Because of the potential for generating this kind of a problem, I can see
} no reason whatsoever for using either a silicone grease or a silicone oil
} in a modern electron microscope. In fact, when I was managing electron
} microscope laboratories I refused to allow any of these materials in
} laboratories out of fear that some inexperienced person would use them on
} one of the instruments.
}
} The reason for using a vacuum grease on an O-ring (or gasket) is to provide
} enough lubrication so that the O-ring will slide enough to fill the O-ring
} groove uniformly, without forming bumps or creases that can cause a leak.
} The grease should not be required to produce the basic vacuum seal - the
} groove should be smooth enough so that it could seal properly without the
} grease if the O-ring fitted into position properly. Thus, only enough
} grease should be applied to give a barely visible sheen to the O-ring -
} gobs and globs are not needed. While the silicone high vacuum grease is
} indeed a good lubricant for O-rings, it is no better than the Brayco and
} Krytox greases, which are based on polyphenylether compounds, and which do
} not introduce the possibility of having insoluble siliceous compounds
} formed on critical parts of the electron optical column. The function,
} use, and characteristics of vacuum greases are discussed in more detail in
} Chapter 10 of the book "Vacuum Methods in Electron Microscopy"
}
} I also would not use a silicone fluid in a diffusion pump on an electron
} microscope, nor other equipment used in preparing electron microscopy
} specimens, for the same reason described above. Several diffusion pump
} fluids are now available that are nearly as stable to thermal and oxidative
} degradation as the silicone fluids, and which have vapor pressure
} characteristics that are comparably favorable. These include the
} Santovac-5 and Excello-54 polyphenyl ether fluids and the Lion-S and
} Alcatel-220 eicosyl naphthalene fluids, all of which will produce levels of
} vacuum as good as the DC 705 silicone fluid. These fluids are discussed in
} more detail in Section 5.4 of Vac. Meth. in EM.
}
} The problem of removing silicone compounds from parts that have become
} contaminated with them is a very difficult one, Because these compounds are
} usually very viscous, and are not readily soluble. The Dow Corning
} Company, which manufactures them, recommends repeated wiping with cloth
} pads moistened with toluene, xylene, trichloroethylene, or
} perchloroethylene.
}
} I have recently had fair success cleaning diffusion pump fluids off metal
} parts by first wiping the parts with dry paper towels to remove the bulk of
} the fluids, then spraying the surfaces with Tilex Soap Scum Remover and
} scrubbing them with a cloth pad, then rinsing them with hot running water.
} By repeating this process several times I have been able to get acceptable
} results in several instances. (I remove the water by rinsing with
} isopropyl alcohol, and then dry with a gas blaster.) It might be difficult
} to adapt this process to cleaning internal parts of an electron microscope,
} however. Other cleaning procedures are described on pp. 69 - 74 of Vac.
} Meth. in EM.
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237


Wil,
I have apparently always had a good handle on the problems of silicones
and e-beams. However, it still begs the question of why I haven't seen
these problems in 23 years of servicing ETECs. I've seen charging from
actual droplets present in a contaminated system, but never after
cleaning, and I KNOW I've never gotten a contaminated system really
stripped of silicones. Perhaps it's because one characteristic of ETECs
that customers have told me about is that they continue to image very
well when incredibly contaminated compared to other systems. Just a
thought.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Thu Apr 06 07:36:03 2000



From: Giovanni Casotti :      giovanni-at-bio.wcupa.edu
Date: Thu, 6 Apr 2000 08:32:26 -0400
Subject: EM Job Opening - Electron Microscopy Technician

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The Electron Microcopy Analysis Center (EMAC) at West Chester University
in Pennsylvania has an opening for a full-time electron microscopy
technician in a multiple user facility. The successful applicant will
have a minimum of a BachelorÕs degree and 3 years experience working with
electron microscopes. Responsibilities will include daily operation and
maintenance of the EMAC including training users on the instrumentation,
specimen preparation and darkroom techniques. The successful applicant
must demonstrate the necessary organizational, management and
communication skills to efficiently operate the EMAC. Applicants must
submit a cover letter describing their experience with different
instrumentation, a resume and contact information for three references.
Although not a requirement, a sample of electron micrographs showing the
applicant's work would be useful for the selection committee. All
materials are to be sent to the Department of Human Resources, 210 Carter
Drive, West Chester University, West Chester, PA, 19383. Applications
must be received by June 1 2000. Applicants must successfully complete
the interview process to be considered a finalist. AA/EOE. Women and
minorities are encouraged to apply.



From daemon Thu Apr 06 07:36:03 2000



From: gcasotti :      gcasotti-at-wcupa.edu
Date: Thu, 6 Apr 2000 08:38:34 -0400
Subject: EM Job Opening - Electron Microscopy Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Electron Microcopy Analysis Center (EMAC) at West Chester University
in Pennsylvania has an opening for a full-time electron microscopy
technician in a multiple user facility. The successful applicant will
have a minimum of a BachelorÕs degree and 3 years experience working with
electron microscopes. Responsibilities will include daily operation and
maintenance of the EMAC including training users on the instrumentation,
specimen preparation and darkroom techniques. The successful applicant
must demonstrate the necessary organizational, management and
communication skills to efficiently operate the EMAC. Applicants must
submit a cover letter describing their experience with different
instrumentation, a resume and contact information for three references.
Although not a requirement, a sample of electron micrographs showing the
applicant's work would be useful for the selection committee. All
materials are to be sent to the Department of Human Resources, 210 Carter
Drive, West Chester University, West Chester, PA, 19383. Applications
must be received by June 1 2000. Applicants must successfully complete
the interview process to be considered a finalist. AA/EOE. Women and
minorities are encouraged to apply.



From daemon Thu Apr 06 07:52:55 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 6 Apr 2000 08:27:00 -0500
Subject: Re:Need some beautiful stereo pairs

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Barbara,

I have a number of stereo pairs on my web site, some better than others...

http://woody.white.home.att.net

They are not intended for commercial use for profit, but may be used for
test
purposes. ...They were collected on my own time, but using the company's
equipment.

Woody White
McDermott Technology Inc.
-----------------------------------------------
Hi,

A client of ours has a clever new way to present 3D images but needs some
beautiful stereo pairs to generate a set of tests. These images need to
Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Thu Apr 06 07:52:56 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 6 Apr 2000 07:40:00 -0500
Subject: RE: Polishing SRM 482 Gold-Copper Wires

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Robert Carleton:
Have ypu considered the possibility that the red spots are particles
of Cu2O? This compound will show up as red partcles in polarized light.

Sam Purdy
National Steel Technical Center
Trenton, MI
V 734-676-2682
F 734-676-2030
spurdy-at-nationalsteel.com

} ----------
} From: "Robert.Carlton-at-aventis.com"-at-sparc5.microscopy.com
} Sent: 5, April 2000, 8:57 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Polishing SRM 482 Gold-Copper Wires
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all,
}
} I am having some difficulties obtaining a good, clean polish on
} gold-copper
} wires (SRM 482). I have embedded the wires in a thermoset resin, backed
} the
} wires with epoxy and polished with successively smaller SiC grit. I have
} tried a variety of final polishes including .25 um diamond, vibratory
} polishers, etc. I can achieve an excellent metallographic finish with
} regard
} to smooth surfaces, but I cannot remove some small spots from all of the
} wires. These are iron red in polarized light and do not have the
} morphology
} of the polishing compounds. A qualitative analysis by EDS does not show
} any
} elements other than copper and gold. Does anyone have experience
} polishing
} these alloys? Is there something I'm missing?
}
} Thanks to all
} Robert Carlton
} Aventis Pharmaceuticals
} robert.carlton-at-rp-rorer.com
}
}
}




From daemon Thu Apr 06 17:24:31 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Thu, 6 Apr 2000 09:24:31 -0400 (Eastern Daylight Time)
Subject: Re: RE: Horror Stories

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On Wed, 5 Apr 2000 17:47:17 -0500
"jekman-at-uwm.edu"-at-sparc5.microscopy.com wrote:

.....
} Meanwhile that cute little handle on the camera door caught the
} new guy's attention. "what's this?" I heard, and then that all too
} familiar hiss followed by valves closing and pumps and power
} switches clicking off. He just reached out and turned the handle,
} venting the column.

I'd be willing to bet that almost everyone with a JEOL TEM can tell a
similar story.
I do wish they would do something about that handle...it sits there
inviting itself to be turned. I've inadvertanly vented the scope
myself several times by hitting it with the back of the chair.

WL Steffens, Ph.D
Dept. of Pathology
University of Georgia



From daemon Thu Apr 06 17:24:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 6 Apr 2000 23:27:03 +1000
Subject: RE: Horror Stories

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Great thread. I think many an old timers could fill a book with those stories
by themselves.
Why though nothing autobiographical? There is no shame attached if the horror
was a learning experience. We all know that education is expensive.

At only 23 years of age and only 18 months of intermittent "user experience" I
found my self in charge of a small EM Lab with a Phillips 100C as the center
piece. Interesting TEM, with the column in the near horizontal position and a
transmission viewing screen.

There was some minor leak problem with the specimen holder. That TEM design
prepumped around the holder on partial insertion, while a spring loaded ball
sealed that space from the column. The holder was then pushed in completely and
that pushed that ball aside. Simple and effective.

To fix the ball seal I had to open the column and so I also cleaned various
bits and pieces and re-assembled. Pumped and realigned, I then pulled the
specimen holder out, whereupon the TEM inhaled very deeply. I later found that
that spring loaded ball assembly would fit 180 degrees reversed quite well, but
in that position the ball would not roll back to cover the seal when the
specimen holder was withdrawn.

The main damage was a clear center on that lovely transmission viewing screen,
the coating had been vacuumed. It cost $300 then, which is more like $2000
today and I had another opportunity to hone my maintenance skills, cleaning
column, oil and Hg pumps.

It was a very lonely job, with so little experience running that lab without
any other assistance, but I never learned so much as during those 18 months in
that lab.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, April 06, 2000 8:15 AM, Tina Carvalho [SMTP:tina-at-pbrc.hawaii.edu]
wrote:
}
} Here's another oops.
}
} A friend had just finished cleaning his TEM column parts with acetone, as
} he had been instructed. Being an impatient young man, he quickly
} reassembled everything and, as he was lowering the column back into place,
} noticed a few drops of acetone had fallen into the viewing chamber and
} onto the phosphorous screen. I guess he had the chamber open for cleaning
} as well, because he said he quickly grabbed the canned air and aimed it
} into the chamber to blow off the drops. I walked in just then to see a
} cloud of yellow dust settling all over the walls and floor of the EM
} lab... He cleaned out the viewing chamber as best he could, I suppose,
} but there was dust in the column and pumping system for months to
} come. Then there were the phosphorescent footprints and fingerprints that
} appeared all over the lab for weeks! Now every time I open my viewing
} chamber I hold my breath.
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}



From daemon Thu Apr 06 17:24:31 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 06 Apr 2000 09:30:26 -0500
Subject: Yellow dust clouds.....beware

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tina (Weatherby) Carvalho wrote:
=================================================
Here's another oops.

A friend had just finished cleaning his TEM column parts with acetone, as he
had been instructed. Being an impatient young man, he quickly reassembled
everything and, as he was lowering the column back into place, noticed a few
drops of acetone had fallen into the viewing chamber and onto the
phosphorous screen. I guess he had the chamber open for cleaning as well,
because he said he quickly grabbed the canned air and aimed it into the
chamber to blow off the drops. I walked in just then to see a cloud of
yellow dust settling all over the walls and floor of the EM lab... He
cleaned out the viewing chamber as best he could, I suppose, but there was
dust in the column and pumping system for months to come. Then there were
the phosphorescent footprints and fingerprints that appeared all over the
lab for weeks! Now every time I open my viewing chamber I hold my breath.
===============================================================
This might be a lot more serious, healthwise than just another "oops".

Until relatively recently, virtually all TEM viewing screens were coated
with a Cd-containing phosphor. In recent years there has been an attempt to
use non-Cd containing substitute materials (because of their much lower
toxicity levels) but none really seem to be quite as good as the original Cd
containing phosphors. That is why most of the bulk manufacturers of the Cd-
containing phosphors stopped their production (because of liability concerns
) and that is also why you don't see Cd-containing phosphors readily
available for sale any more.

But some of the people who do screen re-coating services, stockpiled some of
the original Cd-containing phosphor because sometimes that is the level of
performance demanded by the customer.

If the phosphor described by Tina was Cd-containing, I really don't think
you want this to be around as an inhalation hazard. I don't know how common
this kind of "oops" has been, but in the event it should happen in your lab,
I would exercise great care to not inhale the particulates and to undertake
a careful clean-up. And while we are on this topic, if you are doing your
own screen coating and using a "lode" of phosphor purchased some years ago,
the chances are very great that it contains Cd, and all precautions should
be taken to avoid inhalation or other exposures when working with the powder



From daemon Thu Apr 06 17:24:33 2000



From: Jorg Wiezorek :      wiezorek+-at-pitt.edu
Date: Thu, 06 Apr 2000 10:01:18 -0400
Subject: TEM - Post-Doc-Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


University of Pittsburgh
Department of Materials Science and Engineering

Postdoctoral Research Position in Transmission Electron Microscopy


A POSTDOCTORAL POSITION has become available in the area of transmission
electron microscopy of thin film data storage media as part of a
collaborative research program between Professors J.M. Wiezorek and W.A.
Soffa, Department of Materials Science and Engineering of the University of
Pittsburgh, and the Seagate Corporation. Candidates should hold a Ph.D. in
Materials Science, Physics or a related discipline and must have extensive
hands-on experience in a broad range of imaging, diffraction and analytical
electron microscopy characterization techniques, as well as with sample
preparation methods. Demonstrated expertise in the preparation of thin film
samples suitable for high-resolution TEM characterization in plan-view and
cross-section is highly desirable. A basic knowledge of magnetism in
materials and experience in instrument development and/or computer image
processing/simulation would be beneficial. The appointment is for one year
in the first instance and is available after June 01/00. Screening of
applications will begin immediately and will continue until the post is
filled. Applications from under-represented groups, including minorities,
women and people with disabilities are encouraged.

Interested candidates should send a curriculum vitae, including publication
list, and the names of at least three referees with postal addresses,
telephone numbers and E-mail addresses to: Prof. J.M. Wiezorek, Department
of Materials Science and Engineering, University of Pittsburgh, 848 Benedum
Hall, Pittsburgh, PA 15261, USA. Email: wiezorek+-at-pitt.edu


________________________________
Jorg M. Wiezorek, Ph.D.
Assistant Professor
Director - Electron Microscopy
Materials Science & Engineering
University of Pittsburgh tel.: 412-624 0122
848 Benedum Hall fax.: 412-624 8069
Pittsburgh, PA 15261, USA



From daemon Thu Apr 06 17:24:35 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Thu, 6 Apr 2000 07:57:39 -0600
Subject: Re: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just for clarification:

The MegaView II is a high-resolution side-mounted TEM camera. It
provides large field of view and high frame rates as well as
high-resolution images. For high-resolution TEM images, as in lattice
images, a bottom-mount camera is more suitable. To that end we have the
BioCam. Although the name seems to imply biological applications, the
name is more a "historical" one and does not reflect the current
applications for the camera.

Nestor, if this sounds like an advertisement, I apologize. Out MegaView
II camera was mentioned in connection with high-resolution images in
materials science, for which it may or may not apply, depending on the
actual requirements.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

Mark YEADON wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} Mike,
}
} You could check out Soft Imaging Systems' MegaView II at:
}
} http://www.soft-imaging-web.de/ {http://www.soft-imaging-web.de/}
}
} I'd like to hear other recommendations too...
}
} Mark
}
} %%%%%%%%%%%%%%%%%%
} Mark Yeadon
} Senior Research Fellow
} Institute of Materials Research and Engineering
} 3 Research Link
} Singapore 117602
}
} Assistant Professor
} Department of Materials Science
} National University of Singapore
} Singapore 119260
}
} TEL: (+65) 874 8591
} FAX: (+65) 872 0785
} Email: m-yeadon-at-imre.org.sg {mailto:m-yeadon-at-imre.org.sg}
}
} -----Original Message-----
} From: Michael Coviello [SMTP:coviello-at-mae.uta.edu]
} Sent: Thursday, March 23, 2000 4:44 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM-Digital camera recommendations
}
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
}
-----------------------------------------------------------------------.
}
} Hi Ya'll:
} We are looking for a CCD camera for our Philips 430 TEM. We
would
} like
} to get the best camera for the best price, e.g., either
buying a
} used
} camera or a new non-Gatan camera (Gatan seems to be twice as
} expensive
} as the others). We would be using the camera for bright field
and
} high
} resolution TEM of materials (semiconductors) rather than for
} biological
} specimens. Does anyone have a camera they would like to
sell/donate
} or
} does anyone have recommendations as to a less-expensive camera
that
} they
} know can be used for materials applications.
} Thanks, Mike Coviello
} Lab Manager
} University of Texas -at- Arlington
}



From daemon Thu Apr 06 17:24:35 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 6 Apr 2000 09:19:36 -0500
Subject: TEM: Immunolabeling on old specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A question for you immuno gurus out there. We have a client who is
interested in looking again at some five-year old blocks, this time in order
to label a protein. The specimens were fixed for standard TEM, i.e., in 2%
glut / 2% paraformaldehyde, followed by osmium. They are embedded in
Epon/Araldite.

I expect that this is going to be a tough one, if it can be done at all. My
question is: are there any secret techniques for optimizing immunogold
labeling in sub-optimal specimens like this? I'm aware of the silver
enhancement methods (although I've never used them). Would that be a good
route to go? Or is it hopeless?

Thanks.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Apr 06 17:24:36 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 6 Apr 2000 09:23:03 -0500
Subject: Horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

Alright, I've watched this string for days now and resisted the temptation,
but it's just too much. So here's mine, before Nestor shuts the string
down. I know several good ones, but I'll restrict this to one on me.

A year ago (recent history!), I was teaching a lab section in Electron
Microscopy, and although overloaded with students, things were cruising
along reasonably well. One day, however, in a fit of brainlessness, I
instructed a student who was doing cell cultures in how to dehydrate samples
for TEM. We were processing his samples in a culture plate. He was going to
embed in Spurr's, so I took him through the ethanol dehydrations up to 100%,
then moved on to, you guessed it, propylene oxide. The plastic cell culture
plate he was using was not amused. Right in front of both of us, it melted
and "embedded" his samples in a resin I was not prepared to risk a diamond
knife on. His comment was something like, "Is it supposed to do that?"

He finished the semester with flying colors and presented a very respectable
project. Even better, we're still friends.

Randy Tindall

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Apr 06 17:24:40 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 6 Apr 2000 10:23:23 -0500
Subject: rotatable stages for inverted scopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone have any good sources for a rotatable stages for inverted
scopes? I would like something with good x & y translation but that
allowed the slide or tray on the stage to rotate while keeping
centered. how do other confocal and deconvolution types solve this
problem? thanks for any tips. Tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Apr 06 17:24:42 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 06 Apr 2000 12:06:45 -0500
Subject: iNFORMATION ON VARIOUS TYPES OF MICROPROBE ANALYSIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Chris MacWilliam wrote:
=========================================================
To whom it may concern:
I am a physics undergraduate and I am currently trying to work on an
assignment in Microscopy. I am trying to find material on the theory and
application of electron beam and x-ray methods of analysis with particular
emphasis on SEM, TEM, EPMA, EDX, WDX, AES and XPS. I was given this website
site as a possible source of information. Any assistance would be greatly
appreciated.

Kindest Regards
Chris MacWilliam.
(ph95ccm-at-brunel.ac.uk)
=========================================================
I would suggest you visit the MICRO 2000 meeting and exhibition next week at
the Novotel London West Hotel. You can get details from the Royal
Microscopical Society website at www.rms.org.uk .

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Thu Apr 06 17:24:42 2000



From: DESHAYEP-at-ch1.ch.pdx.edu
Date: Thu, 6 Apr 2000 9:05:24 PST
Subject: TEM: Ion Tech ion mill needs stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an IonTech (UK manufacture) dual ion thinner which is missing its rotating stage part; this is a
round piece the size of a large washer, with cogs on the perimeter. It is an essential part. Does anyone
know where we can obtain a replacement?

Patrick Deshaye
Geomicrobiology Lab
Portland State University
deshayep-at-ch1.ch.pdx.edu



From daemon Thu Apr 06 17:24:45 2000



From: koyancq3f1nkrz5xza-at-capi.nl
Date: Thu, 06 Apr 2000 12:56:21
Subject: How To Reach Hundreds Of Prospects Every Month

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

S P E C I A L R E P O R T -

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On A Shoestring Budget...

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

Let's face it... obtaining leads and reaching prospects is the
life-blood for today's entrepreneur/small business owner.

The big corporations have traditionally had the advantage in this
area. They have the marketing budget that allows them to flood
the marketplace and get hundreds, if not thousands, of leads.

LEADS TURN TO GOLDEN PROSPECTS

Leads turn into prospects and the if the product or service is
appealing, the result can be a substantial PROFIT!

How can you, the entrepreneur or home based small business
person, level the playing field and compete for your share
without taking out a second mortgage on your house?

THE INTERNET IS THE ANSWER

One area the savvy entrepreneur has turned to is the Internet.

But the Internet is huge. How do you stand out? How do you
generate the leads that are so vital for successful sales?

================================================================
Here's a comparison of Internet/Traditional Advertising Methods:

A Direct Mailing to 250,000 prospects through the Postal Service
will cost you about $150,000.

A Banner Ad that reaches 5 million Internet surfers will cost you
in the neighborhood of $100,000.

Bulk E-Mail to 10 Million people can generate hundreds/thousands
of leads for pennies.
================================================================

.Which Advertising Method is the most economical?

.Which Advertising Method almost guarantee's you'll receive
more leads than you could ever wish for?

================================================================

READ WHAT THE EXPERTS SAY

"Many business people are finding out that they can advertise in
ways that they never could have afforded in the past. The cost
of sending mass e-mail is extremely low, and the response rate is
high and quick." - USA Today.

"E-Mail is an incredible lead generation tool." - Crains Magazine

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It's the tested way to reach thousands of people ... turn leads
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Where do I get the e-mail addresses that will help me contact the
millions of people on the Internet?

HERE'S THE ANSWER:

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We've sold mailing lists for over 5 years and we've never
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This is not a rental list that is restricted to a one-time
mailing. You are purchasing an e-mail address list for your own
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DON'T HESITATE or you will miss out on the least expensive, legal
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95/95/NT or a MAC with SoftWindows.

2. "HOW TO GENERATE LEADS WITH BULK E-MAIL" A manual/guide that
addresses the Bulk E-Mail business. Especially useful for
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For fastest service, you may fax your order to: 1-530-504-7707


To order, via credit card simply cut/paste and print out the
EZ ORDER FORM below and fax to our office today.

***** MILLIONS CD - Volume 9 *****

***** NOW ONLY $247! *****

This "Special Price" is in effect for the next 72 hours,
after that we go back to our regular price of $299.00 ...
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We accept Visa, Mastercard, Amex and Checks by Fax.
Fax your order to: 1-530-504-7707

----------------------Cut & Paste----------------------
---------------------EZ Order Form---------------------

_____Yes! I want everything! I am ordering within 72 hours.
Include ALL bonuses along with my 10 Million E-Mail addresses
(don't forget the 972,565 bonus addresses) at the special price
of only $247.00 + shipping as I have indicated below.

_____Oop's I missed the 72 hour "special". I am ordering my 10
million E-Mail addresses at the regular price of $299.00 +
shipping.

***PLEASE SELECT YOUR SHIPPING OPTION***

____I would like to receive my package FedEx OVERNIGHT. I am
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all other International add an *additional* $25 [$40 total] for
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AVAILABLE for shipments to Alaska, Hawaii, Canada or any
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Address List, the addresses are not rented, but are mine to use
for my own mailing, over-and-over. Free bonuses are included,
but cannot be considered part of the financial transaction. I
understand that it is my responsibility to comply with any laws
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********************************************************
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From daemon Thu Apr 06 17:24:46 2000



From: ancq3f1nkrz5xza-at-neva.spb.ru
Date: Thu, 06 Apr 2000 13:00:29
Subject: How To Reach Thousands Of Prospects With E-Marketing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

S P E C I A L R E P O R T -

How To Generate Hundreds Of Leads Every Day

On A Shoestring Budget...

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

Let's face it... obtaining leads and reaching prospects is the
life-blood for today's entrepreneur/small business owner.

The big corporations have traditionally had the advantage in this
area. They have the marketing budget that allows them to flood
the marketplace and get hundreds, if not thousands, of leads.

LEADS TURN TO GOLDEN PROSPECTS

Leads turn into prospects and the if the product or service is
appealing, the result can be a substantial PROFIT!

How can you, the entrepreneur or home based small business
person, level the playing field and compete for your share
without taking out a second mortgage on your house?

THE INTERNET IS THE ANSWER

One area the savvy entrepreneur has turned to is the Internet.

But the Internet is huge. How do you stand out? How do you
generate the leads that are so vital for successful sales?

================================================================
Here's a comparison of Internet/Traditional Advertising Methods:

A Direct Mailing to 250,000 prospects through the Postal Service
will cost you about $150,000.

A Banner Ad that reaches 5 million Internet surfers will cost you
in the neighborhood of $100,000.

Bulk E-Mail to 10 Million people can generate hundreds/thousands
of leads for pennies.
================================================================

.Which Advertising Method is the most economical?

.Which Advertising Method almost guarantee's you'll receive
more leads than you could ever wish for?

================================================================

READ WHAT THE EXPERTS SAY

"Many business people are finding out that they can advertise in
ways that they never could have afforded in the past. The cost
of sending mass e-mail is extremely low, and the response rate is
high and quick." - USA Today.

"E-Mail is an incredible lead generation tool." - Crains Magazine

E - M A I L M A R K E T I N G W O R K S ! !

It is the most cost effective, low budget, way to gain leads.

It's the tested way to reach thousands of people ... turn leads
into prospects that result in orders that can build wealth for
the entrepreneurial business person.

The big question is:

Where do I get the e-mail addresses that will help me contact the
millions of people on the Internet?

HERE'S THE ANSWER:

M I L L I O N S V O L U M E 9

***New - 10 Million addresses - Just Released***

The cleanest, most comprehensive e-mail address list in the
world, BAR NONE!

We've sold mailing lists for over 5 years and we've never
compromised on quality. We won't release any address list until
it passes our "high standards" test.

This is not a rental list that is restricted to a one-time
mailing. You are purchasing an e-mail address list for your own
personal mailings and may use it over-and-over.

DON'T HESITATE or you will miss out on the least expensive, legal
and most effective way to market... PERIOD!

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

F R E E B O N U S E S

Order the e-mail address list within 72 hours and we'll include
the following FREE Bonuses...

1. To help you get started we include basic proven Professional
Mailing Software. Not a demo, a full working version (SORRY,
SINCE THE SOFTWARE IS FREE WE CANNOT OFFER TECHNICAL SUPPORT,
however set-up instructions are included). Requirement: Windows
95/95/NT or a MAC with SoftWindows.

2. "HOW TO GENERATE LEADS WITH BULK E-MAIL" A manual/guide that
addresses the Bulk E-Mail business. Especially useful for
beginners. "HOW TO GENERATE LEADS WITH BULK E-MAIL" will answer
most of your questions and concerns about Bulk E-Mail. An
exclusive for our customers... INCLUDED FREE.

***SPECIAL BONUS*** Order within the next 72 hours and receive
an additional 972,565 e-mail addresses as a prompt ordering
bonus. Order Now!

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

O R D E R N O W . . . SAME DAY SERVICE (M-F) if your order is
received before 2pm Pacific. If you have further questions or to
place an order, call toll free: 1-800-375-6456 24hours a day.

For fastest service, you may fax your order to: 1-530-504-7707


To order, via credit card simply cut/paste and print out the
EZ ORDER FORM below and fax to our office today.

***** MILLIONS CD - Volume 9 *****

***** NOW ONLY $247! *****

This "Special Price" is in effect for the next 72 hours,
after that we go back to our regular price of $299.00 ...
Don't delay... you can be in business tomorrow!

We accept Visa, Mastercard, Amex and Checks by Fax.
Fax your order to: 1-530-504-7707

----------------------Cut & Paste----------------------
---------------------EZ Order Form---------------------

_____Yes! I want everything! I am ordering within 72 hours.
Include ALL bonuses along with my 10 Million E-Mail addresses
(don't forget the 972,565 bonus addresses) at the special price
of only $247.00 + shipping as I have indicated below.

_____Oop's I missed the 72 hour "special". I am ordering my 10
million E-Mail addresses at the regular price of $299.00 +
shipping.

***PLEASE SELECT YOUR SHIPPING OPTION***

____I would like to receive my package FedEx OVERNIGHT. I am
including $15 for shipping. (Hawaii & Alaska $20 - Canada $25,
all other International add an *additional* $25 [$40 total] for
shipping)

____I would like to receive my package FedEx 2nd Day delivery.
I'm including $10 for shipping. (Sorry FedEx 2nd Day is NOT
AVAILABLE for shipments to Alaska, Hawaii, Canada or any
International destination - Continental U.S. shipping
addresses only).

***Please Print Carefully***

NOTE: Orders cannot be shipped without complete information
including your signature. No exceptions!


NAME____________________________________________________

COMPANY NAME____________________________________________

ADDRESS_________________________________________________
(FedEx can only ship to street addresses - no P.O. boxes)

CITY, STATE, ZIP________________________________________

PHONE NUMBER____________________________________________
(required for shipping & tracking)


EMAIL ADDRESS___________________________________________
(Print Carefully - required in case we have a question and to
send you a confirmation that your order has been shipped)

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From daemon Thu Apr 06 17:24:48 2000



From: Terence Mitchell :      temitchell-at-lanl.gov
Date: Thu, 6 Apr 2000 11:26:04 -0600
Subject: David S. Phillips, 1952-2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David S. Phillips died suddenly of a heart attack on Saturday March
18, while hiking with his son's Boy Scout Group in Bandelier National
Monument, near Los Alamos, New Mexico.

David was born on February 17, 1952, in Columbus, Ohio. Following
graduation from Fairborn High School, Ohio, in 1970, he attended Case
Western Reserve University in Cleveland, Ohio, where he received his
B.S. degree in Metallurgy with highest honors in 1974. He continued
on at Case for his M.S. and Ph.D. degrees researching in Ceramics
with myself and Arthur Heuer. After a post-doctoral appointment with
Jacques Castaing at the CNRS Laboratoire de Physique des Materiaux in
Bellevue, France, David was on the faculty of the University of
Illinois before joining Los Alamos National Laboratory in 1984.

David is survived by his wife Jane and by their sons Sam and Paul,
all of Los Alamos. A memorial fund has been set up in the name of
David Samuel Phillips at Los Alamos National Bank to benefit his sons.

David Phillips was a fine electron microscopist who applied his
skills to a wide variety of problems in ceramics, superconductors,
diamonds and nuclear materials. Amongst his many accomplishments were
seminal papers on the use of high resolution and analytical electron
microscopy to deduce the underlying cause for the star in star
sapphire. He performed beautiful work in science and in everyday life
and will be sorely missed.




xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Terence E. Mitchell
Laboratory Fellow
Materials Science and Technology Division
MS-K765
Los Alamos National Laboratory
Los Alamos, NM 87545
voice mail: 505-667-0938
fax: 505-665-2992
e-mail: temitchell-at-lanl.gov
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Thu Apr 06 17:24:55 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 6 Apr 2000 14:24:30 EDT
Subject: reminder-image analysis short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is still some space left for the North Carolina State University short
course on Image Analysis and Stereology, but you should register soon to
avoid disappointment.

There are two sessions of the workshop, May 11-13 and May 15-17, 2000, at N.
C. State Univ. in Raleigh, NC. This course has been heavily attended and
widely praised for the last 17 years. It offers hands-on training using
computer-based image processing and analysis, couple with practical lectures
by experts in the fields of digital imaging and stereology. Many attendees in
the fields of materials, biology, medical applications, food science,
industrial inspection, and other disciplines involving microscopy have found
this course to be a very practical way to gain understanding and proficiency
in the techniques for acquiring and interpreting structural information.

Full information, including course outlines, faculty information, and on-line
registration, is available at
http://members.aol.com/IPCourse/
You may also contact Cindy Allen at 919 515 8171.



From daemon Thu Apr 06 17:24:58 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 6 Apr 2000 15:59:27 -0400
Subject: TEM: Ion Tech ion mill needs stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Patrick:

VCR Group, Inc. sold Ion Tech products here in the U.S. many years ago.
When we acquired VCR Group last year, we also acquired some old Ion Tech
parts. I will talk to Vince Carlino and check our stock to see if we have
what you need.

Best regards-

David
Writing at 12:11:36 PM on 04/06/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"DESHAYEP-at-ch1.ch.pdx.edu"-at-sparc5.microscopy.com
} -----------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We have an IonTech (UK manufacture) dual ion thinner which is missing its
rotating stage part; this is a
round piece the size of a large washer, with cogs on the perimeter. It is
an essential part. Does anyone
know where we can obtain a replacement?

Patrick Deshaye
Geomicrobiology Lab
Portland State University
deshayep-at-ch1.ch.pdx.edu

{



From daemon Thu Apr 06 17:24:58 2000



From: David Bentley :      dlb-at-u.arizona.edu
Date: Thu, 06 Apr 2000 13:08:08 -0700
Subject: Horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Haven't seen this one submitted to the list yet, but this is a nightmare
we all have.
Another department on campus was kind enough to give us a TEM, a JEOL
100CXII. It, of course, had minor vacuum leak which put it out of service,
but besides that it was in mint condition. I had planned to move it
myself but was overruled by my boss. There was concern for injury to
myself or others in case it fell. I begged, whined, pleaded, and lost the
discussion. A moving company would transport. I talked to the
administrative person arranging the move and was told that the moving
company said it had all the information it needed. Bells, whistles,
lights, and red flags went off in my mind. I finally got hold of the
moving company supervisor and was told he had done things like this before
and knew what had to be done. I told him several times, NOT to use the
liftgate on his moving truck, to use a forklift. The column was too heavy,
and balanced wrong for liftgates to work well. He assured me HIS truck
could easily lift the 3,000 lbs. More flags waved! I told him one last
time to use a forklift to avoid problems. The day for moving came and this
day, as well as the next two appointments, the movers gave the excuse the
truck clutch was out, more red flags. When finally they did get the clutch
repaired, I unfortunately (fortunately?) was not there to see the
fireworks, they tried to use the lift gate, got the column several inches
off the ground before both hydraulic cylinders for the truck lift gate
sheared off, dropping the gate and giving the column a good bounce. As
good luck would have it, the microscope didn't tip over, just wobbled a
lot. Had it been higher, a lot of damage could have occurred as well as
someone getting hurt. To top this story off, the moving company had the
audacity to try to charge the University for the cost of repairing their
truck. The damage to the microscope was limited to knocking the
intermediate and projector lenses out of alignment and the instrument is
working wonderfully in its new home. "Takes a lickin' and keeps on tickin'."
Later;
David L Bentley
Imaging Facility
The University of Arizona
Tucson, AZ 85721-0036
(520)621-5097



From daemon Thu Apr 06 17:47:26 2000



From: Ed Kurz :      ekurz-at-mail.ims.uconn.edu
Date: Thu, 6 Apr 2000 17:32:18 -0500
Subject: Micro-Raman

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would this group be a good place to post micro-Raman questions or is there
a better group?

We are just beginning to use this technique. Can anyone recommend a
computer reference library for Raman? How do different wavelength lasers
affect frequency shift and amplitudes?

Thanks,
Ed


Ed Kurz
Institute of Materials Science, U3136
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269-3136
ekurz-at-mail.ims.uconn.edu
(860) 486-4186 phone
(860) 486-4745 fax




From daemon Thu Apr 06 17:55:10 2000



From: vriesg-at-rpi.edu ()
Date: Thu, 6 Apr 2000 17:46:23 -0500
Subject: Re: application of atomic force microscopy

Contents Retrieved from Microscopy Listserver Archives
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Email: vriesg-at-rpi.edu
Name: Gwynne Vriesema
School: Rensselaer Polytechnic Institute

Question: I am doing a project on the application of atomic force
microscopy to making read/write devices that have
a much higher data density than today's magnetic
drives. Do you see any drawbacks to using AFM for
this application? What aspect of AFM is most
important to understand when figuring out how these
drives would work? What are the advantages to using
this method to store data? Thank you for your time!

---------------------------------------------------------------------------




From daemon Thu Apr 06 18:37:07 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 6 Apr 2000 19:21:01 -0400
Subject: "S" -distortion in TEM images JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
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I have a JEOL 2000FX and I am getting an "S" distortion in my images at all
mags. The severity differs slightly with mag. I know that the distortion
is there because I am looking at layered structures on Si. I put the
samples with the interfaces parallel to the rod axis. As I move the sample
along the rod axis, the "s" stays in the same place. I looked at the
Kikuchi lines in an on-axis CBED pattern. They do not seem to suffer the
same problem, but there is a little at low camera lengths at the outside
parts of the pattern.

Does anyone know the source of this distortion and is there any cure for it?

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Thu Apr 06 20:15:12 2000



From: James Martin :      James.S.Martin-at-williams.edu
Date: Thu, 06 Apr 2000 21:05:09 -0400 (EDT)
Subject: Re: Micro-Raman

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I've operated a listserv for a couple of years called Irusers-l, which has
been used by scientists who work in museums and laboratories that
use FTIR or micro-FTIR to analyze historic and artistic works. Some
members also use Raman. We've had brief discussions about expanding the
group to any scientist who uses FTIR or Raman, regardless of application.

Would members of this list be interested in joining Irusers-l?

James Martin

Research Scientist in Chemistry
Williams College
james_martin.tripod.com/williams.htm

Director
Orion Analytical, LLC

On Thu, 6 Apr 2000, Ed Kurz wrote:

}
} Would this group be a good place to post micro-Raman questions or is there
} a better group?
}
} We are just beginning to use this technique. Can anyone recommend a
} computer reference library for Raman? How do different wavelength lasers
} affect frequency shift and amplitudes?
}
} Thanks,
} Ed
}
}
} Ed Kurz
} Institute of Materials Science, U3136
} 97 North Eagleville Road
} University of Connecticut
} Storrs, CT 06269-3136
} ekurz-at-mail.ims.uconn.edu
} (860) 486-4186 phone
} (860) 486-4745 fax
}
}
}
}




From daemon Thu Apr 06 20:34:34 2000



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Thu, 06 Apr 2000 21:29:13 -0400
Subject: Microscopy horror stories

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists -

Waaay back, when I was an instructor at MIT in Cambridge, Mass., I was
helping out in the physical metallurgy laboratory course when we were
teaching the students how to develop glass metallographic plates (told
you it was a long time ago). One of my students, the son of a famous
metallurgist, placed his fully developed plate on the belt of a machine
that he didn't notice was running. We never let him forget the day he
tried to dry his plate in the cylindrical print drier.

George Langford, Sc.D., in PA trying to forget that everyone used
mercury ice-point reference junctions in their thermocouples in the
heat-treating lab in those days ... and how many got tipped over.



From daemon Thu Apr 06 20:54:27 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Thu, 6 Apr 2000 21:51:13 -0400 (EDT)
Subject: seeking recommendation for stereo zoom

Contents Retrieved from Microscopy Listserver Archives
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I have a colleague with advanced presbyopia who is doing consulting work
and needs a stereo zoom to do botanical identification. He wants to stay
below US $1,000.

He has found the following scopes on the web:

TT-5Z and TT-5B at the www.ken-a-vison.com web site

SMZ Bino zoom at www.microscope.org

Any comments on any of these or recommendations for other scopes in his
price range.

Thank you for any good suggestions.

Don

(Direct replies from vendors are welcomed, but please identify your
financial interest in your offers/recommendations.)



______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Fri Apr 07 07:59:32 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 7 Apr 2000 13:05:12 +1000
Subject: folds in semi-thin resin sections

Contents Retrieved from Microscopy Listserver Archives
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Can you etch the sections with ethoxide (Ehanol + NaOH)? This has
always been my way around the problem for Epon sections.

Diana
--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Fri Apr 07 07:59:47 2000



From: richard.beanland-at-gecm.com
Date: Fri, 07 Apr 2000 09:12:09 +0000 (GMT)
Subject: Re: TEM: Ion Tech ion mill needs stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Patrick,
Ion Tech changed their name to Atom Tech a few years ago. They can be reached at:

Island Farm Avenue,
West Moseley,
Surrey,
KT8 2UZ
UK

Tel (+44) 181 941 8959
Fax (+44) 181 941 8948
http://www.atomtech.co.uk/

They will supply replacement parts for most of their machines. (While you're about it, get more ceramic resistors, Al cathodes, cathode stops etc.) If you're rejuvenating one of these machines I'd be happy to describe the maintenance and alignment procedures - I have been taking these things to bits and reassembling them for a few years now.

Cheers,


Richard Beanland

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} We have an IonTech (UK manufacture) dual ion thinner which is missing its rotating stage part; this is a
} round piece the size of a large washer, with cogs on the perimeter. It is an essential part. Does anyone
} know where we can obtain a replacement?
}
} Patrick Deshaye
} Geomicrobiology Lab
} Portland State University
} deshayep-at-ch1.ch.pdx.edu
}



From daemon Fri Apr 07 07:59:53 2000



From: John Minter :      minter-at-kodak.com
Date: Fri, 7 Apr 2000 07:23:21 -0400
Subject: Yet another horror story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shortly after our laboratory purchased our first analytical electron
microscope, the microscope developed a problem in the electronics.
The microscopist telephoned the vendor's service department who requested
he measure some voltages to help diagnose the problem. The microscopist
dutifully attached clip-leads to the appropriate test points and was
attaching them to the voltmeter when one of the clip-leads slipped off,
fell into the electronics chassis, and shorted out much of the electronics.
The vendor service ingineers were in the lab for weeks repairing the
problem. The rest of the lab teased him about this, giving the the
microscopist the nickname, "clip-lead". A few years later when he
left the group for a promotion to a staff job, he was presented with
a pair of clip-leads where the metallic ends had been coated with
liquid-rubber.

Best Regards,

John Minter
Eastman Kodak Company
Analytical Technology Division
Rochester, NY 14652-3712
Phone: (716) 722-3407
FAX: (716) 477-3029
email: john.minter-at-kodak.com



From daemon Fri Apr 07 07:59:56 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Fri, 7 Apr 2000 22:13:22 +1100
Subject: RE: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's one that involved the fire department:

Early one evening when the only people still in the building were us
students, a guy from along the corridor came into our room saying that he
could smell something downstairs. We all banded together and went down to
investigate. After a while we noticed this sort of thick foggy smoke
beginning to form below the ceiling along the length of the corridor and in
the adjacent large teaching lab. We noticed it wafting down through some
light fittings and gradually getting lower down and thicker. "Has to be
electrical!" "Looks like the whole place could go up any minute!" It
started looking pretty serious. When the fire brigade turned up we were
told to get out as they proceeded to rip out chunks of the ceiling trying
to locate the source. It was a very diffuse source and there was a very
large area of ceiling to rip chunks out of. After a while their
investigation moved upstairs -- where they found our carbon coater happily
chugging away with it's bell jar off and the exhaust line feeding down into
the ceiling cavity! You learn something new every day.

I think we remembered to say thanks....








Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Fri Apr 07 07:59:59 2000



From: Graham.Cheeseman-at-acadiau.ca
Date: Fri, 7 Apr 2000 09:51:23 -0300
Subject: TM SM Surplus Equipment for Donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{paraindent} {param} left {/param} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Times New Roman {/param} {bigger} The Department of Biology, Acadia University, Wolfville,
NS have two electron microscopes for disposal. The first is
a Zeiss EM9A transmission electron microscope and the
second a JEOL JSN-25S scanning electron microscope.
Both microscopes are operable, but are surplus to the
current needs of the department. The department is willing
to donate these instruments to an institution, providing that
institution is willing to assume the responsibility for removal
and shipping to the new destination. For further information
contact Dr. T.B. Herman (902) 585-1469. {/paraindent}


{nofill}



==============================================================
Graham N. Cheeseman
Biology Dept., Acadia University
Wolfville, Nova Scotia
B0P 1X0
TEL: (902) 585-1316
FAX: (902) 585-1059
INTERNET: Graham.Cheeseman-at-acadiau.ca
==============================================================

{/x-rich}



From daemon Fri Apr 07 17:49:38 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Fri, 07 Apr 2000 08:39:18 -0500
Subject: Cold Horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hope this scary one helps some of you!
We are a research and teaching lab (thankfully!) A very lucky (for us)
student was in working on a weekend when she noticed white smoke pouring
out of the lab into the lecture room, grabbed the extinguisher, and put out
the electrical fire that started in the wall due to our specimen rotator
motor shorting out (and not being properly wired). The fire was directly
underneath the 1 gallon storage tanks for 100% ethanol, and pure
Xylene..... {yipe} ! So bad, that it melted but did not break through the
spigots... {big yipe} !
I also learned that when your filling a liquid nitrogen tank, and it gets
full, and it begins to shoot up a little underneath a temperature sensor,
you get to meet the fire chief!
Life is good!





Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Fri Apr 07 17:49:39 2000



From: rlvaughn-at-unmc.edu
Date: Fri, 7 Apr 2000 08:49:48 -0500
Subject: Re: TEM: Immunolabeling on old specimens

Contents Retrieved from Microscopy Listserver Archives
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Hi Randy
One good "old" reference is Moise Bendayan and Max Zollinger.
Ultrastructural Localization of Antigenic Sites on Osmicated-fixed Tissues
Applying the Protein A-Gold Technique. J Histochem Cytochem 31 :101-109,
1983
Some times it works some times it doesn't. I would like to see some newer
tricks if they are out there.

Rick Vaughn



From daemon Fri Apr 07 17:49:42 2000



From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 7 Apr 2000 10:12:46 -0400
Subject: Horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My horror story happened many years ago when I came into work one morning to
find the Philips200 had been left running for a long period of time with no
water coursing through its veins. The column was hot to touch so my
immediate reaction was to turn on the water supply to try to cool it down.
But hoses and o-rings had deteriorated from the heat, so instead of cooling
the instrument down I now had water gushing from everywhere and filling up
the columnâs viewing area so that it looked like a fish bowl. I called my
Philips service engineer and he spent the next 3 days repairing and cleaning
up the scope. He said the stage had come very close to a melt down and if
that had happened I could have just dug a hole and buried the scope in the
back yard. The scope survived to give the lab many years of service, thanks
to Philips engineer, John Braunagel, whose expert service was given without
a grumble or complaint.




From daemon Fri Apr 07 17:49:48 2000



From: Joyce Craig :      j-craig-at-csu.edu
Date: Fri, 07 Apr 2000 10:10:02 -0500
Subject: horror

Contents Retrieved from Microscopy Listserver Archives
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I set up an lab in a hospital with a Siemens microscope-a wonderful
instrument for resolution but a beast to align. The lenses were
physically moved during alignment so that it did look odd to see
different components of the column a few centimeters off. One morning I
came in and a pathologist proudly told me that he had aligned the column
for me. He had straightened it out very nicely and it took a full day
for me to get it back in alignment. It did look nicer the way he did it
but of course it was impossible to use.
Joyce Craig
Chicgo State University



From daemon Fri Apr 07 17:49:48 2000



From: Joyce Craig :      j-craig-at-csu.edu
Date: Fri, 07 Apr 2000 10:12:53 -0500
Subject: sputter coater

Contents Retrieved from Microscopy Listserver Archives
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How long should a gold target last?
Our target looks gold except in the center.
Problem is that we don't seem to get good coating.
Joyce Craig
Chicago State University



From daemon Fri Apr 07 17:49:50 2000



From: Betty Loraamm (BIO) LIF136 :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 7 Apr 2000 11:53:32 -0400 (EDT)
Subject: Horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My horror story happened many years ago when I came into work one morning
to find the Philips200 had been left running for a long period of time
with no water coursing through its veins. The column was hot to touch so
my immediate reaction was to turn on the water supply to try to cool it
down. But hoses and o-rings had deteriorated from the heat, so instead of
cooling the instrument down I now had water gushing from everywhere and
filling up the column's viewing area so that it looked like a fish
bowl. I called my Philips service engineer and he spent the next 3 days
repairing and cleaning up the scope. He said the stage had come very
close to a melt down and if that had happened I could have just dug a hole
and buried the scope in the back yard. Surprisingly the scope survived to
give the lab many years of service, thanks to Philips service engineer
John B's expert service that he gave without a grumble or complaint.



From daemon Fri Apr 07 17:49:54 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 7 Apr 2000 12:17:14 -0400
Subject: A funny 'horror' story

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This is a story that was told to me by Dr. Audrey Glauert of Cambridge
University in the U.K. Even though it's not my own, it is so amusing that
I can't help relaying it to you.

It seems that a number of years ago Dr. Glauert spent several months
working in an electron microscopy laboratory in Africa. Every now and then
the water supply to the laboratory would go off making it necessary to shut
the electron microscope down for an extended period of time. Investigation
eventually revealed that the problem arose because the town involved was
getting it's water from a pond that was formed behind a dam that had been
constructed across a nearby river. It seems that this pond was a favorite
site for a herd of hippopotamuses to bathe, and every now and then one of
them would manage to plug up the water inlet to the village water system,
whereupon it was necessary for the villagers to go out and chase the hippos
away and then open the inlet again. Since hippos are not very easy to
chase, this could sometimes cause a rather prolonged period without water
in the electron microscopy laboratory.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Apr 07 17:49:56 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 7 Apr 2000 14:05:53 -0400 (Eastern Daylight Time)
Subject: Re: Microscopy horror stories

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With all these horror stories being shared, its surprising that nobody
has related a "darkroom" tale yet. Surely everyone who has been in
this field for several years has had the unpleasant experience of
walking into the darkroom and finding everything coated with dried
photographic fix residue? My darkroom experiences with users have been
all in all much worse that microscope incidents.

WL Steffens
University of Georgia
Department of Pathology



From daemon Fri Apr 07 17:50:01 2000



From: Graham.Cheeseman-at-acadiau.ca -at-sparc5.microscopy.com
Date: Fri, 7 Apr 2000 09:51:23 -0300
Subject: TM SM Surplus Equipment for Donation

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{paraindent} {param} left {/param} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Times New Roman {/param} {bigger} The Department of Biology, Acadia University, Wolfville,
NS have two electron microscopes for disposal. The first is
a Zeiss EM9A transmission electron microscope and the
second a JEOL JSN-25S scanning electron microscope.
Both microscopes are operable, but are surplus to the
current needs of the department. The department is willing
to donate these instruments to an institution, providing that
institution is willing to assume the responsibility for removal
and shipping to the new destination. For further information
contact Dr. T.B. Herman (902) 585-1469. {/paraindent}


{nofill}



==============================================================
Graham N. Cheeseman
Biology Dept., Acadia University
Wolfville, Nova Scotia
B0P 1X0
TEL: (902) 585-1316
FAX: (902) 585-1059
INTERNET: Graham.Cheeseman-at-acadiau.ca
==============================================================


{/x-rich}



From daemon Fri Apr 07 17:50:04 2000



From: Vetrano, John S :      john.vetrano-at-pnl.gov
Date: Fri, 07 Apr 2000 14:00:12 -0700
Subject: Horror: Why my post doc was almost cut short

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About a month after starting my post doc in the lab of Professor Ruhle in
Stuttgart, Germany, I was working on the TEM late one Sunday night. I was using
a double-tilt, analytical, double-specimen holder (read: expensive) and just
putting it away. The holder was gripped in a Gatan holder stand and when I
pushed the protective end sheath onto the tip the stage moved back so the front
stand grip moved onto the narrower part of the holder where it doesn't grip.
The motorized back end was heavy and the holder tipped backwards while I was
still holding the protective end piece firm. What resulted is a holder that
greatly resembled a Concord airplane coming in for a landing. It bent the
holder at about a 30 degree angle right where the hinge was for the back
specimen cup.

I sat and looked at it, beads of sweat forming on my forhead, contemplating the
fact that I had an open ended return ticket and wondering if I could pack my
stuff up and be gone back to the U.S. before anyone noticed. Of course I stayed
and Professor Ruhle was very good about it, basically saying that mistakes
happen but don't do it again! I didn't.

Lessons Learned: Be very careful when handling expensive specimen holders;
think twice before you do anything.

I hope you all have a good weekend and don't mind my confessions!

Cheers, JSV

***************************
John Vetrano
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0724 Fax: (509)376-6308
Email: mailto:john.vetrano-at-pnl.gov



From daemon Fri Apr 07 17:50:08 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 07 Apr 00 16:39:01 -0600
Subject: Horror Prevention:

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Maybe Judy Murphy's Delta College will diminish these "untrained"
occurrences!

Bernie Kestel
Argonne National Laboratory



From daemon Fri Apr 07 17:50:08 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Fri, 7 Apr 2000 14:55:56 -0700 (PDT)
Subject: Mat Sci:Need Tips for Ion Milling Porous Ceramic

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If anyone has any tips or a reference they could point me to for this, it
would be much appreciated.

We have had several users with nanocrystalline ceramic samples from
combustion or plasma syntheses who have had problems with the thin areas
of their samples falling out during ion milling. We have tried a few
schemes to correct this, without much success. Any help would be greatly
appreciated.

The current user has 2 different nanocrystalline composites: TiB2/TiN and
TiB2/TiC.

Valerie Leppert
Dept. Chem. Eng. and Mat. Sci.
University of California, Davis

vjleppert-at-ucdavis.edu



From daemon Fri Apr 07 17:50:09 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 7 Apr 2000 17:46:00 -0500
Subject: Re:sputter coater

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That is a difficult question since it depends on so many variables (original
target thickness, coating rates, time run, etc). I use my sputter coater
often
(but not as often as the carbon evaporator). Never kept accurate records of
usage, but it is at least several years old.

In any event, the entire target should have a bright gold color. Some areas
may
have a matte vs shiny appearance, but should not be dark. If not all bright
Au,
it is either consumed or contaminated. Either case will cause sputtering to
cease.

Woody White




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How long should a gold target last?
Our target looks gold except in the center.
Problem is that we don't seem to get good coating.
Joyce Craig
Chicago State University



From daemon Fri Apr 07 17:50:09 2000



From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 7 Apr 2000 10:12:46 -0400
Subject: Horror stories

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My horror story happened many years ago when I came into work one morning to
find the Philips200 had been left running for a long period of time with no
water coursing through its veins. The column was hot to touch so my
immediate reaction was to turn on the water supply to try to cool it down.
But hoses and o-rings had deteriorated from the heat, so instead of cooling
the instrument down I now had water gushing from everywhere and filling up
the columnâs viewing area so that it looked like a fish bowl. I called my
Philips service engineer and he spent the next 3 days repairing and cleaning
up the scope. He said the stage had come very close to a melt down and if
that had happened I could have just dug a hole and buried the scope in the
back yard. The scope survived to give the lab many years of service, thanks
to Philips engineer, John Braunagel, whose expert service was given without
a grumble or complaint.





From daemon Fri Apr 07 17:50:11 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 07 Apr 2000 15:35:17 -0500
Subject: The SCSI interface saga update

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There was some discussion about scanners and SCSI bus
problems. There are some recent developments that I'd
like to share with those who are having SCSI-related problems.

If you have a PC which includes one or two USB ports, there
is a new converter cable which should make your life much
better. Microtech has a USB to SCSI-II converter cable
(# USB-SCSI-HD50) that plugs into a USB socket and provides
a high density 50 pin SCSI-II plug. This plug can be converted
to SCSI-I wide Centronics ribbon-style connector if necessary.
This converter cable works with PCs and Macs; and so far, all
of my SCSI devices work with it (scanners, CD-R, CD-RW).

This item is available from various sources for about $80.
I got mine from d-store.com but you might find other sources.

Hope this helps those having SCSI problems.

gary g.



From daemon Fri Apr 07 17:50:11 2000



From: Cynthia Shannon :      cshannon-at-nctimes.net
Date: Fri, 07 Apr 2000 15:24:09 -0700
Subject: Wanted:Used Zeiss 10c TEM

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a functioning Zeiss 10c TEM that they would like to
donate to a goverment office for a tax deduction or sell for cheap?
I am the electron microscopist for the county veterinarian and we are
trying to locate a Zeiss 10c to be used for virus identification work on
animals and plants.
Thank you.
Cindy Shannon
cshannon-at-nctimes.net



From daemon Sat Apr 08 08:03:09 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 07 Apr 2000 19:47:18 -0700
Subject: Re: application of atomic force microscopy

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Gwynne,

Suggest you visit the sites for both Digital Instruments and
ThermoMicroscopes. Both have application notes which will be helpful.
Also, suggest that you contact Sergei Magonov at DI and Jezz Leckenby at
ThermoM. Both are extremely knowledgeable apps specialists.
Sergei: 805-967-1400
Jezz: (408) 747-1600

Hope this is helpful.

Caveat: MME has no financial interest in either of these systems.
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 05:46 PM 4/6/00 -0500, wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Apr 08 08:03:13 2000



From: Terje Dokland :      dokland-at-ima.org.sg
Date: Sat, 8 Apr 2000 10:06:50 +0800
Subject: TEM - Technician position available at IMA, Singapore

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ELECTRON MICROSCOPIST
Laboratory of Structural Biology
Institute of Molecular Agrobiology, Singapore

A position is available for an experienced EM technician at the
Institute of Molecular Agrobiology (IMA), Singapore, in the group of Dr.
Terje Dokland, Laboratory of Structural Biology. The successful candidate
will be responsible for the operation of the structural EM laboratory, and
be involved in several research projects, in particular structural
characterization of various plant and animal viruses of agricultural
importance. Experience with cryo-EM of frozen-hydrated samples is a
definite advantage, as is some experience with using computers, especially
3D reconstruction methods.
IMA is a well funded and rapidly expanding research institute which
was established in 1995 to conduct basic and applied research in
agrobiology. It moved into a modern and spacious building at the National
University of Singapore campus two years ago, and has state-of-the art
equipment for structural and molecular biology, biochemistry, plant growth,
tissue culture etc. There are currently two groups working in structural
biology, including X-ray crystallography and electron microscopy, and there
is extensive collaboration with other laboratories and institutes, both in
Singapore and abroad. Further information about the institute can be found
at http://www.ima.org.sg. Singapore is a cosmopolitan and multicultural
society offering all the conveniences of a modern city, and is centrally
located in Southeast Asia with easy access to other countries in the
region.
The position will be filled at the Research Officer or Assistant
Research Officer level, depending on experience, and the contract will be
initially offered for two years. IMA offers attractive salaries with bonus
packages and benefits.
Informal enquiries are welcome and can be directed to Terje Dokland
at dokland-at-ima.org.sg. Applications, including a CV and names of two
referees, should be sent to
Terje Dokland
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604


------------------------------------
Terje Dokland
Senior Scientist
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604
Phone: 65-872 7405 Fax: 65-872 7007
E-mail: dokland-at-ima.org.sg

"Convictions are greater enemies of truth than lies"
F. Nietzsche




From daemon Sat Apr 08 08:03:18 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Fri, 7 Apr 2000 14:55:56 -0700 (PDT)
Subject: Mat Sci:Need Tips for Ion Milling Porous Ceramic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone has any tips or a reference they could point me to for this, it
would be much appreciated.

We have had several users with nanocrystalline ceramic samples from
combustion or plasma syntheses who have had problems with the thin areas
of their samples falling out during ion milling. We have tried a few
schemes to correct this, without much success. Any help would be greatly
appreciated.

The current user has 2 different nanocrystalline composites: TiB2/TiN and
TiB2/TiC.

Valerie Leppert
Dept. Chem. Eng. and Mat. Sci.
University of California, Davis

vjleppert-at-ucdavis.edu




From daemon Sat Apr 08 08:03:18 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 07 Apr 00 16:39:01 -0600
Subject: Horror Prevention:

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Maybe Judy Murphy's Delta College will diminish these "untrained"
occurrences!

Bernie Kestel
Argonne National Laboratory




From daemon Sat Apr 08 08:03:19 2000



From: Cynthia Shannon :      cshannon-at-nctimes.net
Date: Fri, 07 Apr 2000 15:24:09 -0700
Subject: Wanted:Used Zeiss 10c TEM

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Does anyone have a functioning Zeiss 10c TEM that they would like to
donate to a goverment office for a tax deduction or sell for cheap?
I am the electron microscopist for the county veterinarian and we are
trying to locate a Zeiss 10c to be used for virus identification work on
animals and plants.
Thank you.
Cindy Shannon
cshannon-at-nctimes.net




From daemon Sat Apr 08 08:03:19 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Fri, 07 Apr 2000 23:58:40 -0400
Subject: Perfluorinated Polyether Oils and Greases -- are they a problem ?

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This is actually a follow-on question to the thread on "Silicone Oils and
Greases" which ran a few days ago.

In his typically thorough response to the discussion of why microscopists
generally avoid silicone-based vacuum greases, Will Bigelow noted that

} While the silicone high vacuum grease is
} indeed a good lubricant for O-rings, it is no better than the Brayco and
} Krytox greases, which are based on polyphenylether compounds, and which do
} not introduce the possibility of having insoluble siliceous compounds
} formed on critical parts of the electron optical column.
}

This mention of Krytox rang a bell and I checked Chapter 10 of Will's book
"Vacuum Methods in Electron Microscopy" where he discusses oils and greases.
In table 10.1 he lists both Braycote and Krytox as "Perfluorinated Polyether
based" (not polyphenylether). In the subsequent discussion he states that they
"probably should not be used ... in or near the electron gun because of the
possibility that some of the perfluorinated base compound might get onto the
high voltage insulator and cause microdischarges ... ". In chapter 5 he is
more specific in his discussion of perfluorinated polyether diffusion pump oils
where he again mentions Krytox and states that use of these pump oils was
generally abandoned because it was found that "electron microscopes in which
these fluids were used eventually developed high-voltage instabilities due to
micro-discharges along the ceramic insulators in the electron guns." Will goes
on to note that the problem seems to be more severe in TEMs (which operate at
higher voltages) and these fluids "... have been used successfully for several
years in scanning electron microscopes in some laboratories".

First question:
I'm confused by Will's list-server statement that Krytox is a polyphenylether
when it is listed in his book as a perfluorinated polyether. Not being an
organic (or any other kind) of chemist, I might think that these are synonyms
for the same family of materials except that Will also has a separate
discussion of polyphenyl ether pump fluids (e.g., Convalex and Santovac) which
is separate from that of the perfluorinated polyethers (Krytox and Fomblin). I
think that Krytox actually is a perfluorinated polyether, isn't it?

Second question:
The issue of whether Krytox and Braycote are perfluorinated polyethers is more
than a trivial nomenclature issue because of the alleged problem of
perfluorinated compounds contaminating high-voltage insulators. In addition to
the somewhat equivocal cautionary statements in Will's book, I personally know
of one lab which has banished these compounds from the premises because of an
incident a number of years ago where the glassware used for cleaning of
microscope parts got contaminated with Krytox -- this got transferred to
several TEMs and resulted (so I'm told) in the need to replace multiple guns
over a period of time -- and produced a lot of hair-pulling until the source of
the problem was identified (actually, maybe this should be under the "horror
stories" thread?). But this is the only direct report I have heard of such a
problem and Will, though he notes the concerns, doesn't seem reluctant to
recommend the stuff for EM use (and I do respect his depth of experience in
such things). So my question: Is Krytox really the "bogeyman" I've been told
it is? Or is this just another bit of microscopy folklore?

Given that: (1) there are lots of ways a vacuum grease could get transferred to
a gun indirectly (i.e., via tools or contaminated gloves); (2) this stuff is
reported to be essentially impossible to remove once it gets deposited on
something; (3) the purported HV discharge behavior doesn't show up immediately
but develops gradually over time; and (4) insulator cleanliness is enough of a
problem without introducing this kind of sneaky contaminant -- IF true, it
would seem that this class of compounds has no place in an EM lab. But if this
is all a myth, I'm depriving myself of some otherwise great products. Insight
anyone?

Fred Schamber



From daemon Sat Apr 08 08:03:28 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:22:26 +0100
Subject: Re: sputter coater

Contents Retrieved from Microscopy Listserver Archives
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Hi

Most sputter coater manufacturers use one of two methods for mounting their
targets.

1. Target material fixed by a clip or adhesive to an aluminium base
(difficult to sputter without a special coater)

2. Target material fixed by adhesive to a brass base (this will sputter
in relation to its composition e.g. 70%Cu 30%Zn)

You seem to have the type (2) target and are probably sputtering a mixture
of gold (from the outside of the target) and brass (from the middle of the
target). Check it out if you have x-ray analysis?

Sputter targets last a period in direct relationship to target thickness,
coating thickness and degree of use, how long is a piece of string?

Good luck

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com




From daemon Sat Apr 08 08:03:28 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:21:38 +0100
Subject: Re: "S" -distortion in TEM images JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

"S" distortion or anisotropic distortion is produced through a balancing of
aberrations. Take one imaging lens which is being used at low current and
you will have pincushion distortion. Add another lens that is being used in
the low to middle part of its current range and you will have pincushion
distortion. Use the two lenses in a magnification system and the result is
an image with anisotropic distortion.

When we design a TEM we try to balance the lenses so that the degree of
distortion is minimised. Minimised does not mean totally removed it means
NO distortion in the area that falls within the photographic frame. In
early instruments, where the screen was very small, you hardly ever saw the
problem, as screens have increased in size so the problem becomes more
clear.

If the distortion has just started to become annoying this may be due to one
or two reasons.

1) The high voltage has changed such that the lenses are not matched to
the accelerating voltage as they were when the system was new?

2) A lens is not running at the correct level, its current is too low?

The most common reason for a change in lens or high voltage performance are
their reference circuits, I am afraid I am not familiar with these circuits
in the 2000FX.

Good luck hope this helps?

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com




From daemon Sat Apr 08 08:03:32 2000



From: Terje Dokland :      dokland-at-ima.org.sg
Date: Sat, 8 Apr 2000 10:06:50 +0800
Subject: TEM - Technician position available at IMA, Singapore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ELECTRON MICROSCOPIST
Laboratory of Structural Biology
Institute of Molecular Agrobiology, Singapore

A position is available for an experienced EM technician at the
Institute of Molecular Agrobiology (IMA), Singapore, in the group of Dr.
Terje Dokland, Laboratory of Structural Biology. The successful candidate
will be responsible for the operation of the structural EM laboratory, and
be involved in several research projects, in particular structural
characterization of various plant and animal viruses of agricultural
importance. Experience with cryo-EM of frozen-hydrated samples is a
definite advantage, as is some experience with using computers, especially
3D reconstruction methods.
IMA is a well funded and rapidly expanding research institute which
was established in 1995 to conduct basic and applied research in
agrobiology. It moved into a modern and spacious building at the National
University of Singapore campus two years ago, and has state-of-the art
equipment for structural and molecular biology, biochemistry, plant growth,
tissue culture etc. There are currently two groups working in structural
biology, including X-ray crystallography and electron microscopy, and there
is extensive collaboration with other laboratories and institutes, both in
Singapore and abroad. Further information about the institute can be found
at http://www.ima.org.sg. Singapore is a cosmopolitan and multicultural
society offering all the conveniences of a modern city, and is centrally
located in Southeast Asia with easy access to other countries in the
region.
The position will be filled at the Research Officer or Assistant
Research Officer level, depending on experience, and the contract will be
initially offered for two years. IMA offers attractive salaries with bonus
packages and benefits.
Informal enquiries are welcome and can be directed to Terje Dokland
at dokland-at-ima.org.sg. Applications, including a CV and names of two
referees, should be sent to
Terje Dokland
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604


------------------------------------
Terje Dokland
Senior Scientist
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604
Phone: 65-872 7405 Fax: 65-872 7007
E-mail: dokland-at-ima.org.sg

"Convictions are greater enemies of truth than lies"
F. Nietzsche





From daemon Sat Apr 08 08:18:20 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Fri, 07 Apr 2000 23:58:40 -0400
Subject: Perfluorinated Polyether Oils and Greases -- are they a problem ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is actually a follow-on question to the thread on "Silicone Oils and
Greases" which ran a few days ago.

In his typically thorough response to the discussion of why microscopists
generally avoid silicone-based vacuum greases, Will Bigelow noted that

} While the silicone high vacuum grease is
} indeed a good lubricant for O-rings, it is no better than the Brayco and
} Krytox greases, which are based on polyphenylether compounds, and which do
} not introduce the possibility of having insoluble siliceous compounds
} formed on critical parts of the electron optical column.
}

This mention of Krytox rang a bell and I checked Chapter 10 of Will's book
"Vacuum Methods in Electron Microscopy" where he discusses oils and greases.
In table 10.1 he lists both Braycote and Krytox as "Perfluorinated Polyether
based" (not polyphenylether). In the subsequent discussion he states that they
"probably should not be used ... in or near the electron gun because of the
possibility that some of the perfluorinated base compound might get onto the
high voltage insulator and cause microdischarges ... ". In chapter 5 he is
more specific in his discussion of perfluorinated polyether diffusion pump oils
where he again mentions Krytox and states that use of these pump oils was
generally abandoned because it was found that "electron microscopes in which
these fluids were used eventually developed high-voltage instabilities due to
micro-discharges along the ceramic insulators in the electron guns." Will goes
on to note that the problem seems to be more severe in TEMs (which operate at
higher voltages) and these fluids "... have been used successfully for several
years in scanning electron microscopes in some laboratories".

First question:
I'm confused by Will's list-server statement that Krytox is a polyphenylether
when it is listed in his book as a perfluorinated polyether. Not being an
organic (or any other kind) of chemist, I might think that these are synonyms
for the same family of materials except that Will also has a separate
discussion of polyphenyl ether pump fluids (e.g., Convalex and Santovac) which
is separate from that of the perfluorinated polyethers (Krytox and Fomblin). I
think that Krytox actually is a perfluorinated polyether, isn't it?

Second question:
The issue of whether Krytox and Braycote are perfluorinated polyethers is more
than a trivial nomenclature issue because of the alleged problem of
perfluorinated compounds contaminating high-voltage insulators. In addition to
the somewhat equivocal cautionary statements in Will's book, I personally know
of one lab which has banished these compounds from the premises because of an
incident a number of years ago where the glassware used for cleaning of
microscope parts got contaminated with Krytox -- this got transferred to
several TEMs and resulted (so I'm told) in the need to replace multiple guns
over a period of time -- and produced a lot of hair-pulling until the source of
the problem was identified (actually, maybe this should be under the "horror
stories" thread?). But this is the only direct report I have heard of such a
problem and Will, though he notes the concerns, doesn't seem reluctant to
recommend the stuff for EM use (and I do respect his depth of experience in
such things). So my question: Is Krytox really the "bogeyman" I've been told
it is? Or is this just another bit of microscopy folklore?

Given that: (1) there are lots of ways a vacuum grease could get transferred to
a gun indirectly (i.e., via tools or contaminated gloves); (2) this stuff is
reported to be essentially impossible to remove once it gets deposited on
something; (3) the purported HV discharge behavior doesn't show up immediately
but develops gradually over time; and (4) insulator cleanliness is enough of a
problem without introducing this kind of sneaky contaminant -- IF true, it
would seem that this class of compounds has no place in an EM lab. But if this
is all a myth, I'm depriving myself of some otherwise great products. Insight
anyone?

Fred Schamber




From daemon Sat Apr 08 08:18:21 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:22:26 +0100
Subject: Re: sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Most sputter coater manufacturers use one of two methods for mounting their
targets.

1. Target material fixed by a clip or adhesive to an aluminium base
(difficult to sputter without a special coater)

2. Target material fixed by adhesive to a brass base (this will sputter
in relation to its composition e.g. 70%Cu 30%Zn)

You seem to have the type (2) target and are probably sputtering a mixture
of gold (from the outside of the target) and brass (from the middle of the
target). Check it out if you have x-ray analysis?

Sputter targets last a period in direct relationship to target thickness,
coating thickness and degree of use, how long is a piece of string?

Good luck

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com





From daemon Sat Apr 08 08:18:21 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:21:38 +0100
Subject: Re: "S" -distortion in TEM images JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

"S" distortion or anisotropic distortion is produced through a balancing of
aberrations. Take one imaging lens which is being used at low current and
you will have pincushion distortion. Add another lens that is being used in
the low to middle part of its current range and you will have pincushion
distortion. Use the two lenses in a magnification system and the result is
an image with anisotropic distortion.

When we design a TEM we try to balance the lenses so that the degree of
distortion is minimised. Minimised does not mean totally removed it means
NO distortion in the area that falls within the photographic frame. In
early instruments, where the screen was very small, you hardly ever saw the
problem, as screens have increased in size so the problem becomes more
clear.

If the distortion has just started to become annoying this may be due to one
or two reasons.

1) The high voltage has changed such that the lenses are not matched to
the accelerating voltage as they were when the system was new?

2) A lens is not running at the correct level, its current is too low?

The most common reason for a change in lens or high voltage performance are
their reference circuits, I am afraid I am not familiar with these circuits
in the 2000FX.

Good luck hope this helps?

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com





From daemon Sat Apr 08 12:21:05 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 08 Apr 2000 13:08:09 -0500
Subject: Sputter coater cathode

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Joyce Craig wrote:
===================================
How long should a gold target last?
Our target looks gold except in the center.
Problem is that we don't seem to get good coating.
===================================
This is not that uncommon of a question. The first rule of thumb is that if
it does not look like gold, then it probably is not gold. There is one
exception however, and that is when the sputtering process creates some kind
of surface structure that leads to an optical effect, a non-gold looking
gold color (more like grey). But that typically does not adversely effect
the sputtering rate.

The other cases then would be either a) contamination from external sources
(e.g. finger prints) or b) build up of contaminants from the use of gold
with insufficient purity. If (a), then that "problem" is solved by a good
solvent washing and scrubbing with something like acetone. If (b), then it
will not rub off with solvent and it would be something building up in the
way of impurities from within the gold foil itself.

There is a cost associated with taking gold from 0.98 to 0.99 and even more
of a cost to 0.999 purity, then then to 0.9999 or 0.99995 still more cost.
In other words, you really do want high purity gold in the cathodes, for
this very reason, but the higher purities do cost more money. Putting it
another way, a cathode of 0.9999 for example is a lot more expensive than
one that is 0.98 or 0.99, even though the net gold content is about the same
. I won't even begin to speculate on how many would see a difference
between 0.99995 vs. 0.9999 or even 0.999. But there is a point where the
impurity elements that don't sputter begin to build upon the surface,
resulting in a mostly non-gold layer. It is my understanding that the
original equipment manufacturers of sputter coaters and also the main firms
offering replacement cathodes supply only cathodes at the higher end of the
purity scale because of this reason. If you seek non-traditional EM sources
as alternatives, you really have to see the documentation to know that you
are getting high purity, but most of the alternative sources do not deal in
such high purity gold.

If you feel you have followed some of the advice given out on this
listserver about possibly saving money and you could be having a build up of
alloying or contaminating elements, try polishing off this layer in a
metallographic polishing table, in order to renew the original composition
that apparently did work for some time. If you do this, I would be most
appreciative if you could share your experience with the list, be it good or
bad. It might create more of an awareness of the need for high purity gold
when making cathodes.

Disclaimer: SPI Supplies offers replacement gold and other metal cathodes
for sputter coaters used in the SEM laboratory so we would have a vested
interest in customers purchasing their cathodes from SPI or our major
competitors in the EM supplies business.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Sat Apr 08 15:29:41 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Sat, 8 Apr 2000 14:08:28 -0400
Subject: CM12 Ultra High tilt holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does any one have a good condition Gatan 670 Ultra High Tilt Specimen
Holder (80 deg.) for Philips CM12 for sale?


*******************************************************************

Dr. M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Department of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Sat Apr 08 15:29:45 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 08 Apr 2000 14:22:01 -0700
Subject: Re: Perfluorinated Polyether Oils and Greases -- are they a problem ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frederick Schamber wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This is actually a follow-on question to the thread on "Silicone Oils and
} Greases" which ran a few days ago.
}
} In his typically thorough response to the discussion of why microscopists
} generally avoid silicone-based vacuum greases, Will Bigelow noted that
}
} } While the silicone high vacuum grease is
} } indeed a good lubricant for O-rings, it is no better than the Brayco and
} } Krytox greases, which are based on polyphenylether compounds, and which do
} } not introduce the possibility of having insoluble siliceous compounds
} } formed on critical parts of the electron optical column.
} }
}
} This mention of Krytox rang a bell and I checked Chapter 10 of Will's book
} "Vacuum Methods in Electron Microscopy" where he discusses oils and greases.
} In table 10.1 he lists both Braycote and Krytox as "Perfluorinated Polyether
} based" (not polyphenylether). In the subsequent discussion he states that they
} "probably should not be used ... in or near the electron gun because of the
} possibility that some of the perfluorinated base compound might get onto the
} high voltage insulator and cause microdischarges ... ". In chapter 5 he is
} more specific in his discussion of perfluorinated polyether diffusion pump oils
} where he again mentions Krytox and states that use of these pump oils was
} generally abandoned because it was found that "electron microscopes in which
} these fluids were used eventually developed high-voltage instabilities due to
} micro-discharges along the ceramic insulators in the electron guns." Will goes
} on to note that the problem seems to be more severe in TEMs (which operate at
} higher voltages) and these fluids "... have been used successfully for several
} years in scanning electron microscopes in some laboratories".
}
} First question:
} I'm confused by Will's list-server statement that Krytox is a polyphenylether
} when it is listed in his book as a perfluorinated polyether. Not being an
} organic (or any other kind) of chemist, I might think that these are synonyms
} for the same family of materials except that Will also has a separate
} discussion of polyphenyl ether pump fluids (e.g., Convalex and Santovac) which
} is separate from that of the perfluorinated polyethers (Krytox and Fomblin). I
} think that Krytox actually is a perfluorinated polyether, isn't it?
}
} Second question:
} The issue of whether Krytox and Braycote are perfluorinated polyethers is more
} than a trivial nomenclature issue because of the alleged problem of
} perfluorinated compounds contaminating high-voltage insulators. In addition to
} the somewhat equivocal cautionary statements in Will's book, I personally know
} of one lab which has banished these compounds from the premises because of an
} incident a number of years ago where the glassware used for cleaning of
} microscope parts got contaminated with Krytox -- this got transferred to
} several TEMs and resulted (so I'm told) in the need to replace multiple guns
} over a period of time -- and produced a lot of hair-pulling until the source of
} the problem was identified (actually, maybe this should be under the "horror
} stories" thread?). But this is the only direct report I have heard of such a
} problem and Will, though he notes the concerns, doesn't seem reluctant to
} recommend the stuff for EM use (and I do respect his depth of experience in
} such things). So my question: Is Krytox really the "bogeyman" I've been told
} it is? Or is this just another bit of microscopy folklore?
}
} Given that: (1) there are lots of ways a vacuum grease could get transferred to
} a gun indirectly (i.e., via tools or contaminated gloves); (2) this stuff is
} reported to be essentially impossible to remove once it gets deposited on
} something; (3) the purported HV discharge behavior doesn't show up immediately
} but develops gradually over time; and (4) insulator cleanliness is enough of a
} problem without introducing this kind of sneaky contaminant -- IF true, it
} would seem that this class of compounds has no place in an EM lab. But if this
} is all a myth, I'm depriving myself of some otherwise great products. Insight
} anyone?
}
} Fred Schamber


Fred,
I've used Brayco 803 since about 1980 for static seals and have never
had any problems. I used Apiezon L for dynamic seals until I was
introdused to Brayco 602 at which point I noticed cleaner systems once
the Apiezon was gone. Apiezon's vapor pressure numbers look pretty good
until you elevate the temp a little (120F) and then you lose a couple of
decades, if I recall correctly, and the stuff polymerizes like crazy in
an e-beam, but it IS slippery. I've been using the Brayco 602 in gun
translators for a number of years with no problems and much cleaner
guns.

One other thing I've noticed: Old Apiezon oxidizes and turns to a
sticky sludge but the Brayco greases don't seem to age at all.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Sun Apr 09 09:28:04 2000



From: Thomas Litzinger :      thomas_c_litzinger-at-yahoo.com
Date: Sun, 9 Apr 2000 09:09:41 -0500
Subject: Newt retina and lens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am an undergrad from Miami University of Ohio, and
am beginning TEM research with Newt retina and lens.
I have researched various sources to no avail.
Luckily, I was introduced to your network of
Microscopists.
} } My main problem is finding a thorough protocol
for sample preparation that includes such details as
primary fixation, buffers, resin-type, and accurate
times/temperatures. Using the limited knowledge I
have aquired I was able to put together a experimental
protocol, which is polymerizing at this moment. I
would greatly appreciate any advice or leads on proper
protocol in this area.
} }
} }

} Thanks,
} } Thomas Litzinger

__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Sun Apr 09 09:28:04 2000



From: baddis-at-olypen () (by way of Nestor J. Zaluzec)
Date: Sun, 9 Apr 2000 09:12:08 -0500
Subject: Image grabbing with a mieji trinoc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: baddis-at-olypen
Name: barnett addis
Question: I am a retired Phd from another remote disipline in another era
who has become interested im micromount minerals and wish
to do some image grabbing with a mieji trinoc(on order) but have reached
a quandry re cameras. Has anyone had any hands on experience
with a pixeria or a kodak mds100.or other lower priced rigs. i am loking
at these
as affordable options that may provide a better image than the tried and true
method of a color ( below $1,000) video camera and "snappy" as the grabber.
Any comments, rumors, suggestions would be most appreciated

---------------------------------------------------------------------------




From daemon Mon Apr 10 08:15:22 2000



From: richard.beanland-at-gecm.com
Date: Mon, 10 Apr 2000 09:42:54 +0000 (GMT)
Subject: Re: Ungluing M-bond

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ian,
the only really successful way to get rid of epoxy is to use fuming nitric or sulphuric. You can also try aggressive solvents like tetrahydrofuran or dimethylsulfoxide. What will work usually depends upon the composition of your sample - if it also etches in these acids, you may have difficulties. I can get you some recipes from the books on de-encapsulation of silicon chips we have here, if you like.

All these chemicals (particularly fuming nitric) are very unpleasant and need special precautions for use. Swelling of the epoxy is inevitable, and if the specimen is delicate you may need to go through several cycles of nitric/solvent to remove the epoxy without putting too much stress on your sample.

Richard



} Dear all,
} I have made a cross section of something using M-Bond 610 resin and it
} shifted in the clamp while curing so I now have a cross section that would
} be almost impossible to polish to leave the interface vertical. I would
} prefer not to just throw the piece in the bin and start again, due to lack
} of material. Does anyone know a way to dissolve fully cured M-bond 610 so
} that I could start again from scratch?
}
} Thanks
}
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences
} P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
}
} ______________________________________________________
} Get Your Private, Free Email at http://www.hotmail.com

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Mon Apr 10 08:15:22 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Mon, 10 Apr 2000 10:10:02 +0100
Subject: folds in sections - summary (long-ish)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone

I had quite a few suggestions for getting rid of creases and wrinkles
in semi-thin sections - as promised to some of you, here is a summary.
At the end, i have appended some old references which I dug out of my
store over the weekend. i don't know if they all/if any work! I am
still trying. If I get good results, I may post another message!
Meanwhile, on with the folk-lore!

And waiting for the moon to rise!

Keith Ryan
Marine Biological association
Plymouth UK

Folds in semi-thin sections


1. Use lower drying/staining temperatures - e.g. 60 C - when
hotplate is still warming up
2. Dry flat at 50-55 C then go to hotter plate for 10-15 minutes
3. Longer on the hotplate
4. Dry sections on slide using a flame
5. Set hotplate to just below boiling the water
6. Vapour (6 messages)
7. Transfer to 10% acetone droplets
8. Harder resin (2 messages)
9. Softer resin
10. Longer infiltration times
11. Improper mixing
12. Heat pen (3 messages)
13. Chien grids (grid with 2 mm hole and tab for handling) to
transfer thicks to water droplets
14. Wick away the water once section is spread
15. Use big drops, lots of water
16. Cut thinner sections
17. Cleanliness - of all items
18. Stain on drop of stain on hotplate, steam, transfer, water
rinses, dry down
19. Put a nick in the corner of the cutting face so that the resin
can expand differentially to the enclosed specimen
20. Etch the sections with ethoxide (ethanol + NaOH)
21. Remove resin to lessen apparent effect? Using saturated NaOH in
methanol.

22. Coat the slide - One droplet of protein-glycerol is diluted in 1
ml dist. water, then the glass slide is dipped into this solution and
dried in an oven at 30 C. Protein-glycerol: dissolve 1 gram ALBUMIN
(MERCK) in 9 gram bidist. water at 37xC in an oven. Filter the
solution and mix with the same amount of glycerol.


23. W.M. Harris (1978). Stain Technol. 53: 298-300.
Transfer sections to a large drop of 10% acetone. Dry on hotplate
set to 122 C (250 F). It says that! If the sections are known to be
difficult to flatten, add more 10% acetone during the process. Remove
the sections from the hotplate as soon as they are dried down.
EXCESSIVE HEAT AFTER DRYING DOWN CAUSES WRINKLES. Finally, [place
slides on a warming tray at 40-50 C and allow to dry down thoroughly,
30 minutes to overnight. Then stain.

24. M. Martins-Green (1978). Stain Technol. 53: 296-300.
Sections spread with xylene vapour. Floated on toluidine blue stain
(diluted, 1 ml of 1% stain plus 20 mls 2.5% sodium carbonate sol.) at
50 C on hotplate for 1 hour, then left in the dark at room temperature
for 24 hours). Rinsing - not mentioned. Dried on 2-3 drops of
double-distilled water for 10 minutes at 50 C. Cover-slipped. Method
designed to allow collagen fibres to fully distend after cutting.


25. J.R. Sommer et al. (1979) Stain Technol. 54: 106-107
"wrinkles appear during staining rather than while the sections are
being dried on the glass slides - eliminated when the stain is made
up with 50% with respect to glycerol. Staining is not rapid - 12-24
hours in a covered dish at 50 C. Slides need not be albuminized"
Imply staining by flotation at 60 C in a covered dish 12-24 hours,
rinse briefly, dry on slides and view (without a coverslip).


26. J. Millonig (1980) Stain Technol. 55: 118-120.
Paraphrasing: Wrinkles appear when sections are heated during
staining, more so when there is a rim of resin around the tissue.
During heating, the tissue expands more than the tissue. Transfer
sections onto a drop of stain on the end of a slide. Heat in a flame.
Place on a staining bridge (usually 2 minutes). After cooling, float
sections off on water. Rinse in another beaker, transfer to acidified
water. Transfer to slide, warm in a flame, wick away excess water. Do
not need to albuminise the slide. 0.5 micron sections stain in abouit
2 minutes, thinner sections need a second heating and should be left
floating on stain for longer.


27. N.B. Chandler & G.C. Schoenwlf (1983). Stain Technol. 58:
238-240.
State that "wrinkling occurs only when mounted sections are *.
heated".
Sections dried overnight at 76 C on acid cleaned slides, prior to
staining (76 C was the maximum temp. of their hotplate). Acid
cleaning: soak slides for a minimum of 1 hour in 10% potassium
dichromate plus 10% conc. sulphuric acid. Sections dried at 60 C
often wrinkled, while those dried at 90 C often failed to stain
properly. Sections dried less than 6 hours at 76 C often wrinkled
during staining - hence dry overnight. Staining can be controlled
more consistently in Coplin jars placed in a water bath rather than on
a hotplate.


That's all, folks
except for "Hello, Daniele"!




From daemon Mon Apr 10 08:15:23 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Mon, 10 Apr 2000 08:14:03 -0300
Subject: Re: and another horror story

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My horror story is pretty recent: Just last January. We had a local SEM
service guy come in to do a semi-annual tune-up on our ESEM, and he did a
great job. He did notice, though, that our ion pump wasn't giving us quite
as good a vacuum as it should, and was kind of slow doing it. When he left
he said "All you need to do is bake it off a bit with some heat for a few
hours - that'll refresh the active ingredient (or parts) inside and it'll
work better for you. I've got a heater that's designed for just such a
job."
True to his word, a couple days later he dropped off this device for me.
Just a biggish aluminum box in two parts, with luggage clips to lock it
together and a 1500 W heater element inside. Simplicity itself to use, just
turn off your ion pump, clip this box over/around it, and plug it in for a
while. The heater element rests right against the back of the ion pump. So
I mount it on there, plug it in, wait a bit, and sure enough, things start
heating up in there. OK, I think, I'll go see my colleague upstairs for a
few minutes about those samples she was preparing.....
I'm back down in the lab ten minutes later, and go back behind the scope
to see how things are doing. There's a small hole in the back of the heater
apparatus, and when I happen to glance in there, I see a small wall of blue
flame. "This can't be good", thinks I....then "Now which type of fire
extinguisher do you use on a burning ESEM? Water?......No, probably not.
Powder?......uhhhhh.......no. CO2? Probably..." Meanwhile, I unplug the
heater and mostly just stand there....thinking how close I've come to
pensionable retirement age, only to lose it like this.
But anyway, the flame started to die back a bit as soon as I unplugged the
thing, and with some damp towels I was soon able to unclip it and remove
it. It turns out our particular instrument had a clip mounted on the back
of the ion pump to retain the HT cable in place, and this clip had been
mounted on piece of black plastic, which I swear to God I thought was
anodized aluminum. The plastic had melted with all that intense heat of
course, and dripped right down onto the heater element, where it had burst
into flame.
I had our machine shop make me a metal replacement for the lost plastic
bit, there was no other damage, by God, the ion pump now works great, and I
figure local management doesn't need to hear about every little detail of
life in the SEM lab, now, do they?


Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Mon Apr 10 08:15:24 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Mon, 10 Apr 2000 12:34:49 +0200
Subject: Sludge analysis

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G'day Cobbers!
I could use some handy hints here. We've a student who wishes to
analyse some hideous sludge - something to do with sewage me thinks. She
is particularly interested in the state of the metals in this muck -
whether it is present as sulphides, sulphates, nitrides, nitrates or
bound to organic molecules. The stuff is rather fine grained, and I've
suggested she filter and retain the } 10 micron fraction. She will then
press it into a pellet with a flat, shiney surface if all goes well.
Basically, I'm interested if there is anyone out there who has
experience of analysing this type of material. What did you coat it
with? How did you differentiate between the the various form of the
metal compounds? Any other handy hints also gratefully received.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Mon Apr 10 08:15:25 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Mon, 10 Apr 2000 08:12:01 -0700
Subject: Re: Ungluing M-bond

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Hi Ian,

I'm not sure what the Measurements Group uses to cross-link the M-Bond gage cement. The resin is listed as an "epoxy-phenolic adhesive". In several anhydride cross linked systems I've found that sodium ethoxide in ethanol worked well to de-resin samples. I made mine fresh from sodium and ethanol but fresh anhydrous AR grade should work as well. Something like 5 or 10% in dry ethanol should do it. This may be
easier on your samples if they are acid sensitive. I'd be curious what works as I can see myself in a similar predicament someday.
cheers,
John

John Heckman
MSM Department
Michigan State University

richard.beanland-at-gecm.com"-at-sparc5.microscopy.com wrote:

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} Hi Ian,
} the only really successful way to get rid of epoxy is to use fuming nitric or sulphuric. You can also try aggressive solvents like tetrahydrofuran or dimethylsulfoxide. What will work usually depends upon the composition of your sample - if it also etches in these acids, you may have difficulties. I can get you some recipes from the books on de-encapsulation of silicon chips we have here, if you like.
}
} All these chemicals (particularly fuming nitric) are very unpleasant and need special precautions for use. Swelling of the epoxy is inevitable, and if the specimen is delicate you may need to go through several cycles of nitric/solvent to remove the epoxy without putting too much stress on your sample.
}
} Richard
}
} } Dear all,
} } I have made a cross section of something using M-Bond 610 resin and it
} } shifted in the clamp while curing so I now have a cross section that would
} } be almost impossible to polish to leave the interface vertical. I would
} } prefer not to just throw the piece in the bin and start again, due to lack
} } of material. Does anyone know a way to dissolve fully cured M-bond 610 so
} } that I could start again from scratch?
} }
} } Thanks
} }
} } Ian MacLaren
} } Beijing Laboratory of Electron Microscopy
} } Chinese Academy of Sciences
} } P.O. Box 2724
} } 100080 Beijing
} } China
} } General Email: ian.maclaren-at-physics.org
} } Work (esp. large attachments): maclaren-at-image.blem.ac.cn
} }
} } ______________________________________________________
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} ==============================================================
} Richard Beanland
} Caswell Technology,
} Caswell,
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}
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} Tel. +44 1327 356363
} Fax. +44 1327 356398
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} Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."



From daemon Mon Apr 10 08:15:27 2000



From: anderron-at-us.ibm.com
Date: Mon, 10 Apr 2000 08:51:26 -0400
Subject: Sludge analysis

Contents Retrieved from Microscopy Listserver Archives
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A microprobe wouldn't be my first choice for analyzing the sludge.
Essentially, you'd wind up with a list of elements. Old fashioned x-ray
diffractometry would yield a list of PHASES, which would be a much greater
help. A microprobe's list of elements would, of course, be a great aid in
the x-ray diffraction search/match operation. Check out the International
Centre for Diffraction Data's web site for the latest computer
search/matching things. www.icdd.com (or is it .org?)

Ron

Cobber?

(I have no financial interest in the ICDD)


Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



Dr Malcolm Roberts {malc-at-rock.ru.ac.za} on 04/10/2000 06:34:49 AM

To: Microprobe discussion group {Microscopy-at-sparc5.microscopy.com}
cc:


G'day Cobbers!
I could use some handy hints here. We've a student who wishes to
analyse some hideous sludge - something to do with sewage me thinks. She
is particularly interested in the state of the metals in this muck -
whether it is present as sulphides, sulphates, nitrides, nitrates or
bound to organic molecules. The stuff is rather fine grained, and I've
suggested she filter and retain the } 10 micron fraction. She will then
press it into a pellet with a flat, shiney surface if all goes well.
Basically, I'm interested if there is anyone out there who has
experience of analysing this type of material. What did you coat it
with? How did you differentiate between the the various form of the
metal compounds? Any other handy hints also gratefully received.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA








From daemon Mon Apr 10 17:51:24 2000



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Mon, 10 Apr 2000 10:31:10 -0500
Subject: Critical Point Drying

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We are attempting to critical point dry 100 micron thick Vibrotome
cut cross sections of annelids (leeches). The end product looks fine
in the SEM, except that the sections have curled/rolled up during the
process, and we want them to remain flat. Attempts to flatten them
after drying have not been very successful. Has anyone devised some
sort of holder (sandwich?) that might overcome this problem by
keeping them flat during their trip through the dryer?

We thank you in advance.

Dick Briggs
Biology Department
Smith College



From daemon Mon Apr 10 17:51:25 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 10 Apr 2000 11:49:23 -0600 (MDT)
Subject: Wrinkles in semi-thins

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This worked for a student a few months back working on nanoporous zirconia.

Ion mill the sample from one side long enough to remove the polishing damage
from that side. Remove from the ion mill and coat the milled side with
carbon. Return to the Ion mill and mill on the uncoated side. The carbon
on the other side will help keep the thinned grains from falling out.

I got this trick from Scott Walck some time ago.

Ray




*************************
Ray D. Twesten, PhD
Center for Microanalysis of Materials
University of Illinois
(217) 244-6177 fax:(217) 244-2278

----- Original Message -----
} From: "Valerie Leppert" {vjleppert-at-ucdavis.edu}
To: "'Microscopy Listserver'" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, April 07, 2000 4:55 PM



Hi,

If you give me a worm and tell me to produce semi-thin sections that
wrinkle, and semi-thin sections that do not wrinkle, I can do it. But I
might need two worms!

Stretching, hot plate and water manipulations, cutting contortions,
cleanliness, etc., are the band-aids. These methods do not address the
basic problems of which there are at least two.

At the very base of the problem usually lies the fact that there is too
high of a percentage of unbound monomers in the tissue. This can happen
in at least several ways 1) The use of a good formulation which is not
suitable for the tissue 2) the use of a formulation which has no basis in
correct composition as far as molecular weights and WPE #s are concerned.
These formulations are usually handed down from lab to lab and no one
knows their origins. 3) inadequate infiltration procedures of a good and
suitable formulation. All of the above will leave too many unbound
monomers in the tissue.

So what? Unbound monomers are extremely active. They attract water,
swell, pull, stretch the embedded tissue. Upon drying the water leaves
since it is mechanically bound, and presto, nice wrinkles.

Let us look at 1) above. A formulation containing Araldite, and a hefty
amount of DDSA is unsuitable for tough skin, collagen, worm architecture,
and so on. Why? Because Araldite and DDSA are very long chains, and are
extremely difficult to keep EVENLY mixed and EVENLY infiltrated into
tough tissue. Note the capitalization of evenly! Some of the Araldite
will stay outside of the worm gut, and there will be more there than
inside the gut. Inside the gut there is an excess of DDSA. Crosslinkage
and stability are reduced. Water will be absorbed in the boat by these
loose monomers.

I cannot deal with 2) at all. I never use any formulation unless I
understand it. No telling what the proportions are!

Let us look at 3). Inadequate infiltration procedures account for more
trouble than than Monica Lewinsky! Formulations seperate, are allowed to
infiltrate unevenly, or not thoroughly enough, or not slowly enough.
Perhaps the dehydration was not adequate, and the formulation will not
take up a space occupied by a polar substance. Again, you have loose
monomers which should be bound or crosslinked.

What would I do with my worms? For wrinkles I would use
Araldite-DDSA-Epon, infiltrate it poorly, underpolymerize it, and watch
the pleats appear. For flat sections I would pick a formulation which
would fit the material - perhaps the next to the hardest Luft's
formulation, infiltrate it like crazy, never let it sit in the hood, heat
every new change of new mixture to 37 degrees for one hour and a half with
a light bulb while they tissue rotates, do many changes over perhaps 48
hours, at least. I would have really dehydrated well, gone to Propylene
oxide, the infiltrate starting with a mixture of 3 parts propylene oxide
and one part formulation, gradually working up to full epoxy mixture.

I would polymerize for 48 hours at least and cut the thinnest section I
could tolerate for my study. I would use a sharp knife (of course).

Now, it might not work well the first time. So I would manipulate all the
above until I was happy.

NOW. PLEASE NOTE: Suppose I have sections which are a little wrinkled,
or some old blocks which yield severely wrinkled sections. I soak the
slides in rt water for half an hour or less depending on how well they
will stick to the slide. Then I would put the slides into a vaccum jar
which has that strong, powdered dessicant which we used to use for drying
EM film in a petri dish in the bottom. (Protect the slides from flying
poweder by covering with hardened filter paper). Then evacuate the jar
just short of implosion! Release the vaccum in about 24-48 hours. This
will often save poor blocks or totally get rid of slight wrinkles.


Crowley, Hildegard Heinrich, and Ben H. Leichtling. Elimination or
Reduction of Wrinkles in Semithin Epoxy Sections by Vaccum Drying. Stain
Technology,Vol 64, No 5, September 1989.

Please do not ask me for reprints. I do not have any.

Have fun with the worms! Keep records! Know what you have done, and what
you have not done!

Hildy

P.S. Very important! Formulations made from epon substitutes which
contain Araldite or dilutants may not be suitable for use with difficult
materials, since they may be too resilient or elastic. We have used
Eponate 12 from Pella forever. Many years ago I had information regarding
the spec readings of this substitute - it was very much like the old Epon.
I am hazy on this point. Do not quote me. I do not have any monetary
interests in Pella.



From daemon Mon Apr 10 17:51:27 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 10 Apr 2000 15:56:19 -0400
Subject: Re: Sludge analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear "Malc",

I'd suggest you also do so good old fashioned light microscopy on a "sludge
smear". We've had the opportunity to use McCrone's Particle Atlas on CD
ROM on several courses recently and have found it invaluable in identifying
things like this. McCrone was running a special at PITTCON: $700 instead
of the usual $900. The last time the PA came out in print, it was
approximately 10 volumes in length. The CD has light, electron, and
elemental analysis info. I would be surprised if there isn't a copy of
either the print or CD version in your library.

Caveat: MME has no financial interest in this product.

Good hunting!
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^




At 12:34 PM 4/10/00 +0200, Dr Malcolm Roberts wrote:
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From daemon Mon Apr 10 17:51:28 2000



From: Karen Rethoret :      rethoret-at-yorku.ca
Date: Mon, 10 Apr 2000 16:05:21 -0400 (EDT)
Subject: Toronto Area Position wanted

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I am writing this on behalf of an experienced SEM Technician, new to
Canada who is looking for a part or full time position, (will even work as
a volunteer for now) in the Greater Toronto Area.

She has 20 years of experience working on various materials on JEOL SEM's
in Hungary but needs help in establishing contacts and learning the
industry here.

Please contact me off-line if you are interested.

Karen Rethoret
York University Microscopy Facility
Toronto, Ontario
416-736-2100 x33289



From daemon Mon Apr 10 17:51:29 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 10 Apr 2000 17:18:14 -0400
Subject: Re: Sludge analysis

Contents Retrieved from Microscopy Listserver Archives
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Dr Malcolm Roberts wrote:

Dear Malcolm,

} I could use some handy hints here. We've a student who wishes to
} analyse some hideous sludge - something to do with sewage me thinks. She
} is particularly interested in the state of the metals in this muck -
} whether it is present as sulphides, sulphates, nitrides, nitrates or
} bound to organic molecules.

I reported on using EDS to localize PCBs in sediment at the
Microscopy and Microanalysis meeting in 1998 (page 498 in the
proceedings). Fortunately for me, there is no Cl in sediments, so
just locating Cl was indicative of PCBs. Your student could get
chemical info from microanalysis with very high energy resolution
by looking at the fine structure. Either EELS (on an instrument
with a FEG) or WDS could have this resolution, and if the new
technologies of superconducting tunnel junction diodes or micro-
calorimeter detectors are available, they could provide sufficient
resolution for EDS. Taking position-tagged spectra on, e.g., a
Zeiss 912 could give her both the localization and chemical info
she needs. I have no interest in either Zeiss or the new types of
detector except as a would-be user and techno-geek.


} The stuff is rather fine grained, and I've
} suggested she filter and retain the } 10 micron fraction. She will
} then press it into a pellet with a flat, shiney surface if all goes well.

I just suspended the sediment, put a small amount on a grid,
and took images and spectra. I used all but the mm-sized particles;
most were in the micron to submicron size range. They were well-
dispersed on the grid when the dilution of the suspension was ap-
propriate, and I could get spectra from individual particles. This
approach may be better than the pellet, since different particles
can have different chemistries.


} Basically, I'm interested if there is anyone out there who has
} experience of analysing this type of material. What did you coat it
} with?

I didn't coat it; the high-voltage microscope is capable of
getting good images from this material as is (no charging). The
IVEM, likewise, was able to get good spectra.


} How did you differentiate between the the various form of the
} metal compounds? Any other handy hints also gratefully received.

As I said, I didn't need to get chemical info, and as another
poster said, using diffraction to characterize the material can add
info about the form the metal is in. Reflection high-energy elec-
tron diffraction (RHEED), low-energy electron diffraction (LEED),
and photoelectron holography (PEH) can give info about surface
structures (see, e.g., Leslie et al. (1999) Electron Crystallography in
Surface Structure Analysis, Microscopy Research and Technique
46:160-177). If the metals are adsorbed to the sludge by adhering
to a facet with a particular crystallographic orientation, either as
occasional adatoms or, better still, as an epitaxial deposit, such
surface techniques could be useful. Perhaps Larry Marks is the
best person to talk to about this. Good luck.
Yours,
Bill Tivol




From daemon Mon Apr 10 17:51:29 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 10 Apr 2000 16:34:13 -0400
Subject: I GOOFED on Brayco & Krytox

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My profound apologies to everyone who read my comments on silicone oils and
greases, in which I said that Brayco and Krytox greases are based on
polyphenyl ether compounds. I was in too much of a hurry, and neglected to
check my memory (which isn't getting any better as my maturity progresses).


Fred Schamber is right in calling attention to the fact that these greases
are based on perfluorinated polyether compounds.

Thus, the information given on page 460 of my book 'Vacuum Methods in
Electron Microscopy' is correct: the Brayco and Krytox greases are based
on perfluorinated polyether compounds, NOT polyphenyl ether compounds.

Furthermore, Fred is correct in stating that great care should be exercised
to keep the perfluoro-polyether compounds out of the electron guns of
electron microscopes, because, as discussed on p. 187 of Vac. Meth. in EM,
they have been known to cause microdischarges in electron guns causing
undesirable high-voltage instabilities.

Although I have not bothered to try to keep up with all new developments
since I finished writing my book, back in '94, I am not aware of any vacuum
grease that is based on polyphenylether fluids. This is peculiar, because
the polyphenyl ether diffusion pump fluids, Santovac-5, Excello-54, and
Convalex-10, have all worked out so well. A grease is made by combining an
oil with a gel-forming agent. Thus, to make an ordinary lubricating grease
one might mix a soap such as sodium stearate with an ordinary lubricating
oil. When heated to a proper temperature the oil and soap will interact to
form a gel, which we call a grease. Thus, to make a grease based on the
polyphenyl ether oils, all one would have to do is to find the appropriate
gelling agent. This is not an entirely trivial task, however, because
unless the correct agent is found the grease will 'bleed' (i.e. the oil
will separate from the gelling agent), especially if the grease is heated
or exposed to a vacuum. If a grease bleeds, you can end up with only the
gelling agent remaining on the bearing or gasket that you wanted to
lubricate, while the oil (which is the actual lubricating agent) spreads
around and contaminates adjacent parts. The silicone- and perfluorinated
polyether-based greases are remarkably stable and resistant to bleeding,
whereas some other high vacuum greases are not.

Again, I apologize for my mistake.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Mon Apr 10 17:51:32 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 10 Apr 2000 17:19:23 -0500
Subject: Re: sputter coater target

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I have also had an interesting, and aggravating problem with sputter coater targets recently. We have an older model Hexland (bought out by Oxford about 10 years ago) SEM cryo stage. Although not as fancy as current models, it usually does a reasonable job. Quite a number of years ago I purchased a large gold disc sputter coater target. I cut the 12mm discs needed for the sputter coater in the cryo prechamber from this larger disk with a simple stamping tool. This worked for years without problems.
About a year or so ago, we started having problems getting good coatings...noticable because of excessive charging of coated samples. We immediately thought of contamination because the target was becoming discolored after only a couple of uses. If I removed it, cleaned it with metal polish, etc and reinstalled it, all was well for another couple of coatings. We tried new pumps, looking for oil leaks etc. but a good vacuum in the chamber ruled out large leaks and all other signs were negative. We also changed the argon tank thinking that perhaps this could be a source of contamination.
The cryo stage is used in spurts and after a period of inactivity, we are again gearing up for heavier use. Recently the target was only giving a good coating on one sample before having to be cleaned....a major job when you have to warm to room temp from -170oC prior to opening up the chamber. I finally took the used target over to a Microprobe on campus and did a WDS analysis on it....turns out that the contamination was aluminum. The housing that holds the target in place is aluminum. Somehow the aluminum is being sputtered as well as the gold, producing a poor metal coating and contaminating the target as well.
Oxford is trying to get replacement parts for the sputter head but we are at a loss to figure out why, after all these years, this is happening and what we can do about it if replacement parts cannot be found.
By the way, a new crystage runs about $70,000 so we are not contemplating that move at the moment.
Debby


Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057
On Saturday, April 8, 2000, Garber, Charles A. {cgarber-at-2spi.com} wrote:
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From daemon Mon Apr 10 18:01:36 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 10 Apr 2000 18:55:45 -0500
Subject: M-Bond 610

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ian MacLaren wrote:
=======================================================
} Dear all,
} I have made a cross section of something using M-Bond 610 resin and it
} shifted in the clamp while curing so I now have a cross section that would

} be almost impossible to polish to leave the interface vertical. I would
} prefer not to just throw the piece in the bin and start again, due to lack

} of material. Does anyone know a way to dissolve fully cured M-bond 610 so

} that I could start again from scratch?
}
} Thanks
}
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences
} P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
=============================================================
We have found over the years that the easiest way to remove not just M-Bond
610 but just about any epoxy or other organic polymer from a metal substrate
is with the use of oxygen plasma etching. It was my recollection that one
of our systems was purchased by the KYKY part of your facility and they
would probably let you have access to it.

Disclaimer: SPI Supplies manufactures the Plasma Prep II plasma etcher
which can be used to remove organics, including intractable cross-linked
polymer systems, like M-Bond 610.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Mon Apr 10 18:11:35 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 11 Apr 2000 11:00:47 GMT+1200
Subject: Cobber

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}
} Ron
}
} Cobber?
}

Cobber = Bro = Mate = Buddy

rtch (another cobber from the Southern half of the world)

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Apr 11 17:10:45 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Monday, April 10, 2000 5:26 PM
Subject: Re: sputter coater target

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Dear Debby:

Here is my guess about what has happened. I read someplace that alumina
(aluminum oxide) does not sputter easily whereas pure uncoated aluminum
metal sputter easily. Aluminum in air is quickly coated with alumina and it
is protected for a while when placed in an area subject to ion bombardment
for sputtering. The alumina layer on your target holder has been worn away
by years of sputtering and cleaning, and in the cold vacuum of the cryo
prechamber was not getting replaced by oxidation.

Cure: Take the holder out and have it anodized (a hard finish) or just heat
it gently in air for a while to form a new layer of aluminum oxide.

Ronald Vane
XEI Scientific


-----Original Message-----
} From: Debby Sherman {sherman-at-btny.purdue.edu}
To: message to: MSA list {microscopy-at-sparc5.microscopy.com}



From daemon Tue Apr 11 17:10:52 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Tue, 11 Apr 2000 01:17:20 -0500
Subject: Re: sputter coater target

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Debby,

Could the alumium have had some kind of coating to keep it from sputtering
or could a short developed between the gold disk and the alumium
housing? My guess is that there is a break down in the insulation between
the target and the holder. If you have records of the current and voltage
it sould show up when compaired with the old records when things were
running right.

Could some other part of the system be contaminated by Al? If the
rest of the system is not made of alumium you can use lye to clean
it.

When a new machine costs $70 K you can afford to pay a good machinest
for a lot of hours to make a part. With CAM mills they can make almost
anything. And for what the computer controled mill can't make there are
a few of us old guys that can finish the job with a file:) You porbably have
a couple in one of the insterment shops on campus.

If you don't have a shop that can handle this kind of work I know a couple
of guys out on the west coast that can. I don't have any connection with
them except to admire their work.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00


}
} I have also had an interesting, and aggravating problem with sputter
coater targets recently. We have an older model Hexland (bought out by
Oxford about 10 years ago) SEM cryo stage. Although not as fancy as current
models, it usually does a reasonable job. Quite a number of years ago I
purchased a large gold disc sputter coater target. I cut the 12mm discs
needed for the sputter coater in the cryo prechamber from this larger disk
with a simple stamping tool. This worked for years without problems.
} About a year or so ago, we started having problems getting good
coatings...noticable because of excessive charging of coated samples. We
immediately thought of contamination because the target was becoming
discolored after only a couple of uses. If I removed it, cleaned it with
metal polish, etc and reinstalled it, all was well for another couple of
coatings. We tried new pumps, looking for oil leaks etc. but a good vacuum
in the chamber ruled out large leaks and all other signs were negative. We
also changed the argon tank thinking that perhaps this could be a source of
contamination.
} The cryo stage is used in spurts and after a period of inactivity, we
are again gearing up for heavier use. Recently the target was only giving a
good coating on one sample before having to be cleaned....a major job when
you have to warm to room temp from -170oC prior to opening up the chamber. I
finally took the used target over to a Microprobe on campus and did a WDS
analysis on it....turns out that the contamination was aluminum. The housing
that holds the target in place is aluminum. Somehow the aluminum is being
sputtered as well as the gold, producing a poor metal coating and
contaminating the target as well.
} Oxford is trying to get replacement parts for the sputter head but we
are at a loss to figure out why, after all these years, this is happening
and what we can do about it if replacement parts cannot be found.
} By the way, a new crystage runs about $70,000 so we are not
contemplating that move at the moment.
} Debby





From daemon Tue Apr 11 17:10:55 2000



From: gao-at-nirim.go.jp (Gao Yihua)
Date: Tue, 11 Apr 2000 16:37:50 +0900 (JST)
Subject: Who can introduce some classical books

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Dear Colleagues:

Who can introduce some classical books and references about the

interfacial structure between different materials?


Thanks in advance.

Yours sincerely
Gao Yihua




From daemon Tue Apr 11 17:10:55 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Tue, 11 Apr 2000 08:58:05 +0100
Subject: Re: Wrinkles in semi-thins

Contents Retrieved from Microscopy Listserver Archives
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Hildy

Thanks for all the info.

The resin used was a TAAB Laboratories standard Hard pre-mix kit,
mixed and kept in a freezer. Usually no problem but the resin mixture
may have aged.

Infiltration may not have been sufficient, although it was overnight
in the 50/50 mixture acetone/resin (our safety dept. is anti propylene
oxide). We can usually get away with this procedure, but you may have
some good points about this step. I am suddenly back about 20 years!
Our library stopped taking Stain Technol. in the 1980's and I haven't
searched it since!

The wrinkles seem to be most apparent in the area of the cuticle
(which is really not very thick).

So long

Keith Ryan



From daemon Tue Apr 11 17:10:55 2000



From: Van Osta, Peter [JanBe] :      PVOSTA-at-janbe.jnj.com
Date: Tue, 11 Apr 2000 09:58:37 +0200
Subject: Digital imaging concepts in microscopy

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Hi,

I hesitated a bit to post this message as I thought it would be too trivial,
but my personal experience has shown me that people often do not know the
basic concepts needed to acquire good quality images with a digital/video
camera in light microscopy. I want to know if other people working in
digital imaging agree on the very few concepts I think that are essential to
acquire good quality digital images in microscopy.

I simply give an overview of the concepts for quantitative digital
brightfield microscopy:

1) Understand the meaning of Numerical Aperture
2) Use white light, dim with neutral density filters if necessary, do not
turn down the light to "reddish".
3) Set up Koehler illumination !
4) Nyquist sampling (match magnification to CCD-array)
5) Understand the influence of the sampling density on the C.V. of your
measurements
Ian T. Young, Sampling density and quantitative microsocopy Analytical and
Quantitative Cytology and Histology, vol. 10, 1988, pp. 269-275

6) Understand the dynamic range of your image acquisition system (camera +
digitiser)

For a B/W camera:
1) Use a green filter for monochromatic light

For a color camera
1) Use a 3CCD camera, not a single CCD camera
2) Set the white balance

My personal opinion is that if you obey these basic rules you get good
quality images, otherwise you don't. The quality of the images relates
directly to the quality of your analysis and as such to the "quality" of
your conclusions.

Regards,

Peter Van Osta, MD

Senior Scientist Medical Image Analysis
Biological Imaging Laboratory
Life Sciences Department I - 6065
Janssen Research Foundation
Turnhoutseweg 30
B-2340 Beerse
Belgium
Europe

tel.: +32 (0)14 60.60.65 (office hours, GMT+1)
fax.: +32 (0)14 60.57.88

email: pvosta-at-janbe.jnj.com

WWW: http://ourworld.compuserve.com/homepages/pvosta




From daemon Tue Apr 11 17:11:03 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 11 Apr 2000 11:37:59 +0100
Subject: Tissue Tek machine evaluation

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Dear Collegues

I am in the process of acquiring a Tissue Tek automatic tissue processor,
second hand.
I seem to have a choice between Tissue Tek II or Tissue Tek III, the later
being more expensive of course.
Since I have no experience with such machines, I would like to ask if in
practice there are significant benifits from the more advanced model.
Actually what I want is a reliable way of making lots of paraffin embeddings
with a minimum of sofistication.


Thanks in advance for your answers

Dr. A.P. Alves de Matos
Dental Medical School
Lisbon




From daemon Tue Apr 11 17:11:05 2000



From: Leslie Eibest :      leibest-at-duke.edu
Date: Tue, 11 Apr 2000 08:30:37 -0400
Subject: one more horror story

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A grad student that I had recently trained to use our JEOL
T-20 SEM was trying a little solo work. He had the specimen chamber
door open, turned away from the scope, and looked back just in time
to see one of our monster cockroaches disappearing into the scope.
Rather than call me, or wait for the roach to reappear, he shut the
door and pumped the scope down. He never did confess...



From daemon Tue Apr 11 17:11:09 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Tue, 11 Apr 2000 16:09:19 +0200 (METDST)
Subject: EELS: need spectrum for comparing

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Thanks Norm for your suggestions

I will be more specific.
I will need to process up to 60 specimens/week in two or three separated
weeks during the year. The rest of the time hand processing is OK. Since the
specimens are prone to dammage upon storage (immunohistochemical techniques
on sponge tissues), I do not want to store them to facilitate processing.
So, it is important to know if the machine can handle this amount of work.
I have one technician that has many other things to do.
Basic assistence should be available from our technical department. Anyhow I
would not trust newer instruments just because they are more recent. I know
of (and have) a few super-new machines (amaizingly expensive) that had to be
thrown to garbage because no one (assistence included) was able to put them
to regular work. It is important to know if the machine model is a well
tested one that usually works for years with no problems or if it has
regular problems.
Since histology and histopathology labs of my knowledge use other tissue
processors, I was not able to get specific advice locally about these
machines.

Thanks
A.P. Alves de Matos

----- Original Message -----
} From: Norm Granholm {granhona-at-email.uc.edu}
To: A.P. Alves de Matos {apmatos-at-ip.pt}
Sent: Tuesday, April 11, 2000 12:57 PM


Hello,

I`m looking for an EELS spectrum containing Oxygen K-edge and some
other edge like Mn or Ti L-edge. Together with low loss spectrum and data
about the angles.
The spectra need to have a power of 2 (1024 for inst.) bins and low loss
and high loss should have same size.
I want to use this for comparison, i`m trying to do some quantitative EELS
work but so far with limited success.
I want to find out wether my program or my data is wrong;)

Thanks,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************



From daemon Tue Apr 11 17:11:15 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 11 Apr 2000 09:39:01 -0500
Subject: Re: critical point drying

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Try sandwiching the sections between pieces of filter paper. We do this with lots of small samples which we are in danger of loosing. We use large washers as holders for the filter paper sandwiches. The pairs of washers can be secured using small binder clamps (an office product used like paper clips). Or you can knotch the washers so that you can use a piece of wire to hold them together. The knotches keep the wire from slipping off. If you cannot find washers with the correct internal and external dimensions, they are easily made in any machine shop.
I would put the sandwiches together before or during dehydration and then carry the sections through the last ETOH changes as a unit. It takes a bit longer to CPD due to the absorption of the ETOH by the paper but you have a good chance of having flat sections at completion.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

On Monday, April 10, 2000, Dick Briggs {rbriggs-at-Science.Smith.edu} wrote:
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From daemon Tue Apr 11 17:11:17 2000



From: Phoebe J Doss :      pjdoss-at-okstate.edu
Date: Tue, 11 Apr 2000 10:04:57 -0500
Subject: vibration due to truck traffic?

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Microscopist:

Our new lab is supposed to be built next to a four lane highway that has a
lot of 18 wheeler truck traffic. I am concerned about vibration problems
and would like to convince administration that the building should be built
on the other side of the property as far away from the highway as possible.
Any suggestions or known references covering this? Thanks in advance.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Oklahoma State University



From daemon Tue Apr 11 17:11:17 2000



From: ComCryLab1-at-aol.com
Date: Tue, 11 Apr 2000 11:11:04 EDT
Subject: LM-microscope donations needed

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Looking for a few used B & L 7 to 30 power optical
microscopes (or similar- A. O., etc.) to be given to
local vo-tech school. I will pay shipping.
Will trade something if required.
Mike Urbanik
www.crystalguru.com



From daemon Tue Apr 11 17:11:20 2000



From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 11 Apr 2000 12:25:38 -0400
Subject: Re: vibration due to truck traffic?

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http://www.biotech.ufl.edu/icbr/emcl/db/good_vibrations.html

This is a discussion archived on "Tips & Tricks" from a couple of years ago
dealing with a similar problem. E-mail addresses of the poster and
respondents are included so you might discuss things with them further.

Good luck


At 10:04 AM 4/11/2000 -0500, you wrote:
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} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
EM Technician Gainesville, FL 32610
University Of Florida ph 352-392-1184
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "







From daemon Tue Apr 11 17:11:21 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Tue, 11 Apr 2000 12:40:40 -0400
Subject: Re: vibration due to truck traffic?

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Phoebe:

They want to put your lab next to a 4-lane highway...this is a joke, right?

Larry ;-)

PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
another suggestion: make some vibration measurements and check it out...





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Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Tue Apr 11 17:11:22 2000



From: Nathan Haese :      nathan_haese-at-CompuServe.COM
Date: Tue, 11 Apr 2000 12:50:07 -0400
Subject: vibration due to truck traffic? - Dow Chemical Analytical Lab

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Phoebe,

Several years ago, Dow built a state-of-the-art analytical chemistry
building (including a microscopy lab) in Midland, Michigan. One of the
issues they addressed was nearby truck traffic, just like you have. They
ended up closing the road. Bob Czeislinski (sp?), a member of this
listservice, might be able to help you on some of the tech issues raised to
get the change. (Sorry, I do not have his email address.)

Good luck,

Nathan Haese
Lafayette, CA



From daemon Tue Apr 11 17:11:22 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 11 Apr 2000 10:47:18 -0600 (MDT)
Subject: Re: Wrinkles in semi-thins

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On Tue, 11 Apr 2000, Keith Ryan wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hildy
}
} Thanks for all the info.
}
} The resin used was a TAAB Laboratories standard Hard pre-mix kit,
} mixed and kept in a freezer. Usually no problem but the resin mixture
} may have aged.
}
} Infiltration may not have been sufficient, although it was overnight
} in the 50/50 mixture acetone/resin (our safety dept. is anti propylene
} oxide). We can usually get away with this procedure, but you may have
} some good points about this step. I am suddenly back about 20 years!
} Our library stopped taking Stain Technol. in the 1980's and I haven't
} searched it since!
}
} The wrinkles seem to be most apparent in the area of the cuticle
} (which is really not very thick).
}
} So long
}
} Keith Ryan
}
}
Hi,

One should never leave tissue of any sort in a solution of solvent and
epoxy overnight. It is deleterious to tissue structures and does not
serve to infiltrate.

I do not have a worm. But if I suddenly had one to embed and I had no
experience with worms, I would be very suspicious of it. I would
immediately treat it as though it were really hard to handle. I would do
all the dehydration steps for at least 30 min with a change of ethanol
every 10 minutes. I would open a new bottle of 100% ethanol for the last
three changes. I would use propylene oxide, and evaporate the waste from
a bucket in the hood. I would use PO (dry acetone if you must) and epoxy
in the ratios of 3 to 1 for 1/.2 hour, 2 to one for one hour, 1 to 1 for 2
hours. Then one hour in fresh expoxy. Then new, clean vials, with fresh
epoxy. Perhaps 3 times for 2-3 hours each. Every time a new mixture is
added I would heat with a light bulb directed at the rotator to 37 deg
(not over 40) for about 1.5 hours. Then I would leave it overnight on the
rotator, and start again in the morning. A pain in the neck! But then,
science frequently sucks! In the evening I would embed in fresh material,
and immediately polymerize at 60 deg. NOTE: All steps on rotator! Do
not let material stand in the hood, not even for an hour.

I know nothing of your epoxy kit. I cannot comment on it. Since you have
experience with it, I would not immediately throw it out. Just try
pushing infiltration hard, and then try the vaccum trick. It saved a
whole project for me once.

Good luck,
Hildy



From daemon Tue Apr 11 17:11:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 11 Apr 2000 11:01:22 -0600 (MDT)
Subject: Re: Stretch?? Epoxy sections?

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On Thu, 6 Apr 2000, Rosemary White wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Hildegard,
}
} To follow up a posting a few days ago, what epoxy are you using that does
} not compress during sectioning? I am very curious!
}
} THanks,
}
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} phone 61-2-6246 5475
} fax 61-2-6246 5000
} email r.white-at-pi.csiro.au
}
}
}
}
There is no formulation that can be said to not compress during
sectioning. It depends on the way it is handled and the nature of the
tissue being embedded. Please read my postings again which explain in
detail what the basic problems are.

Bye,
Hildy



From daemon Tue Apr 11 17:11:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 11 Apr 2000 10:58:40 -0600 (MDT)
Subject: Epoxy formulations

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Hi,

Someone asked me what I use in the laboratory for epoxies, and now I
cannot find the person who asked.

Our general standard is the Luft' medium formulation. It is a requirement
however for reembedding Vibratome or thick sections from glass slides, no
matter what the original section was embedded in. We use Pella Eponate 12
and all other resins come from EMS. (I have no financial holdings in these
companies)

If the end of the project goes only to thick sections, we use
Mollenhauer's Araldite - Epon - DDSA - dibutyl, because this mixture
sections like butter. However, we have to embed keratinized skin so the
embedding procedure is really lengthy.

For immunocytochemistry we try to find out if we have huge quantities of
antigen. We first try one embedment (this is for post-gold) with the
above Araldite 502 mixture. If at first we don't succeed, we give up
immediately! We then go to LR Gold which, in our case, reliably yields
better ultrastructure than the LR White. (and of course, we get much more
label)

All the above formulations are well known and can be found in any good
textbook. Sometimes I construct other formulations (for embedding
chocolate for paperweights for my co-workers) but those instances are
applicable only to what I am about and not of general interest to anyone.
'
Hope this is the answer you wanted.

Bye,
Hildy



From daemon Tue Apr 11 17:11:30 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Wed, 12 Apr 2000 13:57:36 -0500
Subject: Re: Epoxy formulations

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They must taste awful. And aren't they hard to unwrap?

Bob

Hildy wrote:

} Sometimes I construct other formulations (for embedding
} chocolate for paperweights for my co-workers)



From daemon Tue Apr 11 17:11:32 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 11 Apr 2000 14:59:12 -0500
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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This reminds me of a trick a Cambridge (yes, back then) service
engineer pulled to demonstrate vibration in the SEM room at a former
position. Fill a glass almost full and put it on the floor. Watch the
pretty rings. Do a quick calculation on the height of the waves -- if
they're 1mm high in the glass, at 10,000 times in the SEM they'd be
10 meters high. If any of the admin types have sail boats (or bass
boats, this being Oklahoma), this might make an impression on them.

Phil

} Phoebe:
}
} They want to put your lab next to a 4-lane highway...this is a joke, right?
}
} Larry ;-)
}
} PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} another suggestion: make some vibration measurements and
} check it out...
}
} } Microscopist:
} }
} } Our new lab is supposed to be built next to a four lane highway that has a
} } lot of 18 wheeler truck traffic. I am concerned about vibration problems
} } and would like to convince administration that the building should be built
} } on the other side of the property as far away from the highway as possible.
} } Any suggestions or known references covering this? Thanks in advance.
} }
} } Phoebe J. Doss
} } Manager/Adjunct Instructor
} } Electron Microscope Lab
} } Oklahoma State University
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory

}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue Apr 11 17:11:39 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 11 Apr 2000 17:01:53 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I can also tell you first hand about the effect on light microscopy and
microspectrometry equipment. I once had an assignment in a coking facility
which used a switch engine to run coal cars around the yars. Forget about
anything in the higher mag range!

Also, suggest that you talk to any of the EM apps people (Norm Burns, Tim
Maitland?) from the old Cambridge/Leica SEM group (now part of LEO,
Thornwood NY). Cambridge built that facility with a freight train running
through the back yard.

There are companies which will do site evaluations, including some of the
SEM groups. I don't know if there is a charge, but whatever it is, it
would be less expensive than (1) not being able to do research in a brand
new facility (administrators are traditionally very allergic to "egg on the
face" syndrome") or (2) having to move the lab to another location once it
is set up.

Hope this is helpful.

Best regards,
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



At 10:04 AM 4/11/00 -0500, Phoebe J Doss wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Apr 11 17:11:42 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 11 Apr 2000 17:34:00 -0500
Subject: Signal Conditioner Source?

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Hello All,

In order to maximize usefulness, I need a dc coupled signal conditioner
between
my WDS analog rate output and the SEM line scan input.

Small and battery powered would be nice... Am open to suggestion, but what
seems to be indicated is an amp with about +- 5 volt of INPUT offset with a
gain
of 1-25 (or more). Output swing should be capable of a minimum of +-5 volts.

(i.e. a couple of op-amps and 2 10- turn pots...)

I can build one, but would rather purchase if I can find one at a reasonable
price.
The MVA from my old ETEC would probably work, but is a NIM bin module and so
would need to be repackaged and powered to stand alone.

Thanks, Woody White
McDermott Technology, Inc.



From daemon Tue Apr 11 17:21:57 2000



From: Arnold, Jim :      jim.arnold6-at-honeywell.com
Date: Tue, 11 Apr 2000 17:12:24 -0500
Subject: Saving Measurements on photos

Contents Retrieved from Microscopy Listserver Archives
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I am currently taking measurement of SEM photos using IPP 4.1 (length
measurements, angles, and thickness)and I am wondering if there is a way to
"burn" these measurement into the photos for saving purposes. Has anyone
done this or am I missing something. Thanks in advance.


Jim Arnold
Senior Quality Technician / Failure Analysis
Honeywell International, Inc.
Aerospace Electronic Systems
Microelectronics and Technology Center
9140 Old Annapolis Rd
Columbia, MD 21045

email: jim.arnold6-at-honeywell.com
voice: (410) 964-4118
fax: (410) 992-5813




From daemon Tue Apr 11 17:21:57 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Tue, 11 Apr 2000 17:15:42 -0500
Subject: Yet one more horror story..........

Contents Retrieved from Microscopy Listserver Archives
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This one is from grad school days. I was doing my thesis on the
petrography and geochemistry of Miocene andesites as part of an
international study on PreHellenic arc volcanism. Since I did all my own
thin section preparations, I became the unpaid "volunteer" to keep the
prep lab in good running condition, and to assist other students. Nothing
compares to being an indentured servant. One day, a coastal sedimentary
graduate student approached me. He was working on temporal barrier
islands formed off the Gulf coast of Florida that formed as a result of
Hurricane Helena in 1986. His major advisor thought it would be good for
him make some impregnated thin sections from core samples, and stain them
for carbonates and feldspars, map the distribution of them. I instructed
him on all the staining procedures including safety
procedures.......emphasizing safety procedured since he would be dealing
with concentrated HF to etch the sections. I decided to stay in the lab to
do some maintenance, and to keep an eye on him. Good thing I did, for
what happened next could have turned into a real sad disaster. The student
knocked over his slide drying rack into the HF bath. Before I could say or
do anything, he immersed both of his hands into the concentrated HF to
save his sections. Fortunately for me, I had been on a volunteer rescue
squad in my teens, and was fully trained for all sorts of accidents. He
was lucky........didnt lose his fingers, but did lose his finger nails,
and his hands were scarred for life. Moral of this story? I should have
done the procedure myself. I took on a potentially grave situation under
my responsibility for a position I was not getting paid for, or properly
insured for by the department. I am glad the fellow didnt suffer worse for
his lack of thought, but I learned a valuable lesson, and held myself
accountable and responsible for what happened. The student didnt..........
Lou Solebello




From daemon Tue Apr 11 19:20:51 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Wed, 12 Apr 2000 09:14:12 +1000
Subject: Re: Critical Point Drying sandwich

Contents Retrieved from Microscopy Listserver Archives
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Has anyone devised some
} sort of holder (sandwich?) that might overcome this problem by
} keeping them flat during their trip through the dryer?
}
} We thank you in advance.
}
} Dick Briggs
} Biology Department
} Smith College
}
}
} you can make a sandwich using a small reusable swinnex filter holder
(holds 13 mm or larger polymer filters to filter liquids being expressed
from a syringe). You saw off the connecting bits leaving the bits which
screw together. Then you can sandwich things between two filters maybe
with a spacer made by including a gasket between the filters. Then you
process the whole assembly. Silver filters from Chuck Garber are good as
they wont dissolve in liquid CO2.....
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Tue Apr 11 19:20:52 2000



From: DrJohnRuss-at-aol.com
Date: Tue, 11 Apr 2000 19:27:17 EDT
Subject: Re: Saving Measurements on photos

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 4/11/00 6:44:48 PM, jim.arnold6-at-honeywell.com writes:

} I am currently taking measurement of SEM photos using IPP 4.1 (length
} measurements, angles, and thickness)and I am wondering if there is a way
} to "burn" these measurement into the photos for saving purposes. Has anyone
} done this or am I missing something. Thanks in advance.

With IPP you can label each feature with its measurement value for one
measurement parameter.



From daemon Tue Apr 11 19:20:53 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 12 Apr 2000 00:40:37 +0100
Subject: Turbo pump horror

Contents Retrieved from Microscopy Listserver Archives
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Several years ago, one of my research students was using the
Cambridge S250 SEM on an early solo session. The room was
hushed, in almost in total darkness, and he was giving his total
concentration to the screen while he adjusted the image. As he
moved his hand to the specimen stage controls the turbo-molecular
pump disintegrated without warning, making a crash that sounded
like a metal tray full of spanners being dropped from a great height,
followed immediately by the wailing of alarms. The poor chap was
literally green with shock - he thought he had caused it!

When the column was opened up a glittering cloud of aluminium
alloy powder drifted out. The turbo pump - a double-ended model -
had its rotors and stators intertwined so forcibly that there was no
free play. Presumably it had come to rest from 60,000 rpm in less
than a single rotation. That works out at a damage rate of almost
1billion dollars per second!

Chris


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Apr 11 19:31:31 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 11 Apr 2000 19:24:30 -0500
Subject: pre-final horror story?

Contents Retrieved from Microscopy Listserver Archives
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} } } From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: pre-final horror story?
} } Send reply to: c.jeffree-at-ed.ac.uk
} } Date sent: Tue, 11 Apr 2000 16:01:02 +0000
} }
} } Several years ago, one of my research students was using the
} } Cambridge S250 SEM on an early solo session. The room was
} } hushed, in almost in total darkness, and he was giving his total
} } concentration to the screen while he adjusted the image. As he
} } moved his hand to the specimen stage controls the turbo-molecular
} } pump disintegrated without warning, making a crash that sounded
} } like a metal tray full of spanners being dropped from a great height,
} } followed immediately by the wailing of alarms. The poor chap was
} } literally green with shock - he thought he had caused it!
} }
} } When the column was opened up a glittering cloud of aluminium
} } alloy powder drifted out. The turbo pump - a double-ended model -
} } had its rotors and stators intertwined so forcibly that there was no
} } free play. Presumably it had come to rest from 60,000 rpm in less
} } than a single rotation. That works out at a damage rate of almost
} } $1billion per second!
} }
} } Chris
} } ------- End of forwarded message -------
} } =====================================================================
} } DR CHRIS JEFFREE
} } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} } UNIVERSITY OF EDINBURGH
} } Daniel Rutherford Building
} } King's Buildings, Mayfield Road
} } EDINBURGH, EH9 3JH, Scotland, UK
} } Tel. #44 131 650 5345
} } FAX. #44 131 650 6563
} } Mobile 0410 585 401
} } email c.jeffree-at-ed.ac.uk
} } SEM / TEM bookings sem-at-ed.ac.uk
} } =====================================================================
}
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Apr 12 08:02:00 2000



From: Witoon Ngow :      witoon-at-su.ac.th
Date: Wed, 12 Apr 2000 08:59:03 -0700 (GMT)
Subject: unsubscribe

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unsubscribe witoon-at-su.ac.th




From daemon Wed Apr 12 08:02:04 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 12 Apr 2000 14:17:47 +1000
Subject: RE: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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Maybe I should have headed this "funny horror stories". I used to run an EM
Unit adjacent to a geology department. One of their more intellectual types
decided that the only place to operate a rock-shaking machine was up against
the outside wall of the darkroom and the SEM rooms. I never bothered to check
the effects on the SEM, but observed that through the enlarger's focus
magnifier the image was dancing. I told the grad student users that the shaker
had to go, complete with reasons. Nothing happened, then I just turned the
shaker off whenever it was on.

Eventually I was confronted by that intellectual quietly asking: "why was I
against his shaker"?
I figured years ago that anger was unprofessional and non-productive, but I
lost it on that occasion. I asked rhetorically, how can you call yourself an
intellectual, when you are unable to work out why a two bob (nickel and dime)
shaker had no place next to an EM Unit. That shaker disappeared on next day.

Maybe I should have sent him an exam to sort out the problem:
My instruments were there first
Put the respective instrument cost into the equation
Consider the difficulty involved in moving his shaker versus rehousing the EM
Unit
Also consider that a heavy shaker is almost designed to produce vibration. A
microscope magnifies and not just objects but vibrations too. So one um of
actually transmitted movement 50000x enlarged is 50mm - I trust that the
movement was less than 1um.

In relation to the highway, it must be noted that you cannot switch that off.
Also its difficult to foretell how much vibration would be transmitted. Its a
question of risk: are those administrators willing to relocate the unit later
or will they find the money to place several instruments on expensive
antivibration devices.
I don't believe its worth the risk; convince them.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

}
}
} Phoebe:
}
} They want to put your lab next to a 4-lane highway...this is a joke, right?
}
} Larry ;-)
}
} PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} another suggestion: make some vibration measurements and check it
} out...
}
} }
} } Microscopist:
} }
} } Our new lab is supposed to be built next to a four lane highway that has a
} } lot of 18 wheeler truck traffic. I am concerned about vibration problems
} } and would like to convince administration that the building should be built
} } on the other side of the property as far away from the highway as possible.
} } Any suggestions or known references covering this? Thanks in advance.
} }
} } Phoebe J. Doss
} } Manager/Adjunct Instructor
} } Electron Microscope Lab
} } Oklahoma State University
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov



From daemon Wed Apr 12 08:02:10 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 12 Apr 00 00:03:17 -0700
Subject: Re: vibration due to truck traffic?

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: Re: vibration due to truck traffic?
Do this trick with a dish of water but reflect a beam of light off the water surface onto a wall and the vibrations are magnified before your eyes.

Paul Webster

Mssage from Phil Oshel:

This reminds me of a trick a Cambridge (yes, back then) service engineer pulled to demonstrate vibration in the SEM room at a former position. Fill a glass almost full and put it on the floor. Watch the pretty rings. Do a quick calculation on the height of the waves -- if they're 1mm high in the glass, at 10,000 times in the SEM they'd be 10 meters high. If any of the admin types have sail boats (or bass boats, this being Oklahoma), this might make an impression on them.

Phil




From daemon Wed Apr 12 08:02:12 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 11 Apr 2000 21:38:48 -1000 (HST)
Subject: another turbo horror story

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If it ain't broke, don't fix it. This is the lesson I learned the hard
way.

Several years back I was looking something up in the Balzers 400 freeze
fracture manual, and noticed it recommended strongly that the turbo pump
be sent in for reconditioning every 50,000 hours of use (or some such
number). I had no previous experience with turbo pumps, and the
consequences sounded pretty dire, so I was concerned. Since it had run
24/7 for several years, and then off and on for several more, it easily
had whatever number of hours. The facility director was getting ready for
a big project utilizing the instrument and, although he was reluctant, I
convinced him that we should send the turbo pump in before he started.

It came back a few weeks later, and it was clearly not our pump, but
another reconditioned one. As I lifted this large pump out of the box two
small ball bearings bounced onto the floor and rolled away. Still holding
the pump, I watched them go. Then I turned the pump all around in my
arms, looking for any signs of damage or loose parts, but all looked
fine. I considered calling the company, but it was Friday and with the
time difference, it would be days before I got an answer. I figured what
the heck, either it is going to work or not! So I installed it and turned
it on, standing as far away as I could. It started up fine and achieved a
reasonable vacuum, so I left it running over the weekend. Monday
afternoon I decided it was OK, and turned it off.

My first thought was that a jet had crashed into the wall behind me and
that I was going to die. And from the look on the faces of the others in
the lab, they clearly thought they were going to, as well. The horrible
screeching noise actually stops really suddenly as those pumps seize up,
and then the quiet is deafening.

I opened the chamber to find it full of aluminum glitter.

The pump was replaced.

When I talked to some EM service people who had a lot more experience with
turbo pumps, they all seemed to think that one should never overhaul a tp,
but just wait until it crashes. Sure, it's a lot more expensive to repair
it then, but apparently few do fail within the normal lifetime of the
instruments they are on. Sigh.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Apr 12 08:02:17 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 12 Apr 2000 05:42:08 -0500
Subject: Re: Saving Measurements on photos

Contents Retrieved from Microscopy Listserver Archives
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} In a message dated 4/11/00 6:44:48 PM, jim.arnold6-at-honeywell.com writes:
}
} } I am currently taking measurement of SEM photos using IPP 4.1 (length
} } measurements, angles, and thickness)and I am wondering if there is a way
} } to "burn" these measurement into the photos for saving purposes. Has
anyone
} } done this or am I missing something. Thanks in advance.
}
} With IPP you can label each feature with its measurement value for one
} measurement parameter.
}
Years ago I used potassium iodide, calcium chloride and copper sulfate
as a bleach. I don't remember the formula but it is not very critical. You
could use this stuff to mark any silver photographic image. I think the
silver ends up a silver iodide so you would need to refix and wash them.
You would probably want to add a gelling compound to it to keep it from
running starch or wall paper paste would be a good place to start.

A simpler method would be to scratch the film or use India Ink on it.
Magic Marker felt tip pens should work as well. There might be some
bleeding on the emulsion side.

For temporary marking there are opaquing paints to cover pin holes
on litho negatives that is water based and should wash off the base
side just fine. You nearest print shop or graphics art supply can fix
you up with a life time supply for 5 bucks.

Good Luck
Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Apr 12 08:02:18 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 12 Apr 2000 05:52:27 -0500
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} } } Our new lab is supposed to be built next to a four lane highway that has
a
} } } lot of 18 wheeler truck traffic. I am concerned about vibration
problems
} } } and would like to convince administration that the building should be
built
} } } on the other side of the property as far away from the highway as
possible.
} } } Any suggestions or known references covering this? Thanks in advance.
} } }

I take it you are going in on Hall of Fame. It is not as bad as you
fear but it is bad.

Starting from scratch an air supported floor for the room would not be
too expensive if ou designed it your self. Twenty Five cent pre pound steel
and a decient air pump will handle low frequencie vibratin and active
stuff does a great job from 10HZ up.

Now if you could just move a cross the road to the sheep fram most of
your problems wold dissappear.

There are several minds on campus that have delt with simular situations
and I will be glad to get you togeather.

Good luck
Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Wed Apr 12 08:02:23 2000



From: HARRISm-at-esm-semi.co.uk
Date: Wed, 12 Apr 2000 10:33 +0000 (GMT)
Subject: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and staining
methods are used to obtain and or enhance selected features prior to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing capabilities
of image processing at my fingertips it's possible to ' modify' and
possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of different
sem capabilities / preparation methods / etches or are they simply
'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot believe
what you see ?

Regards.





From daemon Wed Apr 12 08:02:24 2000



From: Andrew Vogt :      andrew.d.vogt-at-abbott.com
Date: Wed, 12 Apr 2000 07:20:23 -0500
Subject: Turbo pump horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Was the cause ever determined? Or did I miss it somewhere in the text?

An excellent way to stop a turbo without going through the proper shut down
sequence is to drop something into the turbines. An unwitting young prof at
the grad school I went to inadvertantly dropped I can't remember what in a
turbo pump in the UHV system that he was designing. This was with the pump
running at several hundred thousand rmp. You can imagine the size of this
pump. I am not sure that it stopped in less than one rotation, but it fried
the turbo, non pun intended. He had to replace it more so because he
borrowed the pump from another prof. than because he needed one for his
experiments. You can image how green he was.

The moral of this story is to cover the inlet with a screen. The chances of
anything falling into it is significantly reduced and you can save thousands
of dollars.

Regards to you all,
Andy





cjeffree-at-srv0.bio.ed.ac.uk on 04/11/2000 09:55:57 PM
To: microscopy-at-sparc5.microscopy.com-at-INTERNET
cc:


Several years ago, one of my research students was using the
Cambridge S250 SEM on an early solo session. The room was
hushed, in almost in total darkness, and he was giving his total
concentration to the screen while he adjusted the image. As he
moved his hand to the specimen stage controls the turbo-molecular
pump disintegrated without warning, making a crash that sounded
like a metal tray full of spanners being dropped from a great height,
followed immediately by the wailing of alarms. The poor chap was
literally green with shock - he thought he had caused it!

When the column was opened up a glittering cloud of aluminium
alloy powder drifted out. The turbo pump - a double-ended model -
had its rotors and stators intertwined so forcibly that there was no
free play. Presumably it had come to rest from 60,000 rpm in less
than a single rotation. That works out at a damage rate of almost
1billion dollars per second!

Chris


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~






From daemon Wed Apr 12 08:02:24 2000



From: Frank Shapiro :      franks-at-netcom.ca
Date: Wed, 12 Apr 2000 08:19:15 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good trick Paul, but in my checkered past, we used a large (12" diameter) dish filled with mercury. That gave much better reflection of the light and very distinct vibration patterns on the wall.

Cheers,

Frank Shapiro.

Paul Webster wrote:

}
} Do this trick with a dish of water but reflect a beam of light off the water surface onto a wall and the vibrations are magnified before your eyes.
}
} Paul Webster
}
} Mssage from Phil Oshel:
}
} This reminds me of a trick a Cambridge (yes, back then) service engineer pulled to demonstrate vibration in the SEM room at a former position. Fill a glass almost full and put it on the floor. Watch the pretty rings. Do a quick calculation on the height of the waves -- if they're 1mm high in the glass, at 10,000 times in the SEM they'd be 10 meters high. If any of the admin types have sail boats (or bass boats, this being Oklahoma), this might make an impression on them.
}
} Phil






From daemon Wed Apr 12 08:14:39 2000



From: Kristin A Breen :      kristin_a_breen-at-email.mobil.com
Date: Wed, 12 Apr 2000 08:03:00 -0500
Subject: VIbrations - consultant

Contents Retrieved from Microscopy Listserver Archives
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Phoebe,

You may want to take a few minutes and peruse the following site:

http://www.vibeng.com/index.htm

Vibration Engineering Consultants -VEC- has some useful information regarding
site selection, vibration, EM and acoustic interferences, etc.
However, it may help you more to talk with them personally. Craig Franklin
came
to our site in southern New Jersey a few years ago, to survey
potential new locations for our SEM, and made some recommendations, as far as
location, field cancellation and vibration insulation. We ended up
buying the AC/DC field canceling system that he recommended, and a vibration
table. These items have been a huge help in insuring the continued high
resolution capabilities of our SEM. The people at VEC may be able to help make
your case to your management, before the new facility is built.

Kristin Breen
Staff Chemist, Marketing Technical Services Lab
North American Region
ExxonMobil Lubricants and Petroleum Specialties Co.
Paulsboro, NJ (856) 224-2864




From daemon Wed Apr 12 14:43:32 2000



From: anderron-at-us.ibm.com
Date: Wed, 12 Apr 2000 09:31:46 -0400
Subject: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



There is a difference between unwittingly taking pictures of artifacts and
fraudulent digital manipulation. In the latter case there have been
several strings on this listserver and elsewhere on digital manipulation,
see the archives. Essentially, digital manipulation of image contrast,
brightness, and gamma to produce a better image is the same as dodging,
burning-in and choosing different contrast grade papers in an old-fashioned
darkroom to enhance but not alter an image, this is OK. Beyond that, it is
all to easy to alter an image digitally. "Alter," in the fraudulent sense.
I don't see anything wrong with deleting specimen preparation
artifacts--scratches, left-over polishing compound, etc.--as long as the
true image content is unaffected. What is and what isn't in this category
is a judgement call on my part. I assume that, unless proven otherwise,
all of my colleagues out there are doing the "right" thing and clearly
stating what manipulations of the image content were performed and why.

With all due respect, Martyn, if I were you I'd worry more about the former
case. SEM imaging of chemically etched integrated circuit cross sections
is horrendously prone to artifact production. We stopped this practice
more than a decade ago, tuning instead to high-angle ion milling for short
time periods. See our paper: Martinek, et al., 1989 MSA Proceedings, p.
720. We've been using GATAN Duo Mill ion millers for this from our TEM
areas. GATAN has brought out a unit specifically to perform this function
for SEM labs. It's the Model 682 (?) PECS or, the new PECS + RIBE system.
(The question mark is mine--I'm not sure of the model number of the simple
PECS system.) Good luck.

Ron

I have no business or financial relationship with GATAN other than being a
long-time satisfied customer.



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



"HARRISm/-at-esm-semi.co.uk" on 04/12/2000 06:33:00 AM

To: Microscopy-at-sparc5.microscopy.com
cc:



A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and staining
methods are used to obtain and or enhance selected features prior to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing capabilities
of image processing at my fingertips it's possible to ' modify' and
possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of different
sem capabilities / preparation methods / etches or are they simply
'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot believe
what you see ?

Regards.









From daemon Wed Apr 12 14:43:32 2000



From: Ford M. Royer :      froyer-at-bitstream.net
Date: Wed, 12 Apr 2000 08:42:57 -0500
Subject: Re: Tissue Tek machine evaluation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The TissueTek* brand of histology equipment has been around for many years, and
in my opinion, is at the top of the quality, dependability, and reliability
lists. The confusion here is what exact "TissueTek" models you are
considering. Even using the suffixes "II" and "III" does not completely
identify which TissueTek product you are talking about. It is best to acquire
and use the individual Model numbers to prevent confusion.

What I call a "TissueTek II" processor is the [Model 4640]. This is a basic
"dip and dunk" rotary processor and is considered an "open system" (no fume
control). It is still being manufactured and it can be purchased as a new piece
of equipment. No problem getting spare parts. You would have to provide some
type of fume hood for it. The 4640 is compact enough that it would fit inside a
standard chemical fume hood. If you would only be using it on a random basis
and low volume, this would be a good choice. It can hold up to 120 specimens
per run.

I consider the "TissueTek III" [Model 4660] as the first enclosed tissue
processor with on-board fume control. It is also known as the "V.I.P."
However, this Model was discontinued in 1982-83 and is totally OBSOLETE as far
as spare parts and support from the manufacturer. There are a number of these
units still in service around the world, however.

} From approximately 1983 to 1994 the next generation TissueTek V.I.P.s were the
"K" series with [Model Numbers: 4617, 4618,4619]. They came in three volume
sizes and and could be purchased in either a bench-top or floor configuration.
They still supported by the manufacture, and spare parts are readily available.
These, once again, are "closed systems" with fume control.

The current production models of the "V.I.P." is the "E" series [Models: 4890 &
4894] in the bench top configuration, and [Models 4892 & 4896] in the floor
model configuration. They come in two volume sizes.

Of the above, the only processor that I would not recommend that you consider is
the original TissueTek III VIP [Model 4660].

I hope this answers your questions.

* TissueTek, and TissueTek V.I.P. are registered trademarks of Sakura Finetek,
Inc.

--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
phone: 800-565-1895, Ext. 17
fax: 612-929-1895
Email: froyer-at-bitstream.net
web site: http://www.aibltd.com

"A.P. Alves de Matos" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Thanks Norm for your suggestions
}
} I will be more specific.
} I will need to process up to 60 specimens/week in two or three separated
} weeks during the year. The rest of the time hand processing is OK. Since the
} specimens are prone to dammage upon storage (immunohistochemical techniques
} on sponge tissues), I do not want to store them to facilitate processing.
} So, it is important to know if the machine can handle this amount of work.
} I have one technician that has many other things to do.
} Basic assistence should be available from our technical department. Anyhow I
} would not trust newer instruments just because they are more recent. I know
} of (and have) a few super-new machines (amaizingly expensive) that had to be
} thrown to garbage because no one (assistence included) was able to put them
} to regular work. It is important to know if the machine model is a well
} tested one that usually works for years with no problems or if it has
} regular problems.
} Since histology and histopathology labs of my knowledge use other tissue
} processors, I was not able to get specific advice locally about these
} machines.
}
} Thanks
} A.P. Alves de Matos
}
} ----- Original Message -----
} } From: Norm Granholm {granhona-at-email.uc.edu}
} To: A.P. Alves de Matos {apmatos-at-ip.pt}
} Sent: Tuesday, April 11, 2000 12:57 PM
} Subject: Re: Tissue Tek machine evaluation
}
} } Dr. de Matos:
} }
} } I believe some general guidelines are appropriate for you to consider:
} } 1. Get the newest machine you can afford. Older machines will have
} more
} } maintenance requirements and will become obsolete sooner.
} } 2. Is instrument repair readily available? If not then any
} automated
} } system is potentially a problem. Automated systems do break.
} } 4. Your note says "lots of paraffin embeddings". What is "lots"?
} Hand
} } processing is quite feasible for batches of small numbers and if personnel
} help
} } is not limiting (see next). No one likes doing it and an automated system
} lets
} } skilled individuals perform more appropriate tasks. All of this is,
} however, an
} } issue of resource utilization. You have considered these matters already,
} } undoubtedly.
} } 3. If personnel help is a limiting factor, the more automated the
} better
} } for you. If personnel help is not a limiting factor, then the less
} automated the
} } better for you. This may sound strange but I believe it is true. It all
} depends
} } upon who is paying the bills and whether the funds are available for other
} uses.
} } And there are always other uses for funds.
} }
} } Having raised all of those points, I'll tell you that I used a Tek II when
} they
} } first came out ( now some 20 years ago). And this for fewer than a dozen
} samples
} } per week. I wanted skilled individuals to do other tasks than hand dip
} tissues.
} }
} } You might look, also, at Leitz/Leica tissue processors. My experiences
} with
} } Leica are that they provide outstanding equipment at reasonable prices.
} Often
} } they have trade in items available. Were I to have to chose I would head
} this
} } way.
} }
} } Best wishes,
} }
} } Norm
} } (Norman.Granholm-at-uc.edu)
} } Pathology, Univ. Cincinnati
} }
} } Voice 513 558 0182
} } Digital Pager 513 249 3889
} } ===============================



From daemon Wed Apr 12 14:43:33 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 12 Apr 2000 08:47:42 -0500
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What's funny?Ê My lab is on the 4th floor of a semiconductor fab at the intersection
of two 6-lane freeways.Ê We have 4 SEMs, 3 focused ion beams, and 2 200kV TEMs.Ê We
routinely work over 150KX (SEM) with very few problems.Ê It's a well-designed
building.Ê EMI is more of a problem.

Larry Allard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} ToÊ Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Phoebe:
}
} They want to put your lab next to a 4-lane highway...this is a joke, right?
}
} LarryÊ ;-)
}
} PSÊ my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} ÊÊÊÊÊÊ another suggestion:Ê make some vibration measurements and check it out...
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } ToÊ Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Microscopist:
} }
} } Our new lab is supposed to be built next to a four lane highway that has a
} } lot of 18 wheeler truck traffic.Ê I am concerned about vibration problems
} } and would like to convince administration that the building should be built
} } on the other side of the property as far away from the highway as possible.
} } Any suggestions or known references covering this?Ê Thanks in advance.
} }
} } Phoebe J. Doss
} } Manager/Adjunct Instructor
} } Electron Microscope Lab
} } Oklahoma State University
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413Ê Fax
} allardlfjr-at-ornl.gov

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky HoldfordÊ (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB
Kilby Center West
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Ê



From daemon Wed Apr 12 14:43:33 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 12 Apr 2000 09:50:14 -0700
Subject: Phys/Mat: Looking for Bloch Wave Paper

Contents Retrieved from Microscopy Listserver Archives
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Hi There,

I'm trying to track down a paper presented in the early '70s at an EM
meeting in Erice, Italy. The papers were published in a volume called
Electron Microscopy in Materials Science Vol. II, Valdre and Ruedl,
eds. The paper I'd like to get is one by A. Metherell on Bloch waves.
My quest has stymied our interlibrary loan folks here (I did get the 3rd
vol). Anyone got a Vol II or the Metherell paper?

Thanks

John

John Heckman
MSM Department
Michigan State University





From daemon Wed Apr 12 14:43:34 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 12 Apr 2000 10:10:38 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Becky:

I wouldn't expect much vibration either, if I worked in a Billion $
building...Phoebe, what was your budget again?

Larry ;-)




} What's funny? My lab is on the 4th floor of a semiconductor fab at
} the intersection
} of two 6-lane freeways. We have 4 SEMs, 3 focused ion beams, and 2
} 200kV TEMs. We
} routinely work over 150KX (SEM) with very few problems. It's a well-designed
} building. EMI is more of a problem.
}
} Larry Allard wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Phoebe:
} }
} } They want to put your lab next to a 4-lane highway...this is a joke, right?
} }
} } Larry ;-)
} }
} } PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} } another suggestion: make some vibration measurements and
} check it out...
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Microscopist:
} } }
} } } Our new lab is supposed to be built next to a four lane highway that has a
} } } lot of 18 wheeler truck traffic. I am concerned about vibration problems
} } } and would like to convince administration that the building
} should be built
} } } on the other side of the property as far away from the highway
} as possible.
} } } Any suggestions or known references covering this? Thanks in advance.
} } }
} } } Phoebe J. Doss
} } } Manager/Adjunct Instructor
} } } Electron Microscope Lab
} } } Oklahoma State University
} }
} } Dr. Lawrence F. Allard
} } Senior Research Staff Member
} } High Temperature Materials Laboratory
} } Oak Ridge National Laboratory
} } 1 Bethel Valley Road
} } Bldg. 4515, MS 6064
} } PO Box 2008
} } Oak Ridge, TN 37831-6064
} }
} } 865-574-4981
} } 865-576-5413 Fax
} } allardlfjr-at-ornl.gov
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 972-598-1291 (pager)
} KFAB Physical Analysis Lab--SEM/FIB
} Kilby Center West
} Texas Instruments, Inc.
} Dallas, TX
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed Apr 12 14:43:34 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 12 Apr 2000 10:34:14 -0400
Subject: Re: another turbo horror story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina Carvalho wrote:

} When I talked to some EM service people who had a lot more experience with
} turbo pumps, they all seemed to think that one should never overhaul a tp,
} but just wait until it crashes. Sure, it's a lot more expensive to repair
} it then, but apparently few do fail within the normal lifetime of the
} instruments they are on. Sigh.

Dear Tina,
Yes, turbopumps canibalize themselves spectacularly--they're almost as much

"fun" as high-pressure Hg-vapor lamps. I'd like, however, to put in a good
word
for servicing them on a regular basis. The turbos that were on the HVEM when
I got here were ~200 lbs, turned at ~10,000 rpm, and occasionally ate
themselves.
We soon replaced them with the TPU330 model pumps, which are ~30 lbs., turn
at ~15,000 rpm, and have been humming along at "standby" speed for years. The
vacuum is as good at standby as at full speed, so we have seen no need to go to
the
higher speed. We've changed the oil on a regular schedule and sent the pumps
to
Balzers every two years for bearing changes. There has been no deterioration
in
performance for ~15 years now.
Yours,
Bill



From daemon Wed Apr 12 14:43:35 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Wed, 12 Apr 2000 10:49:05 -0400
Subject: Job opportunity - reposted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cell Biologist/Microscopist

SmithKline Beecham, a world class leader in Research and Development, continues
to pioneer innovative pharmaceutical and healthcare products and services. We
have the following opportunity available at our state-of-the-art suburban
Philadelphia facility. Working in our Safety Assessment department you will
provide technical support and scientific input into the design and execution of
studies to elucidate the cellular mechanisms of drug-induced toxicities. You
will prepare biological specimens for transmission and scanning electron
microscopy, confocal microscopy, X-ray microanalysis and immunohistochemistry.
You will also perform qualitative and quantitative data analysis using computer
assisted image analysis systems. We require a BS/MS in a biological science, and
3-5 years of microscopy experience in cell biology, physiology, toxicology.
SmithKline Beecham is dedicated to an innovative workplace and supports you with
career long opportunities and learning. We offer a competitive benefits and
compensation package. For confidential consideration, please forward your
scannable resume to:
SmithKline Beecham
Attention: Human Resources
AD CODE: 2K0325W
c/o National Resume Processing
P.O. Box 1070
Burlington, MA 01803 USA

Indicating ad code is essential. Principals only, no agencies, please. For a
full listing of current opportunities, or to submit a resume online, visit our
website at www.sb.com/careers.

**********************************************************************************************

Please respond directly to the address in the advertisement, and not to me.

Regards,
Bev Maleeff




From daemon Wed Apr 12 14:43:37 2000



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Wed, 12 Apr 2000 11:08:59 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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Hallo magnetic Microscopists !

Everyone's been discussing vibration in this thread
(talk about slavish adherence to topic ...) but there's
another consideration - magnetic disturbances. Those
truck have steel frames, so they'll disturb the Earth's
magnetic field as they pass by. I inadvertently made a
magnetometer once, and I could see the inflence of
vehicles passing by fifty feet away on my oscilloscope
screen. At M.I.T. there was a lot of concern about the
nearby elevator in one EM lab ...

Best regards,
George Langford, Sc.D., who's got no vested interest in
Earth's magnetic field except when out hiking.
amenex-at-amenex.com



From daemon Wed Apr 12 14:43:42 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 12 Apr 2000 13:13:51 -0400
Subject: SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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Dear Martyn:

Several years ago (MSA in Cleveland if I recall correctly), there was some
sort of roundtable discussing the ethics of image manipulation. I can't
remember much more than that, but perhaps those with a better memory who
may have participated in the discussion would have some good input.

Best regards-

David
Writing at 9:53:20 AM on 04/12/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by
INTERNET:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
} A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and staining
methods are used to obtain and or enhance selected features prior to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing capabilities

of image processing at my fingertips it's possible to ' modify' and
possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of different

sem capabilities / preparation methods / etches or are they simply
'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot believe
what you see ?

Regards.

{



From daemon Wed Apr 12 14:43:49 2000



From: Mitch McCartney :      Mitch.McCartney-at-alconlabs.com
Date: Wed, 12 Apr 2000 14:31:40 -0500
Subject: Position

Contents Retrieved from Microscopy Listserver Archives
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Scientist
Retina Research - Degenerative Disease

Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle,
S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the
global leader in the discovery, development, manufacture and marketing of
ophthalmic pharmaceuticals and medical devices. Alcon expects to double its
sales over the next five years and has achieved a profit growth rate of
approximately 11% over the last several years. Historically, the company
commits 10% of sales to research and development. Products developed in the
last ten years generate 50% of current sales. The current product pipeline
is strong. Alcon was recently renamed to the Fortune List of the 100 Best
Companies to Work for in America.

As the successful candidate, you will conduct research in the area of
retinal degeneration aimed at identifying novel chemical agents to prevent
neurodegenerative disease using histopathologic and cell culture techniques.
You will participate in the design and provide expertise in preparation and
histopathologic evaluation of plastic and paraffin embedded retinal tissue,
including immunocytochemical techniques. You will also provide technical
expertise in cell/tissue based retina models; participate in handling,
manipulating, and dosing of various laboratory species; and interpret,
summarize, and communicate experimental results in appropriate formats.

Qualified candidates will have 1) at least nine years of applicable
experience, probably in a hospital or university laboratory, following
receipt of a B.S. in biology or a related discipline, or 2) at least three
years of applicable experience followed by receipt of an M.S. with thesis in
biology or a related discipline. Demonstrated competency in histology
techniques is required. Experience in cell/tissue culture is a plus.

Alcon professionals enjoy state-of-the-art facilities in a year-round
business casual environment. Our company offers competitive salaries and a
wide array of excellent benefits: a very generous retirement plan and
dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life,
and accident insurance, death, dismemberment, illness and disability
benefits, tuition reimbursement, employee credit union, adoption assistance,
dependent care, and wellness programs, on-site fitness center, running
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An Equal Opportunity and Affirmative Action Employer. Pre-employment drug
testing.

Please forward your salary requirements and your resume to:

Job64_1261-at-careers.alconlabs.com
Reference Code: SRR





From daemon Wed Apr 12 18:42:40 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 12 Apr 2000 13:39:07 -0700
Subject: RE: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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Martyn Harris writes ...

} ...
} Question.
}
} In semiconductor failure investigation various
} etchants and staining
} methods are used to obtain and or enhance selected
} features prior to
} FESEM investigation and image capture .
} As I now capture images digitally and have the
} increasing capabilities
} of image processing at my fingertips it's possible to
} ' modify' and
} possibly distort the initially accurate image obtained
} which could
} lead to misleading results .
}
} I receive people's images and wonder if they are a
} result of different
} sem capabilities / preparation methods / etches or are
} they simply
} 'touched up '. They probably think the same about mine .
}
} ...

Surely you are not implying similar distortions were never a
possibility in the wet darkroom??

It is possible to put (for example) a fine checkerboard pattern in a
small box which would imply this image has never been subjected to
"blur", "sharpening", or many other kernal operations ... or you could
install a standard grayscale to imply brightness, contrast and gamma
are original ... BUT, you would need trust the author didn't install
the markers after distorting.

=shAf= :o)



From daemon Wed Apr 12 18:42:40 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 12 Apr 2000 15:45:53 -0500
Subject: RE: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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It's an old problem. A darkroom is a good tool
for image manipulation too - just less handy and
needs more experience.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: "HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
} [mailto:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com]
} Sent: Wednesday, April 12, 2000 5:33 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Subject : SEM + DIGITAL IMAGING
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} A general enquiry regarding integated circuit X section images :
}
} Email . harrism-at-esm-semi.co.uk
} Name . Martyn Harris Device Engineering - failure analysis
}
}
} Question.
}
} In semiconductor failure investigation various etchants
} and staining
} methods are used to obtain and or enhance selected
} features prior to
} FESEM investigation and image capture .
} As I now capture images digitally and have the
} increasing capabilities
} of image processing at my fingertips it's possible to '
} modify' and
} possibly distort the initially accurate image obtained
} which could
} lead to misleading results .
}
} I receive people's images and wonder if they are a
} result of different
} sem capabilities / preparation methods / etches or are
} they simply
} 'touched up '. They probably think the same about mine .
}
} Therefore is there any way of controlling image
} processing to enable
} like for like comparison ? or
} any international standards ? way of indicating on an
} image it has
} been subject to processing or is it now a case of you
} cannot believe
} what you see ?
}
} Regards.
}
}
}
}



From daemon Wed Apr 12 18:42:43 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 12 Apr 2000 16:47:51 -0500
Subject: Last call for parts

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We have disconnected our old Philips EM-200 and will send it to salvage within the next week. If anyone wants parts from it or the others we have in storage (used for parts) please contact me immediately. All used parts (other than vacuum tubes) are free for the asking but you will need to pay shipping.
We also have a Reichert OMU-3 ultramicrotome which can be used for parts. It is not functional at the moment.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Wed Apr 12 18:42:46 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Apr 00 15:45:21 -0700
Subject: FEBS Practical Course

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Course Announcement:

FEBS Stereology and Immunocytochemistry course to be held at the University of Oslo in Norway. For details see: http://www.hei.org/htm/curs.htm

This is an intensive practical course covering all aspects of specimen preparation for immunocytochemistry. The practical part of the course is supported by in-depth lectures explaining preparation protocols and their theoretical background.

Subjects covered include stereology (quantitation), chemical fixation, rapid freezing, freeze substitution, cryoultramicrotomy, specimen contrasting, antibody labeling, pre-embedding labeling, antibody and colloidal gold preparation, and specimen evaluation.

Participants are encouraged to bring their own samples.

Due to the intensive practical nature of this course places are limited, so apply early.
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Apr 12 18:42:47 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Apr 00 16:11:27 -0700
Subject: vibration due to traffic

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Having gone through a similar problem with vibrations, I can fully appreciate what might happen. However, I cannot give you any formal information for you to support your cause, I can only offer you my story.

I moved to my insitute to set up an imaging laboratory but before I arrived, a vibration survey had been performed on the site chosen for the EM's. This site passed inspection.

When I arrived, I looked at the site and suggested it might not be suitable. I was instantly presented with the very professional survey file which said the site was suitable for electron microscopes, (as were about 10 other locations throughout the building).

When the EM supplier came in to survey the site, their standards were very different to the generic vibration expert, and the site failed (not surprizing considering it was in the middle of the 4th floor!). Every other site we looked at failed too, so we were stuck with attempting to make things work using an anti-vibration platform under the first microscope we installed (a TEM). Amazingly, the supplier of the anti-vibration platform did not even come to look at the site when I contacted them. They seemed to have no intention of doing a site survey, and were not even interested in obtaining information from the microscope supplier about the sort of problem we were attempting to correct.

We put the TEM on the very expensive air table, which made it 8' taller, and thus more difficult to operate, and tested it. The platform did not help at all in isolating the low frequency vibration that seemed to be running through the building. It seems that if the vibrating frequency is low enough, even the anti-vibration platform moves! It was also very difficult to operate a machine you couldn't touch when taking a picture.
Eventually we found a stable site, renovated it and installed the microscopes there. All is now in order and the microscopes are performing to specification. Interestingly, the space is next to an in-door parking lot but the flow of traffic, although noticable to people in the lab, does not affect the microscopes. These are mostly slow moving cars with a few SUV's and pick-up trucks shaking the ground occassionally. My guess is that this is because the microscopes are installed between closely spaced support pillars on the side of the building. (Can't wait for the big earthquake to shake them up a bit!)

My advice would be to explain just how sensitive the EM's are to vibration, especially the low frequency shaking you can get from moving trucks and trains, and that the laboratory be located in as quiet a place as possible. Make sure that any vibration surveys are carried out by EM specialists, not by local heros, and keep away from floating platforms.

I am willing to re-tell ALL my experiences with sorting out our problem to anyone who wants or needs to listen. Correcting our mistakes was VERY costly.

Regards,

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Apr 13 00:38:25 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Wed, 12 Apr 2000 18:48:39 -0500
Subject: trucks aren't the only problems

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I thought about adding this story to the horror list but now it
seems even
more appropriate for the truck vibration problem. I quote directly from the
book "Principles and Practise of Electron Microscope Operation" by Agar and
Chescoe: The main sources of trouble, which can directly affect the
resolution obtained with the instrument, are mechanical vibrations and
electric fields.
Several years ago we set up an EM facility in the basement of our new,
state of the art, 5 story building here at Stanford. The engineers
surveyed the room and proclaimed it fine for EM use. After using the
microscope for a few weeks I noticed that I could never get a negative that
was in perfect focus. I collected negatives from several other users and
they all had the same problem. We contacted the engineers and one of them
spent the entire day trying to get perfect image of fresnel fringes. By
the time they turned of all the fans in our building (boy, did it get hot
and stinky), he was able to focus. It turned out a giant electrical cable
ran directly underneath the room to power the building ventilation. It
was, of course, not in use yet when they surveyed the new building! Not to
mention the nearby elevators that were not in use during survey time
either. Sadly for us, rather than spend $10,000.00 on an H frame to
eliminate the problem, they moved the old scope out and gave the space to a
molecular biology lab instead. Here we are 7 years later without a decent
EM facility in our building.




From daemon Thu Apr 13 00:38:29 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 12 Apr 2000 18:17:38 -0700
Subject: TP service

Contents Retrieved from Microscopy Listserver Archives
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Dear Bill,

The service is important, no question about that. But I agree, also, with
Tina, that it is not bad practice "do not disturb" working equipment. For
instance, our TP on JEM1200-EX TEM is original pump coming with instrument
15+ years ago. It was never serviced at all. Our JEOL service engineer
claimed that it is oldest working original TP on the JEM1200-EX series in
US. From time to time they called me asking does TP still working? And it
is work. I don't remember the exact model of TP. This is BALZERS for
sure. I think that electronics life depends in many cases not from how
long it operates but how many times you shut it ON and OFF. This kind of
"law" works perfectly for major electronic components as well as for CRTs,
computer components (HDs for instance). I guess, it may works for TPs too.
During start/stop TP components may sense some stress: more stress,
shorter life (we all know about that). I am experimented with magnetically
levitated TP from SEIKO (no financial interest) now. It works great. It
uses bearings only at low speed, at high speed the rotor is levitated.
Combination of this TP and "scroll pump" (oil free) gives me absolutely
oil-free vacuum system. I'll tell readers of this ListServer what happens
with that TP later. I am not going to service it at all.

Sergey


} Date: Wed, 12 Apr 2000 10:34:14 -0400
} From: William Tivol {tivol-at-wadsworth.org}
} Subject: Re: another turbo horror story
} Sender: tivol-at-wadsworth.org
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: Mozilla 4.07C-SGI [en] (X11; I; IRIX64 6.5 IP21)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Thu Apr 13 00:38:40 2000



From: earlw-at-pacbell.net
Date: Wed, 12 Apr 2000 19:55:51 -0700
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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I haven't been receiving the listserver emails for about one week.
Please re-subscribe.

Earl Weltmer



From daemon Thu Apr 13 00:38:50 2000



From: COFAB2-at-aol.com
Date: Thu, 13 Apr 2000 00:38:40 EDT
Subject: good ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As a new associate of Don G. of Microscopy Today I am interested located
interesting techniques and Problem/Solution type of information on all types
of microscopy.



From daemon Thu Apr 13 08:00:47 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Thu, 13 Apr 2000 13:53:48 +1000
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
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Dear Sergey!

In my dictionary I have found expression relevant to the situation: to
go from one extreme to another.
By my opinion to keep without service the mechanism rotated with
60,000 rpm is other extreme.
By analogy it is possible to refuse to change oil in car motors...?
Regards.

Victor Sidorenko, ANTRON Co.Ltd., scientific service, Moscow, Russia.



} ---------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}
}



From daemon Thu Apr 13 17:30:10 2000



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Thu, 13 Apr 2000 21:50:29 -0400
Subject: Coolwell Manuals & info

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} From: Hall, Ernest L (CRD)
Sent: Thursday, April 13, 2000 7:48 AM
To: 'MSA Listserver Dist'


Please correct me if I am wrong, but I think I learned from this list that
Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
with. No schematics and/or manuals to be found. Does anyone know where I
might find these or is willing copy important info from manuals they have on
this model? I would appreciate it.

Still in the fact finding phase but I suspect the thermostat is the main
trouble with this unit. Do any of you know a supplier for these? Thank you
in advance.

Joel McClintock
U of Kentucky



From daemon Thu Apr 13 17:30:16 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 13 Apr 2000 09:41:50 -0500 (CDT)
Subject: Re: good ideas

Contents Retrieved from Microscopy Listserver Archives
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Responding to the message of {c5.40b6c0e.2626a950-at-aol.com}
from "COFAB2-at-aol.com"-at-sparc5.microscopy.com:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} As a new associate of Don G. of Microscopy Today I am interested located
} interesting techniques and Problem/Solution type of information on all types
} of microscopy.
}
}


Whooooooooo are you.......who-oo....oo-oo. } :o

(With appologies to Pete Townsend)


Gib



Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Apr 13 17:30:18 2000



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Thu, 13 Apr 2000 09:34:59 -0600
Subject: SEM bacterial protocol

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I have a student that wants to look at Actinomycete colonies in the SEM.
I have not looked at bacterial COLONIES before. How would one prepare
such a sample?

TIA,
Bill Chissoe

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Thu Apr 13 17:30:19 2000



From: Mitch McCartney :      Mitch.McCartney-at-alconlabs.com
Date: Thu, 13 Apr 2000 10:57:38 -0500
Subject: Location of Position: Ft. Worth

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My apologies to everyone for not thoroughly reading the
advertisement. I posted the message for our Human Resources Department. I
didn't realize that Ft. Worth wasn't mentioned. Accordingly, to clarify
the advertisement, the position would be at our R&D facility in Fort Worth,
Texas, U.S.A.

Mitch
-------------------
Mitchell D. McCartney, Ph.D.
Associate Director
Central Sciences

Scientist
Retina Research - Degenerative Disease

Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle,
S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the
global leader in the discovery, development, manufacture and marketing of
ophthalmic pharmaceuticals and medical devices. Alcon expects to double its
sales over the next five years and has achieved a profit growth rate of
approximately 11% over the last several years. Historically, the company
commits 10% of sales to research and development. Products developed in the
last ten years generate 50% of current sales. The current product pipeline
is strong. Alcon was recently renamed to the Fortune List of the 100 Best
Companies to Work for in America.

As the successful candidate, you will conduct research in the area of
retinal degeneration aimed at identifying novel chemical agents to prevent
neurodegenerative disease using histopathologic and cell culture techniques.
You will participate in the design and provide expertise in preparation and
histopathologic evaluation of plastic and paraffin embedded retinal tissue,
including immunocytochemical techniques. You will also provide technical
expertise in cell/tissue based retina models; participate in handling,
manipulating, and dosing of various laboratory species; and interpret,
summarize, and communicate experimental results in appropriate formats.

Qualified candidates will have 1) at least nine years of applicable
experience, probably in a hospital or university laboratory, following
receipt of a B.S. in biology or a related discipline, or 2) at least three
years of applicable experience followed by receipt of an M.S. with thesis in
biology or a related discipline. Demonstrated competency in histology
techniques is required. Experience in cell/tissue culture is a plus.

Alcon professionals enjoy state-of-the-art facilities in a year-round
business casual environment. Our company offers competitive salaries and a
wide array of excellent benefits: a very generous retirement plan and
dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life,
and accident insurance, death, dismemberment, illness and disability
benefits, tuition reimbursement, employee credit union, adoption assistance,
dependent care, and wellness programs, on-site fitness center, running
track, cafeteria, and company store, innovative paid time off and holidays,
and retiree medical coverage.

An Equal Opportunity and Affirmative Action Employer. Pre-employment drug
testing.

Please forward your salary requirements and your resume to:

Job64_1261-at-careers.alconlabs.com
Reference Code: SRR





From daemon Thu Apr 13 17:30:19 2000



From: Laura Rhoads :      laura-at-lsrhoads.com
Date: Thu, 13 Apr 2000 12:00:18 -0400
Subject: vibration due to Mother Ship

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What about when the Mother Ship passes overhead?

Seriously, George has brought up an interesting point as I myself have
experienced the magnetic-interference-caused-by-powerline problem. The best
thing to do in all these cases is have the vendor (in my case John D from
JEOL) show up with the Earthquake Meter and Magnetic Treasure Finder and do
a survey before the decision-makers decide anything. It's even better if
you can work with the vendor BEFORE you let "them" plan anything.

This is one situation where asking forgiveness instead of permission
doesn't bode well...

"They" are watching...

Laura

}
} Hallo magnetic Microscopists !
}
} Everyone's been discussing vibration in this thread
} (talk about slavish adherence to topic ...) but there's
} another consideration - magnetic disturbances. Those
} truck have steel frames, so they'll disturb the Earth's
} magnetic field as they pass by. I inadvertently made a
} magnetometer once, and I could see the inflence of
} vehicles passing by fifty feet away on my oscilloscope
} screen. At M.I.T. there was a lot of concern about the
} nearby elevator in one EM lab ...
}
} Best regards,
} George Langford, Sc.D., who's got no vested interest in
} Earth's magnetic field except when out hiking.
} amenex-at-amenex.com
}



From daemon Thu Apr 13 17:30:21 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 13 Apr 2000 11:31:11 -0500
Subject: Coolwell Manuals & info

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Joel,

Here is all I know about Coolwell:

Coolwell Co. (Out of Business 4/99)
26 Law Drive, Fairfield, NJ 07004
973 882-6611, 800 367-5665
Bought out by: Lytron, Inc. (They have schematics)
55 Dragon Court, Woburn, MA 01801
Application engineer, Greg Ducharm, 781 933-7300
East coast sales, Scott Martin
Project engineer, Lonnie Fultz,4/00, Chillers
Independent- Frank Haze 800 367-5665
(SEE PECO MANUFACTURING FOR TEMP CONTROL)

PECO Manufacturing Co., Inc.
Portland, OR
Sunn(?) Division
503 233-6401
Tempurature control for Coolwell chillers
# TC103 025 C not available
OEM only. Made for Coolwell in lots of 100 only
(Coolwell list price was $170 each 1/99)

Switch used on temp control was made by: C & K (Unimax)
Original part #WHB152-9-W
C & K new part # HBS2KCB4SP011C (available from Newark Electronics #07F041)
22A, 125,277VAC, 15A 480VAC
1/4HP 125VAC, 1/2HP 250 270VAC

I have 3 Coolwell chillers (vintage 1993 and 1996), have replaced the
temperature control on 2 of them in the past year and am out of spare
controls. My units were over heating and shutting down. The temp control is
no longer available (unless all of us Coolwell owners pitch in and buy 100
of them). [I suspect that the sensing bulb is ok but that the electrical
switch has worn out. Test the bulb by immersing in hot water then cold
water while watching for expansion and contraction near the switch
actuator.] The switch could be replaced by drilling out it's securing
rivots and replacing (part # above). Use appropriate screws to replace
rivots as the switch must not slip. I have just ordered my new switches and
will be testing this fix soon.

Please let me know what you find. Coolwells were used on many SEMs so I
think we should have a few others interested in this problem. I have
schematics for our SE series units with various options and Lytron has been
very helpful.
Good Luck.
Jim


---------------------------

On 4/13/00 Joel McClintock wrote:

Please correct me if I am wrong, but I think I learned from this list that
Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
with. No schematics and/or manuals to be found. Does anyone know where I
might find these or is willing copy important info from manuals they have on
this model? I would appreciate it.

Still in the fact finding phase but I suspect the thermostat is the main
trouble with this unit. Do any of you know a supplier for these? Thank you
in advance.

Joel McClintock
U of Kentucky

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Thu Apr 13 17:30:27 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 13 Apr 2000 14:02:30 -0400
Subject: RE: Inexpensive Binoc OMs

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As I recall, a few days ago someone asked about a source of an inexpensive
abinocular optical microscope. Just yesterday I received an advertisment
from the Cole Parmer Co. (800-323-4340; www.coleparmer.com) for a 20X
binocular microscope for a base price of $159 (Cat. No.PP-03904-01), $220
when equipped with a top illuminator (PP-03904-02), and $240 equipped with
both top and bottom illuminators (PP-03904-03).

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Thu Apr 13 17:30:29 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 13 Apr 2000 15:57:07 -0400
Subject: RE: Tina's vac problem

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Tina:

It is possible that the reading you are getting from your vacuum gauge is
not as good as you expect because the gauge itself became contaminated with
oil. I presume the high vacuum gauge on your instrument is a Penning
gauge, as is the case for most EMs these days. If so, it might be useful
to take it off the instrument and clean it. We just had an incident where
a Penning gauge got so badly fouled that it would not fire at all, and thus
prevented the vacuum system from switching over to the high vacuum
evacuation mode, and so the vacuum could never become good enough to allow
the high voltage to be turned on.

It is usually not too difficult to clean a Penning gauge. The general
construction of these gauges is discussed in Sect. 3.2.2, p. 99, of my book
'Vacuum Methods in Electron Microscopy" and illustrated in Fig. 3.12 on
p. 101. Cleaning involves getting rid of the carbonaceous deposit that
forms on the inside wall of the gauge tube, and on the insulator that
supports the anode ring. Usually, depending on the design, these gauges
can be disassembled, somewhat as shown in Fig. 3.12, whereupon various
methods can be used to remove the carbonaceous deposits. One approach, of
course, is to use some kind of an abrasive (try Revere Ware Stainless Steel
Cleaner, or even a fine grade of metallographic polishing paper. Don't use
steel wool, because pieces of it will get attracted by the strong magnet
and become very troublesome to remove). The Hitachi service engineer was
successful in cleaning our gauge by boiling the gauge tube (after removing
the magnet from around it) and anode ring assembly in a STRONG detergent
solution (try Alconox, or a similar Lab. detergent) for 30 to 60 minutes,
periodically scrubbing with a toothbrush,

Good luck!

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Thu Apr 13 17:30:30 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Fri, 14 Apr 2000 16:24:31 -0500
Subject: Re: Coolwell Manuals & info

Contents Retrieved from Microscopy Listserver Archives
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on 4/13, Jim Romanov wrote:

} Please let me know what you find. Coolwells were used on many SEMs so I
} think we should have a few others interested in this problem. I have
} schematics for our SE series units with various options and Lytron has been
} very helpful.


We replaced the original thermostat on our Coolwell SE unit with an "ETC
Single Stage Electronic Temperature Controller" and a "1309007-044 ETC
Temperature Sensor" from Ranco (8115 U.S. 42 N., Plain City, Ohio, 43064).
The unit bolts right to the front of the Coolwell chiller and works just
fine. It works over a tempertature range of -30F to +220F and a
differential of 1F to 30F. I seem to recall that the whole shebang was
less than $100 and our campus refrigerator guy installed it with no
problems. I can fax the spec sheets to anyone who is interested. I
believe I got the idea from Maggy Piranian through this list.

Bob
Dr. Robert R. Wise
Associate Professor of Plant Physiology
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(920) 424-3404
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html



From daemon Thu Apr 13 18:36:18 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 13 Apr 2000 16:21:13 -0700
Subject: Re: Coolwell Manuals & info

Contents Retrieved from Microscopy Listserver Archives
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Dear Joel,
I have had good luck getting a local HVAC (heating, ventilation and air
conditioning) service firm to service my cooling water recirculators, which
are Haskris. They have repaired all of them without problems. They may be
able to fit replacement parts without having to resort to the manufacturer.
At 09:50 PM 4/13/00 -0400, you wrote:
}
} Please correct me if I am wrong, but I think I learned from this list that
} Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
} with. No schematics and/or manuals to be found. Does anyone know where I
} might find these or is willing copy important info from manuals they have on
} this model? I would appreciate it.
}
} Still in the fact finding phase but I suspect the thermostat is the main
} trouble with this unit. Do any of you know a supplier for these? Thank you
} in advance.
}
} Joel McClintock
} U of Kentucky

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Apr 13 21:32:31 2000



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Thu, 13 Apr 2000 18:33:22 -0500
Subject: Help with Forced Modulation Techniques

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Hi,
I was wondering whether anyone out there can help me out on the forced
modulation and phase contrast technoques. I'm familiar with the basics of
the AFM ,ie., contact and the non-contact modes. Now I want to learn the
advances methods. Can anyone suggest any good books that give the full
techniques?
Praveena

Praveena M Bhaskara
MS Student
Chemical Engineering Department
University of Massachusetts, Lowell
Lowell, MA 01854
Email:bubbyp-at-hotmail.com
PH:978-459-0175
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com




From daemon Thu Apr 13 21:32:31 2000



From: deweese-at-fas.harvard.edu () (by way of Nestor J. Zaluzec)
Date: Thu, 13 Apr 2000 18:37:29 -0500
Subject: Re: ? mitochondria. With 400 times magnification

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Email: deweese-at-fas.harvard.edu
Name: Alex de Weese
School: Harvard College
Question: Hello,
I just finished a lab where we did cell fractionation, and looked at
nuclear and cytosolic fractions stained with toluidine blue under a zeiss
compound light microscope. I was wondering why I couldn't see
mitochondria. With 400 times magnification, I expected to be able to see
them in the cytosolic fraction. Is it that the mitochondria are not
staining? Is it a problem with contrast?
Thanks for your help.
Sincerely,
Alex de Weese


---------------------------------------------------------------------------




From daemon Thu Apr 13 21:32:32 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 13 Apr 00 17:53:44 -0700
Subject: Re: good ideas

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Reply to: Re: good ideas
Well put.

Messages with only a name, or even less, I just delete now.

It is so easy to sign these things, and so interesting to know who is writing them.

Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Gib Ahlstrand wrote:
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} Responding to the message of {c5.40b6c0e.2626a950-at-aol.com}
} from "COFAB2-at-aol.com"-at-sparc5.microscopy.com:
} } } } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } -----------------------------------------------------------------------.
} } } } } } As a new associate of Don G. of Microscopy Today I am interested located } } interesting techniques and Problem/Solution type of information on all types } } of microscopy.
} } } } }
}
} Whooooooooo are you.......who-oo....oo-oo. } :o
}
} (With appologies to Pete Townsend)
}
}
} Gib
}
}
}
} Gib Ahlstrand
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
} http://biosci.umn.edu/MIC/consortium.html
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}
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Apr 13 21:32:33 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 13 Apr 00 17:59:48 -0700
Subject: RE: Coolwell Manuals & info

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Reply to: RE: Coolwell Manuals & info
Yes Joel,

Frank Haze, who owned Coolwell (and what magnificent chillers they are) sold the company to Litron (phone: 781 933 7300). I do not know to what extent they will continue to support the chillers but the contact I was given at Litron was Lohny Fultz.

Our chillers are currently being maintained by our refrigeration service contractors and they appear to be doing a great job of it (Cascade Refrigeration, Irvine CA).

Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Joel McClintock wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Please correct me if I am wrong, but I think I learned from this list that
} Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
} with. No schematics and/or manuals to be found. Does anyone know where I
} might find these or is willing copy important info from manuals they have on
} this model? I would appreciate it. }
} Still in the fact finding phase but I suspect the thermostat is the main
} trouble with this unit. Do any of you know a supplier for these? Thank you
} in advance. }
} Joel McClintock
} U of Kentucky
}
}
}
}
} RFC822 header
} -----------------------------------
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From daemon Fri Apr 14 07:24:34 2000



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Fri, 14 Apr 2000 08:58:21 +0200
Subject: Durst condenser lenses - cleaning

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Hello,
We have in our lab a Durst Laborator 138S enlarger. After all those
years of it's perfect working, there are some layers of dust on
condenser lenses. Does anybody know, how to safely clean the surface
of condenser lens? Thanking you in advance.
O. Benada

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743



From daemon Fri Apr 14 18:25:30 2000



From: ComCryLab1-at-aol.com
Date: Fri, 14 Apr 2000 09:20:02 EDT
Subject: LM: Inexpensive Binoc OMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Prof. Bigelow,
Thanks for that info. I'll check it out.
Mike Urbanik
www.crystalguru.com



From daemon Fri Apr 14 18:25:31 2000



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Fri, 14 Apr 2000 09:34:42 -0400
Subject: Help with Forced Modulation Techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Praveena: One place to start is Digital Instruments web site--DI.com.
They have many applications notes on the subjects you are interested in. I
believe other manufacturers also have helpful web sites. Steve



Hi,I was wondering whether anyone out there can help me out on the forced
modulation and phase contrast technoques. I'm familiar with the basics of
the AFM ,ie., contact and the non-contact modes. Now I want to learn the
advances methods. Can anyone suggest any good books that give the full
techniques?
Praveena





Stephen McCartney
Research Associate
Virginia Tech
Materials Institute
2108 Hahn Hall
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Fri Apr 14 18:25:31 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 14 Apr 2000 07:46:12 -0700
Subject: Re: Durst condenser lenses - cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If it like most condenser enlargers, the condenser lenses are
opposing each other and retained in a metal cylinder. Just
remove the cylinder, lenses and clean them with a cloth towel,
moistened in a solution of Simple Green or a store-bought
lens cleaning solution. rinse the lenses, wipe them dry and
use a duster to get all moisture and lint off of them. That ought
to do it. Check your enlarger's lens while you are at it.
And also see if the bellow and lens plate areas need blowing out.
Lots of dust collects in those nooks and crannies.

gary g.


At 11:58 PM 4/13/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Apr 14 18:25:32 2000



From: Chris Walker :      chris.walker-at-physics.org
Date: Fri, 14 Apr 2000 15:37:47 +0100
Subject: EM - Magnetic field strength in SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me the typical magnetic field strength at the sample in an
SEM and (if possible) how fast the field drops off with distance from the
axis?. Any references would be gratefully received.

Regards
Chris Walker



From daemon Fri Apr 14 18:25:32 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 14 Apr 2000 10:59:20 -0400
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey Ryazantsev wrote:

Dear Sergey,


} I think that electronics life depends in many cases not from how
} long it operates but how many times you shut it ON and OFF.

I agree that this is true, especially with computers and, in this case,
pump controllers. BTW, we have had to fix the controllers on several
occasions.


} This kind of
} "law" works perfectly for major electronic components as well as for CRTs,
} computer components (HDs for instance). I guess, it may works for TPs too.

Maybe not. There are two competing factors: There is wear on the bear-
ings due to the inevitable friction of running. Changes due to acceleration
of the pump during shutdown and startup cause an increase in wear. To get
optimal performance, these factors must be balanced. Our pumps have
mechanical bearings, which will wear during the normal operation of the
pump, so we have chosen to get the bearings replaced every two years.
Since we are replacing the part that wears, the pump lifetime should not
be shortened by this procedure. We also change the bearing oil on a regular
schedule; I think that this prevents any problems from oil breakdown and/or
acidification. There is negligable bearing wear produced by the oil changes,
and the bearing replacement will prevent the accumulation of even this wear.

}
} During start/stop TP components may sense some stress: more stress,
} shorter life (we all know about that). I am experimented with magnetically
} levitated TP from SEIKO (no financial interest) now. It works great. It
} uses bearings only at low speed, at high speed the rotor is levitated.

With this kind of pump stop/start cycles will cause the mechanical
bearings to be used, so there is logic in leaving them running continually.


}
} Combination of this TP and "scroll pump" (oil free) gives me absolutely
} oil-free vacuum system. I'll tell readers of this ListServer what happens
} with that TP later. I am not going to service it at all.

Sounds like a good system; I'm glad you plan to post how things go.
Yours,
Bill Tivol



From daemon Fri Apr 14 18:25:33 2000



From: HOWARD L. MULHERN :      mulhern-at-hub.tch.harvard.edu
Date: Fri, 14 Apr 2000 11:50:54 -0400
Subject: TEM RESOLUTION

Contents Retrieved from Microscopy Listserver Archives
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I have a question concerning TEM Resolution.

We are currently in the process of replacing our aging Phillips
300 TEM with a brand new all digital instrument that is going to be
installed in virtually the same position as the old scope. As
supervisor of the surgical pathology EM facility I have to say we have
never had to question the resolution of the 300 and given the often sub
optimal specimens that we work with the photo's are crisp and sharp. Any
out of focus photographs are usually the result of out of focus
operators, the occasional helicopter landing on the roof and yes trucks.
This is seldom an issue and certainly not the scope.
Here's our problem. We just had a site visit to measure the
electrical and magnetic forces in the room (standard for installation of
any new microscope). And to my suprise the room has an
electrical/magnetic problem that "May effect the resolution of the new
instrument" and quite possibility "the new scope will not meet spec in
this location". So what to do. I seriously doubt that the old scope is
giving me better resolution than the new scope will and I can't change
locations at this point.
So for biology or really anyone embedding in epoxy resins is
there a standard or a number in angstroms that we can say is the minimum
we will accept. If I am pleased with my photo's taken at 60,000 X do
really need to insist that the instrument be capable of taking a sharp
photo at 300,000X.

Howard Mulhern



From daemon Fri Apr 14 18:25:34 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Fri, 14 Apr 2000 10:11:31 -0600
Subject: SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David, Martyn,

this problem is not limited to image processing, of course. IP only
makes it easier to use or abuse these things. If you think about how
you get the signals from the SEM (or any other image source), there are
many ways the signal can be distorted. For example, the detector may not
be linear (as is the case for many old cameras and, for that matter, the
human eye), signals may be distorted during transmission, noise is
added, the film for photographing has certain characteristics, and the
darkroom work can be more of an art than science.
Forensic science has had to deal with this for some time. If an argument
in a court of law can be made that a picture has been "doctored", it
will lose it's effectiveness or be dismissed. In Forensics it is very
important to keep a record of the whereabouts and the processing done to
"evidence" at all times. Sometimes people burn a "gray scale" into the
image before they do any processing. This gray scale will then show what
happened to the image during processing. Of course, this is not
foolproof as anybody can put on another gray scale after processing. But
this is the same as inventing measurement data, which can be done, but
is not a real problem in science as it just cannot be repeated
independently.
If you need to be absolutely sure, get a step-by-step recipe of the
image processing and have someone else repeat it. If the results are
different, you have reason to be concerned. If they are identical, you
have your answer.
You need to be familiar with the possibilities and shortfalls of image
processing. You can start with an image that shows just noise, apply
some Fourier-filters and other processing and end up with a periodic
structure on the image. This, however, is not due to the image
processing, it is due to using the wrong tools (or use the tools the
wrong way).

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: David Henriks [mailto:Henriks-at-CompuServe.COM]
Sent: Wednesday, April 12, 2000 11:14 AM
To: Martyn Harris; Micro Listserver



Dear Martyn:

Several years ago (MSA in Cleveland if I recall correctly), there was
some
sort of roundtable discussing the ethics of image manipulation. I can't
remember much more than that, but perhaps those with a better memory who
may have participated in the discussion would have some good input.

Best regards-

David
Writing at 9:53:20 AM on 04/12/2000

************************************************************************
***
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX:
+1-949-492-1499
San Clemente, CA 92673 USA e-mail:
henriks-at-southbaytech.com

************************************************************************
***
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by
INTERNET:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
} A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and
staining
methods are used to obtain and or enhance selected features prior
to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing
capabilities

of image processing at my fingertips it's possible to ' modify' and

possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of
different

sem capabilities / preparation methods / etches or are they simply

'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to
enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot
believe
what you see ?

Regards.

{



From daemon Fri Apr 14 18:25:34 2000



From: lherault :      lherault-at-bu.edu
Date: Fri, 14 Apr 2000 00:34:39 -0400
Subject: polyacrylamide gel for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A student has come to me with the following problem. Can anyone assist us?

Ron L.

Please cc answers to:

saeeda-at-bu.edu


Does anybody know of any good fixing/dehydrating techniques for viewing this
sample using the SEM? Alcohol and CPD dehydration techniques are not
yielding good results and there is a lot of distortion of the gel. I am
looking for something that will enable me to preserve the features of the
gels, and analyze their morphology. These gels will be patterned with
collagen and cells will be grown on them. I am interested in viewing these
patterns of collagen, and the corresponding patterns of cell growth.
Thanks.

Saeeda



From daemon Fri Apr 14 18:25:35 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Fri, 14 Apr 2000 13:09:45 -0400
Subject: Durst condenser lenses - cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If the lens is coated, which some Durst condensers are, I would suggest
starting with
a Blower Brush, available in most camera shops. Hopefully you can
blow/brush most or all
the dust away. If that fails, one of the new "microfiber" lens cloths work
very well
on most lenses. A high quality photographic lens cleaning solution should
be safe,
but I would try to clean without any chemicals first.

George Laing
National Graphic Supply
Albany, NY USA
www.ngscorp.com


-----Original Message-----
} From: Oldrich Benada [mailto:benada-at-biomed.cas.cz]
Sent: Friday, April 14, 2000 2:58 AM
To: Microscopy-at-sparc5.microscopy.com


Hello,
We have in our lab a Durst Laborator 138S enlarger. After all those
years of it's perfect working, there are some layers of dust on
condenser lenses. Does anybody know, how to safely clean the surface
of condenser lens? Thanking you in advance.
O. Benada

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743




From daemon Fri Apr 14 18:25:35 2000



From: Norman Michaud :      Norman_Michaud-at-meei.harvard.edu
Date: Fri, 14 Apr 2000 13:43:14 -0400
Subject: TEM- used microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a supplier of used ultramicrotomes? I'm looking for a
Leica ultracut E, in good working order. Is there anyone who is dismantling
a TEM lab who wants to sell one?
Thanks.

Norman Michaud
Director, Morphology
Mass Eye and Ear Infirmary
Ophthalmology-5th flr.
243 Charles St, Boston, MA 02114
norman_michaud-at-MEEI.Harvard.edu
Tel:617-573-3316; Fax:617-573-4290



From daemon Fri Apr 14 18:25:36 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 14 Apr 2000 13:51:15 -0500
Subject: Re: polyacrylamide gel for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can suggest two possibilities:1) atomic force microscopy of the
hydrated gel; 2) high-pressure freezing of the gel, followed by
either cryoSEM or cryo-ultramicrotomy and cryoTEM (cryoSEM would be
simpler than cryo-ultramicrotomy).

Phil

} A student has come to me with the following problem. Can anyone assist us?
}
} Ron L.
}
} Please cc answers to:
}
} saeeda-at-bu.edu
}
}
} Does anybody know of any good fixing/dehydrating techniques for viewing this
} sample using the SEM? Alcohol and CPD dehydration techniques are not
} yielding good results and there is a lot of distortion of the gel. I am
} looking for something that will enable me to preserve the features of the
} gels, and analyze their morphology. These gels will be patterned with
} collagen and cells will be grown on them. I am interested in viewing these
} patterns of collagen, and the corresponding patterns of cell growth.
} Thanks.
}
} Saeeda

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Fri Apr 14 18:25:38 2000



From: Susanne S¿rensen :      sus.sus-at-danbbs.dk
Date: Fri, 14 Apr 2000 21:52:34 +0200
Subject: TEM "Cryosections, lipid"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello world
I think i have tried everything, but still!!
Is there somebody, who nows how to detect Glycolipid/lipid on cryo ultrathin sections?
And yes, i have read Dr. Wim's article, where he compare different metodes.
I have been on a course in Austria, where they also told me, that this ( Dr. Wim) would be the way to decet lipid.
But in the real world, it does not work. The lipid is floating all over the sections.
Tanks for your help
Sincerely
Sus S¿rensen



From daemon Fri Apr 14 19:02:58 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 14 Apr 2000 16:07:08 -0700
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bill,
It was nice discussion there. Thanks for your nice reply. I totally agree
with you. No question, the good service in time is a great deal!
Unfortunately the service sometimes is not such great as you have with
yours TPs. Again, sometimes people just do not have enough money to do
service in time.

As for magnetically levitated TP, you right, it is good idea to keep it
running continuously. Currently, I am working on my system in the way to
be able to insert samples through air-lock, than it will be possible do not
break vacuum to load samples. This is my plan. Wish me luck.
Sergey

} Date: Fri, 14 Apr 2000 10:59:20 -0400
} From: William Tivol {tivol-at-wadsworth.org}
} Subject: Re: TP service
} Sender: tivol-at-wadsworth.org
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: Mozilla 4.07C-SGI [en] (X11; I; IRIX64 6.5 IP21)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri Apr 14 19:03:01 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 14 Apr 2000 15:34:34 -0700
Subject: Re: LM: Inexpensive Binoc OMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike -

There are a LOT of suppliers of inexpensive light microscopes in the
educational marketplace; many of the scopes are remarkably good. You'll
find a list of suppliers on the Project MICRO web page (URL below). Get
several catalogs; if you compare descriptions and photos, you'll realize
that prices can vary almost 50% on the same item.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Fri Apr 14 21:17:47 2000



From: catchanangel-at-aol.com
Date: Fri, 14 Apr 2000 21:10:58 -0500
Subject: Question: photobleaching vs quenching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Colleagues

Can anyone explain this to Angela. It's not my field.
She is not on the Listserver.

Nestor
Your Friendly Neighborhood SysOp


Email: catchanangel-at-aol.com
Name: Angela Lowry
School: Deer Valley High School

Question: What is the difference between photobleaching and quenching in
two-photon laser scanning microscopy?

---------------------------------------------------------------------------




From daemon Fri Apr 14 21:49:45 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, April 14, 2000 3:11 PM
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Bill Tivol. It is less stressfull on electrical circuits and
contacts to leave the power on. To do so prolongs the life of the part, as
well as the reliability of voltage flow through an instrument. A good
example is XRD and XRF tubes. Rule #1 I learned as a grad student was to
always leave the power on, keep a current flowing through the tubes. Why?
A power current "burns in" at a single point on the tube. If the power is
shut off, the burn in point can drift. Drift actually weakens the tube,
which is the opposite of what you would expect. The drift will also cause
current fluctuations, creating another source of error reducing the
precision and accuracy of the measurements. At $3000-$4000 per pop on a
tube, it is prudent to do what ever you can to extend its life. Same applies
to any electrical instrumentation or accessories.

Lou Solebello
-----Original Message-----
} From: William Tivol {tivol-at-wadsworth.org}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Fri Apr 14 22:51:25 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 15 Apr 2000 20:31:36 +0100
Subject: Re: TEM RESOLUTION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: Michele Palmer {moonlite-at-csd.uwm.edu}
To: {wchiss-at-ou.edu}
Sent: Friday, April 14, 2000 7:53 PM


Hi

The question of much resolution do we really need and what resolution is
obtainable often confronts the EM service engineer and the EM consultant?

As mentioned before we at Protrain buy instruments for clients or in
conjunction with clients. On many occasions the proposed site does not meet
the manufacturers requirements in their entirety but circumstances mean we
must go ahead.

Whilst these situations are not ideal one must consider the clients
application and the desired performance for that application. On many
occasions checking an instrument in a clients laboratory, where the client
does not see a problem in their results (5,000 to 30,000X), we see image
instability at 200,000X. As a service engineer you judge the problem and
decide if it is a simple fix or a cause for deep investigation. Often
discussions with the client result in keeping going whilst constantly
checking the magnitude of the fault. This route may be followed for many
years before the engineer, as the client still has no visible micrograph
problems, decides to get in and chase the fault.

So what am I saying? In the 70s high quality work was carried out on TEM
that had a point to point resolution of around 1nm. We are all aware that
1nm at 100,000X is equal to 0.1mm on the micrograph and we need a hand lens
to visualise this! In most routine medical observations, other than virus
work, the typical max is about 30,000X, about 3nm limited by
the section thickness (?) so would it not be sufficient to use a 1nm
machine?

Many biologists still use very low kV, i.e. 80, so they are degrading their
0.3nm instrument still further. Running at 100 or 120kV will provide better
quality images
using dark room procedures to aid contrast. Higher accelerating voltage
will help
with fields and the modern antivibration systems, available as discussed
earlier this month, will help overcome floor vibration.

So my advice after working with TEM for 36 years all over the world in all
sorts of very poor environments is GO FOR IT! I will not take up space by
relating the amazing stories of dreadful sites and superb microscopy, it
certainly does happen!

Good luck

Steve Chapman
Senior Consultant
Protrain - for consultancy and training world wide
Tel & Fax +44 01280 814 774
e-mail protrain-at-emcourses.com
web www.emcourses.com




From daemon Sun Apr 16 16:44:45 2000



From: Steve Beck :      becks-at-sunynassau.edu
Date: Sun, 16 Apr 2000 12:51:15 -0400
Subject: Summer 2000 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SUMMER I 2000 COURSE ANNOUNCEMENT - Transmission Electron Microscopy
(BIO. 221-Section B)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A five week, Summer Session I 2000 semester, course in Biological
Transmission Electron Microscopy is being offered by the Biology Department
of Nassau Community College. This is a 4 credit course offered four days
per week (Monday through Thursday) between the hours of 8:00 am and NOON.
Classes will begin on May 22 and end on June 22, 2000.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$86 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM
photomicrographs is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

For information about mail or telephone registration (Dial-a-Course) point
your browser to http://www.sunynassau.edu/courses/Sum00/index.html.

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Sun Apr 16 16:44:45 2000



From: Steve Beck :      becks-at-sunynassau.edu
Date: Sun, 16 Apr 2000 12:50:22 -0400
Subject: Summer 2000 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SUMMER I 2000 COURSE ANNOUNCEMENT - Transmission Electron Microscopy
(BIO. 221-Section B)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A five week, Summer Session I 2000 semester, course in Biological
Transmission Electron Microscopy is being offered by the Biology Department
of Nassau Community College. This is a 4 credit course offered four days
per week (Monday through Thursday) between the hours of 8:00 am and NOON.
Classes will begin on May 22 and end on June 22, 2000.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$86 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM
photomicrographs is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

For information about mail or telephone registration (Dial-a-Course) point
your browser to http://www.sunynassau.edu/courses/Sum00/index.html.

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Sun Apr 16 18:54:34 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 16 Apr 2000 16:41:21 -0700
Subject: Olympus BX DIC and phase LM systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have two new condition Olympus LM systems for sale.

Transmitted DIC:

BX60 stand
trinoc head
2ea 10X eyepieces
6-place DIC nosepiece
DIC slider
DIC analyzer
Universal condenser
Rt stage
DIC inserts for 20, 40, 100X
UPlan FL 4X, 10X, 20X, 40X, 100X objectives
100 Watt lamphouse

Like new condition throughout.


Transmitted phase contrast:

BX50 stand
trinoc head
2ea 10X eyepieces
6-place nosepiece
Phase condenser
Phase inserts
Rt stage
UPlan FL Phase 4X, 20X, 40X, 60X, 100X, Plan 10X objectives
100 Watt lamphouse

Like new condition throughout.

Please contact me if you are interested in either of
these system. Telecon is 916.791.8191

I also have two Olympus PM10AD computer control photo
systems which include the control unit, shutter body,
focusing telescope and connecting cable. PE eyepieces
are also available.

tnx,
gary g.



From daemon Sun Apr 16 22:44:01 2000



From: arthurmott-at-netzero.net ()
Date: Sun, 16 Apr 2000 22:36:27 -0500
Subject: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone answer this?

Nestor

--------------------------------------------------------------------------

Email: arthurmott-at-netzero.net
Name: Arthur Mott

Question: I have a Bausch & Lomb 0.7X-3X which I wish to convert to
gemological use(viewing gem stones). What type of lighting systems should
I use , or where can I gather additional information.

Arthur

---------------------------------------------------------------------------




From daemon Mon Apr 17 08:06:01 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 17 Apr 2000 16:49:00 GMT+1200
Subject: Off and On

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Lou

}
}
} I agree with Bill Tivol. It is less stressfull on electrical circuits and
} contacts to leave the power on. To do so prolongs the life of the part, as
} well as the reliability of voltage flow through an instrument. A good
} example is XRD and XRF tubes. Rule #1 I learned as a grad student was to
} always leave the power on, keep a current flowing through the tubes. Why?
}
} A power current "burns in" at a single point on the tube. If the power is
} shut off, the burn in point can drift. Drift actually weakens the tube,
} which is the opposite of what you would expect. The drift will also cause
} current fluctuations, creating another source of error reducing the
} precision and accuracy of the measurements.
}
}

This is a pretty interesting way of looking at things, but it seems
like an argument for leaving things eg X-Ray tubes running at their
working conditions (kV and mA) fulltime.
Can you expand on this "single point"?
Is it some physical point on, for example, a filament, or is it kind
of metaphorical?
I can't quite understand the mechanism of the phenomenon you are
describing.
I've always just thought that the reason for leaving X-Ray tubes on,
but at minimum power, was to avoid the current inrush through a
cold (and therefore low-resistance) filament, plus the thermal
stresses in repeatedly warming up and cooling down of the tube and
all its glass-to-metal seals..

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Apr 17 08:06:02 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 17 Apr 2000 02:02:06 EDT
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi there Arthur,
it is a dark field method of illumination and gemological assn. of America
sells a base that the b and l pod will fit. there are also some third parties
that do this base also but any of them can be pricey.
check out gia on the net. also try search under key gemscope
thanks Ed Sharpe archivist for SMECC



From daemon Mon Apr 17 08:06:07 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Mon, 17 Apr 2000 12:47:23 +0200
Subject: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

Whilst working in Australia we found a few labs using something called
Shellite as a de-greaser to clean microscope parts. This works very well and
seems to be very available. we then looked for the same product here in SA.
We then found that this is a trade name only in Australia and is actually
petroleum ether or spirit.
We called the local Shell distributor to ask what the difference was between
spirit and ether. Well, they were not sure on that one. Then we were told
you must specify the temperature range of the petroleum Ether. 40 - 60, 60 -
80 or 80 - 100 deg Celsius. Again no explanation as to what the difference
is an why.
Can any one give us some more info on Petroleum ether / spirit and the
advantages disadvantages on the different temperatures.


Thanks

Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
Fax + 27 (0) 11 476 7290
anaspec-at-icon.co.za
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com



From daemon Mon Apr 17 08:06:10 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Monday, April 17, 2000 4:56 AM
Subject: Off and On

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is a real physical phenomonom. I dont think I understand it correctly
myself, except if I think about like a spark plug in a car. You are right
about the vacuum, but take a look at some of your old tubes if you have any.
You should be able to see a gray smoky discoloration of the glass tubing at
some area on the tube, usually on one side near the center. That is the burn
in point.

That also brings up a curiosity question to me....Does it happen to the
newer cermaic tubes? I dont know, and will have to ask. The newer ceramic
tubes are supposed to have a longer life and reliability compared to the
glass ones.
-----Original Message-----
} From: Ritchie Sims {r.sims-at-auckland.ac.nz}
To: Lou Solebello {microls1297-at-mindspring.com} ;
Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}


Hi, Lou

}
}
} I agree with Bill Tivol. It is less stressfull on electrical circuits and
} contacts to leave the power on. To do so prolongs the life of the part, as
} well as the reliability of voltage flow through an instrument. A good
} example is XRD and XRF tubes. Rule #1 I learned as a grad student was to
} always leave the power on, keep a current flowing through the tubes. Why?
}
} A power current "burns in" at a single point on the tube. If the power is
} shut off, the burn in point can drift. Drift actually weakens the tube,
} which is the opposite of what you would expect. The drift will also cause
} current fluctuations, creating another source of error reducing the
} precision and accuracy of the measurements.
}
}

This is a pretty interesting way of looking at things, but it seems
like an argument for leaving things eg X-Ray tubes running at their
working conditions (kV and mA) fulltime.
Can you expand on this "single point"?
Is it some physical point on, for example, a filament, or is it kind
of metaphorical?
I can't quite understand the mechanism of the phenomenon you are
describing.
I've always just thought that the reason for leaving X-Ray tubes on,
but at minimum power, was to avoid the current inrush through a
cold (and therefore low-resistance) filament, plus the thermal
stresses in repeatedly warming up and cooling down of the tube and
all its glass-to-metal seals..

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand




From daemon Mon Apr 17 08:06:10 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Mon, 17 Apr 2000 14:24:50 +0100 (BST)
Subject: petroleum ether

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The difference between photobleaching and quenching is quite fundamental.

Basically when a molecule is photobleached it is converted to another
molecule that is not fluorescent -i.e. a reaction takes place in which the
fluorescing species is converted to another species.

Quenching on the other hand results from the excited fluorescent molecules
having a non radiative route to release the energy it would typically
release as fluorescence. The state of the molecule after the quenching
however is the same as if it had fluoresced.
----------------------------------------------------------------------------
---------------------------
Dr. Giles Sanders
Zeneca / SmithKline Beecham Centre for Analytical Sciences
Chemistry Department
Imperial College of Science, Technology and Medicine
London
SW7 2AY

(44) - 0171-594-5749



Never express yourself more clearly than you think.
-- Niels Bohr (1885-1962) Danish physicist

----------------------------------------------------------------------------
------------------------
----- Original Message -----
} From: {"catchanangel-at-aol.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: 15 April 2000 03:10


Luc,
The terms "petroleum spirit" and "petroleum ether" are
interchangeable. You may also encounter "light petroleum"
and "pet ether". The numbers correspond to the boiling
ranges in degrees celsius - they are just differnt
fractional distillates. The 40-60 fraction is
roughly hexane ( plus small amounts of larger and smaller
hydrocarbon molecules). The higher boiling fraction will
not evaporate as cleanly as the lighter ones, but all are
good solvents for grease and oil. They are, of course,
highly flammable.

Regards,
Eric

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk





From daemon Mon Apr 17 11:36:06 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 17 Apr 2000 08:35:11 -0500
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Check the web site for the Gemmological Institute of America (GIA). I
forget the exact URL, it's at home (www.gia.com? .org?). They sell a
stereoscope designed for exactly this purpose, and looking at it will
give you a good idea of what you need.

Briefly, it uses separate transmitted and reflected light sources.
The transmitted light I believe is an "ordinary" tungsten microscope
bulb, and the reflected source is a closely mounted fluorescent
source. The stones are held by a "third-hand" type of gripper so that
it can be examined from all angles. This is important not just in
studying inclusions, but because the optical characteristics
(including color) of some gems change with the crystal axis (the
Usambara effect, if I've spelled that right). Different types of
light are also needed, as color-change gems show different colors
depending on the incident light. For best effect, I would also had an
optical fiber source with dual goosenecks (not a ring-light, except
in addition), and a *good* mirror to shine sunlight on the specimen.
This is for judging stone color as well as color change.

The GIA site is a good source of information, but I'd check the Nat.
Mus. Natural Hist./Smithsonian, and the Mus. Nat. Hist. in London
also, to start.

Phil

} Can anyone answer this?
}
} Nestor
}
} --------------------------------------------------------------------------
}
} Email: arthurmott-at-netzero.net
} Name: Arthur Mott
}
} Question: I have a Bausch & Lomb 0.7X-3X which I wish to convert to
} gemological use(viewing gem stones). What type of lighting systems should
} I use , or where can I gather additional information.
}
} Arthur
}
} ---------------------------------------------------------------------------

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Mon Apr 17 11:36:06 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 17 Apr 2000 08:37:53 -0500
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Naturally I forgot the darkfield. (#*&$(&# Probably because it's important.

Phil

} hi there Arthur,
} it is a dark field method of illumination and gemological assn. of America
} sells a base that the b and l pod will fit. there are also some third parties
} that do this base also but any of them can be pricey.
} check out gia on the net. also try search under key gemscope
} thanks Ed Sharpe archivist for SMECC



From daemon Mon Apr 17 11:36:15 2000



From: Dr. Hodi Klara :      hodi-at-pharma.szote.u-szeged.hu
Date: Mon, 17 Apr 2000 17:49:00 MET
Subject: e-mail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear colleagues,

some days I have any e-mail massage, perhaps my address was delated.
Write, please, my address again.

Best regards,

Dr. Klara Pintye-Hodi



From daemon Mon Apr 17 11:36:16 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 17 Apr 2000 08:43:49 -0700
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

What do you mean by "convert"? Are you referring to a "dissecting" scope?
If so, you multiply the number that you've given (presumabvly the power of
the objective lens) times the power of the eyepiece, which is often 10x.
That would mean that you may have 30x available, and that definitely
requires good illumination. My son-in-law, who is a professional diamond
cutter, really likes the flexibility and brightness of fiber optic
illuminators - but they're expensive. The Edmund Optical catalog is a good
place to begin. If you want specific gemological advice, contact the
Gemological Institute of America (GIA).

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Mon Apr 17 18:35:32 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Mon, 17 Apr 2000 10:33:29 -0600
Subject: EM - Magnetic field strength in SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

I don't think, there is an easy answer to your question. It depends on
the microscope type (immersion lens or not), the lens configuration and
design, and last but not least on the working distance, aside from the
lens current, which depends on the acceleration voltage.

I have a few pointers for you, though. I remember faintly a book by
Glaser, but that may be in German (anybody with a good reference for
this book?).

Another book is: Ludwig Reimer (Scanning Electron Microscopy : Physics
of Image Formation and Microanalysis (2nd Ed)(Springer Series in Optical
Sciences, Vol 45) )

Here is a URL for the book at Amazon.com:
http://www.amazon.com/exec/obidos/ASIN/3540639764/qid=955982926/sr=1-17/
102-8036949-3232817

This book is a bit "theoretical", but has information about electron
optics in the first two chapters.

You may want to search the net for "electron optics".

Hope this helps.

Michael

(disclaimer: I have no interest in Amazon.com. Check out other
bookstores for prices)

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Chris Walker [mailto:chris.walker-at-physics.org]
Sent: Friday, April 14, 2000 8:38 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Can anyone tell me the typical magnetic field strength at the sample in
an
SEM and (if possible) how fast the field drops off with distance from
the
axis?. Any references would be gratefully received.

Regards
Chris Walker



From daemon Mon Apr 17 18:35:39 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 17 Apr 2000 16:02:29 EDT
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The pod, i.e., the top part that is the business end just comes out of the
stand of a b&l zoom and drops in the gia base...

Of course for adventure one can build the illuminating base necessary by
hand if so inclined.

Ed Sharpe

{ { Subj: Re: Bausch & Lomb Question:
Date: 4/17/00 11:41:32 AM US Mountain Standard Time
From: schooley-at-mcn.org (Caroline Schooley)
To: arthurmott-at-netzero.net
CC: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
}
} Email: arthurmott-at-netzero.net
} Name: Arthur Mott
}
} Question: I have a Bausch & Lomb 0.7X-3X which I wish to convert to
} gemological use(viewing gem stones). What type of lighting systems should
} I use , or where can I gather additional information.
}
} Arthur -

What do you mean by "convert"? Are you referring to a "dissecting" scope?
If so, you multiply the number that you've given (presumabvly the power of
the objective lens) times the power of the eyepiece, which is often 10x.
That would mean that you may have 30x available, and that definitely
requires good illumination. My son-in-law, who is a professional diamond
cutter, really likes the flexibility and brightness of fiber optic
illuminators - but they're expensive. The Edmund Optical catalog is a good
place to begin. If you want specific gemological advice, contact the
Gemological Institute of America (GIA).

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microsc } }



From daemon Mon Apr 17 18:35:44 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Mon, 17 Apr 2000 14:37:48 -0700 (Pacific Daylight Time)
Subject: ISI (Topcon) Service contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am in need of a service contract for an ISI DS-130 SEM.
Is there anyone who is particularly happy with a contract
on their ISI (Topcon) instrument?
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Mon Apr 17 18:35:47 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 17 Apr 2000 18:25:16 -0400
Subject: RE: EELS: need spectrum for comparing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try the following EELS database site. The have a number of O containing
materials there with all of the acquisition parameters well documented.
http://www.cemes.fr/eelsdb/

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Jo Verbeeck [mailto:joverbee-at-ruca.ua.ac.be]
} Sent: Tuesday, April 11, 2000 10:09 AM
} To: Microscopy listserver message adress
} Subject: EELS: need spectrum for comparing
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello,
}
} I`m looking for an EELS spectrum containing Oxygen K-edge and some
} other edge like Mn or Ti L-edge. Together with low loss
} spectrum and data
} about the angles.
} The spectra need to have a power of 2 (1024 for inst.) bins
} and low loss
} and high loss should have same size.
} I want to use this for comparison, i`m trying to do some
} quantitative EELS
} work but so far with limited success.
} I want to find out wether my program or my data is wrong;)
}
} Thanks,
}
} Jo
}
} *************************************************************
} * Jo Verbeeck *
} * University of Antwerp *
} * Dept. EMAT (Electron Microscopy for Materials Research) *
} * e-mail: joverbee-at-ruca.ua.ac.be *
} * tel: +32(0)3 218 02 49 *
} * fax: +32(0)3 218 02 57 *
} *************************************************************
}
}



From daemon Mon Apr 17 18:35:48 2000



From: Kate Luby-Phelps :      lubyphel-at-SWVX12.SWMED.EDU
Date: Mon, 17 Apr 2000 15:37:28 -0500
Subject: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a JEOL 840A scanning scope with an Oxford Link X-ray analysis
system. We would like to be able to get digital images out of it, for as
few $$ as possible. I would be interested in comments on the following
questions:

1) passive vs. active acquisition (my impression is that the resolution
is better with active, but how much?)

2) relative merits of different commercially available systems (dPict,
4PI, GW Electronics, etc).

3) Are NIH Image and Photoshop sufficient for analysis and processing of
images?

4) relative merits of various X-ray analysis software (such as FLAME,
etc).

Thanks very much.

Kate L-P
--
Kate Luby-Phelps
Molecular & Cellular Imaging Facility
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, TX 75390-9039
e-mail: lubyphel-at-utsw.swmed.edu
Telephone: (214) 648-2190
Fax: (214) 648-6408 or -8885




From daemon Mon Apr 17 19:55:43 2000



From: jeffc07-at-hotmail.com ()
Date: Mon, 17 Apr 2000 19:39:53 -0500
Subject: Ask-A-Microscopist LM of E. coli and P. mirabilis

Contents Retrieved from Microscopy Listserver Archives
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Email: jeffc07-at-hotmail.com
Name: Jeff Courter
School: central michigan universty

Question: my problem is this: I have E. coli and P. mirabilis in 1% low
temperature gelling agarose in spurr's. is there any way to visualize the
specimen to trim it on a microtome other than taking sections individually
and staining them until i find the agar and bacteria.

---------------------------------------------------------------------------




From daemon Mon Apr 17 19:55:43 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 18 Apr 2000 10:50:28 +1000
Subject: RE: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I understand that Shellite is a Shell Petroleum Co trade name, the generic name
in Australia is white gas and this product is very similar to petrol (car fuel)
- except it does not have the certain additives. ( I ran in desperation a
Landcruiser for 50km on that stuff some years ago) It works well enough as a
general solvent for a first degreasing and cleaning of EM parts, especially
pumps. Its quiet cheap to purchase.
Petroleum Ether much more volatile and a more powerful solvents. (more
explosive too)
They may be the same chemically, except that white gas would have a
considerably higher boiling point than Pet. Ether.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Monday, April 17, 2000 8:47 PM, Anaspec [SMTP:anaspec-at-icon.co.za] wrote:
}
}
}
} Hi all
}
} Whilst working in Australia we found a few labs using something called
} Shellite as a de-greaser to clean microscope parts. This works very well and
} seems to be very available. we then looked for the same product here in SA.
} We then found that this is a trade name only in Australia and is actually
} petroleum ether or spirit.
} We called the local Shell distributor to ask what the difference was between
} spirit and ether. Well, they were not sure on that one. Then we were told
} you must specify the temperature range of the petroleum Ether. 40 - 60, 60 -
} 80 or 80 - 100 deg Celsius. Again no explanation as to what the difference
} is an why.
} Can any one give us some more info on Petroleum ether / spirit and the
} advantages disadvantages on the different temperatures.
}
}
} Thanks
}
} Luc Harmsen
} Anaspec, South Africa
} Technical support on microscopy.
} Tel + 27 (0) 11 476 3455
} Fax + 27 (0) 11 476 7290
} anaspec-at-icon.co.za
} www.anaspec.co.za
}
} Remember, ICEM 15 will be in
} 2002, Durban, South Africa.
} www.icem15.com



From daemon Tue Apr 18 07:14:41 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Mon, 17 Apr 2000 22:28:53 -0400 (EDT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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subscribe



Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Apr 18 07:14:45 2000



From: Jill Verlander :      verlaj-at-medicine.ufl.edu
Date: Tue, 18 Apr 2000 09:04:52 -0500
Subject: service on ISI-DS130

Contents Retrieved from Microscopy Listserver Archives
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Kate,
I saw your message on the Listserver. Here is my opinion on Digital
Imaging.

Passive vs. Active - Passive resolution is limited to the maximum
resolution the SEM can produce. If your scan generator has a maximum
resolution of 2000 lines for the photo scan, then your passive resolution
will also be 2000 lines. Passive just simply reads what's there already,
including any character, text and markers. What you see on the Viewing CRT
is "what you get" on the Passive System computer display. One neat thing
that a Passive System can do is grab an image in split screen mode, one side
a SE or BSE Image and the other half an x-ray dot map. An Active System, on
the other hand, takes direct control of the scan coils(replacing the scan
gen. in the sem), and digitizes the resulting video. Systems such as
DIGISEM, can achieve images of 4K x 4K. I believe the 4Pi system goes even
higher. I'm not sure about the rest. Active systems are typically suited
for fast, high quality digital images, that are then viewed and modified
with a program such as PhotoShop. Then, finally you have the standard TV
Rate Frame Grabber. This only works in "TV" mode, and your resolution is
typically limited to the resolution of the SEM TV scan rate(512 x 512?).
Slow scan, either Active or Passive is the way to go. Do it yourself Active
and Passive systems start out around $8,000.00, and of course, go up from
there.

NIH IMAGE was originally written as a MAC application. However, it has
been ported to the PC platform. In the process, the program has lost some
of its functionality. PhotoShop, for both the PC and MAC is a superior
program overall. There are some "shareware" programs out there worth
looking at, such as Paint Shop Pro. It's cheap(less than $100) and can do
just about anything PhotoShop can do(my opinion). Also, be aware that many
imaging systems include image analysis function. This really drives the
price up. If all you are interested in doing is simply grabbing and saving
an image, I would stay away from the "high end" systems.

The 4Pi imaging system is available both in a MAC and PC version. I
think everybody else is PC based.

EDS - What a can of worms. It seems everybody(including myself), offers
a "PC based EDS upgrade". I have personally used the PGT Avalon(formerly
American Nuclear Systems) and 4Pi Flame. The PGT Avalon eXcalibur software
in its latest release is full 32 bit and can run on the Win95/98 and NT
Workstation 4.0. PGT corrected many software problems after purchasing ANS.
Very nice package. The Flame software uses "fuzzy logic" in its approach
to quantitative analysis. The latest rendition of the Flame software seems
pretty good, however, the MAC version is somewhat more stable. Also, an
excellent product, along with 5***** customer support. Keep an eye out on
4Pi though, something new and exciting is in the works. Both are great
systems, inexpensive and easy to operate. Again, the 4Pi system is
available on both the PC and MAC platform. You will find prices on systems
such as these are very close in price to each other. Prices for these
"upgrades" typically start out around $15,000.00.
Good luck in your quest for the right system.

Gary M. Easton
Scanners Corporation
www.scannerscorp.com


Note: I am not employed by any of the companies listed above(except for
Scanners Corporation). However, I do have a commercial interest in these
products, because I sell them and this is how I make a living(along with
servicing SEM's). If I have slighted anybody, or any company, I apologize
in advance. The opinions and facts I have stated here are a result of my
22+ years experience in the scanning electron microscopy field


----- Original Message -----
} From: Kate Luby-Phelps {lubyphel-at-SWVX12.SWMED.EDU}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, April 17, 2000 4:37 PM


Douglas - We have Image Control, Inc., based in Orlando, FL, service our
ISI-DS130C. We don't have a contract, just "as needed" service. I have
been very happy with service and pricing. They do offer service
contracts as well. I don't know what their geographical range is.
Phone number is (407) 234-0676; fax (407) 292-7802.

Jill
Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299



From daemon Tue Apr 18 17:43:33 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Tue, 18 Apr 2000 07:46:58 -0600
Subject: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kate,

let me try to answer some of your questions. I am sending this cc to the
listserver as some of your questions come up on a regular basis.

1) passive vs. active:
Both types of acquisition will give you probably good images. The
difference is, that in a passive system, the computer (or digitizer)
simply digitizes the signals from the microscope. That means, that you
are limited to whatever the microscope can supply in terms of
resolution, dwell time, aspect ratio, etc. Most SEMs have a 1000 line or
2000 line option for taking images. That's what you get. A 1000x1200
image (for 1000 lines), or a 2000x2400 image (for 2000 lines). Plus of
course the other modes of the microscope (slow scan, fast scan, etc). An
active system actually takes control of the scanning coils, usually by
replacing the scan generator during PC controlled acquisition. Thus the
PC can select the resolution, dwell time, aspect ratio, etc. This gives
you much more freedom in selecting an image format, plus it is much
easier to acquire X-ray dot maps with an existing X-ray system. Typical
max. resolutions are 4000x4000. These big images take time to acquire,
though (seconds). Installation of a passive system may be easier in some
circumstances, but on your 840 the installation of an active system is
"plug and play".

2) different commercial systems.
As we sell our own system (ADDA II), and since this goes to the
listserver, I don't want to comment on this question. Call me if you
need information about our system.

3) Software:
Of the two programs you mention, I would prefer NIH. Photoshop is mainly
for making photos look nicer and may not have all the tools you require
(unless you buy other add-ons). Buy the software, however, with an eye
on expansion and support. Our experience with other users is, that once
they have added a digital acquisition system, they quickly find other
things they can do with the system and sometimes need more software or
hardware. For example, they realize that the old light microscope used
for specimen preparation can be digitized too and be used much better
than before. That, for example, would require a camera. So, if that is
the case, you want to look for a system that also supports cameras.
Another issue is the distribution of images. Many of our customers have
predefined "reports" for their customers. In that case you want to look
for software that supports the creation of custom templates, not just
printing images. An embedded, network capable database with search
capabilities is a big plus if you have several users.

4) X-ray software:
This is outside my area of expertise, so I will refrain from making any
comments.

I hope, this helps you decide about your SEM. If you have more detailed
questions, please send me an email or call me.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

*******************************************************
Disclaimer:
Soft Imaging System produces and sells image acquisition
and processing systems. We therefore have a vested
interest in some of the items mentioned above.
*******************************************************


-----Original Message-----
} From: Kate Luby-Phelps [mailto:lubyphel-at-SWVX12.SWMED.EDU]
Sent: Monday, April 17, 2000 2:37 PM
To: Microscopy-at-sparc5.microscopy.com


We have a JEOL 840A scanning scope with an Oxford Link X-ray analysis
system. We would like to be able to get digital images out of it, for as
few $$ as possible. I would be interested in comments on the following
questions:

1) passive vs. active acquisition (my impression is that the resolution
is better with active, but how much?)

2) relative merits of different commercially available systems (dPict,
4PI, GW Electronics, etc).

3) Are NIH Image and Photoshop sufficient for analysis and processing of
images?

4) relative merits of various X-ray analysis software (such as FLAME,
etc).

Thanks very much.

Kate L-P
--
Kate Luby-Phelps
Molecular & Cellular Imaging Facility
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, TX 75390-9039
e-mail: lubyphel-at-utsw.swmed.edu
Telephone: (214) 648-2190
Fax: (214) 648-6408 or -8885




From daemon Tue Apr 18 17:43:34 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 18 Apr 2000 10:30:09 -0400
Subject: Re: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


jim wrote:

Dear Jim,

} the generic name
} in Australia is white gas and this product is very similar to petrol (car fuel)
} - except it does not have the certain additives.

White gas is the petroleum distillate fraction used to make petrol.


} They may be the same chemically, except that white gas would have a
} considerably higher boiling point than Pet. Ether.

White gas contains hydrocarbons that have a boiling point roughly
the same as that of octane. There are some chemical differences, since the
higher-boiling-point fractions will contain more complex mixtures than
the lower-boiling-point fractions. In particular, the lowest such fraction,
which contains mostly pentanes, has no aromatics.
Yours,
Bill Tivol



From daemon Tue Apr 18 17:43:35 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 18 Apr 2000 10:44:33 -0400 (EDT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe Microscopy dmrelion-at-world.std.com




Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Apr 18 17:43:36 2000



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Tue, 18 Apr 2000 07:53:22 -0700 (PDT)
Subject: For C Singla

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following the thread on processing tissue-culture
cells for TEM, would C Singla reply back to me if they
would like a protocol for embedding cell monolayers
for TEM in the petri-dish.

Jeremy Sanderson

jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Send online invitations with Yahoo! Invites.
http://invites.yahoo.com



From daemon Tue Apr 18 17:43:38 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Tue, 18 Apr 2000 09:28:54 -0600
Subject: LM - tissue processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.

Peace be with you,
Phil Rutledge (410)778-4136, 2120
prutledge-at-ars.usda.gov







From daemon Tue Apr 18 17:43:39 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 18 Apr 2000 11:43:28 -0400 (EDT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe Microscopy dmrelion-at-world.std.com



From daemon Tue Apr 18 17:43:48 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Tue, 18 Apr 2000 11:44:30 -0700
Subject: Folding Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been using folding grids for my sections for a long time (200 mesh,
Cu). Lately however I have found it very difficult to fold the grids so
that the two sides overlap properly and only a few of the holes are in good
registry, the rest are partially blocked by the overlap. I am wondering if
this is a manufacturing problem ( i.e. a bad batch ?) . Has anyone else
experienced this problem with a batch of grids ? Suggestions are welcome.

Thanks,

Jordi Marti



From daemon Tue Apr 18 17:43:49 2000



From: Jeff Doran :      jeffd-at-di.com
Date: Tue, 18 Apr 2000 13:36:39 -0700
Subject: AFM seminar/workshop at Princeton University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There will be an electrical characterization by AFM seminar/workshop sponsored
by Digital Instruments and Princeton University on April 25, 2000. For
additional information and registration, please visit the DI web site at
www.di.com and click on "workshops and seminars".

Regards,
Jeff Doran
Digital Instruments
Veeco Metrology Group





From daemon Tue Apr 18 17:43:50 2000



From: Jeff Doran :      jeffd-at-di.com
Date: Tue, 18 Apr 2000 13:35:08 -0700
Subject: AFM Seminar/Workshop at Princeton University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There will be an electrical characterization by AFM seminar/workshop sponsored
by Digital Instruments and Princeton University on April 25, 2000. For
additional information and registration, please visit the DI web site at
www.di.com and click on "workshops and seminars".

Regards,
Jeff Doran
Digital Instruments
Veeco Metrology Group





From daemon Tue Apr 18 17:43:50 2000



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Tue, 18 Apr 2000 16:44:35 -0500
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} though (seconds). Installation of a passive system may be easier in some
} circumstances, but on your 840 the installation of an active system is
} "plug and play".

Actually, about 90% of the 840s have the internal scan relays
soldered in place on the scan gen board. The other 10% will need the relays
added. JEOL service is best for this as they have the relays in stock. To
check, one must physically examine the scan gen board.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Tue Apr 18 17:43:51 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 18 Apr 2000 16:06:51 -0500
Subject: Value of a used Hitachi S-570

Contents Retrieved from Microscopy Listserver Archives
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Anyone have an idea what the sale value might be for a Hitachi S-570
with a LaB6 gun & solid-state backscatter detector? No x-ray. Comes
with a Gatan digital imaging system. Sorry, but I don't know the
model of this system, but it uses a PowerMac with a NuBus card, so
it's a bit hoary.

In good condition, recently PMed to give "to spec." performance in
the upper stage at 200,000X.

Thanks.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue Apr 18 17:43:52 2000



From: Lewis McCrigler :      lmm7001-at-Humboldt.edu
Date: Tue, 18 Apr 2000 14:17:00 -0700
Subject: Help on an Orthomat E Photo System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I'm looking for technical assistance or a service manual for a Leitz
Orthomat E (7916) Photo System.The problem we are having is the unit will
not allow a picture to be taken unless there is very little light. Once the
system thinks it is in range, there is not enough light present to get the
photograph. An adjustment of the light detector? Any assistance is
appreciated.
Lewis McCrigler
Humboldt State University



From daemon Wed Apr 19 08:05:27 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Mon, 17 Apr 2000 09:21:05 +1000
Subject: Re: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day Luc,
Shellite is a solvent manufactured by Shell, its used as stove fuel,
lighter fuel, drycleaning solvent and as a rubber/adhesive solvent
(also, as you have found, a great EM parts cleaner). Another name you
may find Shellite listed under is Shell X55 solvent.
Shellite is basically a highly volitile, low octane, unleaded petrol.
According to Shell Australia it is not the same as white spirit, this is
less volitile than shellite, therefore harder to evaporate from the
metal surface.
Below is part of the safety data sheet for Shellite. If anyone requires
the complete 10 page safty data sheet please email me.
Luc, see you next time you are in OZ.
Regards
JVN
SHELL SHELlLITE

STATEMENT OF HAZARDOUS NATURE

HAZARDOUS ACCORDING TO WORKSAFE AUSTRALIA CRITERIA

SUPPLIER

Company: The Shell Company of Australia Limited
Address: Shell House, 1 Spring Street (PO Box 872K) PO Box 2091
Melbourne Wellington
VIC 3001 New Zealand
Australia
Telephone: (03)9666 5444
Telephone: 64 4 4720080
Fax: (03)96665008/64 44980100


HAZARD RATINGS

Flammability: 4

Toxicity: 2

Body Contact: 2

Reactivity: 0

Chronic effect: 2

Scale: Min / Nil = 0, Low = 1, Moderate = 2, High = 3 and Extreme = 4.

PERSONAL PROTECTIVE EQUIPEMENT FOR
INDUSTRIAL/COMMERCIAL ENVIRONMENTS

Product Name:
Shell Shellite
Other Names:
Product Code 00720


CAS RN No(s):
None
U.N. Number:
1268
Packaging Group:
II
Dangerous Goods Class:
3.1
Subsidiary Risk:
None
Hazchem Code:
3[Y]E
Poisons Schedule Number:
S5


USE

Used as rubber solvent, cleaning solvent, lighter fluid and as fast
evaporating, highly volatile solvent in
enamels, adhesives and lacquers. The use of a quantity of material in an
unventilated or confined
space may result in increased exposure and an irritating atmosphere
developing Before starting
consider control of exposure by mechanical ventilation.

PHYSICAL DESCRIPTION/PROPERTIES

APPEARANCE

Clear highly flammable liquid with a typical hydrocarbon liquid odour;
floats on water. Classed as an
aliphatic solvent; i.e has low aromatic content.

Molecular Weight:
Not applicable.
Boiling Point (deg C):
47-128
Melting Point (deg C):
Not available.
Vapour Pressure (kPa):
34.5 -at- 15 deg C
Specific Gravity:
0.71 -at- 15 deg C
Flash Point (deg C):
{-30
Lower Explosive Limit (%):
1.0
Upper Explosive Limit (%):
7.5
Solubility in Water (g/L):
Immiscible


INGREDIENTS

NAME
CAS RN
%
paraffins, as
liquid hydrocarbons
Various
} 60
naphthenes
Notspec
n-hexane
110-54-3
13
aromatic hydrocarbons total, including
{5.0
toluene
108-88-3
3.5app
ethylbenzene
100-41-4
benzene
71-43-2
{0.5
C8 and higher aromatics
approx1

Anaspec wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all
}
} Whilst working in Australia we found a few labs using something called
} Shellite as a de-greaser to clean microscope parts. This works very well and
} seems to be very available. we then looked for the same product here in SA.
} We then found that this is a trade name only in Australia and is actually
} petroleum ether or spirit.
} We called the local Shell distributor to ask what the difference was between
} spirit and ether. Well, they were not sure on that one. Then we were told
} you must specify the temperature range of the petroleum Ether. 40 - 60, 60 -
} 80 or 80 - 100 deg Celsius. Again no explanation as to what the difference
} is an why.
} Can any one give us some more info on Petroleum ether / spirit and the
} advantages disadvantages on the different temperatures.
}
} Thanks
}
} Luc Harmsen
} Anaspec, South Africa
} Technical support on microscopy.
} Tel + 27 (0) 11 476 3455
} Fax + 27 (0) 11 476 7290
} anaspec-at-icon.co.za
} www.anaspec.co.za
}
} Remember, ICEM 15 will be in
} 2002, Durban, South Africa.
} www.icem15.com

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Wed Apr 19 08:05:36 2000



From: Ford Royer :      froyer-at-bitstream.net
Date: Tue, 18 Apr 2000 21:13:05 -0500
Subject: Re: LM - tissue processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I carry a full line of refurbished histology equipment. Please contact me for price quotes and recommendations.

Ford Royer
Analytical Instruments
9921 13th Ave. N.
Minneapolis, MN 55441
(800) 565-1895, extension 17
fax: (612) 929-1895
email: froyer-at-bitstream.net

Phillip Rutledge wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.
}
} Peace be with you,
} Phil Rutledge (410)778-4136, 2120
} prutledge-at-ars.usda.gov



From daemon Wed Apr 19 08:05:38 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 19 Apr 2000 16:10:51 GMT+1200
Subject: White Spirit

Contents Retrieved from Microscopy Listserver Archives
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Just to throw in a further little complication of nomenclature:

In New Zealand, (and maybe in Australia, too), the general name we
use for stuff like Shellite, is "white spirit".

In the US it's often called "unleaded gas(oline)", or "white
gas(oline)", I think.

It's the stuff that Coleman camping stoves run on.

However, in the UK, "white spirits" is what I would call "mineral
turpentine" ie the comparitively non-volatile solvent often used for
thinning oil-based paints.

As I found a few years ago, shortly after my arrival in the UK to
continue the camping holiday that I'd started in the US, it doesn't
work in Coleman stoves.

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Apr 19 08:06:08 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Wed, 19 Apr 2000 09:16:35 +0200
Subject: TEM of clusters and image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

we have recently started working on Transmission Electron Microscopy of
several kinds of clusters in various martices.
We frequently face the problem of analyzing particle size distribution and
other features of the images.

We use several software programs for our particle analysis, but we always
conclude that measuring the particle size by eye is the most reliable way
to get accurate data.

We have both Digital Micrograph software and other programs (Scion image
etc...), but we have never used them for this kind of application.

As we look both at bulk materials, and at thin films or powders, frequently
our images show an uneven contrast, and defining a threshold is difficult.

Can anyone suggest (and eventually share) a script, a program or a set of
user functions that allows to study particle size distributions?

Thank very much in advance for your help in this matter.

Max

Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm






From daemon Wed Apr 19 08:06:11 2000



From: Sidorov, Max :      msv-at-nl.feico.com
Date: Wed, 19 Apr 2000 13:10:05 +0200
Subject: RE: TEM of clusters and image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Massimo:
Try to Fourier-filter (high-pass) the images before doing the particle
analysis. In this way you might be able remove the influence of the uneven
background. Sometimes this works nicely. You can easily do it in Digital
Micrograph.
Hope this helps,

Max Sidorov
----------------------------
Dr. Maxim V. Sidorov
TEM Applications Specialist
Philips Electron Optics, Applications Laboratory
Building AAE, Achtseweg Noord 5
5600 MD Eindhoven, the Netherlands

e-mail: msv-at-nl.feico.com
Phone: +31-40/2766101
Fax: +31-40/2766102



} -----Original Message-----
} From: Massimo Catalano [SMTP:massimo.catalano-at-ime.le.cnr.it]
} Sent: Wednesday, April 19, 2000 09:17
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM of clusters and image analysis
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listservers,
}
} we have recently started working on Transmission Electron Microscopy of
} several kinds of clusters in various martices.
} We frequently face the problem of analyzing particle size distribution and
}
} other features of the images.
}
} We use several software programs for our particle analysis, but we always
} conclude that measuring the particle size by eye is the most reliable way
} to get accurate data.
}
} We have both Digital Micrograph software and other programs (Scion image
} etc...), but we have never used them for this kind of application.
}
} As we look both at bulk materials, and at thin films or powders,
} frequently
} our images show an uneven contrast, and defining a threshold is difficult.
}
} Can anyone suggest (and eventually share) a script, a program or a set of
}
} user functions that allows to study particle size distributions?
}
} Thank very much in advance for your help in this matter.
}
} Max
}
} Dr. Massimo Catalano
} CNR-IME
} Campus Universitario
} Via Arnesano
} 73100 Lecce - ITALY
} tel: + 39 0832 322362
} fax: + 39 0832 325299
} email: massimo.catalano-at-ime.le.cnr.it
} http://www.ime.le.cnr.it
} http://www.ime.le.cnr.it/sime/sime.htm
}
}
}
}



From daemon Wed Apr 19 08:44:13 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Wed, 19 Apr 2000 08:28:05 -0500
Subject: LEO 440i SEM and cathodoluminescence (CL)

Contents Retrieved from Microscopy Listserver Archives
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To SEM users and vendors

On a recent business trip outside the US, I met some people who are using a
LEO 440i SEM. They wanted to do cathodoluminescence (CL) with it and were
not having too much success so I volunteered to post this message to the
list to seek advice. The PMT is mounted in a housing/port that is located
at
about 2 O'clock (if you consider the door to be at 6 o'clock). The mounting
of the PMT is horizontal and it looks like the distance to the PMT input is
about 20 cm from the center of the chamber. There is a glass lens on a
slider arrangement in front of the PMT input but they were unclear as to
how
to adjust this.

They do not know the type of PMT that was supplied and haven't opened up
the system to determine this. It looks like it must be an end-on type.

One of their questions has to do with the adjustment, if any, for the PMT
voltage and how they can do this and how they can monitor the value of the
PMT voltage.

They believe that the PMT should have been mounted at an angle to better
see
the sample but the only other port where this could be done is not usable
for the PMT when the EDS detector is in place.

The other work that they are doing with the instrument is completely
satisfactory, so far as I could tell, and I suspect that someone on the
list
can provide the answers to these questions and help to get them in business
doing monochromatic CL as well.

All suggestions from ohter users of this type of instrument, obviously,
will
be much appreciated.

Any vendors who provide CL accessories for this instrument should contact
me
directly.

Thank you.

Donald J. Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead,
send it to donbarlen-at-aol.com. Thank you.)




From daemon Wed Apr 19 18:24:03 2000



From: Jeff Doran :      jeffd-at-di.com
Date: Wed, 19 Apr 2000 07:23:57 -0700
Subject: AFM Seminar/Workshop at PMI

Contents Retrieved from Microscopy Listserver Archives
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Please note that the Seminar/Workshop at Princeton University on April 25, 2000
will be at PMI. For additional information and registration please see the DI
web site at www.di.com and click on "Workshops and Seminars".

Regards,
Jeff Doran
Digital Instruments
Veeco Metrology Group



From daemon Wed Apr 19 18:24:04 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Wed, 19 Apr 2000 09:15:13 -0500
Subject: Re: White Spirit

Contents Retrieved from Microscopy Listserver Archives
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Hi Richie & readers
As I recall from the oil field days of the 80s, the term "white
gasoline" referred to a condensate from natural gas production. People were
known to collect (swipe) this free fuel at the well site & use it in their
autos. I do not know what it's composition is but this was not really
considered a good substitute for gasoline. Something about destroyed pistons
& the such.

Bruce Brinson
Rice U.

Ritchie Sims wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Just to throw in a further little complication of nomenclature:
}
} In New Zealand, (and maybe in Australia, too), the general name we
} use for stuff like Shellite, is "white spirit".
}
} In the US it's often called "unleaded gas(oline)", or "white
} gas(oline)", I think.
}
} It's the stuff that Coleman camping stoves run on.
}
} However, in the UK, "white spirits" is what I would call "mineral
} turpentine" ie the comparitively non-volatile solvent often used for
} thinning oil-based paints.
}
} As I found a few years ago, shortly after my arrival in the UK to
} continue the camping holiday that I'd started in the US, it doesn't
} work in Coleman stoves.
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Wed Apr 19 18:24:05 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Wed, 19 Apr 2000 08:39:20 -0600
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, but Kate said, that they already have an EDX system attached.
Normally these systems need access to the scan coils as well, that's why
I mentioned "plug and play". I bet, that the EDX system is connected to
connector JA2 on the back of the microscope, which is where we would
also connect the digital acquisition system (of course with an
additional connector for the existing X-ray system).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Scott D. Davilla [mailto:davilla-at-4pi.com]
Sent: Tuesday, April 18, 2000 3:45 PM
To: Microscopy-at-sparc5.microscopy.com


} though (seconds). Installation of a passive system may be easier in
some
} circumstances, but on your 840 the installation of an active system is
} "plug and play".

Actually, about 90% of the 840s have the internal scan relays
soldered in place on the scan gen board. The other 10% will need the
relays
added. JEOL service is best for this as they have the relays in stock.
To
check, one must physically examine the scan gen board.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Apr 19 18:24:07 2000



From: Chris Edwards :      fishon-at-umich.edu
Date: Wed, 19 Apr 2000 11:42:18 -0400
Subject: Zeiss Axiocam

Contents Retrieved from Microscopy Listserver Archives
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Has anyone had a demo for the new Zeiss CCD known as the "Axiocam"? Or
better, has anyone ordered it and had it installed in their lab. I'd like
to know the usual things regarding performance and overall satisfaction.
Thanks.




From daemon Wed Apr 19 18:24:08 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 19 Apr 2000 17:06:23 +0000
Subject: Cause of turbo pump failure

Contents Retrieved from Microscopy Listserver Archives
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Some of you asked what caused the turbo pump failure I related
last week. I frankly don't know. There is some scope in the S250
for objects (samples, for example) to fall into the pump. Cambridge
fitted a mesh screen to trap these, but it is possible that some
small hard fragment got between the fan and stator blades
somehow. In early Cambridge 250s there was no rough pumping
sequence - the baffle valve was simply opened, dumping the
specimen chamber air at 1 bar into the turbo pump while it was
running at its top speed. The dramatic shreik of protest that
results is a great party trick when demonstrating the machine to
visitors, but slows the pump dramatically and undoubtedly causes
great mechanical stress on the turbines and stator blades.
Strangely, we never suffered pump failures during this stressful
event, but only when the pumps were in apparently smooth running
at top speed. I think we are now on about our fourth pump in
twenty years, and this last one has survived unscathed for about 10
years with nothing more than an occasional oil-change, during
which time it has mostly been run continuously, surviving endless
air-dump cycles. Now that I have told you that I can probably
expect our next failure almost immediately! Perhaps I'll order the
skip now, just in case.
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Wed Apr 19 18:24:09 2000



From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Wed, 19 Apr 2000 11:22:23 -0500 (CDT)
Subject: Re: Zeiss Axiocam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

I saw the scope several weeks back but never saw through it, Zeiss could
not get it to work. I know one lab on campus has one and they say it
images beautifully, when it works...... they have been using our
Optronics lately.

Thomas Moninger moninger-at-emiris.iaf.uiowa.edu
University of Iowa Central Microscopy Research Facility
http://www.uiowa.edu/~cemrf
Views expressed are mine.
On Wed, 19 Apr 2000, Chris Edwards wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Has anyone had a demo for the new Zeiss CCD known as the "Axiocam"? Or
} better, has anyone ordered it and had it installed in their lab. I'd like
} to know the usual things regarding performance and overall satisfaction.
} Thanks.
}
}
}



From daemon Wed Apr 19 18:24:09 2000



From: Don Grimes :      microtoday-at-mindspring.com
Date: Wed, 19 Apr 2000 12:21:46 -0500
Subject: Just For Fun Image Contest

Contents Retrieved from Microscopy Listserver Archives
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Readers,
At the upcoming Microscopy & Microanalysis Conference (13/17 August in
Philadelphia) we will again hold a Just For Fun Image Contest. Concept is an
image composed of one or more other images, one of which must be
microscopical in nature.
Prizes will be $300, $200 and $100 for first, second and third prizes
respectively.
One does not have to be present to win.
Should you might be interested, kindly contact me direct and I will forward
detail.
Last year we had over 30 entries.
Regards,
Don Grimes, Microscopy Today



From daemon Wed Apr 19 18:24:13 2000



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Wed, 19 Apr 2000 13:30:55 -0500
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Yes, but Kate said, that they already have an EDX system attached.
} Normally these systems need access to the scan coils as well, that's why
} I mentioned "plug and play". I bet, that the EDX system is connected to
} connector JA2 on the back of the microscope, which is where we would
} also connect the digital acquisition system (of course with an
} additional connector for the existing X-ray system).

Maybe you misunderstand my intent. All I'm saying is don't assume a
JEOL 840 has everything required for active scan control. Some don't, but
it's not a show stopper provided the information is known and planned for
in advance.
Just because there is an EDX system attached does not mean that it
is attached to the microscope beam control. There are more EDX systems sold
without X-ray mapping than sold with X-ray mapping. In the same note, just
because the JEOL 840 has a "plug and play" interface does not mean that the
external scan relays are present. Both issues raise flags here and can mean
the difference in a painless or painfull installation if the details are
not addressed.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Apr 19 18:24:17 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 19 Apr 2000 15:28:58 -0500
Subject: Value of the S-570

Contents Retrieved from Microscopy Listserver Archives
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Many thanks for the various valuations sent me. We have enough now
for our needs.
For those interested, the valuations ranged from 15k$ to 45k$, with
the average about 25 - 30 k$.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Wed Apr 19 18:24:17 2000



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Wed, 19 Apr 2000 14:57:38 -0700
Subject: Digital printer needs repair (Kodak XLS8300)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Kodak XLS8300 dye sublimation printer that needs repair. Kodak
no longer supports this instrument, has no replacement parts we need and
doesn't know where we can get service.

When we shipped it for repair, we got a report that said it needed a hard
drive and possibly a saturn board (motherboard, I presume) and that they
could not provide service.

I would appreciate getting suggestions of where to get this printer serviced.

Please reply directly to me, and not to the listserv.

Thanks,

John
chandler-at-colostate.edu




From daemon Wed Apr 19 18:24:18 2000



From: Steven Ridge :      s.ridge-at-fryerco.com
Date: Wed, 19 Apr 2000 16:10:41 -0500
Subject: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can someone help me with a US source to purchase, low fluorescence
quartz coverlips for conventional microscope slides. Please respond
directly at the following email address.

Steven Ridge
Fryer Company Inc.
847-669-2000 Phone
s.ridge-at-fryerco.com

--
BMv



From daemon Wed Apr 19 18:55:07 2000



From: Joe :      jmetzger-at-suscom.net
Date: Wed, 19 Apr 2000 19:51:19 -0400
Subject: Xray

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently completed a research paper to complete my EE
Degree which included a section on vacuum principles. I
incorporated part of the in formation I got from an article
by XEI scientific on contamination control. It spoke of oil
deposits on cool windows or a loss of low energy xray. I
thought I also read (on the web site) of how x-rays will
turn ceramics yellow. I can not find the article again.
Can you help me with this? What I am trying to decipher is,
will concentrated xray emission through a ceramic cause it
to yellow and could contamination deposits be the cause of
this? Would appreciate any answers or leads as to where I
might find the answer.

Sincerely
Joe Metzger




From daemon Wed Apr 19 19:04:30 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 19 Apr 2000 19:58:17 -0400
Subject: Extra rooms for MRS in San Francisco

Contents Retrieved from Microscopy Listserver Archives
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Once again there is a conference coming up and I have found myself with a
few extra rooms. If anyone is need of hotel rooms at the San Francisco
Marriott for the Materials Research Society meeting next week, please let
me know. If nobody needs them, I will be cancelling them tomorrow night.

I have rooms for arrival on Saturday April 22 and departure on Friday April
28. Of course the dates could be changed if needed. They are rooms with 2
double beds and the confrence rate is $138 I think.

Let me know ASAP.

Best regards-

David
Writing at 4:52:02 PM on 04/19/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.



From daemon Thu Apr 20 07:37:23 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, April 19, 2000 5:59 PM
Subject: Xray

Contents Retrieved from Microscopy Listserver Archives
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Dear Joe and list members:

I did not say anything about yellowing of ceramics at the XEI Scientific web
site. It is generally not a "contamination" problem. X-rays being an
ionizing radiation are able to break bond and modify the crystalline
structure of ceramics. When I did X-ray spectrometry I was very aware of the
ability of high intensity, high energy x-rays to cause radiation damage and
discoloration. However scanning electron microscope are generally operated
at low energies ( {30KeV) and low beam currents. Xray damage is not a problem
except with most sensitive materials.

Xray damage to oils could cause them to crosslink and yellow if oils are
deposited on the surface of ceramic but I have not seen any complaints about
this. Maybe a more specific description of your problem would help. Reply
off list.

Notice: XEI Scientific sells anti-contamination systems.

Ronald Vane
XEI Scientific

-----Original Message-----
} From: Joe {jmetzger-at-suscom.net}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Thu Apr 20 07:37:24 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 19 Apr 2000 21:33:07 -0700
Subject: Re: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
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At 02:10 PM 4/19/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Rreally....can anyone identify a source of high quality
#1 cover slips that are not made in China and don't have
micro fractures in them?

gg



From daemon Thu Apr 20 17:36:19 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 20 Apr 2000 22:32:57 +1000
Subject: RE: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Coverslip number 1 thickness is 0.13 to 0.17mm. Ours are 0.25 thick (and 25.4mm
dia)
I didn't know #1 quartz coverslips existed, made in China or outer space.
Perhaps GG should not blame the Chinese manufacturer for flaws in #1 slips;
maybe perfect #1 in quartz are impossible.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, April 20, 2000 2:33 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
}
}
} At 02:10 PM 4/19/00 , you wrote:
}
} } Can someone help me with a US source to purchase, low fluorescence
} } quartz coverlips for conventional microscope slides. Please respond
} } directly at the following email address.
} }
} } Steven Ridge
} } Fryer Company Inc.
} } 847-669-2000 Phone
} } s.ridge-at-fryerco.com
} }
} } --
} } BMv
}
}
} Rreally....can anyone identify a source of high quality
} #1 cover slips that are not made in China and don't have
} micro fractures in them?
}
} gg
}



From daemon Thu Apr 20 17:36:19 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 20 Apr 2000 07:40:33 -0500
Subject: RE: White Spirit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Friends:
I have always wondered about these solvents. One way to sort out
intercontinental differences is to ask - what do they smell like? Gasoline
has a distinctive odor, whether leaded or not. The Pet Ether I use in my lab
is nearly odorless. The label describes it as having a "boiling range of
37.7 to 55.8 C/ 1 drop to dryness." Turpentine, or more correctly, oil of
turpentine, has another distinctive odor. It is used to thin paints.
Petroleum paint thinner, a good solvent, is much like pet ether, nearly
odorless. All of the above are colorless, Kerosene, used in camp stoves and
cabin heaters, another petroleum derivative, is yellow and possesses its own
characteristic odor. All of these observations are from a US view point.
How do these other solvents smell?

Sam Purdy
National Steel Corp Tech Center
Trenton, MI, USA
spurdy-at-nationalsteel.com

} ----------
} From: Ritchie Sims
} Sent: 19, April 2000, 12:10 PM
} To: 'MSA listserver'
} Subject: White Spirit
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Just to throw in a further little complication of nomenclature:
}
} In New Zealand, (and maybe in Australia, too), the general name we
} use for stuff like Shellite, is "white spirit".
}
} In the US it's often called "unleaded gas(oline)", or "white
} gas(oline)", I think.
}
} It's the stuff that Coleman camping stoves run on.
}
} However, in the UK, "white spirits" is what I would call "mineral
} turpentine" ie the comparitively non-volatile solvent often used for
} thinning oil-based paints.
}
} As I found a few years ago, shortly after my arrival in the UK to
} continue the camping holiday that I'd started in the US, it doesn't
} work in Coleman stoves.
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}




From daemon Thu Apr 20 17:36:20 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 20 Apr 2000 06:17:41 -0700
Subject: RE: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK, if not quartz, how about glass? The Chinese products
come pre-made with micro cracks. Erie used to make slips
but stopped. I can find round slips from Swiss glass but not
square ones for 1" x 3" slides.

I think that 4 attempts with Chinese covers which all have the
same problem is not a coincidence. These were bought from
Ward's Scientific. They exchanged them without any hassle.
The problem is that the slips are un useable. I'd be glad to
send a few new ones to you so you can see the cracks for
yourself.

gary g.


At 05:32 AM 4/20/00 , you wrote:
} Coverslip number 1 thickness is 0.13 to 0.17mm. Ours are 0.25 thick (and
} 25.4mm
} dia)
} I didn't know #1 quartz coverslips existed, made in China or outer space.
} Perhaps GG should not blame the Chinese manufacturer for flaws in #1 slips;
} maybe perfect #1 in quartz are impossible.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Thursday, April 20, 2000 2:33 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
} wrote:
} }
} }
} } At 02:10 PM 4/19/00 , you wrote:
} }
} } } Can someone help me with a US source to purchase, low fluorescence
} } } quartz coverlips for conventional microscope slides. Please respond
} } } directly at the following email address.
} } }
} } } Steven Ridge
} } } Fryer Company Inc.
} } } 847-669-2000 Phone
} } } s.ridge-at-fryerco.com
} } }
} } } --
} } } BMv
} }
} }
} } Rreally....can anyone identify a source of high quality
} } #1 cover slips that are not made in China and don't have
} } micro fractures in them?
} }
} } gg
} }



From daemon Thu Apr 20 17:36:21 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 20 Apr 2000 23:50:47 +1000
Subject: RE: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I cannot believe that only Chinese coverslips are sold in the USA. We carry a
comprehensive range of good German-made coverslips . Yes, and they don't have
common flaws.
If that sounds too much like an advertisement, I'll add that several suppliers
in Australia offer a similar range. Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, April 20, 2000 11:18 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} OK, if not quartz, how about glass? The Chinese products
} come pre-made with micro cracks. Erie used to make slips
} but stopped. I can find round slips from Swiss glass but not
} square ones for 1" x 3" slides.
}
} I think that 4 attempts with Chinese covers which all have the
} same problem is not a coincidence. These were bought from
} Ward's Scientific. They exchanged them without any hassle.
} The problem is that the slips are un useable. I'd be glad to
} send a few new ones to you so you can see the cracks for
} yourself.
}
} gary g.
}
}
} At 05:32 AM 4/20/00 , you wrote:
} } Coverslip number 1 thickness is 0.13 to 0.17mm. Ours are 0.25 thick (and
} } 25.4mm
} } dia)
} } I didn't know #1 quartz coverslips existed, made in China or outer space.
} } Perhaps GG should not blame the Chinese manufacturer for flaws in #1 slips;
} } maybe perfect #1 in quartz are impossible.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Thursday, April 20, 2000 2:33 PM, Dr. Gary Gaugler
[SMTP:gary-at-gaugler.com]
} }
} } wrote:
} } }
} } }
} } } At 02:10 PM 4/19/00 , you wrote:
} } }
} } } } Can someone help me with a US source to purchase, low fluorescence
} } } } quartz coverlips for conventional microscope slides. Please respond
} } } } directly at the following email address.
} } } }
} } } } Steven Ridge
} } } } Fryer Company Inc.
} } } } 847-669-2000 Phone
} } } } s.ridge-at-fryerco.com
} } } }
} } } } --
} } } } BMv
} } }
} } }
} } } Rreally....can anyone identify a source of high quality
} } } #1 cover slips that are not made in China and don't have
} } } micro fractures in them?
} } }
} } } gg
} } }



From daemon Thu Apr 20 17:36:22 2000



From: ComCryLab1-at-aol.com
Date: Thu, 20 Apr 2000 10:18:03 EDT
Subject: Re: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Can someone help me with a US source to purchase, low fluorescence
} quartz coverlips for conventional microscope slides. Please respond
} directly at the following email address.
}
} Steven Ridge
} Fryer Company Inc.
} 847-669-2000 Phone
} s.ridge-at-fryerco.com
}
Steven,
We can make anything you need. Email, fax or call with specifications.
Mike Urbanik
guru-at-crystalguru.com
www.crystalguru.com
Ph 941-645-5959
Fax 941-643-6058



From daemon Thu Apr 20 17:36:22 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Thu, 20 Apr 2000 08:12:36 -0600
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

I don't think we're in disagreement here. We've had our share of
surprises (SEMs with external connectors for beam control, then nothing
attached to the connectors, strange boards in the microscopes that
should not have been there, etc.) We have had very few problems with the
840 (and 820, 6300, 6400 for that matter). But of course there is always
a possibility that something is missing or not working, which must be
ascertained in each case individually. As you said, it is usually not a
show stopper, but requires some modifications of the microscope.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Scott D. Davilla [mailto:davilla-at-4pi.com]
Sent: Wednesday, April 19, 2000 12:31 PM
To: Microscopy-at-sparc5.microscopy.com


} Yes, but Kate said, that they already have an EDX system attached.
} Normally these systems need access to the scan coils as well, that's
why
} I mentioned "plug and play". I bet, that the EDX system is connected to
} connector JA2 on the back of the microscope, which is where we would
} also connect the digital acquisition system (of course with an
} additional connector for the existing X-ray system).

Maybe you misunderstand my intent. All I'm saying is don't
assume a
JEOL 840 has everything required for active scan control. Some don't,
but
it's not a show stopper provided the information is known and planned
for
in advance.
Just because there is an EDX system attached does not mean that
it
is attached to the microscope beam control. There are more EDX systems
sold
without X-ray mapping than sold with X-ray mapping. In the same note,
just
because the JEOL 840 has a "plug and play" interface does not mean that
the
external scan relays are present. Both issues raise flags here and can
mean
the difference in a painless or painfull installation if the details are
not addressed.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Thu Apr 20 17:36:23 2000



From: Filion, Christian :      christian.filion-at-atlasstainless.com
Date: Thu, 20 Apr 2000 10:21:13 -0400
Subject: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,

I have both a Spectra file in Link format and txt format saved as a MSA
type file. Is there a software or an excel macro that could read these
spectra ?

Thank you

Christian Filion
Superviseur Laboratoires
Aciers Inoxydables Atlas
1640 Marie-Victorin
Tracy, QuŽbec
J3R 5R5
TelÊ: 450-746-5243
faxÊ: 450-746-5241



From daemon Thu Apr 20 17:36:23 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 20 Apr 2000 10:51:25 -0500
Subject: Quartz coverslips availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Steven Ridge wrote:
==========================================================
Can someone help me with a US source to purchase, low fluorescence quartz
coverlips for conventional microscope slides. Please respond directly at
the following email address.
===========================================================
SPI Supplies has offered quartz coverslips 0.2 mm thick for some time and
the details can be found on URL
http://www.2spi.com/catalog/ltmic/quartz.html

The optical characteristics of (fused) quartz are very very sensitive to the
impurity levels present. Therefore it is important that one use coverslip
that are of reproducible quality and of known impurity levels (also linked
from above webpage). The particular quartz used for the manufacture of the
SPI quartz coverslips is the same "electronic grade" used in many
applications in the electronics industry. The coverslips and quartz are of
USA manufacture (just in case one might wonder about origin).

Disclaimer: SPI Supplies has supplied quartz coverslips to users in the
microscopy community for some number of years. Quartz coverslips are also
offered by several other of the leading suppliers of consumables to the
microscopy market.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Thu Apr 20 17:36:24 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Thu, 20 Apr 2000 08:58:00 -0700 (PDT)
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please unsubscribe.



From daemon Thu Apr 20 17:36:26 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 20 Apr 2000 14:27:04 -0400
Subject: Re: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christian and colleagues:

I take it you are referring to Link AN10000, QX2000 or eX/L spectra. There
are a number of ways to analyze these spectra post facto. All depend on
having them on a DOS disk, which presumably you do. If you have them on
LINK formatted floppys, then you can read them on a DOS machine with a
small program RDL2.EXE which I wrote a while ago, although that program
will not read subdirectories on LINK floppys. It copies the files
byte-by-byte to a DOS hard drive. Run the program to get three lines of
useage instructions.
Once they are in DOS, you can do a number of things:

1) Use my program LKSPCV.EXE which will convert them into a text file
formatted for direct input into Excel or another spreadsheet or graphing
program. LKSPCV knows about the differences between eX/L files and
AN10/QX2000 files, and I believe (though I've never tested) that it will
also handle the old LINK 860 files correctly, too. Usage: LKPSCV
infilename.sp outfilename.txt

2) Use the program DTSA (Desktop Spectrum Analyser) which used to be sold
by NIST. I know that the status of that program has changed, and I don't
know if it is still available or not. However, it runs on a Mac, and can
import both AN10/QX2000 and eX/L files, as well as MSA formatted files.

3) If you have access to a LINK ISIS analyser via a friend or colleague,
these systems can import and process eX/L or AN10/QX2000 spectrum files.

LKSPCV.EXE and RDL2.EXE are available by FTP from prism.mit.edu:2101. Ther
are a number of other files on that site, too, of which the useful ones are
LKCONV.COM which formats LINK disks on a PC (which must be running plain
DOS - not a DOS window) and LKCV3.EXE which extracts individual images and
maps from LINK eX/L or AN10/QX2000 studies.


At 10:21 AM 04/20/2000 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Thu Apr 20 17:36:30 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 20 Apr 2000 18:06:06 -0400
Subject: RE: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a program that I wrote in Visual Basic 6 that will read the EMSA
format EDS and EELS data, plot them, color, them, overlay them, print them,
copies the graphical data to an RTF document, and can convert them to the
EMSA format with two column X,Y pairs. I have been toying with the idea of
selling the program through South Bay Technology inexpensively. I have no
idea what the market would be for something like this. I have given a few
copies of it out for evaluation and hoped to get feedback about what I
should add to it, but no one has gotten back to me. The program is
primarily geared for EELS spectra but works with EMIspec, Noran, and DTSA
EMSA formatted EDS spectra. If you agree to give me feedback on the program
(and a pitcher of beer at M&M 2000 if you go), I might be able to be
convinced to send you a copy. I would like to know if it works with some of
the other EDS systems.

The reason that I wrote it was that the Gatan EL/P program output the EMSA
format in y-only format with columns of five. Gatan isn't the only company
to do that, I believe that the Noran data comes out that way. That format
is difficult to parse properly in Excel so that you can graph it. What I
did was to open the ASCII data in a word processor, replace all the hard
returns with a comma, then replace all the commas with a hard return, and
then resave as an ASCII text file. Then you can open it in (or copy and
paste the data) in Excel. Then you move the column over once and create the
X values for plotting the data as X,Y pairs.

I originally wrote the program to just convert it to X,Y pairs so that I
could use Excel but then it was so easy to graph it in VB6, that I just got
carried away.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--


} -----Original Message-----
} From: Filion, Christian [mailto:christian.filion-at-atlasstainless.com]
} Sent: Thursday, April 20, 2000 10:21 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: EDS spectra on PC
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Good morning,
}
} I have both a Spectra file in Link format and txt format
} saved as a MSA
} type file. Is there a software or an excel macro that could read these
} spectra ?
}
} Thank you
}
} Christian Filion
} Superviseur Laboratoires
} Aciers Inoxydables Atlas
} 1640 Marie-Victorin
} Tracy, QuŽbec
} J3R 5R5
} TelÊ: 450-746-5243
} faxÊ: 450-746-5241
}



From daemon Thu Apr 20 21:43:49 2000



From: Robert.Carlton-at-aventis.com
Date: Thu, 20 Apr 2000 17:37:42 -0500
Subject: Duane Hunt Limit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

I am trying to track down information on the short wavelength cut-off used
in EDS microanalysis to determine the true electron accelerating voltage. I
have a number of articles describing its determination, but little in the
way of historical information. It is also known as the 'Duane-Hunt' limit.
I cannot find any references to a Duane or a Hunt. Does anyone know the
originator of the concept and why it is known as this?


Thanks
Robert A. Carlton
Aventis Pharmaceuticals
Tel 610-454-3949
Robert.Carlton-at-aventis.com




From daemon Fri Apr 21 08:28:21 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Thu, 20 Apr 2000 20:51:30 -0700 (PDT)
Subject: x-ray microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking to upgrade our EDS system with a 4pi based system or Evax.
Does any one have any opinions about these systems or is there others you
could recomend?
The TEM is a CM20 with an EDAX detector.



#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Thu Apr 20 17:36:25 2000



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Thu, 20 Apr 2000 11:36:36 -0500
Subject: Re: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christian

The MSA text format is written in such a way so that the data should be
directly importable into any standard spreadsheet program.

Just import it as ASCII text with a "comma" deliminator between columns.

I am presuming that you stored the data in 2 column (X,Y) format.
If you did otherwise you will need to do a small amount of cleanup
but it should not be difficult. The other obvious trick is to go
back to the EDS system and make sure you store the data in X,Y
single column format;

Nestor



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


==================================================================
Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..." so I bought
a G3 Mac

==================================================================




From daemon Fri Apr 21 17:44:05 2000



From: Rwhentges-at-aol.com
Date: Fri, 21 Apr 2000 14:48:23 EDT
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Please unsubscribe.



From daemon Fri Apr 21 17:44:06 2000



From: Ed Vicenzi :      vicenzi-at-volcano.si.edu
Date: Fri, 21 Apr 2000 16:22:12 -0400
Subject: Smithsonian SEM Lab Manager

Contents Retrieved from Microscopy Listserver Archives
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The SEM lab manager position at the Smithsonian Institution is now open.

The National Museum of Natural History in Washington, DC is seeking an
experienced electron microscopist to fill a vacancy for SEM laboratory
operation and management. The SEM facility is designed to serve both the
biological and geological research communities in the museum, and houses
two recent model SEMs and one state-of-the-art environmental microscope (to
be installed in mid-2000). The principal responsibilities include training
staff members and visiting scientists in proper use of equipment and theory
of electron generation and detection, maintenance and troubleshooting all
instrumentation (in conjunction with full service contracts), evaluation of
new developments in SEM technology, and supervision of a support staff
member. The successful applicant will also have the opportunity to gain
experience in Focused Ion Beam (FIB) Microscopy and high spatial resolution
secondary ion mass spectrometry HR SIMS.

This position will fill a federal government vacancy and is offered at the
GS11 ($42,724) or GS12 ($51,204) levels, and carries promotion potential to
grade GS 13. U.S. citizenship is required for this federal position. To
obtain information concerning this vacancy call our automated Jobline at
(202) 287-3102 (24 hours, 7 days a week), press 9 and request vacancy
announcement 00MQ-2069, then follow voice prompts to have information faxed
or mailed to you. The application deadline is May 16th, 2000. If
questions arise after receiving and reading through the vacancy
announcement please contact: Dr. Edward Vicenzi (Chair, Search Committee)
at vicenzi-at-volcano.si.edu. The Smithsonian Institution is an equal
opportunity employer.


NOTE: Completed Smithsonian applications must be received by May 16th, 2000.




~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Edward P. Vicenzi
Smithsonian Institution
Department of Mineral Sciences
Washington, DC 20560-0119

(202) 357-2594
(202) 357-2476 (fax)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Apr 23 09:07:39 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Sat, 22 Apr 2000 13:31:10 -0400
Subject: RE: Short-Wavelength limit

Contents Retrieved from Microscopy Listserver Archives
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The short wavelength limit for x-ray spectra produced by excitation with an
electron beam is discussed and illustrated in the section on "The
Continuous Spectrum" (I believe Sect. 1.3) in Chapter 1 of The book
'Elements of X-ray Diffraction', by B. D. Cullity, Addison Wesley.

Basically, you are dealing here with the Bremsstrahlung radiation which is
generated by electrons in the incident electron beam which lose increments
of their energy as they interact with the specimen. Each incremental
energy loss gives rise to an x-ray photon having a photon energy equal to
the energy loss increment, i.e. Energy loss of a beam electron = Energy of
generated Bremsstrahlung photon. The maximum amount of energy a beam
electron electron can give up occurs when it is stopped in a single
collision, whereupon the energy loss equals the energy imparted to the
electron by the applied accelerating voltage, and is numerically the same
as the value of the accelerating voltage when expressed in keV. (i.e. an
accelerating voltage of 20 kV gives electrons a kinetic energy of 20 keV) A
maximum energy loss event of this kind produces a Bremsstrahlund photon
with the highest possible energy. Photon wavelengths decrease as photon
energies increase, and so this Bremsstrahlung photon of maximum energy also
is the photon with the shortest wavelength. Ergo, if you measure the
photon energy at the short wavelength limit, you know the value of the
accelerating voltage.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Mon Apr 24 07:47:50 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 24 Apr 2000 21:56:25 +1000
Subject: RE: Quartz coverslips purity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very few people use quartz coverslips, but they should not be afraid. I
understand that the only USA manufacturer is General Electric and almost
certainly all pure quartz products made in USA are made from quartz supplied by
GE. Our quartz slips and slides are made from GE quartz too.
I think that it is important for endusers to know when supplies are in fact
standard (however excellent) or "extra special".
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, April 21, 2000 1:51 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com]
wrote:
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Steven Ridge wrote:
} ==========================================================
} Can someone help me with a US source to purchase, low fluorescence quartz
} coverlips for conventional microscope slides. Please respond directly at
} the following email address.
} ===========================================================
}
} . . . .The optical characteristics of (fused) quartz are very very sensitive
to the
} impurity levels present. Therefore it is important that one use coverslip
} that are of reproducible quality and of known impurity levels (also linked
} from above webpage). The particular quartz used for the manufacture of the
} SPI quartz coverslips is the same "electronic grade" used in many
} applications in the electronics industry. The coverslips and quartz are of
} USA manufacture (just in case one might wonder about origin).
}
} Disclaimer: SPI Supplies has supplied quartz coverslips to users in the
} microscopy community for some number of years. Quartz coverslips are also
} offered by several other of the leading suppliers of consumables to the
} microscopy market.
}
} Chuck



From daemon Mon Apr 24 18:23:28 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 24 Apr 2000 11:01:14 -0500
Subject: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
This is off the microscopy subject...sorry.
Any recommendations on digital cameras for copy stand work and general
photography? Has anyone tried the Nikon Coolpix 990?
We're coming up on the end of the fiscal year and there might be funds
available for a digital camera so any advice is greatly appreciated.
thanks,
Beth Richardson

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************




From daemon Mon Apr 24 18:23:28 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Mon, 24 Apr 2000 11:01:21 -0400
Subject: Re: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
The status is now that DTSA is being given away free by NIST, go to
http://www.cstl.nist.gov/div837/837.02/dtsa.html
it is Macintosh only but imports many file types.
Scott

} Anthony Garratt-Reed wrote:
..snip...
} 2) Use the program DTSA (Desktop Spectrum Analyser) which used to be sold
} by NIST. I know that the status of that program has changed, and I don't
} know if it is still available or not. However, it runs on a Mac, and can
} import both AN10/QX2000 and eX/L files, as well as MSA formatted files.
..snip...


------------------- note: new mailing address ------------------------
Scott Wight fax: 301-417-1321
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | email: scott.wight-at-nist.gov
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Mon Apr 24 18:23:30 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Mon, 24 Apr 2000 11:27:41 -0400
Subject: NESM Spring Symposium-Woods Hole

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The 17th Annual New England Society for Microscopy (NESM) at Woods Hole, MA
will be held Friday,
May 12th and Saturday, May l3th.

Highlights of the meeting include: Invited Presentations in both the
Biological and Physical Sciences
arena, Commercial exhibits, Poster/Photomicrograph Award Contest, Banquet &
Cocktail Get-Together,
Discovery Cruise, Door Prizes, and much more!

For information re: registration, meeting agenda, poster/photomicrograph
submission forms, accomodations, and directions, please contact Peggy
Sherwood, Corresponding Secretary (NESM) at
MESnesm-at-aol.com. A newsletter will be mailed to you. Please respond
ASAP-registration deadline is
Monday, May 1, 2000!

Hope to see you at Woods Hole!

Peggy Sherwood
MESnesm-at-aol.com




From daemon Mon Apr 24 18:23:32 2000



From: RonMervis-at-aol.com
Date: Mon, 24 Apr 2000 13:11:07 EDT
Subject: kodak digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi all....
we are considering getting a kodak MDS 120 digital camera for photomicroscopy
(brightfield)...does anyone have any experience with this system...is it cost
effective....? user friendly?
Is there any other system that would be recommended?
thanks for any feedback
Ronald F. Mervis, Ph.D.
RonMervis-at-aol.com
~~~~~~~~~~~~~~~~
Neuro-Cognitive Research Laboratories
2109 West Fifth Ave
Columbus, OH 43212



From daemon Mon Apr 24 18:23:33 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 24 Apr 2000 12:38:28 -0500
Subject: RE: Xray

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Long time ago I had worked in a laboratory which had
a linear electron accelerator. It was well known that
regular glasses (made from glass, not plastic) left
for a few days close to accelerator would turn in a
pretty good sunglasses. Effect would last for a few
month, and then glasses again would be clear. So,
every spring a few glasses were sitting close to accelerator.
Sure, energy was much higher than for SEM.

Vladimir Dusevich

} Dear Joe and list members:
}
} I did not say anything about yellowing of ceramics at the XEI
} Scientific web
} site. It is generally not a "contamination" problem. X-rays being an
} ionizing radiation are able to break bond and modify the crystalline
} structure of ceramics. When I did X-ray spectrometry I was
} very aware of the
} ability of high intensity, high energy x-rays to cause
} radiation damage and
} discoloration. However scanning electron microscope are
} generally operated
} at low energies ( {30KeV) and low beam currents. Xray damage
} is not a problem
} except with most sensitive materials.
}
} Xray damage to oils could cause them to crosslink and
} yellow if oils are
} deposited on the surface of ceramic but I have not seen any
} complaints about
} this. Maybe a more specific description of your problem would
} help. Reply
} off list.
}
} Notice: XEI Scientific sells anti-contamination systems.
}
} Ronald Vane
} XEI Scientific
}
} -----Original Message-----
} } From: Joe {jmetzger-at-suscom.net}
} To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} Date: Wednesday, April 19, 2000 5:59 PM
} Subject: Xray
}
}
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} } -------------------------------------------------------------
} ----------.
} }
} }
} } I recently completed a research paper to complete my EE
} } Degree which included a section on vacuum principles. I
} } incorporated part of the in formation I got from an article
} } by XEI scientific on contamination control. It spoke of oil
} } deposits on cool windows or a loss of low energy xray. I
} } thought I also read (on the web site) of how x-rays will
} } turn ceramics yellow. I can not find the article again.
} } Can you help me with this? What I am trying to decipher is,
} } will concentrated xray emission through a ceramic cause it
} } to yellow and could contamination deposits be the cause of
} } this? Would appreciate any answers or leads as to where I
} } might find the answer.
} }
} } Sincerely
} } Joe Metzger
} }
} }
} }
} }
}
}



From daemon Mon Apr 24 18:23:45 2000



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Mon, 24 Apr 2000 14:59:22 -0400
Subject: Immunogold labeling of platelets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'll be attempting to do some post-embedding immunogold labeling of murine
platelets. I'm soliciting advice on preferred fixatives and resins. I'd
like to stay away from cryosectioning if possible. If anyone has a great
technique or special tips, I'd like to hear about it.

Thanks in advance!


Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Mon Apr 24 18:23:45 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Mon, 24 Apr 2000 14:56:47 -0400
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
4700) utilizing their new octagonal pixel technology (Super CCD) and it
sells for {$1000. I saw it in a catalog from Publishing Perfection
(http:/www.publishingperfection.com)

At 11:01 AM 4/24/00 -0500, Beth Richardson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Apr 24 18:23:45 2000



From: Ken Bart :      kbart-at-hamilton.edu
Date: Mon, 24 Apr 2000 15:16:06 -0700
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Beth:
There is an excellent review of the Nikon 990 at
http://www.dpreview.com/reviews/nikoncp990/. Maybe this addresses
your question. I suspect that this camera would be somewhat limited
for copy stand work. I do a lot of digital imaging in my lab and use
a Nikon D1, but this is considerably more expensive then a Coolpix
990. A good alternative is the Leaf Lumina which is marketed by
Electron Microscopy Sciences and others. I hope this helps!!!!!!

Ken Bart

Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323
----------------------------------------
Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu



From daemon Mon Apr 24 18:23:46 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 24 Apr 2000 16:18:16 -0400
Subject: MME Research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To Product, Sales, and Marketing managers,

MME's Market Research survey has been extended one day in honor of Friday's
holiday. If you have not returned your survey forms, please do so by end
of business Tuesday.

If, for some reason, we missed you, please email me immediately with your
fax number.

We already have nearly 10% return. Both a summary of our findings and the
winner of the M&M '99 Master Report will be posted Wednesday on
www.MicroscopyMarket.com

Thanks for your participation.

Barbara Foster,President
Microscopy/Marketing & Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/marketing

MME is a full service technical marketing company specializing in
microscopy and related imaging technologies.
Catalytic information and more .... We help your company grow!
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%



From daemon Mon Apr 24 18:23:47 2000



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Mon, 24 Apr 2000 15:34:08 -0500
Subject: RE: Quartz coverslips purity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

GE does sell quartz glass in the US. I don't recall seeing coverslips, but
we have purchase quartz that was approximately 1 mm in thickness. Here is
their contact information and a
website.

4901 Campbell Road
Willoughby, Ohio 44094 USA
Phone: (216) 266-3590 or 1-800-438-2100
FAX: (216) 266-4043 or 1-800-258-3803

http://www.gespectrum.com/inet/quartz/english/
Email: quartz-at-lighting.ge.com


Raj

**********************************************
Dr. Raj Lartius, CEO
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Voice: 515-795-3164
Fax: 515-795-4414
Web: www.novascan.com
**********************************************
"Innovative Tools to Explore the Microworld"



From daemon Mon Apr 24 18:23:49 2000



From: Doug Cromey :      doug-cromey-at-ns.arizona.edu
Date: Mon, 24 Apr 2000 14:53:55 -0700
Subject: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

I was asked today if there exists a book that broadly covers all of the
different types of Microscopy (LM, EM, Probe Microscopes, etc) and gives
their pros & cons and perhaps examples of their use. The faculty member
who made the request is not a trained microscopist, so they would prefer
something more general.

If there is such a book, it sounds like it would be an interesting one to read.

Yours,
Doug Cromey
....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Mon Apr 24 18:23:49 2000



From: William Snell :      william.snell-at-email.swmed.edu
Date: Mon, 24 Apr 2000 16:53:38 -0500
Subject: LM: looking for used Zeiss DR-C stereomicroscope or equivalent

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a stereomicroscope with Greenough optics that provides
final magnification of ~10X to 64X or 80X. I want to use it for
visualizing algal cells growing on agar petri dishes. The cells are
about 5 microns in diameter and I want to push them around on the agar
using fine glass needles. I understand that the old Zeiss DR-C works
well for this application. Vermont Optech and Sciscope have been very
helpful and may have usable, used systems. Any other suggestions/ideas
are welcome.

William J. Snell, Ph.D.
University of Texas Southwestern Medical Center
T-214-648-2332
F-214-648-8694
email-William.Snell-at-email.swmed.edu




From daemon Tue Apr 25 22:54:32 2000



From: De Pauw Bart :      bart.depauw-at-rug.ac.be
Date: Tue, 25 Apr 2000 15:32:15 +0200
Subject: Holder Filter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I am searching some device for holding (polycarbonate)filters during
dehydration and CPD. I am trying to view mycoplasms with SEM. Has anyone
experience with this ?

Greetings,


De Pauw Bart
Ghent University
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90



From daemon Tue Apr 25 22:54:33 2000



From: spb :      spb-at-mwrn.com
Date: Tue, 25 Apr 2000 09:28:18 -0400
Subject: Help locating Live Cell Analysis in Calif or AZ

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you can help with the message below, please respond to
BarbaraRBC-at-aol.com [mailto:BarbaraRBC-at-aol.com]

Please do NOT respond to the list

Susanne P Brandom
MicroWorld


MESSAGE

Can you assist by providing a list of live cell blood testers in the
California/Arizona area? If not, are you able to point me in the direction
where I might find a list of microscopists who perform this type of test?

Thank you

Barbara Churchill
(909) 624-2459 ( fax and voice mail)



From daemon Tue Apr 25 22:54:33 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 25 Apr 2000 09:26:47 -0400
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: Bill Miller [mailto:microbill-at-mohawk.net]
} Sent: Monday, April 24, 2000 2:57 PM
} To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} Subject: Re: digital cameras for copy stand work?

} Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} sells for {$1000. I saw it in a catalog from Publishing Perfection }


Fuji has two new cameras coming out based on their new "Super CCD"
technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
imaging, an important issue is image interpolation and you must be aware of
how Fuji determines resolution of these new cameras.
The Super CCD technology utilizes octagonal pixels which are aligned
at an angle, as opposed to horizontal rows for a conventional CCD with
square
pixels. The advantages of this design include denser packing of pixels, and
having pixels sensitive to R,G,B in each row. It is a very interesting
technology
to say the least.
Look closely at any Fuji literature about these cameras. The Finepix 4700
has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
resolution
of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
resolution
of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
million
pixels.
Fuji's image size specs are based on their claim that the image quality
with the
S1 Pro will be equivalent to an image captured with a 6 million pixel
"conventional" CCD
camera, or a 4.3 million pixel conventional CCD camera with the 4700.
I have no doubt that the new cameras will produce high quality images. I
can
also say for sure that the cost of the new cameras is very affordable when
compared to
similar products currently available. It remains to be seen what effects
that Fuji's
interpolation will have on image integrity.
S-1 Cameras are expected in late May or early June, I will be happy to
provide
sample images to any who request them at that time. Finepix 4700 cameras are
currently
starting to ship from Fuji.

George Laing
National Graphic Supply
scisales-at-ngscorp.com
(800) 223-7130 X3109



From daemon Tue Apr 25 22:54:34 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 25 Apr 2000 10:06:47 -0400
Subject: Re: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Doug,

Our book, "Optimizing Light Microscopy" covers the light end of things and
there is a short chapter covering EM, confocal, etc. Details are on our
website: MME-Microscopy.com/education. A number of colleges and
universities have started using it as a text, most recently, U Wash (Kip
Hauch).

Caveat: MME does have a financial interest in this project.

Best regards
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^


At 02:53 PM 4/24/00 -0700, Doug Cromey wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Apr 25 22:54:36 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Tue, 25 Apr 2000 10:33:24 -0400
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks George!

I was tempted to write a similiar response, but it would seem too biased. However, I
just read a recent Wall Street Journal article that stated that Olympus Corporation
has filed a law suit against Fuji. Appearantly, Fuji does not feel they have a strong
defense as they are instructing Dealers to place a correction sticker over the
resolution specifications on the boxes and make disclaimers in all advertisements.

Regards,

Lawrence Kordon
Nikon, Inc.
nikon-at-jagunet.com

George Laing wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } From: Bill Miller [mailto:microbill-at-mohawk.net]
} } Sent: Monday, April 24, 2000 2:57 PM
} } To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} } Subject: Re: digital cameras for copy stand work?
}
} } Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} } 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} } sells for {$1000. I saw it in a catalog from Publishing Perfection }
}
} Fuji has two new cameras coming out based on their new "Super CCD"
} technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
} imaging, an important issue is image interpolation and you must be aware of
} how Fuji determines resolution of these new cameras.
} The Super CCD technology utilizes octagonal pixels which are aligned
} at an angle, as opposed to horizontal rows for a conventional CCD with
} square
} pixels. The advantages of this design include denser packing of pixels, and
} having pixels sensitive to R,G,B in each row. It is a very interesting
} technology
} to say the least.
} Look closely at any Fuji literature about these cameras. The Finepix 4700
} has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
} resolution
} of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
} resolution
} of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
} million
} pixels.
} Fuji's image size specs are based on their claim that the image quality
} with the
} S1 Pro will be equivalent to an image captured with a 6 million pixel
} "conventional" CCD
} camera, or a 4.3 million pixel conventional CCD camera with the 4700.
} I have no doubt that the new cameras will produce high quality images. I
} can
} also say for sure that the cost of the new cameras is very affordable when
} compared to
} similar products currently available. It remains to be seen what effects
} that Fuji's
} interpolation will have on image integrity.
} S-1 Cameras are expected in late May or early June, I will be happy to
} provide
} sample images to any who request them at that time. Finepix 4700 cameras are
} currently
} starting to ship from Fuji.
}
} George Laing
} National Graphic Supply
} scisales-at-ngscorp.com
} (800) 223-7130 X3109



From daemon Tue Apr 25 22:54:37 2000



From: RCHIOVETTI-at-aol.com
Date: Tue, 25 Apr 2000 11:48:19 EDT
Subject: Re: kodak digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 04/24/2000 10:32:32 AM US Mountain Standard Time,
RonMervis-at-aol.com-at-sparc5.microscopy.com writes:

{ { we are considering getting a kodak MDS 120 digital camera for
photomicroscopy
(brightfield)...does anyone have any experience with this system...is it
cost
effective....? user friendly?
} }

Hi Ron,

The Kodak MDS 120 seems to do a decent job. Part of the MDS (Microscopy
Documentation System) with the camera is a c-mount adapter that you mount to
the phototube of your microscope. It requires a 1.0X adapter in the
phototube, and the adapter should have a c-mount on it. The Kodak adapter
then mounts on the end of the 1.0X adapter.

You may encounter some vignetting with the camera, especially if you try to
use the wide-angle setting on the camera's zoom lens. The vignetting seems
to be a function of the photo adapters which are used, and Kodak has a
disclaimer about this in the instruction manual. It may or may not be a
problem with your scope/phototube/c-mount adapter.

Part of the package which we got is a 16 MB Flash Memory card for the camera,
so you can either run the camera from your computer or store images on the
memory card and then remove the card and take it to a computer to transfer
the images to the computer. The package also had a PCMCIA card adapter in
it. The memory card plugs into the end of the PCMCIA device, and you can
then slip it into a PCMCIA slot on a laptop computer.

But if you need to use a desktop computer and don't have a PCMCIA slot, you
need to get something like a SanDisk card reader for the computer. They're
available at CompUSA, Fry's Electronics, OfficeMax, etc. for about $70.

The software with the camera is very nice. It lets you transfer images from
the camera's memory directly or from from the memory card, preview images and
download them directly from the camera, etc. as well as perform some basic
image adjustments (flip, rotate, modify brightness, contrast, gamma, etc.)

Overall, I would say the Kodak system is a nice way to document general
microscopy images in brightfield and phase contrast microscopy for under $2K.
We haven't tried to use the camera for fluorescence imaging, so I have no
history to contribute on this subject.

Hope this is of some help!

Bob Chiovetti
GTI Microsystems



From daemon Tue Apr 25 22:54:41 2000



From: Vladimir Oleshko :      Vladimir.Oleshko-at-asu.edu
Date: Mon, 24 Apr 2000 22:35:35 -0700
Subject: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is the Handbook of Microscopy: Applications in Materials Science,
Solid State Physics and Chemistry /ed by S. Amelinckx et al., v.1-3,
Weinheim, N.Y.: VCH (1997). It covers LM, EM (stationary and scanning beam
methods), X-ray and acoustic microscopy, scanning probe techniques, magnetic
and emission microscopies, image analysis, and microscopy applications to
various classes of materials as well.

Vladimir

************************************
Vladimir P. Oleshko, Ph.D.
Industrial Associates Program
Center for Solid State Science
Arizona State University
Main Campus, PO Box 871704
Tempe, AZ 85287-1704
(480) 727-7666
Fax: (480) 965-9004
E-Mail:oleshko-at-imap3.asu.edu
*************************************


-----Original Message-----
} From: Doug Cromey [mailto:doug-cromey-at-ns.arizona.edu]
Sent: Monday, April 24, 2000 2:54 PM
To: microscopy-at-sparc5.microscopy.com


Colleagues,

I was asked today if there exists a book that broadly covers all of the
different types of Microscopy (LM, EM, Probe Microscopes, etc) and gives
their pros & cons and perhaps examples of their use. The faculty member
who made the request is not a trained microscopist, so they would prefer
something more general.

If there is such a book, it sounds like it would be an interesting one to
read.

Yours,
Doug Cromey
...................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"




From daemon Tue Apr 25 22:54:50 2000



From: Shane Collins :      kshanec-at-gte.net
Date: Tue, 25 Apr 2000 15:59:26 -0700
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In addition to what George and Bill have pointed out, Fuji claims the imager
(ccd) is roughly the size of APS film on the S1 Pro. What I cannot find in
the literature is the pixel size specification whereby one can calculate or
compare the field of view (on a microscope) of this camera with other mega
pixel cameras.
Shane

K. Shane Collins
Scientific Instrument Company
805.444.4953 cell
310.568.9188 office
310.568.9189 fax


-----Original Message-----
} From: George Laing [mailto:scisales-at-ngscorp.com]
Sent: Tuesday, April 25, 2000 6:27 AM
To: Microscopy-at-sparc5.microscopy.com


} From: Bill Miller [mailto:microbill-at-mohawk.net]
} Sent: Monday, April 24, 2000 2:57 PM
} To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} Subject: Re: digital cameras for copy stand work?

} Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} sells for {$1000. I saw it in a catalog from Publishing Perfection }


Fuji has two new cameras coming out based on their new "Super CCD"
technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
imaging, an important issue is image interpolation and you must be aware of
how Fuji determines resolution of these new cameras.
The Super CCD technology utilizes octagonal pixels which are aligned
at an angle, as opposed to horizontal rows for a conventional CCD with
square
pixels. The advantages of this design include denser packing of pixels, and
having pixels sensitive to R,G,B in each row. It is a very interesting
technology
to say the least.
Look closely at any Fuji literature about these cameras. The Finepix 4700
has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
resolution
of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
resolution
of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
million
pixels.
Fuji's image size specs are based on their claim that the image quality
with the
S1 Pro will be equivalent to an image captured with a 6 million pixel
"conventional" CCD
camera, or a 4.3 million pixel conventional CCD camera with the 4700.
I have no doubt that the new cameras will produce high quality images. I
can
also say for sure that the cost of the new cameras is very affordable when
compared to
similar products currently available. It remains to be seen what effects
that Fuji's
interpolation will have on image integrity.
S-1 Cameras are expected in late May or early June, I will be happy to
provide
sample images to any who request them at that time. Finepix 4700 cameras are
currently
starting to ship from Fuji.

George Laing
National Graphic Supply
scisales-at-ngscorp.com
(800) 223-7130 X3109




From daemon Tue Apr 25 22:54:51 2000



From: bozzola-at-siu.edu
Date: Tue, 25 Apr 2000 18:48:13 -0500
Subject: Microscopy Footage Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists:

I am forwarding this request in the hopes that someone would be able
to assist these film producers to find some footage for a museum
exhibit they are working on.

Thanks for our attention.

John Bozzola

+++++++++++++++++++++++++++++++++++++++++++++++++

Chedd-Angier is producing a series of exhibits for the Science Museum of
Virginia in Richmond, VA.(. http://www.chedd-angier.com.)
We currently produce the Scientific American Frontiers Program on
PBS.(http://www.pbs.org/saf)

Our science museum project encompasses a wide variety of exhibits
ranging from a voyage through a cell called cell watcher, to a life size
journey through the human body called BODY PROBE.
I am looking for a variety of footage pieces to use in these exhibits.
They will all be used for North American non broadcast educational use
only in a museum that DOES not have a separate admission policy for
this exhibit.
The electron micrograph footage that I am looking for is :

For cell watcher
Mitochondria
Golgi Bodies
Cell membranes
Nucleus
Chromosomes
Vesicles
Endoplasmic Reticulum
Lysosomes
Fat cells expanding
Mitosis
White blood cells
muscle cells contracting
plant stem cells elongating
cells from a fallopian tube
amoeba
sperm cells swimming
This is a very extensive list, and if there are animation related
footage sources I can use those as well.
If you have any questions at all please contact me at 617-926-8300.
Thank you

AndrŽ Stark
Producer
Chedd-Angier
70 Coolidge Hill Rd
Watertown, MA 02472
0101617-926-8300
617-926-2710(F)

+++++++++++++++++++++++++++++++++++++++++++++++



From daemon Tue Apr 25 22:54:57 2000



From: Hazel Clothier :      HazelC-at-spc.int
Date: Wed, 26 Apr 2000 12:55:16 +1100
Subject: LM basic maintenance manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As a new subscriber to the listserv I would like to ask your assistance in
locating copies of any manuals or in-house guidelines for the basic care and
maintenance of light microscopes.

I am currently assisting a group of microscopists in a humid / tropical
environment to increase their ability to care for their microscopes. Care by
the user is an important issue as is the ability to repair/maintain
microscopes in-country. Whilst we can find quite a bit of information on how
to use your microscope there is less on maintenance.

In addition does anyone have any idea on where we can purchase a "two-pin
tool" for removing lenses?

Thankyou

Hazel Clothier

Regional Laboratory Scientist
Pacific Regional Vector Borne Diseases Project
Secretariat of the Pacific Community
PMB
Suva
FIJI

Tel: (679) 321154 - direct
(679) 320066 - ex 110
Fax:(679) 322714
hazelc-at-int



From daemon Tue Apr 25 23:02:11 2000



From: Aaron Wheeler :      adog3050-at-yahoo.com
Date: Tue, 25 Apr 2000 22:56:46 -0500
Subject: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

I am a beginning microscopist. I am preparing to take
some TEM photos of granules isolated from mast cells.
The technician I am working with would like to use
phosphate buffer (+ glutaraldehyde) to fix the
granules, but the literature I've read suggests using
cacodylate buffer. What do you think about the
benefits/disadvantages of phosphate vs cacodylate
buffer for these purposes?

thanks,
Aaron

__________________________________________________
Do You Yahoo!?
Send online invitations with Yahoo! Invites.
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From daemon Wed Apr 26 06:29:45 2000



From: k.denhaan-at-consunet.nl
Date: Tue, 25 Apr 2000 23:01:20 -0500
Subject: phase contrast vs interference contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Email: k.denhaan-at-consunet.nl
Name: K.den Haan
School: dutch HBS

Question: when comparing phase contrast and interference
contrast (Nomarski) the negative points a.o.(Ph)
are halo. Interference contrast also
(especially in minute organisms) has a drawback
since depending on the position of the polarizer
some part of f.i.bacteria do not show the
relief image (the shadow) to its fullest. How
do you call this phenomenon and how can it be
explained?




---------------------------------------------------------------------------




From daemon Wed Apr 26 06:29:45 2000



From: COURYHOUSE-at-aol.com
Date: Wed, 26 Apr 2000 00:02:52 EDT
Subject: Re: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


if anyone has a used set of this avail let me know. also looking for
encyclopedia of microscopy by gray (editor)
thanks Ed Sharpe

{ { Subj: RE: General Microscopy book recommendation?
Date: 4/25/00 2:29:52 PM US Mountain Standard Time
From: Vladimir.Oleshko-at-asu.edu (Vladimir Oleshko)
Reply-to: {A HREF="mailto:oleshko-at-imap3.asu.edu"} oleshko-at-imap3.asu.edu {/A}
To: doug-cromey-at-ns.arizona.edu ('Doug Cromey')
CC: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


There is the Handbook of Microscopy: Applications in Materials Science,
Solid State Physics and Chemistry /ed by S. Amelinckx et al., v.1-3,
Weinheim, N.Y.: VCH (1997). It covers LM, EM (stationary and scanning beam
methods), X-ray and acoustic microscopy, scanning probe techniques, magnetic
and emission microscopies, image analysis, and microscopy applications to
various classes of materials as well.

Vladimir

************************************
Vladimir P. Oleshko, Ph.D.
Industrial Associates Program
Center for Solid State Science
Arizona State University
Main Campus, PO Box 871704
Tempe, AZ 85287-1704
(480) 727-7666
Fax: (480) 965-9004
E-Mail:oleshko-at-imap3.asu.edu
*************************************

} }



From daemon Wed Apr 26 06:32:08 2000



From: Lam xu Fu Christopher :      eng81067-at-nus.edu.sg
Date: Wed, 26 Apr 2000 15:13:14 +0800
Subject: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir,

I am currently using a JEOL 5800LV SEM.
My specimens are cells from cultures which we have grown in our labs.
The low vacuum condition is 7Pa.
The problem occurs when I increase the magnification, about 400 times and
beyond. The picture I get will be enlarged and clear except for certain
regions which displays some glaring effects. Playing around with the
contrasts and brightness didn't help.
I was told this was a charging problem, and increasing the vacuum might
help, so I tried. It did help a little but the picture quality was
compromised.

Are there other solutions to this problem of mine, such that the picture
quality might remain the same.

Chris Lam
BIOMAT
Mechanical & Production Department
National University of Singapore




From daemon Wed Apr 26 06:32:09 2000



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Wed, 26 Apr 2000 01:08:59 -0700 (PDT)
Subject: Books for Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following the thread from Doug Cromey asking about
books for Microscopy, I would like to suggest two
manuals for light microscopy that are comprehensive in
their coverage, *accurate in their educational
content*, and easy to read and digest.

Title: Light Microscopy, An Illustrated Guide.
Author: Ron OLDFIELD.
Location: Sydney, Australia
Published: 1994, Wolfe Publishing, imprint of
Mosby-Year Book, Europe. 160 pages
ISBN: 0-7234-1876-4
Available Amazon, no price given; ca. $20.

Title: Introduction to Light Microscopy
Authors: Savile BRADBURY & Brian BRACEGIRDLE
Location: Oxford, England
Published: 1998, Bios Scientific Publishers
Website: www.bios.co.uk
Royal Microscopical Society Handbook No. 42, 122
pages.
ISBN: 1-85996-121-5
Amazon Price: $32.95

Searching for, and finding, a suitable teaching and/or
reference text is a personal thing. It depends on your
requirements and preferences, but I suggest that these
two books will help most people in most disciplines. I
have used them for the last six years in teaching
light microscopy to a wide spectrum of students from
industry and academia.

For those intessted in digital imaging, although not a
book specifically for microscopists, I have found the
following text useful:

Title: Digital Imaging for Photographers, 3rd edn.
Authors: Adrain DAVIES & Phil FENNESSY
Published: 1998, Focal Press, Oxford, Boston.170
pages.
website: www.bh.com/focalpress
(Focal Press is an imprint of Butterworth-Heinemann)
ISBN: 0-240-51538-2
Amazon Price: $31.96

I am both a professional and an amateur microscopist,
please do not think I am denigrating the word amateur,
but as an introductory text for amateur
microscopists,or those starting out in a complex
field, I would suggest:

Title: Exploring With the Microscope : A Book of
Discovery & Learning
Author: Werner NACHTIGALL
Publisher: Stirling Publishing Corp. Inc., April 1997.
160 pages
ISBN: 0-80690866-1 (check that this is 2nd edition)
Amazon Price: $11.96

Finally, if you consider that getting school children
interested in microscopy, and science generally, is an
important investment for the future, then I would
suggest the new Usborne Complete Book of the
Microscope, which shows the relevance and the
application of microscopy in many walks of life.

Title: The Usborne Complete Book of the Microscope
Author: Kirsteen ROGERS
Publisher: 1998, Usborne Publishing Ltd, 96 pages.
website: www.usborne.com
ISBN: 0-7460-3106-8
Amazon Price: $22.95

Whatever your field, light microscopy underpins, and
is a necessary foundation for, electron microscopy,
confocal and other techniques. I hope that these
suggestions are useful. I would welcome anyone's views
to me directly.

Jeremy Sanderson
jb_sanderson-at-yahoo.com


__________________________________________________
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Send online invitations with Yahoo! Invites.
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From daemon Wed Apr 26 06:32:09 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Wed, 26 Apr 2000 10:55:50 +0200
Subject: TEM: permanganate etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I have a few PE specimens on my desk that I want to prepare with the
"permanganate etching method". Does anybody of you have some experience on
this techique? Would you share some details or tips and tricks with me on
how to obtain the best results? What is the best method to produce a
replica from the etched specimen?

Petra
--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Apr 26 06:32:10 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 26 Apr 2000 11:21:30 +0100 (GMT Daylight Time)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phosphate buffers can give rise to a precipitate with
uranyl acetate if it is used as an en block stain.
Cacodylate does not.

Cacodylate contains arsenic and must be used with care.

Dave


On Tue, 25 Apr 2000 22:56:46 -0500 Aaron Wheeler
{adog3050-at-yahoo.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} I am a beginning microscopist. I am preparing to take
} some TEM photos of granules isolated from mast cells.
} The technician I am working with would like to use
} phosphate buffer (+ glutaraldehyde) to fix the
} granules, but the literature I've read suggests using
} cacodylate buffer. What do you think about the
} benefits/disadvantages of phosphate vs cacodylate
} buffer for these purposes?
}
} thanks,
} Aaron
}
} __________________________________________________
} Do You Yahoo!?
} Send online invitations with Yahoo! Invites.
} http://invites.yahoo.com
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Apr 26 06:46:22 2000



From: Duncan Waddell :      D.Waddell-at-mailbox.uq.edu.au
Date: Wed, 26 Apr 2000 06:33:26 -0500
Subject: EM Messageboard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi folks,

The CMM at The University of Queensland (Australia) has initiated an
Internet messageboard for Microscopy and Microanalysis.

http://www.coolboard.com/boardshow.cfm?mb=545625259139077

Other EM websites around the world are invited to add this forum to
their own sites - just follow the link on the Forum to customize your
own version for your own web pages.

The service is hosted by Coolboard and is totally free.

It is currently being moderated ONLY for the purpose of keeping rubbish
and spam out of it. If all goes well moderation will be dropped.

Check it out and participate in this EM community venture if you feel it
will be of benefit.

Regards,

Duncan.

--
************************************************************
Duncan Waddell (BSc)
Senior Scientific Officer
Centre for Microscopy and Microanalysis
The University of Queensland, St. Lucia, Qld, 4072 Australia
Telephone: +61-7-3365-4216
Facsimile: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/nanohome.html
************************************************************
Any opinion expressed is that of the writer,
and not necessarily that of CMM or of the University.
************************************************************




From daemon Wed Apr 26 06:59:44 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 25 Apr 2000 21:48:32 -0400
Subject: RE: Cameras for Copy Stand use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FYI all,

A review of the Fuji Finepix 4700 is available at:
http://www.dpreview.com/reviews/fuji4700z/

A review of the Nikon Coolpix 990 is available at:
http://www.imaging-resource.com/PRODS/C990/C99A.HTM

If anyone would like literature, or sample prints/ files from the Nikon
please contact me directly.

George Laing

National Graphic Supply
226 North Allen Street
Albany, NY 12206
E-mail: scisales-at-ngscorp.com
(800) 223-7130 X3109 Phone
(800) 832-2205 Fax





From daemon Thu Apr 27 11:02:14 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 26 Apr 2000 22:44:05 +1000
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shane Collins - do you mean pixel size and numbers when you refer to field of
view?
Field of view, as I understand it has nothing to do with image quality, but is
simply the physical size covered by a photographic system on the microscope.
The crucial factors affecting field of view are objective lens mag. and the
relation between photo eyepiece and the physical size of the film or CCD. 35mm
film combined with a 3x photo eyepiece is about "normal", small CCD's require a
much lower mag eyepiece, otherwise the system gives too greater magnifications,
frequently beyond OM limits.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, April 26, 2000 8:59 AM, Shane Collins [SMTP:kshanec-at-gte.net]
wrote:
}
}
} In addition to what George and Bill have pointed out, Fuji claims the imager
} (ccd) is roughly the size of APS film on the S1 Pro. What I cannot find in
} the literature is the pixel size specification whereby one can calculate or
} compare the field of view (on a microscope) of this camera with other mega
} pixel cameras.
} Shane
}
} K. Shane Collins
} Scientific Instrument Company
} 805.444.4953 cell
} 310.568.9188 office
} 310.568.9189 fax
}
}
} -----Original Message-----
} } From: George Laing [mailto:scisales-at-ngscorp.com]
} Sent: Tuesday, April 25, 2000 6:27 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: digital cameras for copy stand work?
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } From: Bill Miller [mailto:microbill-at-mohawk.net]
} } Sent: Monday, April 24, 2000 2:57 PM
} } To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} } Subject: Re: digital cameras for copy stand work?
}
} } Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} } 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} } sells for {$1000. I saw it in a catalog from Publishing Perfection }
}
}
} Fuji has two new cameras coming out based on their new "Super CCD"
} technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
} imaging, an important issue is image interpolation and you must be aware of
} how Fuji determines resolution of these new cameras.
} The Super CCD technology utilizes octagonal pixels which are aligned
} at an angle, as opposed to horizontal rows for a conventional CCD with
} square
} pixels. The advantages of this design include denser packing of pixels, and
} having pixels sensitive to R,G,B in each row. It is a very interesting
} technology
} to say the least.
} Look closely at any Fuji literature about these cameras. The Finepix 4700
} has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
} resolution
} of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
} resolution
} of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
} million
} pixels.
} Fuji's image size specs are based on their claim that the image quality
} with the
} S1 Pro will be equivalent to an image captured with a 6 million pixel
} "conventional" CCD
} camera, or a 4.3 million pixel conventional CCD camera with the 4700.
} I have no doubt that the new cameras will produce high quality images. I
} can
} also say for sure that the cost of the new cameras is very affordable when
} compared to
} similar products currently available. It remains to be seen what effects
} that Fuji's
} interpolation will have on image integrity.
} S-1 Cameras are expected in late May or early June, I will be happy to
} provide
} sample images to any who request them at that time. Finepix 4700 cameras are
} currently
} starting to ship from Fuji.
}
} George Laing
} National Graphic Supply
} scisales-at-ngscorp.com
} (800) 223-7130 X3109
}
}



From daemon Thu Apr 27 11:02:17 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 26 Apr 2000 09:39:26 -0500
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


PO4 buffers can cause Calcium to precipitate out but the use of
cacodylate is archaic. Microscopists tend to be the most refractory
of all scientists to change. HEPES or PIPES are reasonable
alternatives for almost all procedures in which cacodylate is used -
certainly widely used for basic fixation such as you describe. they
are non-toxic, less expensive, and better buffers. good luck



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Apr 27 11:02:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 26 Apr 2000 08:33:27 -0700
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Fuji Super CCD is 23.3 x 15.6mm; 3040 x 2016 pixels.
The S1 Pro uses Nikon mount lenses and has an equivalent
35mm frame size at 1.5X the lens' focal length.

What the equivalent FOV would be on a scope is still in
question. If the S1 Pro works anywhere near how the
Nikon E1 or E2 does, I fear the Fuji camera won't be applicable
to microscopy at all. Without a lens, the camera must operate
in aperture priority mode and center weighted exposure
reading. Unless Fuji made some major changes to the basic
Nikon (N-60) body and associated electronics, they may have
just made a higher pixel count Nikon digicam.

gary g.


At 03:59 PM 4/25/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Apr 27 11:02:18 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Wed, 26 Apr 2000 16:55:34 +0100 (BST)
Subject: Re: TEM: permanganate etching

Contents Retrieved from Microscopy Listserver Archives
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Dear Petra,

Do you have the following paper to hand?

Refinement of Etching Techniques to Reveal Lamellar Profiles in
Polyethylene Banded Spherulites
Shahin,M.M., Olley,R.H., Blissett,M.J.
J. Polym. Sci. Polym. Part B: Polym. Phys. 1999, vol.37, pp. 2279-2286

This is our latest development in the technique. If you don't have a
copy, I can send you a reprint.

Generally, we use a two stage replication technique, making a first stage
replica out of cellulose acetate, then putting Ta/W shadow and carbon on
this and extracting the cellulose acetate.

If you can let me know a little bit more about your PE specimens, I can
give you a few more details specifically adapted to your type of specimen.
In particular, is it HDPE, LLDPE or LDPE?

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Thu Apr 27 11:02:22 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Wed, 26 Apr 2000 14:22:02 -0400 (EDT)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Aron,
Caco is more extractive which may (or may not) be a blessing if
you are trying to clear out the cytoplasm a bit to get some more detail.
Poshpate is more physiologic but may form artifactual granules with
glutaraldehyde and osmium (in routine tandem use). I generally use
caco for all non immuno work otherwise phosphate, pbs or hepes for
IEM. Also remember caco contains arsenic. Good luck.

Mike Delannoy
JHMI Microscopy Facility




From daemon Thu Apr 27 11:02:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 26 Apr 2000 12:52:56 -0600 (MDT)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Tue, 25 Apr 2000, Aaron Wheeler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} I am a beginning microscopist. I am preparing to take
} some TEM photos of granules isolated from mast cells.
} The technician I am working with would like to use
} phosphate buffer (+ glutaraldehyde) to fix the
} granules, but the literature I've read suggests using
} cacodylate buffer. What do you think about the
} benefits/disadvantages of phosphate vs cacodylate
} buffer for these purposes?
}
} thanks,
} Aaron
}
} __________________________________________________
} Do You Yahoo!?
} Send online invitations with Yahoo! Invites.
} http://invites.yahoo.com
}
}
}
}
Whenever possible (nearly all the time) use phosphate buffer. Cacodylate
contains arsenic which is a potent carcinogen. One should never get into
the habit of using arsenic buffer "just because". There must be a real
reason for it! Once cacodylate is in permanent use in a laboratory, dust
accumulates on the outside of bottles, on the inside of laboratory
glassware waiting to be washed, and so on. The effects are cumulative.
Arsenic is also a toxic to the kidneys, etc. Very dangerous stuff mostly
because it is in use in many laboratories day in and day out.

Hildy Crowley
University of Denver
Denver, CO



From daemon Thu Apr 27 11:02:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 26 Apr 2000 12:55:12 -0600 (MDT)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Wed, 26 Apr 2000, Patton, David wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Phosphate buffers can give rise to a precipitate with
} uranyl acetate if it is used as an en block stain.
} Cacodylate does not.
}
} Cacodylate contains arsenic and must be used with care.
}
} Dave
}
}
} On Tue, 25 Apr 2000 22:56:46 -0500 Aaron Wheeler
} {adog3050-at-yahoo.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi:
} }
} } I am a beginning microscopist. I am preparing to take
} } some TEM photos of granules isolated from mast cells.
} } The technician I am working with would like to use
} } phosphate buffer (+ glutaraldehyde) to fix the
} } granules, but the literature I've read suggests using
} } cacodylate buffer. What do you think about the
} } benefits/disadvantages of phosphate vs cacodylate
} } buffer for these purposes?
} }
} } thanks,
} } Aaron
} }
} } __________________________________________________
} } Do You Yahoo!?
} } Send online invitations with Yahoo! Invites.
} } http://invites.yahoo.com
} }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}
}
If it is necessary to use UA with a buffer, use maleate systems. No
precipitate!

Hildy Crowley
University of Denver
Denver, CO



From daemon Thu Apr 27 11:02:24 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:40 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:24 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:55 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:25 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:38:10 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking for the adjustable lucite stand marketed several years ago (at least
within this fuzzy brain of mine it was that long ago) for improving the
ergonomic interface to LMs. I have a pathologist who needs to incline his
Nikon Microphot FX-A about 5 degrees. We had just about decided to have a
fixed unit built by the carpentry shop, when I remembered the lucite
devices. Can anyone help point me in the right directions? TIA.


Roger C Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:25 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:30 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:25 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:40 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:43 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 27 Apr 2000 09:24:58 +0100 (GMT Daylight Time)
Subject: Re: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To reduce charging try reducing kV and spot size.

Dave


On Wed, 26 Apr 2000 15:13:14 +0800 Lam xu Fu Christopher
{eng81067-at-nus.edu.sg} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Sir,
}
} I am currently using a JEOL 5800LV SEM.
} My specimens are cells from cultures which we have grown in our labs.
} The low vacuum condition is 7Pa.
} The problem occurs when I increase the magnification, about 400 times and
} beyond. The picture I get will be enlarged and clear except for certain
} regions which displays some glaring effects. Playing around with the
} contrasts and brightness didn't help.
} I was told this was a charging problem, and increasing the vacuum might
} help, so I tried. It did help a little but the picture quality was
} compromised.
}
} Are there other solutions to this problem of mine, such that the picture
} quality might remain the same.
}
} Chris Lam
} BIOMAT
} Mechanical & Production Department
} National University of Singapore
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Apr 27 11:02:46 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 27 Apr 2000 04:30:45 -0700 (PDT)
Subject: Re: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Apologies for the multiple messages. The stupid excitemail server kept
telling me the message could not be sent and then deleted the message from
my inbox. So, I wrote another one, sent that, etc. Next time, I'll wait
until I see if the listserver sends the one through before resending. :-(
On Wed, 26 Apr 2000 12:32:40 -0700 (PDT),
rmoretz-at-rdg.boehringer-ingelheim.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Looking for the adjustable lucite bases made several years ago for
adjusting
} a LM for improved ergonomic access/use. I had the
} information/supplier/manufacturer info around here someplace, but other
than
} the somewhat random neuronal firing, I appear to have
} lost/misplace/misfiled/disposed of everything. I have a pathologist who
} needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
} the process of having a fixed wooden base constructed, but I much prefer
the
} lucite devices. Any help/ideas? TIA.
}
}
} Roger C Moretz, Ph.D.
}
}
}
}
}
} _______________________________________________________
} Get 100% FREE Internet Access powered by Excite
} Visit http://freelane.excite.com/freeisp
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:48 2000



From: Harmon.Stephen-at-epamail.epa.gov
Date: Thu, 27 Apr 2000 10:36:37 -0400
Subject: Re: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


|--------+-----------------------}
| | eng81067-at-nus.|
| | edu.sg |
| | |
| | 04/26/2000 |
| | 03:13 AM |
| | |
|--------+-----------------------}
} ---------------------------------------------------------|
| |
| To: Microscopy-at-sparc5.microscopy.com |
| cc: |
| Subject: SEM charging effects |
} ---------------------------------------------------------|











------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} Dear Sir,

} I am currently using a JEOL 5800LV SEM.
} My specimens are cells from cultures which we have grown in our labs.
} The low vacuum condition is 7Pa.
} The problem occurs when I increase the magnification, about 400 times and
} beyond. The picture I get will be enlarged and clear except for certain
} regions which displays some glaring effects. Playing around with the
} contrasts and brightness didn't help.
} I was told this was a charging problem, and increasing the vacuum might
} help, so I tried. It did help a little but the picture quality was
} compromised.

} Are there other solutions to this problem of mine, such that the picture
} quality might remain the same.

} Chris Lam
} BIOMAT
} Mechanical & Production Department
} National University of Singapore

Chris,

I assume that your samples are not coated. 7 Pa may be too much of a vacuum
for uncoated samples; enough gas molecules are needed to help neutralize the
charge but too many tend to attenuate the signal. I generally operate the 5800
LV at 20-27 Pa without abundat charging artefacts. I would suggess 5 kV as
maximum gun potential and a probe current of 10 or less.

Cheers,


Stephen M. Harmon
Electron Microscopist
United States Environmental Protection Agency
M.S. 681
26 W. Martin Luther King Blvd.
Cincinnati, OH 45268
513.569.7184
Harmon.Stephen-at-epa.gov






From daemon Thu Apr 27 18:56:24 2000



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Thu, 27 Apr 2000 15:00:04 -0400
Subject: N.Y.M.S. Chemical Microscopy Workshop LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society

Bernard Friedman Memorial Workshop

Chemical Microscopy
May 20 & 21, 2000

A beginning course on chemical microscopy covering the basic techniques of
microchemical analysis using a microscope

The workshop will consist a weekend of lectures and hands on labs to cover
theoretical and practical aspects of chemical microscopy. We expect to
follow up with a second weekend course at a more advanced level for which
this will be a prerequisite. The course instructors will be the well known
and respected "Skip" Palenik of Microtrace, Inc. in Elgin, IL and N.Y.M.S.
Instructor Don O'Leary.

WHEN: May 20 & 21, 2000 from 10 A.M. to 4 P.M.

WHERE: 1244 McBride Avenue, West Paterson, NJ. Phone (973) 812-8377
(Free parking, accessible by public transportation, Information on car pools
and transportation will be provided.)

COST: $475 for N.Y.M.S. members, $495 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: Those with some basic knowledge of chemistry and chemical analysis and
use of the microscope.

HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849

PLEASE POST
----------------------------------------------------------------------------
-------------------------------------
Registration Form
Chemical Microscopy

N.Y.M.S. Member_________________ ($475) Non-Member__________($495)

Name___________________________________________________________________
Address__________________________________________________________________
Phone (W)________________(H)______________________E-Mail___________________



From daemon Thu Apr 27 18:56:29 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 27 Apr 2000 15:59:53 -0500
Subject: RE: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are you sure it is a charging and not a burning?
But anyway you can try some these steps:
1. If you are using slow scan, increase frequency of scanning
and average frames.
2. Just decrease beam intencity and kV (may be you have too
nice beam) until an image become too noisy.
3. Decrease beam intencity. If you are using BSE you can try to
increase kV too. For SE (and I am not sure what type of it you
can have) try increase pressure and kV, or decrease pressure and kV.

Good luck


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Lam xu Fu Christopher [mailto:eng81067-at-nus.edu.sg]
} Sent: Wednesday, April 26, 2000 2:13 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: SEM charging effects
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear Sir,
}
} I am currently using a JEOL 5800LV SEM.
} My specimens are cells from cultures which we have grown in our labs.
} The low vacuum condition is 7Pa.
} The problem occurs when I increase the magnification, about
} 400 times and
} beyond. The picture I get will be enlarged and clear except
} for certain
} regions which displays some glaring effects. Playing around with the
} contrasts and brightness didn't help.
} I was told this was a charging problem, and increasing the
} vacuum might
} help, so I tried. It did help a little but the picture quality was
} compromised.
}
} Are there other solutions to this problem of mine, such that
} the picture
} quality might remain the same.
}
} Chris Lam
} BIOMAT
} Mechanical & Production Department
} National University of Singapore
}
}
}



From daemon Thu Apr 27 18:56:29 2000



From: Pearl Martin :      image-at-optonline.net
Date: Thu, 27 Apr 2000 16:59:37 -0400
Subject: Job: Senior Metallographic Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am a technical recruiter. Currently, I am working on a search for one
of my Northeast clients for a
Senior Metallographic Technician:


Will perform metallographic evaluation of coatings including work with
SEM (Scanning Electron
Microscopy) and X-ray. Will be responsible for the metallurgy
laboratory, including organization of
work, and operation and calibration of equipment. Will write technical
and engineering reports. Will
prepare projects for the metallurgy laboratory. Will maintain
communication with customers.

AAS/BS plus fours years experience in a metallography laboratory.

Resumes should be sent by email, mail or fax. For best results, please
send emails as attached
Word.docs - I have Word 97.

Pearl Martin
Image Associates Inc.
5254 Merrick Road
Massapequa, NY 11758
Phone (516)798-3993
Fax (516)797-8703
Email: pearl-at-jobspot.com





From daemon Thu Apr 27 19:27:13 2000



From: Michelle.Taurino-at-aventis.com
Date: Thu, 27 Apr 2000 19:04:01 -0500
Subject: Leica- EM stainer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am in the market for the Leica EM stainer. I was just looking for some
feedback (positive or negative) about this instrument...Any recommendations?

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com




From daemon Thu Apr 27 19:27:17 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 27 Apr 2000 15:34:17 -0700
Subject: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I am turning to all of you as a last ditch effort to see if my diamond
knife is crazy or I am. I have been using this same knife for years
(approximately 4 years) with the occasional difficulty. However, in the
past few weeks I have been having a very hard time getting sections. The
problem is this: water in the boat either wets the block or pulls away from
the knife edge. There seems to be no in-between. Every once in a while, the
stars seem to align, and the level achieves perfection for a few sections.
I've tried several different cleaning methods, thinking that maybe the
knife was dirty since I've been sectioning a lot of LR White. The only
thing I've noticed (since I've been looking so very closely at the diamond)
is that the seal (which was damaged when the knife arrived from the
manufacturer) between the knife and the boat has detached. I would think
that this would cause a leakage problem, which I haven't seen, not the
problem I'm experiencing.
Does anyone have any advice?
Thanks for your help,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Thu Apr 27 19:27:25 2000



From: Julian Smith III :      smithj-at-Winthrop.edu
Date: Thu, 27 Apr 2000 18:36:15 -0400
Subject: Cleaning closets....

Contents Retrieved from Microscopy Listserver Archives
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Hi, all:
We have the following that are surpus to our needs:
-Various bits of a Kevex 7000 mainframe (cards, keyboard)

-Bits of a Kevex Analyst 8000 mainframe (cards, keyboard, drives)

-Software and manuals for both of the above systems

-Fisher Code-on histomatic slide stainer (given to us as "working", but not
tested)

-EG&G Ortec system 5000. Bad power supply.

Any of the above free to non-profits for the cost of shipping.

If you're a commercial entity and would like any of the above, let's talk
trade for some of your junque... (preferably, *smaller* junque)

contact me at telephone# below or via e-mail at smithj-at-winthrop.edu

cheers,
Julian

Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)




From daemon Thu Apr 27 19:27:30 2000



From: M. Baxter :      mykkb-at-juno.com
Date: Thu, 27 Apr 2000 18:49:55 -0400
Subject: Re: SEM Charging

Contents Retrieved from Microscopy Listserver Archives
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Tilting the sample toward the detector (up to about 45 degrees) helps
eliminate some types of charging. It is easy enough and worth a try
before more involved techniques are tried.


Mike Baxter
Lehman College

________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Thu Apr 27 19:27:33 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 27 Apr 2000 19:18:43 -0500
Subject: RE: permanganate etching

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Petra:
Are you using "permanganate etch" on a metallographic specimen? If so,
Vander Voort's book "Meatallogrphy: Principals and Practice" covers the use
of permanganate etches. See also Petzow's book on etching, in German or
English.

Sam Purdy
National Steel Technical Center
Trenton, MI, USA
} ----------
} From: Petra Wahlbring
} Sent: 26, April 2000, 4:55 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM: permanganate etching
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear colleagues,
}
} I have a few PE specimens on my desk that I want to prepare with the
} "permanganate etching method". Does anybody of you have some experience on
} this techique? Would you share some details or tips and tricks with me on
} how to obtain the best results? What is the best method to produce a
} replica from the etched specimen?
}
} Petra
} --------------------------------------------------------------
} Dr. Petra Wahlbring
} Centre de Recherche Public - Gabriel Lippmann
} Laboratoire d'Analyse des Materiaux (LAM)
} 162a, av. de la Faiencerie L-1511 Luxembourg
} tel. +352-466644-402 fax +352-466644-400
} e-mail: petra.wahlbring-at-crpgl.lu
} Visit our WWW site! http://www.crpgl.lu/~wahlbrin
}




From daemon Fri Apr 28 08:06:03 2000



From: RCHIOVETTI-at-aol.com
Date: Thu, 27 Apr 2000 20:52:43 EDT
Subject: Re: diamond knife problems

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In a message dated 04/27/2000 5:27:39 PM US Mountain Standard Time,
kalen-at-citrus.ucr.edu writes:

{ { water in the boat either wets the block or pulls away from
the knife edge. There seems to be no in-between. Every once in a while, the
stars seem to align, and the level achieves perfection for a few sections.
I've tried several different cleaning methods, thinking that maybe the
knife was dirty since I've been sectioning a lot of LR White. The only
thing I've noticed (since I've been looking so very closely at the diamond)
is that the seal (which was damaged when the knife arrived from the
manufacturer) between the knife and the boat has detached. I would think
that this would cause a leakage problem, which I haven't seen, not the
problem I'm experiencing. } }

Kristen,

I had a knife that behaved similarly once upon a time. Let's assume the boat
isn't leaking, since you aren't having problems with water collecting
underneath the knife in the knife holder, correct? It would be difficult to
imagine the knife has come loose in the boat, but that may be a very slight
possibility. If it were loose in its mount it might not section with
consistency. Either way, it would be a good idea to have the knife re-sealed
properly.

The knife I had was almost impossible to get the proper amount of wetting on
the diamond, but two things helped:

1. Try deliberately overfilling the boat to give a positive meniscus right
at the knife edge. Leave the knife in this state for 15-20 minutes. Relax,
go have a cup of coffee, whatever. Then try lowering the fluid to the proper
level. This worked well for me.

2. For really difficult knives that refuse to wet, just a very little bit of
saliva on the end of a dalmatian hair works great. A light brush against the
tongue, then drag the hair in the boat fluid behind the knife edge. I know
it sounds gross, but it works! Others have used Alconox crystals,
detergents, etc. to break the surface tension, but good old saliva works
every time.

Hope this helps.

Cheers,

Bob Chiovetti
GTI Microsystems



From daemon Fri Apr 28 08:06:21 2000



From: Mohamed Belhaj :      mohamed.belhaj-at-univ-reims.fr
Date: Fri, 28 Apr 2000 10:28:53 +0200
Subject: Re: SEM charging effects

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IIn the following references, methods to adjust the best operating conditions
(scan rate, Energy, current ) for SEM of uncoated insulators samples are
proposed and discussed.

D. C. Joy, Scanning 11, 1 (1989).
D. C Joy and C. S. Joy , Micron. 27, 247 (1996).

Good luck



*****************************************************************
Mohamed Belhaj
UFR SCIENCES
Laboratoire d'Analyse des Solides Surfaces et Interfaces
DTI/LASSI UMR CNRS
BP 1039
Reims 51687 Cedex 2
Tel : (00 33) 03 26 91 33 27: (00 33) 03 26 91 33 14
Fax : (00 33) 03 26 91 33 12
******************************************************************



From daemon Fri Apr 28 08:06:26 2000



From: DDKJoe-at-aol.com
Date: Fri, 28 Apr 2000 07:24:07 EDT
Subject: Re: diamond knife problems

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Karen,

Bob Chiovetti's ideas are right on the mark. It sounds crazy but saliva
works wonders. The only thing I would change would be the tool to use to
apply the saliva. It would probably be safer if you used the styrofoam stick
provided by many diamond knife or EM supply folks. Clean the knife with the
stick according to the instructions using a "bit of spit". Follow this up
with DI water since saliva is surprisingly dirty.

Should restore any wetting properties your old knife has left.

Have fun,
Joe Tabeling
Delaware Diamond Knives, Inc.
800-222-5143



From daemon Fri Apr 28 08:30:24 2000



From: Emrah Yucelen :      hvtem-at-yahoo.com
Date: Fri, 28 Apr 2000 08:07:46 -0500
Subject: information about TEM and radiation

Contents Retrieved from Microscopy Listserver Archives
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dear colleagues,


I wonder about TEM and radiation emitting.
is there some dangerous situation for old TEMs?
does anybody have an information about this.

Emrah YUCELEN


IstanbulTechnical University
Metallurgical
and Materials Engineering

ElectronMicroscopy Laboratory


__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Fri Apr 28 15:43:36 2000



From: Jan Watkins :      jwatkins-at-conv1.nrlssc.navy.mil
Date: Fri, 28 Apr 2000 08:53:18 -0500
Subject: Please post this question

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I am interested in getting information regarding processing images,
specifically clay fabric using Adobe PhotoShop and Image Tool, (plug-ins and
"The Image Processing Handbook"). Can anyone provide me with information on
a short course or 3 to 5 day workshop on this subject?



From daemon Fri Apr 28 15:43:38 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 28 Apr 2000 08:52:11 -0700
Subject: Re: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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} I am turning to all of you as a last ditch effort to see if my diamond
} knife is crazy or I am. I have been using this same knife for years
} (approximately 4 years) with the occasional difficulty. However, in the
} past few weeks I have been having a very hard time getting sections. The
} problem is this: water in the boat either wets the block or pulls away from
} the knife edge. There seems to be no in-between. Every once in a while, the
} stars seem to align, and the level achieves perfection for a few sections.
} I've tried several different cleaning methods, thinking that maybe the
} knife was dirty since I've been sectioning a lot of LR White. The only
} thing I've noticed (since I've been looking so very closely at the diamond)
} is that the seal (which was damaged when the knife arrived from the
} manufacturer) between the knife and the boat has detached. I would think
} that this would cause a leakage problem, which I haven't seen, not the
} problem I'm experiencing.

} Kristen -

Sounds like you're describing a slow leak, which will lower the water level
& wet the back of the knife. Try the "old-fashoned" knife sealing method:
Melt a bit of dental wax on your slide-warmer, and warm the knife gently
while the wax is melting. Use a toothpick to run a bit of wax into the
cracked seal.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Fri Apr 28 15:43:39 2000



From: Jo Dee :      jofish-at-burnham-inst.org
Date: Fri, 28 Apr 2000 09:39:25 -0700
Subject: Re: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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This sounds crazy, but my new knife came from Micro Star Diamond knives, and they
recommend only using dish detergent to wash their diamond knives. When I tried
this, the first thing that I noticed is how easy it is to keep the knife wet. I
first tried it with a problem knife, and it worked! Also, I don't have the block
face wetting problem that I had had before.
Try it, you'll be surprised.
Jo Dee
PS. I am not affiliated with Micro Star!

"RCHIOVETTI-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} In a message dated 04/27/2000 5:27:39 PM US Mountain Standard Time,
} kalen-at-citrus.ucr.edu writes:
}
} { { water in the boat either wets the block or pulls away from
} the knife edge. There seems to be no in-between. Every once in a while, the
} stars seem to align, and the level achieves perfection for a few sections.
} I've tried several different cleaning methods, thinking that maybe the
} knife was dirty since I've been sectioning a lot of LR White. The only
} thing I've noticed (since I've been looking so very closely at the diamond)
} is that the seal (which was damaged when the knife arrived from the
} manufacturer) between the knife and the boat has detached. I would think
} that this would cause a leakage problem, which I haven't seen, not the
} problem I'm experiencing. } }
}
} Kristen,
}
} I had a knife that behaved similarly once upon a time. Let's assume the boat
} isn't leaking, since you aren't having problems with water collecting
} underneath the knife in the knife holder, correct? It would be difficult to
} imagine the knife has come loose in the boat, but that may be a very slight
} possibility. If it were loose in its mount it might not section with
} consistency. Either way, it would be a good idea to have the knife re-sealed
} properly.
}
} The knife I had was almost impossible to get the proper amount of wetting on
} the diamond, but two things helped:
}
} 1. Try deliberately overfilling the boat to give a positive meniscus right
} at the knife edge. Leave the knife in this state for 15-20 minutes. Relax,
} go have a cup of coffee, whatever. Then try lowering the fluid to the proper
} level. This worked well for me.
}
} 2. For really difficult knives that refuse to wet, just a very little bit of
} saliva on the end of a dalmatian hair works great. A light brush against the
} tongue, then drag the hair in the boat fluid behind the knife edge. I know
} it sounds gross, but it works! Others have used Alconox crystals,
} detergents, etc. to break the surface tension, but good old saliva works
} every time.
}
} Hope this helps.
}
} Cheers,
}
} Bob Chiovetti
} GTI Microsystems



From daemon Fri Apr 28 15:43:39 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 28 Apr 2000 11:03:32 -0500
Subject: thanks for digital camera info

Contents Retrieved from Microscopy Listserver Archives
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I just want to say thanks for all the replies about the digital camera for
copy stand use. The info is much appreciated.
best regards,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************




From daemon Fri Apr 28 15:43:39 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Fri, 28 Apr 2000 08:34:17 -0700
Subject: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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Kristen,
It may be that after 4 years your knife edge needs a good cleaning.
I've found that when I start to have trouble wetting the knife edge I can
treat/clean the knife with a dilute solution of household ammonia.
However, remember the knife housing is made of anodized aluminum,
prolonged exposure to ammonia will degrade the aluminum and the diamond
bonding. After soaking your knife and cleaning it in your normal way,
leave it wet, and clean it again in a dilute solution of ammonia, carefully
cleaning the knife edge with a pithwood stick. Then rinse thoroughly in
flowing water for several minutes.

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Kristen Lennon [SMTP:kalen-at-citrus.ucr.edu]
Sent: Thursday, April 27, 2000 3:34 PM
To: Microscopy-at-sparc5.microscopy.com


Hi All,
I am turning to all of you as a last ditch effort to see if my diamond
knife is crazy or I am. I have been using this same knife for years
(approximately 4 years) with the occasional difficulty. However, in the
past few weeks I have been having a very hard time getting sections. The
problem is this: water in the boat either wets the block or pulls away from
the knife edge. There seems to be no in-between. Every once in a while, the
stars seem to align, and the level achieves perfection for a few sections.
I've tried several different cleaning methods, thinking that maybe the
knife was dirty since I've been sectioning a lot of LR White. The only
thing I've noticed (since I've been looking so very closely at the diamond)
is that the seal (which was damaged when the knife arrived from the
manufacturer) between the knife and the boat has detached. I would think
that this would cause a leakage problem, which I haven't seen, not the
problem I'm experiencing.
Does anyone have any advice?
Thanks for your help,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu




From daemon Fri Apr 28 15:43:40 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Fri, 28 Apr 2000 12:11:41 -0700
Subject: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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I want to thank everyone who responded to my plea for help with my diamond
knife problems. I am trying a variety of your suggestions. The strange, but
overwhelming theme involved saliva as a wetting and even a cleaning agent.
As I sit here (after trying a couple of suggestions), a beautiful ribbon of
sections is forming in the boat. I hope that it holds up.
Thanks,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Fri Apr 28 15:43:41 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 28 Apr 2000 10:06:15 -0400
Subject: Re: information about TEM and radiation

Contents Retrieved from Microscopy Listserver Archives
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Emrah Yucelen wrote:

} I wonder about TEM and radiation emitting.
} is there some dangerous situation for old TEMs?
} does anybody have an information about this.
}

Dear Emrah,
There could be x-rays generated by some old
TEMs. We had a JEOL JEM-200 which emitted a
stream of x-rays near the specimen airlock. Not only
did this make it a bad idea to stand up at the micro-
scope when the beam was on, it was also a danger for
other people in the adjacent lab. The best way to see
if there is a problem is to use a monitor (either a
Geiger counter or an ionization chamber) and survey
all around the instrument with the beam on, a heav-
ily-stained specimen in the scope, and the settings
at extreme positions. You want to survey at the
worst possible conditions that a user could set up.
Remember that x-rays can penetrtate the floor, so
the area beneath the scope should be surveyed also
(unless the scope is in the basement with no one
able to work beneath it). It should take only a few
hours to complete such a survey. Shielding for ~100
kV scopes is not too difficult in case there are radia-
tion leaks. Resurvey after the shielding is in place.
Good luck.
Yours,
Bill Tivol



From daemon Fri Apr 28 15:43:41 2000



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.Princeton.EDU
Date: Fri, 28 Apr 2000 15:47:39 -0400
Subject: Bad Probes & Misinformation

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K. Spencer wrote about Molecular Probes' Bodipy phalloidin not working or
being excited by 488 nms, 568 and 647 laser lines. I would like to know
which Bodipy you are referring to. I find it improper and misleading to
talk about a reagent unless you are going to include your specifics. I have
used several of these reagents in the past with excellent results. If you
checked the probes catalogue you will note that there are 3 Bodipy
phalloidins listed ( Bodipy 529/547, 558/569 and 584/592 ) along with
several Bodipy phallicidins that also bind to filamentous actin. These are
Bodipy 502 /512 and Bodipy-TR-X 589/617. The numbers refer to the
excitation peak and the emission peak respectively. None of these probes
should be excited with any efficiency by a 647 laser. You might see some if
you are over stained with the 584/592, a common mistake with my users.
This probe would be excited by a 568 laser but not by a 488 nm line.
Secondly, before buying a probe, one is advised to find out what is
excitation and emission spectra are ( not just peak numbers) so you know
what possible cross talks one could possibly experience.

For these reagents I recommend the Bodipy 505/512 or Oregon Green for 488
excitation. AlexaFluor-568 phalloidin or rhodamine phalloidin for
excitation with 543 or 568 laser. The Alexafluors would be my top choice
because of superior spectral properties. I know of none that work with a
633 or 647 laser. Cy 5 is my preferred choice for the far red channel as a
conjugated antibody and TOTO-3 as a DNA marker.

Recently someone complained of TOTO-3 bleaching as they imaged. The dye
doesn't bleach, but rather gets displaced from the DNA when laser excites
it. It only fluoresces when bound to DNA or RNA. The trick here is to use
the lowest laser or excitation you can, and to include the dye at about 1-2
micromolar in your mounting medium so it can re-intercalate into the DNA.


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
{http://www.molbio.princeton.edu/facility/confocal/index.html}

jgoodhouse-at-molbio.princeton.edu {mailto:Jgoodhouse-at-molbio.princeton.edu}
609-258-5432



From daemon Fri Apr 28 18:07:53 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 28 Apr 2000 13:51:09 -0700 (Pacific Daylight Time)
Subject: Re: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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Hello Kristen,

I concur with Bob Chiovetti's suggestions as possible
solutions to aid in wetting of the diamond knife edge and
eliminate block wetting. In particular, it seems that
adding a bit of saliva to your eyelash tool, which is then
wiped across the flat portion of the diamond, is very
effective. In extreme cases we have subjected the knife to
ionized air via glow discharge but this can cause water to
be drawn to the back side of the diamond. Naturally, you
should check the diamond for cleanliness. It may help to
immerse the knife in soapy water after careful cleaning
with ethanol dipped Styrofoam. After rinsing the soap away
with purified water, we then rinse the boat in ethanol
which also seems to help wetting of the edge.

Good luck,

Doug
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Fri Apr 28 18:07:54 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 28 Apr 2000 11:24:02 -1000 (HST)
Subject: Re: Holder Filter

Contents Retrieved from Microscopy Listserver Archives
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}
} I am searching some device for holding (polycarbonate)filters during
} dehydration and CPD. I am trying to view mycoplasms with SEM. Has anyone
} experience with this ?
}
We have a device from Tousimis that is designed to hold round glass
coverslips, and I have used it to hold polycarbonate filters. It is a
milled-out cylinder open along one side. Wavy washers are used to
separate the stacked coverslips. There are two sizes, 13 mm and 22 mm,
part numbers 8766 and 8767. You can contact Tousimis at 1-800-638-9558,
fax 1-301-881-5374, http://www.tousimis.com

This device works for me! My only affiliation with Tousimis is as a
satisfied customer.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Fri Apr 28 18:07:55 2000



From: anderron-at-us.ibm.com
Date: Fri, 28 Apr 2000 10:58:41 -0400
Subject: Lehigh TEM Specimen Preparation Course

Contents Retrieved from Microscopy Listserver Archives
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The TEM specimen preparation course advertised in the Lehigh "2000
Microscopy School" booklet, p. 20., has undergone revision. This is a
completely new course that was not finalized when it was time to print the
booklet. The material on p.20 was a place holder.

Scott Walck joined with me in organizing the course and we have secured
commitments from a large number of specimen preparation tool vendors to
provide staff and equipment for the course, listed below.

Title:

TEM Specimen Preparation 2000
With Emphasis on Recently Developed Tools and Methods

Course Description:

This course is designed to provide classroom instruction and laboratory
demonstration of the newest methods for preparing SEM and TEM specimens.
While an individual with no experience preparing specimens will benefit
greatly from the course, the intended audience will consist of students
with some degree of proficiency utilizing classical methods of specimen
preparation and who wish to update their capabilities. Emphasis will be
placed on the rapid preparation of specimens from very small pre-selected
locations. The preparation of SEM samples will be treated as the first
step in making TEM specimens. "Hands on" experience by the students will
be available. Table-top exhibits and demonstrations of specimen
preparation ancillary equipment, such as: saws, dimplers, disc cutting
tools, etc., will be available during the lab periods. TEM examination of
prepared specimens will be performed.

Instructors:

Ron Anderson, IBM Analytical Services and Scott Walck, PPG Industries, Inc.

Outline:

Thursday, June 22

10:00 am Registration (Whitaker Lobby)
1:00 pm Introduction Anderson/Walck
1:15 pm Specimen Preparation Flowchart,
Initial Considerations, Recent Advances,
Choice of Technique Anderson
2:15 pm Initial Thinning, Tools, Disk Cutting,
Dimpling Walck
3:30 pm Mechanical Polishing Anderson
4:30 pm Automated Initial Preparation Methods Vendors
Sawing: Sagitta
Cleaving: SELA
7:45 pm Ion Milling Anderson
Commercial Tool Overview Walck

Friday, June 23

8:30 am Lab: Tripod Polishing
Lab: Sagitta Tool
Lab: SELA Tool
11:00 am Focused Ion Beam (FIB) Methods Anderson/Walck
2:00 pm Lab: FIB Methods
Lab: Ion Mill Tools

Saturday, June 24


8:30 am Mechanical Polishing Difficult Materials Anderson
9:00 am Ultramicrotomy Walck/Anderson
10:00 am Small Angle Cleavage Technique Video Igor
10:30 am Plasma Cleaning Specimens Walck
11:00 am Reactive Sample/Storage/Transporting Walck
11:15 am Lab: Student Use of Available Tools
Lab: TEM Examination of Prepared
Specimens. Discussion of Successes
and Problems

Vendors providing instrumentation and staff:
v Allied High Tech
v Bal-Tec
v FEI
v EA Fischione
v Gatan
v Sagitta
v SELA
v South Bay Technology

The registration deadline is June 1st. Registration forms are in the
"Lehigh Microscopy School" booklet, or they may be obtained, along with
accommodation information etc., from :

Ms. Sharon Coe
Dept. of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem, PA 18015-3195

(610)758-5133
(610)758-4244 FAX
SHARON.COE-at-LEHIGH.EDU

www.lehigh.edu/~inmatsci/Microscourses.html






Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg





From daemon Fri Apr 28 18:07:56 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 28 Apr 2000 12:23:40 -0600 (MDT)
Subject: Re: diamond knife problems

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On Fri, 28 Apr 2000 DDKJoe-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Karen,
}
} Bob Chiovetti's ideas are right on the mark. It sounds crazy but saliva
} works wonders. The only thing I would change would be the tool to use to
} apply the saliva. It would probably be safer if you used the styrofoam stick
} provided by many diamond knife or EM supply folks. Clean the knife with the
} stick according to the instructions using a "bit of spit". Follow this up
} with DI water since saliva is surprisingly dirty.
}
} Should restore any wetting properties your old knife has left.
}
} Have fun,
} Joe Tabeling
} Delaware Diamond Knives, Inc.
} 800-222-5143
}
}
Hi,

Saliva is dirty and full of debris and bacteria. Use a wetting agent
instead as above and fill the boat with it or draw it across the edge
whichever works best.

Hildy Crowley



From daemon Fri Apr 28 18:07:56 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 28 Apr 2000 12:20:42 -0600 (MDT)
Subject: Re: diamond knife problems

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On Thu, 27 Apr 2000, Kristen Lennon wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Hi All,
} I am turning to all of you as a last ditch effort to see if my diamond
} knife is crazy or I am. I have been using this same knife for years
} (approximately 4 years) with the occasional difficulty. However, in the
} past few weeks I have been having a very hard time getting sections. The
} problem is this: water in the boat either wets the block or pulls away from
} the knife edge. There seems to be no in-between. Every once in a while, the
} stars seem to align, and the level achieves perfection for a few sections.
} I've tried several different cleaning methods, thinking that maybe the
} knife was dirty since I've been sectioning a lot of LR White. The only
} thing I've noticed (since I've been looking so very closely at the diamond)
} is that the seal (which was damaged when the knife arrived from the
} manufacturer) between the knife and the boat has detached. I would think
} that this would cause a leakage problem, which I haven't seen, not the
} problem I'm experiencing.
} Does anyone have any advice?
} Thanks for your help,
} Kristen
} Kristen A. Lennon
} Cell, Molecular & Developmental Biology Group
} Department of Botany & Plant Sciences
} University of California
} Riverside, CA 92521
} kalen-at-citrus.ucr.edu
}
}
The detachment of the knife from its mount may be the cause. It has
happened in our laboratory. Try, however, to add one drop of Photo-flo to
about 50ml of filtered or distilled water. Try that in the boat.
Detachment tends to be a serious problem! Send it back and have it
repaired and resharpened. After 4 years it probably needs it.

Hildy Crowley



From daemon Fri Apr 28 18:07:56 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Fri, 28 Apr 2000 15:09:12 -0700
Subject: parts from old equipment

Contents Retrieved from Microscopy Listserver Archives
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Spring cleaning and new equipment requires I dispose of a Mikros VE10
vacuum evaporator and a LKB 4800 ultramicrotome. Anyone needing parts
from these old, non-functional units please contact me.

steve


Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/emfacility/

Chairman, Educational Outreach subcommittee
promoting access to microscopes
Microscopy Society of America http://www.msa.microscopy.com/




From daemon Sat Apr 29 08:15:09 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 28 Apr 2000 22:46:33 -0500
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
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Beth,

You may want to consider a digital camera that is a SLR camera. I have a
Kodak DC 120 and it works reasonably well but when I want to take close up
photos or copy stand shots, I have to take several exposures to get the
subject critically centered. Of course, a SLR allows me to set up my photo
exactly the way I want it; also getting the correct lighting is
easier. For this kind of work I use a Kodak DCS 420, but at ~$12k it's a
"bit expensive".



} Hi all,
} This is off the microscopy subject...sorry.
} Any recommendations on digital cameras for copy stand work and general
} photography? Has anyone tried the Nikon Coolpix 990?
} We're coming up on the end of the fiscal year and there might be funds
} available for a digital camera so any advice is greatly appreciated.
} thanks,
} Beth Richardson

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Sat Apr 29 08:15:09 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 28 Apr 2000 19:47:33 -0500
Subject: Re: Kodak MDS 120

Contents Retrieved from Microscopy Listserver Archives
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The Kodak MDS 120 does a very good job for the price. My only
recommendation is that you get the flash memory cards (I have a 64 MB card)
and a card reader or adapter for a PCMCIA slot. Transferring the images
via the built-in serial port is, of course, INCREDIBLY SLOW!!! The
included software works well and you can easily get the images into other
software such as Adobe Photoshop.




} Part of the package which we got is a 16 MB Flash Memory card for the camera,
} so you can either run the camera from your computer or store images on the
} memory card and then remove the card and take it to a computer to transfer
} the images to the computer. The package also had a PCMCIA card adapter in
} it. The memory card plugs into the end of the PCMCIA device, and you can
} then slip it into a PCMCIA slot on a laptop computer.
}
} But if you need to use a desktop computer and don't have a PCMCIA slot, you
} need to get something like a SanDisk card reader for the computer. They're
} available at CompUSA, Fry's Electronics, OfficeMax, etc. for about $70.
}
} The software with the camera is very nice. It lets you transfer images from
} the camera's memory directly or from from the memory card, preview images and
} download them directly from the camera, etc. as well as perform some basic
} image adjustments (flip, rotate, modify brightness, contrast, gamma, etc.)

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Sat Apr 29 08:15:10 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 28 Apr 2000 22:45:07 -0500
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just returned from a course on VP SEM (which was excellent) and just now
can add to some of the digital camera discussion.

One thing not mentioned about using a SLR digital camera like the E1, N60
is vibration from the mirror and the shutter. If you are going to use a
camera of this type you must be able to lift the mirror independently, like
the old F1, whether it's film or CCD. I have a Kodak DCS420 that is based
on the Nikon N90s camera body and it works ok at low magnifications but
anything over about 200X just doesn't cut it.



At 08:33 AM 4/26/00 -0700, Dr. Gary Gaugler wrote:
} The Fuji Super CCD is 23.3 x 15.6mm; 3040 x 2016 pixels.
} The S1 Pro uses Nikon mount lenses and has an equivalent
} 35mm frame size at 1.5X the lens' focal length.
}
} What the equivalent FOV would be on a scope is still in
} question. If the S1 Pro works anywhere near how the
} Nikon E1 or E2 does, I fear the Fuji camera won't be applicable
} to microscopy at all. Without a lens, the camera must operate
} in aperture priority mode and center weighted exposure
} reading. Unless Fuji made some major changes to the basic
} Nikon (N-60) body and associated electronics, they may have
} just made a higher pixel count Nikon digicam.

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Sat Apr 29 09:45:31 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 29 Apr 2000 07:34:07 -0700
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
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Neither the N60 or N80 bodies have mirror lock up. However,
at equivalent ISO of 400-1600, it remains to be seen if lock
up is necessary. And also, how ISO affects the resulting
image. Based on how the E1 and E2 worked, I would not
count on a miracle.

The Kodak 420 is obsolete and discontinued. These can be
purchased used today for somewhere in the $2K-$4K range.
But, with its tiny CCD, I would not have one again. The newer
models like DCS460 have good features. But I have not
been impressed by Kodak's tech support user friendliness.
It would be a hard sell for me to buy any Kodak product these
days over other manufacturer's offerings.

The mechanical shutter is a definite problem, both for image
stability as well as camera reliability. The Leaf Lumina has a
shutter but it operates only one time for each shot. It is very
reliable. In contrast, the Polaroid DMC has a shutter that seems
to always be "shuddering" the system. I would expect that the
DMC would exhibit a poor reliability record. The advantage of
the DMC is that it provides near-real time focusing through the
camera's imager. The only other camera I have used that
exceeds this is the Sony DKC-5000 (cat's eye) digital camera.
But the DKC-5000 uses a very small CCD as well and suffers
from tiny images. But focusing is true real time via a separate
RGB color monitor. When a frame is shot, the main system
box transfers the image to the computer via SCSI.

gg

At 08:45 PM 4/28/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Apr 30 09:37:43 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 29 Apr 2000 17:56:06 -0700
Subject: Freon 113 dehydration

Contents Retrieved from Microscopy Listserver Archives
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When using F-113, I preceed it with either or both
methanol and acetone. The curious problem is that
after either a lengthy immersion in acetone or methanol,
the specimen (insect) floats when put in F-113. OK.
So it is lighter than F-113. What is a good method
of encompassing the specimen with F-113 to further
dehydrate it prior to vacuum desiccation?

Any ideas?



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Apr 30 09:37:46 2000



From: John Foust :      jfoust-at-threedee.com
Date: Sat, 29 Apr 2000 21:18:51 -0500
Subject: Introductory message

Contents Retrieved from Microscopy Listserver Archives
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I'm new to the list. I recently picked up a delightful
Leitz Ortholux I, like at http://arsmachina.com/leitz_ortholux.htm
and a non-working Zeiss OpMi-1 surgical scope, similar to
the upper left quadrant of http://www.mednetlocator.com/opmi-1.gif
with not much arm and no stand. I think it's missing a lense
from the underside.

I'm temporarily illuminating the Ortholux with the 5V DC from a
spare PC power supply. The lamp says 6 volts, I think. What's
the voltage range from the original rheostat-style power supply?

- John



From daemon Sun Apr 30 09:37:49 2000



From: COURYHOUSE-at-aol.com
Date: Sun, 30 Apr 2000 00:03:43 EDT
Subject: Re: Introductory message

Contents Retrieved from Microscopy Listserver Archives
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Hi John!
5 volts is fine, the color temperature is a bit warmer but your bulb will
last longer!
I usually only run the bulb at full or over when I am taking a photograph.
For normal observation I find the 5 volts fine. Congrats on the othrolux
great classic scope!
Ed Sharpe archivist for SMECC

{ { Subj: Introductory message
Date: 4/29/00 8:53:44 PM US Mountain Standard Time
From: jfoust-at-threedee.com (John Foust)
To: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.



I'm new to the list. I recently picked up a delightful
Leitz Ortholux I, like at http://arsmachina.com/leitz_ortholux.htm
and a non-working Zeiss OpMi-1 surgical scope, similar to
the upper left quadrant of http://www.mednetlocator.com/opmi-1.gif
with not much arm and no stand. I think it's missing a lense
from the underside.

I'm temporarily illuminating the Ortholux with the 5V DC from a
spare PC power supply. The lamp says 6 volts, I think. What's
the voltage range from the original rheostat-style power supply?

- John


} }



From daemon Sun Apr 30 09:54:03 2000



From: Vera Shinder :      emshin-at-wis.weizmann.ac.il
Date: Sun, 30 Apr 2000 17:42:37 -1200
Subject: Ion Milling

Contents Retrieved from Microscopy Listserver Archives
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Hi , all!

Does anybody knows something about Ion Milling applications in
biology? Thanks, Vera.



From daemon Sun Apr 30 17:39:28 2000



From: Edsworth-at-aol.com
Date: Sun, 30 Apr 2000 16:49:23 EDT
Subject: Re: kodak digital camera

Contents Retrieved from Microscopy Listserver Archives
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Ron: I've been using the Kodak DS120, which is the camera that is used in the
system you asked about, and have had good results. There are a few things to
keep in mind, however. The DS120 saves images to flash cards using a
propriatary Kodak method that is not recognized by anything else. Therefore,
some of the nifty print systems can't directly use the images. I use the
Kodak-supplied software to copy the images from the flash card to hard disc
via a card reader (SanDisk -- cheap and works real well). Once in my
computer, I work on the images using PhotoShop. Final printing is performed
on my Epson Photo 1200 using print paper. The results are very good.

I do note that when using the DS120 with the MDS adaptor on my Nikon
microscope that there is a bright area in the center. I'm not sure who's
fault that is.

The small viewing screen is a problem, but once the focus is properly set it
is not a major disadvantage. With the system you are talking about
(remember, I just have the camera and adaptor) it may be that the image can
be displayed prior to collecting it onto the flash card.

Overall, I think it's a good value for the money.

If you'd like to talk about the camera, connect off line at edsworth-at-aol.com.

Hope this has helped a little.



From daemon Mon May 01 07:59:58 2000



From: oshel-at-terracom.net (Philip Oshel)
Date: Sun, 30 Apr 2000 17:58:11 -0500
Subject: Re: Freon 113 dehydration

Contents Retrieved from Microscopy Listserver Archives
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Gary,

I'm trying to remember ... Freon 113: Peldri, yes? Or is this one that
stays fluid? (And how did you get it, since Freons are mostly illegal
anymore...) .

First, you might try vacuum, 1 atmosphere or so, maybe not that much,
gently applied, and released.

For Peldri, I put specimens in small vials -- 4 mL Wheatons are my favorite
-- and completely filled the vial, then capped, leaving no air. If there
were enough alcohol:Freon intermediates, and enough changes in pure Freon,
the specimens should be in pure Freon now.

Mind, I mostly did crustaceans and fish bits, not insects with their waxy
epicuticles, but then some or all of that wax is lost in the alcohols
anyway.

But I wouldn't use methanol or acetone. Ethanol works better for insects.
Acetone is OK, but I've found ethanol better, and methanol is too
extractive.

You don't have a CPD for this? Also, some insect parts can be dried from
ethanol or even water. Mandibles of many species for instance, or the
elytra of most beetles. Not whole insects, though.

I never got my specimens to sink in Freon 13, Back When, but they usually
did sink in Peldri.

Phil

} When using F-113, I preceed it with either or both
} methanol and acetone. The curious problem is that
} after either a lengthy immersion in acetone or methanol,
} the specimen (insect) floats when put in F-113. OK.
} So it is lighter than F-113. What is a good method
} of encompassing the specimen with F-113 to further
} dehydrate it prior to vacuum desiccation?
}
} Any ideas?

}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
Voice: (608) 263-4162
peoshel-at-facstaff.wisc.edu
fax: (608) 262-7420 (dept. fax)




From daemon Mon May 01 08:00:41 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Mon, 01 May 2000 07:54:49 -0400
Subject: Re: Holder Filter

Contents Retrieved from Microscopy Listserver Archives
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Last fall we actually fabricated one of those holders from some copper water
pipe, and purchased the wavy washers from McMasters. I had seen the one Tina
mentions in use and thought "heck, we could make one and save $100." Well we
did just that. For less than $20, if you have a metal cutting saw, some solder
and access to purchasing wavy washers, you can fabricate your own.
One grad student has used it for the spring semester to dehydrate, and CPD,
poly-l-lysine coated 12mm coverslips classifying the morphology and size of
bacteria in the hind gut of Tipula abdomalis. I just reviewed his final work
and the images looked great. He had no problems with debris or samples washing
off.
The advantage of making it yourself is that you can customize the size for the
application for very little money. You would only be limited by the availibity
of sizes of wavy washers.

-Geoff

} } I am searching some device for holding (polycarbonate)filters during
} } dehydration and CPD. I am trying to view mycoplasms with SEM. Has anyone
} } experience with this ?
} }
} We have a device from Tousimis that is designed to hold round glass
} coverslips, and I have used it to hold polycarbonate filters. It is a
} milled-out cylinder open along one side. Wavy washers are used to
} separate the stacked coverslips. There are two sizes, 13 mm and 22 mm,
} part numbers 8766 and 8767. You can contact Tousimis at 1-800-638-9558,
} fax 1-301-881-5374, http://www.tousimis.com
}
} This device works for me! My only affiliation with Tousimis is as a
} satisfied customer.
}
} Aloha,
} Tina
}

--
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Mon May 01 08:00:42 2000



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 01 May 2000 07:28:11 -0500
Subject: Re: Introductory message

Contents Retrieved from Microscopy Listserver Archives
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Next question about the Ortholux: where might I find the operating
or service manuals?

I'm located in Jefferson, WI, halfway between Madison and Milwaukee.

- John



From daemon Mon May 01 08:20:50 2000



From: Esra SAVAS :      esavas-at-akasya.istanbul.edu.tr
Date: Mon, 1 May 2000 08:12:11 -0500
Subject: Scanning Electron Microscope

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Dear Sir,
I have been working for Istanbul University, Faculty of Fisheries, as a
research Assistant on aquaculture. I am a doctorate student at the
present. My studies are on Aquarium Fish ( ornamental ). The topic of my
doctoral thesis is " A Study on the Artificial Propagation of Discus
Fish ( Symphysodon spp. ) and Effective Factors on the Propagation ".
Besides, I research too my thesis for discus 's embriological-larval
developing and sperm developing with scanning elecron microscope ( SEM
). With regard to my doctoral thesis: Could you send me Scanning
Electron Microscope SEM )' s use-book and example's processing ? I wish
success your studying.
Best regards,

Esra SAVAS
I.U. Su Urunleri Fak.,
Ordu Cad., No: 200 34470
Vezneciler / Istanbul TURKEY




From daemon Mon May 01 12:33:28 2000



From: Susnitzky, David :      david.susnitzky-at-intel.com
Date: Mon, 1 May 2000 07:09:41 -0700
Subject: Announcement - N. California Local Affiliate Meeting on May 11t h

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N. California Society for Microcsopy

MAY 11TH SYMPOSIUM AT U.C. Davis
TOPIC: Electron Backscatter Patterns (EBSP)

Featured Speakers:
1) David Dingley, TSL, "Automated Crystallography for the TEM: Recent
Developments"
2) Adam Schwartz, LLNL, "Coupling Automated Electron Backscatter Diffraction
with Transmission Electron and Atomic Force Microscopies".
3) Patrick Camus, Noran Instruments, "Phase Identification versus Phase
Discrimination"
4) Pierre Rolland, Oxford Instruments, "Mapping Copper Interconnects for the
Semiconductor Industry"
5) John Sutliff, HKL Technology, "Quantifying Polycrystalline
Microstructures: The Automated-ESBP Technique and its Applications"

The symposium will be held in the AGR room in the Buehler Alumni & Visitors
Center, UC Davis

Meeting starts at 3:00
No Host Social -at- 5:30
Dinner Buffet -at- 6:30
Meeting/Dinner will end by 9:00

Dinner Buffet-
Rosemary & Orange Chicken
Fettucine Alfredo
Spring Mix Salad with Raspberry Vinaigrette
Greek Salad topped with Crumbled Feta Cheese on a Bed of Greens
Chef's Selection Vegetable
Fresh Baked Rolls with Butter
New York Cheesecake with Melba Sauce garnished with Mint
Coffee, Decaf, Hot Tea & Iced Water

Cost: $20 regular members, $10 student members, $25 nonmembers

Please call Greg Lum at 415/338-1339 or Email: "glum-at-sfsu.edu" for dinner
reservations by Monday May 8th. The symposium starts at
3:00 p.m. The dinner will start at 6:30 pm.








From daemon Mon May 01 12:33:33 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Mon, 1 May 2000 13:49:27 -0400
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
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The Nikon D1 Digital SLR camera does not have a mirror lockup but
does have an "Anti Vibration Mode" which causes the shutter opening to
be delayed until mirror shock has subsided.
The D1 has 2.7million pixels, ISO range 200-1600 and accepts
most Nikon lenses, electronic releases,etc. It is excellent for
both copystand and light microscopy applications.

George Laing
National Graphic Supply
226 North Allen Street
Albany, NY 12206
E-mail: scisales-at-ngscorp.com
(800) 223-7130 X3109
(518) 438-8411 X3109

At 08:45 PM 4/28/00 , you wrote:
}
} Just returned from a course on VP SEM (which was excellent) and just now
} can add to some of the digital camera discussion.
}
} One thing not mentioned about using a SLR digital camera like the E1, N60
} is vibration from the mirror and the shutter. If you are going to use a
} camera of this type you must be able to lift the mirror independently,
} like the old F1, whether it's film or CCD. I have a Kodak DCS420 that is
} based on the Nikon N90s camera body and it works ok at low magnifications
} but anything over about 200X just doesn't cut it.
}
}
}




From daemon Mon May 01 13:21:48 2000



From: Maria.Fazio-Zanakis-at-aventis.com
Date: Mon, 1 May 2000 13:02:50 -0500
Subject: unsubscribe

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UNSUBSCRIBE, please.

Maria Fazio-Zanakis
Bioimaging and Molecular Histology
Aventis Pharmaceuticals
1-908-231-3357
Fax: 1-908-231-3962
Email: Maria.Fazio-Zanakis-at-Aventis.com



From daemon Mon May 01 17:18:23 2000



From: Kirk Czymmek :      kirk-at-udel.edu
Date: Mon, 1 May 2000 14:56:56 -0400
Subject: Philips 201 and 501

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a Philips 501 SEM and 201 TEM that I am about to dismantle and
discard. They were both operational until four months ago. If anyone needs
them for spare parts please feel free to contact me.


Best Regards,

Kirk J. Czymmek, Ph.D.
Director, Core Microscopy Facility
Department of Biological Sciences
University of Delaware
Newark, DE 19716
kirk-at-udel.edu
PH: (302) 831-1158



From daemon Mon May 01 17:18:35 2000



From: zaluzec-at-aaem.amc.anl.gov
Date: Mon, 1 May 2000 13:35:04 -0500
Subject: PASEM 2000" - THE PRACTICAL ASPECTS SERIES OF SHORT COURSES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

If you find them let me know I would like a copy. I will pay reasonable
or slightly unreasonable charges for copying and mailing.

Gordon

G. C,. Couger
624 Cheyenne
Stillwater, OK 74075-1411
405 624 2855 between 3:00 pm to 1:00 am CST
405 742 2758 cell
----- Original Message -----
} From: "John Foust" {jfoust-at-threedee.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, May 01, 2000 7:28 AM


Meeting: "PASEM 2000" - THE PRACTICAL ASPECTS SERIES OF SHORT COURSES
Dates: May 22 through June 2, 2000
Topic: The Practical Aspects Series consists of intensive 3.5 to 4.5 day
short courses that provide participants with a thorough coverage of the
basic theory and practice of
scanning electron microscopy and associated techniques. Training is
achieved through the use
of easy to understand lectures coordinated with supervised laboratory
sessions. The
laboratory class sizes are purposely kept small so that participants will
have an opportunity to
gain extensive hands- on experience with available SEM and EDS
instrumentation. Designed for the
academic, government and industrial user, the courses are beneficial to
microscopists
and microanalysts at all levels from novice through advanced. Course titles
and dates for our
Year 2000 series follow:

1. SCANNING ELECTRON MICROSCOPY - May 22 through May 26, 2000
2. ADVANCED TOPICS IN SCANNING ELECTRON MICROSCOPY - May 30 through June
2, 2000
3. X-RAY MICROANALYSIS - May 30 through June 2, 2000

Sponsor: University of Maryland
Location: College Park, Maryland, USA
Interests: Both Physical & Biological Sciences
Fields: SEM, EDS
Contact: Tim Maugel, University of Maryland, Department of Biology,
Building 144, Room 0240, College Park, Maryland, 20742, USA
Tel: 301-405-6898
Fax: 301-314-9358
E-mail: tm11-at-umail.umd.edu
WWW: http://www.life.umd.edu/pasem




From daemon Tue May 02 07:26:40 2000



From: varguc-at-freemail.c3.hu
Date: Tue, 2 May 2000 07:17:42 -0500
Subject: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear Christian!

If some of your spectra are only available in LINK AN/10000 format,
then you could convert them to simple text files with a little
program I wrote about a year ago.
It's a 32 bit Windows program. You can select as many LINK spectrum
files (in their original, not converted format) as you need. All the
selected files will be converted in one run. I have tried
it with more than 1700 spectra. The result of the conversion is a
text file with the same name but with different file extension. An
example from a converted spectrum file (from a series):
-------------------------------LAK11S103.SP---------------------------
ak11s** 3

preset live time: 40
live time: 40
real time: 49
20.000000 eV/channel

Energy[keV], counts
-0.200000, 0
-0.180000, 0
-0.160000, 0
-0.140000, 0
-0.120000, 3
-0.100000, 16
-0.080000, 42
-0.060000, 98
..
----------------------------------------------------------------------
If you are still interested in a program like this, drop me a line
and I'll send it to you.

With the best regards:
Laszlo Varga


On 20 Apr 00, at 10:21, Filion, Christian wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good morning,
}
} I have both a Spectra file in Link format and txt format saved as a MSA
} type file. Is there a software or an excel macro that could read these
} spectra ?
}
} Thank you
}
} Christian Filion
} Superviseur Laboratoires
} Aciers Inoxydables Atlas
} 1640 Marie-Victorin
} Tracy, Québec
} J3R 5R5
} Tel : 450-746-5243
} fax : 450-746-5241
}
}




From daemon Tue May 02 07:26:45 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 2 May 2000 07:14:20 -0500
Subject: Microscopy & Microanalysis 2000 Search Engine Now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

For those of you interested the Microscopy and Microanalysis 2000 Meeting
Search Engine is now on line.

Using the Search engine you can find out dates/times/locations
of any author, paper, subject or session at the upcoming meeting this
August in Philadelphia.


Just go to http://www.msa.microscopy.com

and follow the links to the M&M 2000 meeting.

See you in Philly....

Cheers....

Nestor
Your Friendly Neighborhood SysOp




From daemon Tue May 02 15:57:33 2000



From: nessler :      randy-nessler-at-uiowa.edu
Date: Tue, 02 May 2000 08:36:33 -0500
Subject: SEM Peltier stage vendors?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for vendors of Peltier cooling stages for our Hitachi
2460N VPSEM. If you know of a company, please drop me a line. Also, if
you have opinions on what features you would look for in such an item,
I'd like to hear it.
Thanks in advance,
Randy
--
Randy Nessler
Views expressed are my own.



From daemon Tue May 02 15:57:33 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 2 May 2000 09:37:46 -0400 (EDT)
Subject: Re: Drive Belt for Pyramitome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Never replaced a Pyramitome belt - but LKB microtome belts can be replaced
by "regular" belt material - just match the width and springiness (did you
keep any of the old belt fragments?). If your institution doesn't have a
machine shop (those guys usually have extra belts floating around), try
your local hardware store/superstore. I've had good luck replacing
standard parts this way, and it is probably going to be light years
cheaper than an "official" replacement part, if such a thing is available.

} From experience, giant rubber bands do not work very well.........

Tamara Howard
CSHL

On Tue, 2 May 2000 mganger-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Date: Tue, 2 May 2000 08:08:07 EDT
} Subject: Drive Belt for Pyramitome
} To: Microscopy-at-sparc5.microscopy.com
} MIME-Version: 1.0
} Content-Type: text/plain; charset="US-ASCII"
} Content-Transfer-Encoding: 7bit
} X-Mailer: AOL 5.0 for Windows sub 104
}
} Greetings,
}
} I would like to know if anyone out there knows of a repair place that sells
} parts for an LKB 11800 Pyramitome. The drive belt for the specimen arm has
} literally fallen apart and I need to replace it. Any suggestions on where I
} could get one would be greatly appreciated.
}
} Thanks in advance.
}
} Mike Ganger
} Montclair State University
} Montclair, New Jersey
} mganger-at-aol.com
}
}
}
}



From daemon Tue May 02 15:57:37 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 02 May 2000 10:28:58 -0700
Subject: Re: Drive Belt for Pyramitome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I would like to know if anyone out there knows of a repair place that sells
} parts for an LKB 11800 Pyramitome. The drive belt for the specimen arm has
} literally fallen apart and I need to replace it. Any suggestions on where I
} could get one would be greatly appreciated.

Be sure to look at vacuum cleaner drive belts. They come in many sizes and
I've used them as drive belts for a lot of devices. They look like a giant
O-ring, up to 1/4 inch thick and in many lengths.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Tue May 02 16:04:29 2000



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Tue, 02 May 2000 14:00:35 -0700
Subject: Two places left

Contents Retrieved from Microscopy Listserver Archives
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Ernest Orlando Berkeley National Laboratory
One Cyclotron Road, Berkeley, California 94720
FOR IMMEDIATE RELEASE

Two places left for students to attend the
NCEM Summer School on Computing in Electron Microscopy
June 26-30, 2000 in Berkeley, California


(Berkeley, CA) The seventh annual Summer School on Computer-Interactive
HRTEM Image Acquisition, Processing and Simulation will be held at the
National Center for Electron Microscopy (NCEM), Lawrence Berkeley
National Laboratory, University of California, Berkeley from June 26
through June 30, 1999.

The curriculum will focus on training participants in techniques of
computer-assisted acquisition and interpretation of high-resolution
electron microscope images, including HRTEM image processing and
simulation, electron holography, focal-series reconstruction, and
remote-control microscopy. Participants will learn general principles
and apply them to specific cases. Instruction will focus on the use of
computer assistance rather than microscope training, although
participants will acquire images on NCEM microscopes as well as using
specific application programs for image interpretation. Class size
will be limited to 16. Deadline for applications, May 01, 2000, has
been extended to May 04, 2000..

Participants who wish to apply newly acquired techniques to their own
projects will be encouraged to extend their visit at NCEM into the next
week. Please note: this type of arrangement requires advance submission
of an NCEM microscope proposal (see:
http://ncem.lbl.gov/frames/user.htm). Projects may involve prepared
specimens for microscopy, images and diffraction patterns for
processing, or crystal and defect data for simulations.

For more information and application materials, contact:

Website: http://ncem.lbl.gov/frames/workshops.htm#workshops
email: JLCavlina-at-lbl.gov
Phone: 510/486-6036
Fax: 510/486-5888.


# # # #




From daemon Tue May 02 20:43:12 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 02 May 2000 17:57:56 -0400
Subject: LM: Stains for polymers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have been reading a great deal on polymer microscopy lately and came
across the statement that there was very little information to be gained by
staining polymers and (as might be expected) that they did not take up
stain well. Many years ago, I had heard of an application in which osmium
tetroxide was used, I believe on polyethylene. Can anyone shed any light,
either on the general comment or on the application using osmium tetroxide?

Many thanks,
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



From daemon Tue May 02 20:43:19 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 2 May 2000 18:58:46 -0500
Subject: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a good, safe way of mounting Codonics dye
sublimation prints onto poster boards? We would like to avoid the
spray on "rubber cement" types of glues due to the mess.

Thank you.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Wed May 03 08:26:49 2000



From: Richard E. Edelmann :      edelmare-at-casmail.muohio.edu
Date: Wed, 3 May 2000 06:39:20 -0500
Subject: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Double sided tape or a product that picture framers use that
just the glue without the tape. It is on a tape that releases
the glue. It does an excellent job of mounting. I have pictures
that were mounted with the Scotch brand 20 years ago that
are just as good as the day I did them. It will also stick to
the plastic use in resin coated photographic paper.

Any picture framing shop should have it.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "John J. Bozzola" {bozzola-at-siu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 02, 2000 6:58 PM


O.k., whereas not directly a microscopy question I was hoping some one would
have a solution. Look for something that will function like aluminium foil but be non-
reflective.

(1) shapable around objects (we're covering petri dishes), but not too massive

(2) be opaque (visble light 420-720nm)

(3) be non-reflective on at least one side. "Non-reflective": less than 15%
reflectivity to visible spectrum 420-720nm.


Any suggestions? (Any good ideas for turning Al foil nice and black?)

Thanks.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Wed May 03 08:26:49 2000



From: Gary Coulton :      g.coulton-at-ic.ac.uk
Date: Wed, 03 May 2000 12:04:14 +0100
Subject: ICHC 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

There is still time to register for:

11th International Congress of histochemistry and Cytochemistry (ICHC 2000)

Cell Biology Tools for the New Century

ICHC 2000 is the premier meeting this year to address the very latest
developments in visualisation techniques for Cell Biology. to be held in
the magical medieval city of York, England between 3-8 September 2000.

We have a fabulous speaker listof over 100 including some of the biggest
names in Cell Biology and Imaging such as Roger Tsien, Richard Haugland,
Fred Bosman, Alan Boyde, Stefan Hell, Alan Fine, Jim Smith, Margaret
Buckingham, Nick White, Angus Lamond, David Becker, Jeniffer
Lipincott-Schwartz and many, many more.

Topics include -Tracking Molecules in Cells, Fluorescent markers of Gene
Expression, Live Cell Imaging in plants and Fungi, Apoptosis, Cell
Signalling, Environmental toxicology, Imaging Embryonic Development, Ion
Imaging in Cells, Imaging in the Neurosciences etc., etc. etc.

See the science and have a chance to talk to the best in the field.

Come for a holiday as well as the science we can offer many great excursions.

For more information and details of how to register please visit our
web-site at

www.med.ic.ac.uk/external/ichc_2000

Student bursaries are still available!

DON'T MISS OUT IT WILL BE ONE OF THE CONFERENCES OF THE YEAR

Gary Coulton

Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

tel 0044 (0)171 594 3190
fax 0044 (0)171 594 3022

PLEASE NOTE AS FROM APRIL 1ST 2000 THE FOLLOWING NUMBERS WILL REPLACE THOSE
ABOVE AND SHOULD BE USED.

TEL. ++44 020 7594 3190
FAX. ++44 020 7594 3022
e-mail g.coulton-at-ic.ac.uk


-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Understanding Biocomplexity: The Post-Genome Challenge"

September 3-8, 2000, York, United Kingdom

ICHC 2000 will comprise 27 symposia addressing the latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

On-line registration now open!!!!!!!!!!

For further details of the meeting and how to pre-register please visit our
web-site at http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you there.



From daemon Wed May 03 08:37:18 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Wed, 03 May 2000 09:22:48 -0400
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Richard E. Edelmann" wrote:

} O.k., whereas not directly a microscopy question I was hoping some one would
} have a solution. Look for something that will function like aluminium foil but be non-
} reflective.
}
} (1) shapable around objects (we're covering petri dishes), but not too massive
}
} (2) be opaque (visble light 420-720nm)
}
} (3) be non-reflective on at least one side. "Non-reflective": less than 15%
} reflectivity to visible spectrum 420-720nm.
}
} Any suggestions? (Any good ideas for turning Al foil nice and black?)
}
} Thanks.
}

I seem to remember that studio photographers use flat black aluminum foil. Try Porter's
Camera, I think they are in Iowa. They are a large mail-order firm and probably have a
website. Or try Calumet camera, 800 Supreme Drive, Bensenville, IL 1-800-225-8638.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed May 03 08:37:18 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 3 May 2000 09:17:58 -0400
Subject: RE: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The best stuff that I ever found for mounting pictures is 3M Positionable
Mounting Adhesive. This is very good stuff and not messy at all. It comes
in rolls and is a transfer type material. You roll it out, place your
picture on it and cut it out with a sharp knife. You then press the
adhesive with the backing sheet and peel off the sheet. The adhesive is
transferred to every square inch of the print. You can position this stuff
around and then you press it down hard and it sticks forever.
The roll that I use is PMA 568. There is larger and smaller sizes
available. I think that you have to order through a photographic supply
house because I don't think any of the EM supply houses carry it.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
} Sent: Tuesday, May 02, 2000 7:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: dry mounting dye sub prints
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Does anyone have a good, safe way of mounting Codonics dye
} sublimation prints onto poster boards? We would like to avoid the
} spray on "rubber cement" types of glues due to the mess.
}
} Thank you.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}



From daemon Wed May 03 08:37:18 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 3 May 2000 09:25:09 -0400 (EDT)
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Matte black spray paint?

Tamara Howard
CSHL

On Wed, 3 May 2000, Richard E. Edelmann wrote:

}
} O.k., whereas not directly a microscopy question I was hoping some one would
} have a solution. Look for something that will function like aluminium foil but be non-
} reflective.
}
} (1) shapable around objects (we're covering petri dishes), but not too massive
}
} (2) be opaque (visble light 420-720nm)
}
} (3) be non-reflective on at least one side. "Non-reflective": less than 15%
} reflectivity to visible spectrum 420-720nm.
}
}
} Any suggestions? (Any good ideas for turning Al foil nice and black?)
}
} Thanks.
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}



From daemon Wed May 03 18:40:50 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 3 May 2000 08:48:54 -0500
Subject: Re: LM: Stains for polymers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


As to pyramitomes, if anyone has one that is not in use and would like to sell it, please contact me. I used one years ago and wouldn't mind having one around.
Debby


Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------




Greetings,
Babara Foster asked about staining polymers. By chance, I
recently looked at paper on staining polymers for electron
microscopy. The paper compared osmium and ruthenium tetroxides and
concluded that ruthenenium tet stained many more polymers than did
the osmium. This paper has a kind of encylopedic feel to it because
they checked a lot of polymers. The citation is Trent et al., 1983
Macromolecules 16: 589-598. I have no idea whether this would work
with light microscopy or what info you could get from it exactly
besides contrast, but perhaps you will find the paper interesting.

As ever,
Tobias
}
} Hi,
}
} I have been reading a great deal on polymer microscopy lately and came
} across the statement that there was very little information to be gained by
} staining polymers and (as might be expected) that they did not take up
} stain well. Many years ago, I had heard of an application in which osmium
} tetroxide was used, I believe on polyethylene. Can anyone shed any light,
} either on the general comment or on the application using osmium tetroxide?
}
} Many thanks,
} Barbara Foster,President
} Microscopy/Microscopy Education
} 125 Paridon Street Suite 102
} Springfield, MA 01118-2130
} PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
} Website: www.MME-Microscopy.com/education
}
} America's first national consortium of microscopy specialists offering
} customized on-site training in all areas of microscopy, image analysis, and
} sample preparation
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed May 03 18:40:50 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 3 May 2000 08:59:33 -0500
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
We got a bolt of black cloth from a theatrical supply house.
It is the kind of thing used to make curtains and other masking on
the stage. I think it was called "covert black" but I could have that
wrong. We were doing photobiological work and were forever having to
limit the pesky wandering photons. The cloth is thick but not too
thick to wrap around a dish. It is not perfectly opaque but if you
need that I bet you could line the cloth with al foil, on the inside.
Hope this helps,
Tobias
}
}
} O.k., whereas not directly a microscopy question I was hoping
} some one would
} have a solution. Look for something that will function like
} aluminium foil but be non-
} reflective.
}
} (1) shapable around objects (we're covering petri dishes),
} but not too massive
}
} (2) be opaque (visble light 420-720nm)
}
} (3) be non-reflective on at least one side.
} "Non-reflective": less than 15%
} reflectivity to visible spectrum 420-720nm.
}
}
} Any suggestions? (Any good ideas for turning Al foil nice and black?)
}
} Thanks.
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed May 03 18:40:51 2000



From: wft03-at-health.state.ny.us
Date: Wed, 3 May 2000 10:12:38 -0400
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





O.k., whereas not directly a microscopy question I was hoping some one
would
have a solution. Look for something that will function like aluminium foil
but be non-
reflective.

(1) shapable around objects (we're covering petri dishes), but not too
massive

(2) be opaque (visble light 420-720nm)

(3) be non-reflective on at least one side. "Non-reflective": less
than 15%
reflectivity to visible spectrum 420-720nm.


Any suggestions? (Any good ideas for turning Al foil nice and black?)




Dear Richard,

How about depositing carbon black onto Al foil? It's messy, but
fulfills

your requirements. Evaporating carbon seems like an inferior proceedure,
but

that should also work. Just holding the foil above a low-temp flame
(candle or

bunsen burner with the air turned down) seems the simplest. You can then

cover the carbon layer with something if necessary. Dipping the foil into
laser-

printer toner could also work, and the toner could then be heated. Putting
the

foil (cut to 8.5" x 11") through the printer is asking for trouble, but
that could

also work if you can find a solid black character--WordPerfect 5.1 has such

a character, which can be accessed by ctrl-v, 3,3. Good luck.

Yours,

Bill Tivol




From daemon Wed May 03 18:40:54 2000



From: Neal Leddy :      nleddy-at-tcd.ie
Date: Wed, 3 May 2000 15:39:55 +0100
Subject: TEM: Immunogold labelling protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am looking for a protocol for Immunogold labelling membrane proteins on
mitochondria / peroxisomes fractions..., I am hoping to use a formaldehyde
type fixation..............to LR white resin, I would appreciate any advice,
references, and if possible actual relevant protocols. Thank you in
advance.

Neal Leddy nleddy-at-tcd.ie



From daemon Wed May 03 18:40:56 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 03 May 2000 08:52:31 -0700
Subject: Re: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,
Years ago I purchased "Mounting Film" from an EM supply house. This is
double-sided sticky tape, two feet wide. You cut it to any size and press
on. Mine is made by Lomacoll and is obviously German.
At 06:58 PM 5/2/00 -0500, you wrote:
}
} Does anyone have a good, safe way of mounting Codonics dye
} sublimation prints onto poster boards? We would like to avoid the
} spray on "rubber cement" types of glues due to the mess.
}
} Thank you.
}
Best regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 03 18:40:56 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Wed, 3 May 2000 11:13:31 -0500 (CDT)
Subject: Re:Mounting Dye-Subs

Contents Retrieved from Microscopy Listserver Archives
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We've used DryBond Adhesive Pads for mounting dye-sublimation prints.
It's manufactured by Chartpak (their part number is DBS25 for 25 11" x 17"
sheets). I order it from EMS (Cat. #77612-25), described on Page 311 of
EMS Catalog XIII. Basically it consists of an array of tiny (well,
actually quite large to electron microscopists) dots of rubber cement like
stuff that you apply by placing the print on top, covering with a
protective sheet then rubbing with a rubber brayer. Peel up the print,
place on mounting board, cover with sheet and rub with brayer. We've
used it on color dye-subs with and without the XtraLife coating with no
problems. As far as longevity, I made a poster two years ago that is
still adhered tight to the board, but that was with RC B&W paper. The
oldest dye-sub has only been observed for about a year now.

I have no financial interest in Chartpak or EMS, the stuff is just nice to
use.

Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Wed May 03 18:40:59 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Wed, 03 May 2000 13:00:17 -0500
Subject: Re: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

I use a product called Liquid Paper Dryline Permanent Adhesive made by the
Gillette Company. It is supposedly acid free, glues instantly, no drying
time, and comes as either permanent or temporary. I get it a office supply
store.



} Does anyone have a good, safe way of mounting Codonics dye sublimation
} prints onto poster boards? We would like to avoid the spray on "rubber
} cement" types of glues due to the mess.

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Wed May 03 18:41:03 2000



From: Smartech :      smartech-at-javanet.com
Date: Wed, 3 May 2000 15:23:00 -0400
Subject: Digital Light microscopy, Kodak MDS 120, How does it compare?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to buy an inspection scope from which I can also acquire digital
images.

I remembered that I had a flyer for the Kodak MDS 120 system ($1895).

I called Kodak, they still sell the same system. It seems a little "out
dated" now w/ only 1.2 pixels and no USB connection.

Are there other product that offer similar simplicity and versatility
(macroscopic off the scope capability), that also fall in the same price
range, and have 2 million pixels w/ a USB connection?

Thanks in Advance

Ric



From daemon Wed May 03 18:41:09 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Wed, 3 May 2000 16:20:39 -0500 (CDT)
Subject: MICROSCOPY AND MICROANALYSIS 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Now that the program for the meeting is available on the website - Thanks Nestor
- I would like to advertize the "LATE-BREAKING POSTER SESSION AT MICROSCOPY AND
MICROANALYSIS 2000" for any remaining authors (or potential authors) who would
like to contribute to the meeting.

Microscopy and Microanalysis 2000 will feature a poster session composed of
presentations of newly acquired data or analyses which were unavailable for
submission by the February 15 deadline. A short, half page abstract describing
the studies is required. The abstract should include: Title, Authors, Authors
affiliation, and a Brief Description of the studies. The description should
include the Aim of the studies, a short characterization of the Methods, and a
brief account of the Results and their Importance.

Abstracts should be e-mailed or faxed to the program chair, Stuart McKernan, at
stuartm-at-tc.umn.edu (email) or 612-625-5368 (fax). Abstracts may be submitted
immediately but must be received by June 23, 2000. Abstracts will be reviewed by
members of the program committee. A limited number of poster boards are
available and preference will be given to early submissions. Abstract authors
will be notified of acceptance of their abstracts no later than July 1 (earlier
for early submissions).



__________________
Stuart McKernan stuartm-at-tc.umn.edu
Director Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368



From daemon Wed May 03 19:17:49 2000



From: Barry Searle :      B.Searle-at-unsw.edu.au
Date: Wed, 3 May 2000 18:42:02 -0500
Subject: JEOL COATER

Contents Retrieved from Microscopy Listserver Archives
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Can someone on the listserver offer any help, I am seeking the JEOL
publication of the list of O Rings (part numbers, or size/type) that are
used in the JEOL COATER JEE-400 and the JEE 4B/4C. If it is available
could you please either email me a copy of Fax me a copy Fax Number is
(in australia) +61 (2) 9385 6400. Thankyou for your help, Barry EM
UNIT UNSW




From daemon Wed May 03 19:17:49 2000



From: Tom Doman :      jtd1-at-psu.edu
Date: Wed, 3 May 2000 18:48:01 -0500
Subject: virus particle concentration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




My question is : I need to obtain a estimate of virus particle
concentration in a sample of cell culture supernatant for an article to be
submitted to a journal. I was told of a method called the 'latex particle
reference method' for obtaining virus particle counts. Can anyone on the
list supply information for this procedures ?
Thanks for your time.

Sincerely,

Tom Doman, Project Associate
Veterinary Science Department
Penn State University




From daemon Thu May 04 07:58:12 2000



From: Marilyn Henderson :      marilyn.henderson-at-adelaide.edu.au
Date: Thu, 04 May 2000 10:20:43 +0930
Subject: Re: TEM: Immunogold labelling protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Neal,
A good fixative for immunogold work is still a combination of glut. +
paraformaldehyde, but using a 'low' glutaraldehyde concentration. One
you could try would be 0.25% glutaraldehyde + 4% paraformaldehyde in
phosphate buffered saline pH 7.2. Formaldehyde alone results in poor
structural preservation.
From there on you could follow the instructions for dehydration,
infiltration etc. from the LR White pamphlet which works well.
For labelling:
1. Pre-treat with 0.02M glycine in PBS to block any free aldehyde groups
(2x10mins), 2. Block with a suitable protein, e.g. BSA, ovalbuimin (1%-
1X20mins)
3. Place each grid on a 15-20ul drop of primary antibody, suitably
diluted, and either leave overnight at 4 degrees C in fridge, or on lab
bench for about 2 hours.
4. Rinse 6 times in drops of PBS with 1% protein block (BSA etc.) -
(6X5mins).
5. Place each grid on a 20ul drop of Protein-A Gold complex, suitably
diluted, for 1-1.5 hours.
6. Rinse in PBS (6X5mins).
7. Wash in distilled or millipore filtered water (in small beakers, 3 x
several rapid dips(about 6), making sure the grids remain under the
water at all times during dipping).
8. Stain with Uranyl Acetate and Lead Citrate.

** Blot (side-on) onto filter paper between steps 1+2, 2+3, 4+5, 7+8.

Good luck.

Cheers,
Marilyn

Neal Leddy wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
}
} I am looking for a protocol for Immunogold labelling membrane proteins on
} mitochondria / peroxisomes fractions..., I am hoping to use a formaldehyde
} type fixation..............to LR white resin, I would appreciate any advice,
} references, and if possible actual relevant protocols. Thank you in
} advance.
}
} Neal Leddy nleddy-at-tcd.ie



From daemon Thu May 04 07:58:15 2000



From: Jeff & Wanda Gray :      jefwan-at-flash.net
Date: Wed, 3 May 2000 21:02:31 -0500
Subject: Job Opening at Univ of Houston

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please contact the person at the end of the post, not me, as I am posting at
the request of someone without access. Thank you.
----- Original Message -----
Sent: Wednesday, May 03, 2000 8:59 PM


} JOB OPORTUNITY AT UNIVERSITY OF HOUSTON
}
}
} Supervisor: Histology and Microscopy Laboratory; College of Optometry;
} University of Houston, Texas
}
} Job Description:
}
} This position is in direct support of research and teaching faculty
} where the subject of interest is typically ocular and neural tissue. The
} duties range from tissue preparation, embedment, sectioning and
} mounting, to operation of light and transmission electron microscopes,
} and development and printing of photographic results. Knowledge and
} competency in operation of ultramicrotomes and the transmission electron
} microscope as well as current techniques in morphology, histology, and
} immunocytochemistry is required. Experience with cryo-microscopy, in
} situ hybridization, and computer image analysis is desirable. The
} successful candidate also may participate as co-author of research
} papers describing research performed in the laboratory.
}
} The supervisor is responsible for maintenance of stocks of laboratory
} supplies and care of the equipment, and keeping the lab clean and
} usable, including proper disposal procedures for hazardous wastes. Other
} duties include instructing graduate students in common and specialized
} anatomical techniques required for their various projects, and
} supervision and consultation on their work as required. Some effort also
} is applied to teaching of undergraduate optometry students including
} preparation of teaching slides and technical instruction in anatomical
} methods.
}
} This job is scheduled to begin on 1 May 1999. Salary will be negotiated
} based upon qualifications and experience. For further information, you
} may contact:
}
} Chris Kuether, Technical Services Manager
} College Of Optometry, University Of Houston
} 4901 Calhoun Blvd. Houston TX 77204-6052
} vox:(713)743-2049 fax:--2053; ckuether-at-uh.edu
}
}
}



From daemon Thu May 04 07:58:20 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 4 May 2000 14:01:16 +1000
Subject: RE: SEM Peltier stage vendors?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can read up on Emitech cold stages on our site
home} contents} K4
We are agents for Emitech for Australasia only (south of Singapore), just hope
that you would find the info useful.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, May 02, 2000 11:37 PM, nessler [SMTP:randy-nessler-at-uiowa.edu]
wrote:

} We are looking for vendors of Peltier cooling stages for our Hitachi
} 2460N VPSEM. If you know of a company, please drop me a line. Also, if
} you have opinions on what features you would look for in such an item,
} I'd like to hear it.
} Thanks in advance,
} Randy
} --
} Randy Nessler
} Views expressed are my own.



From daemon Thu May 04 07:58:38 2000



From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 04 May 2000 08:28:27 -0400
Subject: Re: virus particle concentration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have archived three discussions on this from the listserver. Go to:

http://www.biotech.ufl.edu/~emcl

and look in the TEM section of "Tips & Tricks" under virus. It also has a
few other ideas. Good luck


At 06:48 PM 5/3/2000 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
EM Technician Gainesville, FL 32610
University Of Florida ph 352-392-1184
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "







From daemon Thu May 04 07:58:38 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Thu, 4 May 2000 08:37:54 -0400
Subject: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I think that I have used something that would work well for your
application. It is an anodized Al foil that we use for laser safety
purposes. The foil is very flat black and the black does not rub off
easily. However, the foil is significantly stiffer than household Al foil.
The brand we have used is BlackwrapTM and it can be purchased from The
Great American Market, Hollywood CA 323-461-0200. They have a web site as
well. I hope that this helps.

Matthew Ervin, Ph.D.
U.S. Army Research Laboratory
(301)394-0017
MErvin-at-ARL.mil




"Richard E. Edelmann" {edelmare-at-casmail.muohio.edu} on 05/03/2000 07:39:20
AM

Please respond to Edelmare-at-muohio.edu

To: microscopy-at-sparc5.microscopy.com
cc:


O.k., whereas not directly a microscopy question I was hoping some one
would
have a solution. Look for something that will function like aluminium foil
but be non-
reflective.

(1) shapable around objects (we're covering petri dishes), but not too
massive

(2) be opaque (visble light 420-720nm)

(3) be non-reflective on at least one side. "Non-reflective": less
than 15%
reflectivity to visible spectrum 420-720nm.


Any suggestions? (Any good ideas for turning Al foil nice and black?)

Thanks.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."









From daemon Thu May 04 08:10:55 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 4 May 2000 07:59:21 -0500
Subject: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Does anyone currently use the Sony DKC-5000 CatsEye Digital Camera
and if
so would you recommend it for a shared technology lab.
Rosemary Walsh, EM Facility for the Life Sciences
Penn State University




From daemon Thu May 04 10:12:35 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Thu, 4 May 2000 08:17:30 -0500
Subject: RE: Stains for polymers?

Contents Retrieved from Microscopy Listserver Archives
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Barbara,
We use both osmium and ruthenium tetroxide to stain various polymers for EM
observation. Ruthenium is less selective in its staining, and therefore
does stain many more polymers. Both compounds stain unsaturated polymers
pretty well. In olefins, like polyethylene, RuO4 is good for staining
amorphous domains within the crystalline or semi-crystalline polymer fine
structure. This is typically a TEM issue. With some rather coarse polymer
blends (e.g. rubber inclusions in another polymer matrix) RuO4 or OsO4 can
be used with SEM and FESEM imaging. I would expect to see very little
staining contrast by LM due to the polymer domain sizes of typical
co-polymers, polymer blends, or crystalline/amorphous polymer structure.
Again the best reference I have is the book by L. Sawyer, Polymer
Microscopy.

Brad Huggins
BP Amoco, Naperville, IL

} ----------
} From: Barbara Foster[SMTP:mme-at-map.com]
} Sent: Tuesday, May 02, 2000 4:57 PM
} To: Confocal Microscopy List; microscopy-at-sparc5.microscopy.com
} Subject: LM: Stains for polymers?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I have been reading a great deal on polymer microscopy lately and came
} across the statement that there was very little information to be gained
} by
} staining polymers and (as might be expected) that they did not take up
} stain well. Many years ago, I had heard of an application in which osmium
} tetroxide was used, I believe on polyethylene. Can anyone shed any
} light,
} either on the general comment or on the application using osmium
} tetroxide?
}
} Many thanks,
} Barbara Foster,President
} Microscopy/Microscopy Education
} 125 Paridon Street Suite 102
} Springfield, MA 01118-2130
} PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
} Website: www.MME-Microscopy.com/education
}
} America's first national consortium of microscopy specialists offering
} customized on-site training in all areas of microscopy, image analysis,
} and
} sample preparation
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} ^^
}



From daemon Thu May 04 10:12:37 2000



From: David Kinast :      DKinast-at-hitschfel.com
Date: Thu, 04 May 2000 09:29:04 -0500
Subject: Re: Digital Light microscopy, Kodak MDS 120, How does it

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

We use routinely this method in our laboratory. Here is the used protocol of
quantitation of virus particles by negative stain electron microscopy:


A selected volume (100 uL) of supernatant containing virus particles is
mixed with a selected volume (100 uL) of polystyrene latex beads of known
concentration (about 10e8 beads by mL) and a size between 100 - 200 nm in
diameter.
The mixture is placed in an Beckman Airfuge 240 µL-tube. A Formvar and
carbon-coated grid is inserted into the bottom of the microtubes.* The tubes
are placed in a Airfuge A-100 fixed angle rotor (30¡) and centrifuge at 20
psi (120 000 g) for 5 minutes.
The grids are recovered with fine self-closing tweezers, dried with bibulous
paper, stained 1 minute with phosphotungstic acid (PTA 3%, pH6.0) and dried
again with bibulous paper. Samples are visualized under a transmission
electron microscope with an approppriate magnification.
On two different grids, 200-500 particles (latex beads or virus particles)
are counted from at least five different areas on each grid. That way, from
the ratio of the two types of particles in the suspension, the ratio of the
volumes added and the known concentration of latex particles, the
concentration of viral particles can be calculated.

Virus particle concentration (particles/mL) = (virus count / latex beads
count) X (latex beads concentration) X (1/test article dilution)


The level of sensitivity of this procedure is between 10e6 and 10e10
particles per mL. Less than 10e6, there is not enough virus to get a
realistic count and more than 10e10, there are too much virus to well
differentiate them.
This procedure is used principally to quantify Retrovirus type-A and -C
particles in cells supernatant, but can be used for any virus particles.

*R. Alain et al, J.Virol. Meths, 16 (1987), 209-216
*Alain, R. Microscopy Today, May 1997, issue#97-4, D. Grimes Ed, p. 20

If you need more explanation you can contact us. If you are not the
equipment to do this technique, we can offer this service at low price.

Robert Alain

**********************************************************
Robert Alain, M.Sc.
Microscopie Žlectronique

INRS-Institut Armand-Frappier
Centre de Microbiologie et Biotechnologie
531 boul. des Prairies
Laval, QuŽbec
CANADA H7V 4B7
Tel: (450)687-5010 ext#4388
Fax: (450)686-5626
e-mail: Robert.alain-at-INRS-iaf.uquebec.ca
or Robert_alain-at-hotmail.com
Http://www.iaf.uquebec.ca/iaf/recherche/viro/me.html
**********************************************************

----- Original Message -----
} From: Tom Doman {jtd1-at-psu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 03, 2000 7:48 PM


{html}
You may want to look at the Olympus DP-11 digital camera. You can find it
on their web page at:
{a href="http:///"} http://www.olympusamerica.com/product.asp?c=21&s=11&p=18&product=612 {/a} {br}
It has very high resolution, live video output (which is quite useful at
low magnification as a focusing and framing aid) and features a c-mount
so that it can be used on any microscope, or with a macro lens.  It
uses SmartMedia cards as the recording media, and combined with a USB
SmartMedia card reader, download to the computer is fast and easy. {br}
{br}
Configured with an AC adapter, 64MB SmartMedia card, USB card reader and
a 9" Sony video monitor, it is priced at $4,349.00.  This is
more than you indicated you would like to spend, but I think that you
will find that its features, resolution and ease of use may justify the
additional expense. {br}
{br}
At 03:23 PM 5/3/00 -0400, you wrote: {br}
>------------------------------------------------------------------------ {br}
>The Microscopy ListServer -- Sponsor: The Microscopy Society of
America {br}

{/html}



From daemon Thu May 04 16:31:00 2000



From: Richard E. Edelmann :      edelmare-at-casmail.muohio.edu
Date: Thu, 4 May 2000 11:35:05 -0500
Subject: Thanks on the non-reflective and EELS humor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to everyone who responded - I was thinking anodizing myself but Matt
Ervin actually had an Off-the-Shelf source.

===============

Its exam time here and I wanted to share this answer from my EM Theory Final:

Ques: What does "EELS" stand for? What does it detect (Be specific!)?

Ansr: Energized Eucaliptic Leaf Shooter. If properly used it can be used to lure the
better part of the world's Koala Bear population in a general area to get a more
precise population density reading.

[The student recieved 1 point for creativity]



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Thu May 04 16:31:01 2000



From: David Kinast :      DKinast-at-hitschfel.com
Date: Thu, 04 May 2000 08:39:20 -0500
Subject: LM product specialist needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html}
{br}
{br}
{font size=6} {b} {div align="center"}
SALES POSITION AVAILABLE {br}
{br}
{br}
{/font} {/b} {font size=5} {/div}
Hitschfel Instruments, Incorporated is a consultative sales company,
providing microscope based imaging solutions to the biomedical research,
clinical and industrial communities within the states of Missouri,
Nebraska, Kansas and Oklahoma plus central & southern Illinois. {br}
{br}
We are seeking one exceptional and highly motivated individual to work
with us toward our mutual goals of success through customer
satisfaction.Ê A strong science background coupled with experience in the
fields of microscopy and digital-imaging technology is required.Ê If you
are the right person for the job, we will teach you the selling skills
that you will need to be successful. {br}
{br}
If you are looking for a lifelong career in scientific instrument sales,
please submit your resume to: {br}
{br}
{div align="center"}
Hitschfel Instruments, Incorporated {br}
2333 South Hanley Road {br}
St. Louis, MO 63144 {br}
Attn: David Kinast {br}
FAX: 314/644-5877 {br}
{/font} {font size=4 color="#0000FF"} {u} dkinast-at-hitschfel.com {br}
{a href="http://www.hitschfel.com/" eudora="autourl"} www.hitschfel.com {br}
{/a} {/font} {/u}
{BR}
{div} David L. Kinast {/div}
{div} Hitschfel Instruments, Inc. {/div}
{div} 2333 South Hanley Rd. {/div}
{div} St. Louis, MO 63144 {/div}
{div} Phone: {x-tab}    {/x-tab} 800/242-3501 {/div}
{div} Phone: {x-tab}    {/x-tab} 314/644-6660 {/div}
{div} Fax: {x-tab}      {/x-tab} {x-tab}     {/x-tab} 314/644-5877 {/div}
{div}
{a href="http://www.hitschfel.com/" EUDORA=AUTOURL} www.hitschfel.com {/a} {/div}
{div} dkinast-at-hitschfel.com {/div}
{/html}

{/html}



From daemon Thu May 04 16:31:04 2000



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Thu, 04 May 2000 11:10:20 -0700
Subject: CCL Supplier Information Request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers-
I am hoping that someone might have a current address or phone for
"Cambride Image Technologies Ltd." or "Nuclide" A colleague of mine is
looking to purchase a cold cathode luminoscope and was given these two
names. Or if someone knows of another supplier any information would be
appreciated.
Thank you,
Sarah

************************************************************************

Sarah A.W. Lundberg Lab
(EPMA) (702) 895-2660 or
Electron Microanalysis and
(SEM) (702) 895-2462
Imaging Laboratory
Office (702) 895-1134
Department of Geoscience, UNLV Fax
(702) 895-4064
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010 Dept Office
(702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635
************************************************************************





From daemon Thu May 04 16:31:04 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Thu, 04 May 2000 11:17:05 -0700
Subject: "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe that what Geoff was referring to is called "Black Wrap." It is a
heavy foil with a matte black coating. It is used by theater lighting
people worldwide to create custom masks and light shields. It is made by
several companies. My local lighting house sells black wrap made by Great
American ... in 12 inch by 50 foot or 24 inch by 25 foot rolls for
US$27.50. Since you're at a university you could probably go over to the
theater department and get a little piece to try.

Larry


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Thu May 04 16:31:08 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 04 May 2000 14:19:48 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 05:59 AM 5/4/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

After some time of getting used to it, the 5000 is OK. It uses
a small CCD so the image files are small and not really high
resolution. But for relatively modest final image output size,
it is a good camera. It provides real time focusing via a
separate RGB+sync color monitor. However, adjusting the
monitor to match the captured image's exposure is tricky.

If you are going to be moving the camera around, remember
that it uses a SCSI interface to download captured images.
You won't be able to move it very far from the SCSI port
unless you use a notebook or laptop computer.

gary g.



From daemon Thu May 04 21:48:35 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Thu, 4 May 2000 19:28:11 -0500
Subject: Mounting tape for posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Scotch 924 Transfer Tape 1/2 inch wide is the product I mentioned
earlier. It cost about 6.95 a roll for a life time supply for many of us.
http://productinfo.mmm.com/us/office/products/office.jhtml?powurl=4JLD1SMBbe
ZCZYKRQ9geT1T4S9TCgvPDJGVQ33gl

The way I used it to mount photos was stick a band of it around the
edge of the photo as close as I could to the edge. That's all I did
for 5X7's for 8X10 I put a cross diagonally from corner to corner.
I didn't mount prints larger than that but If I had I would have added
some tape in the open areas. Some one made a small version
that had an applicator but the refills cost as much as a full sized roll
and it is not much if any easier to use.

I tried a lot of things spray glue, two sided tissue and a number of
others. This was the easiest and most consistent other than Seal
mount tissue and a hot press.

The hardware store where I drink coffee is will sell mail order. I have
not interest in the hardware store other than a bunch of us drink their
coffee. Their web page address is www.studyboards.com or 405 372 2644
ask of Sandy or A.J.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri May 05 07:45:33 2000



From: Udo Graf :      udo.graf-at-bal-tec.com
Date: Fri, 5 May 2000 08:38:51 +0200
Subject: WG: SEM Peltier stage vendors?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



You can find information on www.bal-tec.com --} products--} vct100

Best regards,
Udo Graf
BAL-TEC AG
+423 388 12 26
+423 388 12 60 (Fax)
udo.graf-at-bal-tec.com



+ -----Ursprüngliche Nachricht-----
+ Von: nessler [mailto:randy-nessler-at-uiowa.edu]
+ Gesendet: Dienstag, 2. Mai 2000 14:37
+ An: miscroscopy listserver
+ Betreff: SEM Peltier stage vendors?
+
+
+ We are looking for vendors of Peltier cooling stages
+ for our Hitachi
+ 2460N VPSEM. If you know of a company, please drop me a
+ line. Also, if
+ you have opinions on what features you would look for in
+ such an item,
+ I'd like to hear it.
+ Thanks in advance,
+ Randy
+ --
+ Randy Nessler
+ Views expressed are my own.
+



From daemon Sat May 06 20:54:25 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 5 May 2000 17:09:03 +0200
Subject: Fossil Replicas for SEM inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have customer here requiring some help. They are making replicas of fossil
mandibles for SEM analysis. Their problem is how to localise the areas of
interest when the specimen is in the SEM. I was sent the quotation below,
and we are trying to find out whether we, or anyone else, can supply the
sort of material they need. My background is mainly materials, so I'm
farming this out to the list to see if anyone has any suggestions, or maybe
even recognises the fragment below.


"Fiberglas screening material:
fiber thickness (310 microns), hole width (1,
240 microns), and length (1,340 microns), wiped with Permabond 910 adhesive
and pressed onto the surface so that contact was made everywhere".


Thanks in advance

Tim

*****************************************************************
Tim E. Harper Managing Director
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/ {http://www.cmp-cientifica.com/}



From daemon Sat May 06 20:54:27 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 05 May 2000 11:20:44 -0500
Subject: Re: Fossil Replicas for SEM inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That sounds a lot like fiberglass window screen to me, but the holes here
are probably a bit smaller. There is also a fiberglass screening used for
backing plaster repairs. It might have a pitch more similar to what you
described.

At 05:09 PM 5/5/2000 +0200, you wrote:
} Hi,
}
} I have customer here requiring some help. They are making replicas of fossil
} mandibles for SEM analysis. Their problem is how to localise the areas of
} interest when the specimen is in the SEM. I was sent the quotation below,
} and we are trying to find out whether we, or anyone else, can supply the
} sort of material they need. My background is mainly materials, so I'm
} farming this out to the list to see if anyone has any suggestions, or maybe
} even recognises the fragment below.
}
}
} "Fiberglas screening material:
} fiber thickness (310 microns), hole width (1,
} 240 microns), and length (1,340 microns), wiped with Permabond 910 adhesive
} and pressed onto the surface so that contact was made everywhere".
}
}
} Thanks in advance
}
} Tim
}
} *****************************************************************
} Tim E. Harper Managing Director
} CMP Cientifica s.l.
} Space & NanoTechnology Division
} Phone +34 91 640 71 85 Fax +34 91 640 71 86
} http://www.cmp-cientifica.com/ {http://www.cmp-cientifica.com/}



From daemon Sat May 06 20:54:27 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Fri, 05 May 2000 09:57:37 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had the Sony DKC-5000 for quite a few years and hve never had a
problem with the hardware despite many clumsy users. We have it running on
a Mac computer taking the Sony RGB signal to SVHS via a Harmonic Research
CV-233P video encoder. The SVHS signal is input to a ATI Xclaim video board
in the Mac. So the live window and the Sony acquire plug in run on the same
monitor. The density and color balance rarely coincide between the two
displays. We have had many problems with users changing settings and adding
software, etc. on the Mac resulting in problems with the video display. As
Dr Gaugler said, the resolution is not very high. There should be better
systems now but I continue to recommend that our scientists record their
images on color slide film and then scan it with a $2000 slide scanner
(Nikon, Polaroid etc.) much cheaper, higher resolution and bandwidth and
easy convenient storage!





Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Sat May 06 20:54:28 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 5 May 2000 12:22:45 -0500
Subject: low temperature microscopy of synthetic fluids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are still not set-up well for Cryo-SEM, and although we have made some
progress and learned much about these techniques from you all on this
Listserver and others, I am still looking for a "cryomicroscopy facility"
who has, for example, an E-SEM with Peltier Stage/controlled cooling rate
capabilities and with cryostage (capable down to temperatures as low as
approximately -75C); who would be interested in working together with one of
my clients, in a large Chemical/Oil Company, to investigate/observe the
low-temperature structures of various Synthetic Fluids.

Anyone interested, please contact:
Brad Huggins at
BPAmoco, Naperville, IL
hugginbj-at-bp.com
630 420-3668



From daemon Sat May 06 20:54:43 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Fri, 05 May 2000 15:46:56 -0700
Subject: silver enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
Does anyone have a good protocol for silver enhancement of immunogold
labeled sections for TEM? I have a paper in front of me, but they used a
kit. We do light level silver enhancement without a kit all of the time,
but I don't want to chance trying to adapt that method before consulting
all of you.
Thanks for your help!
Have a wonderful weekend,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu
909-787-4525



From daemon Sat May 06 20:54:45 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 05 May 2000 15:53:50 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If I understand correctly how you are using the DKC-5000,
then you are actually obtaining half the image resolution
that the system can provide.

The DKC-5000 has a 1/2" 440,000 pixel CCD with an
effective image area of 795x598 pixels (RGB). The
actual captured image area is probably closer to 740x580
pixels (RGB). Thus, if my calculations are correct, you
should obtain RGB TIF files which are about 1.29MB in
size.

The image processor in the DKC-5000 digital processor system
unit expands (interpolates?) the original image to one that is
1520x1144 pixels (RGB). This results in a TIF file that is
about 5.21MB in size. However, this resolution is only
obtained via the SCSI interface on the back of the
system box. This is likely why you are obtaining low
resolution results. This is effectively taking a consumer
grade RGB video camera and performing an RS-170
frame grab. This will produce up to 800x600 pixels (RGB)
maximum. This is OK, but the higher cost of the DKC-5000
was due, I think, to the ability to obtain higher quality
images and to do so without any separate/extra image
capture hardware. The DKC-5000 was about $14K I think
versus about $800 for a really nice RGB video camera
with close to 800x600 pixels resolution.

Therefore, if you have any sort of typical Mac, it should have
a legacy SCSI-I connector on the rear. This is easily
connected to the digital processor box. Then, load the
Photoshop plug-in and acquire the higher resolution images
using Photoshop and the SCSI bus. You should still be
able to use the RGB outputs for framing and focusing.
Just don't use the RGB outputs for image capture.

Have you tried this?

gary g.



At 09:57 AM 5/5/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 08 23:09:30 2000



From: A. Greene :      ablue-at-io.com
Date: Sun, 7 May 2000 12:15:37 -0500
Subject: Help finding parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers, I am looking for a couple decomissioned electron
microscopes to be used as a source for parts. I would greatly appreciate
any information or leads. The instruments are the Hitachi 7000 TEM and the
Hitachi S-510 (or 515 or 520) SEM. Thank you very much, Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone: 512/282-5507 Fax: 512/280-0702 QUALITY ELECTRON MICROSCOPE REPAIR




From daemon Mon May 08 23:09:30 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Sun, 7 May 2000 13:16:29 -0400
Subject: Mike Hales Contact Required

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Mike I need to contact you very urgent.

Could you mail me back please?

Steve Chapman
Senior Consultant Protrain



From daemon Mon May 08 23:09:32 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sun, 7 May 2000 05:06:02 -0700
Subject: Help with very old Leitz projection microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At http://www.couger.com/gcouger/leitz/ are some pictures of a
very old Leitz projection microscope. For it's age and rough
construction it works extreemly well. It is of cast iorn, steel
and brass construction and carries no serial number. It
has two objective lenses that are about 6 and 12 x It has
a simple condenser in the lamp housing and every thing
is adjustable on the stand and the objectives have a heilical
ajustment.

I have as several musem curators and infivigules that have
a life long assoitions wiht Leitz. No one has seen anything
like it. It appears to be all origianl except for the light bulb,
its mounting and the cord. They look like they came from
the 1920's

} From my decussions with Leitz sholers and deduction I think
this was made about the same time as the electric light bulb.
It would be possible that it use a gas or lime light but I don't
think so. The mechenism for centering a light bulb just looks
like it was designed for a bulb.

Any help is welcome. If anyone knows when Leitz started putting
serial numbers on their products it would be a great help in
dating it.

Thanks
Gordon Couger
Stillwater, OK 74075
405 624 2855 GMT - 6:00
www.couger.com/gcouger



From daemon Mon May 08 23:09:37 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sun, 7 May 2000 09:53:23 -0700
Subject: Re: Help with very old Leitz projection microscope (with corrected spelling)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am sorry the last post some how escaped my spell check
on my email program. It is set to check every email. But it
misses some. For Microsoft an occasional miss is pretty good:)

At http://www.couger.com/gcouger/leitz/ are some pictures of a
very old Leitz projection microscope. For it's age and rough
construction it works extremely well. It is of cast iron, steel
and brass construction and carries no serial number. It
has two objective lenses that are about 6 and 12 x It has
a simple condenser in the lamp housing and every thing
is adjustable on the stand and the objectives have a helical
adjustment.

I have as several museum curators and individuals that have
a life long association with Leitz. No one has seen anything
like it. It appears to be all original except for the light bulb,
its mounting and the cord. They look like they came from
the 1920's

} From my decisions with Leitz scholars and deduction I think
this was made about the same time as the electric light bulb.
It would be possible that it use a gas or lime light but I don't
think so. The mechanism for centering a light bulb just looks
like it was designed for a bulb.

Any help is welcome. If anyone knows when Leitz started putting
serial numbers on their products it would be a great help in
dating it.

Thanks
Gordon Couger
Stillwater, OK 74075
405 624 2855 GMT - 6:00
www.couger.com/gcouger






From daemon Mon May 08 23:09:49 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Mon, 8 May 2000 08:49:33 -0400
Subject: CCL Supplier Information Request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sarah-
I can't help you with Cambridge Image Technologies Ltd., but I do know
about Nuclide. Nuclide went bankrupt several years ago and was bought up
and reborn as Premier American Technologies Corp. which continued making
the luminoscopes among other things. I worked for PATCO for a couple of
years. I don't know the details, but PATCO has since evolved into Spectru
Medix and I imagine they still sell luminoscopes as it was probably their
most consistent seller in the past. You can call Spectru Medix at
814-867-8600, they are at 2124 Old Gatesburg Rd., State College, PA. I
would suggest you talk to Mike Vollero as I know he still works there and
will be able to put you in contact with the proper people. I hope this
helps.
Matt Ervin




Sarah Lundberg {lundberg-at-nevada.edu} on 05/04/2000 02:10:20 PM

To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
cc:


Hello listers-
I am hoping that someone might have a current address or phone for
"Cambride Image Technologies Ltd." or "Nuclide" A colleague of mine is
looking to purchase a cold cathode luminoscope and was given these two
names. Or if someone knows of another supplier any information would be
appreciated.
Thank you,
Sarah

************************************************************************

Sarah A.W. Lundberg Lab
(EPMA) (702) 895-2660 or
Electron Microanalysis and
(SEM) (702) 895-2462
Imaging Laboratory
Office (702) 895-1134
Department of Geoscience, UNLV Fax
(702) 895-4064
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010 Dept Office
(702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635
************************************************************************










From daemon Mon May 08 23:10:04 2000



From: Harry J. Grier :      harry.grier-at-dep.state.fl.us
Date: 5/8/00
Subject: Staining Spurr's resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have recently encountered some perplexing problems staining tissue
embedded in Spurr's resin with uranyl acetate and calcined lead. The
tissue is not taking up the stain although there is some stain
precipitate on the sections. Any suggestions?

Sincerely,
Harry Grier
Stock Enhancement Research Facility
Florida Marine Research Institute
14495 Harllee Road
Palmetto, FL 34221
harry.grier-at-fwc.state.fl.us



From daemon Mon May 08 23:10:05 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Mon, 08 May 2000 11:24:09 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
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Apparently I was not clear in my description of our DKC-5000 setup. The RGB
signal displayed in a live ATI video window is for focusing and composing.
Acqusitition of the image is via the Sony plug-in and SCSI transfer. The
resulting image file is about 5MB. It does not meet my standards for a
publication quality image.
Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Mon May 08 23:10:06 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Mon, 8 May 2000 15:10:46 -0400
Subject: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a question about how best to maintain a sputter coater that is used
infrequently.

We are a small college with correspondingly small EM facility. Our SEM and
prep equipment may go for several weeks without being used, then someone
will need it heavily for a class for three or four weeks. Invariably we
find that our sputter coater seems to be the weak link in our plans, since
it rarely works well when we fire it up after long periods of dormancy. I
understand this is common with vacuum equipment: better to use it often
rather than shut it off.

Am I correct that we should we have a plan to regularly pump down the
sputter coater, and if that is good idea, how often should that be? Every
day? Once a week? Once a month?

Or, if we don't need to pump it down regularly, are there other things we
should be doing do it the down time instead of letting it just sit there?

and finally, are some designs better able to handle long periods of disuse?
Do we just have the wrong sputter coater?

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice




From daemon Mon May 08 23:10:07 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 8 May 2000 15:56:43 -0500
Subject: See-Vac Inc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any old parts for the See-Vac, Inc. Autoconductavac
sputter coaters? Especially the power feed-through that connects
through the cap to the target. Thanks.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Mon May 08 23:10:08 2000



From: Chere Petty :      cpetty1-at-umbc.edu
Date: Mon, 08 May 2000 17:11:44 -0400
Subject: Job Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopist: To manage light and electron microscopy facility. Minimal
degree requirement BS (MS preferred). Experience with confocal
microscopy, computerized image analysis and histological sample
preparation essential. Experience in SEM/TEM sample preparation and
video microscopy desirable. Must be an interactive person willing to
facilitate microscopy experiments for faculty and students with a wide
variety of interests in a University setting. Salary commensurate with
experience. Full time desired but will consider part time. For further
information on the department see http://www.umbc.edu/biosci UMBC is an
AA/EOE.

Contact: Dr. Daphne Blumberg, Chair, Microscopist Search Committee,
Dept. of Biological Sciences, University of Maryland Baltimore County
(UMBC), Baltimore MD 21250.




From daemon Tue May 09 00:20:30 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 9 May 2000 14:09:53 +1000
Subject: RE: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All vacuum systems are better "stored" under vacuum, with the exception of any
systems that cannot vent the rotary pump when power is off. Those systems may
suck oil from the pump towards the vacuum chamber.

The more common problem for infrequently used systems is moisture in the pump
oil. Particularly in moist climates and when relatively short pumping times are
employed a good deal of water is absorbed in the pump. When the pump is not
used for a lengthy period this may cause corrosion and certainly lowers the
vapour pressure of the contaminated oil and so lower performance results.

I suggest that at the end of your spasmodic activities the pump should be run
with the baffle valve partially open for at least 30 minutes. The baffle valve
is usually atop the rotary pump. You could also use the sputter coater's needle
valve, partially open. Under these conditions the pump will run hotter and
throw-out a good deal of oil mist and water vapour. Vent the exhaust into a
fume hood since oil mist is not just unpleasant. You will find that the pump
performs much better after a baffle run.

SEM and TEM usually do not need this treatment, but they may, for instance if a
TEM has been used to "dry" film that was not previously dried in another
system.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, May 09, 2000 5:11 AM, Gary Radice [SMTP:gradice-at-richmond.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a question about how best to maintain a sputter coater that is used
} infrequently.
}
} We are a small college with correspondingly small EM facility. Our SEM and
} prep equipment may go for several weeks without being used, then someone
} will need it heavily for a class for three or four weeks. Invariably we
} find that our sputter coater seems to be the weak link in our plans, since
} it rarely works well when we fire it up after long periods of dormancy. I
} understand this is common with vacuum equipment: better to use it often
} rather than shut it off.
}
} Am I correct that we should we have a plan to regularly pump down the
} sputter coater, and if that is good idea, how often should that be? Every
} day? Once a week? Once a month?
}
} Or, if we don't need to pump it down regularly, are there other things we
} should be doing do it the down time instead of letting it just sit there?
}
} and finally, are some designs better able to handle long periods of disuse?
} Do we just have the wrong sputter coater?
}
} Gary P. Radice gradice-at-richmond.edu
} Associate Professor of Biology 804 289 8107 (voice)
} University of Richmond 804 289 8233 (FAX)
} Richmond VA 23173 http://www.science.richmond.edu/~radice
}
}



From daemon Tue May 09 07:20:55 2000



From: Udo Graf :      udo.graf-at-bal-tec.com
Date: Tue, 9 May 2000 09:01:53 +0200
Subject: AW: low temperature microscopy of synthetic fluids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The new BAL-TEC 'VCT 100' Vacuum-Cryo-Transfer-Equipment is a modular system
consisting of:

- Shuttle (Cryo-Vacuum Conditions)
- Docking station at any preparation system
- Docking station at any analysis system (SEM, ESEM, Cryo-AFM)
- Cryo equipment for any analysis system (SEM, ESEM)

The modules can be arranged just to meet your needs.

VCT 100 Cryo equipment has been adapted to a Philips XL30 e.g.

Additional information you will find on www.bal-tec.com --} products--} vct100

Best regards,
Udo Graf
BAL-TEC AG
+423 388 12 26
+423 388 12 60 (Fax)
udo.graf-at-bal-tec.com



-----Ursprüngliche Nachricht-----
Von: Huggins, Bradley J [mailto:HUGGINBJ-at-bp.com]
Gesendet: Freitag, 5. Mai 2000 19:23
An: Huggins, Brad; Microscopy listserver
Betreff: low temperature microscopy of synthetic fluids


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



We are still not set-up well for Cryo-SEM, and although we have made some
progress and learned much about these techniques from you all on this
Listserver and others, I am still looking for a "cryomicroscopy facility"
who has, for example, an E-SEM with Peltier Stage/controlled cooling rate
capabilities and with cryostage (capable down to temperatures as low as
approximately -75C); who would be interested in working together with one of
my clients, in a large Chemical/Oil Company, to investigate/observe the
low-temperature structures of various Synthetic Fluids.

Anyone interested, please contact:
Brad Huggins at
BPAmoco, Naperville, IL
hugginbj-at-bp.com
630 420-3668



From daemon Tue May 09 07:20:59 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Tue, 9 May 2000 08:04:36 -0400
Subject: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Gary-
I am not sure if you are saying that your sputter coater is pumping
down poorly or that it is depositing poor quality films. In either case,
it sounds like poor vacuum is the problem. Water vapor adsorbing to the
deposition chamber walls over a period of disuse will pump off of the walls
very slowly. A "wet" chamber can take 10 times as long to pump down to
ultimate vacuum as a "dry" one. If this sounds like it might be causing
the symptoms you are seeing, I would suggest you try one of two things:

1. Place a valve between the sputter chamber and the pump. Valve off the
chamber and leave it under vacuum between uses. As long as it retains any
vacuum this will help when you try to use it again. You don't want to be
pumping on the chamber at the pump's ultimate vacuum for any extended
period of time because that will allow oil vapor from the mechanical pump
to backstream into your sputter chamber and possibly onto your specimen if
present! (I am assuming that you have an oil based pump.) It is also nice
to spare the pump the wear and tear of pumping continuously over periods of
disuse.

OR
2. Several hours or the day before you want to use it, start pumping on the
sputter chamber while admitting a small flow of argon. The argon is an
important part of this procedure. First of all, the argon will prevent the
backstreaming described above. Second, I have been told that the argon
helps to desorb the water from the chamber surfaces. I don't know if that
is an old wives tale or not, but it does seem reasonable. The argon flow
may also help in purging any condensed vapors from the pump's oil. Don't
use too much argon though or you may overheat your pump.

I hope that this addresses the problem you are experiencing.
Matt Ervin
(301)394-0017
U.S. Army Research Laboratory
Adelphi MD




Gary Radice {gradice-at-richmond.edu} on 05/08/2000 03:10:46 PM

To: Microscopy-at-sparc5.microscopy.com
cc:


I have a question about how best to maintain a sputter coater that is used
infrequently.

We are a small college with correspondingly small EM facility. Our SEM and
prep equipment may go for several weeks without being used, then someone
will need it heavily for a class for three or four weeks. Invariably we
find that our sputter coater seems to be the weak link in our plans, since
it rarely works well when we fire it up after long periods of dormancy. I
understand this is common with vacuum equipment: better to use it often
rather than shut it off.

Am I correct that we should we have a plan to regularly pump down the
sputter coater, and if that is good idea, how often should that be? Every
day? Once a week? Once a month?

Or, if we don't need to pump it down regularly, are there other things we
should be doing do it the down time instead of letting it just sit there?

and finally, are some designs better able to handle long periods of disuse?
Do we just have the wrong sputter coater?

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice









From daemon Tue May 09 08:10:16 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 9 May 2000 08:15:00 -0500
Subject: Re:RE: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree. In addition...
Given a choice, I would always use the process gas to purge - using the
"leak
valve" to feed a partial pressure to the system until the vacuum pump heats
up.
Opening the ballast valve will help (lowers ultimate vacuum when open) purge
the
pump, but IMHO, it is better to use dry gas than atmosphere.

Woody White
McDermott Technology

{SNIP}
I suggest that at the end of your spasmodic activities the pump should be
run
with the baffle valve partially open for at least 30 minutes. The baffle
valve
is usually atop the rotary pump. You could also use the sputter coater's
needle
valve, partially open.



From daemon Tue May 09 08:10:16 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Tue, 9 May 2000 08:47:12 -0400
Subject: answers to my sputter coater problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to my query about how best to maintain my
infrequently used sputter coater. Since some have replied off list and
others have asked to see all the responses I thought I would append all of
the responses here, (without direct attribution, since strictly speaking I
don't have all the authors permission to do this....I hope they understand).

Thanks to all who responded. Based on the comments, I think my problem was
accumulation of water in the oil and vacuum surfaces, and dried out
gaskets. Greasing the gaskets and running the pump longer got the pump-down
time from 20 minutes to 3 minutes. Keeping the chamber under vacuum isn't
practical with our coater design, but I can probably solve my problem by
arranging to pump down the chamber once a week, paying attention to keeping
the seals lightly greased, and changing the pump oil on a regular schedule.


******************

} Hi Gary,
}
} How are you? Our sputter coater has sat for over a year at a time without
} use. It had no problems when it was started. However, that is not an
} ideal situation. Vacuum equipment whould be regularly pumped down to
} out-gas the chamber, etc. We have a Denton Desk II Sputter Coater. It is
} by far the best I have used. It is now used regulary because we have a new
} SEM. If I were you I would pump down the chamber on the coater at least
} once a month. Change the oil in the pumps once/year. Check the seal
} between the chamber and the base and the glass and the seal before the
} class use starts. Use fomblin grease or some other non-hydrocarbon vacuum
} grease.
*************

} There really should not be any heroics needed in order to snap your figers
} and have the sputter coater work well. We ship our coaters all over the
} world to trade shows, open up the boxes, take them out, put them on the
} table, and a few minutes later we are coating samples for prospective
} customers. And I think that would probably be the case for most
} commercially made coaters today that are used in the SEM market.
}
} But I will tell you one thing that does happen and that is that the needle
} valve can develop a "set" if it is left tightened down real tight over long
} periods of time.
}
} The when you go to use it again, because of the "set", there are problems
} controlling its action and therefore the bleed rate of air or inert gas. I
} know that conventional wisdom says a vacuum system should be stored "under
} vacuum" but the typical coater is sufficiently leaky, that the vacuum is
} going to disappear shortly anyhow.
}
} So you might want to try storing it no under vacuum, that is, with the need
} valve open, and see if that does not make your problems disappear.

******************

} Hello Dr. Radice,
} Yes, the worst thing you can do to a vacuum system is to not use it. The
} least you should do is keep the bell jar under vacuum when it is not being
} used. Do you vent with Dry Nitrogen or room air? All high voltage leads
} and feed throughs should be kept very clean since they have a tendency to
} collect Carbon and crud. It might not be a bad idea to pre-run your high
} voltage up rather high (higher than you would normally use it), pror to a
} run. This should stabilize things a bit.

***********

} Gary,
} After many years of using sputter coaters I have found that it is best
} that they are kept under vacuum all the time. Many sputter coaters do
} not let you maintain a vacuum when they are off. With this type I have
} found that I have to keep the glass cylinder and the metal target very
} clean and allow plenty of pump down time when first using the unit after
} prolonged shut down. Also any solvent based glues that may be used,
} silver dag or colloidal carbon, must be dry before putting the sample in
} the coater, at least 2 hours after mounting.
**************

} I have a similar use pattern as yours, but I have never had any problems
} with my coater. Sometimes, our coater my sit for a period of months before
} a pump down is required. Back in 1993 I purchased an Emitech, the same
} type that is sold through EMS today, and have had but only one failure.
} That failure was due to foil on a circut board that was not heavy enough to
} handle the current required by the vacuum pump. The foil had melted, but
} was an easy fix for me. Otherwise I have had no failures.
}
} It certainly can help to pump it down once a week, just like it helps
} ink-jet printers to print a test page once a week. Other than keeping
} non-contaminated oil in the pump, a light coating of vacuum grease on the 0
} rings to keep them from drying out and getting attacked by ozone, I have
} not had other maintenance issues. Perhaps your vacuum problems are related
} more to your pump and the need for some maintenance and oil change, perhaps
} your seals need replacing, or perhaps your coater is going through a period
} where it requires higher than normal maintenance. I'm curious, what type
} of problems are you having?
}
} Good luck with your facility. I always enjoy meeting others who are
} running small EM labs.
*******************

} All vacuum systems are better "stored" under vacuum, with the exception of
} any
} systems that cannot vent the rotary pump when power is off. Those systems may
} suck oil from the pump towards the vacuum chamber.
}
} The more common problem for infrequently used systems is moisture in the pump
} oil. Particularly in moist climates and when relatively short pumping
} times are
} employed a good deal of water is absorbed in the pump. When the pump is not
} used for a lengthy period this may cause corrosion and certainly lowers the
} vapour pressure of the contaminated oil and so lower performance results.
}
} I suggest that at the end of your spasmodic activities the pump should be run
} with the baffle valve partially open for at least 30 minutes. The baffle
} valve
} is usually atop the rotary pump. You could also use the sputter coater's
} needle
} valve, partially open. Under these conditions the pump will run hotter and
} throw-out a good deal of oil mist and water vapour. Vent the exhaust into a
} fume hood since oil mist is not just unpleasant. You will find that the pump
} performs much better after a baffle run.
}
} SEM and TEM usually do not need this treatment, but they may, for instance
} if a
} TEM has been used to "dry" film that was not previously dried in another
} system.
*************

} We used to leave the sputter coater sitting for weeks after pumping it down
} and never had any problems. It sounds as if you may have a leak at your
} sealing surface. We always had to be very careful about cleanliness of the
} bell jars surface and the plate it seals on. A small grain of sand or other
} material can fracture the glass so that you have leaks and requires
} repolishing of the glass bell jar.
}
} I hope that this helps you.
***************

} Gary-
} I am not sure if you are saying that your sputter coater is pumping
} down poorly or that it is depositing poor quality films. In either case,
} it sounds like poor vacuum is the problem. Water vapor adsorbing to the
} deposition chamber walls over a period of disuse will pump off of the walls
} very slowly. A "wet" chamber can take 10 times as long to pump down to
} ultimate vacuum as a "dry" one. If this sounds like it might be causing
} the symptoms you are seeing, I would suggest you try one of two things:
}
} 1. Place a valve between the sputter chamber and the pump. Valve off the
} chamber and leave it under vacuum between uses. As long as it retains any
} vacuum this will help when you try to use it again. You don't want to be
} pumping on the chamber at the pump's ultimate vacuum for any extended
} period of time because that will allow oil vapor from the mechanical pump
} to backstream into your sputter chamber and possibly onto your specimen if
} present! (I am assuming that you have an oil based pump.) It is also nice
} to spare the pump the wear and tear of pumping continuously over periods of
} disuse.
}
} OR
} 2. Several hours or the day before you want to use it, start pumping on the
} sputter chamber while admitting a small flow of argon. The argon is an
} important part of this procedure. First of all, the argon will prevent the
} backstreaming described above. Second, I have been told that the argon
} helps to desorb the water from the chamber surfaces. I don't know if that
} is an old wives tale or not, but it does seem reasonable. The argon flow
} may also help in purging any condensed vapors from the pump's oil. Don't
} use too much argon though or you may overheat your pump.
******************

} While I have done no experimental protocol to prove this schedule is
} optimum, during the off season I try to remember to run the sputter
} coaters overnight one night a week. This keeps things in good shape. I
} have read that the slow pumpdown of unused vacuum systems is caused mostly
} by water vapor adsorbed on interior surfaces, and by traces of moisture in
} the pump oil.


Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice




From daemon Tue May 09 10:59:58 2000



From: Edward J. King :      king-at-biology.utah.edu
Date: Tue, 09 May 2000 09:45:53 -0600
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This position is available at the University of Utah. Dr. Erik Jorgensen
is the contact person.

________________________________________

Electron Microscopy Lab Technician

Experience in electron microscopy and a Bachelor's degree in a science
or
related field required. Degree in a biological science, experience in
light
microscopy, and familiarity with microcomputers preferred. Operates
electron microscopes, prepares specimens for microscopy, produces
photographic and digital micrographs, analyzes data and maintains
accurate
work records. Necessary training will be provided. Applicants must
submit a
University of Utah Application for Employment.


Erik M. Jorgensen, Ph.D.
Assistant Professor
Department of Biology
University of Utah
257 South 1400 East
Salt Lake City, UT 84112-0840
PHN: (801) 585-3517
FAX: (801) 581-4668
jorgensen-at-biology.utah.edu



From daemon Tue May 09 10:59:58 2000



From: Edward J. King :      king-at-biology.utah.edu
Date: Tue, 09 May 2000 09:51:15 -0600
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This position is available at the University of Utah. Dr. Erik Jorgensen
is the contact person.

________________________________________

Electron Microscopy Lab Technician

Experience in electron microscopy and a Bachelor's degree in a science
or
related field required. Degree in a biological science, experience in
light
microscopy, and familiarity with microcomputers preferred. Operates
electron microscopes, prepares specimens for microscopy, produces
photographic and digital micrographs, analyzes data and maintains
accurate
work records. Necessary training will be provided. Applicants must
submit a
University of Utah Application for Employment.


Erik M. Jorgensen, Ph.D.
Assistant Professor
Department of Biology
University of Utah
257 South 1400 East
Salt Lake City, UT 84112-0840
PHN: (801) 585-3517
FAX: (801) 581-4668
jorgensen-at-biology.utah.edu



From daemon Tue May 09 11:59:48 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 9 May 2000 11:52:50 -0500
Subject: Re: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,

Yes, we find that sputter coaters do not like being ignored for long
periods. Probably, the specimen chamber and vac lines adsorb moisture
and other gases from the laboratory and it takes the rotary pump
considerably longer to pump down (many hours versus 15-20 minutes).

You should pump the system down at least weekly for at least an hour.
After pumping for about 30 minutes, allow the Argon gas to leak
through the system. This really purges the residual gases. Then, we
find that if you fill the system with Argon, rather than letting it
fill with room air, it will take considerably less time to get into a
usable range next time.

John B.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Tue May 09 12:09:49 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Tue, 9 May 2000 13:04:28 -0400 (EDT)
Subject: Bio-Rad 600

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello all,
We are offering a Bio-Rad MRC 600 confocal microscopy system for
sale. Included are: Krypton-Argon Laser (low hours) Single, Double,Triple
label capability (lines available are 488,568,633 and 514nm) on a Nikon
Optiphot Microscope (10, 20, and 60 X)-included. Accompanying computer
system running COMOS ver 6.03 and SOM 4.56d with two color monitors and
a dye sublimation printer. Focus motor Laser stand and microscope vibration
platform are also included. All interested please call (410) 955-1365 or
write back. Thank You.

Mike Delannoy
JHMI Microscopy Facility



From daemon Tue May 09 13:56:51 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 09 May 00 11:08:55 -0700
Subject: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
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Dear experts,

I know I should be kept out of the lab but I had to do some special resin embedding.
The problem is that I didn't read my own instructions and made up Spurr resin by adding all the ingredients together and then mixing. This means I added the DMAE before mixing the other components and have ended up with brittle blocks of inconsistant hardness.

I never thought I would be asking this but is there any way that I can recover these blocks to allow me to examine what I have embedded?
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Tue May 09 23:57:19 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 9 May 2000 14:46:13 -1000 (HST)
Subject: Film dessicator

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X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs


Hi, all you experts...

In spite of the digital camera on our LEO 912 EFTEM, we have a couple of
users who are going through huge quantities of film. I need to set up an
evacuated film dessicator (separate from the one on our older TEM), but I
find the non-glass, non-clear-plastic vacuum dessicators in the catalogs
at hand to be enormously expensive. Does anyone have a favorite vendor
and model, or a kludge? I remember one at Berkeley that I think was made
out of a pressure cooker hooked to a vacuum pump...

Mahalo!
Tina

80 degrees F, sunny blue skies, everything in bloom, and promise of surf.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Tue May 09 23:57:21 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Tue, 9 May 2000 18:10:54 -0700 (PDT)
Subject: Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for a working used sputter coater for a descent price or
donation for a university lab. Please contact me at bcraft-at-uci.edu if you
have one available.

Thank you,


#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Tue May 09 23:57:22 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 10 May 2000 13:55:28 GMT+1200
Subject: Viruses and attachments

Contents Retrieved from Microscopy Listserver Archives
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While everyone is focussed on the ease with which viruses can be
transmitted as and within attachments, maybe it's a good time to ask
that postings to the list be only as text messages, not as
attachments.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue May 09 23:57:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 10 May 2000 10:46:41 +1000
Subject: RE: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul, I am not writing because of your "Dear experts" address. An expert is a
squirt under pressure, maybe that suited better the person who mis-mixed the
Spurr's.
Long time ago I read a note that brittle blocks sectioned better after soaking
them overnight in ethanol. I've never tried that, but there is a possibility.
Please let us know if that method is any good.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, May 10, 2000 4:09 AM, Paul Webster
[SMTP:pwebster-at-mailhouse.hei.org] wrote:
}
}
} Dear experts,
}
} I know I should be kept out of the lab but I had to do some special resin
} embedding.
} The problem is that I didn't read my own instructions and made up Spurr resin
} by adding all the ingredients together and then mixing. This means I added
} the DMAE before mixing the other components and have ended up with brittle
} blocks of inconsistant hardness.
}
} I never thought I would be asking this but is there any way that I can
recover
} these blocks to allow me to examine what I have embedded?
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} phone:213 273 8026
} fax: 213 413 6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm
}



From daemon Sat May 13 11:48:10 2000



From: =?iso-8859-2?B?TOFi4XIgSuFub3M=?= :      labar-at-mfa.kfki.hu
Date: Fri, 12 May 2000 17:11:12 +0200
Subject: Need energy-filtered SAED ring-patterns

Contents Retrieved from Microscopy Listserver Archives
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Margaret,
Your message struck a chord here, and I would like to extend my
heartfelt sympathy. We've been there too. I have reached the point
where all the suppliers I contact have a 15-year history of preparing
quotations and tenders almost completely in vain. I don't know why
the sales reps. (or I) put up with it. That they do is a triumph of
hope over realistic expectation, and therefore a credit to all of them.
I think there should be a club for stressed out managers of poorly-
funded EM facilities. I would be one of the first to join. perhaps
some day we should organise a world congress if we can find a
venue large enough, thoough whether it would be advisable to have
a trade exhibition is open to question.
Best wishes
Chris

Date sent: Thu, 11 May 2000 14:43:05 -0400
To: Microscopy-at-sparc5.microscopy.com
} From: Margaret Dienelt {brannign-at-asrr.arsusda.gov}


Dear Colleagues,

I want to test a new computer program of mine that processes electron
diffraction ring-patterns from polycrystalline samples. I did test it with
patterns recorded on film. However, I would also like to test it on energy
filtered patterns that were recorded with a CCD (or imaging plate).

Could anyone of you send me such patterns from a single phase material with
random orientation? If you also characterized the same sample (especially if
you proved that the sample is not textured) and you published the results, I
could reference this publication of yours.

Thank you in advance.

Dr. Janos L. Labar
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/phone: (36)(1) 395-92-32
home page: www.mfa.kfki.hu/~labar




From daemon Sat May 13 11:48:13 2000



From: Mark Riggs :      riggsm-at-svg.com
Date: Fri, 12 May 2000 15:01:23 -0400
Subject: cleaving tools small samples

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Your concerns and frustrations expressed are a recurrent thread on this site. It would be nice to be able to sit down with other facility managers and discuss common problems and possible solutions. I think our common concerns are such that they effect both materials and biological facilities. I am thinking of things such as: use guidelines, multi-user vs. service functions, formal courses and informal instruction for new users, equipment maintenance costs, justification of new equipment needs to administrators, advisor committee formats, funding sources, etc.
Perhaps we could arrange such an informal session at the upcoming M&M meeting. If you would be interested in such a session, take a look at the meeting schedule and suggest a time. Then I will ask the organizing committee to designate a room.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


Margaret,
Your message struck a chord here, and I would like to extend my
heartfelt sympathy. We've been there too. I have reached the point
where all the suppliers I contact have a 15-year history of preparing
quotations and tenders almost completely in vain. I don't know why
the sales reps. (or I) put up with it. That they do is a triumph of
hope over realistic expectation, and therefore a credit to all of them.
I think there should be a club for stressed out managers of poorly-
funded EM facilities. I would be one of the first to join. perhaps
some day we should organise a world congress if we can find a
venue large enough, thoough whether it would be advisable to have
a trade exhibition is open to question.
Best wishes
Chris

Date sent: Thu, 11 May 2000 14:43:05 -0400
To: Microscopy-at-sparc5.microscopy.com
} From: Margaret Dienelt {brannign-at-asrr.arsusda.gov}


recall reading of ibm's hunt for a silicon wafer cleaving tool capable of handling samples 5mm in diameter. any luck out there?

mark riggs
svg lithography
wilton, ct 06897
riggsm-at-svg.com



From daemon Sat May 13 11:48:15 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 12 May 2000 13:00:33 -0700 (Pacific Daylight Time)
Subject: Uranyl Formate

Contents Retrieved from Microscopy Listserver Archives
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We are contrasting connective tissue filaments by
negative stain technique using uranyl formate. Our
protocol is to adsorb filaments onto carbon film coated
grids, then to wash in two drops of water and two drops of
uranyl formate, removing each drop using filter paper but
not allowing the grid to dry until after the second drop of
UF. We can not charge the grid surface prior to specimen
adsorption or our specimens will not adsorb.

My question is to do with making uranyl formate. Our
formula is to boil 5ml water, add .0375 g uranyl formate
(our solid is very old...), stir for 20 minutes, then add
10 microlitter 5 M NaOH, stir for 20 minutes, then filter
through a 0.1 micron before use. We are not getting a
consistant staining pattern. Our wish is to see a uniform
coating of stain and we seldom see even single grid squares
evenly coated. We've tried different concentrations of
stain and also different volumes of NaOH. Any suggestions?
Does anyone know the purpose of boiling the water?

Thanks in advance,

Doug
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Sat May 13 11:48:15 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Fri, 12 May 2000 13:46:01 -0600
Subject: TEM: new developments in imaging systems?

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Margaret,

As a former user and current vendor of such systems as you are inquiring
about I can try to provide a bit of information regarding camera
improvements:

There have been a number of improvements, but I am not sure what you are
comparing the latest cameras against. Cameras are now usually cooled and
provide 12 bits per pixel, the number of pixels has gone up a bit (but
not much in general), and cameras read out faster than they used to (up
to 20 fps and more). I think all cameras now use a line transfer
mechanism, which makes shutters obsolete.
On the software side, real-time FFT and real-time shading correction can
be done now due to faster computers without special processing boards,
and there have been other software developments that make using the
cameras and computers easier.
Other changes that affect the usability of cameras is the use of
pneumatics to insert and retract the phosphors, higher frame rates for
live viewing with the camera, etc.

If you have questions, please give me a call, drop me an email, or go to
our web site.

Michael


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Margaret Dienelt [mailto:brannign-at-asrr.arsusda.gov]
Sent: Thursday, May 11, 2000 12:43 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

Year after year I hopefully gather information about digital imaging
systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
money. This year it looks like it might really happen but I have not
kept up with innovations in the field and am wondering the following:

1. Anything new in the last two years -- especially in terms of
cameras? I'm most familiar with the Gatan and AMT systems but their
web sites don't reflect much in the way of changes over a year ago.
2. With more and more microscopists finally getting their systems --
I'd love to get feedback.

Thanks,
Margaret

P.S. Would welcome contacts from vendors.

--
Margaret Dienelt

Plant Pathologist
Electron Microscopy Lab

Floral and Nursery Plants Research Unit
U.S. National Arboretum/Agricultural Research Service/USDA

B. 010A, Rm. 238, BARC-W
10300 Baltimore Avenue
Beltsville MD. 20705 USA

(301) 504-6097
Fax: (301) 504-5096



From daemon Sat May 13 11:48:19 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 12 May 2000 16:29:56 -0700
Subject: Re: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
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Patrick Echlin from Cambridge UK noted in private message that "strong"
agent who may dissolve epoxy is sodium methoxide. Thanks, Patrick.

Sergey.


} Date: Thu, 11 May 2000 16:06:39 -0700
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: Spurr resin problem
} X-Sender: sryazant-at-pop.ben2.ucla.edu
} To: Microscopy-at-sparc5.microscopy.com
} X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Sat May 13 11:48:20 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 12 May 2000 17:59:03 -0700
Subject: RE: Uranyl Formate

Contents Retrieved from Microscopy Listserver Archives
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Dear Doug,

My procedure for uranyl formate is a little bit simpler than yours:
0.05-0.1 g uranyl formate (0.5-1% final) + 10 ml deinozed (cell culture
quality or double distilled in the glass) water in the 15 ml plastic tube.
It dissolved at the same speed (even better) as acetate salt (UA). Usually
I am using rotator to shake slowly the tube with stain. It takes 0.5-1
hour to dissolve salt completely. I do not filter solution yet. The
difference between formate and acetate salts of the uranium is that formate
is light sensitive (UA - too, but less, less sensitive). You have to avoid
direct high intensity light. Usually I wrapped tube in alumina foil and
prepared the samples under diffused light moderate intensity (general
illumination in the lab, no local lights). Staining procedure is exact the
same as for acetate salt. The advantage of formate salt - it generates
smaller granularity (and less contrast than UA), spreaded sometime better
than acetate salt, and pH is higher. The disadvantage of the formate is
that the water solution is not stable: I do prepare fresh solution every
time I have to work with it. This is great disadvantage of the uranyl
formate. I guess, you may store solution in the dark at +4oC for couple of
days, but this is your own risk to experiment with that. As for staining
procedure, I would avoid any washes with just water. As a biochemist I am
under impression that ionic conditions is important to preserve "native"
structure of the sample. Therefore I am using the same buffer as for
sample to wash (usually I do not wash at all). Of coarse for any uranium
salts you have to avoid any phosphates in the buffer. Any Tris, MES, HEPES
buffers may be the good point to start. I don't know exactly how it works,
but it seems to me, that buffer in the wash may help spread satin better
(don't ask me why, I have no idea). If you have problem to dissolve uranyl
formate, you probably have to replace it on the fresh one (it is cheap).
Double-carbon technique may also help (you may call off line for details).
Good luck and sorry for the long message.

Sergey


} Date: Fri, 12 May 2000 13:00:33 -0700 (Pacific Daylight Time)
} From: Douglas Keene {DRK-at-shcc.org}
} Subject: Uranyl Formate
} Sender: drk-at-shcc.org
} To: microscopy-listserver {Microscopy-at-sparc5.microscopy.com}
} Reply-to: DRK-at-shcc.org
} X-Mailer: Simeon for Win32 Version 4.1.3 Build (39)
} Priority: NORMAL
} X-Authentication: none
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Sat May 13 11:48:25 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 12 May 2000 20:44:02 -0700
Subject: RE: facility problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From Debby Sherman:
}
} Your concerns and frustrations expressed are a recurrent thread on this
} site. It would be nice to be able to sit down with other facility managers
} and discuss common problems and possible solutions. I think our common
} concerns are such that they effect both materials and biological
} facilities. I am thinking of things such as: use guidelines, multi-user
} vs. service functions, formal courses and informal instruction for new
} users, equipment maintenance costs, justification of new equipment needs
} to administrators, advisor committee formats, funding sources, etc.
} Perhaps we could arrange such an informal session at the upcoming M&M
} meeting. If you would be interested in such a session, take a look at the
} meeting schedule and suggest a time. Then I will ask the organizing
} committee to designate a room.

} Debby -

It's too late to add to the program now, but I attended a Long Beach 2001
LAC meeting last week, and there's a committee member there who wants to
organize something. It's almost too late to add programming even for that
one! The solution that I suggested is to start an annual breakfast or
lunch for facility managers, taking care to avoid other large scheduled
meetings (which aren't listed in the program summary). It might even be
possible to get more time Sunday afternoon, pre-opening reception. Contact
the LAC chairs for that: Stacie Kirsch for Philly & Zed Mason for Long
Beach.

Caroline Schooley


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Mon May 15 08:19:03 2000



From: Mark Aindow :      maindow-at-ims.uconn.edu
Date: Mon, 15 May 2000 08:28:37 -0400
Subject: Postdoctoral Position

Contents Retrieved from Microscopy Listserver Archives
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University of Connecticut
Institute for Materials Science

Postdoctoral Research Position in Electron Microscopy Studies
of Fatigue Crack Initiation Sites in Ti-6-4 Alloys

The Institute for Materials Science (IMS) at UConn is an
interdisciplinary center with the threefold mission of fostering
education, research and outreach in all areas of the materials
sciences. Applications are invited for a Postdoctoral Position
to study the microcrystallography of fatigue crack initiation
sites in Ti 6-4 alloys. The appointee will be involved in electron
microscopy studies of failed test pieces produced at Pratt and
Whitney in the previous phase of this program. It is envisaged
that this work will involve extensive SEM and TEM studies in
the IMS at UConn with some use of the FIB/TEM/STEM and
OIM facilities in the High Temperature Materials Laboratory at
Oak Ridge National Laboratory. Candidates should hold a PhD
in Materials Science, Physics or a related discipline and must
have extensive hands-on experience in a broad range of electron
microscopy techniques. Experience in the assessment of deformation
substructures would also be beneficial. The appointment is for one
year in the first instance and is available from June 1st. Screening
of the applications will begin immediately and will continue until the
post is filled.

Applications from under-represented groups, including minorities,
women and people with disabilities are encouraged.

Interested candidates should send a curriculum vitae, including
publication list, and the names of at least three referees with
postal addresses, telephone numbers and Email addresses to:
Dr. M. Aindow, Institute for Materials Science, University of
Connecticut, 97 North Eagleville Road, U-3136, Storrs,
CT 06269-3136 USA. Email: maindow-at-ims.uconn.edu
--
*********************************************************

Mark Aindow, Associate Professor,
Department of Metallurgy and Materials Engineering
Institute of Materials Science, U-136
University of Connecticut, Storrs,
CT 06269-3136, USA

Tel: +1 (860) 486-2644
FAX: +1 (860) 486-4745
Email: maindow-at-ims.uconn.edu

**********************************************************



From daemon Mon May 15 08:19:04 2000



From: Suzannah Mayo :      smayo-at-southernmicro.com
Date: Mon, 15 May 2000 09:02:30 -0400
Subject: Particle size analysis, flyash preparation

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

I have a question concerning sample preparation for brightfield and
polarized particle size analysis. My customer is trying to devise an
experiment to analyze fly ash collected on a filter during smokestack
emissions testing. He collects ~1gr. per sample, however, more is
easily possible. The sample is clumped and incongruent and he would
like a simple protocol for proper sample dispersion on a slide.

Thank you,
Suzannah Mayo



From daemon Mon May 15 17:35:35 2000



From: HDMHOS-at-aol.com
Date: Mon, 15 May 2000 08:19:25 -0500
Subject: pp wires

Contents Retrieved from Microscopy Listserver Archives
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Hello there everyone,
I am having hard time cutting gold coated polypropylene (pp) wires. I am
using a diamond knife, I have embedded the samples into epoxy and hardener, I
will be doing TEM. But when I cut, I get good thickness but the slice keep
rolling up. I have been trying for the past three days and do not have any
luck.
I have change the ratio of the epoxy to hardener from10/4 to 10/6 still no
luck.
Can anyone suggest some changes. Is cryogenic an option here.

Thank you all,

Briget Ngampa
28 Cedar street
LOWELL,MA 01852
Tel (978) 970 0433
Fax (978) 937 2297
EMAIL: hdmhos-at-aol.com




From daemon Mon May 15 17:35:37 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 15 May 2000 11:02:36 -0400
Subject: Re: cleaving tools small samples

Contents Retrieved from Microscopy Listserver Archives
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Hi,
You may want to check SELA. They have an evergrowing line of cleavers for
the semiconductor industry. Contact Efrat Raz: efrat-at-sela.com

Caveat: MME has no financial interest in this product

Good hunting
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^


At 03:01 PM 5/12/00 -0400, Mark Riggs wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 15 17:35:39 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 15 May 2000 10:34:11 -0600 (MDT)
Subject: Re: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
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On Thu, 11 May 2000, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Hildy hello,
}
} I could not understand, how polymerized (mean crosslinked) epoxy may be
} dissolved back in PO? You have to break chemical bonds between polymer's
} chains first and than it will become soluble. I believe, there are some
} very strong oxidizing agents should be used in order to break chemical
} bonds in the epoxies.
}
} Sergey.
}
}
}
} } Date: Thu, 11 May 2000 11:28:43 -0600 (MDT)
} } From: HILDEGARD CROWLEY {hcrowley-at-du.edu}
} } Subject: Re: Spurr resin problem
} } X-Sender: hcrowley-at-odin.cair.du.edu
} } To: Paul Webster {pwebster-at-mailhouse.hei.org}
} } Cc: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} }
} } On Tue, 9 May 2000, Paul Webster wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear experts,
} } }
} } } I know I should be kept out of the lab but I had to do some special
} resin embedding.
} } } The problem is that I didn't read my own instructions and made up Spurr
} resin by adding all the ingredients together and then mixing. This means I
} added the DMAE before mixing the other components and have ended up with
} brittle blocks of inconsistant hardness.
} } }
} } } I never thought I would be asking this but is there any way that I can
} recover these blocks to allow me to examine what I have embedded?
} } } Paul Webster, Ph.D
} } } House Ear Institute
} } } 2100 West Third Street
} } } Los Angeles, CA 90057
} } } phone:213 273 8026
} } } fax: 213 413 6739
} } } e-mail: pwebster-at-hei.org
} } } http://www.hei.org/htm/aemi.htm
} } }
} } }
} } }
} } Hi,
} }
} } First try this: Get a beaker of water, heat it to the highest temperature
} } at which your blocks were polymerized, then subtract 5 deg. After temp
} } has been reached, put in one block. Leave it for 15 min. Take it out,
} } trim it, and section it immediately. Too brittle? Repeat the water soak
} } for 15 min. Repeat again. Not much can be accomplished after one hour of
} } soaking, but this may be different in your case. Sometimes in desperation
} } when I wanted 4 micrometer thick sections from difficult material I have
} } soaked blocks overnight with good success.
} }
} } One time I was given immensely valuable blocks which were so bad (kind
} } expression) that they curled my hair. They could not be cut. I had to
} } get the epoxy out and reembed. (The micrographs later ended up in a
} } publication in the Comp. Neurol. Journal)
} }
} } What I did was to rotate the blocks in vials continuously in the REVERSE
} } order in which they were embedded, with much elongated times. That is,
} } first they went into PO and epoxy (same formulation as the bad embedment)
} } 3:1, for hours, the 2:1 for the rest of the day, then 1:1 for overnight,
} } then pure PO for a whole day. All the above were done with numerous
} } changes. Once I was in PO, pure for a day, most of the epoxy had left the
} } tissue. I then remembedded the same way I deembedded. What a pain. The
} } blocks were never wonderful, but they sectioned OK and went for
} } publication. It should work for Spurr's. Don't give up. When
} } deembedding, I left out the accelerator, of course. It helps to have some
} } very good chocolate on hand for this maneuver.
} }
} } Good luck,
} } Hildy
} }
} } Hildegard H. Crowley
} } University of Denver
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}
}
}
Hi,

Yes, it is a mystery to me that one can get old epoxy out of tissue with
PO. However, I have done it at least 3 times with enough success to
collect data. The only thing I can think of is that at least 10% of
monomers never bind due to the low embed temps we use for our TEM work.
Those will surely dissolve out leaving holes. (I have seen this). PO is
the simplest of epoxies and a very strong solvent. That is all I know. I
never do the "REVERSE" embed unless forced to do it, because it is so
difficult dealing with the final cutting and the staining. And then the
"new" sections are unstable and uneven. I would rather clean a bathroom!
Bye,
Hildy



From daemon Mon May 15 17:35:39 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 15 May 2000 10:38:54 -0600 (MDT)
Subject: Re: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
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On Fri, 12 May 2000, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Patrick Echlin from Cambridge UK noted in private message that "strong"
} agent who may dissolve epoxy is sodium methoxide. Thanks, Patrick.
}
} Sergey.
}
}
} } Date: Thu, 11 May 2000 16:06:39 -0700
} } From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} } Subject: Re: Spurr resin problem
} } X-Sender: sryazant-at-pop.ben2.ucla.edu
} } To: Microscopy-at-sparc5.microscopy.com
} } X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hildy hello,
} }
} } I could not understand, how polymerized (mean crosslinked) epoxy may be
} } dissolved back in PO? You have to break chemical bonds between polymer's
} } chains first and than it will become soluble. I believe, there are some
} } very strong oxidizing agents should be used in order to break chemical
} } bonds in the epoxies.
} }
} } Sergey.
} }
} }
} }
} } } Date: Thu, 11 May 2000 11:28:43 -0600 (MDT)
} } } From: HILDEGARD CROWLEY {hcrowley-at-du.edu}
} } } Subject: Re: Spurr resin problem
} } } X-Sender: hcrowley-at-odin.cair.du.edu
} } } To: Paul Webster {pwebster-at-mailhouse.hei.org}
} } } Cc: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } }
} } } On Tue, 9 May 2000, Paul Webster wrote:
} } }
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Dear experts,
} } } }
} } } } I know I should be kept out of the lab but I had to do some special
} } resin embedding.
} } } } The problem is that I didn't read my own instructions and made up Spurr
} } resin by adding all the ingredients together and then mixing. This means I
} } added the DMAE before mixing the other components and have ended up with
} } brittle blocks of inconsistant hardness.
} } } }
} } } } I never thought I would be asking this but is there any way that I can
} } recover these blocks to allow me to examine what I have embedded?
} } } } Paul Webster, Ph.D
} } } } House Ear Institute
} } } } 2100 West Third Street
} } } } Los Angeles, CA 90057
} } } } phone:213 273 8026
} } } } fax: 213 413 6739
} } } } e-mail: pwebster-at-hei.org
} } } } http://www.hei.org/htm/aemi.htm
} } } }
} } } }
} } } }
} } } Hi,
} } }
} } } First try this: Get a beaker of water, heat it to the highest temperature
} } } at which your blocks were polymerized, then subtract 5 deg. After temp
} } } has been reached, put in one block. Leave it for 15 min. Take it out,
} } } trim it, and section it immediately. Too brittle? Repeat the water soak
} } } for 15 min. Repeat again. Not much can be accomplished after one hour of
} } } soaking, but this may be different in your case. Sometimes in desperation
} } } when I wanted 4 micrometer thick sections from difficult material I have
} } } soaked blocks overnight with good success.
} } }
} } } One time I was given immensely valuable blocks which were so bad (kind
} } } expression) that they curled my hair. They could not be cut. I had to
} } } get the epoxy out and reembed. (The micrographs later ended up in a
} } } publication in the Comp. Neurol. Journal)
} } }
} } } What I did was to rotate the blocks in vials continuously in the REVERSE
} } } order in which they were embedded, with much elongated times. That is,
} } } first they went into PO and epoxy (same formulation as the bad embedment)
} } } 3:1, for hours, the 2:1 for the rest of the day, then 1:1 for overnight,
} } } then pure PO for a whole day. All the above were done with numerous
} } } changes. Once I was in PO, pure for a day, most of the epoxy had left the
} } } tissue. I then remembedded the same way I deembedded. What a pain. The
} } } blocks were never wonderful, but they sectioned OK and went for
} } } publication. It should work for Spurr's. Don't give up. When
} } } deembedding, I left out the accelerator, of course. It helps to have some
} } } very good chocolate on hand for this maneuver.
} } }
} } } Good luck,
} } } Hildy
} } }
} } } Hildegard H. Crowley
} } } University of Denver
} } }
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
} }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}
}
}
Hi,

Absolutely sodium methoxide will take the epoxy out of the tissue.
However, in our laboratory it caused so much tissue damage (since epoxies
actually bind with proteins in the tissue and not simply throw a net
through and around the tissue like the acrylics) that after reembedding it
was not useful for collecting data.
Somebody else might have better results than myself with that method, so
we should not discard the idea.

Bye,
Hildy



From daemon Mon May 15 17:35:39 2000



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Mon, 15 May 2000 13:51:14 -0400
Subject: pp wires

Contents Retrieved from Microscopy Listserver Archives
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Briget: Polypropylene has a Tg of about -19C. Whenever I microtome I
always cut at least 15-20 degrees below the Tg. If you are trying to cut
these samples at room temperature my guess is this is your problem. If you
need to embed you can still do this when cutting at cryo temperatures by
trimming as much of the epoxy away as possible. If you leave a very thin
layer of epoxy around your sample you should still get good sections even
though you will get some chatter in the epoxy region. Steve



Hello there everyone,
I am having hard time cutting gold coated polypropylene (pp) wires. I am
using a diamond knife, I have embedded the samples into epoxy and hardener, I
will be doing TEM. But when I cut, I get good thickness but the slice keep
rolling up. I have been trying for the past three days and do not have any
luck.
I have change the ratio of the epoxy to hardener from10/4 to 10/6 still no
luck.
Can anyone suggest some changes. Is cryogenic an option here.

Thank you all,

Briget Ngampa
28 Cedar street
LOWELL,MA 01852
Tel (978) 970 0433
Fax (978) 937 2297
EMAIL: hdmhos-at-aol.com


Stephen McCartney
Research Associate
2108 Hahn Hall
Materials Institute
Virginia Tech
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Mon May 15 17:35:40 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 15 May 2000 11:19:12 -0700
Subject: TEM help selecting prep. equip.

Contents Retrieved from Microscopy Listserver Archives
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Dear List:

A newly appointed researcher here has asked my advice on several pieces of
TEM spec. prep equipment. I turn to you for helpful suggestions.

The research involves serial sectioning biological tissues and many grids.
EM is a minor, but essential component of the project. The lab runs through
dozens of grids and hundreds of pictures, but only 4 - 6 times a year. Big
bursts of activity followed by long periods of analysis and investigations
using other techniques. Of the hundreds of pictures taken, they may only
use a few for data.

The researcher is looking for ideas on what choices are available, how
useful, and approximate costs of the following:

Digital imaging to add to our existing JEOL 1200EX TEM - I have seen other
threads on this topic and would welcome any new ideas. Digital imaging will
save a lot of time and money since they discard so many pictuers, but the
question of image quality is one I am to investigate.

Ultra microtome - We have an older A/O Ultracut (the model before it became
the Reichert Ultracut E) which is OK for the sectioning we do in the
general lab. But she wants a new one for her exclusive use. The question is
whether a new microtome will allow folks in her lab to do serial
sectioning any faster or easier, or by less skilled users, than our current
system.

Staining machine - Anyone have info on staining machines or systems for
lots of TEM grids. I have never had to do so many grids that this was an
issue, so I have never kept up on the offerings. If you know of something
and/or have experience let me know. Again, this is something she would keep
in her lab.

Tissue processing machine - Same as above for me, never did so much at one
time that I ever thought I needed one of these. The samples to be processed
are C. elegans, anything available that could do these unattended? How
about upkeep and volumes of chemicals needed. Also an item to be kept in
her lab.

I will filter and pass on your comments. Anything you might offer will, as
always, be received with appreciation and thanks.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon May 15 17:35:41 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 15 May 2000 16:07:58 -0400
Subject: Re: TEM help selecting prep. equip.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon,
I can speak to the digital camera at least. We have been using a
Gatan BioScan on our microscope and it serves our needs for 95% of the
images that we produce. It is a very good system, when it is working. We
have had more problems with it than I would have expected. We bought the
system early in the production and perhaps they have worked out the bugs by
now.
-------------------------------------.


} Dear List:
}
} A newly appointed researcher here has asked my advice on several pieces of
} TEM spec. prep equipment. I turn to you for helpful suggestions.
}
} The research involves serial sectioning biological tissues and many grids.
} EM is a minor, but essential component of the project. The lab runs through
} dozens of grids and hundreds of pictures, but only 4 - 6 times a year. Big
} bursts of activity followed by long periods of analysis and investigations
} using other techniques. Of the hundreds of pictures taken, they may only
} use a few for data.
}
} The researcher is looking for ideas on what choices are available, how
} useful, and approximate costs of the following:
}
} Digital imaging to add to our existing JEOL 1200EX TEM - I have seen other
} threads on this topic and would welcome any new ideas. Digital imaging will
} save a lot of time and money since they discard so many pictuers, but the
} question of image quality is one I am to investigate.
}
} Ultra microtome - We have an older A/O Ultracut (the model before it became
} the Reichert Ultracut E) which is OK for the sectioning we do in the
} general lab. But she wants a new one for her exclusive use. The question is
} whether a new microtome will allow folks in her lab to do serial
} sectioning any faster or easier, or by less skilled users, than our current
} system.
}
} Staining machine - Anyone have info on staining machines or systems for
} lots of TEM grids. I have never had to do so many grids that this was an
} issue, so I have never kept up on the offerings. If you know of something
} and/or have experience let me know. Again, this is something she would keep
} in her lab.
}
} Tissue processing machine - Same as above for me, never did so much at one
} time that I ever thought I needed one of these. The samples to be processed
} are C. elegans, anything available that could do these unattended? How
} about upkeep and volumes of chemicals needed. Also an item to be kept in
} her lab.
}
} I will filter and pass on your comments. Anything you might offer will, as
} always, be received with appreciation and thanks.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Mon May 15 17:35:43 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Mon, 15 May 2000 17:29:02 -0600 (Hora est‡ndar de MŽxico)
Subject: starch granules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all - a first attempt at unloading a problem onto the listserver!

I am trying to get thin sections of blue-green algae that are loaded with
starch granules. The problem is that every time we do the preps, we end up
with cells that lose some granules, leaving a gaping hole, or of starch
granules that have holes in the centre. I'm using a standard sort of
method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
and EtOH steps are done on ice, the rest at room temp, the resin
infiltration is done on a rotating mixer. We've tweaked the method around
but nothing seems to work. I've tried using LR White as well, but with no
luck. Can anybody out there help!?

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************




From daemon Mon May 15 17:49:17 2000



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Mon, 15 May 2000 17:39:09 -0500
Subject: TEM, Hitachi model Hu 11a

Contents Retrieved from Microscopy Listserver Archives
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"I am Mark Armogida, VP of Engineering at Ted Pella, Inc. in Redding,
California, USA. We have an old (1964) TEM, Hitachi model Hu 11a, that
is a decommissioned instrument which we are trying to give away for
removal/shipping costs. It was operational a year ago and kept under
vacuum since then. We have the instruction manual. This unit consists of
the main body with diffusion pumps, a large electronics console, a large
oil filled resistor and mechanical pumps. The oil was analyzed and does
not contain PCB's. I estimate that this system weighs several thousand
pounds. I will have it removed for salvage in a week, so please contact
me if you are interested in this system."


Mark Armogida
VP, Engineering & Production
Ted Pella, Inc.




From daemon Mon May 15 17:49:18 2000



From: Pamela Neill :      Pamela.Neill-at-alconlabs.com
Date: Mon, 15 May 2000 17:43:33 -0500
Subject: Philips 300 available

Contents Retrieved from Microscopy Listserver Archives
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I am sending this for a colleague. It was a wonderful little scope while I
was there.
} LifeCell Corporation (The Woodlands, TX and Branchburg, NJ) is closing
their facility in The Woodlands.
} LifeCell has available for donation a Philips EM300 TEM. The scope was in
} working order when shut down in December 1999.
} If you are interested please contact:
} Sy Griffey, Ph.D.
} 908-947-1143 or sgriffey-at-lifecell.com
}




From daemon Mon May 15 18:05:43 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 15 May 2000 16:02:58 -0700
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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At 01:38 PM 5/10/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It seems to me that no digital camera system would work on a SEM
in place of a Polaroid or other film-based output device. Since the
recording CRT in a SEM is based on a sequential line scan, one
would need a camera that would capture each line as it is produced.
Most digital backs are single or triple pass units of a single linear
set of sensors. There are other cameras that do snapshot capture
but even these would not work since the whole image is not present
on the record CRT at the time of taking a picture with the digital
camera. The final image is generated sequentially, line by line,
on the record CRT.

If the SEM image is stored in a frame buffer, the buffer can be
converted to RS-170 TV video and frame grabbed. But the
best that this would typically do is 640 lines.

Its an interesting problem and dilemma about being in a situation
where digital camera products simply won't work in place of
film. But since the goal is to obtain a digital file, why not start
with a digital interface? For example, a passive digital capture
system would transfer the record CRT information to computer
and directly result in a nice digital file. Alternatively, for some
systems, an active system can be applied to directly control the
SEM's beam. In doing so, the range of final digital image
resolution is limited only by the attached hardware system.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Mon May 15 19:10:17 2000



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Mon, 15 May 2000 17:02:06 -0700
Subject: TEM for givaway

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am Mark Armogida, VP of Engineering at Ted Pella, Inc. in Redding,
California, USA. We have an old (1964) TEM, Hitachi model Hu 11a, that
is a decommissioned instrument which we are trying to give away for
removal/shipping costs. It was operational a year ago and kept under
vacuum since then. We have the instruction manual. This unit consists of
the main body with diffusion pumps, a large electronics console, a large
oil filled resistor and mechanical pumps. The oil was analyzed and does
not contain PCB's. I estimate that this system weighs several thousand
pounds. I will have it removed for salvage in a week, so please contact
me if you are interested in this system. I can be reached by e-mail or
by phone at 530-241-2200 ext 212 between the hours of 8:00am and 5:00pm
pacific time zone.



From daemon Mon May 15 17:35:38 2000



From: Dean Miller :      miller-at-anl.gov
Date: Mon, 15 May 2000 11:21:23 -0500
Subject: microscopist/materials scientist position at ANL

Contents Retrieved from Microscopy Listserver Archives
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Electron Microscopist / Materials Scientist
Materials Science Division, Argonne National Laboratory

The Materials Science Division at Argonne National Laboratory invites
applications for a Staff Scientist position in Electron Microscopy. The
candidate should have a strong background in the various techniques of
electron microscopy and a very strong interest in application of these
techniques
in materials science. The candidate should have a state-of-the-art
knowledge of either analytical transmission electron microscopy including high
spatial resolution x-ray and electron spectroscopy or the application of
electron holography and Lorentz imaging, with a particular emphasis on
field emission (S)TEM. Candidates with exceptional experience in other areas
of microscopy will also be considered.

Areas of particular research interest include defects and interfaces in
materials, in situ studies of critical phenomena, irradiation and ion
implantation effects, and applications of electron holography and Lorentz
imaging. A familiarity with research in one or more of the following areas is
highly desirable: magnetic and superconducting materials, irradiation
effects, ferroelectrics, nanoscale materials, diamond films, and
non-crystalline materials. The successful candidate will work closely with
Electron
Microscopy Center personnel and other research groups within the Materials
Science Division to develop strong research efforts in one or more of these
areas.

Interested candidates should send a curriculum vitae, a brief statement
of research interests and plans, and the names and contact information of
three references to:
Susan Walker, Employment and Placement
Box MSD-210121
9700 S. Cass Avenue
Argonne, IL 60439
TDD: 630-252-7722

Questions can be addressed to Dr. Dean J. Miller, Materials Science
Division, Argonne National Laboratory, 9700 S. Cass Ave., Argonne, IL 60439,
tel. 630-252-4108 (with voice mail), fax 630-252-7529, or miller-at-anl.gov.

Argonne National Laboratory is a multidisciplinary center of energy
research and related scientific studies and is operated by the University of
Chicago for the U.S. Department of Energy. Argonne National Laboratory is a
federal contractor and complies with all federal contractor rules and
regulations regarding the maintenance and implementation of our Affirmative
Action Program.


---------------------------
Dean J. Miller
Materials Science Division
Argonne National Laboratory
Argonne, IL 60439

630-252-4108 (office)
630-252-7777 (FAX)

miller-at-anl.gov






From daemon Tue May 16 07:36:47 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 16 May 2000 07:50:54 -0700
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Gary Gaugler wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 01:38 PM 5/10/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } I'm curious ... has anyone tried simply installing a digital
} } 4x5 back in place of a Polaroid 4x5 back??? For example, see:
} }
} } http://www.phaseone.com/brochures/powerfx.html
} }
} } cheerios, shAf
}
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.
} Most digital backs are single or triple pass units of a single linear
} set of sensors. There are other cameras that do snapshot capture
} but even these would not work since the whole image is not present
} on the record CRT at the time of taking a picture with the digital
} camera. The final image is generated sequentially, line by line,
} on the record CRT.
}
} If the SEM image is stored in a frame buffer, the buffer can be
} converted to RS-170 TV video and frame grabbed. But the
} best that this would typically do is 640 lines.
}
} Its an interesting problem and dilemma about being in a situation
} where digital camera products simply won't work in place of
} film. But since the goal is to obtain a digital file, why not start
} with a digital interface? For example, a passive digital capture
} system would transfer the record CRT information to computer
} and directly result in a nice digital file. Alternatively, for some
} systems, an active system can be applied to directly control the
} SEM's beam. In doing so, the range of final digital image
} resolution is limited only by the attached hardware system.
}
} gary g.
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Modern surfers use PC boards. You can too at
} http://photoweb.net
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Shaf,
Aside from the problems already mentioned, the back alone (without
computer) is almost as expensive as the smallest SEMs. It also has
about 4 times the resolution of the best recording systems out there
(ETEC) so what you'd get is a lot of empty (information-wise) pixels.
It might be adaptable to TEM, though.

For an SEM an active digital control could be set up to gather 10K x 10K
images, but the stability of the column drivers becomes very important.
Some could handle it fairly well while some would again be useless
because their drivers have no noise immunity due to the fact that their
push-pull mag drivers were designed backwards and all powere supply
noise is passed directly to the scan coils.

Ken Converse
Quality Images
third party SEM service
Delta, PA



From daemon Tue May 16 07:46:49 2000



From: youmay4-at-aol.com ()
Date: Tue, 16 May 2000 07:40:06 -0500
Subject: nutritionist microscopist

Contents Retrieved from Microscopy Listserver Archives
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Email: youmay4-at-aol.com
Name: mil may

Question: where can I find certified nutritionist microscopist in different
parts of the us?

---------------------------------------------------------------------------




From daemon Tue May 16 07:46:49 2000



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Tue, 16 May 2000 07:38:03 -0500
Subject: Starch granules in blue-green algae.

Contents Retrieved from Microscopy Listserver Archives
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Embedding and sectioning material containing a lot of starch is always a
problem. If you do not need to look at the structure of the mature
starch grains, you could try embedding the algae first thing in the
morning before they have had time to produce the starch. Most plants
have a low starch content after being kept in the dark for approx 12
hours.
For instance, active, growing leaves are difficult to section when
collected midday, but early morning collecting makes for easy
sectioning.


Jan Coetzee



Mark West wrote:

} I am trying to get thin sections of blue-green algae that are loaded with
} starch granules. The problem is that every time we do the preps, we end up
} with cells that lose some granules, leaving a gaping hole, or of starch
} granules that have holes in the centre. I'm using a standard sort of
} method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
} dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
} around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
} resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
} and EtOH steps are done on ice, the rest at room temp, the resin
} infiltration is done on a rotating mixer. We've tweaked the method around
} but nothing seems to work. I've tried using LR White as well, but with no
} luck. Can anybody out there help!?
}


--
Prof Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/science/electron/emunit1.htm




From daemon Tue May 16 07:46:49 2000



From: moshe_marc-at-gohip.com ()
Date: Tue, 16 May 2000 07:39:23 -0500
Subject: test the quality of difraction limited microscope

Contents Retrieved from Microscopy Listserver Archives
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Email: moshe_marc-at-gohip.com
Name: moshe marcovitch

Question: Dear sir
In order to test the quality of difraction limited microscope I am looking
for reticles that will enable me to produce difraction patterns of about
0.2 micron source can you advice where can I find such reticles . Who can
produce them for me if they are not available comercially .
Best regards
Moshe Marcovitch

---------------------------------------------------------------------------




From daemon Tue May 16 08:46:40 2000



From: tracy gales :      tl_gales-at-fccc.edu
Date: Tue, 16 May 2000 09:37:17 -0400
Subject: Starch granules

Contents Retrieved from Microscopy Listserver Archives
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Mark,

I've seen references to using potassium ferrocyanide reduced osmium
tetroxide to help preserve glycogen. Karnovsky(1971) Use of
ferrocyanide-reduced OsO4 in EM. In Proc.14th Annu.Meet. Am. Soc. Cell
Biol., p. 146. Abstract 284.

Tracy Gales
Fox Chase Cancer Center
Electron Microscopy Facility
7701 Burholme Ave.
Philadelphia PA 19111
Tel. 215-728-2484
Fax 215-728-2412




From daemon Tue May 16 09:16:56 2000



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 16 May 2000 09:06:16 -0500
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.

What you're saying is there must be a system out there that
digitizes that single stream of line scan intensities, then
processes all that data inside the computer as an image as
opposed to trying to digitize the frame buffer.

- John



From daemon Tue May 16 09:16:59 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Tue, 16 May 2000 10:05:15 -0400
Subject: Re: test the quality of difraction limited microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Moshe,

Try Klarmann Rulings, Inc., they manufacture reticles. If not a stock item they will make one
to your specification.

There URL is http://www.reticles.com

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net


On Tuesday the 16th of May, 2000 at 07:39:23 -0500, moshe_marc-at-gohip.com wrote and posted:

} Email: moshe_marc-at-gohip.com
} Name: moshe marcovitch
}
} Question: Dear sir
} In order to test the quality of difraction limited microscope I am looking
} for reticles that will enable me to produce difraction patterns of about
} 0.2 micron source can you advice where can I find such reticles . Who can
} produce them for me if they are not available comercially .
} Best regards
} Moshe Marcovitch
}
} ---------------------------------------------------------------------------




________________________________________________________
1stUp.com - Free the Web
Get your free Internet access at http://www.1stUp.com



From daemon Tue May 16 09:27:01 2000



From: Vr. Richard Bejsak-Collorado-Mansfeld :      ricardo-at-ans.com.au
Date: Wed, 17 May 2000 00:16:33 +1000
Subject: New website name available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues
there are new websites names available. It will look like: www.diptera.ws
or www.????.ws more information you can find on www.coleoptera.org in
section {software house}
Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Collorado-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).





From daemon Tue May 16 09:27:06 2000



From: micro-at-ldeo.columbia.edu (Dee Breger)
Date: Tue, 16 May 2000 10:33:14 -0500
Subject: SEM facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Collegues,

In response to the current thread on the problems of facility managers, I
say count me in as being highly interested! If a discussion group does get
together at M&M it would be great to see a report posted on this listserver
for those of us who unfortunately can't attend the meeting. If somebody
could take notes and post them, I for one would be very appreciative.

Thanks!
Dee




From daemon Tue May 16 09:27:08 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Monday, May 15, 2000
Subject: Re: starch granules

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mark,
I have been working with cyanobacteria for over 15 years. We found out in the late 80's that the only way to really hold the starch granules together is by using plunge freezing and freeze substitution techniques. I would substitute in acetone + osmium and embed in Spurr's for normal ultrastructure and use ETOH and embed in HM-20 for immuno.
Check out the following references for details of method and contact me for further explanations:


Schneegart, M.A., D. M. Sherman, S. Nayar, and L. A. Sherman (1994). Oscillating Behavior of Carbohydrate Granule Formation and Dinitrogen Fixation in the Cyanobacterium Cyanothese sp. strain ATCC 51142. J. Bacteriology. 176:1586-1597.

Sherman, D. M., T. Troyan, and L. A. Sherman (1994). Localization of Membrane Proteins in the Cyanobacterium Synechococcus sp. PCC7942. Plant Physiol. 106:251-262

Plunge freezing apparatus can be made in a university shop at relatively low cost and works great for many unicellular organisms.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


Hi all - a first attempt at unloading a problem onto the listserver!

I am trying to get thin sections of blue-green algae that are loaded with
starch granules. The problem is that every time we do the preps, we end up
with cells that lose some granules, leaving a gaping hole, or of starch
granules that have holes in the centre. I'm using a standard sort of
method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
and EtOH steps are done on ice, the rest at room temp, the resin
infiltration is done on a rotating mixer. We've tweaked the method around
but nothing seems to work. I've tried using LR White as well, but with no
luck. Can anybody out there help!?

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************







From daemon Tue May 16 09:46:31 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 16 May 2000 09:47:59 -0500 (CDT)
Subject: Re: starch granules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of
{Pine.WNT.4.10.10005151651240.-3843205-100000-at-mwest.ifisiol.unam.mx}
from Mark West {mwest-at-ifcsun1.ifisiol.unam.mx} :
}
Mark,

If you don't really care about seeing the starch, perhaps you could "light
starve" the algae, put them in the dark for some period of time to deplete or
minimize granule size of the starch, without killing them of course. Then
process as usual. We do this with plant samples to avoid the embedding problems
and the resultant holes in the setions that you describe.

Good luck!

Gib

} Hi all - a first attempt at unloading a problem onto the listserver!
}
} I am trying to get thin sections of blue-green algae that are loaded with
} starch granules. The problem is that every time we do the preps, we end up
} with cells that lose some granules, leaving a gaping hole, or of starch
} granules that have holes in the centre. I'm using a standard sort of
} method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
} dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
} around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
} resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
} and EtOH steps are done on ice, the rest at room temp, the resin
} infiltration is done on a rotating mixer. We've tweaked the method around
} but nothing seems to work. I've tried using LR White as well, but with no
} luck. Can anybody out there help!?
}
} Mark
********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Tue May 16 11:05:58 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 16 May 2000 08:39:02 -0700
Subject: Re: SEM facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Me too, though I would like to attend the meeting in Philadelphia.
Elaine
}
}
} Collegues,
}
} In response to the current thread on the problems of facility managers, I
} say count me in as being highly interested! If a discussion group does get
} together at M&M it would be great to see a report posted on this listserver
} for those of us who unfortunately can't attend the meeting. If somebody
} could take notes and post them, I for one would be very appreciative.
}
} Thanks!
} Dee


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Wed May 24 20:27:06 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 23 May 2000 08:19:09 -0500
Subject: Equipment reliability

Contents Retrieved from Microscopy Listserver Archives
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Martyn,

In my experience, two weeks of downtime a year is not bad at all, for any
EM. Especially when you are dealing with many users with varying levels of
expertise and a heavy usage schedule. EM's are maintenance-intensive
instruments (especially TEM's), and even performing preventive maintenance
routines can easily eat up a week a year.

In my opinion, you're doing great.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: "HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
[mailto:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com]
Sent: Tuesday, May 23, 2000 5:02 AM
To: Microscopy-at-sparc5.microscopy.com



A how long is a piece of string ? type question


I would be pleased if anyone could broaden my views on equipment
reliability .
Basically we have a 3yr old FESEM which I consider to be fairly
reliable in that it is on call 24hrs/day , has numerous ( non
dedicated users ) and apart from downtime for filament change and the
odd wear and tear type problems answers our needs .
As we do not have a back up instrument and when we do experience
problems it's always at the worse time certain personnel have the
impression that it is unreliable .

What do other sem users expect in terms of reliability , apart from my
' subjective ' comments is it quantifiable , would approx 2wks /year
downtime including planned maintenance be considered excessive ?

Regards
Martyn Harris
harrism-at-esm-semi.co.uk



From daemon Wed May 24 20:27:07 2000



From: Belinda White :      whiteb-at-EMU.UNP.AC.ZA
Date: Tue, 23 May 2000 16:16:39 +0200
Subject: RMC cryo-ultramicrotome contact numbers

Contents Retrieved from Microscopy Listserver Archives
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Please could someone from RMC contact me off-line with contact telephone and fax. numbers.

Many thanks

Belinda



Belinda White
Centre for Electron Microscopy
University of Natal
Private Bag X01
Scottsville
PIETERMARITZBURG
3209
SOUTH AFRICA

email: whiteb-at-nu.ac.za
tel: +27 ( 0)33 2605157
fax: +27 (0)33 2605776



From daemon Wed May 24 20:27:07 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Tue, 23 May 2000 10:21:00 -0400
Subject: RE: Equipment reliability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just another opinion...

Two weeks a year (avg) seems like a lot of downtime for a FE-SEM to
me. Our Hitachi S-800 SEM, for example, is 13 years old, just
recently had only it's second emitter replacement, and, not counting
building air, water and power problems, has certainly had only about
4 weeks of instrument-related downtime in that 13 year period,
including annual PM services.

I guess it's tough to break an anvil... ;-).

Larry
PS it's a heavily used multi-user instrument...




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed May 24 20:27:09 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Tue, 23 May 2000 10:58:37 -0400 (EDT)
Subject: Re: Rapid Fixation of cells for SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gib,
Have you thought about microwave fixation in the pressence
of aldehyes (para/GA) followed by microwave fixation in osmium.
each takes seconds and fixations are as good or better (for rapid
fixation) than standard fixation. The key is to keep heat off
the sample (use water baths and/or ice bath). The theory is
that the microwave pulsations increase the penetration speed of
the fixatives. Call Ted Pella's tech divison for more info or
protocols.

Mike Delannoy




From daemon Wed May 24 20:27:10 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 23 May 2000 09:17:16 -0700
Subject: Re: Re[2]:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
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Dear Woody,
I had a problem once that really strained the ultimate Z number sensitivity
of my BSE detector. This was a case of a small amount of a marker chemical,
I think Strontium, being fed to growing fish and trying to detect it in a
scale of the fish. I was definintely able to detect the faint, brighter band
on my GW BSE detector (solid state), but not on my Robinson (scinntilator)
on the other SEM. I had to use more beam current for the GW, but it saw the
contrast when the Robinson didn't. More sensitivity does not mean better Z
resolution.
At 01:36 PM 5/22/00 -0500, you wrote:
}
} Hello Gary,
}
} I have never used a sintillator type BSE detector, but the major differences
} are
} two fold.
}
} Typically (though diodes are getting better all the time) the Robinson has a
} better low energy BSE detection effiency. It follows that for the same
} noise
} level electronics, it would exhibit better sensitivity. The dynamic range
} problem will still exist for very different Zs in the field of view. In the
} middle and upper portions of the sensitivity range (low and lower), I would
} not
} expect much difference between them. ...Any Comments from users of both????
}
} I like the 4 quad diodes since I can go differential mode for macro
} topography
} (like fracture surfaces) and show the gross features while suppressing the
} fine
} detail.
}
} The Pt coating can have a profound negative effect on sensitivity if not
} extremely thin. If given a choice, I would always use carbon for the best
} BSE
} sensitivity. ...If not hazardous, I would use Be, BUT!!!! For elements in
} the
} range of Al & Si, I prefer to use carbon and lower beam voltages to minimize
} penetration, especially if they are films or very small features.
}
} I once presented some data illustrating the BSE signal attenuation as a
} function
} of sputtered Au thickness. But that data would be hard to retrieve now.
}
} Woody White
} McDermott Technology

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 24 20:27:10 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 23 May 2000 12:49:29 -0400
Subject: PtC shadowing question

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Dear Listers,
I now know that the size of the gold used in the label was
0.8 nm and the DNA in another exp. will be ssDNA (7249 bases,
circular). We're prepared to try 1.4 nm and 3.0 nm and darkfield
before shadowing. I have copied the repsonses below and will post
the results of our efforts. Once again, I am most grateful for your help.
Rosemary

I don't think the PT shadowing would obscuring the gold labelling. I did
some rotary shadowing of myosin molecules with antibody attached. You
couldn't see the actual Y structure but you could see arrowheads. If you
can see isolated IgG molecules you should be able to pick up the gold
particles. Patty Jansma

Do you mean you want to see a gold particle in a preparation that is
shadowed with Pt after the gold labelling step has take place? If so,
the answer is probably "yes." The Pt gives such a high-contrast
shadow that it may be difficult to pick out the small gold probe
against the contrasty background. Carol Heckman

NO, it should enhance the whole image so that you can more readily see
exactly where the gold is labelling. Cheers,Marilyn Henderson

I would suggest to use dark field imaging of your labeled DNA-protein
complexes in TEM. By using this technique, you do not need to use Pt-
shadowing of your samples. Best regards from Prague. O. Benada

Rosemary, The typical "grain" size using Pt/C is on the order of 0.8 nm.
So the answer to your question would be dependent on the size of the gold
probe you are using. I would think that you would be OK with a gold probe
larger than 3 nm, but this is speculation on my part, I have not actually
done that exact thing with my own two hands. Chuck (Charles Garber)


Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html




From daemon Wed May 24 20:27:11 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Tue, 23 May 2000 15:32:39 +0100
Subject: Sputter Coating

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Hi

Sure it is possible to melt samples by sputter coating but it is usually
when you are using the old fashioned "brute force" systems that use a variac
to adjust the operating voltage.

A big problem with sputter coating is that many of the solutions to one
problem are the reasons for others!

1. To help prevent melting increase the working distance (} 5cms) and cut
down the current (~10mA) - problem this cuts down the coating thickness so I
need to coat for longer!

2. To help prevent melting use a number of short coating periods with a
cooling down period between - problem multi coats build structure on the
specimen and from tests I have conducted the first 10 seconds of the plasma
are the hottest! We actually use the multi coat method to make test
specimens ( 5 x 1 minute coats at 20mA 5cms working distance with 1 minute
between coats).

3. During the early days of SEM sputter coating I would place the sputter
head in a refrigerator for an hour prior to use, part of my method in order
to tray and track down the heat problems. There was considerably less
heating under these circumstances but we were very very careful with
condensation on the then "high voltage" connection. This test led us to
talk very seriously about water cooling the sputter head, a route that was
taken by Baltzers at one stage.

4. My route for a specimen that melts would be to cut down the current,
give a longer coating time and provided you were not working above 5,000X
have a few minutes "cooling time" between no more than three coating
sessions.

Good luck

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com




From daemon Wed May 24 20:27:11 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Tue, 23 May 2000 21:20:39 +0100
Subject: FEG Instrument Reliability

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Hi

I have been involved with clients around the world since FEG systems first
became available. I have found this style of equipment to be very reliable
with if anything less down time than the conventional W hairpin instruments.

I must say that I have seen a considerable difference in the performance of
these instruments with a certain manufacturer's range of FEG microscopes
being far less of a problem in the production of very high quality results
than any of the others.

This said if you are only averaging two weeks per year down time there
should be no one within your organisation who should complain.

As a consultant and ex service engineer I can only suggest that people look
at other instruments within your establishment of equal complexity and
compare the up time performances.

If we can help a phone call consultation costs nothing?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Wed May 24 20:27:13 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Wed, 24 May 2000 09:37:57 +1000
Subject: RE: Equipment reliability

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And another -
same as Larry really, we have a four year-old Hitachi 4500 that has had no
emitter deterioration and probably 5 days total downtime counting
bakeouts. Touch wood. Also in a multiuser facility. But this FESEM series
are proverbially reliable and in general two weeks average per year doesnt
seem excessive, particularly if the FESEM is only three years old, and some
teething problems with any EM column would not be unusual in the first
couple of years. Depends on the context what is acceptable I guess, but if
absolute reliability is a requirement, 24 hr access by "non-dedicated"
users might be the first point to examine?
good luck,
Sally Stowe


} } } Larry Allard {l2a-at-ornl.gov} 05/24/00 12:21am } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Just another opinion...

Two weeks a year (avg) seems like a lot of downtime for a FE-SEM to
me. Our Hitachi S-800 SEM, for example, is 13 years old, just
recently had only it's second emitter replacement, and, not counting
building air, water and power problems, has certainly had only about
4 weeks of instrument-related downtime in that 13 year period,
including annual PM services.

I guess it's tough to break an anvil... ;-).

Larry
PS it's a heavily used multi-user instrument...




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865-574-4981
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From daemon Wed May 24 20:27:13 2000



From: J. Sopp :      jmsopp-at-u.washington.edu
Date: Tue, 23 May 2000 17:37:46 -0700 (PDT)
Subject: gold sample

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hello,

I hope that someone can make a suggestion to me. I am looking for a
sample to use as a standard for low level nanoindentation.

What would be ideal is a gold film of perhaps 1 micron or more on perhaps
polished silicon wafer. This needs to be as smooth as possible, rms
roughness of less than a nanometer. I know that this is quite possible, I
have samples that are just like this but they are too thin (15nm).

does anyone have a suggestion?

thanks,

Jeffrey Sopp




From daemon Wed May 24 20:27:13 2000



From: H. Fong :      hfong-at-u.washington.edu
Date: Tue, 23 May 2000 17:48:56 -0700 (PDT)
Subject: Al peak in EDS spectrum

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We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
problem is that we always get an aluminum peak in every EDS sprectrum
regardless of sample & scan size. Does anyone have any suggestions on why
this is?

Thanks,
Hanson Fong

Univ. of Washington
Materials Science & Engineering Department
Box 352120
Seattle, WA 98195
USA



From daemon Wed May 24 20:27:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 23 May 2000 20:26:44 -0700
Subject: species analysis in real time

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This may be off-topic for this listserver but I'll give it
a shot for those who are into materials science.

Suppose that you have an etching system based
on a small plasma chamber using CF4 and O.
Is there some simple/convenient way to measure
species during the etching process to indicate that some
end point has been reached? i.e., a major etching
area has been etched and the nature of the species
has dramatically or noticeably changed?

I can think of many x-ray methods, but what I am
looking for is basically a sensor of some sort at a
port in the etching chamber system. There is no
SEM beam--and presumedly, no direct or indirect
x-rays.

Any ideas?

gary g.



From daemon Wed May 24 20:27:48 2000



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 24 May 2000 01:07:32 -0400
Subject: Re: Al peak in EDS spectrum

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Hanson,

Are you sure it's Al and not Br? If your accelerating voltage is not high
enough to stimulate the emission of Br K radiation, it can be easy to mistake
the Br L lines for the K lines of Al which are slightly narrower but at the
same peak position. Like the other halogens, Br can initiate corrosion of
metals and end up in the corrosion products. And if this metal is part of
your detector window.....

John Twilley
Art Conservation Scientist

H. Fong wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
} Univ. of Washington
} Materials Science & Engineering Department
} Box 352120
} Seattle, WA 98195
} USA





From daemon Wed May 24 20:27:49 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Wed, 24 May 2000 09:30:35 +0200
Subject: RE:species analysis in real time

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A change in the absorption spectrum of light could to be used as a cut off.
The photon penetration is of the order of the first few monolayers. The
difficulty is using sources and filters of the right frequency range that
is compatible with the etchants and the material you want to detect (when
it becomes exposed).

You would need to find out the optical reflectivity/absorption of the
materials involved. Is this feasible?
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Wed May 24 20:27:49 2000



From: Gunnar Kopstad :      gunnar.kopstad-at-medisin.ntnu.no
Date: Wed, 24 May 2000 09:37:39 +0200
Subject: about: Al peak in EDS spectrum

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Hi Hanson Fong,
A possible explanation is that you have a collimator made of aluminium.
Either the collimator is not properly aligned, or, the carbon paint inside
the collimator has broken. Another explanation can be high energy x-rays
hitting the collimator.

Vennlig Hilsen
dr.ing Gunnar Kopstad
overingeni¿r Avd f Patologi, Rit

tlf. 73 86 86 56, tlf. privat 72 88 79 58
pers.s. 967 75 026



From daemon Wed May 24 20:27:50 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 24 May 2000 06:56:30 -0700
Subject: Re: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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Dr. Gary Gaugler wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This may be off-topic for this listserver but I'll give it
} a shot for those who are into materials science.
}
} Suppose that you have an etching system based
} on a small plasma chamber using CF4 and O.
} Is there some simple/convenient way to measure
} species during the etching process to indicate that some
} end point has been reached? i.e., a major etching
} area has been etched and the nature of the species
} has dramatically or noticeably changed?
}
} I can think of many x-ray methods, but what I am
} looking for is basically a sensor of some sort at a
} port in the etching chamber system. There is no
} SEM beam--and presumedly, no direct or indirect
} x-rays.
}
} Any ideas?
}
} gary g.


Gary,
There are some small, inexpensive mass-specs available that can operate
at pressures up to 25 mT. I believe Ferranti in New Mexico is one that
I've seen. I don't know what pressure your plasma system operates at,
but if it's too high, you'd only need a roughing pump to operate this
system.

When I looked into it a few years ago, it was about $3000 plus a
computer to plug it into.

Ken Converse
owner
Quality Images
Delta, PA



From daemon Wed May 24 20:27:51 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 24 May 2000 07:04:46 -0700
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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H. Fong wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
} Univ. of Washington
} Materials Science & Engineering Department
} Box 352120
} Seattle, WA 98195
} USA


Hanson,
I'm not familiar with the 5200 chamber geometry, but I'm sure there is
aluminum in there. The question is: can the detector see the aluminum
and can the aluminum be excited by either backscattered electrons or
x-rays generated by from the specimen? The detector doesn't care or
know WHERE the x-rays come from, only that they are being generated and
are within line of sight of the detector. Often, moving the detector
closer to the specimen and making sure that the collimator is properly
placed on the detector nose will help narrow the field of view.

Ken Converse
owner
Quality Images
Delta, PA



From daemon Wed May 24 20:27:51 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Wed, 24 May 2000 08:50:41 -0400
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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I would check your electron trap and make sure that it is installed
correctly, your detector window is almost certainly coated with Aluminum to
keep light out, electrons striking the window can produce the effect you
are observing. If this is the case you should also be seeing a large hump
in the high end of your continuum. Good luck and let the list know what
you discover.
Scott


}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
} Univ. of Washington
} Materials Science & Engineering Department
} Box 352120
} Seattle, WA 98195
} USA


------------------- note: new mailing address ------------------------
Scott Wight fax: 301-417-1321
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | email: scott.wight-at-nist.gov
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Wed May 24 20:27:52 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 24 May 2000 09:25:50 -0400
Subject: Re: Sputter Coating

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Ron:

If you use a tride sputter coater, you should not experience any
significant heat build-up during a normal coating process, because
the geometry of the sputter target, employing a central magnet
element to create a shaped magnetic field, directs the electrons in
the plasma away from the sample, thus reducing the "I-square-R"
heating. If you are using an older diode sputter coater, it is a
simple matter to use a pulsed coating process to reduce heating
effects. We used this process many years ago before the triode
sputter coaters were introduced. It turns out that a cycle of 1 sec
ON, 2 sec OFF (or maybe it was 2 ON, 1 OFF) ended up generating an
increase in temperature on an insulating sample only to about 40¡C,
or about body temperature (directly measured using thin-wire
thermocouples). We found we could coat a piece of styrofoam cup with
200 of gold using this process in a diode sputterer, and see no
evidence of melting of the structure.

This is basically the suggestion Steve Chapman makes, to use several
brief coating times. The more regulated, very short heating times
with some cooling time in between seemed to do the trick for us. Try
it, you'll like it... :-).

Larry
PS I think we published this somewhere...if I find it, I'll post.





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Dr. Lawrence F. Allard
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High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed May 24 20:27:53 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 24 May 2000 09:31:50 -0400
Subject: Re: Al peak in EDS spectrum

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How big is the Al peak compared to other peaks? Does it vary according to
the sample composition? Do you get it even if there is no sample in place?
Are you sure you have the sample height correct (the "nominal" height is
often not right - you need to check the X-ray count rate vs. sample
height)? Are you certain it is Al (the position is accurate, the peak
width right)? Do you always use the same voltage, or is the peak there at
different beam voltages? Do you have a thin window or Be detector? Is the
detector working normally in all other respects? Has this spurious
response always been present (i.e. since installation in the '80's) or have
you only recently observed it?

Sorry to ask these questions, but they are relevant.

If there really is an Al peak, it means that within the detector's field of
view is something made primarily of Al which is being irradiated either
with x-rays or electrons. Assuming the working distence (sample height) is
correct, this implies some problem with the collimator, because it's
purpose is to eliminate exactly this type of spurious x-ray signal. You do
have your original collimator and electron trap, do you? Has it been
damaged or moved in some accident with the sample stage?

If the Al signal varies strongly with sample composition (for example, much
weaker with a carbon sample than with a tungsten sample) then it could well
be related to backscattered electrons or secondary x-ray flourescence.

If, on the other hand, the signal varies strongly with operating voltage
(especially if the peak moves) then it probably isn't Al at all, but a
spurious response related either to electrons getting through the window or
to electron noise.

No solutions here (and some of my thoughts are included for completeness as
this is a positing going out for everyone to read), but I hope my musings
help.

Tony Garratt-Reed.



At 05:48 PM 05/23/2000 -0700, you wrote:
} ------------------------------------------------------------------------
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Wed May 24 20:27:53 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 24 May 2000 08:07:08 -0600
Subject: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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Gary,

I think there are a few ways of doing that:

1) You should be able to get some spectral data from the plasma itself.
Hook up a spectrometer and you should be able to see the components in
the spectrum. You may have to excite the plasma with some light.

2) Hook up a mass spectrometer (quadrupole). That should be able to give
you masses of the components.

Don't people do that on a regular basis? You may check the web or
literature for "residual gas analysis" or similar.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Dr. Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, May 23, 2000 9:27 PM
To: MSA listserver
Cc: just_in_case_I_bounce


This may be off-topic for this listserver but I'll give it
a shot for those who are into materials science.

Suppose that you have an etching system based
on a small plasma chamber using CF4 and O.
Is there some simple/convenient way to measure
species during the etching process to indicate that some
end point has been reached? i.e., a major etching
area has been etched and the nature of the species
has dramatically or noticeably changed?

I can think of many x-ray methods, but what I am
looking for is basically a sensor of some sort at a
port in the etching chamber system. There is no
SEM beam--and presumedly, no direct or indirect
x-rays.

Any ideas?

gary g.



From daemon Wed May 24 20:27:53 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 May 2000 07:54:58 -0700
Subject: Re: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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This is the same thing as I am trying to do. In my case, the passivation
will be either sinox or PSG. At present, I remove them using two methods.
One is to do a short soak in BOE, rinse & dry. Then plasma etch with
CF4 at about 80 mTorr. Power and gas flow have dramatic effects on
etch rate. Same for dilution of BOE. The challenge is to nicely get
through the passivation and stop at the uppermost SiO2 dry ox layer.

The physical size of my specimens is small. A 3" diameter cylindrical
chamber would be fine. I use a sputter coater now in etch mode (quartz
chamber of course). It seems to me that there would not be much
of a change from a detection/measuring system after the process
finishes off the passivation and reaches the SiO2. Maybe not true.

There have been several good suggestions on the list so far. One I
will check out right away is the residual gas analyzer. I also may
need some different type of etching unit rather than the coater
operating in etch mode. Since I am interested in FA too, the
specimens are too small to justify the cost of impressive huge
etching units.

gary

At 06:27 AM 5/24/00, you wrote:
} Hello Gary,
} I am also interested in this. We make PLD and I work in the FA group. Being
} this as it may I am new to this field and would wish to find a technique to
} get through the passivation and intrametal layers.
} Thank you for any help.
}
} Sincerely,
} Robb Westby
} Associate Reliability Engineer
} Lattice Semiconductor
}
} "Dr. Gary Gaugler" wrote:
}
} } This may be off-topic for this listserver but I'll give it
} } a shot for those who are into materials science.
} }
} } Suppose that you have an etching system based
} } on a small plasma chamber using CF4 and O.
} } Is there some simple/convenient way to measure
} } species during the etching process to indicate that some
} } end point has been reached? i.e., a major etching
} } area has been etched and the nature of the species
} } has dramatically or noticeably changed?
} }
} } I can think of many x-ray methods, but what I am
} } looking for is basically a sensor of some sort at a
} } port in the etching chamber system. There is no
} } SEM beam--and presumedly, no direct or indirect
} } x-rays.
} }
} } Any ideas?
} }
} } gary g.



From daemon Wed May 24 20:27:54 2000



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Wed, 24 May 2000 12:07:07 -0400
Subject: Chemical Microscopy Workshop, LM

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The weekend workshop on Chemical Microscopy, sponsered by the New York
Microscopical Society, originally sheduled for the weekend of May 20 has
been rescheduled for the weekend of June 17 & 18.

This is an opportunty to learn some of the fundamentals of Chemical
Microscopy from Skip Panenik of Trace Analysis.

The course will be held in West Paterson, NJ.

For further information contact Don O'Leary
Phone (201) 797-8849 Fax (425) 988-1415
E-mail donoleary-at-worldnet.att.net

Visit the NYMS website at www.nyms.org

Don O'Leary
Education Chairman , NYMS



From daemon Wed May 24 20:27:54 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Tuesday, May 23, 2000 9:45 PM
Subject: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Guagler and listers:

I am looking at this same problem for my own Plasma cleaning process. I just
purchased a small, fiber optic, emission spectrometer (USB 2000) from OCEAN
OPTICS to examine the light coming from my cleaning plasma. I just setup the
software yesterday and will be trying it today. If I get some end point
results I will repost to this thread in a few days. There is plasma etch
literature that suggests that end points can be observed in the plasma
emission lines.

Ronald Vane
XEI Scientific
(650) 369-0133

-----Original Message-----
} From: Dr. Gary Gaugler {gary-at-gaugler.com}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
Cc: just_in_case_I_bounce {zaluzec-at-sparc5.microscopy.com}



From daemon Wed May 24 20:27:54 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, May 24, 2000 2:26 AM
Subject: about: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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Al is present everywhere in the SEM. X-rays are produced by emission
(electrons hitting the material) and Fluorescence (X-rays hitting the
material). Collimator fluorescence is a major design problem for EDS system
designers. High Z materials for stopping X-rays fluoresce strongly, and Low
Z materials have no stopping power. Collimators often have high Z material
lined with Aluminum to stop X-rays and then filter out the Fluorescence. If
some of the AL is displaced it can be excited by either stray electrons or
x-rays and cause the Al peak you see.

Ronald Vane
XEI Scientific


-----Original Message-----
} From: Gunnar Kopstad {gunnar.kopstad-at-medisin.ntnu.no}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed May 24 20:27:55 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Wed, 24 May 2000 09:56:49 -0700
Subject: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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H. Fong,

On many scopes I've found this was an artifact of an aluminum sample
holder. Painting the surface of the sample holder with colloidal graphite
or using a carbon planchet usually eliminates the problem.

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: H. Fong [SMTP:hfong-at-u.washington.edu]
Sent: Tuesday, May 23, 2000 5:49 PM
To: microscopy-at-sparc5.microscopy.com




We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
problem is that we always get an aluminum peak in every EDS sprectrum
regardless of sample & scan size. Does anyone have any suggestions on why
this is?

Thanks,
Hanson Fong

Univ. of Washington
Materials Science & Engineering Department
Box 352120
Seattle, WA 98195
USA





From daemon Wed May 24 20:27:55 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 24 May 2000 11:42:44 -0700
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hanson,
I solved that problem by cutting a circle of thin lead (Pb) foil to line the
inside of my Al collimator. Poke a hole in the foil for the hole in the
collimator.
At 05:48 PM 5/23/00 -0700, you wrote:

}
}
}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 24 20:27:56 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Thu, 25 May 2000 08:05:01 +1000
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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Dear Hanson,

Could it possibly be that your gold target has worn through to the Al
support? This has happened in our cryochamber before.

cheers,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Wed May 24 20:27:56 2000



From: Emmanuelle diTomaso :      ditomaso-at-sherman.mgh.harvard.edu
Date: Wed, 24 May 2000 19:13:38 -0400
Subject: Technician position available

Contents Retrieved from Microscopy Listserver Archives
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Edwin Steele Laboratory
at
Massachusetts General Hospital
is actively recruiting a
Lab Technician/Research Assistant
with outstanding background in Histology

The Edwin L. Steele Laboratory (MGH/HMS) is committed to improving
the detection and treatment of cancer through a better understanding
of tumor pathophysiology and the molecular and cellular transport
barriers within tumors.

We are actively recruiting a lab technician/research assistant who
has experience in histology, immunostaining at light and electron
microscopy levels and in situ hybridization.

For more information please see our website: http://steele.mgh.harvard.edu

Please send your resume and 3 letters of recommendation to
Dr. E. di Tomaso via email : ditomaso-at-steele.mgh.harvard.edu
or fax : (617) 726-4172.



From daemon Wed May 24 20:27:58 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Wed, 24 May 2000 16:48:12 -0500
Subject: digital and Koehler

Contents Retrieved from Microscopy Listserver Archives
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Hello Friends,
Does anyone know if one needs to make any adjustments/compromises to Koehler illumination when using a digital camera? We are trying out a few demo's and get circular patterns or edge unevenness at optimum Koehler. If the condenser is defocused things are better...but...isn't the entire purpose of Koehler to get the most out of Abbe's equation that we can?? How much of a loss of resolution can be expected without optimum condenser settings.
My sense is that one should always go with great scope alignment. I am just checking to see if any of us old microscopists can be taught a thing or two regarding digital camera set up.
Thanks,
Linda Fox
lfox1-at-wpo.it.lumc.edu



From daemon Wed May 24 20:27:58 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Wed, 24 May 2000 21:37:02 +0200
Subject: Re: Re[2]:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
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Hi
We have a client on an older Leo S200 with the standard solid state BSD
fitted who has to look at slag off their stainless steel plant.
In this slag there are slight compositional differences which they can only
define should they run their filament on first peak. Strange but true!
At first peak the slightest difference can be seen very easily, at
saturation not a chance.
Now I remember that Steve Chapman did give us an explanation for this the
first time we mentioned it but, alas age catches up on me too and I have
forgotten what it was.

Point is, try this on your system. It works for them. Better resolution on
the BSD at first peak than at saturation.

Cheers
Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com


-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Tuesday, May 23, 2000 6:17 PM
To: White, Woody N
Cc: Microscopy-at-sparc5.microscopy.com


Dear Woody,
I had a problem once that really strained the ultimate Z number sensitivity
of my BSE detector. This was a case of a small amount of a marker chemical,
I think Strontium, being fed to growing fish and trying to detect it in a
scale of the fish. I was definintely able to detect the faint, brighter band
on my GW BSE detector (solid state), but not on my Robinson (scinntilator)
on the other SEM. I had to use more beam current for the GW, but it saw the
contrast when the Robinson didn't. More sensitivity does not mean better Z
resolution.
At 01:36 PM 5/22/00 -0500, you wrote:
}
} Hello Gary,
}
} I have never used a sintillator type BSE detector, but the major
differences
} are
} two fold.
}
} Typically (though diodes are getting better all the time) the Robinson has
a
} better low energy BSE detection effiency. It follows that for the same
} noise
} level electronics, it would exhibit better sensitivity. The dynamic range
} problem will still exist for very different Zs in the field of view. In the
} middle and upper portions of the sensitivity range (low and lower), I would
} not
} expect much difference between them. ...Any Comments from users of both????
}
} I like the 4 quad diodes since I can go differential mode for macro
} topography
} (like fracture surfaces) and show the gross features while suppressing the
} fine
} detail.
}
} The Pt coating can have a profound negative effect on sensitivity if not
} extremely thin. If given a choice, I would always use carbon for the best
} BSE
} sensitivity. ...If not hazardous, I would use Be, BUT!!!! For elements in
} the
} range of Al & Si, I prefer to use carbon and lower beam voltages to
minimize
} penetration, especially if they are films or very small features.
}
} I once presented some data illustrating the BSE signal attenuation as a
} function
} of sputtered Au thickness. But that data would be hard to retrieve now.
}
} Woody White
} McDermott Technology

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu May 25 07:18:01 2000



From: Said Mansour :      Said_Mansour-at-ocli.com
Date: Wed, 24 May 2000 20:28:49 -0500
Subject: open microscopy position

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

Optical Coating Laboratory, Inc. (OCLI) a JDS-Uniphase company is looking to
fill a position in the microscopy area in its Analytical Laboratory . The
candiddate is to have an MS or BS degree in materials science, physics or
related areas with experience in AFM , Light microscopy, FTIR,
interferometry...experience in SEM and other electron microscopy, surface
chemistry analysis is a plus. The assumption of the position is immediate. OCLI
is located in Santa Rosa, California, a leader in thin film products for the
telecommunication industry, counter-feiting applications, photonics and a
variety of other products (please visit OCLI's web site:
http://www.ocli.com/career_opps/index.htm, for more information about the open
position and OCLI and its products.)
If you or any microscopist you know is interested, please send your resume to
Human Resources, Att: Earl Jensen or to me directly by responding to this email
or to my address:
Said A. Mansour
2789 Northpoint Parkway
MS 274-3
Santa Rosa, CA 95407

Thank you




From daemon Thu May 25 07:18:01 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Wed, 24 May 2000 19:22:19 -0700 (PDT)
Subject: Sound Proofing room

Contents Retrieved from Microscopy Listserver Archives
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Our SEM room is a little too noise for high res SEM work, so the service
engineering recommended to dampen the noise. I know there is panels I can
put up on the wall to do this. What is the cheapest solution for doing
this?



#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Thu May 25 07:18:02 2000



From: CraigTbird-at-aol.com
Date: Wed, 24 May 2000 23:09:16 EDT
Subject: RE: AL Peaks in EDS Spectrum

Contents Retrieved from Microscopy Listserver Archives
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Hanson,

I have worked on Noran Systems for 10 years. I have seen this problem several
times and always on a JEOL SEM. Most times it was not the detector. It
usually was a problem with alignment of the beam. The obvious point here is
that detectors do not produce x-rays, they detect them.

Regards,

Craig Theberge



From daemon Thu May 25 07:18:03 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Thu, 25 May 2000 00:53:32 -0500
Subject: Re: digital and Koehler

Contents Retrieved from Microscopy Listserver Archives
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} From: "Linda Fox" {LFOX1-at-wpo.it.luc.edu}

} Hello Friends,
} Does anyone know if one needs to make any adjustments/compromises to
Koehler illumination when using a digital camera? We are trying out a few
demo's and get circular patterns or edge unevenness at optimum Koehler. If
the condenser is defocused things are better...but...isn't the entire
purpose of Koehler to get the most out of Abbe's equation that we can?? How
much of a loss of resolution can be expected without optimum condenser
settings.
} My sense is that one should always go with great scope alignment. I
am just checking to see if any of us old microscopists can be taught a thing
or two regarding digital camera set up.
.} } } } } } } } } } } } } } } } } } .

You are giving up a lot of resolution with a digital camera due to the
pixel spacing so the loss of resolution due to non optimal illumination
have little effect on the image quality.

You can also solve the problem by increasing the distance from
the CCD to the eyepeice so the ragged edge doesn't fall on the
the CCD. This would also reduce the loss of resolution due to
the spacing of the pixels. Of course is aslo decreases the coverage
of the image.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Thu May 25 07:18:03 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 May 2000 23:26:13 -0700
Subject: RE: Equipment reliability

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My Amray 1910 FESEM is only used by me. It is up as long
as I say so (don't shut it down, put it in failsafe mode, etc.).
PM takes about one day and this is done per contract twice
a year. The only real bummer is when all 3 apertures have
gone bad, one-by-one over time. Vent, pull the holder, change out
the apertures and evacuate. This takes me about 45 minutes
to accomplish. Each aperture typically lasts about 2 months.
Since Amray gold flashes them, they cannot be flamed. Guess
that is why they call them "consumables."

The only major down time I experienced was with my 1830 load
lock system. The Balzers 240 turbo was going out (high frequency
oscillation). That took about 3 days to fix for a total pump exchange.
Other than this, both systems are very easy to keep running.

If they were in a mixed user environment, I'd opt for the FESEM
over the LaB6. The FESEM is rather tough to screw up....unless
of course, someone really worked at it.

gary g.



At 04:37 PM 5/23/00, you wrote:
} [snip]
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } A how long is a piece of string ? type question
} }
} }
} } I would be pleased if anyone could broaden my views on equipment
} } reliability .
} } Basically we have a 3yr old FESEM which I consider to be fairly
} } reliable in that it is on call 24hrs/day , has numerous ( non
} } dedicated users ) and apart from downtime for filament change and
} the
} } odd wear and tear type problems answers our needs .
} } As we do not have a back up instrument and when we do experience
} } problems it's always at the worse time certain personnel have the
} } impression that it is unreliable .
} }
} } What do other sem users expect in terms of reliability , apart from
} my
} } ' subjective ' comments is it quantifiable , would approx 2wks
} /year
} } downtime including planned maintenance be considered excessive ?
} }
} } Regards
} } Martyn Harris
} } harrism-at-esm-semi.co.uk
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov



From daemon Thu May 25 07:18:04 2000



From: J.Bruyntjes-at-voeding.tno.nl
Date: Thu, 25 May 2000 08:32:54 +0200
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe



From daemon Thu May 25 07:18:04 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 25 May 2000 08:12:38 +0100 (GMT Daylight Time)
Subject: Re: Sound Proofing room

Contents Retrieved from Microscopy Listserver Archives
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Hi Ben,

Assuming that the noise is being generated from equipment
in the room then curtains will reduce the noise level quite
effectively.

Ron

On Wed, 24 May 2000 19:22:19 -0700 (PDT) Ben Craft
{bcraft-at-uci.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Our SEM room is a little too noise for high res SEM work, so the service
} engineering recommended to dampen the noise. I know there is panels I can
} put up on the wall to do this. What is the cheapest solution for doing
} this?
}
}
}
} #######
} #####\_O -Ben Craft-
} ####/\/}
} #### /"
} ### \
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu May 25 07:18:05 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Thu, 25 May 2000 10:42:20 +0200
Subject: Re: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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Radostin Danev schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Sergey and others,
}
} I want to add my 2 cents as I like the topic.
} Most of this is a result of my experience with our TEM 2Kx2K CCD.
} We are working in bright field - so I cannot comment on dark field
} performance
} Several points:
}
} 1. The CCD is much more convenient - you get your pictures instantly.
}
} 2. CCD is linear and has larger dynamic range than the film.
}
} 3. The bad thing about the CCD is resolution - about 4 times lower than that
} of the film (in our camera the pixel size is 30 microns).

If You would choose a CCD with smaller pixel size You would get a better
resolution.

} So if you want to
} work in minimum dose you will do better with film.

Never, a good CCD is much more sensitive than a film.

} As I know the CCD is not
} performing well in terms of signal to noise at low doses (and if you have to
} work at 4 times higher magnification because of the resolution the things
} become much worse).

see above , a smaller pixel gives a better sensitivity and a better resolution.

}
}
} 4. The CCD has smaller observation area - again loss of information.

use the so called Image mounting, than You get very large images with much more
image information due to the larger dynamic range and better sensitivity.

}
}
} 5. I don't know about the detection efficiency compared to the film - it
} depends on the thickness of the phosphorous and the accelerating voltage. If
} a photon reaches the CCD chip it will be detected ... the problems are in
} the conversion electron-photon.

The currently leading CCD systems reach single electron sensitivity at thin
phosphor screens and good resolution.

} There are two sides - if you make the
} phosphorous thicker you will get higher detection efficiency but the point
} spread also increases so always a compromise is made between detection
} efficiency and resolution. When I say detection efficiency this is not only
} related to the detection of single electrons (as it detects single
} electrons) but more to the actual signal detected on the background of the
} noise. Apart from the shot noise additional noise is added due to the
} scintillator driven detection and thermal noise in the CCD chip.

In good, highly sensitive CCD systems the Poisson noise of the incoming signal
is dominating, not the CCD noise.

}
}
} The CCDs are now very popular in diffraction work because of the dynamic
} range and linearity.
}
} Here is one reference where a nice comparison between 2Kx2K CCD and film has
} been made:
}
} Downing, K., Hendrickson, F., Ultramicroscopy, 75, (1999), 215-233

Thanks for that.

}
}
} Best regards,
}
} Rado
}
} ---------------------------------------------------------------------
} Radostin Danev
} Laboratory of Ultrastructure Research
} National Institute for Physiological Sciences
} Myodaiji-cho, Okazaki 444-8585, JAPAN
} e-mail: rado-at-nips.ac.jp
} ---------------------------------------------------------------------

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Thu May 25 07:18:05 2000



From: Van Osta, Peter [JanBe] :      PVOSTA-at-janbe.jnj.com
Date: Thu, 25 May 2000 10:54:51 +0200
Subject: Cellular translocation, possible artefacts

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I have a question about the study of for instance the translocation of a
fluorescent labeled cellular component from the cytoplasm to the nucleus. At
first it seems that labeling the component you want to study and do a
counterstain for the nucleus would be sufficient to give an idea of the
migration of a componenent from the cytoplasm to the nucleus or not.

By doing the experiment this way however you do not have a clue about the
total cell content, because the cytoplasm is not counterstained with a
background stain to show the cell outlines to give an idea of the actual
cell extent. Without a cytoplasm stain, you have no idea of the actual size
of the cell in which the label for the cellular componenent resides. For an
"absolute" idea of the migration I think a background cellular counterstain
is necessary ?

Also the nuceus is thicker than the cytoplasm, so for a given focuslevel
inside the nucleus there is more light falling in the lens form above and
below than in the cytoplasm, which probably will give a non-linear response
curve for the quantification of the translocation ?

Regards,

Peter Van Osta



From daemon Thu May 25 07:18:05 2000



From: Geoff Williams :      Geoffrey.Lloyd.Williams-at-cmich.edu
Date: Thu, 25 May 2000 08:16:57 -0400
Subject: Re: Sound Proofing room

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A clear case for doing the experiment with a fluorescent probe with
a confocal or multiphoton microscope!
Either way, out-of-focus contributions to intensity are not important.
Your confocal image is also a sample from a well-defined volume of
cell or tissue. Therefore, provided you can regard each
compartment as homogeneously labelled the total compartment
(e.g. cytoplasm, nucleus) volume does not need to be determined.

} From: "Van Osta, Peter [JanBe]" {PVOSTA-at-janbe.jnj.com}
To: Microscopy-at-sparc5.microscopy.com


McMaster-Carr has some of the best selection for sound deadening material and
generally a better price than specialty dealers or other distributors.
Their Web site is very functional http://www.mcmaster.com
Delivery has always been more than prompt and they have more indespensible
items for any microscope lab. No lab should go without one of their catalogs.

Sound control products are in my catalog on page 2777-2779 products ranging
from flat foam to sculptured foam to "sono-tech" foam to acoustical quilts to
acoustical cylinders. Looking through this stuff isn't cheap but of the
products I have seen offered other places the prices here are competitive.

Of course you could always head to a carpet store and dig through their
dumpsters for throw-away remnants and hang them on the walls in the scope
room. Two or three layers might work well enough - not sure about the smell
though. . .

Good luck
Geoff

Ben Craft wrote:

} Our SEM room is a little too noise for high res SEM work, so the service
} engineering recommended to dampen the noise. I know there is panels I can
} put up on the wall to do this. What is the cheapest solution for doing
} this?
}
} #######
} #####\_O -Ben Craft-
} ####/\/}
} #### /"
} ### \

--
Geoff Williams,

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Thu May 25 16:29:50 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Wednesday, May 24, 2000
Subject: Fwd: digital and Koehler

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Linda,
Look for a digital camera that allows you to capture an image of the illumination pattern with your specimen slide removed from the beam path. This "background" image should then be automatically subtracted from your final image. This will eliminate not only uneven illumination but also small light distortions due to dirt on lenses (that you cannot get off by cleaning external surfaces), etc. Using this feature permits good Koehler illumination and very even illumination on your final image file.
As an example, the SPOT RT software has a feature called "Flatscreen". I capture images from each objective after checking for proper Koehler illumination. These are stored and easily called up as needed. However, the microscope alignment should still be rechecked prior to capturing images.
This is a very important feature if you do Nomarski/DIC imaging. You can easily smooth out the very directional illumination pattern by capturing the lighting pattern without the sample and then subtracting it automatically when capturing the sample image.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


Hello Friends,
Does anyone know if one needs to make any adjustments/compromises to Koehler illumination when using a digital camera? We are trying out a few demo's and get circular patterns or edge unevenness at optimum Koehler. If the condenser is defocused things are better...but...isn't the entire purpose of Koehler to get the most out of Abbe's equation that we can?? How much of a loss of resolution can be expected without optimum condenser settings.
My sense is that one should always go with great scope alignment. I am just checking to see if any of us old microscopists can be taught a thing or two regarding digital camera set up.
Thanks,
Linda Fox
lfox1-at-wpo.it.lumc.edu





From daemon Thu May 25 16:29:51 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 25 May 2000 11:02:54 EDT
Subject: Re: digital and Koehler

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In a message dated 5/25/00 10:26:43 AM, sherman-at-btny.purdue.edu writes:

} Look for a digital camera that allows you to capture an image of the
} illumination pattern with your specimen slide removed from the beam path.
} This "background" image should then be automatically subtracted from your
} final image. This will eliminate not only uneven illumination but also
} small light distortions due to dirt on lenses (that you cannot get off
} by cleaning external surfaces), etc. Using this feature permits good Koehler
} illumination and very even illumination on your final image file.

One additional note. Using a background image captured with a log-response
camera (e.g., a Vidicon) does call for subtraction. For a linear response
camera (most CCDs unless you are using some built-in gamma circuitry) you
want to divide by the background (ratio of signal to background). Also, the
problem with this method is that it uses some of your dynamic range, so you
effectively cannot handle as great a range from bright to dark.



From daemon Thu May 25 16:29:53 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 25 May 2000 11:23:15 -0500
Subject: Re: Sound Proofing room

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Ben:Ê we had the same problem in in our SEM/FIB rooms.Ê We got large sheets of
egg-crate foam and glued them to the walls.Ê It gives the place sort of a
"rubber room" appearance, but it works really well.

Ben Craft wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} ToÊ Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Our SEM room is a little too noise for high res SEM work, so the service
} engineering recommended to dampen the noise.Ê I know there is panels I can
} put up on the wall to do this. What is the cheapest solution for doing
} this?
}
} #######
} #####\_OÊÊÊÊÊÊÊÊÊÊÊ -Ben Craft-
} ####/\/}
} #### /"
} ###Ê \

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky HoldfordÊ (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Labs--SEM/FIB/FA
Kilby Center West
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Ê



From daemon Thu May 25 16:29:53 2000



From: David Doe :      ddoe-at-foma.wsc.ma.edu
Date: Thu, 25 May 2000 12:17:54 -0400
Subject: LM- Manual for fluorescence microscope

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I received an AO fluorescence microscope from a kind colleague but need
to find an instruction manual for it. The microscope is an AO model 10
or 20 and it has a vertical fluorescence illuminator (model 2071)
attached. Also, I'm looking for a trinocular head for the microscope to
do photography. Any assistance in locating these would be greatly
appreciated.

David A. Doe
--
Dr. David A. Doe
Biology Department
Westfield State College
Westfield, MA 01086
413/572-5291
fax: 413-562-3613




From daemon Thu May 25 16:29:55 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Thu, 25 May 2000 17:07:24 +0100
Subject: BSE Information

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Hi

Yes Luc is right I did give him an explanation.

The amount of backscatter generated from a specimen depends on the kV, the
probe size and the composition of the material under investigation. If the
level of backscatter is insufficient under "normal" saturation conditions
i.e. the gun is correctly saturated, then by de-saturating the larger source
will result in a larger probe dimension on the specimen; increasing the
probe diameter increases the volume of material involved in the production
of BSE.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Thu May 25 16:29:56 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Thu, 25 May 2000 13:29:51 -0500
Subject: RE: new developments in imaging systems?

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I have been following off and on this discussion on CCD cameras for EM.
Much of this discussion has been concerned with "sensitivity". I am being
naive here, but when we talk about "sensitivity" of CCDs, isn't this the same
thing as the QE of camera/chip? I don't think I have ever seen a QE for em
CCD's for various accelerating voltages/wavelenghts. Does the electron beam
directly hit the silicon photodyodes or it there an interface that converts
the incoming electrons to different (longer?) wavelengts? I know em films are
sensitive to specific acc voltages. Are CCD cameras for em the same. For long
exposures it may not matter, but for short exposures or low level intensity
does the QE of the camera come into play?

} ===== Original Message From Jenichen {Jenichen-at-proscan.de} =====
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77025



From daemon Thu May 25 16:29:57 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Thu, 25 May 2000 12:21:30 -0800
Subject: Looking for operators manual for Varian Leak Detector

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Microscopy Experts,
We have recently inherited a Varian 936-40 Porta-Test leak detector. It
came with out any documentation. Big surprise, I know. I called Varian
and they offered to sell me an operators manual for $185.00. This seems
just a bit excessive. If anyone has one, I would be happy to pay a
Xeroxing and shipping fee.
TIA
Kim DeRuyter
Electron Microscopy Technician
308 Natural Science Facility
P.O. Box 755780
University of Alaska
Fairbanks, AK 99775-5780
907-474-5452
907-474-5163 fax



From daemon Thu May 25 16:29:58 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 26 May 2000 08:34:47 GMT+1200
Subject: RE: AL Peaks in EDS Spectrum

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Hi Craig

}
} I have worked on Noran Systems for 10 years. I have seen this problem several
} times and always on a JEOL SEM. Most times it was not the detector. It
} usually was a problem with alignment of the beam. The obvious point here is
} that detectors do not produce x-rays, they detect them.
}
} Regards,
}
} Craig Theberge
}

Did you ever figure out what sort of alignment problem it was, and
from exactly what the Al X-rays were being produced?

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu May 25 16:29:58 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Thu, 25 May 2000 13:11:54 -0800
Subject: Looking for spare parts for an ISI-40

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Looking for spare parts for an ISI-40 SEM. Particularly, column pieces,
apertures, filament assembly and wehnelt cylinders. Any suggestions would
be welcome.

Kim DeRuyter
Electron Microscopy Technician
Room 308 Natural Sciences Facility
P.O. Box 755780
University of Alaska
Fairbanks, AK 99775-5780
907-474-5452
907-474-5163 fax



From daemon Thu May 25 20:51:31 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Fri, 26 May 2000 02:15:07 +0400
Subject: Re: BSE Information

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Hi friends
I agree Steve, sometimes (I seem it depends on electron gun design and
distance between wenelt and anode, wenelt and filament) the first peak
on saturation curve is more than saturation level even in secondary
emission signal.
Victor Sidorenko, ANTRON Co.Ltd., Moscow, Russia


} ---------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America



From daemon Fri May 26 06:08:08 2000



From: Andrew McNaughton :      andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Fri, 26 May 2000 14:37:12 +1200
Subject: Rapid fixation of small hydrophobic molecules

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Hello

Has anyone knowledge of a method to rapidly fix adherent cell cultures so
as to prevent the loss of small hydrophobic molecules? The problem
involves subsequent diffusion of the antibody marker into the cytoplasm.
Material is examined using fluorescence/confocal microscopy The organelles
of interest in this case are mitochondia but general recommendations would
be most appreciated too.

Regards

Andrew McNaughton

______________________________________________________________________________
Andrew McNaughton
South Campus Electron Microscope Unit
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

e-mail: andrew.mcnaughton-at-stonebow.otago.ac.nz
______________________________________________________________________________n




From daemon Fri May 26 06:08:09 2000



From: Corvos-at-aol.com
Date: Thu, 25 May 2000 23:40:03 EDT
Subject: Service Manuals

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All,

I would like to know if any has started an extensive collection of Microscopy
Service Manuals?

Regards,

Walter Protheroe
E-MAC, Inc.



From daemon Fri May 26 06:08:10 2000



From: Moran Scientific :      kmoran-at-goulburn.net.au
Date: Fri, 26 May 2000 14:06:02 +1000
Subject: Re: BSE Information

Contents Retrieved from Microscopy Listserver Archives
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At 05:07 PM 25-05-2000 +0100, you wrote:
Steve Chapman wrote,

} Hi
}
} Yes Luc is right I did give him an explanation.
}
} The amount of backscatter generated from a specimen depends on the kV, the
} probe size and the composition of the material under investigation. If the
} level of backscatter is insufficient under "normal" saturation conditions
} i.e. the gun is correctly saturated, then by de-saturating the larger source
} will result in a larger probe dimension on the specimen; increasing the
} probe diameter increases the volume of material involved in the production
} of BSE.
}
But how does this lead to an increase in the BSE differentiation (implying
more signal and hence less noise). Surely just defocussing would do the same
thing. Defocussing as such should not effect the BSE coeffecient, unless you
had sub surface charging or some other artefact.

Very interesting!
Ken.

Moran Scientific Pty Ltd
P.O. Box 651
Goulburn NSW 2580 Australia
Tel 02 4844 4234 (International, 61 2 48444234)
Fax 02 4844 4291 (International, 61 2 48444291)
Email {kmoran-at-goulburn.net.au}
Web Page http://www.goulburn.net.au/~kmoran/
"Patience accomplishes its object, while hurry speeds to its ruin. Gulistan
1258"



From daemon Fri May 26 06:08:11 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Fri, 26 May 2000 11:37:46 +0200
Subject: Re: new developments in imaging systems?

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hpadams schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have been following off and on this discussion on CCD cameras for EM.
} Much of this discussion has been concerned with "sensitivity". I am being
} naive here, but when we talk about "sensitivity" of CCDs, isn't this the same
} thing as the QE of camera/chip?

No, sensitivity means how many electrons You need for a digital response from the
CCD.

} I don't think I have ever seen a QE for em
} CCD's for various accelerating voltages/wavelenghts. Does the electron beam
} directly hit the silicon photodyodes or it there an interface that converts
} the incoming electrons to different (longer?) wavelengts?

For TEM investigations the high energy electrons (80 - 400 keV) hit a scintillator
(YAG- or Phosphor-screen). These screen emits visible photons (energy in the
region 2eV) which are detected by the CCD. If we would use the high energy
electrons directly onto the CCD the CCD would be damaged.

} I know em films are
} sensitive to specific acc voltages. Are CCD cameras for em the same.

The response for a phosphor scintillator rises linear with the energy, but reaches
saturation for high energies (higher than 200keV). This response depends also on
the material You use and on the thickness of the screen.

} For long
} exposures it may not matter, but for short exposures or low level intensity
} does the QE of the camera come into play?

If You want to get a good statistics of Your signal a response of one digital
count for one electron would be very good. If the application gives You only a
small amount of electrons to detect (Filter applications, low contrast
applications, biological application and other) the sensitivity (reponse) of the
camera should be higher to avoid long exposure times to overcome problems with
drift an sample damage. So the best cameras optimized for high sensitivity (the
screen is directly coupled with a fibreoptic to a cooled slow-scan CCD with up to
16bit digitization) reach more than one digital count per incident electron to
have a good compromise between sensitivity and good statistics.
Standard system with optical coupling do not reach this sensitivity and can be
used only for applications with high beam density.

}
}
} } ===== Original Message From Jenichen {Jenichen-at-proscan.de} =====
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} }
} } Radostin Danev schrieb:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } } -----------------------------------------------------------------------.
} } }
} } } Dear Sergey and others,
} } }
} } } I want to add my 2 cents as I like the topic.
} } } Most of this is a result of my experience with our TEM 2Kx2K CCD.
} } } We are working in bright field - so I cannot comment on dark field
} } } performance
} } } Several points:
} } }
} } } 1. The CCD is much more convenient - you get your pictures instantly.
} } }
} } } 2. CCD is linear and has larger dynamic range than the film.
} } }
} } } 3. The bad thing about the CCD is resolution - about 4 times lower than
} that
} } } of the film (in our camera the pixel size is 30 microns).
} }
} } If You would choose a CCD with smaller pixel size You would get a better
} } resolution.
} }
} } } So if you want to
} } } work in minimum dose you will do better with film.
} }
} } Never, a good CCD is much more sensitive than a film.
} }
} } } As I know the CCD is not
} } } performing well in terms of signal to noise at low doses (and if you have
} to
} } } work at 4 times higher magnification because of the resolution the things
} } } become much worse).
} }
} } see above , a smaller pixel gives a better sensitivity and a better
} resolution.
} }
} } }
} } }
} } } 4. The CCD has smaller observation area - again loss of information.
} }
} } use the so called Image mounting, than You get very large images with much
} more
} } image information due to the larger dynamic range and better sensitivity.
} }
} } }
} } }
} } } 5. I don't know about the detection efficiency compared to the film - it
} } } depends on the thickness of the phosphorous and the accelerating voltage.
} If
} } } a photon reaches the CCD chip it will be detected ... the problems are in
} } } the conversion electron-photon.
} }
} } The currently leading CCD systems reach single electron sensitivity at thin
} } phosphor screens and good resolution.
} }
} } } There are two sides - if you make the
} } } phosphorous thicker you will get higher detection efficiency but the point
} } } spread also increases so always a compromise is made between detection
} } } efficiency and resolution. When I say detection efficiency this is not only
} } } related to the detection of single electrons (as it detects single
} } } electrons) but more to the actual signal detected on the background of the
} } } noise. Apart from the shot noise additional noise is added due to the
} } } scintillator driven detection and thermal noise in the CCD chip.
} }
} } In good, highly sensitive CCD systems the Poisson noise of the incoming
} signal
} } is dominating, not the CCD noise.
} }
} } }
} } }
} } } The CCDs are now very popular in diffraction work because of the dynamic
} } } range and linearity.
} } }
} } } Here is one reference where a nice comparison between 2Kx2K CCD and film
} has
} } } been made:
} } }
} } } Downing, K., Hendrickson, F., Ultramicroscopy, 75, (1999), 215-233
} }
} } Thanks for that.
} }
} } }
} } }
} } } Best regards,
} } }
} } } Rado
} } }
} } } ---------------------------------------------------------------------
} } } Radostin Danev
} } } Laboratory of Ultrastructure Research
} } } National Institute for Physiological Sciences
} } } Myodaiji-cho, Okazaki 444-8585, JAPAN
} } } e-mail: rado-at-nips.ac.jp
} } } ---------------------------------------------------------------------
} }
} } --
} } Best regards / Mit freundlichen Gruessen
} } Dr. Frank Jenichen
} } Proscan elektronische Systeme GmbH
} } Tel.: +49 8195 999 -511 Fax: -512
} } mailto:Jenichen-at-proscan.de
} } ------------------------------------------------------------
} } More information concerning our products
} } and services can be found on our website
} } http://www.proscan.de
}
} Hank Adams
} Manager
} Integrated Microscopy Core
} Molecular and Cellular Biology
} Baylor College of Medicine
} Houston, Tx 77025

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Wed May 17 21:55:49 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Tue, 16 May 2000 12:11:18 -0700
Subject: Treated coverslips

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I suppose in principle a line-scanner type of CCD could be made to
do this, but the practical difficulties in setting it up would be
enormous - the scan system would have to be synchronously
stepping with the vertical progression of the SEM scan, and the
alignment and line geometry of the system would also have to be
exactly right. Not at all easy to achieve. Also, it is fairly well
understood that the resolution of the record screen underrepresents
the resolution of the raw signal fed to it (partly to ensure that lines
are not visible in the image).
Therefore this just seems to be the wrong approach. There are
plenty of low-cost (~10% of the cost of this camera) image
grabbers for SEM that digitise the stream of analogue data fed to
the record tube.

It would be more interesting to consider whether this type of
camera can contribute to high quality TEM imaging. I am not clear
what is limiting the resolution of current TEM digital cameras -
could a high-resolution CCD camera approach the resolution of
TEM film more closely than the current generation of these, or is
the phosphor/YAG not good enough to make it worthwhile.

Chris

Date sent: Tue, 16 May 2000 09:06:16 -0500
To: {Microscopy-at-sparc5.microscopy.com}
} From: John Foust {jfoust-at-threedee.com}


Dear List Members,

Does anyone know of a source for glass coverslips which have been treated
with something to optimize cell growth? A colleague of mine is looking for
some, particularly round ones.

Thanks.


Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu



From daemon Wed May 17 21:56:10 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 16 May 2000 13:05:15 -0700 (PDT)
Subject: Re: TEM help selecting prep. equip.

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On Mon, 15 May 2000 11:19:12 -0700, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Dear List:
}
} A newly appointed researcher here has asked my advice on several pieces
of
} TEM spec. prep equipment. I turn to you for helpful suggestions.
}
} The research involves serial sectioning biological tissues and many
grids.
} EM is a minor, but essential component of the project. The lab runs
through
} dozens of grids and hundreds of pictures, but only 4 - 6 times a year.
Big
} bursts of activity followed by long periods of analysis and
investigations
} using other techniques. Of the hundreds of pictures taken, they may only
} use a few for data.
}
} The researcher is looking for ideas on what choices are available, how
} useful, and approximate costs of the following:
}
} Digital imaging to add to our existing JEOL 1200EX TEM - I have seen
other
} threads on this topic and would welcome any new ideas. Digital imaging
will
} save a lot of time and money since they discard so many pictuers, but the
} question of image quality is one I am to investigate.
}
} Ultra microtome - We have an older A/O Ultracut (the model before it
became
} the Reichert Ultracut E) which is OK for the sectioning we do in the
} general lab. But she wants a new one for her exclusive use. The question
is
} whether a new microtome will allow folks in her lab to do serial
} sectioning any faster or easier, or by less skilled users, than our
current
} system.
}
} Staining machine - Anyone have info on staining machines or systems for
} lots of TEM grids. I have never had to do so many grids that this was an
} issue, so I have never kept up on the offerings. If you know of something
} and/or have experience let me know. Again, this is something she would
keep
} in her lab.
}
} Tissue processing machine - Same as above for me, never did so much at
one
} time that I ever thought I needed one of these. The samples to be
processed
} are C. elegans, anything available that could do these unattended? How
} about upkeep and volumes of chemicals needed. Also an item to be kept in
} her lab.
}
} I will filter and pass on your comments. Anything you might offer will,
as
} always, be received with appreciation and thanks.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
Jon:

A few comments about the equipment. I have done literally thousands of
serial sections, so I think I can offer some first hand comments.

First: My microtome of choice for serial sectioning has been the old
Reichert OMU-3--the predecessor of the Ultracut E. I have to admit that
part of the reason was not wanting to take the time for a learning curve.
The other reason was that I found the illumination system on the old OMU-3
to be outstanding (I did repetitive serial thins and thicks over several
hundred microns to about a millimeter).

Second:Staining machine--I use/have used the LKB/Leice Ultrastainer. The
original LKB unit was a marvel--we did over 300 grids at one point, losing
only one (stuck the forceps through the formvar!!!), and no precipitates.
Can't say the same for the Leice unit, although it should be pretty much the
same. The stains are the biggest variable, as is a really rigorous cleaning
regime. Check it out.

Third: Processor--I use/love/hate the Lynx unit (currently available
through EM Sciences). I had an early unit (from Australia)--no problems
over 3 or 4 years. Have a Leica unit here--it took 4 or 5 years to get it
to work consistently, but now seems ok, and it gets substantial use in
bursts. Check out the RMC/Ventana unit--it is the progeny of the old LKB
unit, and is probably a worthy competitor. (I just can't get one for
demo--they've been promising for nearly 4 years, but I think they gave up
after last year.) Maintenance on both is fairly routine, and consumables
are not prohibitive. Both use very small volumes, and can be used osmium
through pure epoxy (if your protocol is limited to 20 or so steps).
Personally wouldn't be without one.

Hope this helps.

Roger C Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals

Disclaimer: I have no financial interest in any of the products or
suppliers. Just a long time user.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Wed May 17 21:56:17 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 16 May 2000 16:31:45 -0400 (EDT)
Subject: Re: Treated coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Do the cells need some sort of support matrix (e.g., collagen) or are they
just not fond of glass? We do our own treating....collagen, poly-lysine,
there is a mussel protein (not a spelling error - I think it is the byssal
thread stuff from Mytilus sp.) that is sticky (CellTak?)...you can also
dissolve PS culture dishes in solvent and coat glass coverslips with the
resulting goo.

Tamara Howard
CSHL



On Tue, 16 May 2000, Schibler, Matthew wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear List Members,
}
} Does anyone know of a source for glass coverslips which have been treated
} with something to optimize cell growth? A colleague of mine is looking for
} some, particularly round ones.
}
} Thanks.
}
}
} Matthew J. Schibler Ph.D.
} UCLA Brain Research Institute
} 1524A Gonda (Goldschmied) Center
} for Neuroscience and Genetics
} Los Angeles, CA 90095-1761
}
} (310) 825-9783
} FAX (310) 206-5855
} E-mail: mschibler-at-mednet.ucla.edu
}
}
}



From daemon Wed May 17 21:56:17 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Tue, 16 May 2000 16:53:21 -0400
Subject: RE: SEM facility managers

Contents Retrieved from Microscopy Listserver Archives
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I just got off the phone with Stacie Kirsch, and discovered that I was the
3rd caller today asking about 'something in Philaldelphia'! Debby Sherman
was first and is to talk to the Meeting Arrangements person, or some such,
but I (and probably some accompanying colleagues) would be happy to
participate if something informal were organized for the Sunday afternoon.
Our Group at this Canadian federal lab:

- has 8 different beam instruments in 7 distinct 'labs' (and formally
accesses another 2 at an on-site private sector service provider),
- is manned by 18 scientists and technologists, the majority of which
are permanent,
- does a ~50-100 project/yr mix of contract work and core research
projects for and with internal programs and external clients (academics,
industrial, other federal),
- has a significant number (too many!) of internal and external
operators, and
- has a reasonable operating budget (but currently no capital), so
- we have likely encountered some variation of almost every
conceivable problem (and solved only a fraction).

If something comes to pass, now or next year, I would gladly share
experiences with others, especially as this is an aspect of delivering
science that is commonly overlooked. A couple of thoughts:

- this could easily turn into a 'gripe-athon'. Someone should be
prepared to lead it and direct it towards problem-solving if this occurs.
- Ron Anderson and I have made sporadic attempts over the years to
lead something called " Factors Influencing the Establishment of a New TEM
Facility" at numerous workshops, often with great success. This also
touched upon many of the real-world issues of an EM lab, though from the
slightly more positive viewpoint of actually some hard cash in hand.
-

Tom Malis

Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca

----------
From: Elaine Humphrey [SMTP:ech-at-unixg.ubc.ca]
Sent: May 16, 2000 11:39 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Re: SEM facility managers


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Me too, though I would like to attend the meeting in Philadelphia.
Elaine
}
}
} Collegues,
}
} In response to the current thread on the problems of facility
managers, I
} say count me in as being highly interested! If a discussion group
does get
} together at M&M it would be great to see a report posted on this
listserver
} for those of us who unfortunately can't attend the meeting. If
somebody
} could take notes and post them, I for one would be very
appreciative.
}
} Thanks!
} Dee


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca





From daemon Wed May 17 21:56:20 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 16 May 2000 16:23:50 -0500
Subject: Re: Treated coverslips

Contents Retrieved from Microscopy Listserver Archives
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Guess it depends on what you mean by optimize. The intestinal cell
lines i work with prefer bare glass (i wash in acetone, then ethanol,
then lots of dH2O, then boil in dH2O, then place each one on a piece
of filter paper so they aren't overlapping and then autoclave - a
pain in the neck but I think it really matters). i seed the cells in
serum containing medium without any other pre-treatement

I have coated with collagen, fibronectin, etc by placing coverslips
in 24 well trays and adding the matrix material. this makes no
difference or reduces differentiation of my cells.

many labs report cell lines that differentiate better on permeable
filters so nutrients have access to the basolateral membrane.
}
} Dear List Members,
}
} Does anyone know of a source for glass coverslips which have been treated
} with something to optimize cell growth? A colleague of mine is looking for
} some, particularly round ones.
}
} Thanks.
}
}
} Matthew J. Schibler Ph.D.
} UCLA Brain Research Institute
} 1524A Gonda (Goldschmied) Center
} for Neuroscience and Genetics
} Los Angeles, CA 90095-1761
}
} (310) 825-9783
} FAX (310) 206-5855
} E-mail: mschibler-at-mednet.ucla.edu

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed May 17 21:56:23 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Tue, 16 May 2000 15:10:56 -0700
Subject: Facility Managers

Contents Retrieved from Microscopy Listserver Archives
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I very much agree with others in this issue. Unfortunately, I won't be able
to attend this year's meeting, but I will love to find out what others had
to say about managing a multi-user EM facility. Please post the highlights
of that meeting if it takes place in Philadelphia.

Thanks in advance,

Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Wed May 17 21:56:26 2000



From: N. Hayman :      nickh-at-u.washington.edu
Date: Tue, 16 May 2000 15:39:22 -0700 (PDT)
Subject: mounting clay-rich rocks - soils for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
I believe to be on track with mounting clay rich rocks (that
disagregate readily in perturbed water (i.e. shaken or sonicated), but not
in ethylene glycol or ethanol (at rest). My goal is to mount the samples
(whole, not seperates) for analysis in TEM. I have been soaking them in
LR-White at 60C, where fixation occurs without accelerator in less than 24
hours. I am planning to (but have not yet) microtome the samples, or
potentially ion-mill.
Thoughts for the future are to use a set of glassware with a
Millipore filter and vacuum to pull ethanol(cleanser and dilator),
ethylene glycol(for swelling clay component), and an LR White "chaser" to
view "saturated" textures, as opposed to compacted.
My question is this: Can anyone point out pitfalls with this
approach that I am not seeing, again I haven't tried the whole thing yet,
but am in a position to start prepping the samples. In particular, is
microtoming preferred to ion-milling for weak, soft samples that rely on
epoxy for reinforcement? Does LR-white readily pull through a sample given
a weak vacuum and a porous plate (its viscous, but not as viscous as
water, for example)? Does swelling the clays with ethylene glycol and the
like, and then directly mounting, introduce volatiles into the column
under 120-200 kV? Any recommendations are welcome, the literature helps a
bit, but is usually sketchy about these fine details of preparation
procedure.
thanks in advance,
N

_____________________________
Nicholas W. Hayman \
Dept. of Geological Sci., U.W.\ http://www.geology.washington.edu/~hayman
Box 351310, Seattle WA 98195 \_________________________________________




From daemon Wed May 17 21:56:29 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 16 May 2000 15:48:55 -0700
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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Dear John,
That is exactly what passive image capture systems like Quartz PCI do.
At 09:06 AM 5/16/00 -0500, you wrote:

} At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} } It seems to me that no digital camera system would work on a SEM
} } in place of a Polaroid or other film-based output device. Since the
} } recording CRT in a SEM is based on a sequential line scan, one
} } would need a camera that would capture each line as it is produced.
}
} What you're saying is there must be a system out there that
} digitizes that single stream of line scan intensities, then
} processes all that data inside the computer as an image as
} opposed to trying to digitize the frame buffer.
}
} - John
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 17 21:56:31 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 16 May 2000 16:49:51 -0700
Subject: Cryostat manufacturers

Contents Retrieved from Microscopy Listserver Archives
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Greetings all

some of my users think it is tome to retire our ancient but still
functional cryostat. Could anyone who has bought one in the recent past
give me some info about who is making them these days as well as any pros
and cons of these new-fangled models.

Thanks in advance

Steve Barlow




From daemon Wed May 17 21:56:32 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Tue, 16 May 2000 18:57:56 -0500
Subject: Re: (Fwd) Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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} From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
}
}
} I suppose in principle a line-scanner type of CCD could be made to
} do this, but the practical difficulties in setting it up would be
} enormous - the scan system would have to be synchronously
} stepping with the vertical progression of the SEM scan, and the
} alignment and line geometry of the system would also have to be
} exactly right. Not at all easy to achieve. Also, it is fairly well
} understood that the resolution of the record screen underrepresents
} the resolution of the raw signal fed to it (partly to ensure that lines
} are not visible in the image).
} Therefore this just seems to be the wrong approach. There are
} plenty of low-cost (~10% of the cost of this camera) image
} grabbers for SEM that digitise the stream of analogue data fed to
} the record tube.
}
} It would be more interesting to consider whether this type of
} camera can contribute to high quality TEM imaging. I am not clear
} what is limiting the resolution of current TEM digital cameras -
} could a high-resolution CCD camera approach the resolution of
} TEM film more closely than the current generation of these, or is
} the phosphor/YAG not good enough to make it worthwhile.
}

If you control the scan you only need a single light sensitive
element. You step the beam digitize the light, step the beam,
wait for the last spot to go out and repeat. You don't need
a camera. You do need a very fast responding system for
changing electron beams to light.

This system has the resolution of the scan beam. It would not
be particualy fast but you could increase the number of bits
resolution to as large a number as you wanted.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed May 17 21:56:34 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 16 May 2000 18:25:20 -0700
Subject: Re: (Fwd) Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:08 AM 5/16/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


This is true except that the record CRT has a fixed resolution.
These are typically 2000 horizontal lines. Different scan
rates change the pixel dwell time. But the final image is still
not much more than 2000 lines. This is fine for a Polaroid
print. But if using real film, it is less than ideal or optimum.
I find that film has much more resolution than a Polaroid print.
A good alternative is the Polaroid PN (positive/negative).
But one would still have to scan the negative to get a digital
file. This is another topic, all together.


} It would be more interesting to consider whether this type of
} camera can contribute to high quality TEM imaging. I am not clear
} what is limiting the resolution of current TEM digital cameras -
} could a high-resolution CCD camera approach the resolution of
} TEM film more closely than the current generation of these, or is
} the phosphor/YAG not good enough to make it worthwhile.

Not having TEM experience, I cannot comment on this type
of application. But I am experienced with SEM usage. It would
seem to me at first blush that TEM images are continuous whereas
the SEM images are discrete. This would mean that CCD imaging
devices would work for TEM applications but not for SEM. The
common denominator remains the Polaroid and silver emulsion
film.

gg


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Wed May 17 21:56:44 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Wed, 17 May 2000 00:36:22 -0600
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, there is.

It's called a "passive" or "listening" digital image acquisition system,
such as our ADDA II or similar devices from other manufacturers.
Benefits: fairly easy to set up and use. Disadvantages (as opposed to an
"active" or "talking" system): You're still limited to what the
microscope can provide in terms of resolution, dwell time, etc.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John Foust [mailto:jfoust-at-threedee.com]
Sent: Tuesday, May 16, 2000 8:06 AM
To: Microscopy-at-sparc5.microscopy.com


At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.

What you're saying is there must be a system out there that
digitizes that single stream of line scan intensities, then
processes all that data inside the computer as an image as
opposed to trying to digitize the frame buffer.

- John



From daemon Wed May 17 21:57:12 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 17 May 2000 08:39:29 -0500
Subject: Facility manager's meeting

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Thanks to all who indicated an interest in meeting to discuss some common problems associated with managing microscopy facilities at the M&M meeting. I am in the process of arranging this and will send details once we are a bit further along.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Wed May 17 21:57:16 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 17 May 2000 08:49:21 -0500
Subject: Re: SEM: digital 4x5 backs

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Like Gary and Ken have pointed out, there are several reasons why 4x5 backs
won't work. Even if they did, there is another issue. A 4x5 back would
incorporate the photo CRT with its phosphor into the loop. That means the
electronic signal from the PMT or BSE detector has to be converted back
into an analog brightness via the photo CRT, then the digital 4x5 back
would be used to digitize the signal, and that in a most unwieldly manner.

As a rule, if you don't have to convert signals back and forth or pass them
through extra stages of processing, don't do it. I understand photo CRTs
are quite good, but there are extra focus, noise, and calibration factors
involved passing the signal through the CRT. It is far better to take the
signal straight over to digital using a good, single channel A/D converter
for the video (plus one for X and Y position rather than using the millions
of A/D converters in a CCD. Makes for a lot cheaper system, too.

Warren S.

At 04:02 PM 5/15/2000 -0700, Gary Gaugler wrote:
} At 01:38 PM 5/10/00, you wrote:
} }
} } I'm curious ... has anyone tried simply installing a digital
} } 4x5 back in place of a Polaroid 4x5 back??? For example, see:
} }
} } http://www.phaseone.com/brochures/powerfx.html
} }
} } cheerios, shAf
}
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.
} Most digital backs are single or triple pass units of a single linear
} set of sensors. There are other cameras that do snapshot capture
} but even these would not work since the whole image is not present
} on the record CRT at the time of taking a picture with the digital
} camera. The final image is generated sequentially, line by line,
} on the record CRT.
}
} If the SEM image is stored in a frame buffer, the buffer can be
} converted to RS-170 TV video and frame grabbed. But the
} best that this would typically do is 640 lines.
}
} Its an interesting problem and dilemma about being in a situation
} where digital camera products simply won't work in place of
} film. But since the goal is to obtain a digital file, why not start
} with a digital interface? For example, a passive digital capture
} system would transfer the record CRT information to computer
} and directly result in a nice digital file. Alternatively, for some
} systems, an active system can be applied to directly control the
} SEM's beam. In doing so, the range of final digital image
} resolution is limited only by the attached hardware system.
}
} gary g.



From daemon Wed May 17 21:57:19 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 17 May 2000 07:07:12 -0700
Subject: Fwd: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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} Date: Tue, 16 May 2000 14:27:27 -0700
} To: John Foust {jfoust-at-threedee.com}
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
} Subject: Re: SEM: digital 4x5 backs
}
} No. What I was saying was that there is not a digital
} camera system out there that will capture the line-by-line
} recording CRT output--as far as I see it at present.
}
} To get a digital file from the SEM, the method needs to
} be digital but either passively attached to the record CRT
} or actively connected as a replacement for the SEM's
} internal scan generator.
}
} gary
}
}
}
} At 07:06 AM 5/16/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed May 17 21:57:21 2000



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Wed, 17 May 2000 09:37:11 -0500
Subject: TEM Job Announcement

Contents Retrieved from Microscopy Listserver Archives
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ELECTRON MICROSCOPE TECHNICIAN
Naval Research Laboratory

Announcement Number 74-0448-00
http://amp.nrl.navy.mil/code1800/74-0448.htm
Job Title: Physical Scientist, NP-1301-II, $22,563* to $49,794*
(*Includes locality pay)

Description:
The Marine Geosciences Division, Naval Research Laboratory (NRL),
Stennis Space Center, MS, seeks an electron microscope technician to
perform technical duties in support of the Marine Geosciences Electron
Microscopy Center.

The center has a state-of-the-art analytical scanning JEOL JEM-3010 TEM
equipped with a liquid/gas environmental cell, EDXS, and GIF energy
filter. The center is also equipped with a Hitachi H-600 TEM and soon
an environmental SEM. The technician will support ongoing research by
preparing specimens, analyzing samples, and assisting in general
laboratory operations. Ê

Required qualifications include a degree in physical science,
engineering, or mathematics that included 24 semester hours in physical
science and/or related engineering science such as mechanics, dynamics,
properties of materials, and electronics. The candidate must also have
one year of specialized experience equivalent to the Career Level I
(GS-Equivalent 1-4).

All candidates will be rated on the following factors: 1) Knowledge in
the preparation of transmission electron microscope (TEM) thin specimens
(preferably from fine-grained sediment/soil specimens and /or other
geological samples). 2) Knowledge in the operation of TEMâs and
scanning electron microscopes (SEMâs) and their analytical detectors.
3) Knowledge in the basic interpretation of TEM data. 4) Ability to
communicate technical concepts orally and in writing.

The Department of the Navy is an Equal Opportunity Employer.

For further information contact...

Naval Research Laboratory
Human Resources Office
455 Overlook Avenue SW
Code 1810.BMS
Washington, DC 20375-5320
(202) 767-3030

or visit the web page http://www.nrl.navy.mil/hro.htm

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Wed May 17 21:57:22 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 17 May 2000 07:46:42 -0700
Subject: Re: (Fwd) Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:57 PM 5/16/00, you wrote:


} [snip]


} If you control the scan you only need a single light sensitive
} element. You step the beam digitize the light, step the beam,
} wait for the last spot to go out and repeat. You don't need
} a camera. You do need a very fast responding system for
} changing electron beams to light.
}
} This system has the resolution of the scan beam. It would not
} be particualy fast but you could increase the number of bits
} resolution to as large a number as you wanted.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00

The light sensitive element is already in-place. It is the
Everhart-Thornley scintillator detector, or any other
SE or BSE detector on a SEM. Just passively tap into
the record CRT signals, digitize the detected video
(SE/BSE), and save the resulting image as a digital
file. Changing scan rates will change pixel dwell time.
But the final image ought to still be a digital representation
of what was intended to go to the film/Polaroid camera.

Again, the other option is active control of the SEM's beam.
This just swaps the internal scan generator with an external
one. The video system and digitization is the same as
for a passive system. A robust active system will be capable
of producing higher resolution digital images than a passive
system. This is because a good active system can go up
to 4096 horizontal pixels (12-bit D/A converter) and the
pixel dwell time is usually adjustable. The only down side
is the total frame time based on pixel dimensions and
dwell time. At extremes, one could be waiting a very long
time for a single image. If the beam is not stable, and
as pointed out in an earlier posting, the drive circuits
are not stable, there would be some upper limit on pixel
dimensions and dwell time. Beyond this limit, the image
would appear to shift as the beam shifted during the
capture.

Several of the current generation SEMs have incorporated
digital control and digital image capture. But these SEMs
are of course at today's prices. Its rather easy to breathe
new life into an older SEM by adding active or passive
third party digital control/capture systems. One gets
essentially a "new" modern SEM at a fraction of the cost
of buying a new one.

gary g.



From daemon Wed May 17 21:57:33 2000



From: Diane Curley :      Diane.Curley-at-unilever.com
Date: Wed, 17 May 2000 10:51:35 -0500
Subject: Freeze Fracture System

Contents Retrieved from Microscopy Listserver Archives
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We are exploring the possibility of purchasing a freeze fracture system to do
FFTEM of emulsions/microemulsions. Does anyone have any recommendations? Does
anyone have a used system available?

Thank you,
Diane



From daemon Wed May 17 21:57:31 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 17 May 2000 08:56:24 -0700 (PDT)
Subject: Re: mounting clay-rich rocks - soils for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello Nicholas,
I've looked at several soils similar to your easily disaggregated clay
rich rocks in the TEM and find that the best procedure
is to imbed aggregates in 2% agar to hold the aggregates together. The
aggregates are then cut out as agar-soil cubes and chemical processing
(fixation, Os-fix, buffer rinse, dehydration, resin-solvent washes, resin
infiltration) done directly onto the agar cubes. The cubes are placed
into molds and after curing, microtomed to 40-60nm sections. The sections
come out nicely, but if you have a high primary mineral content (quartz,
feldspars } 15%) then you'll have a lot of torn sections. Use of a
diamond knife rather than glass gives you significantly better results.

My methods used in my thesis are on the web at:
http://wilfred.berkeley.edu/~gordon/PHD
Look particularly at chapter four which concentrates on TEM methods and
results. If it would be of use, I'll be glad to send a cdrom copy of my
thesis to you.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 16 May 2000, N. Hayman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
} I believe to be on track with mounting clay rich rocks (that
} disagregate readily in perturbed water (i.e. shaken or sonicated), but not
} in ethylene glycol or ethanol (at rest). My goal is to mount the samples
} (whole, not seperates) for analysis in TEM. I have been soaking them in
} LR-White at 60C, where fixation occurs without accelerator in less than 24
} hours. I am planning to (but have not yet) microtome the samples, or
} potentially ion-mill.
} Thoughts for the future are to use a set of glassware with a
} Millipore filter and vacuum to pull ethanol(cleanser and dilator),
} ethylene glycol(for swelling clay component), and an LR White "chaser" to
} view "saturated" textures, as opposed to compacted.
} My question is this: Can anyone point out pitfalls with this
} approach that I am not seeing, again I haven't tried the whole thing yet,
} but am in a position to start prepping the samples. In particular, is
} microtoming preferred to ion-milling for weak, soft samples that rely on
} epoxy for reinforcement? Does LR-white readily pull through a sample given
} a weak vacuum and a porous plate (its viscous, but not as viscous as
} water, for example)? Does swelling the clays with ethylene glycol and the
} like, and then directly mounting, introduce volatiles into the column
} under 120-200 kV? Any recommendations are welcome, the literature helps a
} bit, but is usually sketchy about these fine details of preparation
} procedure.
} thanks in advance,
} N
}
} _____________________________
} Nicholas W. Hayman \
} Dept. of Geological Sci., U.W.\ http://www.geology.washington.edu/~hayman
} Box 351310, Seattle WA 98195 \_________________________________________
}
}
}
}



From daemon Wed May 17 21:57:36 2000



From: Martin J. Roe :      m.roe-at-mluri.sari.ac.uk
Date: Wed, 17 May 2000 18:10:30 +0000
Subject: EDS mineral identification and database

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
Does anyone know of an EDS mineral spectrum database or
mineral identification program? Is there such a thing available?
Possibly a program that would allow the input of an image file of
the unknown mineral spectrum thereby generating a list of possible
'best-fit' candidates. Or maybe it would work by predicting what the
spectrum of a certain mineral should look like for a particular beam
voltage etc.
Regards
Martin Roe


Martin J. Roe
Macaulay Land Use Research Institute
Craigiebuckler
Aberdeen
Scotland
UK
Phone 01224 318611
e-mail m.roe-at-mluri.sari.ac.uk



From daemon Wed May 17 21:57:42 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 17 May 2000 12:06:17 -0700
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Mickhael.

I will wait for info about sensitivity. I think, for EM in particular,
sensitivity is very important parameter of the system. I am surprised that
manufacturers don't have data on this matter. If sensitivity, say 10 times
higher than SO-163 film - it may be a great reason to switch from film to
the CCD. The major disadvantage of the CCD cameras for TEM is their price
in my point of view.
Thanks for your respond.

Sergey


} Date: Wed, 17 May 2000 11:34:05 -0600
} From: Michael Bode {mb-at-Soft-Imaging.com}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} X-Mailer: Internet Mail Service (5.0.1457.3)
}
} Sergey,
}
} I will try to find out what we have regarding the relative
} sensitivities. One thing that I can say now is, that we acquire images
} with a 50 or 100 msec exposure of the digital camera when the film
} requires an exposure of about 2 seconds. This would mean a 20 to 40
} times better sensitivity of the CCD camera. But to answer your question
} in more detail would require to compare also the resolution of film and
} camera and that is where it becomes very difficult, as it is not easy to
} determine the resolution of film in terms of spatial resolution and
} dynamic range, as both are interwoven. I will try to find some answers
} for you.
}
} Regarding the linearity: As the CCDs simply count Photons, they have
} almost perfect linearity over their complete dynamic range. Even more so
} for TEMs, where all Photons have the same energy and the quantum
} efficiency does not change from photon to photon. The Phosphor is a part
} of a system that could theoretically introduce some non-linearities.
} However, I did measure the linearity of some TEM camera systems a few
} years back and did not find any significant deviations from linearity.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, May 17, 2000 1:29 AM
} To: Michael Bode
} Subject: RE: new developments in imaging systems?
}
}
} Mickhael hello
}
} I have question for you. I am thinking about adding CCD camera to my
} JEM1200EX. The information I gathered from Internet is not so
} optimistic.
} The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} a
} max as I understand. The price for cheaper camera is about 20-30 K$ -
} much
} more that I expect to spend for "film" process. There are two things
} may
} attract me to the modern CCD camera: dynamic range and sensitivity. I
} am
} pretty sure that dynamic range for CCD itself is a few orders better
} than
} any film available. But what about phosphorus screen? Does it reduce
} dynamic range for the EM images? How dramatic? This is my first
} question.
} The next question is: could you tell me something about sensitivity of
} the
} modern CCD cameras used in EM? I am using dark field imaging at x80K
} magnification and the exposure time for SO-163 (non deluded D-19, 7 min,
} 20oC) is about 2 sec. I called GATAN, but they did not say anything
} useful.
} Could you provide some comparison of your side-mount camera with
} sensitivity of the SO-163 film at condition I mentioned? I will greatly
} appreciate any information in this matter. Thanks. Sergey.
}
}
} } Date: Fri, 12 May 2000 13:46:01 -0600
} } From: Michael Bode {mb-at-Soft-Imaging.com}
} } Subject: RE: new developments in imaging systems?
} } To: "'Microscopy-at-MSA.Microscopy.Com'"
} {Microscopy-at-sparc5.microscopy.com}
} } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } X-Mailer: Internet Mail Service (5.0.1457.3)
} }
} } -----------------------------------------------------------------------
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
}

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed May 17 21:57:48 2000



From: Michelle.Taurino-at-aventis.com
Date: Wed, 17 May 2000 14:33:22 -0500
Subject: Re-embedding paraffin sections in plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any suggestions on re-embedding paraffin sections in epon?
Has anyone worked with the "pop out" method using BEEM capsules?

Any suggestions would be helpful...

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Michelle.Taurino-at-aventis.com
908-231-3357



From daemon Wed May 17 21:58:07 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 17 May 2000 15:44:12 -0500
Subject: RE: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm no digital imaging guru, but I have been following this thread with
interest. This whole discussion has been of interest to me because of the
idea of the passive 4x5 (camera back) "detector" that could provide an
extremely simple user venue for converting those countless analog SEMs into
a very usable digital output format.

We have a 10 year old JEOL 840 microanalysis system that we would like to
see in operation for another 10 years, and I think it may be possible.
However, digital imaging, although "doable", is not "convenient" for us, at
this point for this instrument. Too much time and effort is required in
digital acquisition, maintenance of instrument condition info with the
image, labeling the image, and archiving. Then on top of it all, the
digital image is often not quite as good as the analog Polaroid shot that
also has the Mag, Bar Scale, KeV, WD, and other info permanently
incorporated (We use Type 53 for much of our work.) Don't get me wrong, we
do some digital imaging with it, but "it ain't the same" as using a newer
digital scope with integrated digital control and digital interface. If
someone put such a passive digital detector into a 4x5 camera back-type
mount, and it was capable of passively detecting at least 2500 horizontal
lines and an approximately equivalent vertical resolution, I believe it
would be a huge success with the analog SEMs. The 840 and many other
instruments like it are great conventional SEMs, and such a simple interface
(if affordable) would greatly extend their value. Many of these scanners
have exceptional imaging capability, but often the easiest/best way to
record that impressive image is by Polaroid film.

Is it doable?
Brad Huggins

} ----------
} From: Michael Bode[SMTP:mb-at-Soft-Imaging.com]
} Sent: Wednesday, May 17, 2000 1:36 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'John Foust'
} Subject: RE: SEM: digital 4x5 backs
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Yes, there is.
}
} It's called a "passive" or "listening" digital image acquisition system,
} such as our ADDA II or similar devices from other manufacturers.
} Benefits: fairly easy to set up and use. Disadvantages (as opposed to an
} "active" or "talking" system): You're still limited to what the
} microscope can provide in terms of resolution, dwell time, etc.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: John Foust [mailto:jfoust-at-threedee.com]
} Sent: Tuesday, May 16, 2000 8:06 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: SEM: digital 4x5 backs
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} } It seems to me that no digital camera system would work on a SEM
} } in place of a Polaroid or other film-based output device. Since the
} } recording CRT in a SEM is based on a sequential line scan, one
} } would need a camera that would capture each line as it is produced.
}
} What you're saying is there must be a system out there that
} digitizes that single stream of line scan intensities, then
} processes all that data inside the computer as an image as
} opposed to trying to digitize the frame buffer.
}
} - John
}
}



From daemon Wed May 17 21:58:29 2000



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Thu, 18 May 2000 09:13:57 +1100
Subject: Re: EDS mineral identification and database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear All,
} Does anyone know of an EDS mineral spectrum database or
} mineral identification program? Is there such a thing available?
} Possibly a program that would allow the input of an image file of
} the unknown mineral spectrum thereby generating a list of possible
} 'best-fit' candidates. Or maybe it would work by predicting what the
} spectrum of a certain mineral should look like for a particular beam
} voltage etc.
} Regards

} Martin Roe

Martin,

I know of no publicly available database of mineral EDX spectra or
identification software. However, I believe several commercial EDX systems
have a facility for generating your own database by collecting spectra from
standard mineral samples. The software can then compare a spectrum from an
unknown with those in the database.

One must be very careful of comparing like with like. All instrument
parameters such as kV, tilt, etc, etc must be equivalent for a reasonable
chance of a correct match.

I use the Desk Top Spectrum Analyser (DTSA) program from NIST to simulate
EDX spectra from minerals, ceramics etc from the known composition. It
requires a fair bit of effort to master the program but I feel it is worth
the effort. Of course it does a lot more than just simulate spectra,
including analysis of real measured spectra with a huge amount of
flexibility in choosing analysis parameters.

DTSA is available free from the NIST web site:

http://www.cstl.nist.gov/div837/837.02/dtsa.html

Hope this helps,






Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.




From daemon Wed May 17 21:58:58 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 17 May 2000 21:13:23 -0400
Subject: Re: Facility manager's meeting

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Debby,
Please include me in any meetings planned to discuss EM lab management.
Rosemary



From daemon Wed May 17 21:58:59 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 17 May 2000 22:02:51 -0400
Subject: RE: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brad:

For what it's worth - if anything, there is a company out here in
California called Silicon Film Technologies that has developed a technology
that converts a traditional 35mm SLR camera into a digital camera. You
essentially just replace the film with something they call (e)film. I
don't know if they have any interest in developing something for this
application, but it may be worth a look. It's a pretty nifty concept for
people who want to do both traditional film photography and also digital
photography with the same equipment.

I have copied a blurb from their website to give you an overview of what
they do:

"Our EFS product suite is a digital photo system comprised of an electronic
film cartridge and a carrier/adapter. The user simply inserts the
cartridge, called (e)film, into the film cavity in the back of a
conventional 35mm SLR camera. After recording up to 24 images, the
cartridge is placed into a carrier/adapter, called (e)port, which may then
be connected directly to a personal computer, enabling images to be
downloaded quickly into the computer and then printed, sent via email, or
modified into a photo end product using photo management software such as
Adobe PhotoShop LE, which comes bundled with the EFS system. We will also
market a digital photo storage module, called (e)box, which can store and
transport hundreds of digital images in the field
when the photographer does not have access to a computer.

The EFS system transforms conventional camera equipment into a digital
image capture system. It is the only system currently available that
provides the convenience and flexibility of choosing between conventional
and electronic film formats
with the same camera body. The product is aimed toward the large, installed
base of 35mm camera owners who would like to enjoy the benefits of digital
imaging while not giving up the cameras, lenses, and photo accessories with
which they are
familiar."

You can check out their website for more information at
www.siliconfilm.com.

DISCLAIMER: I do own a small amount of stock in their parent company,
Irvine Sensors Corporation. Of course, even if all of you bought a dozen
of their neat little cameras, I'd still have to keep my day job, but I
thought I should disclose it.

Best regards-

David
Writing at 4:13:48 PM on 05/17/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "Huggins, Bradley J"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm no digital imaging guru, but I have been following this thread with
interest. This whole discussion has been of interest to me because of the
idea of the passive 4x5 (camera back) "detector" that could provide an
extremely simple user venue for converting those countless analog SEMs into
a very usable digital output format.

We have a 10 year old JEOL 840 microanalysis system that we would like to
see in operation for another 10 years, and I think it may be possible.
However, digital imaging, although "doable", is not "convenient" for us, at
this point for this instrument. Too much time and effort is required in
digital acquisition, maintenance of instrument condition info with the
image, labeling the image, and archiving. Then on top of it all, the
digital image is often not quite as good as the analog Polaroid shot that
also has the Mag, Bar Scale, KeV, WD, and other info permanently
incorporated (We use Type 53 for much of our work.) Don't get me wrong,
we
do some digital imaging with it, but "it ain't the same" as using a newer
digital scope with integrated digital control and digital interface. If
someone put such a passive digital detector into a 4x5 camera back-type
mount, and it was capable of passively detecting at least 2500 horizontal
lines and an approximately equivalent vertical resolution, I believe it
would be a huge success with the analog SEMs. The 840 and many other
instruments like it are great conventional SEMs, and such a simple
interface
(if affordable) would greatly extend their value. Many of these scanners
have exceptional imaging capability, but often the easiest/best way to
record that impressive image is by Polaroid film.

Is it doable?
Brad Huggins {



From daemon Wed May 17 21:59:02 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 18 May 2000 14:15:02 +1000
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Martin:
An interesting, but difficult idea. Besides the problem of instrument
parameters, and settings, there is the problem of mineral compositions being
in many cases very similar, but having different structures. For example,
there are several iron oxide/hydroxide phases that would be difficult to
tell apart with an EDS analysis. More complex are the silicates which have a
number of different major structural families, many having the same or
similar chemical compositions. There are sites on the web that would allow
you to input an element list and it will output all the minerals with those
elements and their formulas. It won't identify the minerals, but it would
give you a start. To identify them you need to do optical or x-ray
diffraction or some other more appropriate technique.
Michael L. Boucher Sr.
mboucher-at-isd.net
http://www.isd.net/mboucher
-----Original Message-----
} From: Martin J. Roe {m.roe-at-mluri.sari.ac.uk}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}


If sensitivity was ten times higher, one would in some cases save some beam
damage and in most cases suffer excessive electron noise.
As a rough guide, electron noise becomes apparent at 30x enlargement, rarely a
problem. Magnification is a linear function and electron density relates to an
area, but clearly very short exposure images would be much noisier and could
not be enlarged nearly as much. When not enough electrons form an image it
appears grainy, which makes it unsuitable for further enlarging. Photo
enlarging utilises higher depths-of-field and without this, very high power TEM
is much, much harder.
By nature, slower emulsions have finer grain and higher resolution and
contrast. It is fortuitous that these desirable characteristics run in tandem
with relatively long exposures, so the image is formed by more electrons.
TEM films rate about 6 ISO, so X-ray film at about ISI 3000 is about a 10x
faster than TEM films. Unfortunately its rather grainy, but if the exposure was
well adjusted, chances are that electron noise would be more bothersome than
the film's grain.
Digital is inevitable and already fairly common, but some aces remain with
conventional film.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, May 18, 2000 5:06 AM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Thank you Mickhael.
}
} I will wait for info about sensitivity. I think, for EM in particular,
} sensitivity is very important parameter of the system. I am surprised that
} manufacturers don't have data on this matter. If sensitivity, say 10 times
} higher than SO-163 film - it may be a great reason to switch from film to
} the CCD. The major disadvantage of the CCD cameras for TEM is their price
} in my point of view.
} Thanks for your respond.
}
} Sergey
}
}
} } Date: Wed, 17 May 2000 11:34:05 -0600
} } From: Michael Bode {mb-at-Soft-Imaging.com}
} } Subject: RE: new developments in imaging systems?
} } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} } X-Mailer: Internet Mail Service (5.0.1457.3)
} }
} } Sergey,
} }
} } I will try to find out what we have regarding the relative
} } sensitivities. One thing that I can say now is, that we acquire images
} } with a 50 or 100 msec exposure of the digital camera when the film
} } requires an exposure of about 2 seconds. This would mean a 20 to 40
} } times better sensitivity of the CCD camera. But to answer your question
} } in more detail would require to compare also the resolution of film and
} } camera and that is where it becomes very difficult, as it is not easy to
} } determine the resolution of film in terms of spatial resolution and
} } dynamic range, as both are interwoven. I will try to find some answers
} } for you.
} }
} } Regarding the linearity: As the CCDs simply count Photons, they have
} } almost perfect linearity over their complete dynamic range. Even more so
} } for TEMs, where all Photons have the same energy and the quantum
} } efficiency does not change from photon to photon. The Phosphor is a part
} } of a system that could theoretically introduce some non-linearities.
} } However, I did measure the linearity of some TEM camera systems a few
} } years back and did not find any significant deviations from linearity.
} }
} } Michael
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} } Sent: Wednesday, May 17, 2000 1:29 AM
} } To: Michael Bode
} } Subject: RE: new developments in imaging systems?
} }
} }
} } Mickhael hello
} }
} } I have question for you. I am thinking about adding CCD camera to my
} } JEM1200EX. The information I gathered from Internet is not so
} } optimistic.
} } The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} } a
} } max as I understand. The price for cheaper camera is about 20-30 K$ -
} } much
} } more that I expect to spend for "film" process. There are two things
} } may
} } attract me to the modern CCD camera: dynamic range and sensitivity. I
} } am
} } pretty sure that dynamic range for CCD itself is a few orders better
} } than
} } any film available. But what about phosphorus screen? Does it reduce
} } dynamic range for the EM images? How dramatic? This is my first
} } question.
} } The next question is: could you tell me something about sensitivity of
} } the
} } modern CCD cameras used in EM? I am using dark field imaging at x80K
} } magnification and the exposure time for SO-163 (non deluded D-19, 7 min,
} } 20oC) is about 2 sec. I called GATAN, but they did not say anything
} } useful.
} } Could you provide some comparison of your side-mount camera with
} } sensitivity of the SO-163 film at condition I mentioned? I will greatly
} } appreciate any information in this matter. Thanks. Sergey.
} }
} }
} } } Date: Fri, 12 May 2000 13:46:01 -0600
} } } From: Michael Bode {mb-at-Soft-Imaging.com}
} } } Subject: RE: new developments in imaging systems?
} } } To: "'Microscopy-at-MSA.Microscopy.Com'"
} } {Microscopy-at-sparc5.microscopy.com}
} } } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } } X-Mailer: Internet Mail Service (5.0.1457.3)
} } }
} } } -----------------------------------------------------------------------
} } -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} }
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------
} } .
} } }
} } }
} } } Margaret,
} } }
} } } As a former user and current vendor of such systems as you are
} } inquiring
} } } about I can try to provide a bit of information regarding camera
} } } improvements:
} } }
} } } There have been a number of improvements, but I am not sure what you
} } are
} } } comparing the latest cameras against. Cameras are now usually cooled
} } and
} } } provide 12 bits per pixel, the number of pixels has gone up a bit (but
} } } not much in general), and cameras read out faster than they used to (up
} } } to 20 fps and more). I think all cameras now use a line transfer
} } } mechanism, which makes shutters obsolete.
} } } On the software side, real-time FFT and real-time shading correction
} } can
} } } be done now due to faster computers without special processing boards,
} } } and there have been other software developments that make using the
} } } cameras and computers easier.
} } } Other changes that affect the usability of cameras is the use of
} } } pneumatics to insert and retract the phosphors, higher frame rates for
} } } live viewing with the camera, etc.
} } }
} } } If you have questions, please give me a call, drop me an email, or go
} } to
} } } our web site.
} } }
} } } Michael
} } }
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } }
} } } -----Original Message-----
} } } } From: Margaret Dienelt [mailto:brannign-at-asrr.arsusda.gov]
} } } Sent: Thursday, May 11, 2000 12:43 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: TEM: new developments in imaging systems?
} } }
} } }
} } } -----------------------------------------------------------------------
} } -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} }
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------
} } .
} } }
} } }
} } } Hi,
} } }
} } } Year after year I hopefully gather information about digital imaging
} } } systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
} } } money. This year it looks like it might really happen but I have not
} } } kept up with innovations in the field and am wondering the following:
} } }
} } } 1. Anything new in the last two years -- especially in terms of
} } } cameras? I'm most familiar with the Gatan and AMT systems but their
} } } web sites don't reflect much in the way of changes over a year ago.
} } } 2. With more and more microscopists finally getting their systems --
} } } I'd love to get feedback.
} } }
} } } Thanks,
} } } Margaret
} } }
} } } P.S. Would welcome contacts from vendors.
} } }
} } } --
} } } Margaret Dienelt
} } }
} } } Plant Pathologist
} } } Electron Microscopy Lab
} } }
} } } Floral and Nursery Plants Research Unit
} } } U.S. National Arboretum/Agricultural Research Service/USDA
} } }
} } } B. 010A, Rm. 238, BARC-W
} } } 10300 Baltimore Avenue
} } } Beltsville MD. 20705 USA
} } }
} } } (301) 504-6097
} } } Fax: (301) 504-5096
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}



From daemon Thu May 18 23:02:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 17 May 2000 22:58:32 -0700
Subject: RE: SEM: digital 4x5 backs

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I can identify with your situation. While I do not know the intricacies
of the JEOL instruments, some SEMs are made to accept external
drive for x-ray analysis. This same input scheme works perfectly
for active control of the SEM and direct digital capture of images.
At the worst, you can replicate the resolution of your record CRT
using a passive mode. How easy either of these modes are depends
greatly on how the SEM system was designed.

gary g.

At 01:44 PM 5/17/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu May 18 23:02:27 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Thu, 18 May 2000 18:28:19 +1000
Subject: Re: EDS mineral identification and database

Contents Retrieved from Microscopy Listserver Archives
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} Dear Martin:
} An interesting, but difficult idea. Besides the problem of instrument
} parameters, and settings, there is the problem of mineral compositions being
} in many cases very similar, but having different structures.

And then there is also the converse: the problem of mineral compositions
being in many cases very different, but having the same structures. One of
a huge number of examples would be the feldspar series where Na and Ca can
substitute for each other continuously from Na to Ca endmembers.

Various other bits of crystallographic information, some intangibles such
as crystal shape and growth habit, associations with other minerals and so
on, would have to be taken into account in such software for it to really
zero in on an unambiguous ID for you.



Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Thu May 18 23:02:52 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Thu, 18 May 2000 10:55:01 +0000
Subject: looking for ETEC Omniscan manuals

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Hello all .....
we have an old ETEC Omniscan SEM, and we need to contact with any
people that can supply manuals ....( fundamentally electric and electronic
schematic )

any help is welcome

best regards


===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Thu May 18 23:03:00 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 18 May 2000 06:27:27 -0700
Subject: RE: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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At 07:02 PM 5/17/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I think that their aim is a bit off the target. Its nice to not have to
give up the hardware but their offering makes the user give up the
benefits of 35mm film--frame size and selection of speed. And,
a field of view factor of 2.8 is absurd.

I beta tested what must have been an initial offering of this
product early last year. It was by a different company. Same idea
though. Same deficiencies. The sensor is 1.3M pixels and
has an odd aspect ratio of 1.25 (35mm frame is 1.5). Also,
and most importantly, the product does not image the entire
35mm frame, only a small central portion. 1.3M pixel
point and shoot cameras are a lot cheaper than this
silicon film thing. A really dedicated user would have to buy
more than one silicon film insert. At $600 each, that would
buy a lot of P&S cameras. But there is no need to do that
since one just pops out a SmartMedia or Compact Flash
module ($150 or so each).

I still say wait and see how the Fuji Finepix S1 Pro performs.
If it lives up to spec, I'd say that it will be a raging success
and a major turning point in digital cameras which are
based on the 35mm format.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu May 18 23:02:46 2000



From: ComCryLab1-at-aol.com
Date: Thu, 18 May 2000 09:36:31 EDT
Subject: TEM: Help

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Dear Friends,
Can anyone give me advice regarding the dismantling and
the packing for shipment of a Philips 201 TEM? I need to
pay particular attention to not disturbing the alignment.
I plan to move the TEM from Univ. of Delaware to Naples, Florida
in an enclosed U-Haul Trailer. Any help or suggestions
will be most welcome.
If anyone has hands-on experience in doing this sort of thing
and is willing to give me a hand at U-Del, please contact me
and name your price. I'll gladly pay for the assistance.
Best regards,
Mike Urbanik
www.crystalguru.com



From daemon Thu May 18 23:03:03 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 18 May 2000 08:42:49 -0500
Subject: RE: SEM: digital 4x5 backs

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I will reiterate my point and that of others. An SEM technically has video
only coming from one point in the image at a time. Sure, you could pay lots
of $$ for a big CCD with the resolution you desire, or you could simply
digitize the one (or more) video signals as the beam scans the screen. You
have the choice of either asserting the x-y positions through active
digital control or you can read them off passively.

From a hardware perspective, it is a much easier (and cheaper) task to
build an active or passive system like those on the market than to build a
4x5 camera back detector. For both systems you would still need the
software and computer

At 10:58 PM 5/17/2000 -0700, Gary Gaugler wrote:

} I can identify with your situation. While I do not know the intricacies
} of the JEOL instruments, some SEMs are made to accept external
} drive for x-ray analysis. This same input scheme works perfectly
} for active control of the SEM and direct digital capture of images.
} At the worst, you can replicate the resolution of your record CRT
} using a passive mode. How easy either of these modes are depends
} greatly on how the SEM system was designed.
}
} gary g.
}
} At 01:44 PM 5/17/00, you wrote:
} }
} } I'm no digital imaging guru, but I have been following this thread with
} } interest. This whole discussion has been of interest to me because of the
} } idea of the passive 4x5 (camera back) "detector" that could provide an
} } extremely simple user venue for converting those countless analog SEMs into
} } a very usable digital output format.
} }
} } We have a 10 year old JEOL 840 microanalysis system that we would like to
} } see in operation for another 10 years, and I think it may be possible.
} } However, digital imaging, although "doable", is not "convenient" for us, at
} } this point for this instrument. Too much time and effort is required in
} } digital acquisition, maintenance of instrument condition info with the
} } image, labeling the image, and archiving. Then on top of it all, the
} } digital image is often not quite as good as the analog Polaroid shot that
} } also has the Mag, Bar Scale, KeV, WD, and other info permanently
} } incorporated (We use Type 53 for much of our work.) Don't get me wrong, we
} } do some digital imaging with it, but "it ain't the same" as using a newer
} } digital scope with integrated digital control and digital interface. If
} } someone put such a passive digital detector into a 4x5 camera back-type
} } mount, and it was capable of passively detecting at least 2500 horizontal
} } lines and an approximately equivalent vertical resolution, I believe it
} } would be a huge success with the analog SEMs. The 840 and many other
} } instruments like it are great conventional SEMs, and such a simple interface
} } (if affordable) would greatly extend their value. Many of these scanners
} } have exceptional imaging capability, but often the easiest/best way to
} } record that impressive image is by Polaroid film.
} }
} } Is it doable?
} } Brad Huggins



From daemon Thu May 18 23:02:54 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Thu, 18 May 2000 07:07:38 -0700
Subject: EDS and Mineral ID

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Hi Martin,

I fuss with mineral identification via EDS spectra every day. I keep
the program "Mineral" running at all times. It is a Windows Filemaker
Pro database which includes the chemical formula, physical properties,
type locale and 7 to 10 x-ray diffraction lines for 4500 accredited
mineral species and many un-named ones. Searches can be constructed
from any single or combination of fields. The software is available
from Aleph Enterprises in Livermore, CA (510 443 7319) The price was
around $550 a few years ago. Less expensive DOS based, but similar
software is available from the Fersmann Institute.

More complete chemistry (but no spectra) is offered by Alexander
Holzel's MDAT program. It includes a database of chemical analyses of
many minerals and can perform a search based on weight per cents of
elements present as well the unknown's physical properties and x-ray
diffraction data. This software is $1000 and up depending upon
options. Dr. Holzel's e-mail address was Compuserve100333,2771 as of
last August. He is in Ober-Olm, Germany.

None of the software programs work directly from spectra and only MDAT
contains sample analyses so you will likely be estimating peak heights
from chemical formula. For more definitive results you will need to
start building your own spectrum library from your own reference
materials.

Even with a high elemental analysis correlation, one will almost always
need supplemental methods for a definitive ID. This is usually x-ray
diffraction or optical microscopy.

Some day affordable EBSP retrofitted into SEMS will allow the analyst to
distinguish, in situ in polished section between orthorhombic FeS2
(marcasite) and cubic FeS2 (pyrite). Currently available systems are
$90,000+, I think.

If you need help obtaining grains of some of the less common minerals, I
can help. I supply a catalog which includes hundreds of well identified
reference quality mineral grains in addition to synthetic probe
standards.


Bart Cannon
Cannon Microprobe
1041 NE 100th Street
Seattle, WA 98125
206 522 9233 (3947 fax)



From daemon Thu May 18 23:03:08 2000



From: Sinkler, Wharton :      wharton.sinkler-at-anlw.anl.gov
Date: Thu, 18 May 2000 08:58:50 -0600
Subject: JEOL 2010: Electron dose from small screen reading

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Dear List,

In evaluating some electron beam damage data, I am trying to determine the
electron dose to the sample using a small screen current reading from a JEOL
2010 TEM.

The screen current density is given in pA/cm^2 on the microscope terminal,
and can be converted to dose (electrons/cm^2*s) in the sample. I have
spoken to JEOL, and they indicated that the actual current density on the
screen is the reading multiplied by a factor of 10.

I am unable to use a borrowed stage with a Farraday cup for calibration on
this microscope because it is suspect for internal radioactive
contamination. Thus, I would be grateful for insights from other users of
2010's who may have checked the accuracy of the reading (whether or not the
factor of 10 is right, etc.) on their instruments.

Many Thanks,
Wharton
--
++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler
Argonne National Laboratory West
P. O. Box 2528
Idaho Falls, ID 83403
Tel: (208) 533-7724



From daemon Thu May 18 23:03:10 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Thu, 18 May 2000 11:15:41 -0400
Subject: Re: EDS mineral identification and database

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Dear Martin,
As the other responders have stated, minerals can be difficult to identify
with EDS only. There is no substitute for experience (and some diffraction
data). My rule of thumb is to group minerals as do most mineral
classification schemes; is it a silicate, oxide, carbonate, sulfide,
phosphate, salt, organic, or metalic phase? Sometimes it's hard to decide
even at this level (i.e., If you don't have a light element detector, you
can't differentiate between oxides and carbonates, P and Si substitute for
each other). Most times you can. Since I often work with silicates, my
second observation is to look at Si/Al. This will help limit the choices
greatly, but is not usually diagnostic by itself. There are relatively few
minerals with Al} Si. Knowledge of the approximate Si/Al (the most common
tetrahedral cations) combined with the ratio of Si and Al with other
elements like Mg, Ti, Cr, Mn and Fe (sometimes Ca) will give you an idea of
the tetrahedral to octahedral ratio. The alkali elements Na and K, and also
Ca in significant abundances are very important for identifying feldspars
and sheet silicates, but again, there are no hard and fast rules that I've
come up with. The best thing is to know your mineral compositions (and
their structures) or hire a mineralogist!;-) I also agree that even the
mineralogist can find the DTSA program very useful and worth investing in a
MAC. Hope this helps.
Ciao for now,
Ken

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
Johns Hopkins University
Baltimore, Maryland 21218 USA
Phone: (410) 516-8342
Fax: (410) 516-7933
e-mail: klivi-at-jhu.edu




From daemon Thu May 18 23:03:15 2000



From: Anpatoponiopo-at-aol.com
Date: Thu, 18 May 2000 11:51:32 EDT
Subject: LM - Need help choosing a new microscope.

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Due to the price of today's microscopes I am in need of advice concerning the
quality of Russian microscopes. I had one in Spain and it was excellent.
Please, advice.
Thanks, Jose



From daemon Thu May 18 23:03:19 2000



From: Winnie Westbrook :      ewwestbr-at-hsc.vcu.edu
Date: Thu, 18 May 2000 12:37:58 -0400
Subject: EM Safety Chart

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Hello Folks,
In reading the third edition of Hayat's Principles and Techniques of
Electron Microscopy, there was mention of a 'Safety Chart, Chemicals in
Electron Microscopy', for free distribution, by EMscope Laboratories Ltd
(Kingsnorth Industrial Estate, Ashford, Kent). Does anyone out there
have it? Or can anyone tell me how to get it or any wall chart that
lists chemicals/hazards specific to EM?
Thanks,
Winnie



From daemon Thu May 18 23:04:06 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Thu, 18 May 2000 13:47:52 -0400
Subject: Re: EDS mineral identification and database

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Lou,
Very true. However, not one instrument can unambigously identify ALL
minerals! In the case of my interests, PLM is not very useful since most of
the crystals in shale are less than 2 um. Martin's original question was
regarding how to identify EDS patterns, not what is the best way to
identify a mineral.
Ciao for now,
Ken

} Colleagues;
}
} My question is why have you forgotten that polarized light microscopes were
} devised for a need to identify and classify minerals? It almost seems
} intentional to ignore it. Perhaps I am beginning to sound like Dr. McCrone
} as I get older, but you dont have to throw a million dollar instrument at a
} problem to solve it. If people have a problem with a $20k light microscope
} doing a job better, that is there problem not mine. Just dont neglect the
} fact that PLM is still a powerful technique in the hands of a competent
} microscopist. PLM is still the standard for characterizing new crystal
} compounds at the be.....visit any pharmaceutical companys R&D department and
} you will see that it is so.
}
} Lou Solebello
}
}
} ----- Original Message -----
} From: Kenneth JT Livi {klivi-at-jhu.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Cc: {m.roe-at-mluri.sari.ac.uk}
} Sent: Thursday, May 18, 2000 8:15 AM
} Subject: Re: EDS mineral identification and database
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Martin,
} } As the other responders have stated, minerals can be difficult to identify
} } with EDS only. There is no substitute for experience (and some diffraction
} } data). My rule of thumb is to group minerals as do most mineral
} } classification schemes; is it a silicate, oxide, carbonate, sulfide,
} } phosphate, salt, organic, or metalic phase? Sometimes it's hard to decide
} } even at this level (i.e., If you don't have a light element detector, you
} } can't differentiate between oxides and carbonates, P and Si substitute for
} } each other). Most times you can. Since I often work with silicates, my
} } second observation is to look at Si/Al. This will help limit the choices
} } greatly, but is not usually diagnostic by itself. There are relatively few
} } minerals with Al} Si. Knowledge of the approximate Si/Al (the most common
} } tetrahedral cations) combined with the ratio of Si and Al with other
} } elements like Mg, Ti, Cr, Mn and Fe (sometimes Ca) will give you an idea
} of
} } the tetrahedral to octahedral ratio. The alkali elements Na and K, and
} also
} } Ca in significant abundances are very important for identifying feldspars
} } and sheet silicates, but again, there are no hard and fast rules that I've
} } come up with. The best thing is to know your mineral compositions (and
} } their structures) or hire a mineralogist!;-) I also agree that even the
} } mineralogist can find the DTSA program very useful and worth investing in
} a
} } MAC. Hope this helps.
} } Ciao for now,
} } Ken
} }
} } Kenneth JT Livi
} } Department of Earth and Planetary Sciences
} } 34th and Charles Streets
} } Johns Hopkins University
} } Baltimore, Maryland 21218 USA
} } Phone: (410) 516-8342
} } Fax: (410) 516-7933
} } e-mail: klivi-at-jhu.edu
} }
} }
} }





From daemon Thu May 18 23:04:10 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 18 May 2000 14:48:44 -0400
Subject: Ladd Web Site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, we are aware of the problems in reaching our web site in the last few days. We have
posted our new web site of microscopy supplies at a new URL and it is now up and
running. Please change your bookmarks to:

http://www.laddresearch.com

Thank you,

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com



From daemon Thu May 18 23:04:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 18 May 2000 14:33:54 -0700
Subject: Digitally enabling SEMs

Contents Retrieved from Microscopy Listserver Archives
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Warren's point is right on. The JEOL 840 or any other SEM
that has a record CRT for 4x5 film or Polaroid is the signal source
of passive information. It is not a big technical deal to do this.
Depending on how readily available the CRT signals are (blank, frame,
etc.), it may not be convenient or really simple. Some systems
use BNC connectors to snake the signals throughout the system.
T-ing off of these makes digitizing the SEM quite easy. If the
signals are hardwired, it is more work to install the capture
system. But in either case, it is a one time effort.

A passive system will record all legends just like the Polaroid
does. But it will do it a lot better. The reason is that the
tonal range and exposure latitude of a Polaroid is rather poor
compared to real film. Digital capture systems are typically
10-bits; or 12-bits for ones with really exceptional dynamic range.
Either of these are vastly superior to Polaroid prints.

The number of horizontal lines that a passive system will
capture is the same as a Polaroid. This is because the
record CRT circuitry fixes this dimension. However, the
scan rate alters the pixel dwell time. Slower scan rates
produce images that have less noise--be it Polaroid or
digital capture. If the Polaroid print works OK for you,
I would suggest that a digital capture system would be
even better.

Compared to the cost of a modern computerized SEM,
a digitizing attachment can be the key factor in keeping
a good old SEM.

gg

At 06:42 AM 5/18/00, you wrote:

} I will reiterate my point and that of others. An SEM technically has video
} only coming from one point in the image at a time. Sure, you could pay
} lots of $$ for a big CCD with the resolution you desire, or you could
} simply digitize the one (or more) video signals as the beam scans the
} screen. You have the choice of either asserting the x-y positions through
} active digital control or you can read them off passively.
}
} From a hardware perspective, it is a much easier (and cheaper) task to
} build an active or passive system like those on the market than to build
} a 4x5 camera back detector. For both systems you would still need the
} software and computer
}
} At 10:58 PM 5/17/2000 -0700, Gary Gaugler wrote:
}
} } I can identify with your situation. While I do not know the intricacies
} } of the JEOL instruments, some SEMs are made to accept external
} } drive for x-ray analysis. This same input scheme works perfectly
} } for active control of the SEM and direct digital capture of images.
} } At the worst, you can replicate the resolution of your record CRT
} } using a passive mode. How easy either of these modes are depends
} } greatly on how the SEM system was designed.
} }
} } gary g.
} }
} } At 01:44 PM 5/17/00, you wrote:
} } }
} } } I'm no digital imaging guru, but I have been following this thread with
} } } interest. This whole discussion has been of interest to me because of the
} } } idea of the passive 4x5 (camera back) "detector" that could provide an
} } } extremely simple user venue for converting those countless analog SEMs into
} } } a very usable digital output format.
} } }
} } } We have a 10 year old JEOL 840 microanalysis system that we would like to
} } } see in operation for another 10 years, and I think it may be possible.
} } } However, digital imaging, although "doable", is not "convenient" for us, at
} } } this point for this instrument. Too much time and effort is required in
} } } digital acquisition, maintenance of instrument condition info with the
} } } image, labeling the image, and archiving. Then on top of it all, the
} } } digital image is often not quite as good as the analog Polaroid shot that
} } } also has the Mag, Bar Scale, KeV, WD, and other info permanently
} } } incorporated (We use Type 53 for much of our work.) Don't get me wrong, we
} } } do some digital imaging with it, but "it ain't the same" as using a newer
} } } digital scope with integrated digital control and digital interface. If
} } } someone put such a passive digital detector into a 4x5 camera back-type
} } } mount, and it was capable of passively detecting at least 2500 horizontal
} } } lines and an approximately equivalent vertical resolution, I believe it
} } } would be a huge success with the analog SEMs. The 840 and many other
} } } instruments like it are great conventional SEMs, and such a simple interface
} } } (if affordable) would greatly extend their value. Many of these scanners
} } } have exceptional imaging capability, but often the easiest/best way to
} } } record that impressive image is by Polaroid film.
} } }
} } } Is it doable?
} } } Brad Huggins
}



From daemon Thu May 18 23:04:26 2000



From: prenitzer,brenda s :      prenitzer-at-lucent.com
Date: Thu, 18 May 2000 19:06:25 -0400
Subject: Room Temperature TEM Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I am in need of a way to modify my current method of preparing TEM
cross-sections as to introduce no heating. Specifically, I need to find new
adhesive materials. We currently use thermally cured M-bond, Gatan G1 epoxy
and crystal bond wax - all of which require heat.

If anyone has information on an adhesive that ion mills at a rate to similar
Si, is stable under the electron beam, will cure at room temperature within
about 24 hours, and once cured is impervious to solvents such as acetone,
please respond. It would be an additional plus if the adhesive has low
viscosity, so it can be used to secure TEM grids to the specimens.


Also I am looking for a replacement for the wax that we currently use to
affix the samples to the polishing studs. This should cure quickly, bond
strongly enough to endure the mechanical stresses of grinding, and be
readily soluble in a solvent other than water so the samples can be removed
from the studs.

Any help would be greatly appreciated.

Brenda

Brenda I. Prenitzer, Ph.D.
Member of Technical Staff
Cirent Semiconductor (Lucent Technologies)
9333 S. John Young Parkway
6D-Lab
Orlando, FL 32819-8612

Phone: 407 371 7108
Fax: 407 371 6999

prenitzer-at-lucent.com
bsp101-at-worldnet.att.net



From daemon Thu May 18 23:04:28 2000



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Fri, 19 May 2000 10:49:39 +1000
Subject: Our management has finally agreed to connect my electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our management has finally agreed to connect my electron microscopes to
a recirculating water system.

I need some ideas what type of systems are on the market and the
Australian agent for these units. We need to attach 2 SEMs, 1 TEM plus 1
coating unit to the new reciculating line.

Thank you

Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Thu May 18 23:04:28 2000



From: Mike Bode :      MikeB-at-Soft-Imaging.com
Date: Thu, 18 May 2000 19:08:31 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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Sergey,

let me give you two more pieces of information:

1) Sensitivity: The CCD cameras are sensitive enough to see single
electrons striking the phosphor. You can't get much more sensitive than
that.

2) Price: I had a discussion about this with George McAuliffe in this
forum about that theme a while ago. That thread was also printed in
Microscopy Today. You may want to check the archives of this list server
for "digital archiving/cost". I think George would agree with me, that
the calculations of cost can swing in one or the other direction,
depending on how you define cost, what you need to include and how many
images you take (remember, a negative is on the order of $1 for the
material alone, not labor time or anything else).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, May 17, 2000 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Thank you Mickhael.

I will wait for info about sensitivity. I think, for EM in particular,
sensitivity is very important parameter of the system. I am surprised
that
manufacturers don't have data on this matter. If sensitivity, say 10
times
higher than SO-163 film - it may be a great reason to switch from film
to
the CCD. The major disadvantage of the CCD cameras for TEM is their
price
in my point of view.
Thanks for your respond.

Sergey


} Date: Wed, 17 May 2000 11:34:05 -0600
} From: Michael Bode {mb-at-Soft-Imaging.com}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} X-Mailer: Internet Mail Service (5.0.1457.3)
}
} Sergey,
}
} I will try to find out what we have regarding the relative
} sensitivities. One thing that I can say now is, that we acquire images
} with a 50 or 100 msec exposure of the digital camera when the film
} requires an exposure of about 2 seconds. This would mean a 20 to 40
} times better sensitivity of the CCD camera. But to answer your question
} in more detail would require to compare also the resolution of film and
} camera and that is where it becomes very difficult, as it is not easy
to
} determine the resolution of film in terms of spatial resolution and
} dynamic range, as both are interwoven. I will try to find some answers
} for you.
}
} Regarding the linearity: As the CCDs simply count Photons, they have
} almost perfect linearity over their complete dynamic range. Even more
so
} for TEMs, where all Photons have the same energy and the quantum
} efficiency does not change from photon to photon. The Phosphor is a
part
} of a system that could theoretically introduce some non-linearities.
} However, I did measure the linearity of some TEM camera systems a few
} years back and did not find any significant deviations from linearity.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, May 17, 2000 1:29 AM
} To: Michael Bode
} Subject: RE: new developments in imaging systems?
}
}
} Mickhael hello
}
} I have question for you. I am thinking about adding CCD camera to my
} JEM1200EX. The information I gathered from Internet is not so
} optimistic.
} The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} a
} max as I understand. The price for cheaper camera is about 20-30 K$ -
} much
} more that I expect to spend for "film" process. There are two things
} may
} attract me to the modern CCD camera: dynamic range and sensitivity. I
} am
} pretty sure that dynamic range for CCD itself is a few orders better
} than
} any film available. But what about phosphorus screen? Does it reduce
} dynamic range for the EM images? How dramatic? This is my first
} question.
} The next question is: could you tell me something about sensitivity of
} the
} modern CCD cameras used in EM? I am using dark field imaging at x80K
} magnification and the exposure time for SO-163 (non deluded D-19, 7
min,
} 20oC) is about 2 sec. I called GATAN, but they did not say anything
} useful.
} Could you provide some comparison of your side-mount camera with
} sensitivity of the SO-163 film at condition I mentioned? I will
greatly
} appreciate any information in this matter. Thanks. Sergey.
}
}
} } Date: Fri, 12 May 2000 13:46:01 -0600
} } From: Michael Bode {mb-at-Soft-Imaging.com}
} } Subject: RE: new developments in imaging systems?
} } To: "'Microscopy-at-MSA.Microscopy.Com'"
} {Microscopy-at-sparc5.microscopy.com}
} } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } X-Mailer: Internet Mail Service (5.0.1457.3)
} }
} } ----------------------------------------------------------------------
-
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
}

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant



From daemon Fri May 19 22:06:37 2000



From: Ranan Gulhan Aktas :      ranaoz-at-usa.net
Date: Thu, 18 May 2000 23:14:06 -0500
Subject: Re: [Re-embedding paraffin sections in plastic]

Contents Retrieved from Microscopy Listserver Archives
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Dear Michelle;

A few months ago, I asked the same question and received the answers I copied
below. By the way, I would like to thank again to all who respond my
question. I do appreciate it. I still use these procedures and am planning
to prepare a study comparing these techniques. All of them work very well.

Hope these helps. Good luck.

Ranan Gulhan AKTAS, M.D.
Trakya University, Faculty of Medicine
Pathology Department
Edirne 22030
TURKEY

Tel: + 90 284 235 76 42
Fax: +90 284 235 76 52
e-mail: ranaoz-at-usa.net


Dear Dr. Ranan Gulhan Aktas
Protocol as follows:
De-wax in 100% xylene - small pieces of tissue - 1mm3 (3X)
Re-hydrate to water
xylene/ethanol 100% each
100% ethanol
96% ethanol
70% ethanol
H2O
GA in buffer} Normal EM processing from here on
Buffer }

Contact me if you have any further queries

Kind regards
John

Mr John F. Putterill
Electron Microscopy Unit Tel: (Int) 27-12-529-9174
Pathology Section Fax: (Int) 27-12-529-9165
Onderstepoort Veterinary Institute E-mail: john-at-moon.ovi.ac.za
Private Bag X05 http://www.ovi.ac.za
Onderstepoort 0110
South Africa

The basic procedure for re-embedding paraffin embedded is relatively
simple. We had a Lynx Tissue Processor set up to do it automatically.
Cut the paraffin tissue into 1-2 mm cubes with a warm scalpel. Trim
as much excess paraffin away as possible. De-paraffinize with 6
alternately warm/room temp xylene washes with agitation for 30 minutes
each. Reverse the dehydration process from 100% EtOH to your normal
EM buffer. Refix and postfix with appropriate EM fixatives,
dehydrate, and embed. The smaller the paraffin pieces, typically the
better the deparaffinization. Your tissue will never look great
though. The initial fixation for LM is a poor TEM fixative. good
Luck.

Chuck Butterick
Engineered Carbons, Inc.

Ranan,

Kai Chein did some nice work in this in the 1970's. He dissolved osmium
in xylene(1 percent)and then deparaffinized in that solution. This took
care of osmicating and depariffinization in one step (assuming you want
to use osmium). After several changes in this solution you can go right
into resin.. I've done this many times and it works very well and saves
a lot of time.


Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9-at-cdc.gov
timcdc-at-hotmail.com

FAX: (404)639-3043

MICROSCOPY AND IMAGING SERVICE CENTER
CELL BIOLOGY AND NEUROSCIENCES

PROCEDURE FOR PRELIMINARY PREPARATION OF PARAFFIN EMBEDDED TISSUE FOR ELECTRON
MICROSCOPY:


1. PLACE 0.5-1.0 mm CUBES OF TISSUE FROM PARAFFIN BLOCK IN XYLENE FOR
3 CHANGES OF 30 MINUTES EACH.

2. PUT IN 100% ETHANOL FOR 2 CHANGES OF 15 MINUTES WITH AGITATION.

3. PUT IN 95% ETHANOL FOR 2 CHANGES FOR 15 MINUTES WITH AGITATION.

4. PUT IN 70% ETHANOL FOR 2 CHANGES FOR 15 MINUTES WITH AGITATION.

5. PUT IN 50% ETHANOL FOR 2 CHANGES FOR 15 MINUTES WITH AGITATION.

6. RINSE IN PBS FOR 2 CHANGES OF 5 MINUTES EACH WITH AGITATION.

7. FOLLOW ROUTINE PREPARATION OF TISSUE FOR ELECTRON MICROSCOPY.

REFERENCE:

PIERCE, A., 1972. "A MANUAL FOR HISTOLOGICAL TECHNICIANS",
LITTLE AND BROWN, BOSTON.

Your morphology will not be great since the tissue was probably fixed in
formalin and
not glut. I've done this many times and have even had publications with this
method.
Make sure you get all of the paraffin out.


George Lawton
Chief Electron Microscopist
Microscopy and Imaging Service Center
UT Southwestern Medical Center at Dallas
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu

Hi: This is Bob Mixon ( I work with Bob Kayton on the PNEMS society and am on
the
OHSU campus) . I have re-embedded tissues from paraffin many times and
many ways. I would recommend cutting out the piece of tissue from the
paraffin block and putting through at least two changes of xylene for
about 30 minutes each. Then you could put the tissue through either
absolute alcohol or acetone to remove the rest of the paraffin.
Probably 30 minutes and two changes. Some folks continue to run
the tissue through a series of alcohol and try to refix in EM fix
and post-fix in osmium. THIS IS FUTILE! I have never found any
enhancement by doing this. You can simply dissolve osmium in the
acetone and try fixing in this (two percent). and then running
through by your routine into plastic (through alcohol, acetone,
or propylene oxide etc).
Thanks Bob Mixon

Hello Ranan.

Here is the method used by the pathology people around here.

- Remove tissue of interest from parafin embedded specimen, cut in 1-2 mm
cubes.
- Leave the specimens in fresh 1% OsO4 in XYLENE for 1,5 - 2 hrs.
- Wash in xylene 2 times 10 min
- One part resin(we use Epon-Araldite) + two parts xylene for 1 hr.
- 1 part resin + 1 part xylene for 1 hr.
- Resin for 30 min to 1 hour without a lid on the glass

All this steps on a carousel.

Embedd as usual, but keep the specimens in the resin over night before
polymerization.

Good luck!

Best regards
Randi Olsen

Randi Olsen
Department of Electron Microscopy
Faculty of Medicine
University of Tromso
MH-Breivika
N-9037 TROMSO
NORWAY

tel: +47 77 64 53 15
fax: +47 77 64 46 50
email: randio-at-fagmed.uit.no

Hi Ranan,

The following procedure may sound a little obvious, but I imagine
you may be facing a rather limited access to all those books and
journals, and I would feel really happy if this could help.
I do wish you all the best in your EM lab-raising mission!

First, you will have to select smaller, "EM-size", portions of your
paraffin-embedded specimens, cut them out and thoroughly deparaffinate.
I would recommend at least 2 hours in xylene with frequent changes of
xylene and some agitation; you should be able to see when it's all gone.
Then several changes of 100% (anhydrous) ethanol, at least 1-1.5 h
total time, and then gradually down the ethanol concentrations, like
95-85-70-50, 30 min or more at each step.
Rinse 3x5 min in your choice of phosphate or cacodylate buffer, and
then osmicate for 1-2 h, 1% OsO4 in the same buffer. Then dehydrate
and embed for EM as you normally would.

Needless to say, even with the best original fixation the material
will look pityful, but most of those diagnostically significant
cell-to-cell junctions, filaments, etc. must still be there.

Best of luck!
Sincerely,
Vlad.

Vladislav V. Speransky
Postdoctoral Research Associate
School of Marine Sciences
University of Maine
5722 Deering Hall
Orono ME 04469-5722
Ph: 207 581 2998
FAX: 207 581 2969
Email: vladis-at-maine.maine.edu


Hi Keith,

At least for the specimens I happened to deal with so far (vertebrate
tissues, cell culture, bacteria, microalgae), the material normally will
not really blacken until you wash and start dehydrating it AFTER
osmication in traditional aqueous OsO4 solutions. While in aqueous OsO4,
it will remain brownish. (Unless, of course, you are using that
simultaneous, single solution glutaraldehyde/OsO4 fixation, or a
reducing buffer like PIPES, and have passed the time when it all
turns black...)

The explanation used to be that, at the stage of osmication, while some
of the OsO4, indeed, oxidizes nonsaturated lipids, etc., a lot of it
simply dissolves in the lipids of the specimen as nonreduced OsO4. Then,
say, the ethanol reduces that specifically accumulated OsO4, to form
the so called "osmium black".

As for making FRESH solutions of OsO4 in xylene (or CCl4, etc.),
I would just never have a spare OsO4 ampule (and enough paraffine
blocks to EM-reevaluate) to try that! :-)

Sincerely,
Vlad.

Vladislav V. Speransky
Postdoctoral Research Associate
School of Marine Sciences
University of Maine
5722 Deering Hall
Orono, ME 04469-5722
Ph: 207 581 2998
FAX: 207 581 2969
Email: vladis-at-maine.maine.edu

} I was interested in the use of osmium in xylene that you described. Does
the tissue blacken as
} in a water-solution or does it simply become yellowish/brown? I am curious
because the latter is
} what happens in the absence of water during freeze-substitution using e.g.
1% in acetone at -80
} *C, although the colour change probably happens when warming up from low
temperature.


Hello Keith.

According to the people here that most often works with this (Irene Lund)
the blocks are not as dark as with standard methods, but light brown. We
haven't done freeze-substitution with osmium, so it's not easy to compare
directly.
For this purpose we buy ampoulas with 0,1 gram OsO4.

Best regards
Randi Olsen
Randi Olsen
Department of Electron Microscopy
Faculty of Medicine
University of Tromso
MH-Breivika
N-9037 TROMSO
NORWAY

tel: +47 77 64 53 15
fax: +47 77 64 46 50
email: randio-at-fagmed.uit.no

Hi Keith,

At least for the specimens I happened to deal with so far (vertebrate
tissues, cell culture, bacteria, microalgae), the material normally will
not really blacken until you wash and start dehydrating it AFTER
osmication in traditional aqueous OsO4 solutions. While in aqueous OsO4,
it will remain brownish. (Unless, of course, you are using that
simultaneous, single solution glutaraldehyde/OsO4 fixation, or a
reducing buffer like PIPES, and have passed the time when it all
turns black...)

The explanation used to be that, at the stage of osmication, while some
of the OsO4, indeed, oxidizes nonsaturated lipids, etc., a lot of it
simply dissolves in the lipids of the specimen as nonreduced OsO4. Then,
say, the ethanol reduces that specifically accumulated OsO4, to form
the so called "osmium black".

As for making FRESH solutions of OsO4 in xylene (or CCl4, etc.),
I would just never have a spare OsO4 ampule (and enough paraffine
blocks to EM-reevaluate) to try that! :-)

Sincerely,
Vlad.

Vladislav V. Speransky
Postdoctoral Research Associate
School of Marine Sciences
University of Maine
5722 Deering Hall
Orono, ME 04469-5722
Ph: 207 581 2998
FAX: 207 581 2969
Email: vladis-at-maine.maine.edu

Dear HistoNetters,

Dr. Aktas asked about the re-embedding of paraffin embedded
tissues in resin for observation by electron microscopy. There
are two papers at our web site which describe this "Pop-Off" technique.
Go to the URL provided in my signature file and follow the links to the
JB-4 microtomy system, then to "Technical Information"

If anyone needs reprints of these articles and has no WWW
access, send a note to Sonja White in my office (sales-at-ebsciences.com)
{mailto:sales-at-ebsciences.com)} , and she'll s-mail you the dead tree
version.

Best regards,
Steven E. Slap, Vice-President

*******************************************
Energy Beam Sciences, Inc.
The Laboratory Microwave Company
Adding Brilliance to Your Vision
http://www.ebsciences.com {http://www.ebsciences.com}
Ranan,

Kai Chein did some nice work in this in the 1970's. He dissolved osmium
in xylene(1 percent)and then deparaffinized in that solution. This took
care of osmicating and depariffinization in one step (assuming you want
to use osmium). After several changes in this solution you can go right
into resin.. I've done this many times and it works very well and saves
a lot of time.


Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9-at-cdc.gov
timcdc-at-hotmail.com

FAX: (404)639-3043
The basic procedure for re-embedding paraffin embedded is relatively
simple. We had a Lynx Tissue Processor set up to do it automatically.
Cut the paraffin tissue into 1-2 mm cubes with a warm scalpel. Trim
as much excess paraffin away as possible. De-paraffinize with 6
alternately warm/room temp xylene washes with agitation for 30 minutes
each. Reverse the dehydration process from 100% EtOH to your normal
EM buffer. Refix and postfix with appropriate EM fixatives,
dehydrate, and embed. The smaller the paraffin pieces, typically the
better the deparaffinization. Your tissue will never look great
though. The initial fixation for LM is a poor TEM fixative. good
Luck.

Chuck Butterick
Engineered Carbons,

Inc."Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Any suggestions on re-embedding paraffin sections in epon?
Has anyone worked with the "pop out" method using BEEM capsules?

Any suggestions would be helpful...

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Michelle.Taurino-at-aventis.com
908-231-3357
"Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Any suggestions on re-embedding paraffin sections in epon?
Has anyone worked with the "pop out" method using BEEM capsules?

Any suggestions would be helpful...

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Michelle.Taurino-at-aventis.com
908-231-3357


____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1




From daemon Fri May 19 22:06:44 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 18 May 2000 22:03:36 -0700
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim hello

I was talking about very special EM case: dark-field TEM. At this point we
are talking about a few electrons per square angstrom, even less. In high
resolution EM crystallography people have deal with 0.6 e/A2. At such
conditions, high sensitivity and linearity of the modern digital cameras
may be a plus. Talking about long exposures --what about drift? I never
had good pictures at x100K with exposure longer that a few seconds. I
don't understand your point about noise. In case of digital camera the
noise is a function of camera. Current cameras has a very low level of
noise (they uses cooling, etc) and we have to pay for that astronomical
price. This is a life. I am not friendly with TEM cameras, but I do know
that in the light microscopy people count individual photons using CCD
cameras. In TEM camera we have phosphorous screen as a source of image and
my questions to Mickhael were addressed mostly to the problem how
effectively (and correctly) information is traveled thought that funny
screen. The Mickhael's answer is that screen does not affect dynamic range
and sensitivity is much higher than on convention films. Am I correct,
Mickhaels?

Sergey

} Date: Thu, 18 May 2000 14:15:02 +1000
} From: jim {jim-at-proscitech.com.au}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri May 19 22:06:49 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Fri, 19 May 2000 11:35:37 +0200
Subject: BSE resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
We have a JEOL 733 with a two-sector PN type BS detector. Anyone out
there have any idea on the resolution of these things in terms of mean
atomic number. What I want to know basically is the sensitivity of these
things to compositional variation.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Fri May 19 22:06:56 2000



From: Martin J. Roe :      m.roe-at-mluri.sari.ac.uk
Date: Fri, 19 May 2000 10:12:33 +0000
Subject: EDS mineralogy/database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all the responses to this posting both on and off the
list.
I routinely analyse minerals found in soils and reservoir
sandstones and can easily recognise most of the common
minerals by their EDS spectra. The problem for me recently
has been looking at samples containing some of the more
'exotic' minerals, or put simply minerals I'm not used to
looking at, e.g. sphene and clinpyroxenes. This has been more
of a problem because we had no supporting XRD data for these
particular analyses.
What I was asking for, perhaps naively, was a program that
would help identify an unknown mineral from its spectrum and
give a list of possible alternatives. This is evidently not
available.
What is available:
Thereis a free Mac program DTSA that can simulate
spectra of a mineral for a particular detector and kV etc.
Scott Wight/Mark Blackford). This can be downloaded
from http://www.cstl.nist.gov/div837/837.02/dtsa.html
Thereis a EDS spectrum database with search
capabilities (based on archived spectra) written for the
US FBI which may be commercially available soon. I
will certainly follow up this up soon (thanks Dennis
Ward and Nicholas Ritchie.)
Thereseems to be various databases including MDAT
that allow a search by chemical elements but not actual
analyses. (thanks Bart Cannon)
About a year ago, I started building a spectrum library
collected on our system recording the various beam
operating parameters, preparation (rough or polished, C or
Au coated) etc. I agree with Bart Cannon that this is
probably the best way forward. Just one thing - my database
needs many more spectra added to it, especially different
variations of many of the solid solution minerals. I suppose
the idea of the database is that it should be used as a
reference guide only. Although some minerals could never
be identified by their EDS spectrum alone this is where an
extensive reference library of my own would come in so
useful and help answer the question: is this spectrum a Ca
feldspar or Ca-rich zeolite? Although the best answer to this
specific question would probably be the unknown spectrum
indicates it may be a Zeolite because it is more similar to
the zeolite reference we collected under similar operating
parameters. Of course there would be no substitute if there
were XRD data telling you in the same sample of zeolite and
no Ca-feldspar.
Thanks again to all of you who responded.
Best regards
Martin Roe

Martin J. Roe
Macaulay Land Use Research Institute
Craigiebuckler
Aberdeen
Scotland
UK
Phone 01224 318611
e-mail m.roe-at-mluri.sari.ac.uk



From daemon Fri May 19 22:07:01 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Fri, 19 May 2000 08:10:49 -0300
Subject: Re: Our management has finally agreed to connect my electronmicroscopes to a recirculating water system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




----------
} From: Hans Brinkies {HBrinkies-at-groupwise.swin.edu.au}
} To: microscopy-at-sparc5.microscopy.com
} Subject: Our management has finally agreed to connect my
electronmicroscopes to a recirculating water system
} Date: May 18, 2000 9:49 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Our management has finally agreed to connect my electron microscopes to
} a recirculating water system.
}
} I need some ideas what type of systems are on the market and the
} Australian agent for these units. We need to attach 2 SEMs, 1 TEM plus 1
} coating unit to the new reciculating line.
}
} Thank you
}
} Hans Brinkies
} Senior Lecturer
} Swinburne, University of Technology
} School of Engineering and Science
} P.O.Box 218 - Hawthorn - Vic -3122 - Australia
} Phone: +61 3 9214 8657
} Fax: +61 3 9214 8264
} Email: Hbrinkies-at-swin.edu.au
}
} I don't really know what companies distribute such systems in Australia,
but one thing occurs to me....if you set up all your instruments on one
circulator, it's nice and cost-effective, but when the pump crashes or
motor burns out, all your toys are down until the one circulation system is
repaired. Separate systems for each 'scope would be better, (that'll
probably give your managers a heart attack) or at least try and retain the
capability of switching back to the old constant-loss system during
emergencies. Our own ESEM was recently down for nearly three weeks while I
was trying to replace the circulator pump motor. Turned out to be kind of a
hard one to find...

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada Atlantic
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Fri May 19 22:07:05 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 19 May 2000 13:37:01 +0100 (GMT Daylight Time)
Subject: Philips heating holders for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

For the past 20 years I have used the Philips TEM heating
holder furnace and heater elements to build hotstages for a
range of microscopes. Unfortunately, Philips have told me
that they can no longer supply the Pt furnace bodies and I
cannot get information on possible replacements.

If anyone has a spare furnace (or more?) - Philips part
number 5322 265 70028 - that they wish to part with I am
willing to pay a `Philips' price. I am also willing to
consider lightly used furnaces or complete holders that are
now surplus to requirements and will pay a price depending
on condition and history.

If anyone has any information on replacements to the
previous style or on present day heaters I would be most
grateful.

Thanks,
Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri May 19 22:07:03 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 19 May 2000 08:03:00 -0500
Subject: Re:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Atomic number contrast sensitivity using BSE is dependent on several
variables
in addition to the equipment. I may not cover everything, but what quickly
comes to mind....

Specimen surface condition - If a specimen is well polished, compo imaging
is
best. Topography of rough specimens (like a fracture) will generate
feature
contrast, "diluting" the compo contrast.

Beam current and potential affect sensitivity.

Conductive films will reduce sensitivity. Use carbon rather than sputtered
Au,
Pd etc. to minimize the effect if the specimen must be coated.

Depending on the detector geometry, working distance can affect the solid
angle
of collection thus changing BSE collection effiency.

The absloute atomic numbers present in the specimen will affect sensitivity
in
two ways.

Sensitivity will be different for a composition of lighter elements compared
to
a specimen composed of higher atomic numbers.

Also, if it is desired to NOT saturate the video, the range of atomic
numbers
can limit sensitivity. This is a typically a result of limited dynamic
range in
the image capture device/photo. For example, if C and W are present as pure
inclusions in a brass, it will be difficult (to say the least) to contrast
the
difference between Cu and Zn phases whild not loosing C & W contrast
variability.

Considering the above, any single statement of sensitivity is difficult, but
if
I had to generalize... My 4 diode GW Electronics BSE system will resolve
0.1
atomic number differencees under "good" conditions.

Woody White
McDermott Technology


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi
We have a JEOL 733 with a two-sector PN type BS detector. Anyone out
there have any idea on the resolution of these things in terms of mean
atomic number. What I want to know basically is the sensitivity of these
things to compositional variation.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA



From daemon Fri May 19 22:07:35 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 19 May 2000 12:28:19 -0400
Subject: RE: Room Temperature TEM Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey and others,

I want to add my 2 cents as I like the topic.
Most of this is a result of my experience with our TEM 2Kx2K CCD.
We are working in bright field - so I cannot comment on dark field
performance
Several points:

1. The CCD is much more convenient - you get your pictures instantly.

2. CCD is linear and has larger dynamic range than the film.

3. The bad thing about the CCD is resolution - about 4 times lower than that
of the film (in our camera the pixel size is 30 microns). So if you want to
work in minimum dose you will do better with film. As I know the CCD is not
performing well in terms of signal to noise at low doses (and if you have to
work at 4 times higher magnification because of the resolution the things
become much worse).

4. The CCD has smaller observation area - again loss of information.

5. I don't know about the detection efficiency compared to the film - it
depends on the thickness of the phosphorous and the accelerating voltage. If
a photon reaches the CCD chip it will be detected ... the problems are in
the conversion electron-photon. There are two sides - if you make the
phosphorous thicker you will get higher detection efficiency but the point
spread also increases so always a compromise is made between detection
efficiency and resolution. When I say detection efficiency this is not only
related to the detection of single electrons (as it detects single
electrons) but more to the actual signal detected on the background of the
noise. Apart from the shot noise additional noise is added due to the
scintillator driven detection and thermal noise in the CCD chip.

The CCDs are now very popular in diffraction work because of the dynamic
range and linearity.

Here is one reference where a nice comparison between 2Kx2K CCD and film has
been made:

Downing, K., Hendrickson, F., Ultramicroscopy, 75, (1999), 215-233

Best regards,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, May 19, 2000 2:03 PM


If your samples are not site specific, then I would recommend a modified
version of the small angle cleavage technique. You can get a detailed
description of this technique in the MRS TEM Sample Prep IV book, vol 480.

Instead of the low temperature melting wax to hold the samples down, you use
superglue. It takes longer to soak the samples off in acetone, but there is
no heat. Then instead of the silver epoxy that is normally used, you need
to use a slow curing viscous epoxy to mount the samples on the copper grid.
There may be a temperature spike in the curing, but you would have to
experiment and find out yourself. The amount of epoxy that is needed is
very small and I doubt that it would raise the temperature an appreciable
amount. The down side is that it will take a day to fully cure the epoxy.
The net result is that the only heating the sample really sees is the heat
generated during grinding the back side of the samples down.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: prenitzer,brenda s [mailto:prenitzer-at-lucent.com]
} Sent: Thursday, May 18, 2000 7:06 PM
} To: 'List Server'
} Subject: Room Temperature TEM Prep
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello all,
}
} I am in need of a way to modify my current method of preparing TEM
} cross-sections as to introduce no heating. Specifically, I
} need to find new
} adhesive materials. We currently use thermally cured M-bond,
} Gatan G1 epoxy
} and crystal bond wax - all of which require heat.
}
} If anyone has information on an adhesive that ion mills at a
} rate to similar
} Si, is stable under the electron beam, will cure at room
} temperature within
} about 24 hours, and once cured is impervious to solvents such
} as acetone,
} please respond. It would be an additional plus if the
} adhesive has low
} viscosity, so it can be used to secure TEM grids to the specimens.
}
}
} Also I am looking for a replacement for the wax that we
} currently use to
} affix the samples to the polishing studs. This should cure
} quickly, bond
} strongly enough to endure the mechanical stresses of grinding, and be
} readily soluble in a solvent other than water so the samples
} can be removed
} from the studs.
}
} Any help would be greatly appreciated.
}
} Brenda
}
} Brenda I. Prenitzer, Ph.D.
} Member of Technical Staff
} Cirent Semiconductor (Lucent Technologies)
} 9333 S. John Young Parkway
} 6D-Lab
} Orlando, FL 32819-8612
}
} Phone: 407 371 7108
} Fax: 407 371 6999
}
} prenitzer-at-lucent.com
} bsp101-at-worldnet.att.net
}
}



From daemon Fri May 19 22:07:48 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Fri, 19 May 2000 11:38:20 -0700
Subject: Amoeba

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am about to do a TEM prep on amoeba. Does anyone out there have any information on a fixation procedure? Thank you!



From daemon Fri May 19 22:07:49 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Fri, 19 May 2000 11:44:18 -0700
Subject: Amoeba

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am about to do a TEM prep on amoeba. Does anyone out there have any information on a fixation procedure? Thank you

Barbara Plowman
University of the Pacific
School of Dentistry
2155 Webster
San Francisco
email: Bplowman-at-sfuop.edu



From daemon Fri May 19 22:07:52 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 19 May 2000 13:27:11 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes,

as I said in my response, a direct comparison between the sensitivities
of film and a CCD camera are very complicated. The film has a non-linear
response curve, it reacts to electrons directly, there is grain size to
take into account, etc. All I wanted to say is the following: For a film
camera setting of 2 seconds, we acquire an image in approx. 50 to 100
msec with similar contrasts to that of film.

Regarding the linearity of the phosphor-CCD system: I have measured the
linearity by measuring the beam current and the image intensity and I
found it to be linear over the entire range that I measured. The
deviations were insignificant and probably due to noise (I would give
you the numbers, but this was in a different life and I don't have
them). There may be nonlinearities that show up if you reach extreme
illumination levels of the phosphor, but I did not measure them and it
will probably depend on the phosphor itself.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Thursday, May 18, 2000 11:04 PM
To: Microscopy-at-sparc5.microscopy.com


Jim hello

I was talking about very special EM case: dark-field TEM. At this point
we
are talking about a few electrons per square angstrom, even less. In
high
resolution EM crystallography people have deal with 0.6 e/A2. At such
conditions, high sensitivity and linearity of the modern digital cameras
may be a plus. Talking about long exposures --what about drift? I
never
had good pictures at x100K with exposure longer that a few seconds. I
don't understand your point about noise. In case of digital camera the
noise is a function of camera. Current cameras has a very low level of
noise (they uses cooling, etc) and we have to pay for that astronomical
price. This is a life. I am not friendly with TEM cameras, but I do
know
that in the light microscopy people count individual photons using CCD
cameras. In TEM camera we have phosphorous screen as a source of image
and
my questions to Mickhael were addressed mostly to the problem how
effectively (and correctly) information is traveled thought that funny
screen. The Mickhael's answer is that screen does not affect dynamic
range
and sensitivity is much higher than on convention films. Am I correct,
Mickhaels?

Sergey

} Date: Thu, 18 May 2000 14:15:02 +1000
} From: jim {jim-at-proscitech.com.au}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri May 19 22:07:58 2000



From: Margaret Mitchell :      MMitchell-at-aoac.org
Date: Fri, 19 May 2000 16:13:45 -0400
Subject: Need Help on microscopy, etc. techniques used to authenticate art

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am new to this list. I would like to know if anyone has information I
could use to write an article on the analytical chemistry techniques used to
authenticate art objects using light microscopes or any other type of
microscope. Also, if anyone has information on any of the other techniques
used to authenticate art objects such as x-ray diffraction, x-ray
fluorescence, accelerator mass spectrometry, that would be helpful too.

Sincerely,

Margaret M. Mitchell
Assistant Editor
AOAC INTERNATIONAL
301-924-7077 (tel)
301-924-7089 (fax)
mmitchell-at-aoac.org



From daemon Fri May 19 22:08:12 2000



From: john david whitaker :      jwhitake-at-u.washington.edu
Date: Fri, 19 May 2000 14:01:02 -0700 (PDT)
Subject: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for information regarding final polishing
solutions for preparing metallic cross-section samples
for TEM analysis.

I've looked into colloidal silica
suspensions such as Ludox and Syton, and am concerned
that their alkilinity may chemically etch my samples.
I'm analyzing different compositions of NiFe
on copper substrates, so the specimens are prone to
preferential etching of different phases.

Does anyone have any experience with this?

Any input would be appreciated.

John



From daemon Fri May 19 22:08:21 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Sat, 20 May 2000 11:31:36 +1000
Subject: Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please reply to email addresses listed in the advertisement. More
information available on:
http://www.anu.edu.au/hr/jobs/ext.html

THE AUSTRALIAN NATIONAL UNIVERSITY
RESEARCH SCHOOL OF BIOLOGICAL SCIENCES
PLANT CELL BIOLOGY GROUP

ANU Officer Grade 7 OR 8 (Research)
Grade 7:$43,506 - $47,010 (Ref. G001411)
Grade 8: $48,696 - $54,146 per annum (Ref. G001410)


Reference: G001410 OR G001411. A position is available for a Head of
Advanced Light Microscopy Facilities in the Biological Sciences at ANU. The
appointee will be responsible for the maintenance of advanced light
microscope equipment in the Research School of Biological Sciences and John
Curtin School of Medical Research and for the training of users of this
equipment. The appointee will be expected to play a major role in the
identification of novel techniques in light microscope imaging and in the
preparation of applications for the procurement of new light microscope
equipment. Experience in biological imaging, computational image processing
and maintenance of opto-electronic systems is required.

The appointment will be made in the Plant Cell Biology Group in the
Research School of Biological Sciences as a continuing position. The
position will become available in July 2000.


Contacts: Professor Adrienne R. Hardham: Telephone 61 2 6249 4168,
FAX 61 2 6249 4331, Email Hardham-at-RSBS. ANU.edu.au.
Professor Bruce Walmsley: Telephone 61 2 6249 2039, FAX 61 2 6249 2687,
Email Bruce.Walmsley-at-ANU.edu.au.


Selection criteria are available from Susan Toscan. Telephone 61 2 6249
4752; FAX 61 2 6249 4891; Email Susan.Toscan-at-ANU.edu.au


Closing date: 12 June 2000

Applications addressing the selection criteria should be submitted to Susan
Toscan, RSBS, ANU, Canberra 2601 ACT quoting reference number and including
curriculum vitae, list of publications and names and addresses of at least
three referees.




From daemon Sun May 21 00:35:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 20 May 2000 12:29:07 +1000
Subject: RE: Our management has finally agreed to connect my electron microscopes to a recirculating water system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With that agreement you also have a few new problems to consider. I suggest
that:
In Australia you could not buy a large system like that as a supplied item.
Several smaller plants would be much too expensive and cumbersome.
Any imported system will result in problems with fittings and parts

I expect that it would be best (been there, done that a couple of times) to
design your own large system with a bit of spare capacity.

Beer chillers as used in hotels are most suitable. You want at least two large
chillers, so if one fails you still could run more than half of the equipment).
Calculate total heater element wattage with some spare capacity.

Diffusion pumps are designed to work best at 16 degrees. At that temperature
the TEM column will condense water. Depending on ambient temperature, it might
be sufficient to use the dif pump return line to cool the column and so avoid
that problem. The chillers should be installed under an open shelter as they
generate a lot of heat. Chiller temperature cut-in and out requires a
thermostat each.

I suggest that a centrifugal pump of 1hp capacity will give enough pressure at
the relative small flow rates. Chose a very common pump make so you can install
a spare quickly without replacing fittings. Other pump types give more
pressure, but they are more expensive and generally less reliable.

12mm reinforced garden hoses with hose clamps to and from the instruments make
installation easy, pretty secure and cause minimal pressure drop.

Required is a SS tank (insulated) or a fiberglass lined tank to act as a heat
sink. 100 liters would be a reasonable minimum capacity.

A good inline filter is required and a bypass, so the system can run during
filter change.

If a mains water line is maintained, it is very important to have the returning
water going to a drain whenever mains water is in use. I have lived through
some horrid floods, which resulted from people opening mains but not the bypass
to the drain.

If you have the components any plumber can install the system in under a day.
The plumber would supply gate valves, temperature and pressure gauges etc.

Hans, you may fax your rough design to me, I'll be happy to make any
suggestions.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, May 19, 2000 10:50 AM, Hans Brinkies
[SMTP:HBrinkies-at-groupwise.swin.edu.au] wrote:
}
} Our management has finally agreed to connect my electron microscopes to
} a recirculating water system.
}
} I need some ideas what type of systems are on the market and the
} Australian agent for these units. We need to attach 2 SEMs, 1 TEM plus 1
} coating unit to the new reciculating line.
}
} Thank you
}
} Hans Brinkies
} Senior Lecturer
} Swinburne, University of Technology
} School of Engineering and Science
} P.O.Box 218 - Hawthorn - Vic -3122 - Australia
} Phone: +61 3 9214 8657
} Fax: +61 3 9214 8264
} Email: Hbrinkies-at-swin.edu.au
}



From daemon Fri May 19 22:08:24 2000



From: Edward Hirsch :      edhirsch-at-att.net
Date: Fri, 19 May 2000 22:15:31 -0500
Subject: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
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John,
The solutions that the colloidal silica are in have a ph of 8.5 to 9.8
depending on the formulation. I suggest you try our 0.05 micron water based
polycrystalline diamond suspension. Allied High Tech
http://www.alliedhightech.com sells this product. I would also suggest our
Final A cloth for this polishing step. If you would like samples of either
of these products please let me know and I will be happy to send it to you.

If you need further technical assistance please contact me off-line and I
will be happy to help or you may contact our main office at (800)675-1118
located in CA.

I hope this helps.
Ed
Please note, I have a financial interest in providing you with these
products and other sample preparation equipment and consumable items.

*************************************************
Edward A. Hirsch
Product Application Specialist
Allied High Tech Products
2376 East Pacifica Place
Rancho Dominguez, CA 90220
ph: (919) 846-9628
vm:(800)675-1118 x245
fx: (310)762-6808
http://www.alliedhightech.com

Equipment and Consumables for Metallurgical Sample Preparation
*************************************************

-----Original Message-----
} From: john david whitaker [mailto:jwhitake-at-u.washington.edu]
Sent: Friday, May 19, 2000 4:01 PM
To: Microscopy Lister Server


I'm looking for information regarding final polishing
solutions for preparing metallic cross-section samples
for TEM analysis.

I've looked into colloidal silica
suspensions such as Ludox and Syton, and am concerned
that their alkilinity may chemically etch my samples.
I'm analyzing different compositions of NiFe
on copper substrates, so the specimens are prone to
preferential etching of different phases.

Does anyone have any experience with this?

Any input would be appreciated.

John





From daemon Sun May 21 02:25:56 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Sun, 21 May 2000 01:06:53 -0500
Subject: Pt shadowing of ultrasmall gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Would Pt shadowing of a protein-DNA complex interfere with an
ultrasmall
gold label on the protein?
Rosemary




From daemon Sun May 21 02:25:56 2000



From: ComCryLab1-at-aol.com
Date: Sun, 21 May 2000 01:11:08 -0500
Subject: EM: Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friends,
Can anyone give me advice regarding the dismantling and
the packing for shipment of a Philips 201 TEM? I need to
pay particular attention to not disturbing the alignment.
I plan to move the TEM from Univ. of Delaware to Naples, Florida
in an enclosed U-Haul Trailer. Any help or suggestions
will be most welcome.
If anyone has hands-on experience in doing this sort of thing
and is willing to give me a hand at U-Del, please contact me
and name your price. I'll gladly pay for the assistance.
Best regards,
Mike Urbanik
www.crystalguru.com




From daemon Mon May 22 00:40:17 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 21 May 2000 18:38:27 +1000
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I find what you are trying to do interesting, but there are things that may
never work and this may be one.

We had it confirmed by Michael Bode that good digital system "see "single
photons - so that is not likely to improve much. Film too registers single
electrons. We were told that in digital 1 electron can expose one pixel. On
film one electron initiates the exposure of a silver halide and subsequent
electrons in a near identical location increase the size of the grain.

Low noise (cooled cameras) hopefully do not add their own noise, but they
cannot make up for lack of information in the image.

My case (below) was that in high resolution TEM at least, less exposed images
will be inferior because there are simply not enough electrons to produce a
good image. A more sensitive digital system uses fewer electrons and so makes
matters worse.

In dark field EM we are using the beam indirectly and such images too suffer
particularly from electron noise. Here too the use of a digital system would
only make this worse. Furthermore, the slower 4489 film would be better than
SO-163.

If you don't like long exposures (4 to 8 seconds I found a practical limit),
you cannot ignore the reality of electron noise and hope to correct that with
yet fewer electrons, albeit processed in a "noise-free" digital system.
I think that the answer is more electrons, i.e. a field emission source or a
slower digital camera.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, May 19, 2000 3:04 PM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Jim hello
}
} I was talking about very special EM case: dark-field TEM. At this point we
} are talking about a few electrons per square angstrom, even less. In high
} resolution EM crystallography people have deal with 0.6 e/A2. At such
} conditions, high sensitivity and linearity of the modern digital cameras
} may be a plus. Talking about long exposures --what about drift? I never
} had good pictures at x100K with exposure longer that a few seconds. I
} don't understand your point about noise. In case of digital camera the
} noise is a function of camera. Current cameras has a very low level of
} noise (they uses cooling, etc) and we have to pay for that astronomical
} price. This is a life. I am not friendly with TEM cameras, but I do know
} that in the light microscopy people count individual photons using CCD
} cameras. In TEM camera we have phosphorous screen as a source of image and
} my questions to Mickhael were addressed mostly to the problem how
} effectively (and correctly) information is traveled thought that funny
} screen. The Mickhael's answer is that screen does not affect dynamic range
} and sensitivity is much higher than on convention films. Am I correct,
} Mickhaels?
}
} Sergey
}
} } Date: Thu, 18 May 2000 14:15:02 +1000
} } From: jim {jim-at-proscitech.com.au}
} } Subject: RE: new developments in imaging systems?
} } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} } "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} } Organization: ProSciTech
} } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } If sensitivity was ten times higher, one would in some cases save some beam
} } damage and in most cases suffer excessive electron noise.
} } As a rough guide, electron noise becomes apparent at 30x enlargement,
} rarely a
} } problem. Magnification is a linear function and electron density relates
} to an
} } area, but clearly very short exposure images would be much noisier and
} could
} } not be enlarged nearly as much. When not enough electrons form an image it
} } appears grainy, which makes it unsuitable for further enlarging. Photo
} } enlarging utilises higher depths-of-field and without this, very high
} power TEM
} } is much, much harder.
} } By nature, slower emulsions have finer grain and higher resolution and
} } contrast. It is fortuitous that these desirable characteristics run in
} tandem
} } with relatively long exposures, so the image is formed by more electrons.
} } TEM films rate about 6 ISO, so X-ray film at about ISI 3000 is about a 10x
} } faster than TEM films. Unfortunately its rather grainy, but if the
} exposure was
} } well adjusted, chances are that electron noise would be more bothersome than
} }
} } the film's grain.
} } Digital is inevitable and already fairly common, but some aces remain with
} } conventional film.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Thursday, May 18, 2000 5:06 AM, Sergey Ryazantsev
} [SMTP:sryazant-at-ucla.edu]
} } wrote:
} } }
} } }
} } } Thank you Mickhael.
} } }
} } } I will wait for info about sensitivity. I think, for EM in particular,
} } } sensitivity is very important parameter of the system. I am surprised
} } } that
} } } manufacturers don't have data on this matter. If sensitivity, say 10
} } } times
} } } higher than SO-163 film - it may be a great reason to switch from film to
} } } the CCD. The major disadvantage of the CCD cameras for TEM is their price
} } } in my point of view.
} } } Thanks for your respond.
} } }
} } } Sergey
} } }
} } }
} } } } Date: Wed, 17 May 2000 11:34:05 -0600
} } } } From: Michael Bode {mb-at-Soft-Imaging.com}
} } } } Subject: RE: new developments in imaging systems?
} } } } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} } } } X-Mailer: Internet Mail Service (5.0.1457.3)
} } } }
} } } } Sergey,
} } } }
} } } } I will try to find out what we have regarding the relative
} } } } sensitivities. One thing that I can say now is, that we acquire images
} } } } with a 50 or 100 msec exposure of the digital camera when the film
} } } } requires an exposure of about 2 seconds. This would mean a 20 to 40
} } } } times better sensitivity of the CCD camera. But to answer your question
} } } } in more detail would require to compare also the resolution of film and
} } } } camera and that is where it becomes very difficult, as it is not easy to
} } } } determine the resolution of film in terms of spatial resolution and
} } } } dynamic range, as both are interwoven. I will try to find some answers
} } } } for you.
} } } }
} } } } Regarding the linearity: As the CCDs simply count Photons, they have
} } } } almost perfect linearity over their complete dynamic range. Even more so
} } } } for TEMs, where all Photons have the same energy and the quantum
} } } } efficiency does not change from photon to photon. The Phosphor is a part
} } } } of a system that could theoretically introduce some non-linearities.
} } } } However, I did measure the linearity of some TEM camera systems a few
} } } } years back and did not find any significant deviations from linearity.
} } } }
} } } } Michael
} } } }
} } } } Michael Bode, Ph.D.
} } } } Soft Imaging System Corp.
} } } } 1675 Carr St., #105N
} } } } Lakewood, CO 80215
} } } } ===================================
} } } } phone: (888) FIND SIS
} } } } (303) 234-9270
} } } } fax: (303) 234-9271
} } } } email: mailto:info-at-soft-imaging.com
} } } } web: http://www.soft-imaging.com
} } } } ===================================
} } } }
} } } }
} } } }
} } } } -----Original Message-----
} } } } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} } } } Sent: Wednesday, May 17, 2000 1:29 AM
} } } } To: Michael Bode
} } } } Subject: RE: new developments in imaging systems?
} } } }
} } } }
} } } } Mickhael hello
} } } }
} } } } I have question for you. I am thinking about adding CCD camera to my
} } } } JEM1200EX. The information I gathered from Internet is not so
} } } } optimistic.
} } } } The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} } } } a
} } } } max as I understand. The price for cheaper camera is about 20-30 K$ -
} } } } much
} } } } more that I expect to spend for "film" process. There are two things
} } } } may
} } } } attract me to the modern CCD camera: dynamic range and sensitivity. I
} } } } am
} } } } pretty sure that dynamic range for CCD itself is a few orders better
} } } } than
} } } } any film available. But what about phosphorus screen? Does it reduce
} } } } dynamic range for the EM images? How dramatic? This is my first
} } } } question.
} } } } The next question is: could you tell me something about sensitivity of
} } } } the
} } } } modern CCD cameras used in EM? I am using dark field imaging at x80K
} } } } magnification and the exposure time for SO-163 (non deluded D-19, 7 min,
} } } } 20oC) is about 2 sec. I called GATAN, but they did not say anything
} } } } useful.
} } } } Could you provide some comparison of your side-mount camera with
} } } } sensitivity of the SO-163 film at condition I mentioned? I will greatly
} } } } appreciate any information in this matter. Thanks. Sergey.
} } } }
} } } }
} } } } } Date: Fri, 12 May 2000 13:46:01 -0600
} } } } } From: Michael Bode {mb-at-Soft-Imaging.com}
} } } } } Subject: RE: new developments in imaging systems?
} } } } } To: "'Microscopy-at-MSA.Microscopy.Com'"
} } } } {Microscopy-at-sparc5.microscopy.com}
} } } } } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } } } } X-Mailer: Internet Mail Service (5.0.1457.3)
} } } } }
} } } } } -----------------------------------------------------------------------
} } } } -
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } }
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } -----------------------------------------------------------------------
} } } } .
} } } } }
} } } } }
} } } } } Margaret,
} } } } }
} } } } } As a former user and current vendor of such systems as you are
} } } } inquiring
} } } } } about I can try to provide a bit of information regarding camera
} } } } } improvements:
} } } } }
} } } } } There have been a number of improvements, but I am not sure what you
} } } } are
} } } } } comparing the latest cameras against. Cameras are now usually cooled
} } } } and
} } } } } provide 12 bits per pixel, the number of pixels has gone up a bit (but
} } } } } not much in general), and cameras read out faster than they used to (up
} } } } } to 20 fps and more). I think all cameras now use a line transfer
} } } } } mechanism, which makes shutters obsolete.
} } } } } On the software side, real-time FFT and real-time shading correction
} } } } can
} } } } } be done now due to faster computers without special processing boards,
} } } } } and there have been other software developments that make using the
} } } } } cameras and computers easier.
} } } } } Other changes that affect the usability of cameras is the use of
} } } } } pneumatics to insert and retract the phosphors, higher frame rates for
} } } } } live viewing with the camera, etc.
} } } } }
} } } } } If you have questions, please give me a call, drop me an email, or go
} } } } to
} } } } } our web site.
} } } } }
} } } } } Michael
} } } } }
} } } } }
} } } } } Michael Bode, Ph.D.
} } } } } Soft Imaging System Corp.
} } } } } 1675 Carr St., #105N
} } } } } Lakewood, CO 80215
} } } } } ===================================
} } } } } phone: (888) FIND SIS
} } } } } (303) 234-9270
} } } } } fax: (303) 234-9271
} } } } } email: mailto:info-at-soft-imaging.com
} } } } } web: http://www.soft-imaging.com
} } } } } ===================================
} } } } }
} } } } }
} } } } }
} } } } } -----Original Message-----
} } } } } } From: Margaret Dienelt [mailto:brannign-at-asrr.arsusda.gov]
} } } } } Sent: Thursday, May 11, 2000 12:43 PM
} } } } } To: Microscopy-at-sparc5.microscopy.com
} } } } } Subject: TEM: new developments in imaging systems?
} } } } }
} } } } }
} } } } } -----------------------------------------------------------------------
} } } } -
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } }
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } -----------------------------------------------------------------------
} } } } .
} } } } }
} } } } }
} } } } } Hi,
} } } } }
} } } } } Year after year I hopefully gather information about digital imaging
} } } } } systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
} } } } } money. This year it looks like it might really happen but I have not
} } } } } kept up with innovations in the field and am wondering the following:
} } } } }
} } } } } 1. Anything new in the last two years -- especially in terms of
} } } } } cameras? I'm most familiar with the Gatan and AMT systems but their
} } } } } web sites don't reflect much in the way of changes over a year ago.
} } } } } 2. With more and more microscopists finally getting their systems --
} } } } } I'd love to get feedback.
} } } } }
} } } } } Thanks,
} } } } } Margaret
} } } } }
} } } } } P.S. Would welcome contacts from vendors.
} } } } }
} } } } } --
} } } } } Margaret Dienelt
} } } } }
} } } } } Plant Pathologist
} } } } } Electron Microscopy Lab
} } } } }
} } } } } Floral and Nursery Plants Research Unit
} } } } } U.S. National Arboretum/Agricultural Research Service/USDA
} } } } }
} } } } } B. 010A, Rm. 238, BARC-W
} } } } } 10300 Baltimore Avenue
} } } } } Beltsville MD. 20705 USA
} } } } }
} } } } } (301) 504-6097
} } } } } Fax: (301) 504-5096
} } } } }
} } } } }
} } } } _____________________________________
} } } }
} } } } Sergey Ryazantsev Ph. D.
} } } } Electron Microscopy
} } } } UCLA School of Medicine
} } } } Department of Biological Chemistry
} } } } Box 951737
} } } } Los Angeles, CA 90095-1737
} } } }
} } } } Phone: (310) 825-1144
} } } } Pager: (310) 845-0248
} } } } FAX (departmental): (310) 206-5272
} } } } mailto:sryazant-at-ucla.edu
} } } } http://www.bol.ucla.edu/~sryazant
} } } }
} } } }
} } } _____________________________________
} } }
} } } Sergey Ryazantsev Ph. D.
} } } Electron Microscopy
} } } UCLA School of Medicine
} } } Department of Biological Chemistry
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } Phone: (310) 825-1144
} } } Pager: (310) 845-0248
} } } FAX (departmental): (310) 206-5272
} } } mailto:sryazant-at-ucla.edu
} } } http://www.bol.ucla.edu/~sryazant
} } }
} } }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}



From daemon Mon May 22 00:40:19 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Mon, 22 May 2000 01:10:41 +0400
Subject: Re: BSE resolution

Contents Retrieved from Microscopy Listserver Archives
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Dear Malc!
Some years ago I got the resolution 0.1 in the middle of periodic
table (around Z=28) on standard 733 BSE.
But for this purpose it is necessary to balance additionally the
preamplifier to remove completely the rest of topo signal, which
becomes comparable by value with a very low signal of differential
compo. There are no means in the preamplifier for this additional
balancing, therefore I have soldered the additional variable resistor
in one of shoulders of a differential amplifier in the preamplifier.
Frankly speaking I have forgotten the details already, but I can
restore them, if you wait about two weeks. But basically it is very
simple.
Regards.
Victor Sidorenko, ANTRON Co. Ltd., scientific service, Moscow, Russia.

-----閛”Š’”Œ –””‡Œ’Œ-----
ë˜: Dr Malcolm Roberts {malc-at-rock.ru.ac.za}
攓—: Microprobe discussion group {Microscopy-at-sparc5.microscopy.com}
€ˆ˜ˆ: 19 “ˆØ 2000 ‹. 16:13
񌓈: BSE resolution


} ---------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America



From daemon Mon May 22 00:40:19 2000



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Sun, 21 May 2000 17:42:46 -0400
Subject: RE: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

Polishing suspensions that does not interact chemically with your samples
include diamond suspensions (available down to 0.1µm at least) and alumina
suspensions (down to 0.05µm). Many vendors have information available on the
World-Wide-Web.

Cheers,
Paul
===================
Paul Baggethun
Alcoa Technical Center
Alcoa Center, PA 15069
USA
===================
+ 724 - 337-1760 (tel)
+ 724 - 337-2044 (fax)
===================


} ----------
} From: john david whitaker[SMTP:jwhitake-at-u.washington.edu]
} Sent: Friday, May 19, 2000 5:01 PM
} To: Microscopy Lister Server
} Subject: final polishing for metals (Cu, Ni, Fe, NiFe)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm looking for information regarding final polishing
} solutions for preparing metallic cross-section samples
} for TEM analysis.
}
} I've looked into colloidal silica
} suspensions such as Ludox and Syton, and am concerned
} that their alkilinity may chemically etch my samples.
} I'm analyzing different compositions of NiFe
} on copper substrates, so the specimens are prone to
} preferential etching of different phases.
}
} Does anyone have any experience with this?
}
} Any input would be appreciated.
}
} John
}
}



From daemon Mon May 22 00:40:24 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Sun, 21 May 2000 13:40:29 -1000 (HST)
Subject: Re: TEM: Help

Contents Retrieved from Microscopy Listserver Archives
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On Thu, 18 May 2000 ComCryLab1-at-aol.com-at-sparc5.microscopy.com wrote:

} Dear Friends,
} Can anyone give me advice regarding the dismantling and
} the packing for shipment of a Philips 201 TEM? I need to
} pay particular attention to not disturbing the alignment.
} I plan to move the TEM from Univ. of Delaware to Naples, Florida
} in an enclosed U-Haul Trailer. Any help or suggestions
} will be most welcome.

I will make sure Ron Veil, a very experienced independent EM service tech,
sees this and has a chance to reply. It was with his advice that we (my
hubby and I and its new owner) packed up and shipped a Philips 201. Like
you, we wanted to ship it intact, column on and all. We built a heavy
duty skid and added a strong base to which we bolted the instrument. Then
we wrapped it with whatever that plastic packing tape that is like Saran
Wrap is called, making sure to secure any part of the column we didn't
want to move, but avoiding putting any pressure on things, such as the
aperture drives. Wrap the bottom, wrap the column, wrap the column to the
bottom and back again, etc. Build a crate up around the instrument.
I think I remember putting a wood insert with a crescent cut out near the
top of, but not touching the column, but hubby thinks not. The idea is
NOT to let any shock to the crate get transferred to the column, so
perhaps we didn't. Add some packing material, such as old egg crate foam
from your bed (it needed replacing anyway) and whatever. Then, and this
was the fun part, buy that stuff that when you mix it with a catalyst,
produces huge volumes of foam that hardens in a few minutes. I think you
can also buy spray cans of similar stuff, but since hubby had this on hand
for other things, we had the bulk stuff. Fill the voids in the crate with
it. It will easily chip off later. Add a top, and away you go. The
scope made it in great shape.

I think we packed the rotary pump and a couple of other things
separately. Get it into the truck and TIE IT DOWN. I say this because an
SEM we once packed for shipping with the base and saran, but no closed
crate, made it from Hawaii to Ohio, then slid in the shippers truck in the
last mile and slammed into the driver's seat. The driver was OK, the ion
getter pump was bent, and the column needed a bit of work, but it could
have been worse. Duh.

I've also packed up a couple of Denton vcuum evaporators and a couple of
ultramicrotomes. The saran stuff and blow foam are a good way to
stabilize the instruments.

Good luck!

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon May 22 00:40:24 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sun, 21 May 2000 22:51:58 -0500
Subject: Re: TEM: Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: "Tina Carvalho" {tina-at-pbrc.hawaii.edu}
} On Thu, 18 May 2000 ComCryLab1-at-aol.com-at-sparc5.microscopy.com wrote:
}
} } Dear Friends,
} } Can anyone give me advice regarding the dismantling and
} } the packing for shipment of a Philips 201 TEM? I need to
} } pay particular attention to not disturbing the alignment.
} } I plan to move the TEM from Univ. of Delaware to Naples, Florida
} } in an enclosed U-Haul Trailer. Any help or suggestions
} } will be most welcome.
}
} I will make sure Ron Veil, a very experienced independent EM service tech,
} sees this and has a chance to reply. It was with his advice that we (my
} hubby and I and its new owner) packed up and shipped a Philips 201. Like
} you, we wanted to ship it intact, column on and all. We built a heavy
} duty skid and added a strong base to which we bolted the instrument. Then
} we wrapped it with whatever that plastic packing tape that is like Saran
} Wrap is called, making sure to secure any part of the column we didn't
} want to move, but avoiding putting any pressure on things, such as the
} aperture drives. Wrap the bottom, wrap the column, wrap the column to the
} bottom and back again, etc. Build a crate up around the instrument.
} I think I remember putting a wood insert with a crescent cut out near the
} top of, but not touching the column, but hubby thinks not. The idea is
} NOT to let any shock to the crate get transferred to the column, so
} perhaps we didn't. Add some packing material, such as old egg crate foam
} from your bed (it needed replacing anyway) and whatever. Then, and this
} was the fun part, buy that stuff that when you mix it with a catalyst,
} produces huge volumes of foam that hardens in a few minutes. I think you
} can also buy spray cans of similar stuff, but since hubby had this on hand
} for other things, we had the bulk stuff. Fill the voids in the crate with
} it. It will easily chip off later. Add a top, and away you go. The
} scope made it in great shape.
}
} I think we packed the rotary pump and a couple of other things
} separately. Get it into the truck and TIE IT DOWN. I say this because an
} SEM we once packed for shipping with the base and saran, but no closed
} crate, made it from Hawaii to Ohio, then slid in the shippers truck in the
} last mile and slammed into the driver's seat. The driver was OK, the ion
} getter pump was bent, and the column needed a bit of work, but it could
} have been worse. Duh.
}
} I've also packed up a couple of Denton vcuum evaporators and a couple of
} ultramicrotomes. The saran stuff and blow foam are a good way to
} stabilize the instruments.
}
Since you are using a U haul you don't have to worry about sides
and a top on the crate. Make sure it is tied down real well. Dry
wall screws through the crate and into the floor work well for
locking it down but make sure you have it braced with timbers
to the front and sides of the trailer in case you make a panic
stop.

Also make sure that the weight is centers in front of the trailer
axle if you use a trailer. Negitive weight on the trailer tounge
at the very least makes driving very interesting. You have no
Idea how fast a trailer can pass you while it is still tied on to the
truck. I have had the privilege of experiencing this and I can promise
you that you won't enjoy it:).

The foam in place stuff is great. Just cover the instrument in plastic
and foam away. If you can get foam between the instrument and the
Support the foam will dissipate most of those little shocks that get
things out of line.

Last of all make sure you understand what you insurance covers and
what it doesn't on moving equipment. You should be fine on liability but
the value of the contents is probably not covered. Rental truck companies
have contents insurance as an extra.

Good luck
Gordon

Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00







From daemon Mon May 22 10:19:51 2000



From: NIKONBIO-at-aol.com -at-oldsparc5.Microscopy.Com
Date: Mon, 22 May 2000 00:55:45 -0500
Subject: JOB ANNOUNCEMENT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

I would like to announce an exciting job opportunity to the Microscopy
community.


JOB ANNOUNCEMENT

POSITION TITLE: SR. SCIENTIST/ENGINEER LIGHT MICROSCOPY NIKON INC.
MELVILLE, NY
Posted: May 18, 2000

As seen in the 19 May issue of Science:
http://recruit.sciencemag.org/cgi/show/5469/5469x03207

Sr. Applications Scientist/Engineer
Bring Your Light Microscopy
Experience To The BioScience Division Of Nikon Inc.

Our world renowned company has an excellent opportunity for a professional
with extensive experience in light microscopy, specifically in advanced
BioScience technologies in our USA headquarters located in Melville, Long
Island , NY. Today, scientists at world-renowned biological institutions are
making tremendous advances in Cancer, AIDS, Alzheimer's, in-vitro
fertilization and other leading-edge research. Nikon is proud to be playing a
role in this enormously important work. Our ongoing commitment to optical
excellence and technological advancement is allowing researchers to view
objects never before seen by the human eye. We would like to invite you to
investigate the new opportunities and technologies available at Nikon Inc.
BioSciences.


JOB SUMMARY

Provide the principal conduit for technical information and feedback between
the end user/sales network and Nikon Factory engineers on: product
applications, product acceptance, competitive changes, required product
improvements, new technologies and methodologies that impact Nikon's present
and future business. Provide the sales force with technical assistance to
successfully satisfy customer's needs and complete the sale. Create and
conduct training curriculum on advanced microscopy and new technologies to
Nikon distribution and end users. Attend trade shows, workshops and national
dealer / sales meetings. Write technical and applications bulletins to end
users and distribution channels as well articles for publication in Bio
journals highlighting Nikon technology or applications.


Selected candidate will act as liaison for technical information and feedback
between the end user/sales network and Nikon factory engineers on all matters
pertaining to our BioScience technical product line. Applicants must have a
Ph.D. in BioSciences, BioEngineering or Physics with a specialty in optics,
or equivalent experience. Knowledge of advanced microscopy techniques and
optical principals including fluorescence applications, imaging and confocal
applications required. Excellent communications skills in English a must,
along with good writing and public speaking abilities. Excellent benefits and
compensation provided.


Mail or fax your resume, which MUST include salary requirements, to:
HR Department, Nikon, 1300 Walt Whitman Road, Melville, NY 11747. Fax:
631-547-4025.

Or send a digital cover letter and resume / CV in (plain text, MS Word, or
.pdf file) to biosales-at-nikonincmail.com

Check our website: www.nikonusa.com.

Also See add display in NY Times:
http://search.nytimes.com/classified/display/adverts/524986401/

Nikon is an Equal Opportunity Employer
=============================================================

Best Regards,

Stan Schwartz
Manager BioSciences Dept.
Nikon Inc.
1300 Walt Whitman Rd.
Melville, NY 11747
631-547-8500




From daemon Tue May 23 00:29:37 2000



From: lherault :      lherault-at-bu.edu
Date: Mon, 22 May 2000 02:56:09 -0400
Subject: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've had to coat some PLGA polymer samples and we think the coater may be
melting them. The samples have a rolled or beaded edge and large cracks. I
have jpeg images if anyone wants me to send them for a look. Are we
correct in our assessment or should we look elsewhere for the source of
cracks, shrinkage, beading around edges. These artifacts, BTW do not appear
when the uncoated samples are checked under a light microscope.

Thanks in advance.

Ron L
lherault-at-bu.edu



From daemon Mon May 22 10:19:52 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Mon, 22 May 2000 10:46:58 +0200
Subject: TEMalert - A pre-print anouncement system for TEM region.

Contents Retrieved from Microscopy Listserver Archives
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Dear friends,
I have just finished writing the programs for TEMAlert system(http://www6.ewebcity.com/temalert), which serves as a pre-print announcement system. Currently it serves only TEM-related region. Do you think this is very useful for all TEM users? The system was designed to be totally self-maintaining and everyone can post messages to announce his/her newly published (accepted) paper there.

I hope that TEMAlert will become a widespread blackboard in TEM region and tighten the relationship between electron microscopists allover the world.

I am sorry that the server is somewhat slow - because it is a free internet host. I will be very grateful if anyone of you can supply me a space (should support ASP).

With best regards,
Shu-You Li
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Please visit my new homepage - a personal website on transmission electron microscopy (TEM) - at http://syli.homepage.com at your convenience!
It contains
my current research work, my resume
TEM-related journals, instruction for authors
link to on-line EELS database, periodic table, physical constants
JOB list and RESUMEs (only for TEM region)
TEMAlert - a self-maintaining preprint announcement system
**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************






From daemon Mon May 22 10:19:53 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Mon, 22 May 2000 20:49:43 +1000
Subject: RE: Our management has finally agreed to connect my electron microscopes to a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans, Jim,

} In Australia you could not buy a large system like that as a supplied item.

Don't know about that...We've had excellent results from a locally made
system we've been using here now on the SEMs for several years. Service and
parts are readily available and it has heaps of spare capacity. Contact
Mark Blackford (mgb-at-postoffice.ansto.gov.au) for details on who to contact
about this system because they probably have a branch in Melbourne.

}
} Beer chillers as used in hotels are most suitable.

Certainly a mass produced item in Australia.



Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Mon May 22 10:19:56 2000



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Mon, 22 May 2000 09:55:45 -0400
Subject: video from single images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello,

I have a batch of images (~300) that I would like to present as a
QuickTime movie. Does anybody have experience how to do this? I am
using a Macintosh. Comments welcome. Thanks,

Edgar


________________
Dr. Edgar Voelkl
ORNL
Bldg 4515
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov



From daemon Mon May 22 10:19:57 2000



From: Holt, Scott :      Scott.Holt-at-buehler.com
Date: Mon, 22 May 2000 09:03:15 -0500
Subject: Final Polishing of Metals

Contents Retrieved from Microscopy Listserver Archives
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John,

As a metallographic equipment and consumables manufacturer, BUEHLERš offers
a number
of products which would work for your application. However, I would suggest
our
MASTERPREP* (Part No. 40-6377-032) product. This is a 0.05 micron alumina
suspension. What makes it unique is the fact that it is produced through
the seeded
gel process instead of by calcining. In the seeded gel process, the alumina
is
precipitated from a liquid phase. This results in a better controlled
particulate
size distribution, higher particulate density, and more consistent particle
geometry. We've found
this product to be superior to all of the other 0.05 micron alumina products
that we sell for
preparation of ductile metals.

I hope this helps. If you need further information, you can email me
privately, call me at the
numbers listed below, or contact our sales department for pricing.

Best regards,
Scott D. Holt
BUEHLERš , LTD.
PO Box 1
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546
or (800) 323-9330
www.buehler.com



From daemon Mon May 22 10:19:58 2000



From: Linda Barthel :      barthel-at-umich.edu
Date: Mon, 22 May 2000 10:44:08 -0400 (EDT)
Subject: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am also trying to figure out how to make a QuickTime video from a series
of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
in LSM format, which have been converted to Tiff. The number I am dealing
with is 64 images. Any help would be greatly appreciated
Linda Barthel
Research Associate II
Department of Cell and Developmental Biology
University of Michigan
barthel-at-umich.edu





From daemon Mon May 22 10:20:00 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Mon, 22 May 2000 16:48:59 +0200
Subject: Philips Xl30 ESEM applications workshop required

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

We have a client, here in South Africa, who has recently purchased a Philips
XL30 ESEM LaB6 with a cryo system, hot stage and CL detectors.
We would like to now run a workshop, here on this system in South Africa, on
the applications of ESEM.
This system is a national facility and would therefore like to introduce the
local EM users to the exciting world of ESEM.

We realise that there are a few friends of ours in Australia who would be
ideal, but then we have various contacts in England and the USA too. In this
way we feel we should get a chance at the best choice of getting a really
exciting workshop set up or possibly a series of workshops.
We expect to keep the delegates riveted for at least 4 days of workshop. The
idea of the workshop is to show as many applications for ESEM as possible.
Then to specialise in some of the biological areas, as this would be some of
the more regular users for this system.
Those who could assist should please contact us and we will pass your
information on to the client. Please indicate your availability, field of
interest and the, always important, costs involved.

Thanks for your time.

Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
Fax + 27 (0) 11 476 7290
anaspec-at-icon.co.za
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com



From daemon Mon May 22 10:20:01 2000



From: Jean Dille :      jdille-at-ulb.ac.be
Date: Mon, 22 May 2000 17:05:00 +0200
Subject: cross sections of layered materials

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

We have a sample of 6 micrometers Ni-Zn-P electroless plated onto 1 mm
thick Al
and we would like to prepare cross sections for TEM examination.
It looks very difficult to use the tripod polishing technique for metals.So,we
are thinking at an electropolishing procedure with our double-jet Tenupol
apparatus.
This would be done on slices cutted from a sample initially thicked up to 3mm
by electroplating.But how to avoid differential material removal problems?
Which material for plating?Which electrolyte for electropolishing?Other
method?
Does anyone have suggestions for us?

Thanks in advance for your help.

Jean Dille

Materials Science and Electrochemistry
Free University of Brussels CP 194/3
Avenue F.Roosevelt 50
1050 BRUSSELS
BELGIUM
tel:32-2-6502723
fax:32-2-6502786
e-mail: jdille-at-ulb.ac.be




From daemon Tue May 23 00:28:44 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 22 May 2000 09:38:16 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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Yes, Jim, this is absolutely correct.

The results are determined by the largest source of noise, be it the
initial electron statistics or subsequent noise introduced by
conversions or recording techniques. In this case (dark field imaging),
the largest source for noise is probably the statistical nature of the
electron beam. The lower you go in exposure (i.e., the fewer electrons
you record per pixel), the higher the relative noise. And there is no
way to get around this either by film or digital camera. The only way to
improve the noise is to go to more electrons, i.e., longer exposure or
brighter beam.

If the sample is so sensitive that even a dark field exposure damages
the sample, there is probably not much you can do.

If the problem is drift, a digital system can help you: Instead of 1
exposure at, for example, 30 seconds, acquire 3 exposures at 10 seconds.
Each one of them will be very noisy, but one can add them to get the
same noise figure as a 30 sec exposure. And the 10 sec exposure of each
one cuts down on drift. All you need to do is to align the 3 images.

I used to take dark field images with a digital camera and (of course) I
loved it. It allowed me to acquire the images and immediately see them.
I did not have to wait hours to develop the negatives and then find out
the exposure was not long enough (or too long).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Sunday, May 21, 2000 2:38 AM
To: 'Sergey Ryazantsev'; Microscopy-at-sparc5.microscopy.com


I find what you are trying to do interesting, but there are things that
may
never work and this may be one.

We had it confirmed by Michael Bode that good digital system "see
"single
photons - so that is not likely to improve much. Film too registers
single
electrons. We were told that in digital 1 electron can expose one pixel.
On
film one electron initiates the exposure of a silver halide and
subsequent
electrons in a near identical location increase the size of the grain.

Low noise (cooled cameras) hopefully do not add their own noise, but
they
cannot make up for lack of information in the image.

My case (below) was that in high resolution TEM at least, less exposed
images
will be inferior because there are simply not enough electrons to
produce a
good image. A more sensitive digital system uses fewer electrons and so
makes
matters worse.

In dark field EM we are using the beam indirectly and such images too
suffer
particularly from electron noise. Here too the use of a digital system
would
only make this worse. Furthermore, the slower 4489 film would be better
than
SO-163.

If you don't like long exposures (4 to 8 seconds I found a practical
limit),
you cannot ignore the reality of electron noise and hope to correct that
with
yet fewer electrons, albeit processed in a "noise-free" digital system.
I think that the answer is more electrons, i.e. a field emission source
or a
slower digital camera.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, May 19, 2000 3:04 PM, Sergey Ryazantsev
[SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Jim hello
}
} I was talking about very special EM case: dark-field TEM. At this
point we
} are talking about a few electrons per square angstrom, even less. In
high
} resolution EM crystallography people have deal with 0.6 e/A2. At such
} conditions, high sensitivity and linearity of the modern digital
cameras
} may be a plus. Talking about long exposures --what about drift? I
never
} had good pictures at x100K with exposure longer that a few seconds. I
} don't understand your point about noise. In case of digital camera
the
} noise is a function of camera. Current cameras has a very low level
of
} noise (they uses cooling, etc) and we have to pay for that
astronomical
} price. This is a life. I am not friendly with TEM cameras, but I do
know
} that in the light microscopy people count individual photons using CCD
} cameras. In TEM camera we have phosphorous screen as a source of
image and
} my questions to Mickhael were addressed mostly to the problem how
} effectively (and correctly) information is traveled thought that funny
} screen. The Mickhael's answer is that screen does not affect dynamic
range
} and sensitivity is much higher than on convention films. Am I
correct,
} Mickhaels?
}
} Sergey
}
} } Date: Thu, 18 May 2000 14:15:02 +1000
} } From: jim {jim-at-proscitech.com.au}
} } Subject: RE: new developments in imaging systems?
} } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} } "Microscopy-at-sparc5.microscopy.com"
{Microscopy-at-sparc5.microscopy.com}
} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} } Organization: ProSciTech
} } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
} }
}
} -----------------------------------------------------------------------
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} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
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} -----------------------------------------------------------------------



From daemon Tue May 23 00:28:45 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Mon, 22 May 2000 09:36:41 -0700
Subject: Treated coverslips

Contents Retrieved from Microscopy Listserver Archives
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My thanks to all those who responded to my question about treating
coverslips to make cells stick to them better. I have used one or two of
these in the past, but my colleague wanted to try some different approaches.
Thanks again.


Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu



From daemon Tue May 23 00:28:46 2000



From: Simon C. Watkins :      swatkins+-at-pitt.edu
Date: Mon, 22 May 2000 12:51:15 -0400
Subject: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
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Linda, if you get quicktime pro (its an upgrade to regular quicktime, costs
about $30 from Apple) you can import a numbered series of tiffs directly
into quicktime. which then can be made into a movie
Simon


-----Original Message-----
} From: Linda Barthel [mailto:barthel-at-umich.edu]
Sent: Monday, May 22, 2000 10:44 AM
To: Microscopy listserver



I am also trying to figure out how to make a QuickTime video from a series
of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
in LSM format, which have been converted to Tiff. The number I am dealing
with is 64 images. Any help would be greatly appreciated
Linda Barthel
Research Associate II
Department of Cell and Developmental Biology
University of Michigan
barthel-at-umich.edu







From daemon Tue May 23 00:28:45 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 22 May 2000 12:55:22 -0400
Subject: old FF machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a functional, but old, Balzers Freeze Fracture machine. It is
headed for the scrap pile unless someone out there would like to have
it. I will explore the possibilities if anyone shows an interest.

Greg
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Tue May 23 00:28:46 2000



From: john david whitaker :      jwhitake-at-u.washington.edu
Date: Mon, 22 May 2000 09:57:51 -0700 (PDT)
Subject: RE: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed,

Thanks for the input. I was hesitant to use diamond abrasives
on my samples due to previous advice. I was told that due to
the hardness of diamond, it tends to embed itself in soft
metals such as copper, which results in "smearing" more than
polishing. Do you have any input regarding the smearing issue?

John

On Fri, 19 May 2000, Edward Hirsch wrote:

} John,
} The solutions that the colloidal silica are in have a ph of 8.5 to 9.8
} depending on the formulation. I suggest you try our 0.05 micron water based
} polycrystalline diamond suspension. Allied High Tech
} http://www.alliedhightech.com sells this product. I would also suggest our
} Final A cloth for this polishing step. If you would like samples of either
} of these products please let me know and I will be happy to send it to you.
}
} If you need further technical assistance please contact me off-line and I
} will be happy to help or you may contact our main office at (800)675-1118
} located in CA.
}
} I hope this helps.
} Ed
} Please note, I have a financial interest in providing you with these
} products and other sample preparation equipment and consumable items.
}
} *************************************************
} Edward A. Hirsch
} Product Application Specialist
} Allied High Tech Products
} 2376 East Pacifica Place
} Rancho Dominguez, CA 90220
} ph: (919) 846-9628
} vm:(800)675-1118 x245
} fx: (310)762-6808
} http://www.alliedhightech.com
}
} Equipment and Consumables for Metallurgical Sample Preparation
} *************************************************
}
} -----Original Message-----
} From: john david whitaker [mailto:jwhitake-at-u.washington.edu]
} Sent: Friday, May 19, 2000 4:01 PM
} To: Microscopy Lister Server
} Subject: final polishing for metals (Cu, Ni, Fe, NiFe)
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm looking for information regarding final polishing
} solutions for preparing metallic cross-section samples
} for TEM analysis.
}
} I've looked into colloidal silica
} suspensions such as Ludox and Syton, and am concerned
} that their alkilinity may chemically etch my samples.
} I'm analyzing different compositions of NiFe
} on copper substrates, so the specimens are prone to
} preferential etching of different phases.
}
} Does anyone have any experience with this?
}
} Any input would be appreciated.
}
} John
}
}
}
}



From daemon Tue May 23 00:28:57 2000



From: Kevin W. Eliceiri :      eliceiri-at-facstaff.wisc.edu
Date: Mon, 22 May 2000 12:04:44 -0500
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Our software, 4D turnaround will make quicktime movies out of image
stacks that are in biorad format, pics, or tiff. Please see our
website for more details and to download:
http://www.loci.wisc.edu/4d/native/4d.html

Currently we have only a mac version available, however we will be
releasing a java version next month that works cross platform.

Best regards,
kevin

Kevin W. Eliceiri
Project Director
Laboratory for Optical and Computational Instrumentation
http://www.loci.wisc.edu
159 Animal Sciences
1675 Observatory Dr.
Madison, WI 53706
608-263-6288 voice
608-265-4076 fax



From daemon Tue May 23 00:28:59 2000



From: Kuenzler Ruediger :      kuenzler-at-dibis.de
Date: Mon, 22 May 2000 19:15:17 +0200
Subject: Re: TEM: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Margaret,

A view days ago I found your message in my mailbox.
Regarding your interest about digital imaging systems I can provide you some
information on our imaging plate system for TEM.

DIBIS Imaging Plate Technology is adapted for TEMs from, FEI/Philips,
LEO/Zeiss, JEOL and HITACHI. The Imaging Plate (81 x 100 mm) is inserted
into the sheet film cameras of the various TEMs and is directly exposed by
electrons.
After that the imaging plate reader creates digital images directly from the
plates without involving chemical processing thus providing extraordinary
image quality with 3600 x 3200 pixel at 25 µm and 16 or 20 bit dynamic range
with true linearity.
The high pixel count supports printouts in real photographic quality, also
on larger formats as you are used to from photographic film.
One Instrument in your lab will serve all your TEMs with highest quality
digital imaging technology, no matter what type and manufacturer.
The high performance, resolution, sensitivity and dynamic range makes
Imaging Plate Technology first choice for life science and material sciences
imaging, low dose applications and high dynamic diffraction patterns.

So, DIBIS introduces MICRON Digital Imaging Plate Technology as the
alternative to overcome the limits of CCD technology for TEM.

For more details visit our homepage.


R. Kuenzler
----------------------------------
DIBIS
Digital Biomedical Imaging Systems AG
Gewerbestra§e 11; D-75217 Birkenfeld
Tel.: +49 (0)7082 940639
Fax : +49 (0)7082 940076
E-Mail: contact-at-dibis.de
E-Mail: kuenzler-at-dibis.de
Internet: http://www.dibis.de





Margaret Dienelt schrieb:

Hi,

}
} Year after year I hopefully gather information about digital imaging
} systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
} money. This year it looks like it might really happen but I have not
} kept up with innovations in the field and am wondering the following:
}
} 1. Anything new in the last two years -- especially in terms of
} cameras? I'm most familiar with the Gatan and AMT systems but their
} web sites don't reflect much in the way of changes over a year ago.
} 2. With more and more microscopists finally getting their systems --
} I'd love to get feedback.
}
} Thanks,
} Margaret
}
} P.S. Would welcome contacts from vendors.
}
} --
} Margaret Dienelt
}
} Plant Pathologist
} Electron Microscopy Lab
}
} Floral and Nursery Plants Research Unit
} U.S. National Arboretum/Agricultural Research Service/USDA
}
} B. 010A, Rm. 238, BARC-W
} 10300 Baltimore Avenue
} Beltsville MD. 20705 USA
}
} (301) 504-6097
} Fax: (301) 504-5096

--





From daemon Tue May 23 00:29:35 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 22 May 2000 11:10:11 -0700 (PDT)
Subject: RE: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In addition to QuickTime Pro,

GraphicConverter will convert a folder of numbered tiffs into QT. Just
be aware that after choosing the folder with your tiffs, and clicking
on Convert, the next window has an easily overlooked choice at its top
to save the files as ONE movie. Forget this selection and you will
have converted your 300 tiffs into 300 moov files. GraphicConverter has
many options for compression and output size.

NIH Image can open a folder of numbered tiffs with the 'Open All' option.
Use the 'Windows to Stack' command then save as QT. Image will place your
tiffs into the stack in the order in which they were opened. So if your
filenames don't end with an incrementing number you could open them
manually in the desired order.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

*********************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
*********************************************************************




From daemon Tue May 23 00:29:06 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Mon, 22 May 2000 13:14:00 -0500
Subject: Re:video from single images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am unsure what versions are available for the Mac, but I have made videos
using ULEAD video editing software for the PC. TIFF images can be imported
and
displayed for "N" number of frames. Special effects (like a wipe or fade)
can
be added between sets fo (still) frame sets.

Do beware this process can tax the resources of most PCs. If the video is
uncompressed, the data rate can be 10+ MB/sec. I use a Matrox video card
which has MJPEG hardware compression. At full VHS resolution and 15
frames/sec
(not 29.9) the data rate is below 2 MB/sec. With MJPEG, I can get about 10
minutes of "fair" quality video on one CD-R (650 MB). Mpeg compression can
make a smaller file, but I havent tried a compression program I like -
Lousy
quality - Jumps, etc. I haven't tried the MPEG program from Xing Tech.
which is
rated better thatn the sharewares I have tried.

Another issue is the conversion time using software mpeg convertors is that
10
minutes of MJPEG video to mpeg on my PC (350 MHz/256 Meg Ram) takes well
over an
hour.

Woody White



From daemon Tue May 23 00:29:36 2000



From: Tom Isabell :      tc_isabell-at-fischione.com
Date: Mon, 22 May 2000 14:17:59 -0400
Subject: Job Listing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


E.A. Fischione Instruments, Inc., a rapidly growing company specializing in the development and manufacture of TEM specimen preparation instrumentation, is seeking a highly motivated individual for the position of a Application Scientist.
The candidate's responsibilities will include:

* Obtain and prepare TEM specimens of customer's material for sales
purposes.
* Analyze customers' specimens in Fischione's TEM.
* Library research to support design and application work activities.
* Answer customers' questions regarding use of Fischione products.
* Instrument training (when required).
* Provide technical support at major tradeshows.
* Give product demonstrations.
* Collaborate with potential customers on experiments.
* Write applications notes.
* Give scientific presentations.
* Conduct short courses and workshops on specimen preparation and
other Fischione related technology.
* Write refereed journal articles for publication.
* Generate information for Website.
* Revise instrument instruction manuals.
* Evaluate emerging microscopy related technologies and market
opportunities.
* Convey market opportunities to Fischione management.
* Obtain input from customers on possible new products and on
improvements to existing products.
* Work with the product design team on new product developments.
* Provide design support from the microscopist's standpoint.
* Prototype and test both existing product improvements and new products.
* Procure needed instrumentation to get specimen preparation facility
running appropriately.
* Optimize Fischione's TEM laboratory.
* Work with architects to design new EM facility when building expansion
occurs.
* Travel approximately 10%-20%.

The candidate should have a Ph.D in Material Science, Engineering, or Physics with a specialization in Electron Microscopy.

Salary is commensurate with experience.

E.A. Fischione Instruments, Inc. is an equal opportunity employer. Please send your resume and salary requirements to:

Human Resources Director
E.A. Fischione Instruments, Inc.
9003 Corporate Circle Export, PA 15632
Phone (724) 325-5444 FAX (724) 325-5443
E-mail: info-at-fischione.com



From daemon Tue May 23 00:29:36 2000



From: Anda Cornea :      corneaan-at-ohsu.edu
Date: Mon, 22 May 2000 11:33:16 -0700
Subject: Axiocam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!

We are considering the purchase of an Axiocam and would appreciate comments from other users.

Thanks!

Anda Cornea, Ph.D.
Oregon Regional Primate Research Center
505 NW 185th Avenue
Beaverton, OR 97006
ph: (503) 690-5293
fax:(503) 690-5384




From daemon Tue May 23 00:29:34 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Mon, 22 May 2000 13:36:00 -0500
Subject: Re[2]:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Gary,

I have never used a sintillator type BSE detector, but the major differences
are
two fold.

Typically (though diodes are getting better all the time) the Robinson has a
better low energy BSE detection effiency. It follows that for the same
noise
level electronics, it would exhibit better sensitivity. The dynamic range
problem will still exist for very different Zs in the field of view. In the
middle and upper portions of the sensitivity range (low and lower), I would
not
expect much difference between them. ...Any Comments from users of both????

I like the 4 quad diodes since I can go differential mode for macro
topography
(like fracture surfaces) and show the gross features while suppressing the
fine
detail.

The Pt coating can have a profound negative effect on sensitivity if not
extremely thin. If given a choice, I would always use carbon for the best
BSE
sensitivity. ...If not hazardous, I would use Be, BUT!!!! For elements in
the
range of Al & Si, I prefer to use carbon and lower beam voltages to minimize
penetration, especially if they are films or very small features.

I once presented some data illustrating the BSE signal attenuation as a
function
of sputtered Au thickness. But that data would be hard to retrieve now.

Woody White
McDermott Technology





===================================================
I use a Robinson Model 6 which is specified at a Z contrast of 0.003
at } = 2KV. From your work with a 0.1Z detector, what would you say
is the qualitative effect that a higher Z contrast detector would offer?
I am especially interested in imaging Al/Si alloys and I typically
sputter coat them with Pt. I may try carbon one of these days to
see what the difference might be.

tnx,
gary g.



From daemon Tue May 23 00:29:37 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Mon, 22 May 2000 15:55:43 -0400
Subject: Re: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, heating in the sputter coater is likely the cause.

Confirm this by checking the coated samples under the optical
microscope before putting them in the SEM. This will eliminate from
consideration any artifacts caused by higher SEM vacuum and electron
beam damage.


At 2:56 AM -0400 5/22/00, lherault wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
425 E. University Ave.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Tue May 23 00:29:40 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 22 May 2000 16:30:51 -0500
Subject: Re: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are you using a magnetron sputter coater? If so, then you should not
be getting melting, etc. But ...

For how long did you coat your samples? You might try coating for
short periods, with some rest in between -- say 30 sec, turn off
(maybe add some argon to help carry away any heat), coat, rest, etc.
If your samples are very sensitive, use 10 sec. coat times. Rest for
30 sec more or less (empirically). I've done Teflon fabrics this way,
using 90 sec. coat periods without problems, but if your samples are
thick, this would increase the problems.

Phil

} I've had to coat some PLGA polymer samples and we think the coater may be
} melting them. The samples have a rolled or beaded edge and large cracks. I
} have jpeg images if anyone wants me to send them for a look. Are we
} correct in our assessment or should we look elsewhere for the source of
} cracks, shrinkage, beading around edges. These artifacts, BTW do not appear
} when the uncoated samples are checked under a light microscope.
}
} Thanks in advance.
}
} Ron L
} lherault-at-bu.edu

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue May 23 00:29:40 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 22 May 2000 14:35:24 -0700
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How long of running time, approximately?
Do you want dissolve from image to image or simple image swap?
Any audio?

gary g.


At 07:44 AM 5/22/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue May 23 00:29:40 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 22 May 2000 17:56:26 -0400
Subject: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ron:

If your samples are particularly heat sensitive, you should probably look
into an Ion Beam Sputtering System. The heating effects in such a system
are negligible. Of course, you have the added advantages of thinner, more
uniform coatings etc. also. We do make the IBS/e Ion Beam Sputter
Deposition and Etching System. If this is an infrequent application for
you, perhaps I can put you in touch with one of our local users who could
help you out.

Let me know if that would be helpful.

Best regards-

David
Writing at 2:45:39 PM on 05/22/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by "lherault"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I've had to coat some PLGA polymer samples and we think the coater may be
melting them. The samples have a rolled or beaded edge and large cracks.
I
have jpeg images if anyone wants me to send them for a look. Are we
correct in our assessment or should we look elsewhere for the source of
cracks, shrinkage, beading around edges. These artifacts, BTW do not
appear
when the uncoated samples are checked under a light microscope.

Thanks in advance.

Ron L
lherault-at-bu.edu

{



From daemon Tue May 23 00:29:41 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Mon, 22 May 2000 17:59:18 -0400 (EDT)
Subject: Used items for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




We are moving to a new building and need to get rid of some old equipment.
The following are available in New Jersey, USA. All items were fully
functional when last used, most have all accessories and owners manuals.
Any reasonable offer considered.

For sale:

-GW Electronics attachments to go on Hitachi HS-510: Dual magnification
unit, Graphics generator, and Homomorphic Processor

-LKB 8800 Ultrotome III (thermal advance, includes ALL accessories)

-Denton Critcal Point Dryer (CPD-1) With extra baskets, seals, and
coupling for CO2 tank

-Ames Lab-Tek cryostat (slow leak in refrigerant line)

-Miles Tissue Tek cryostat

-3 ea Sorval MT-1 Porter Bloom ultramicrotome
#15601 w/ pivoting telescopic mount, baseplate, B&L sterozoom
head, adjustable cold light source and light switch box

-Zeiss microspectrophotometer (MPM microscope photometer for
cytospectrophotometry) for Zeiss Photo-microscope, Ultraphot,
Standard Universal microscopes (includes light chopper, power
supply, monochromator, photometer head, photomultiplier housing,
indicator unit, and coupling for microscope head)

-Printz automatic print dryer model JET 260158 ferrotyper drum and canvas
belt in like-new condition)

-Kodak Ektamatic Model 214-K automatic print processor -Accessories for
AO/Reichert Microstar compound scope (available with or without
microscope): AO Expostar photomicrographic system (lens and
shutter, control unit model 1190, polaroid and 35 mm film backs),
AO verical illuminator for incident light fluorescence microscopy
(includes mercury lamp model 2054A, filter housing, AND
microscope), Camera lucida attanchment AO #1030

- 2 ea Omni-mixer homogenizer with stand model # 17105, 16,000 rpm, no
impellers -Lightnin Mixer Model F (no impellor)

-B&L Dynazoom microscope with integrated 4x5 (polaroid) and 35 mm camera
backs

**Items looking for a good home (shipping cost plus a little extra
for us to be able to say that we actually sold them):

-B&L # 42-63-89 projecting compound scope (used to do camera lucida)

-metal microslide mailers/holders by Thomas; holds 4 slides (approx 500
avaiable)

-stainless steel frames for holding individual sheets/plates of 3 1/4 x 4
EM film

--Arthur Thomas Co., paraffin for 50-52oc (33 lib pkg of 1/4 lb bars)


Dealers are welcomed to contact us. Perhaps we could give you some items
plus a cash allowance to purchase some used items that you may have that
we want.

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718






From daemon Tue May 23 00:29:41 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Mon, 22 May 2000 15:00:15 -0700 (PDT)
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can get NIH Image to do this and a great deal more. For PC's it's called
Scion Image, and it's available free from www.scioncorp.com. Hope this helps.

Lesley Weston.



On Mon, 22 May 2000, Kevin W. Eliceiri wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } I am also trying to figure out how to make a QuickTime video from a series
} } of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
} } in LSM format, which have been converted to Tiff. The number I am dealing
} } with is 64 images. Any help would be greatly appreciated
} } Linda Barthel
} } Research Associate II
} } Department of Cell and Developmental Biology
} } University of Michigan
} } barthel-at-umich.edu
}
} Our software, 4D turnaround will make quicktime movies out of image
} stacks that are in biorad format, pics, or tiff. Please see our
} website for more details and to download:
} http://www.loci.wisc.edu/4d/native/4d.html
}
} Currently we have only a mac version available, however we will be
} releasing a java version next month that works cross platform.
}
} Best regards,
} kevin
}
} Kevin W. Eliceiri
} Project Director
} Laboratory for Optical and Computational Instrumentation
} http://www.loci.wisc.edu
} 159 Animal Sciences
} 1675 Observatory Dr.
} Madison, WI 53706
} 608-263-6288 voice
} 608-265-4076 fax
}
}
}



From daemon Tue May 23 00:29:50 2000



From: frieda lim :      barbaricyawp-at-worldnet.att.net
Date: Mon, 22 May 2000 18:40:26 -0400
Subject: Re: Query for microscopic organism video/filmfootage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


: Frieda Lim
} Barbaric Yawp
} 224 west 13th Street #5r
} NYC, NY 10011-7775
} 212.206.0683
} barbaricyawp-at-att.net
}
} Dear MSA Members
}
} It was suggested to me that this may be the perfect spot to list my query.
} I would appreciate your perusal of the below queries and am anxious to
hear from you.
}
} Sincerely,
} Frieda Lim
}
}
}
} 1st query:
}
} Dear MSA Members :
}
} I invite you to take a close look at our query. We are a film production
} company seeking existing moving microscopic organism film or video
footage.
} We will utilize this footage for our single purpose of making a feature
} film. The film we are creating is a microscopic fantasia. Zooming in on
} your microscopic world, we will spin a tale through imagery and music.
}
} We would appreciate your help with obtaining some footage directly or any
} referring leads. Please contact us at your convenience to discuss further
} details.
}
} Sincerely,
} Frieda Lim
}
}
}
}
} Elaborating 2nd Query:
}
} Dear MSA Members:
}
} I thank you for your prompt response to my query for microscopic
} microorganism footage. Our intended use of your existing footage is to
edit
} & generate a full feature film comprised solely of microscopic imagery and
} music with which we hope, eventually, will be distributed to the general
} public at large--large in fact by mesmerizing all ages, the young & the
} young of heart. Our microscopic fantasia will bring to life a single
} narrative, the classic myth of Cupid & Psyche.
}
} In our original query, we had cast a wide net being intentionally
} non-specific with regard to the kind of microlife we are seeking. At this
} stage, we have no restrictions as to what microworld would be best suited
} for our story. However, our intent is to portray our story within a
} scientific veritable reality. We don't want micro species colliding that
} would never interact in truth. Right now we are in our initial phase of
} investigative hunting & gathering-a casting call for microscopic actors.
We
} actually want to cast organisms as actual characters and to use others as
} metaphorical imagery. My suspicion is that you have already created
footage
} for your scientific research and discovered many a talented organism
} awaiting their big break. We further suspect that we will need to pool
} microscopy resources and see what footage offered is most varied in look &
} movements that will best fill the wide assortment of roles required.
Since
} we are approaching your world as layman, we would appreciate your
expertise
} in advising what microorganism microcosm might be most readily available.
}
} To note, this is a low budget project with big hopes and dreams. We
} believe our film can achieve success similar to that of "Microcosmos"-the
'
} 96 Cannes Film Festival jury prize winner. That movie was testament that
} science has mass marketable enthusiasm in the entertainment world.
} Comparatively, we hope to explode your frontier world onto the big screen
} and excite a wide audience.
}
} So, if you know of some microorganisms wanting to make it big, we are
} anxious to hear from you. Thank you.
}
} Sincerely,
} Frieda Lim





From daemon Tue May 23 00:30:05 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 22 May 2000 17:43:59 -0700
Subject: quicktime

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Tue May 23 00:30:05 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Mon, 22 May 2000 18:55:44 -0600 (Hora est‡ndar de MŽxico)
Subject: starch granule success

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

Firstly a confession, that my taxonomy is badly in need of repairing - the
alga we have been working with is not a blue-green algae at all - it's
actually Polytomella, a eukaryote, and I think is a relative of
Chlamydomonas, but doesn't have a cell wall or photosynthetic apparatus.

Anyway, embedding in Spurr's seems to have done the trick as far as
stopping the starch granules dropping out. I've just been looking at
grid-fulls of cells full of starch granules, and they're all there! Many
thanks for the suggestions. I'll be back with more problems soon!

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Tue May 23 01:07:35 2000



From: Orion Analytical :      orionanalytical-at-worldnet.att.net
Date: Mon, 22 May 2000 21:42:45 -0400
Subject: microscopy and authentication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret -

We've applied microscopic techniques (e.g., polarized light microscopy,
fluorescence microscopy, FTIR microspectroscopy, and SEM-EDS) and
non-microscopic techniques to more than 700 projects involving historic and
artistic works, from Egyptian antiquity to contemporary. Material evidence
of authenticity -- or more likely, inauthenticity -- has been or become an
objective of many of these studies.

Scientific investigation plays an important role in the authentication
process, often providing indirect or direct evidence of date, and an
objective basis by which to compare materials and techniques to works of
unquestioned authenticity. Given sampling limitations and the layered
structure of many art objects and decorative finishes, microscopy is ideally
and elegantly suited to their study of the former - that is, structure and
composition. We use a new infinity-corrected optical/FTIR microscopy
system, that we had custom-fitted for polarized light microscopy,
fluorescence microscopy, and FTIR microspectroscopy (narrow-band MCTA and
wide-band MCTB detectors).

Your article sounds very interesting -- please feel free to call.

Jamie

James Martin
Orion Analytical, LLC
Post Office Box 550
Williamstown, MA 01267
phone: 413-458-0233
e-mail: martin-at-orionanalytical.com
website: www.orionanalytical.com



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am new to this list. I would like to know if anyone has information I
could use to write an article on the analytical chemistry techniques used to
authenticate art objects using light microscopes or any other type of
microscope. Also, if anyone has information on any of the other techniques
used to authenticate art objects such as x-ray diffraction, x-ray
fluorescence, accelerator mass spectrometry, that would be helpful too.

Sincerely,

Margaret M. Mitchell
Assistant Editor
AOAC INTERNATIONAL
301-924-7077 (tel)
301-924-7089 (fax)
mmitchell-at-aoac.org




From daemon Tue May 23 01:07:35 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 22 May 2000 22:15:15 -0500
Subject: Sectioning Ni-Zn-P coatings

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jean Dille wrote:
=====================================================
We have a sample of 6 micrometers Ni-Zn-P electroless plated onto 1 mm
thick Al and we would like to prepare cross sections for TEM examination.
It looks very difficult to use the tripod polishing technique for metals.So,
we
are thinking at an electropolishing procedure with our double-jet Tenupol
apparatus.

This would be done on slices cutted from a sample initially thicked up to
3mm
by electroplating.But how to avoid differential material removal problems?
Which material for plating?Which electrolyte for electropolishing?Other
method?

Does anyone have suggestions for us?

Thanks in advance for your help.
====================================================
If the aluminum substrate can be thinned down a bit more, then a coating of
this thickness should be able to be thin sectioned using diamond knife
ultramicrotomy. It is hard to predict whether better results will be
obtained embedded or unembedded (there is a tendency for the coating to
separate from the substrate). We usually find that such separation is less
likely to occur if the sample is embedded. However, structure within the
coating is less well preserved when embedded. Naturally our experience has
been with our own SPI-Pon™ 812 epoxy embedding resin and SPI diamond knives,
but I would expect that at least most of the other "Epon substitutes" (and
knives) would work just as well. With regard to the substrate separation,
the tendency for this to happen can be reduced by using a knife included
angle that is smaller rather than larger (we were successful with 45 deg.).
The use of larger included angles, at least in our experience, seemed to
produce a level of compression artifacts in the sections that we found
unacceptable.

In any case, we have found the diamond knife thin sectioning approach to
often times offer certain advantages over the alternatives for sample
preparation.


Disclaimer: SPI Supplies offers materials science diamond knives and also
our preferred embedding resin, SPI-Pon 812 Embedding System. Our Structure
Probe™ laboratory services division performs this kind of diamond knife thin
sectioning as a service for commercial clients.

Chuck

PS: Remember that we are trying to become 100% paperless and the only way
that we can manage this kind of correspondence, in a paperless environment,
is for our correspondents to always reply by way of "reply" on their
software so that the entire string of correspondence on that topic gets
returned to us, and the entire correspondence history can be kept in one
place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Tue May 23 01:07:35 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 22 May 2000 22:44:44 -0500
Subject: PLGA coating

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ronald J. L'Herault wrote:
====================================================================
I've had to coat some PLGA polymer samples and we think the coater may be
melting them. The samples have a rolled or beaded edge and large cracks. I
have jpeg images if anyone wants me to send them for a look. Are we
correct in our assessment or should we look elsewhere for the source of
cracks, shrinkage, beading around edges. These artifacts, BTW do not appear
when the uncoated samples are checked under a light microscope.
====================================================================
If by PLGA you mean polylactic glycolic acid, which in certain forms could
be a (dissolvable) surgical suture material, then the polymer could be quite
hygroscopic and if it has had time to absorb enough moisture, it could be
evolving moisture, and that could be the reason for your problems.

Some sputter coaters have a "test mode" which enables the user to
momentarily expose in a gentle way the polymer surface to the glow of the
plasma. If moisture is evolving, then there will be an immediate diminution
of the quality of the vacuum. When confronted with that kind of situation,
we go through the cycle of "test mode", then pump down, test mode, etc,
until hitting the test mode button results in no more deflection of the
vacuum. Ten or more cycles might be needed but in the end, the surface
moisture is removed.

Then you are ready to coat.

If you are not talking about polylactic glycolic acid, I apologize for
taking up the extra bandwidth.

I realize that not all sputter coaters have such a test mode, but that is
the perfect kind of example where such a "test mode" has its greatest value.

Disclaimer: SPI Supplies manufactures sputter coaters with "test modes".

Chuck

PS: Remember that we are trying to become 100% paperless and the only way
that we can manage this kind of correspondence, in a paperless environment,
is for our correspondents to always reply by way of "reply" on their
software so that the entire string of correspondence on that topic gets
returned to us, and the entire correspondence history can be kept in one
place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================










From daemon Tue May 23 01:44:47 2000



From: Orion Analytical :      orionanalytical-at-worldnet.att.net
Date: Tue, 23 May 2000 00:54:27 -0500
Subject: microscopy and authentication of art objects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret -

I've applied microscopic techniques (e.g., polarized light microscopy,
fluorescence microscopy, FTIR microspectroscopy, and SEM-EDS) and
non-microscopic techniques to more than 700 projects involving historic and
artistic works, from Egyptian antiquity to contemporary. Material evidence
of authenticity -- or more likely, inauthenticity -- has been or become an
objective of many of these studies.

Scientific investigation plays an important role in the authentication
process, often providing indirect or direct evidence of date, and an
objective basis by which to compare materials and techniques to works of
unquestioned authenticity. Given sampling limitations and the layered
structure of many art objects and decorative finishes, microscopy is ideally
and elegantly suited to their study of the former - that is, structure and
composition. We use a new infinity-corrected optical/FTIR microscopy
system, that we had custom-fitted for polarized light microscopy,
fluorescence microscopy, and FTIR microspectroscopy (narrow-band MCTA and
wide-band MCTB detectors).

Your article sounds very interesting -- please feel free to call.

Jamie

James Martin
Orion Analytical, LLC
Post Office Box 550
Williamstown, MA 01267
phone: 413-458-0233
e-mail: martin-at-orionanalytical.com
website: www.orionanalytical.com



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am new to this list. I would like to know if anyone has information I
could use to write an article on the analytical chemistry techniques used to
authenticate art objects using light microscopes or any other type of
microscope. Also, if anyone has information on any of the other techniques
used to authenticate art objects such as x-ray diffraction, x-ray
fluorescence, accelerator mass spectrometry, that would be helpful too.

Sincerely,

Margaret M. Mitchell
Assistant Editor
AOAC INTERNATIONAL
301-924-7077 (tel)
301-924-7089 (fax)
mmitchell-at-aoac.org




From daemon Tue May 23 01:44:48 2000



From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Tue, 23 May 2000 01:08:52 -0500
Subject: QuickTime Videos from digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Edgar,

When I purchased a copy of Adobe Photoshop 5.5, it came bundled with their
"ImageReady 2.0" This package can be used to create animated GIF images
from layered images, or the animation exported in QuickTime format.

Regards,

Neal




From daemon Tue May 23 01:44:47 2000



From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Tue, 23 May 2000 01:09:20 -0500
Subject: QuickTime Videos from digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Edgar,

When I purchased a copy of Adobe Photoshop 5.5, it came bundled with their
"ImageReady 2.0" This package can be used to create animated GIF images
from layered images, or the animation exported in QuickTime format.

Regards,

Neal




From daemon Tue May 23 01:44:48 2000



From: Angela Klaus :      avklaus-at-amnh.org
Date: Tue, 23 May 2000 01:09:38 -0500
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Linda,

What I ended up doing in your situation was downloading Confocal Assistant,
and reconstructing the TIFF series using that. You first need to add a
BioRad header... the software and tricks can be found at:

http://www.cs.ubc.ca/spider/ladic/source.html

You can export the animation from CA as an .avi file. Then if you have
Image Ready (Photoshop 5.5), you can convert the .avi to .mov. (I think
Graphics Converter will also work) A roundabout way to get the job done,
but it works.

All the best,

Angela

} I am also trying to figure out how to make a QuickTime video from a series
} of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
} in LSM format, which have been converted to Tiff. The number I am dealing
} with is 64 images. Any help would be greatly appreciated
} Linda Barthel
} Research Associate II
} Department of Cell and Developmental Biology
} University of Michigan
} barthel-at-umich.edu
}
}
}
}
}
}
---------------------------------------------
Angela V. Klaus

Laboratory Manager, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977, 5469
Fax: 212-496-3480
---------------------------------------------




From daemon Tue May 23 05:15:12 2000



From: HARRISm-at-esm-semi.co.uk
Date: Tue, 23 May 2000 10:02 +0000 (GMT)
Subject: Equipment reliability

Contents Retrieved from Microscopy Listserver Archives
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A how long is a piece of string ? type question


I would be pleased if anyone could broaden my views on equipment
reliability .
Basically we have a 3yr old FESEM which I consider to be fairly
reliable in that it is on call 24hrs/day , has numerous ( non
dedicated users ) and apart from downtime for filament change and the
odd wear and tear type problems answers our needs .
As we do not have a back up instrument and when we do experience
problems it's always at the worse time certain personnel have the
impression that it is unreliable .

What do other sem users expect in terms of reliability , apart from my
' subjective ' comments is it quantifiable , would approx 2wks /year
downtime including planned maintenance be considered excessive ?

Regards
Martyn Harris
harrism-at-esm-semi.co.uk



From daemon Tue May 23 08:08:13 2000



From: Geoff Williams :      Geoffrey.Lloyd.Williams-at-cmich.edu
Date: Tue, 23 May 2000 08:12:56 -0400
Subject: Re: video from single images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have done this procedure many times using many different programs.
Most often I was using TIFF files in sequence. I can't remember all the
names
of the programs that I tried, most were freeware or shareware including
NIH
image and the results were never to my satisfaction, I wanted control
over
Frames per second, compression, output format, light levels, you know
complete
control.
The program that I wound up using was Adobe After Effects. An
incredible
program, capabilities way beyond what was needed for creating simple
sequence
movies from 3D volumes captured in a LSCM and rendered on a SGI. The
benefit is
unlimited file type importation (well nearly unlimited - hey it supports
SGI
.rgb files!) and complete control over the creation process. I could
make an
animated gif with any frame rate I wanted or a full compression-less
Quicktime
(.mov) or Microsoft Video for Windows format (.avi) with sound and
special
effects.

Most of you have used Adobe photoshop at one time or another, Adobe
After
Effects has the same intuitive interface and many of the same filters
and image
adjusting features. The educational discount for the lab brought the
price down
to less the $300! An incredible value for anyone doing lots of routine
slice
parade movies or animations for presentations.

If you have the ability to record sound on your computer you can even
record a
whole seminar presentation. Imagine showing up at a meeting plugging in
your
laptop computer (with sound) to the projection system and clicking
play. You
can sit down and listen to your own presentation, and then answer
questions at
the end. No more forgetting to mention a point, you can make sure that
you are
making sense. Of course this all takes away from the art of the
presentation.
I have never done this mind you all but the idea is intriguing isn't it?

Adobe also has a software program called Premier that is used in the
film
industry to do even more digital effects and post production (I think
the last
guess I heard was that about 75% of all rolling credits and film intros
are done
with this program and many of the same features are in After Effects).

-Geoff

--
Geoff Williams, M.S.

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Tue May 23 08:08:12 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 23 May 2000 07:18:40 -0700
Subject: Re: TEM: Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon Couger wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } From: "Tina Carvalho" {tina-at-pbrc.hawaii.edu}
} } On Thu, 18 May 2000 ComCryLab1-at-aol.com-at-sparc5.microscopy.com wrote:
} }
} } } Dear Friends,
} } } Can anyone give me advice regarding the dismantling and
} } } the packing for shipment of a Philips 201 TEM? I need to
} } } pay particular attention to not disturbing the alignment.
} } } I plan to move the TEM from Univ. of Delaware to Naples, Florida
} } } in an enclosed U-Haul Trailer. Any help or suggestions
} } } will be most welcome.
} }
} } I will make sure Ron Veil, a very experienced independent EM service tech,
} } sees this and has a chance to reply. It was with his advice that we (my
} } hubby and I and its new owner) packed up and shipped a Philips 201. Like
} } you, we wanted to ship it intact, column on and all. We built a heavy
} } duty skid and added a strong base to which we bolted the instrument. Then
} } we wrapped it with whatever that plastic packing tape that is like Saran
} } Wrap is called, making sure to secure any part of the column we didn't
} } want to move, but avoiding putting any pressure on things, such as the
} } aperture drives. Wrap the bottom, wrap the column, wrap the column to the
} } bottom and back again, etc. Build a crate up around the instrument.
} } I think I remember putting a wood insert with a crescent cut out near the
} } top of, but not touching the column, but hubby thinks not. The idea is
} } NOT to let any shock to the crate get transferred to the column, so
} } perhaps we didn't. Add some packing material, such as old egg crate foam
} } from your bed (it needed replacing anyway) and whatever. Then, and this
} } was the fun part, buy that stuff that when you mix it with a catalyst,
} } produces huge volumes of foam that hardens in a few minutes. I think you
} } can also buy spray cans of similar stuff, but since hubby had this on hand
} } for other things, we had the bulk stuff. Fill the voids in the crate with
} } it. It will easily chip off later. Add a top, and away you go. The
} } scope made it in great shape.
} }
} } I think we packed the rotary pump and a couple of other things
} } separately. Get it into the truck and TIE IT DOWN. I say this because an
} } SEM we once packed for shipping with the base and saran, but no closed
} } crate, made it from Hawaii to Ohio, then slid in the shippers truck in the
} } last mile and slammed into the driver's seat. The driver was OK, the ion
} } getter pump was bent, and the column needed a bit of work, but it could
} } have been worse. Duh.
} }
} } I've also packed up a couple of Denton vcuum evaporators and a couple of
} } ultramicrotomes. The saran stuff and blow foam are a good way to
} } stabilize the instruments.
} }
} Since you are using a U haul you don't have to worry about sides
} and a top on the crate. Make sure it is tied down real well. Dry
} wall screws through the crate and into the floor work well for
} locking it down but make sure you have it braced with timbers
} to the front and sides of the trailer in case you make a panic
} stop.
}
} Also make sure that the weight is centers in front of the trailer
} axle if you use a trailer. Negitive weight on the trailer tounge
} at the very least makes driving very interesting. You have no
} Idea how fast a trailer can pass you while it is still tied on to the
} truck. I have had the privilege of experiencing this and I can promise
} you that you won't enjoy it:).
}
} The foam in place stuff is great. Just cover the instrument in plastic
} and foam away. If you can get foam between the instrument and the
} Support the foam will dissipate most of those little shocks that get
} things out of line.
}
} Last of all make sure you understand what you insurance covers and
} what it doesn't on moving equipment. You should be fine on liability but
} the value of the contents is probably not covered. Rental truck companies
} have contents insurance as an extra.
}
} Good luck
} Gordon
}
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00


Gordon,
Now I guess I know who to blame when I get U-Haul trailers that are in
p----poor shape to move SEMs. Where do you get off running screws
through the floor of a trailer you don't own?

Unbelievable!

Ken Converse
owner
Quality Images
Delta, PA



From daemon Fri May 26 11:55:53 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 26 May 2000 08:13:37 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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You could probably expose your CCD chip directly to the electron bean --
and buy a new chip every few hundred exposures or so! Electrons have
mass and carry momentum, which gets transformed into force as they are
stopped in the crystal, especially at higher keV. This can lead to
damage. Photons only heat up the target.

As far as I know ALL TEM cameras use a medium to convert electrons into
light, and this light is then detected. The medium is either a phosphor
screen or a very thin YAG crystal. From there on it is different. Some
cameras use a fiber-optic to guide the photons to the chip, others use
mirrors and lenses. So, it is not simply a matter of comparing the QE of
the chip, the entire system has to taken into account. Also, each
electron creates many photons in the phosphor or YAG, and in principle
only one photon is enough to create a signal in the CCD. In other words,
a CCD could theoretically detect "fractions" of an electron, whereas the
film is directly exposed to the electron. Of course the electron can
"rattle" around in the film and activate several grains, but as you can
see, the question of comparing sensitivities now becomes one of
comparing two different physical processes. It can definitely be done,
but it's not easy.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: hpadams [mailto:hpadams-at-bcm.tmc.edu]
Sent: Thursday, May 25, 2000 12:30 PM
To: microscopy-at-sparc5.microscopy.com


I have been following off and on this discussion on CCD cameras for EM.
Much of this discussion has been concerned with "sensitivity". I am
being
naive here, but when we talk about "sensitivity" of CCDs, isn't this the
same
thing as the QE of camera/chip? I don't think I have ever seen a QE for
em
CCD's for various accelerating voltages/wavelenghts. Does the electron
beam
directly hit the silicon photodyodes or it there an interface that
converts
the incoming electrons to different (longer?) wavelengts? I know em
films are
sensitive to specific acc voltages. Are CCD cameras for em the same. For
long
exposures it may not matter, but for short exposures or low level
intensity
does the QE of the camera come into play?

} ===== Original Message From Jenichen {Jenichen-at-proscan.de} =====
} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri May 26 11:55:53 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Fri, 26 May 2000 09:40:16 -0500
Subject: cathodoluminescence?

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Hello all,
I have a researcher that is interested in utilizing florescent probes to
label material with the desire to visualize with a 50 angstrom
resolution. Are there any service facilities out there that have a
cathodoluminescence detector on their SEM or STEM that would be able to
assist him (via contract)?
Thank you!


Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Fri May 26 11:55:54 2000



From: Paul Voyles :      voyles-at-research.nj.nec.com
Date: Fri, 26 May 2000 11:11:23 -0500
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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} You could probably expose your CCD chip directly to the electron bean --
} and buy a new chip every few hundred exposures or so! Electrons have

There is an even worse problem with exposing the CCD directly the
electron beam. The p-n junctions in the CCD chip have a certain
"well capacity" - a number of electron/hole pairs they can hold
before they saturate. Fast (keV) electrons are much more efficient
at producing electron/hole pairs than photons - so much so that a
pixel on the CCD would saturate after about 15 fast electrons hit it.
Just the square root N shot noise at that level is about 25% - larger
than typical TEM micrograph contrast of 10-20%. That's why a
phosphor or scintillator is necessary to transform the fast electrons
into photons.

In case anyone is interested, you can learn more than you ever
possibly want to know about CCD cameras in:

"Applications of slow-scan CCD cameras in transmission electron
microscopy" O. L. Krivanek and P. E. Mooney, Ultramicroscopy V. 49 p.
95-108 (1993). First description of Gatan CCD cameras, including
some design issues, and measurements of the linearity, modulation
transfer function (MTF), and detector quantum efficiency (DQE).

"Methods to measure the properties of slow-scan CCD cameras for
electron detection" W. J. de Ruijter and J. K. Weiss, Rev. Sci.
Instrum. V. 63, p. 4314 - 4321 (1992). Another early paper, includes
comparisons to photographic plates. Uses a slightly different
definition of the MTF from everyone else.

J. M. Zuo "Electron detection characteristics of slow-scan CCD
camera", Ultramicroscopy V. 66 p. 21-33, (1996). Describes gain,
MTF, and DQE measurements, measurement techniques based on stochastic
noise (blank beam) images, and theory. This is a good place to start.

"Quantitative characterization of point spread function and
detection quantum efficiency for a YAG scintillator slow scan CCD
camera" A. L. Weickenmeier, W. Nuchter, and J. Mayer, Optik, V. 99,
p. 147-154, (1995). Line-scan method of measuring the MTF.


Happy reading,
Paul




Paul Voyles, voyles-at-research.nj.nec.com
voice: (609) 951-2627, fax: (609) 951-2496
NEC Research Institute
4 Independence Way
Princeton, NJ 08540 USA
{http://www.neci.nj.nec.com/homepages/voyles/fluct.html}



From daemon Fri May 26 11:55:56 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 26 May 2000 07:49:00 -0500
Subject: RE: BSE Information

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Victor,

On which systems have you observed higher focused beam current from the
false
(first) saturation peak rather than "true" saturation? I have not observed
this
in 18 years with an Etec nor on the new Hitachi.

Woody White

-----------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi friends
I agree Steve, sometimes (I seem it depends on electron gun design and
distance between wenelt and anode, wenelt and filament) the first peak
on saturation curve is more than saturation level even in secondary
emission signal.
Victor Sidorenko, ANTRON Co.Ltd., Moscow, Russia


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---
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America



From daemon Fri May 26 11:55:56 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 26 May 2000 07:42:00 -0500
Subject: RE: BSE first sat peak

Contents Retrieved from Microscopy Listserver Archives
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I would also be interested in learning how/why. I understand that if the
larger
spot size delivers more incident beam current, signal to noise is improved,
but
this does not seem to be the parameter to which Steve is referring.

Woody White
McDermott Technology

-----------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


At 05:07 PM 25-05-2000 +0100, you wrote:
Steve Chapman wrote,
SNIP
, then by de-saturating the larger source
} will result in a larger probe dimension on the specimen; increasing the
} probe diameter increases the volume of material involved in the production
} of BSE.
}
Ken Wrote:
But how does this lead to an increase in the BSE differentiation (implying
more signal and hence less noise). Surely just defocussing would do the same
thing. Defocussing as such should not effect the BSE coeffecient, unless you
had sub surface charging or some other artefact.

Very interesting!
Ken.



From daemon Fri May 26 11:55:57 2000



From: COURYHOUSE-at-aol.com
Date: Fri, 26 May 2000 12:10:30 EDT
Subject: Re: Service Manuals

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Dear Walter, yes we have started one. We continue to gather as time goes on
many of them are stored at the moment as the library section that will house
them will not be finished until November. we are planning to offer some of
them online in pdf format when we have permission from the various
companies on the really old ones that they seem not to care about.

We need anything we can get our hands on and also include in this pursuit
their older equipment brochures and catalogs. Any assistance of originals or
good Xerox copies is great!

thanks Ed Sharpe archivist for SMECC

{ { Subj: Service Manuals
Date: 5/25/00 10:54:00 PM US Mountain Standard Time
From: Corvos-at-aol.com-at-sparc5.microscopy.com
To: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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All,

I would like to know if any has started an extensive collection of
Microscopy
Service Manuals?

Regards,

Walter Protheroe
E-MAC, Inc.
} }



From daemon Fri May 26 11:55:57 2000



From: rnatarajan :      rnataraj-at-hd2.dot.net.in
Date: Fri, 26 May 2000 22:20:17 +0530 (IST)
Subject: Accomodation at M&M 2000

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Dear fellow researcher,

I am an author and platform presenter at the M&M 2000 conference at Philadelphia in August,2000. I am a scientist at the National Geophysical Research Insitute , India. I am looking for living accomodation during my 5 day stay at Philadelphia. I was wondering if any of you will be able to provide me with free accomodation or would be aware of any other resources who may be able to help me in this regard. Due to the limited fiancial resources avaliable to me from my lab, I am looking forward to help from my fellow researchers. Hope you can help me in this regard. I am eagerly looking forward to contributing to the M&M 2000 conference.

With kind regards
R. Natarajan
Scientist, National Geophysical Research Institute, Hyderabad,
India
e-mail: rnataraj-at-hd2.dot.net.in
Phone: 91-40-7170141 X 2430 (W)
Fax: 91-40-7170564



From daemon Fri May 26 17:02:34 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Fri, 26 May 2000 13:25:37 -0400
Subject: Light Microscope: Looking For a Leitz....

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I am looking for a 170mm Leitz body tube lens (a field lens as Leitz called it) for a Leitz
Ortholux.

This is the lens that is inside the nose turret.

The engraving on the lens is 170/223, 1.25 W

If you have any older Leitz items to sell please let me know.

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net



________________________________________________________
1stUp.com - Free the Web
Get your free Internet access at http://www.1stUp.com



From daemon Fri May 26 17:02:34 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Fri, 26 May 2000 10:02:27 -0800
Subject: Thanks for Varian and ISI help

Contents Retrieved from Microscopy Listserver Archives
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A hearty thank you to everyone who replied to my request for help. I had
sources for everything I needed before the evening was out. It is the
generosity of the subscribers that makes this list-serve a great
resource. Thanks again.

Kim DeRuyter
Electron Microscopy Technician
308 Natural Science Facility
P.O. Box 755780
University of Alaska
Fairbanks, AK 99775-5780
907-474-5452
907-474-5163 fax



From daemon Sat May 27 12:37:36 2000



From: rnatarajan :      rnataraj-at-hd2.dot.net.in
Date: Sat, 27 May 2000 07:54:59 +0530 (IST)
Subject: Accomodation at M&M 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear fellow researcher,

I am an author and platform presenter at the M&M 2000 conference at Philadelphia in August,2000. I am a male scientist at the National Geophysical Research Insitute , India. I am looking for living accomodation during my 5 day stay at Philadelphia. I was wondering if any of you will be able to provide me with free accomodation or would be aware of any other resources who may be able to help me in this regard. Due to the limited fiancial resources avaliable to me from my lab, I am looking forward to help from my fellow researchers. Hope you can help me in this regard. I am eagerly looking forward to contributing to the M&M 2000 conference.

With kind regards
Mr. R. Natarajan
Scientist, National Geophysical Research Institute, Hyderabad,
India
e-mail: rnataraj-at-hd2.dot.net.in
Phone: 91-40-7170141 X 2430 (W)
Fax: 91-40-7170564



From daemon Sat May 27 12:57:55 2000



From: Tony Kowal :      askowal-at-midway.uchicago.edu
Date: Sat, 27 May 2000 12:38:36 -0500
Subject: Tissue Drier

Contents Retrieved from Microscopy Listserver Archives
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Dear Fellow Microscopists -
Someone in the lab in which I work wants to do SEM of yeast colonies. I
was able to locate some references and a general protocol, which seems
rather straight forward. However, the authors two of the papers refer to
an "Edwards Pearce Tissue Drier, EPD3". I contacted Edwards to find out
what this tissue drier is, however they said they could not give me any
info because it is no longer manufactured.
I am thinking that this tissue drier is simply a freeze drier, but I am not
sure. Does anyone out there have any insight into what this thing is or
what it does? Thanks for your help!!

Tony


Tony Kowal
Research Assistant
for
Dr. Susan Lindquist

Howard Hughes Medical Institute
The University of Chicago
5841 S. Maryland
MC 1028, Room N339
Chicago, IL 60637

Phone: (773) 702-8795
Fax: (773)702-7254
e-mail: askowal-at-midway.uchicago.edu
Pager: on campus - 188 - 9668 (YNOT)
off campus - (773)753-1880 - 9668




From daemon Sun May 28 08:28:16 2000



From: Pbgrover-at-aol.com
Date: Sat, 27 May 2000 16:19:18 EDT
Subject: charred plant seeds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Esteemed colleagues,

A student has asked me if I can find a way to tell whether small plant seeds
have been charred or not. She is examining seeds from an anthropological dig
on a site probably inhabited both in historic times (170 years ago) and
prehistoric (1000+ years ago). It seems that for seeds to date from the
earlier inhabitation, they would need to be charred, which apparently
preserves them.

I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of same
species as controls. The seeds of unknown age showed a lot more charging
than the fresh ones. Could this be evidence that they are uncharred (i.e.,
would charring make them more conductive/better secondary electron
producers?) Does anyone know of a method of differentiating between charred
& uncharred seeds?

Thank you thank you thank you :0)

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN



From daemon Sun May 28 08:38:23 2000



From: BENJAMIN HIDALGO :      bhidalgo-at-ci.udo.edu.ve
Date: Sun, 28 May 2000 08:29:20 -0500
Subject: I NEED SOME INFO

Contents Retrieved from Microscopy Listserver Archives
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Hello anybody from the ListServer: I need to have information on the
approximate market price of a HITACHI S-800 FE SEM. Thank you.
Ben Hidalgo-Prada




From daemon Sun May 28 15:07:28 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Mon, 29 May 2000 08:44:21 +0200
Subject: Sample prep: ceramics

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The Edwards Pearce Tissue drier was a freeze drier with a small
peltier cooled specimen stage. The peltier device was a water-
cooled 3-stage stack, achieving about -60oC on a stage about the
size of a 35mm negative. There was provision for a small tray of
phosphorus pentoxide, and the specimen chamber was a simple
Pyrex glass bell, pumped by a rotary pump.

Date sent: Sat, 27 May 2000 12:38:36 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: Tony Kowal {askowal-at-midway.uchicago.edu}


How about using the low angle cleaving method of Rafferty? Polish with the
SiC close to say the {110} planes (e.g. ten degrees) and then cleave along
both the scratches and the {110} plane to get a thin acute wedge. It will
take some practice, but should work generally with most Perovskites.

references:
1/Thin Solid Films Vol308-309 (1997) pp399-405
S.D.Walck & J.P.McCaffrey: The small angle cleavage technique applied to
coatings and thin films

2/Thin Solid Films Vol304 (1997) pp157-159
Suli Suder, C.A.Faunce & S.E.Donelly: Thin solid film preparation by a
small-angle cleavage for transmission electron microscopy

-I hope thses can provide some help. By the way this is not a definitive
list, but I am sure Scott Walck can give you a far greater insight to this
method.

Regards, Jon

********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Mon May 29 05:50:02 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 29 May 2000 11:33:16 +0000
Subject: Re: Tissue Drier

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Chuck
CPD certainly did become the most popular method by a country
mile for SEM specimen drying, but freeze-drying still has
advantages over it in some situations. These include, for example,
specimens where lipid content or lipid structures must be retained
(e.g. plant and insect epicuticles), where the specimen is an
aggregate of objects loosely bound by a fluid or a mucilaginous
matrix (e.g. it could be an advantage in Tony Kowal's yeast and
bacterial colonies, soils and clays), or where the specimen is
mechanically fragile and the components would be dispersed on
submersion in baths of liquid during fixation and solvent drying and
CPD (soils & clays, fungal sporangia, yeast and bacterial colonies).

The down side of freeze-drying in most of these contexts is that
some shrinkage and distortion almost always results.
Consequently, for almost all the situations listed above, and a host
of others as well, Low-temperature SEM became the method of
choice. In LTSEM the specimen can be viewed fully frozen-
hydrated, but most commercially-available LTSEM systems have
specimen temperature control, and full or partial freeze-drying can
be undertaken either on the SEM specimen stage or in the cryo-
preparation unit if required.

Anyone seeking a freeze-drier unit for EM specimens should
contact Emitech who make a peltier-cooled unit (K750) which
operates around -60oC (and is not unlike the Edwards-Pearce
tissue drier) and a turbo molecular pumped Liquid nitrogen cooled
low-temperature freeze drier (K775) which operates {-80oC.

http://www.Emitech.co.uk/

I have no financial interest in this company

Chris


} Hi Chris,
}
} Am I not remembering correctly, or was this earlier approach sort of a
} precursor to the critical point drying technique? I mean, did not people use
} this earlier technique because that was all they knew and then when the
} option of CPD came along, everyone switched over to it?
}
} I myself was reluctant to say that because that was a very long time ago and
} the memory does dull at the edges.
}
} Chuck
} -------- REPLY, Original message follows --------
}
} } Date: Sunday, 28-May-00 08:57 PM
} }
} } From: Chris Jeffree \ Internet: (cjeffree-at-srv0.bio.ed.ac.uk)
} } To: Tony Kowal \ Internet: (askowal-at-midway.uchicago.edu
} )
} } cc: MICROSCOPY BB \ Internet:
} } (microscopy-at-sparc5.microscopy.com)
} }
} } Subject: Re: Tissue Drier
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To
} } Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-
} Line
} } Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } The Edwards Pearce Tissue drier was a freeze drier with a small peltier
} cooled
} } specimen stage. The peltier device was a water- cooled 3-stage stack,
} achieving
} } about -60oC on a stage about the size of a 35mm negative. There was
} provision
} } for a small tray of phosphorus pentoxide, and the specimen chamber was a
} } simple Pyrex glass bell, pumped by a rotary pump.
} }
} } Date sent: Sat, 27 May 2000 12:38:36 -0500
} } To: Microscopy-at-sparc5.microscopy.com
} } } From: Tony Kowal {askowal-at-midway.uchicago.edu}
} } Subject: Tissue Drier
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Fellow Microscopists -
} } } Someone in the lab in which I work wants to do SEM of yeast colonies. I
} } } was able to locate some references and a general protocol, which seems
} } } rather straight forward. However, the authors two of the papers refer
} to
} } } an "Edwards Pearce Tissue Drier, EPD3". I contacted Edwards to find out
} } } what this tissue drier is, however they said they could not give me any
} } } info because it is no longer manufactured.
} } } I am thinking that this tissue drier is simply a freeze drier, but I am
} not
} } } sure. Does anyone out there have any insight into what this thing is or
} } } what it does? Thanks for your help!!
} } }
} } } Tony
} } }
} } }
} } } Tony Kowal
} } } Research Assistant
} } } for
} } } Dr. Susan Lindquist
} } }
} } } Howard Hughes Medical Institute
} } } The University of Chicago
} } } 5841 S. Maryland
} } } MC 1028, Room N339
} } } Chicago, IL 60637
} } }
} } } Phone: (773) 702-8795
} } } Fax: (773)702-7254
} } } e-mail: askowal-at-midway.uchicago.edu
} } } Pager: on campus - 188 - 9668 (YNOT)
} } } off campus - (773)753-1880 - 9668
} } }
} } }
} } }
} }
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Dr Chris Jeffree
} } University of Edinburgh
} } Biological Sciences EM Facility
} } Daniel Rutherford Building
} } King's Buildings EDINBURGH EH9 3JH
} } Tel: +44 (0) 131 650 5345
} } FAX: +44 (0) 131 650 6563
} }
} } Inveresk Cottage, 26 Carberry Road,
} } Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} } Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
}
} -------- REPLY, End of original message --------
}
}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Mon May 29 08:06:37 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 29 May 2000 21:41:45 +1000
Subject: RE: Tissue Drier/ or CPD

Contents Retrieved from Microscopy Listserver Archives
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When using published methodology, some things matter, others do not.
How to know which and what? I don't know, other then a good understanding of
the field.
Anyway, I have been amused over time with researchers insisting on outdated or
cumbersome methods, because "it was published".
In this particular case I would like to note that the tissue dryer is a freeze
dryer designed for microscopy samples. Several other such instruments would do
equally well and I expect that rather more researchers have prepared yeast by
the critical point method.
Freeze drying or critical point drying both work well for numerous samples.
There will be a few specimens that are better prepared by one means or the
other, but I wonder how frequently the claim "this gives better preservation"
should have the disclaimer "in my hands" added.

Point is that you want to dry the yeast for SEM and you may be wasting your
time chasing a particular instrument, when another, perhaps already in the
department would do equally well.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Sunday, May 28, 2000 3:39 AM, Tony Kowal [SMTP:askowal-at-midway.uchicago.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Fellow Microscopists -
} Someone in the lab in which I work wants to do SEM of yeast colonies. I
} was able to locate some references and a general protocol, which seems
} rather straight forward. However, the authors two of the papers refer to
} an "Edwards Pearce Tissue Drier, EPD3". I contacted Edwards to find out
} what this tissue drier is, however they said they could not give me any
} info because it is no longer manufactured.
} I am thinking that this tissue drier is simply a freeze drier, but I am not
} sure. Does anyone out there have any insight into what this thing is or
} what it does? Thanks for your help!!
}
} Tony
}
}
} Tony Kowal
} Research Assistant
} for
} Dr. Susan Lindquist
}
} Howard Hughes Medical Institute
} The University of Chicago
} 5841 S. Maryland
} MC 1028, Room N339
} Chicago, IL 60637
}
} Phone: (773) 702-8795
} Fax: (773)702-7254
} e-mail: askowal-at-midway.uchicago.edu
} Pager: on campus - 188 - 9668 (YNOT)
} off campus - (773)753-1880 - 9668
}
}



From daemon Mon May 29 19:15:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 29 May 2000 13:12:19 -0400
Subject: Re: charred plant seeds

Contents Retrieved from Microscopy Listserver Archives
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Paul,

Has she looked at them with conventional light microscopy, either stereo or
compound? Following Oxam's Razor, this approach seems to be the simplest
and should be tried first.

Best regards
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



At 04:19 PM 5/27/00 EDT, Pbgrover-at-aol.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue May 30 11:10:39 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 30 May 2000 09:13:06 -0500
Subject: charred plant seeds

Contents Retrieved from Microscopy Listserver Archives
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Paul,

It's been awhile since I was involved in this kind of research (i.e.,
graduate work in palaeoethnobotany, but I do not recall that seeds need to
be "charred", per se, to be preserved over large periods of time. I believe
that seeds and other plant remains can also become carbonized without fire,
as a result of oxidation, etc., in the depositional environment.

To detect charring in the SEM might be a real problem, especially if trying
to differentiate it from carbonization by means other than fire. I suppose
that "charred" seeds might show physical damage more than other seeds, but
even that might not always be true. The charging effects you observed, on
the other hand, might possibly be the effects of mineralization of the seeds
as the original seed components become replaced with non-conductive soil
constituents over time. If so, this might indicate that these seeds are
indeed quite old.

I guess if I was faced with this problem as an archaeologist I would try to
identify other indications of fire in the context in which the seeds were
found, such as wood charcoal, hearths, etc. On the EM side, if I had access
to WDS, I might try comparing amounts of carbon in the old seeds, versus
fresh ones.

Finally, the simplest thing to try might just be to toast some fresh seeds
and compare them to non-toasted ones of the same type. Try a couple
different charring methods, like throwing some in a campfire and collecting
them later, and toasting them on a frying pan. A reference you might look
at is "Palaeoethnobotany", a book by Dr. Deborah Pearsall published by
Academic Press. I think it just came out in a new edition.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Pbgrover-at-aol.com"-at-sparc5.microscopy.com]
Sent: Saturday, May 27, 2000 3:19 PM
To: Microscopy-at-sparc5.microscopy.com


Esteemed colleagues,

A student has asked me if I can find a way to tell whether small plant seeds

have been charred or not. She is examining seeds from an anthropological
dig
on a site probably inhabited both in historic times (170 years ago) and
prehistoric (1000+ years ago). It seems that for seeds to date from the
earlier inhabitation, they would need to be charred, which apparently
preserves them.

I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of
same
species as controls. The seeds of unknown age showed a lot more charging
than the fresh ones. Could this be evidence that they are uncharred (i.e.,
would charring make them more conductive/better secondary electron
producers?) Does anyone know of a method of differentiating between charred

& uncharred seeds?

Thank you thank you thank you :0)

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN



From daemon Tue May 30 15:41:05 2000



From: Peter Bond :      P.Bond-at-plymouth.ac.uk
Date: Tue, 30 May 2000 11:11:23 -0500
Subject: Film vs Digital

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Hi all

The continuing discussion on whether film is better than digital, and
whether we can directly compare their resolving ability is very interesting
and throwing up some really useful technical stuff. But aren't we missing
the point a bit? Surely the image quality is user defined - if you or your
customer, are satisfied with the end product then the equipment has done
its job. How often does one need to examine a micrograph to assess its
limits? If are getting that close to a picture then you may be taking
things out of context a bit and losing the whole concept.

The future is with digital image capture, let's hope the price of the
equipment comes down to something more attainable!

Pete

Peter Bond
Plymouth Electron Microscopy Unit
University of Plymouth
Drake Circus
Plymouth
Devon
UK PL4 8AA
Tel/Fax: 01752 233092
email: pbond-at-plymouth.ac.uk




From daemon Tue May 30 15:41:05 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Mon, 29 May 2000 22:49:54 +0100
Subject: BSE Images

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Hi

A discussion is developing from my comment that a de-saturated W hairpin
source provides a better BSE image. This "problem" first came to light when
a South African client commented that they have far better atomic number
contrast images if they run on "first peak" rather than gun saturation.

I based my explanation on some work by C-R Peters, he relates BSE and SE
signals to probe size. The bigger the probe the bigger the reaction volume
that is the source of the BSE contribution to an image.

In the de-saturated state a normal W hairpin system has a very much bigger
source which reflects in a very much larger probe being placed on the
specimen than would be obtained under a "normal saturation" situation and
therefore provides the operator with more BSE.

Some instruments do give a higher signal at their false peak than at what we
would know as saturation. I have seen this on Cambridge (Leica, Leo)
instruments and no matter how hard you try to "correct" what you feel is a
mis alignment you just do not win. On some occasions I have also seen this
on a Philips but it is much more rare.

I put this down to gun design as you do not see this in a Japanese designed
instrument. In their case a higher false peak indicates a definite mis
alignment!

I base my comments on running courses with a different SEM in a different
laboratory almost every week, not on what happens with one laboratory on one
instrument.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Tue May 30 15:41:05 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 30 May 2000 11:23:33 -0600 (MDT)
Subject: Epoxy in Path-Research Labs

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Laboratories who have to do pathology work and research also, have
particular problems in that they are faced with a wide variety of
specimens not all of which are suitable for a single epoxy formulation
mixture during embedment.

Many pathology laboratories in the US use the medium hard formulation by
Luft. This is indeed a good, nearly all purpose formulation, however, in
laboratories that have to use glass knives for thick sectioning the NMA
contained in this formulation deterioates the edge of the glass knife very
quickly. If glass knives have to be used for thin sectioning, then this
formulation cannot be used at all. Otherwise with diamond knives it cuts
very well, assuming of course, that the embedding has been done well.
The original formulation by Luft required two mixtures to be made up
seperately. Years ago I combined this into a single mixture which can be
easily frozen (without accelerator!) and kept for months at -80 or -20.

Eponate 12 148 ml
DDSA 100 ml
NMA 76 ml

This makes 324 ml. Accelerate it with 1.5% DMP-30 before use. If you
are interested in this formulation, please print it out now, because this
is the last time I will address it.

Sometimes laboratories are faced with processing floating cells. These
ar e best enrobed in agarose, hopefully after osmication. In order to
avoid enormous cell loss during spinning down repeatedly into embedding
medium, it is fortuitous to draw off all buffer after osmium, and spin the
cells into a mixture of 3% Ultra-low temperature agarose, Type IX (Sigma).
This agarose type gells at 15 deg C, and it has the property of not being
so dense and fibrous that embedding medium cannot penetrate it. After the
gel is cooled (in the refrigerator, or on ice), the blocks are cut of
desired size. It is important to note that one is no longer dealing with
cells, but actual blocks and the protocol for dehydration and embedding
must fit the requirements of the block size. The resulting blocks also
are very well embedded in the above formulation.

The above formulation is good for skin and muscle, but the protocol must
be adjusted to allow adequate penetration. We do 2 hours for dehydration,
use propylene oxide for an intermediate, and start infiltrating with
mixtures of PO and Epoxy, 2:1, 1:1, 1:3, pure, pure, pure, etc. When the
tissue goes into the newly accelerated pure mixtures, I put an ordinary
60W bulb in the vicinity so that the mixture can heat to about 37 deg. At
this point the formulation becomes very liquid and infiltration is
enhanced. After 1.5 hours the lamp is turned off as minor polymerization
begins about 40 deg C, which is undesirable during infiltration.

A problem arises when laboratories have to produce a large number of thick
sections with glass knives, or they have to also use glass knives for thin
sectioning. Then the above formulation cannot be used. A new embedding
medium containing no NMA is needed. Many years ago, Mollenhauer invented
a "Mixed Embedding" system which contained Araldite 502, Epon, DDSA, and
dibutyl pthalate. This is an extremely useful system - a joy to section
with glass knives. The downside is that it is more viscous than media
without Araldite, and it takes longer to infiltrate. Here is the
formulation, again reconfigured by me years ago into a single mixture.

Araldite 502 30ml
Eponate 12 50 ml
DDSA 55 ml
Dibutyl (EMS) 0.75% (mix in with the 3 items above
DMP-30 1.5% (add at time of use)

Freeze mixture without the accelerator. Will keep many months at -80. Can
also keep at -20. For thick sectioning I polymerize just 24 hours. If
blocks are to be thin sectioned, then I put them back into the oven for
another 24 or 48 hours. This formulation has the capability of being soft
and stiff, as you wish. If only thick sections are to be done, use 1%
dibutyl. If blocks are too soft for your liking, just put them into a 60
deg C oven for several days, or use 95 deg C for an hour or two. One time
I forgot some blocks in the 95 deg oven for a week. They still sectioned
well.

Again, anyone interested please print this out now. I will not be
answering these questions again, nor write these formulations again.

This is fun! Keep accurate records of what you do, particularly the
infiltration and dehydration times. Finally you will have every block a
joy to handle. No frustrations!

Bye,
Hildy Crowley
Sr. Electron Microscopy Specialist
University of Denver
Denver, CO

P.S. It is assumed that all processing is done with vials in constant
motion! Once infiltration starts, vials must be on a rotator, not on a
rocker or a shaker, as the monomers of the formulations will seperate and
poor embedding will result.



From daemon Tue May 30 15:41:07 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 30 May 2000 15:17:51 -0400
Subject: Re: Film vs Digital

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Peter Bond wrote:

} The continuing discussion on whether film is better than digital, and
} whether we can directly compare their resolving ability is very interesting
} and throwing up some really useful technical stuff. But aren't we missing
} the point a bit? Surely the image quality is user defined - if you or your
} customer, are satisfied with the end product then the equipment has done
} its job. How often does one need to examine a micrograph to assess its
} limits? If are getting that close to a picture then you may be taking
} things out of context a bit and losing the whole concept.
}
} The future is with digital image capture, let's hope the price of the
} equipment comes down to something more attainable!
}

Dear Pete,
If one is doing quantitative image processing, the better
resolution
available from film can be relevant. For example, if one wants to do
corellation averaging, one needs as many objects in the picture as possible,
and also as good resolution as needed--e.g., for 1 nm resolution of the
reconstruction, a pixel size of 0.25 nm times the magnification is necessary,
so the recording medium must have resolution equal to or better than that.
The pixel size of the scanner must also be this small--obviously irrelevant
for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one will have
about 12,000 by 16,000 (taking the header into account) pixels of useful
information. This will give a broader area than that available at equal
resolution from any CCD chip now out there. I can assure you that I have
often had to determine the information limits in a particular micrograph.
Yours,
Bill Tivol




From daemon Tue May 30 15:41:07 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 30 May 2000 15:21:25 -0400 (EDT)
Subject: curing Epon/Araldite with UV?

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Greetings!

Has anyone ever cured Epon-Araldite with UV? I was given a
cobbled-together protocol by a non-microscopist post-doc; the protocol
calls for a dry-ice, UV cure of Epon-Araldite. It says it can take from
3-7 days. I'm wondering if this is either a resin mix-up *or* the resin
just set up all by itself (over that time frame I've seen it happen), just
because it was a thin layer and left alone - nothing to do with the UV
exposure. She has no ref. for this technique and isn't sure now where she
got it. Sigh. I've only been able to find heat-cure protocols...anyone
have any leads or thoughts on this UV thing?

(Sorry about the cross-post for those of you on both of these servers)

Thanks!

Tamara (Planning to use the oven.....) Howard
CSHL



From daemon Tue May 30 17:42:26 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 30 May 2000 15:28:04 -0700
Subject: RE: BSE Images

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Steve Chapman writes ...

} ...
}
} I based my explanation on some work by C-R Peters, he
} relates BSE and SE signals to probe size.
} The bigger the probe the bigger the reaction volume
} that is the source of the BSE contribution to an image.
}
} In the de-saturated state a normal W hairpin system has a
} very much bigger source which reflects in a very much
} larger probe being placed on the specimen than would be
} obtained under a "normal saturation" situation and
} therefore provides the operator with more BSE.


I have seen the 1st peak provide more beam current, but I fail to see
how a larger probe diameter at similar beam currents can provide
better z contrast. Do you know what the beam current is ... "1st
peak" vs "saturated"??

I might also suggest the 1st peak may provide emission from several
areas of the filament instead of just from the tip. This would
manifest as "ghost" images or double images ... leastwise, I hope this
type of phenomenon couldn't be confused with "better z contrast" :o)

=shAf=



From daemon Tue May 30 21:10:39 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 31 May 2000 12:17:58 GMT+1200
Subject: Stage drive motors for 840A

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Hi

Does anyone know of anybody who sells motors with suitable mechanical
interfaces to drive X and Y of a JEOL 840A stage?

Replies from suppliers very welcome.

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue May 30 21:10:40 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 31 May 2000 12:51:08 GMT+1200
Subject: PCD for 840

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Hi again

This is my last posting, I promise, looking for a Probe Current
Detector (PCD) for a JEOL 840.
The pneumatically-powered Faraday Cup which shoots into the beam just
below the objective aperture, samples the beam, and retracts.

Does anyone have one which is redundant or otherwise spare?

Alternatively, does anyone know of a third-party manufacturer of this
sort of thing?

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue May 30 21:10:40 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 30 May 2000 18:05:47 -0700
Subject: Re: Film vs Digital

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At 12:17 PM 5/30/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The best CCD imagers are typically 6u in size. Complicating this
is that they may be square or rectangular. Fuji's new hex-shaped
pixels may be a major improvement. We'll see.

gg



From daemon Wed May 31 07:11:30 2000



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Wed, 31 May 2000 12:13:17 +1000
Subject: Our civil engineering department is trying to look at the

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Our civil engineering department is trying to look at the bonding
between bitumen and aggregate with the aid of a SEM. However, we do not
have ESEM facilities (as I suggested). Now I was ask to see if anyone in
the EM fraternity has any experience in using standard SEMs for
examining bitumen containing specimens (I have not!!; and I don't want
to ruin my microscopes).The supplied samples have been cut into slices
of approximately 4mm thickness.

Any ideas out there?
Thanks



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Wed May 31 07:11:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 31 May 2000 11:13:10 +1000
Subject: RE: Film vs Digital

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Peter Bond is right in saying "The future is with digital image capture" if we
were at some future date to count heads of digital versus film TEM users.
That change will be for reasons of convenience and to save labour. These are
powerful and valid reasons.

Bill Tivol has given one set of applications where digital currently does not
always measure up to film.
Here are another couple of such applications.
1 When great enlargements are required film is superior. This is because of
film's greater resolution, but more importantly, because its much, much easier
to take images at moderate powers and highly enlarge. That way we take
advantage of the TEM's greater depths of focus at low powers and of film's
higher resolution/ image detail.
2 Whenever a TEM image is taken at low brightness (to avoid beam damage or at
very high powers or in dark-field) relatively few electrons form the image and
make that image grainy. Film is very slow and requires then a longer exposure,
thus boosting the quantity of electrons used and improving the image. Digital
is much more sensitive and so the exposure must be shortened. As a result the
best digital camera will record, quiet faithfully the grainy, unsharp image.

Many labs rarely or never use such applications and for them the reasons to
change to digital now may be overwhelming.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, May 31, 2000 5:18 AM, William Tivol [SMTP:tivol-at-wadsworth.org]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Peter Bond wrote:
}
} } The continuing discussion on whether film is better than digital, and
} } whether we can directly compare their resolving ability is very interesting
} } and throwing up some really useful technical stuff. But aren't we missing
} } the point a bit? Surely the image quality is user defined - if you or your
} } customer, are satisfied with the end product then the equipment has done
} } its job. How often does one need to examine a micrograph to assess its
} } limits? If are getting that close to a picture then you may be taking
} } things out of context a bit and losing the whole concept.
} }
} } The future is with digital image capture, let's hope the price of the
} } equipment comes down to something more attainable!
} }
}
} Dear Pete,
} If one is doing quantitative image processing, the better
} resolution
} available from film can be relevant. For example, if one wants to do
} corellation averaging, one needs as many objects in the picture as possible,
} and also as good resolution as needed--e.g., for 1 nm resolution of the
} reconstruction, a pixel size of 0.25 nm times the magnification is necessary,
} so the recording medium must have resolution equal to or better than that.
} The pixel size of the scanner must also be this small--obviously irrelevant
} for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
} gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one will have
} about 12,000 by 16,000 (taking the header into account) pixels of useful
} information. This will give a broader area than that available at equal
} resolution from any CCD chip now out there. I can assure you that I have
} often had to determine the information limits in a particular micrograph.
} Yours,
} Bill Tivol
}
}



From daemon Wed May 31 07:11:33 2000



From: gao-at-nirim.go.jp (Gao Yihua)
Date: Wed, 31 May 2000 14:09:13 +0900 (JST)
Subject: Could someone help me to solve the question

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Dear Colleagues:

I have a question about the structure of 2H-SiC (x-ray card
No.29-1130), which belong to No. 186 space group (P63mc). (a=0.3076 nm,
c=0.5048 nm, alfa=beta=120 degrees, gama=90 degrees)

My question is as below:

(A) Based on the symmetry of space group No. 186, the coordinates of
the atoms in a unit cell should be

Si 2a : (0,0,0), and (0,0,0.5)

C 2b : (2/3, 1/3, 0.125) and (1/3,2/3,0.625)


(B) However, acoording to the real stacking sequence, the
coordinates of the Si and C atoms in a unit cell are

Si sites: (0, 0, 0) and (2/3, 1/3, 0.5)
C sites: (2/3, 1/3, 0.125) and (0, 0, 0.625)


It seems that there are some conflict between the two sets of
coordinates, could someone help me to solve such a problem.


Thank you vert much

Yours sincerely
Gao Yihua






From daemon Wed May 31 07:11:34 2000



From: Dennis Ward :      DCWard-at-concentric.net
Date: Wed, 31 May 2000 04:37:07 -0700
Subject: EMSA format

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Could someone direct me to a reference that describes the EMSA formats for
spectra?
Thanks.

Dennis.

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net



From daemon Wed May 31 07:11:36 2000



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Wed, 31 May 2000 12:37:47 +0100 (BST)
Subject: Re: Stage drive motors for 840A

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 31 May 2000, Ritchie Sims wrote:

} Does anyone know of anybody who sells motors with suitable mechanical
} interfaces to drive X and Y of a JEOL 840A stage?
}
} Replies from suppliers very welcome.

Ritchie,

Deben UK Ltd, 11-15 High St, Stowmarket, Suffolk UK IP14 6QL make very
nice motors and controllers for X, Y and Z control of SEM stages. You can
also contact them at : info-at-deben.co.uk or via their webpage :
http://www.deben.co.uk

Cheers,

David Vowles
Electron Microscopy Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-msm.cam.ac.uk




From daemon Wed May 31 07:11:36 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Wed, 31 May 2000 13:41:17 +0200
Subject: Atom locations with P6_3mc

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Dear Gao,
we have used simulation software for CBED for both ZnO and GaN which are
isomorphic with 2H-SiC (same space group:P6_3mc). For our software we use
the following atom locations (I will use Si & C instead):

Si positions: (1/3, 2/3, 0) and (2/3, 1/3, 1/2)

C positions: (1/3, 2/3, u) and (2/3, 1/3, u + 1/2)

The Si-C bond length is parameterized by the quantity u. For the ideal
(perfect stacking) arrangement u=3/8. However I am reliably informed that
for SiC the u parameter is a great mystery at the present. However using
u=3/8 should prove to be a good starting point.

Please note that to place Si at (0,0,0) you will have to remove (1/3,2/3,0)
from each coordinate above which gives the following locations:

Si: (0,0,0) and (1/3, 2/3, 1/2)

C: (0,0,u) and (1/3, 2/3, u + 1/2)

Does this make sense?

Regards,
Jonathan
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Wed May 31 07:21:42 2000



From: DAVID I SAXON :      DISAXON-at-prodigy.net
Date: Wed, 31 May 2000 07:19:59 -0500
Subject: roughness of polyethylene

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Dear List,
I'm looking for methods for measuring the roughness of a sheet of
polyethylene. The film consists of grains (80 to 150 microns in diameter)
which are loosely packed. Large area analysis is prefered since the surface
is very irregular locally.

TIA for any suggestions,
David Saxon




From daemon Wed May 31 09:50:24 2000



From: HDMHOS-at-aol.com
Date: Wed, 31 May 2000 09:31:38 EDT
Subject: cryo epoxy

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Hello everybody:
I will like to do cryomicrotomy of PP wires. My question is : can anyone
suggest what kind of epoxy is appropriate for cryomicrotomy?
I used Epoxy Mount and it was chattered during cryo process. The
manufacturer confirm to me that epoxy mount is not appropriate for
cryomicrotomy. Can anynone help me?

Thank You

Briget
Email: HDMHOS-at-AOL.COM



From daemon Wed May 31 09:50:24 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 31 May 2000 08:42:51 -0400
Subject: Re: Epoxy in Path-Research Labs

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HI Hildy,
You are always a great source of information....thanks.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed May 31 09:50:25 2000



From: wft03-at-health.state.ny.us
Date: Wed, 31 May 2000 10:27:45 -0400
Subject: RE: Film vs Digital

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2 Whenever a TEM image is taken at low brightness (to avoid beam damage
or at
very high powers or in dark-field) relatively few electrons form the image
and
make that image grainy. Film is very slow and requires then a longer
exposure,
thus boosting the quantity of electrons used and improving the image.
Digital
is much more sensitive and so the exposure must be shortened. As a result
the
best digital camera will record, quiet faithfully the grainy, unsharp
image.


Dear Jim,

Your points are good; however, exposure times are not, by

themselves, relevant. An exception to this is if the damage depends

on the dose rate; i.e., if there are mechanisms to dissipate the absorbed

beam energy which limit damage at low illumination levels. The

image quality depends on how much information is carried by each elec-

tron (basically, the number of photons produced in the scintillator times

the quantum efficiency of the system for these photons for digital, and the

probability of a darkened film grain for film or image plates) times the

number of electrons. The damage--except for dose-rate-dependence--

is linear with the number of electrons, so it is the efficiency of the pro-

cess of obtaining the information from each electron which determines

the ultimate resolution limit. Also, the use of image averaging can make

one clear image from many grainy ones in the case that one has a large

number of nominally identical specimens.

Yours,

Bill




From daemon Wed May 31 10:19:45 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 31 May 2000 09:49:38 -0500
Subject: RE: charred plant seeds

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Randy,
Don't forget about other analytical techniques, such as XPS. Some well
engineered surface analysis may reveal significant chemical differences
between "charred", new, and aged or mineralized specimens. In addition to
Randy's good advice on the microscopy, I would also utilize the power of
both TEM and EELS in comparing morphology and carbon and oxygen chemistry.
Electron energy loss spectroscopy combined with EDS is one of my favorites
for carbon micro-analyses. Looking at both the low loss and the core loss
structure of EELS spectra, there is tremendous detail and differentiation to
be obtained. If TEM and "PEELS" are available, and you don't mind
sacrificing a bit of the artifact, a series of control specimens and
analysis by "AEM" (TEM, EDS, and PEELS) would be worth a shot.
Sounds like fun, Good Luck,
Brad Huggins
BP Amoco, Analytical
Naperville IL

} ----------
} From: Tindall, Randy D.[SMTP:TindallR-at-missouri.edu]
} Sent: Tuesday, May 30, 2000 9:13 AM
} To: '"Pbgrover-at-aol.com"-at-sparc5.microscopy.com'
} Cc: 'microscopy-at-sparc5.microscopy.com'
} Subject: RE: charred plant seeds
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Paul,
}
} It's been awhile since I was involved in this kind of research (i.e.,
} graduate work in palaeoethnobotany, but I do not recall that seeds need to
} be "charred", per se, to be preserved over large periods of time. I
} believe
} that seeds and other plant remains can also become carbonized without
} fire,
} as a result of oxidation, etc., in the depositional environment.
}
} To detect charring in the SEM might be a real problem, especially if
} trying
} to differentiate it from carbonization by means other than fire. I
} suppose
} that "charred" seeds might show physical damage more than other seeds, but
} even that might not always be true. The charging effects you observed, on
} the other hand, might possibly be the effects of mineralization of the
} seeds
} as the original seed components become replaced with non-conductive soil
} constituents over time. If so, this might indicate that these seeds are
} indeed quite old.
}
} I guess if I was faced with this problem as an archaeologist I would try
} to
} identify other indications of fire in the context in which the seeds were
} found, such as wood charcoal, hearths, etc. On the EM side, if I had
} access
} to WDS, I might try comparing amounts of carbon in the old seeds, versus
} fresh ones.
}
} Finally, the simplest thing to try might just be to toast some fresh seeds
} and compare them to non-toasted ones of the same type. Try a couple
} different charring methods, like throwing some in a campfire and
} collecting
} them later, and toasting them on a frying pan. A reference you might look
} at is "Palaeoethnobotany", a book by Dr. Deborah Pearsall published by
} Academic Press. I think it just came out in a new edition.
}
} Good luck.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}
} -----Original Message-----
} } From: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Pbgrover-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Saturday, May 27, 2000 3:19 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: charred plant seeds
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Esteemed colleagues,
}
} A student has asked me if I can find a way to tell whether small plant
} seeds
}
} have been charred or not. She is examining seeds from an anthropological
} dig
} on a site probably inhabited both in historic times (170 years ago) and
} prehistoric (1000+ years ago). It seems that for seeds to date from the
} earlier inhabitation, they would need to be charred, which apparently
} preserves them.
}
} I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of
} same
} species as controls. The seeds of unknown age showed a lot more charging
}
} than the fresh ones. Could this be evidence that they are uncharred
} (i.e.,
} would charring make them more conductive/better secondary electron
} producers?) Does anyone know of a method of differentiating between
} charred
}
} & uncharred seeds?
}
} Thank you thank you thank you :0)
}
} Paul Grover
} Chief Microscopist and Bottle Washer
} Microvista Laboratory
} Lafayette, IN
}



From daemon Wed May 31 18:47:29 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 31 May 2000 09:31:24 -0500
Subject: RE: charred plant seeds

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Could it be that moisture escaping from the fresh seeds somehow helps to
dissipate the charge so that new seeds do not charge like the old ones?

I would also be interested in the x-ray spectra of the two seeds. Perhaps
there is partial mineralization as Randy Tindall suggested. A light element
detector should also reveal a difference in O/C ratio due to differences in
moisture content, or perhaps due to charring.

Warren S.

At 09:13 AM 5/30/2000 -0500, you wrote:
} Esteemed colleagues,
}
} A student has asked me if I can find a way to tell whether small plant seeds
}
} have been charred or not. She is examining seeds from an anthropological
} dig
} on a site probably inhabited both in historic times (170 years ago) and
} prehistoric (1000+ years ago). It seems that for seeds to date from the
} earlier inhabitation, they would need to be charred, which apparently
} preserves them.
}
} I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of
} same
} species as controls. The seeds of unknown age showed a lot more charging
} than the fresh ones. Could this be evidence that they are uncharred (i.e.,
} would charring make them more conductive/better secondary electron
} producers?) Does anyone know of a method of differentiating between charred
}
} & uncharred seeds?
}
} Thank you thank you thank you :0)
}
} Paul Grover
} Chief Microscopist and Bottle Washer
} Microvista Laboratory
} Lafayette, IN



From daemon Wed May 31 18:47:29 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 31 May 2000 09:17:51 -0600
Subject: Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Of course there are situations where one or the other Technique may be
better because of the limitations of film or digital imaging. And
especially for a task like the one Bill talks about below, where field
of view AND resolution are important, images taken on film may be
superior to images from a digital camera. On the other hand, digital
imaging may have something to offer in those cases as well:

Instead of taking one image and do the corellation averaging, why not
have the computer do it? I could probably set up a system that acquires
the image at a high enough resolution, extract all the necessary data,
then move the stage to an adjacent area and continue the measurements
there. This technique would even have an advantage over film: I can set
the lower limits for the accuracy before taking the images and the
system continues to measure until these limits are satisfied. This could
be done without user intervention. So, instead of taking one image,
scanning it in with a scanner, and then being "limited" by the field of
view to a certain measurement accuracy, one could start the measurement
with a predetermined accuracy, go drink a coffee, work on that paper, do
the travel expense report, then go back to the microscope and collect
the spreadsheet with the measurement data.
Of course this requires the setup of a fairly complex system, but those
are technical problems, not fundamental ones.

Just a thought ....


Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: William Tivol [mailto:tivol-at-wadsworth.org]
Sent: Tuesday, May 30, 2000 1:18 PM
To: microscopy-at-sparc5.microscopy.com


Peter Bond wrote:

} The continuing discussion on whether film is better than digital, and
} whether we can directly compare their resolving ability is very
interesting
} and throwing up some really useful technical stuff. But aren't we
missing
} the point a bit? Surely the image quality is user defined - if you or
your
} customer, are satisfied with the end product then the equipment has
done
} its job. How often does one need to examine a micrograph to assess its
} limits? If are getting that close to a picture then you may be taking
} things out of context a bit and losing the whole concept.
}
} The future is with digital image capture, let's hope the price of the
} equipment comes down to something more attainable!
}

Dear Pete,
If one is doing quantitative image processing, the better
resolution
available from film can be relevant. For example, if one wants to do
corellation averaging, one needs as many objects in the picture as
possible,
and also as good resolution as needed--e.g., for 1 nm resolution of the
reconstruction, a pixel size of 0.25 nm times the magnification is
necessary,
so the recording medium must have resolution equal to or better than
that.
The pixel size of the scanner must also be this small--obviously
irrelevant
for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one will
have
about 12,000 by 16,000 (taking the header into account) pixels of useful
information. This will give a broader area than that available at equal
resolution from any CCD chip now out there. I can assure you that I
have
often had to determine the information limits in a particular
micrograph.
Yours,
Bill Tivol




From daemon Wed May 31 18:47:30 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 31 May 2000 09:42:40 -0600
Subject: RE: Film vs Digital

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Hello Jim,

just a couple of remarks to your email.

your remark 1) What you can do of course with digital imaging (provided
you have the necessary hardware), is to automatically collect larger
areas by taking several images that overlap, reducing the advantage that
film has in this area and perhaps offer other possibilities as I just
explained in another posting as a response to Bill Tivol's posting.

your remark 2) I am not sure you are not comparing apples and oranges.
What you are saying is, that because of the "slowness" of film you need
longer exposures, thereby averaging out the statistical noise of the
electron, which is not the case for CCD cameras at short exposures,
hence they appear more noisy. In essence what you are doing is to
compare a short exposure image to a long exposure image. What you can do
with a CCD camera is the following: You can get (perhaps) real-time dark
field images and position your sample and/or decide if you want to
actually take the image. Then you take a SERIES of images, let's say 10,
each at an exposure time of 1/10 of the film exposure. This can be done
automatically, of course. Finally, you add or average all of these
images using a pattern recognition to align them first. The result: A
dark field image that should be as noisy as the film image, with much
less problems due to drift during long exposures, a higher dynamic range
and visible immediately on the viewing screen.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Tuesday, May 30, 2000 7:13 PM
To: 'William Tivol'
Cc: microscopy-at-sparc5.microscopy.com


Peter Bond is right in saying "The future is with digital image capture"
if we
were at some future date to count heads of digital versus film TEM
users.
That change will be for reasons of convenience and to save labour. These
are
powerful and valid reasons.

Bill Tivol has given one set of applications where digital currently
does not
always measure up to film.
Here are another couple of such applications.
1 When great enlargements are required film is superior. This is
because of
film's greater resolution, but more importantly, because its much, much
easier
to take images at moderate powers and highly enlarge. That way we take
advantage of the TEM's greater depths of focus at low powers and of
film's
higher resolution/ image detail.
2 Whenever a TEM image is taken at low brightness (to avoid beam
damage or at
very high powers or in dark-field) relatively few electrons form the
image and
make that image grainy. Film is very slow and requires then a longer
exposure,
thus boosting the quantity of electrons used and improving the image.
Digital
is much more sensitive and so the exposure must be shortened. As a
result the
best digital camera will record, quiet faithfully the grainy, unsharp
image.

Many labs rarely or never use such applications and for them the reasons
to
change to digital now may be overwhelming.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, May 31, 2000 5:18 AM, William Tivol
[SMTP:tivol-at-wadsworth.org]
wrote:
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Peter Bond wrote:
}
} } The continuing discussion on whether film is better than digital,
and
} } whether we can directly compare their resolving ability is very
interesting
} } and throwing up some really useful technical stuff. But aren't we
missing
} } the point a bit? Surely the image quality is user defined - if you
or your
} } customer, are satisfied with the end product then the equipment has
done
} } its job. How often does one need to examine a micrograph to assess
its
} } limits? If are getting that close to a picture then you may be
taking
} } things out of context a bit and losing the whole concept.
} }
} } The future is with digital image capture, let's hope the price of
the
} } equipment comes down to something more attainable!
} }
}
} Dear Pete,
} If one is doing quantitative image processing, the better
} resolution
} available from film can be relevant. For example, if one wants to do
} corellation averaging, one needs as many objects in the picture as
possible,
} and also as good resolution as needed--e.g., for 1 nm resolution of
the
} reconstruction, a pixel size of 0.25 nm times the magnification is
necessary,
} so the recording medium must have resolution equal to or better than
that.
} The pixel size of the scanner must also be this small--obviously
irrelevant
} for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
} gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one
will have
} about 12,000 by 16,000 (taking the header into account) pixels of
useful
} information. This will give a broader area than that available at
equal
} resolution from any CCD chip now out there. I can assure you that I
have
} often had to determine the information limits in a particular
micrograph.
} Yours,
} Bill Tivol
}
}



From daemon Wed May 31 18:47:30 2000



From: Michal Jarnik :      M_Jarnik-at-fccc.edu
Date: Wed, 31 May 2000 11:57:49 -0500
Subject: Job Opening

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Job Opening


Electron Microscopy Facility at Fox Chase Cancer Center is seeking a
motivated individual for a Technician/Research Assistant position.

Required qualifications: B.S. or M.S. in biology, 2+ years of experience
in biological electron microscopy.

Responsibilities include sample preparation and electron microscopy
(TEM/SEM), dark room work, computer image processing, report
preparation. The great variability of work due to collaborations with
large number of laboratories provides exceptional opportunity for
professional growth.

We offer a competitive salary commensurate with experience, an excellent
benefit package (health/dental insurance, pension plan, paid vacation)
and a very friendly working environment.

For confidential consideration, please send a CV including a statement
of experience to:

Dr. Michael Jarnik
Fox Chase Cancer Center
EM Facility
7701 Burholme Avenue
Philadelphia, PA 19111
e-mail: m_jarnik-at-fccc.edu



From daemon Wed May 31 18:47:31 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Wed, 31 May 2000 12:58:30 -0400
Subject: Graduate Students needed at UCF

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MS level and PhD level graduate students are needed for research/education
in the Materials Science and Engineering Discipline at the University of
Central Florida.

Students will also work closely with Cirent Semiconductor (Lucent
Technologies, Orlando, FL) staff scientists.

For more information please contact:

Dr. Lucille A. Giannuzzi
Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826
email lag-at-mail.ucf.edu
phone (407) 275-4354,5,6
fax (407) 275-4321

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Wed May 31 18:47:33 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 31 May 2000 13:58:52 -0400
Subject: Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Mike Bode wrote:

} Instead of taking one image and do the corellation averaging, why not
} have the computer do it? I could probably set up a system that acquires
} the image at a high enough resolution, extract all the necessary data,
} then move the stage to an adjacent area and continue the measurements
} there. This technique would even have an advantage over film: I can set
} the lower limits for the accuracy before taking the images and the
} system continues to measure until these limits are satisfied. This could
} be done without user intervention. So, instead of taking one image,
} scanning it in with a scanner, and then being "limited" by the field of
} view to a certain measurement accuracy, one could start the measurement
} with a predetermined accuracy, go drink a coffee, work on that paper, do
} the travel expense report, then go back to the microscope and collect
} the spreadsheet with the measurement data.
} Of course this requires the setup of a fairly complex system, but those
} are technical problems, not fundamental ones.
}

Dear Mike,
That is an excellent suggestion. Of course, the beam should be

restricted to the area of the detector to avoid damaging the specimen
except where inevitable. You are correct that setting the accuracy
limits prior to taking the image avoids mixing the required data with
those from excess exposure, when the specimen has undergone some
radiation damage--this could perhaps be done with film, but it is
simpler with electronic data collection. There one can even make
sure that those pixels which are most important are the ones optimized,
and, with a small enough beam, like that used in spot-scan imaging,
one could expose different parts of the image for different times, so
that as much as possible of the image is optimally collected. This also
avoids damaging the area of the specimen not yet under investigation.

}
} Just a thought ....
}

And a good one.
Yours,
Bill Tivol



From daemon Wed May 31 18:47:33 2000



From: Jim Ferreira :      ferreira1-at-llnl.gov
Date: Wed, 31 May 2000 11:33:47 -0700
Subject: SEM Stereo Image Analysis Software

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Hello folks


Anyone aware of software capable of generating depth profiles from digital SEM stereo pair images? I am aware of the Oxford ISIS system/program, but wondered if other companies offer similar software that would run 'stand-alone' in a PC and work with any digital files.


Any help or suggestions along these lines would be most appreciated.


Thanks,

Jim




____________________________________________


{bold} {bigger} Scanning Electron Microscopy

{/bigger} {/bold} Jim Ferreira

Material Science & Technology Division

Chemistry & Material Science Directorate

Lawrence Livermore National Laboratory

PO Box 808 L-356, Livermore CA 94550

Ph: 925/424-4470 E-mail: ferreira1-at-llnl.gov



{/x-rich}



From daemon Wed May 31 18:47:34 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 31 May 2000 12:35:01 -0600 (MDT)
Subject: Re: curing Epon/Araldite with UV?

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On Tue, 30 May 2000, Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Greetings!
}
} Has anyone ever cured Epon-Araldite with UV? I was given a
} cobbled-together protocol by a non-microscopist post-doc; the protocol
} calls for a dry-ice, UV cure of Epon-Araldite. It says it can take from
} 3-7 days. I'm wondering if this is either a resin mix-up *or* the resin
} just set up all by itself (over that time frame I've seen it happen), just
} because it was a thin layer and left alone - nothing to do with the UV
} exposure. She has no ref. for this technique and isn't sure now where she
} got it. Sigh. I've only been able to find heat-cure protocols...anyone
} have any leads or thoughts on this UV thing?
}
} (Sorry about the cross-post for those of you on both of these servers)
}
} Thanks!
}
} Tamara (Planning to use the oven.....) Howard
} CSHL
}
}
}
Hi,

Try it, but not on anything valuable! Resins set up by themselves at rt.
I don't remember ever reading anything about UV in the Handbook of Epoxy
Resins. Why do you want to do it? That is the question. What would be
the advantage of that major fiddle?

Hildy Crowley



From daemon Wed May 31 18:47:34 2000



From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Wed, 31 May 2000 14:41:42 -0400
Subject: service

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We need service on our Balzer 500 freeze fractrure unit,
and was wondering who services them. Anyone we can contact?

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu



From daemon Wed May 31 18:47:35 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 31 May 2000 15:33:16 -0400
Subject: Re: Our civil engineering department is trying to look at

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Hans,

When I worked for Zeiss, we did a lot of coal analysis using
microspectrophotometry. I've also done some polarized light work on a
sample or two for customers. Is the bonding you are looking for on the
level where light microscopy might work? Just for your information, I also
recently took a look at the "tie" layers between polymer films in ketsup
bottles, perhaps a distant but related application. Polarized light and
DIC did a great job in that instance.

.. just a thought, but hopefully helpful.

Best regards,
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^




At 12:13 PM 5/31/00 +1000, Hans Brinkies wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed May 31 18:47:35 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 31 May 2000 15:35:07 EDT
Subject: Re: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you considered structured lighting? A flat beam of light is illuminates
the subject from a 45 degree angle. A camera is views it at 90 degrees.
The height and size of the roughness can be reconstructed from the shadows
the light makes.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "DAVID I SAXON" {DISAXON-at-prodigy.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 31, 2000 7:19 AM



In a message dated 5/31/00 2:52:24 PM, ferreira1-at-llnl.gov writes:

} Anyone aware of software capable of generating depth profiles from digital
} SEM stereo pair images? I am aware of the Oxford ISIS system/program,
} but wondered if other companies offer similar software that would run
'stand-alone'
} in a PC and work with any digital files.

One of the (many) functions in Fovea Pro (http://members.aol.com/FoveaPro), a
set of Photoshop-compatible plug-ins intended for the analysis of images
including those from microscopes such as SEMs, is a routine that fuses stereo
pair images to obtain the elevation of points on the surface. This can then
be used to measure elevation profiles, or to reconstruct 3D surface images.
Examples of both are included in the tutorial and are illustrated on the web
site. It isn't stand-alone (you need Photoshop or a compatible program), but
it will do what you ask.




From daemon Wed May 31 18:47:42 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Wed, 31 May 2000 17:51:23 -0500
Subject: tissue drier

Contents Retrieved from Microscopy Listserver Archives
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The tissue drier you referred to had a "cold finger" which acted as a vapor
trap to remove the water vapor produced from the sublimation of the specimen
ice. This is a very important feature for achieving "distortion-free drying"
which common type of freeze driers lack I believe.
Check out Pearse's book Histochemistry Theoretical and Applied, vol.1. It
contains an excellent chapter on this technique and others. However, I would
try CPD, also.

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Thu Jun 01 07:11:17 2000



From: P.Wang :      P.Wang-at-sheffield.ac.uk
Date: Wed, 31 May 2000 18:48:09 -0500
Subject: Turbo pump

Contents Retrieved from Microscopy Listserver Archives
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Hello, there,
Does anyone out here know that can we put alkylamine to the turbo pump
when carbon supporting film is being glow discharge?
Thanks.

Peiyi

Krebs Institute for Biomolecular Research
University of Sheffield
Firth Court
Western Bank
Sheffield
Yorkshire S10 2UH
United Kingdom
Tel: +44 (0)114 222 2000
Direct: +44 (0)114 222 2739
FAX: +44 (0)114 272 8697
E-mail: p.wang-at-sheffield.ac.uk




From daemon Thu Jun 01 07:11:18 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 31 May 2000 20:17:17 -0500
Subject: PP (coated) wires

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Briget wrote:
===========================================================
I will like to do cryomicrotomy of PP wires. My question is : can anyone
suggest what kind of epoxy is appropriate for cryomicrotomy? I used Epoxy
Mount and it was chattered during cryo process. The manufacturer confirm
to me that epoxy mount is not appropriate for cryomicrotomy. Can anynone
help me?
===========================================================
I am assuming you mean polypropylene coated wires and not polypropylene
monofilament. We have generally found that for coated wires samples, we
like to Pt coat it first (by sputtering), then embed in SPI-Pon™ 812 resin.
I would expect that any of the other "Epon® 812 substitutes", available
from the other major EM supplies firms would work just as well.

You will definitely want to use a diamond knife on this and you can vary the
hardness of the resin in way that gives you the best sections. Use a knife
angle that is not larger than 45°, the lower the temperature (usually) the
better.

Disclaimer: SPI Supplies offers for sale the resin and diamond knives
mentioned and performs this kind of cryoultramicrotomy for clients.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
===========================================



From daemon Thu Jun 01 07:11:18 2000



From: Shane Collins :      kshanec-at-gte.net
Date: Wed, 31 May 2000 17:15:18 -0700
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hamamatsu recently introduced a new camera called the electron bombardment
ccd where accelerated electrons directly bombard a back thinned, peltier
cooled ccd. The electrons are emitted from a photocathode for applications
particularly in low light microscopy. With a full well capacity of 300,000
electrons, I wonder if this approach could be used in direct exposure of the
ccd to the electron beam.
Shane

K. Shane Collins
Scientific Instrument Company
805.444.4953 cell
310.568.9188 office
310.568.9189 fax


-----Original Message-----
} From: Paul Voyles [mailto:voyles-at-research.nj.nec.com]
Sent: Friday, May 26, 2000 9:11 AM
To: Microscopy-at-sparc5.microscopy.com


} You could probably expose your CCD chip directly to the electron bean --
} and buy a new chip every few hundred exposures or so! Electrons have

There is an even worse problem with exposing the CCD directly the
electron beam. The p-n junctions in the CCD chip have a certain
"well capacity" - a number of electron/hole pairs they can hold
before they saturate. Fast (keV) electrons are much more efficient
at producing electron/hole pairs than photons - so much so that a
pixel on the CCD would saturate after about 15 fast electrons hit it.
Just the square root N shot noise at that level is about 25% - larger
than typical TEM micrograph contrast of 10-20%. That's why a
phosphor or scintillator is necessary to transform the fast electrons
into photons.




Happy reading,
Paul




From daemon Thu Jun 01 07:11:20 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 1 Jun 2000 13:36:52 +1000
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Yes, it could be done "in theory". Somebody would need to figure out the
software and perhaps modify the hardware. Then we would find that the total
exposure of the specimen to the electron beam maybe a muliple of the film's
exposure. Afterall, an 8 sec film exposure would not amount in digital to
10x0.8, but we would require considerable time in between exposures. Since the
problems in the discussed circumstances are specimen movement and beam damage,
it seems that taking multiple exposures is a poor option.

Digital cameras are for some situation too sensitive to electron exposure.
Cutting back on electrons is no option since its the electrons that form the
image in the first instance.
Much easier in light microscopy . . . insert a neutral density filter.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com


} Hello Jim,
}
} just a couple of remarks to your email.
}
} your remark 1) What you can do of course with digital imaging (provided
} you have the necessary hardware), is to automatically collect larger
} areas by taking several images that overlap, reducing the advantage that
} film has in this area and perhaps offer other possibilities as I just
} explained in another posting as a response to Bill Tivol's posting.
}
} your remark 2) I am not sure you are not comparing apples and oranges.
} What you are saying is, that because of the "slowness" of film you need
} longer exposures, thereby averaging out the statistical noise of the
} electron, which is not the case for CCD cameras at short exposures,
} hence they appear more noisy. In essence what you are doing is to
} compare a short exposure image to a long exposure image. What you can do
} with a CCD camera is the following: You can get (perhaps) real-time dark
} field images and position your sample and/or decide if you want to
} actually take the image. Then you take a SERIES of images, let's say 10,
} each at an exposure time of 1/10 of the film exposure. This can be done
} automatically, of course. Finally, you add or average all of these
} images using a pattern recognition to align them first. The result: A
} dark field image that should be as noisy as the film image, with much
} less problems due to drift during long exposures, a higher dynamic range
} and visible immediately on the viewing screen.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Tuesday, May 30, 2000 7:13 PM
} To: 'William Tivol'
} Cc: microscopy-at-sparc5.microscopy.com
} Subject: RE: Film vs Digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Peter Bond is right in saying "The future is with digital image capture"
} if we
} were at some future date to count heads of digital versus film TEM
} users.
} That change will be for reasons of convenience and to save labour. These
} are
} powerful and valid reasons.
}
} Bill Tivol has given one set of applications where digital currently
} does not
} always measure up to film.
} Here are another couple of such applications.
} 1 When great enlargements are required film is superior. This is
} because of
} film's greater resolution, but more importantly, because its much, much
} easier
} to take images at moderate powers and highly enlarge. That way we take
} advantage of the TEM's greater depths of focus at low powers and of
} film's
} higher resolution/ image detail.
} 2 Whenever a TEM image is taken at low brightness (to avoid beam
} damage or at
} very high powers or in dark-field) relatively few electrons form the
} image and
} make that image grainy. Film is very slow and requires then a longer
} exposure,
} thus boosting the quantity of electrons used and improving the image.
} Digital
} is much more sensitive and so the exposure must be shortened. As a
} result the
} best digital camera will record, quiet faithfully the grainy, unsharp
} image.
}
} Many labs rarely or never use such applications and for them the reasons
} to
} change to digital now may be overwhelming.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Wednesday, May 31, 2000 5:18 AM, William Tivol
} [SMTP:tivol-at-wadsworth.org]
} wrote:
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Peter Bond wrote:
} }
} } } The continuing discussion on whether film is better than digital,
} and
} } } whether we can directly compare their resolving ability is very
} interesting
} } } and throwing up some really useful technical stuff. But aren't we
} missing
} } } the point a bit? Surely the image quality is user defined - if you
} or your
} } } customer, are satisfied with the end product then the equipment has
} done
} } } its job. How often does one need to examine a micrograph to assess
} its
} } } limits? If are getting that close to a picture then you may be
} taking
} } } things out of context a bit and losing the whole concept.
} } }
} } } The future is with digital image capture, let's hope the price of
} the
} } } equipment comes down to something more attainable!
} } }
} }
} } Dear Pete,
} } If one is doing quantitative image processing, the better
} } resolution
} } available from film can be relevant. For example, if one wants to do
} } corellation averaging, one needs as many objects in the picture as
} possible,
} } and also as good resolution as needed--e.g., for 1 nm resolution of
} the
} } reconstruction, a pixel size of 0.25 nm times the magnification is
} necessary,
} } so the recording medium must have resolution equal to or better than
} that.
} } The pixel size of the scanner must also be this small--obviously
} irrelevant
} } for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
} } gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one
} will have
} } about 12,000 by 16,000 (taking the header into account) pixels of
} useful
} } information. This will give a broader area than that available at
} equal
} } resolution from any CCD chip now out there. I can assure you that I
} have
} } often had to determine the information limits in a particular
} micrograph.
} } Yours,
} } Bill Tivol
} }
} }
}



From daemon Thu Jun 01 07:11:22 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 1 Jun 2000 09:52:22 +0000
Subject: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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The electron energies used in this "intensifier" will be in the order of one
or several kV. In TEM however the energies are much higher thus one incident
electron will generate for example hundred electron-hole pairs in the CCD
thus saturating it very fast. Another factors are damage to the CCD chip and
X-Rays.

I was thinking about another approach. A chip consisting of matrix of
thermo-sensitive elements. Above each element there will be a metal block
with height equal or larger than the stop path for the electron energy used.
The whole thing will be cooled in a similar way as the CCDs. When this
assembly is exposed to the beam each block will increase its temperature
depending on the number of electrons stopped. After the exposure the matrix
is scanned and the temperature increase at each element is measured
(ofcourse before each exposure a reference image has to be taken).

The benefits:
- Very high efficiency. Almost every incident electron will contribute to
the image.
- Huge dynamic range.
- Linearity (after the temperature-signal characteristic of each element has
been calibrated)
- Narrow point spread function (maybe).

Problems:
- Difficult to manufacture (the metal blocks should be insulated from each
other)
- Maybe low sensitivity. I haven't calculated how much the temperature
increase will be (for example 5x5x30um Cu block hit by one 300 kV electron)
but I suspect it will be very small. Also It will depend on the sensitivity
of the thermo-measuring elements.
- Saturation. After each exposure one has to wait some time for the thing to
cool down again.

There are maybe other difficulties which do not come in mind now.

Hmmm now I start thinking about X-Rays. Actually the main part of the
incident energy will go into X-Rays thus making this device useless.

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Shane Collins {kshanec-at-gte.net}
To: Paul Voyles {voyles-at-research.nj.nec.com} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, June 01, 2000 9:15 AM


AnalySIS has a module which does this
see Soft Imaging's web site at
http://www.soft-imaging.de
Chris

To: Microscopy-at-sparc5.microscopy.com
} From: Jim Ferreira {ferreira1-at-llnl.gov}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Thu Jun 01 07:21:29 2000



From: Colin MacRae :      cmac-at-minerals.csiro.au
Date: Thu, 1 Jun 2000 07:11:59 -0500
Subject: Re: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 5/31/00 2:52:24 PM, ferreira1-at-llnl.gov writes:

Anyone aware of software capable of generating depth profiles from digital
SEM stereo pair images? I am aware of the Oxford ISIS system/program, but
wondered if other companies offer similar software that would run
'stand-alone' in a PC and work with any digital files.

Jim
I have been using uMex software for the last 6 months which does
exactly
what you want. Given a stereo pair it will produce a 3D surface
reconstruction. It has a function that enables images to the two images to
be manually aligned prior to the calculation. This function when used
correctly seems to result in faster calculations.

Another other nice feature is that images can be output in VRML file
format. So you can view the images with shareware 3D packages. We use a
SGI for viewing as its rendering speed is significantly faster than a PC.

I suggest you check out the following web site.
http://www.alicona.com/en/products.htm

Regards
Colin MacRae

************************************************************************
Manager of Electron Microscopy Group (Clayton)

CSIRO
Minerals {mailto:colin.macrae-at-minerals.csiro.au}

PO Box 312, Clayton South, Ph. 61 3 9545 8800
Vic, 3169 Fax 61 3 9562 8919
AUSTRALIA

EM units WWW site http://www.minerals.csiro.au/em-unit/
*************************************************************************




From daemon Thu Jun 01 08:41:17 2000



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Thu, 1 Jun 2000 06:31:32 -0700 (PDT)
Subject: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I've been following the thread on film vs digital.
We all know that the resolving power of digital CCD
faceplates is approaching that of conventional film
emulsion, but isn't equivalent yet.
Can someone remind me of the number of mega-pixels
that a CCD will need to equate to ISO 100 print film,
ISO 64 or ISO100 slide film and the highest resolving
B&W film of all, Technical Pan at ISO 25 and ISO 100?
If anyone out there can lead me through the logic and
the maths, I'm sure others will also find it helpful.
I'll repost a summary of the replies that I get.

Regards, Jeremy Sanderson

__________________________________________________
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Send instant messages & get email alerts with Yahoo! Messenger.
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From daemon Thu Jun 01 16:08:51 2000



From: Paul Voyles :      voyles-at-research.nj.nec.com
Date: Thu, 1 Jun 2000 10:34:26 -0500
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hamamatsu recently introduced a new camera called the electron bombardment
} ccd where accelerated electrons directly bombard a back thinned, peltier
} cooled ccd. The electrons are emitted from a photocathode for applications
} particularly in low light microscopy. With a full well capacity of 300,000
} electrons, I wonder if this approach could be used in direct exposure of the
} ccd to the electron beam.
} Shane

This certainly could be a significant improvement in CCD imaging.
Most of the width of the point spread function of current CCD camera
is due to photon spread in the scintillator, so presumably removing
the scintillator would allow digital images at resolutions very close
to the pixel size of the CCD chip.

I'm not familiar with the Hamamatsu camera, but I know that the
Gatan camera I currently use has a CCD with a full well capacity of
~500,000 electrons. In order for the Hamamatsu chip to work they
would have to find some way to reduce the electron/hole yield of the
incident fast electrons - maybe with a very thin CCD chip?

Paul Voyles


} K. Shane Collins
} Scientific Instrument Company
} 805.444.4953 cell
} 310.568.9188 office
} 310.568.9189 fax
}
}
} -----Original Message-----
} From: Paul Voyles [mailto:voyles-at-research.nj.nec.com]
} Sent: Friday, May 26, 2000 9:11 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: new developments in imaging systems?
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 01 16:08:52 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 01 Jun 2000 11:27:44 -0400
Subject: Re: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeremy Sanderson wrote:

} Dear All,
}
} I've been following the thread on film vs digital.
} We all know that the resolving power of digital CCD
} faceplates is approaching that of conventional film
} emulsion, but isn't equivalent yet.
} Can someone remind me of the number of mega-pixels
} that a CCD will need to equate to ISO 100 print film,
} ISO 64 or ISO100 slide film and the highest resolving
} B&W film of all, Technical Pan at ISO 25 and ISO 100?
} If anyone out there can lead me through the logic and
} the maths, I'm sure others will also find it helpful.
} I'll repost a summary of the replies that I get.
}
} Regards, Jeremy Sanderson
}

About twenty-five million pixels in a 35mm Kodachrome slide is the
number I have heard from several sources, none of which I can remember now.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Jun 01 16:08:52 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 1 Jun 2000 09:23:53 -0600
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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No, it's not really a problem. It's been done with low density
microscopy all the time. Granted, there are some technical aspects to be
overcome, but (and I can only speak for ourselves) we have done that on
a number of microscopes. You are of course correct, that 10 images at
0.8 seconds take longer than 8 seconds as the image has to be
transferred, etc. BUT: that's what beam blankers are for. It is pretty
straightforward to take an image at 0.8 seconds, then blank the beam
very quickly before taking the next image. That way you get pretty close
to the 8 sec total exposure. If there is no beam blanker on the
microscope, in most cases it can be added.

I am not sure what you mean by "too sensitive". The cameras are usually
constructed so that 1 electron from the beam creates between a few tenth
to a few counts (these are all statistical data, of course). The well
width divided by this sensitivity then determines, how many primary
electrons are needed to fully expose one pixel. For example, if the well
width is 50,000 electrons and the sensitivity is 1 count/electron, one
needs 50,000 primary electrons to fill the well. This translates into
roughly a 0.4% statistical error.

} From a practical standpoint: You can take images with most cameras when
the exposure meter on the microscope reads a couple of seconds without
overexposing the camera. On the other hand, you can reduce the intensity
of the beam until you see single electron events.

The one area where CCD cameras may be too sensitive is diffraction. The
normally huge intensity in the transmitted beam often leads to
saturation. In CCDs this can lead to blooming (the intensity spills over
into neighboring pixels). This can be taken care of with special chips
that have anti-blooming features, but this usually has some other
drawbacks. Again, this can also be overcome somewhat with multiple
exposures. Film behaves more civilized here, as it simply stops
responding to the electrons, but this makes film more or less useless
for quantitative measurements of diffraction patterns. I have done
diffraction with CCDs many times and though it does require some
tweaking, one can get very good results from them.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Wednesday, May 31, 2000 9:37 PM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


Yes, it could be done "in theory". Somebody would need to figure out the

software and perhaps modify the hardware. Then we would find that the
total
exposure of the specimen to the electron beam maybe a muliple of the
film's
exposure. Afterall, an 8 sec film exposure would not amount in digital
to
10x0.8, but we would require considerable time in between exposures.
Since the
problems in the discussed circumstances are specimen movement and beam
damage,
it seems that taking multiple exposures is a poor option.

Digital cameras are for some situation too sensitive to electron
exposure.
Cutting back on electrons is no option since its the electrons that form
the
image in the first instance.
Much easier in light microscopy . . . insert a neutral density filter.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com




From daemon Thu Jun 01 16:08:53 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Thu, 1 Jun 2000 11:37:20 -0400
Subject: Re: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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Jim-
I saw an ad a while back for such software. I have since moved on
to other things before I had a chance to obtain the demo software. Below is the
contact information I have for the company selling the stereo image analysis software.
Since this is an edited version of the message I received several months
ago, the terms and conditions may have changed.
If you do try this software, I, and I am sure the rest of the listserve would be very
interested in hearing how well it
works, as I do have an occational need for this capability.

Here is the information (edited) that I received from the company offering the software:
now the evaluation version of MeX is available.
The test period of MeX is limited to 6 weeks.

The evaluation version is delivered with a small database and the
complete analysis tools. You can process your own images without
restrictions.

We also offer to preinclude your SEM-images into the database of the
evaluation version. You just have to send us an email and we give you
detailed information on how you should capture your images.


If you have any questions, please do not hesitate to contact us.


Kind regards, Johann Schweiger

=====================================
= Dipl.-Ing.(FH) Johann Schweiger
= Sales
= Alicona GdbR
= Koch-Sternfeldstr. 5
= D-83471 Berchtesgaden/Germany
= Tel. ++49 8652 964205
= Fax. ++49 8652 964207

I hope that you find the above information helpful.
Sincerely,
Matthew H. Ervin, Ph.D.



From daemon Thu Jun 01 16:08:53 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 1 Jun 2000 09:39:59 -0600
Subject: RE: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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Jim,
you may want to check out our web site for the Stereo software. It has
some images and examples of stereo evaluations from SEM images. The
'Stereo' part is not a stand-alone software, but can be combines with
our analySIS Docu software for a stand-alone application.
Michael


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

Hello folks
Anyone aware of software capable of generating depth profiles from
digital SEM stereo pair images? I am aware of the Oxford ISIS
system/program, but wondered if other companies offer similar software
that would run 'stand-alone' in a PC and work with any digital files.
Any help or suggestions along these lines would be most appreciated.
Thanks,
Jim







From daemon Thu Jun 01 16:08:53 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Thu, 1 Jun 2000 15:24:59 +0100
Subject: Wet Specimens

Contents Retrieved from Microscopy Listserver Archives
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Hi

Want to work with wet specimens?

Do not give up if you do not have an ESEM or LVSEM there may be hope yet?
Do you have a SEM with a rear manifold that connects directly to the DP and
do you have a BSE detector? Sorry but those SEM that have the pump directly
attached to the specimen chamber are no good for this procedure. AND I must
give all the credit to Viv Robinson who spawned this idea back in the 80's.

If you do have a manifold system you are in luck. Find a rubber bung that
will fit into the manifold at the rear of the specimen chamber. Drill (use
LN2) a 1/4" hole in the bung and then place it in the rear manifold. Switch
off or better still unplug your Everhart-Thornley detector (high voltage
plus poor vacuum = arcing!). Place you "wet" specimen in the microscope and
pump down. The bung will spoil the vacuum in the chamber for about 20
minutes or so and imaging with the BSE detector you will have your own LV
SEM.

To retain the moisture longer you may quench the specimen in LN2 before
putting it into the microscope. The frost will sublime away and you will be
able to watch what happens to the moisture etc.

We use this technique on all sorts of samples. A rubber bung is cheaper than
buying an LVSEM and the results, if you work quickly, are pretty good.

Try it?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Thu Jun 01 16:08:54 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 1 Jun 2000 17:10:23 +0000
Subject: Re: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
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Is it just my problem, or isn't this all getting a little bit unfocussed!
As far as TEM is concerned, the CCD is not directly exposed to
the beam at all, so its effective resolution depends on the nature of
the system that presents the image to the CCD. The two
predominating technologies for transfer of the image to the CCD in
TEM cameras are a fibre-optic linkagage between a phosphor or
YAG scintillator and the CCD, or an optical coupling via a lens (for
example the excellent f1.2 50mm Zuiko macro lens by Olympus).
In both instances, the ultimate resolution of the system is probably
set by the electron sensor, which is the phosphor or YAG
scintillator. I suspect that fibre optic couplings probably degrade
that resolution, but I say that without reference to the facts, so
please correct me if I am mistaken. Optical coupling could in
principle project the spatial data recorded by the phosphor or YAG
to any desired magnification. It can thus be recorded by a CCD
using many pixels or few depending on the optical configuration.
So what do we mean by resolution in this context, when the
smallest object which can be imaged by a TEM can be projected
onto any desired quantity of CCD pixels?

To begin to answer Jeremy's question directly, we need to know
how much detail a Technical Pan negative can record. The figures
depend on processing technique and the test object luminance and
contrast, but the modulation transfer function figures published by
Kodak indicate that a spatial frequency in excess of 200 cycles per
mm is easily recordable. For a test object with contrast 100:1 they
quote 320 line pairs per mm. The CCD pixel spacing required to
achieve this feat would be 640 pixels per mm. That equates to a
requirement for 15360 x 23040 pixels to match the resolving power
of a 24x36mm Technical Pan exposure. That's 0.35 Giga pixels in
round numbers.

So what was that I heard about the death of silver imaging? I don't
think so. Not for a while yet.

Best wishes
Chris

} Dear All,
}
} I've been following the thread on film vs digital.
} We all know that the resolving power of digital CCD
} faceplates is approaching that of conventional film
} emulsion, but isn't equivalent yet.
} Can someone remind me of the number of mega-pixels
} that a CCD will need to equate to ISO 100 print film,
} ISO 64 or ISO100 slide film and the highest resolving
} B&W film of all, Technical Pan at ISO 25 and ISO 100?
} If anyone out there can lead me through the logic and
} the maths, I'm sure others will also find it helpful.
} I'll repost a summary of the replies that I get.
}
} Regards, Jeremy Sanderson
}
} __________________________________________________
} Do You Yahoo!?
} Send instant messages & get email alerts with Yahoo! Messenger.
} http://im.yahoo.com/
}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Thu Jun 01 16:08:54 2000



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Thu, 01 Jun 2000 09:31:04 -0700
Subject: Re: curing Epon/Araldite with UV?

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We at Ted Pella Inc. have used our Pelco UVC2 UV Cryo Chamber to cure epoxies
but most of our UV curing has been with Acrylic resins. The literature we have
on Eponate Araldite does not show it to be UV curable but our chemist wouldn't
be surprised if it didn't accelerate the cure. The epoxy mixture should cure
overnight at 60 degrees C. We have a technical note on the use of
Epon-Araldite for embedding specimens and literature on our Cryo Chamber that
may help. I would be happy to fax them to you and/or have you talk with our
chemist.

Mark J Armogida
VP Engineering and Production
Ted Pella Inc.

Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Greetings!
}
} Has anyone ever cured Epon-Araldite with UV? I was given a
} cobbled-together protocol by a non-microscopist post-doc; the protocol
} calls for a dry-ice, UV cure of Epon-Araldite. It says it can take from
} 3-7 days. I'm wondering if this is either a resin mix-up *or* the resin
} just set up all by itself (over that time frame I've seen it happen), just
} because it was a thin layer and left alone - nothing to do with the UV
} exposure. She has no ref. for this technique and isn't sure now where she
} got it. Sigh. I've only been able to find heat-cure protocols...anyone
} have any leads or thoughts on this UV thing?
}
} (Sorry about the cross-post for those of you on both of these servers)
}
} Thanks!
}
} Tamara (Planning to use the oven.....) Howard
} CSHL



From daemon Thu Jun 01 16:08:55 2000



From: Linda Boisselle :      THE-LAB-at-worldnet.att.net
Date: Thu, 01 Jun 2000 14:02:00 -0400
Subject: Seeking help

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I am seeking to speak to individuals who know about designing and
developing applications for indentification of rare cells in microscopic
biological preparations. Someone who knows how to optimize microscopy
autofocusing procedures. I am more interested in speaking to an
electrical engineer who is more into digital image processing. Can
anyone suggest what direction to take?

THE-LAB-at-att.net

Thanks.



From daemon Thu Jun 01 16:09:00 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 1 Jun 2000 15:14:38 -0500
Subject: TEM: looking for phage

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We are trying to help a client find and identify a bacteriophage. His
bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
areas with sharp edges where the phage are supposed to be. So far, we have
taken carbon-coated grids and placed them gently onto the surface of the
clear areas, then lifted them off and negative-stained with PTA or uranyl
acetate. We have also run buffer across the clear areas, then pipetted it
onto the grids and stained it. A microbiologist who works with phage in
another lab has taken samples from the clear areas and concentrated them
down and we have stained those also.

So far we have found exactly two phage-like organisms in a total of about 10
grids. Not a stellar performance.

We figure the possibilities are: 1) the bacteria are being killed by
something other than phage; 2) we're looking for a particular type of phage
that may not be there, and we're just not seeing what's actually causing the
clear areas, or 3) for some reason we're just not getting the things
adhering to the grids, although we've used these methods successfully many
times before.

Does anyone else have any ideas that might help us out, especially on
technique? Our client is almost certain that phage are present. We just
can't find them.

Thanks in advance.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Jun 01 17:58:48 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 1 Jun 2000 12:46:37 -1000 (HST)
Subject: SEM: immunolabeling

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One of our SEM users would like to label biofilms with lectins. Three of
them. At once. I have used gold conjugated to goat anti-biotin to label
biotinylated lectins for TEM in the past, so I'm hoping to follow the same
kind of procedure. I have not done any immunolabeling for SEM, although
our FESEM has been used for such. I would be grateful for any advice! If
he wants to triple label, what sizes of gold would be useful? If he wants
to quantify the three, what kinds of controls for labeling efficiency
should we run? All hints ahd tips gratefully accepted!

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Jun 01 17:58:49 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 1 Jun 2000 12:50:40 -1000 (HST)
Subject: Confocal: gold enhancement

Contents Retrieved from Microscopy Listserver Archives
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I have not generally followed any discussion on gold enhancement for light
microscopy (photons? I don't do photons), but now I need to ask for
someone what people suggest for immunogold enhancement for confocal
microscopy. We have 10nm gold left over from TEM, and it would be useful
to use it for the confocal experiment. Yes, we're being cheap!

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Jun 01 20:59:23 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 1 Jun 2000 20:40:40 -0500
Subject: Re: Resolving power: film vs digital

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} From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com}
{snip}
} To begin to answer Jeremy's question directly, we need to know
} how much detail a Technical Pan negative can record. The figures
} depend on processing technique and the test object luminance and
} contrast, but the modulation transfer function figures published by
} Kodak indicate that a spatial frequency in excess of 200 cycles per
} mm is easily recordable. For a test object with contrast 100:1 they
} quote 320 line pairs per mm. The CCD pixel spacing required to
} achieve this feat would be 640 pixels per mm. That equates to a
} requirement for 15360 x 23040 pixels to match the resolving power
} of a 24x36mm Technical Pan exposure. That's 0.35 Giga pixels in
} round numbers.
}
At 16 bits this is per image this is 5,662,310,400 or 5.5 gig. I don't
think film is in any danger for a long time. We need at least 1 order
of magnitude for storage and 2 or 3 for processing. I remember
taking 4 days to process an image. And then work on the program and
trigger it again.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00








From daemon Fri Jun 02 08:39:48 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Fri, 02 Jun 2000 11:14:47 +0200
Subject: Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
This is the last call for a second post-doc vacancy at the
University of Barcelona (Spain) to work in the frame of a TMR
programme concerning UV coatings. (More details below).

Since we are expecting the Mid-Term evaluation of the project,
the starting date has been delayed to next September, so we
have extended the deadline for applications.

Any one interested please reply directly to paqui-at-el.ub.es
and/or send applications and a CV by mail before 30 June 2000.

Kind regards

F. Peir—

**************************************************************************
Laboratory: Electronic Materials and Engineering, Department of
Electronics, University of Barcelona.

Duration: 12-months, starting September 2000.



Hi Jim, some comments to Your remarks within Your text:

jim schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} Yes, it could be done "in theory". Somebody would need to figure out the
} software and perhaps modify the hardware. Then we would find that the total
} exposure of the specimen to the electron beam maybe a muliple of the film's
} exposure. Afterall, an 8 sec film exposure would not amount in digital to
} 10x0.8, but we would require considerable time in between exposures.

Simply choose a CCD with higher readout performance (faster), but same quality.

} Since the
} problems in the discussed circumstances are specimen movement and beam damage,
} it seems that taking multiple exposures is a poor option.
}
} Digital cameras are for some situation too sensitive to electron exposure.

Not correct at all. The only thing is to choose the correct camera for the
application You work on. It is not complicated to make a digital system which
collects one count per incident electron to achieve the same signal to noise as in
the electron beam. This system will be less sensitive than normally sold systems
but the main advantage of digital systems that You see what You get remains and You
get instant results of Your work.
The only problem You have to solve for these systems is to use a not very sensitive
scintillator but a very high performance slow-scan CCD. So You get less visible
photons from Your electron which have to be converted in one digital count. But
these digital counts must be more than presently available to achieve a good
statistic. Thats the reason for our new 16bit CCD (dynamic up to 65536 digits) for
high performance TEM applications.

}
} Cutting back on electrons is no option since its the electrons that form the
} image in the first instance.
} Much easier in light microscopy . . . insert a neutral density filter.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Fri Jun 02 08:39:50 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 2 Jun 2000 11:18:10 +0000
Subject: Re: Resolving power: film vs digital

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} About twenty-five million pixels in a 35mm Kodachrome slide is the
} number I have heard from several sources, none of which I can remember now.
}
} Geoff
Geoff
Analysing this a little further:
A 25 Mbyte image = 8.3 M pixels to cover 24x36mm or 864mm^2
this represents 98.2 pixels per mm, or 49 line pairs per mm. Pixel
size 10.2 um. Resolution about 50 line pairs per mm at best.

A 25M pixels image used to capture a 24x36mm Kodachrome
slide represents 28,935 pixels per mm^2 or 170 pixels per mm.
This is equivalent to a maximum resolution of 85 line pairs per mm,
which may be on the conservative side for Kodachrome. pixel size
= 5.88um. The image will be approx. 6120x4082 pixels, generating
a file size of approx 75Mb for a 24-bit (8+8+8bit) RGB image.

To record 120 line pairs per mm, which many top 35mm camera
lenses can achieve, a minimum of 240 pixels per mm are required,
each 4.2 um wide. This equates to 8640x5760 pixels for a
24x36mm frame = 50M-pixels or 50Mb in 8-bit greyscale, or 150Mb
in 24-bit RGB.

At 320 line pairs per mm (Technical Pan) the minimum required
640 pixels per mm is a pixel size of 1.56 um

Presumably for a light image the diffraction limited resolution is
approx 1/2 lambda which at 540nm is 0.27um.
So looking to the future of ultimate-performance CCDs, direct
recording of a diffraction limited light image projected onto the
sensor requires at the very least 3703 pixels per image mm or
13,717,421 pixels per mm^2 (greyscale 8-bit)

However, if we are doing light microscopy with an NA 1.4 x100
objective, how much resolving power do we need on CCD or film?

Data is at 0.27um resolution (lambda = 540nm). Let's round this to
0.3um. Magnification at 24x36mm film image is x100, so pixels
must be an absolute maximum of 30um wide to record the
significant data = 33.3 pixels per mm, equivalent to 800x1200
pixels to record the whole 35mm negative area. However, most
CCDs are much smaller than 35 mm frames, typically 1/3 inch. So
an 800x1200 pixel CCD at 8x12 mm, 1/3 of the linear dimensions
of a 35mm frame would use 9 pixels to record the smallest image
details. This is about right from the point of view of resolution, but
to record the whole 35mm frame we need about 2400x3600 pixels
on our CCD.

Note also that Technical Pan has (depending on the criterion used
to assess its performance) up to 10 times the resolving power
required to record all there is to see in a diffraction-limited LM
image made with a 100x NA 1.4 lens. So you can comfortably
afford to use a 60x NA 1.4 lens, thereby getting the same
resolution with a bigger field of view.

Many years ago, I took a photograph of a street scene using a
Canon 35mm SLR loaded with Kodak Recordak (I think this was a
single layer microfilm emulsion). Examined in a light microscope,
the image clearly, legibly recorded the brand-name of a child's
push chair. I tried to print this brand name using a DeVere point-
source enlarger with an image size of 20x30 inches produced with
a Schneider Componon lens, but was completely unable to
produce a legible image. The point I am making here is that the
combination of some high performance films, with high quality
lenses of the standard produced by the leading camera
manufacturers can record more detail on the film than you can
easily get back out by conventional printing. I suspect the same is
true of EM exposures.

So there is no contest - film beats CCDs for resolution hands
down. And you can process the image on the cheap. No money
goes to Intel or Microscoft, Adobe or Epson. But resolution is not
primarily what we buy CCDs for. We buy them primarily for instant
image capture in a format suitable for digital storage, digital
transmission quantitative data recording and image processing.

Chris

=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Fri Jun 02 08:39:55 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 2 Jun 2000 21:15:36 +1000
Subject: RE: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
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Gordon's and Chris' contribution look very bleak for digital, but the
comparison is not really fair, as we should look at the practical aspects too.
The unaided eye resolves lines 0.1mm apart, so to see the full (200 black+200
white) 400+ lines/mm that may be recorded on TEM film we would need to enlarge
over 40x. This requires an enlarger with a very wide angle lens to print small
portions of the negative and it is technically difficult to so enlarge a whole
negative since a 4" negative becomes 160" or over 4m in size. That degree of
enlargement is not fully useful since in a well exposed TEM negative at just
under 30x electron noise becomes the problem, meaning not enough electrons
contributed to the image. So enlargements beyond 30x are empty- and provide no
further information. Truly not a serious problem.
The practical part is that few people ever find it useful to enlarge more than
15x and most TEM images reproduced are barely the size of the original
negative, however, they are enlarged from a smaller portion thereof.
For these most common applications, digitals with 10+ megabytes provide
excellent image details and tonal gradation. However, that size image can only
cover the equivalent of a small 35mm negative equivalent and does not allow
high enlargement or choosing of an adjacent field.
It appears that the best of both worlds is the use of conventional TEM
negatives for archiving and scanning those as required for printing.

It should be noted that this discussion concerns TEM. Digitals in SEM are less
daunting and it is not problematical to produce excellent digitals, comparable
with film.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, June 02, 2000 11:41 AM, Gordon Couger [SMTP:gcouger-at-couger.com]
wrote:
}
}
} } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com}
} {snip}
} } To begin to answer Jeremy's question directly, we need to know
} } how much detail a Technical Pan negative can record. The figures
} } depend on processing technique and the test object luminance and
} } contrast, but the modulation transfer function figures published by
} } Kodak indicate that a spatial frequency in excess of 200 cycles per
} } mm is easily recordable. For a test object with contrast 100:1 they
} } quote 320 line pairs per mm. The CCD pixel spacing required to
} } achieve this feat would be 640 pixels per mm. That equates to a
} } requirement for 15360 x 23040 pixels to match the resolving power
} } of a 24x36mm Technical Pan exposure. That's 0.35 Giga pixels in
} } round numbers.
} }
} At 16 bits this is per image this is 5,662,310,400 or 5.5 gig. I don't
} think film is in any danger for a long time. We need at least 1 order
} of magnitude for storage and 2 or 3 for processing. I remember
} taking 4 days to process an image. And then work on the program and
} trigger it again.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00



From daemon Fri Jun 02 08:39:56 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 2 Jun 2000 21:14:44 +1000
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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The world is full of possible solutions, but are they practical.?
To produce high-resolution, dark-field or any others TEM images that require
more electrons to form a clear image, Mike Bode would use multiple digital
exposures. The exposures could be layered and combined into one superior image.
This image would be made up of more pixel and is formed by more electrons and
so would be noise-free and hence could be further enlarged then otherwise
possible. Perhaps.
Beam blanking would largely save the specimen from beam damage and drift could
be compensated for by matching up the digitals. Great.
How much time is required between exposures to transfer a minimum 10mb image
per exposure? What would be the total time from focusing to the last exposure?
What about Z-drift in the interim requiring objective changes and what about
the total cost of this additional get-up. The mind boggles at a through focus
series.
When pushing the limits a piece of film seems more effective, cheaper and fa
ster.
Again, I don't doubt that there is now a large place for digital in TEM, but
its no panacea.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com] wrote:
}
} No, it's not really a problem. It's been done with low density
} microscopy all the time. Granted, there are some technical aspects to be
} overcome, but (and I can only speak for ourselves) we have done that on
} a number of microscopes. You are of course correct, that 10 images at
} 0.8 seconds take longer than 8 seconds as the image has to be
} transferred, etc. BUT: that's what beam blankers are for. It is pretty
} straightforward to take an image at 0.8 seconds, then blank the beam
} very quickly before taking the next image. That way you get pretty close
} to the 8 sec total exposure. If there is no beam blanker on the
} microscope, in most cases it can be added.
}
} I am not sure what you mean by "too sensitive". The cameras are usually
} constructed so that 1 electron from the beam creates between a few tenth
} to a few counts (these are all statistical data, of course). The well
} width divided by this sensitivity then determines, how many primary
} electrons are needed to fully expose one pixel. For example, if the well
} width is 50,000 electrons and the sensitivity is 1 count/electron, one
} needs 50,000 primary electrons to fill the well. This translates into
} roughly a 0.4% statistical error.
}
} } From a practical standpoint: You can take images with most cameras when
} the exposure meter on the microscope reads a couple of seconds without
} overexposing the camera. On the other hand, you can reduce the intensity
} of the beam until you see single electron events.
}
} The one area where CCD cameras may be too sensitive is diffraction. The
} normally huge intensity in the transmitted beam often leads to
} saturation. In CCDs this can lead to blooming (the intensity spills over
} into neighboring pixels). This can be taken care of with special chips
} that have anti-blooming features, but this usually has some other
} drawbacks. Again, this can also be overcome somewhat with multiple
} exposures. Film behaves more civilized here, as it simply stops
} responding to the electrons, but this makes film more or less useless
} for quantitative measurements of diffraction patterns. I have done
} diffraction with CCDs many times and though it does require some
} tweaking, one can get very good results from them.
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Wednesday, May 31, 2000 9:37 PM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Film vs Digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Yes, it could be done "in theory". Somebody would need to figure out the
}
} software and perhaps modify the hardware. Then we would find that the
} total
} exposure of the specimen to the electron beam maybe a muliple of the
} film's
} exposure. Afterall, an 8 sec film exposure would not amount in digital
} to
} 10x0.8, but we would require considerable time in between exposures.
} Since the
} problems in the discussed circumstances are specimen movement and beam
} damage,
} it seems that taking multiple exposures is a poor option.
}
} Digital cameras are for some situation too sensitive to electron
} exposure.
} Cutting back on electrons is no option since its the electrons that form
} the
} image in the first instance.
} Much easier in light microscopy . . . insert a neutral density filter.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}



From daemon Fri Jun 02 09:02:14 2000



From: Horst Neve :      neve-at-bafm.de
Date: Fri, 2 Jun 2000 08:40:59 -0500
Subject: TEM - Looking for phage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

if possible, select plates obtained from serial dilutions which show
confluent lysis (i.e., where plaques touch each other). They are a good
source for obtaining high-titer bacteriophage lysates (i.g., 10E10 / 10E11
plaque-forming units per ml). Harvest the phage by scraping the top agar
and transfer it into a test tube or equivalent. Rinse the plates with some
ml of phage diluent (i.g., 5 ml). Shake this supension containing phage,
host cells and agar for some while before spinning down the cells and the
agar. It is also a good idea to pick up a single plaque. Resuspend it in a
small volume of broth, and prepare a fresh lysate in some ml of broth with
fresh host cells. For rapid screening, we sometimes pipette a drop of
} buffer onto a plaque and float a piece of carbon film into the drop
directly from a mica support. After some minutes, we pick up the film with
a grid and do the routine negative staining. But you are right, the number
of phage particles is low in this case.
Best regards
Horst Neve

} At 15:14 01.06.00 -0500, you wrote:
} Hi,
}
} We are trying to help a client find and identify a bacteriophage. His
} bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
} areas with sharp edges where the phage are supposed to be. So far, we have
} taken carbon-coated grids and placed them gently onto the surface of the
} clear areas, then lifted them off and negative-stained with PTA or uranyl
} acetate. We have also run buffer across the clear areas, then pipetted it
} onto the grids and stained it. A microbiologist who works with phage in
} another lab has taken samples from the clear areas and concentrated them
} down and we have stained those also.
}
} So far we have found exactly two phage-like organisms in a total of about 10
} grids. Not a stellar performance.
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211

****************************************************************************
****
Dr. Horst Neve
Institut fuer Mikrobiologie / Institute for Microbiology
Bundesanstalt fuer Milchforschung / Federal Dairy Research Centre
Postfach / P.O. Box 6069, D-24121 Kiel
Hermann-Weigmann-Str. 1, D-24103 Kiel
****************************************************************************
****
Tel. / Phone: +49 (0) 431 609 2343 {} Fax: +49 (0) 431 609 2306
E-mail: neve-at-bafm.de {} Internet: http://www.bafm.de
****************************************************************************
****




From daemon Fri Jun 02 09:02:14 2000



From: Horst Neve :      neve-at-bafm.de
Date: Fri, 2 Jun 2000 08:42:08 -0500
Subject: TEM - Looking for phage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

if possible, select plates obtained from serial dilutions which show
confluent lysis (i.e., where plaques touch each other). They are a good
source for obtaining high-titer bacteriophage lysates (i.g., 10E10 / 10E11
plaque-forming units per ml). Harvest the phage by scraping the top agar
and transfer it into a test tube or equivalent. Rinse the plates with some
ml of phage diluent (i.g., 5 ml). Shake this supension containing phage,
host cells and agar for some while before spinning down the cells and the
agar. It is also a good idea to pick up a single plaque. Resuspend it in a
small volume of broth, and prepare a fresh lysate in some ml of broth with
fresh host cells. For rapid screening, we sometimes pipette a drop of
} buffer onto a plaque and float a piece of carbon film into the drop
directly from a mica support. After some minutes, we pick up the film with
a grid and do the routine negative staining. But you are right, the number
of phage particles is low in this case.
Best regards
Horst Neve

} At 15:14 01.06.00 -0500, you wrote:
} Hi,
}
} We are trying to help a client find and identify a bacteriophage. His
} bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
} areas with sharp edges where the phage are supposed to be. So far, we have
} taken carbon-coated grids and placed them gently onto the surface of the
} clear areas, then lifted them off and negative-stained with PTA or uranyl
} acetate. We have also run buffer across the clear areas, then pipetted it
} onto the grids and stained it. A microbiologist who works with phage in
} another lab has taken samples from the clear areas and concentrated them
} down and we have stained those also.
}
} So far we have found exactly two phage-like organisms in a total of about 10
} grids. Not a stellar performance.
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211

****************************************************************************
****
Dr. Horst Neve
Institut fuer Mikrobiologie / Institute for Microbiology
Bundesanstalt fuer Milchforschung / Federal Dairy Research Centre
Postfach / P.O. Box 6069, D-24121 Kiel
Hermann-Weigmann-Str. 1, D-24103 Kiel
****************************************************************************
****
Tel. / Phone: +49 (0) 431 609 2343 {} Fax: +49 (0) 431 609 2306
E-mail: neve-at-bafm.de {} Internet: http://www.bafm.de
****************************************************************************
****




From daemon Fri Jun 02 10:04:04 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Fri, 02 Jun 2000 08:23:17 -0600
Subject: Incubators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microbiologist experts:

I'm looking for a (preferably) bench top incubator. Non-water jacketed, not using B.O.D. bottles, unit needs to be using CFC free refrigeration system. Does anyone know of such a unit or where one can be purchased? Need to order one ASAP. We are a small research lab and the one we have is a monster (48"Hx46"Wx28"deep and weighs almost 500lbs.). I'd appreciate any info.

Thanks,

Phil Rutledge
prutledge-at-ars.usda.gov



From daemon Fri Jun 02 10:04:04 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Fri, 2 Jun 2000 10:50:05 -0400
Subject: M&M2000: Social Event Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy & Microanalysis 2000 Local Arrangements Committee
is pleased to announce an additional social event at M&M2000---

TAKE ME OUT TO THE BALL GAME !!

Join us on Tuesday, August 15th as the
PHILADELPHIA PHILLIES meet the
ARIZONA DIAMONDBACKS at Veterans Stadium.
Game time is 7:35 PM.

For $32.50, you'll get:
- a ticket to the game (300 level, on the third base line)
- a $10.00 coupon, good toward purchases at all concession and souvenir stands
- round-trip transportation from the Convention Center to the stadium
on a luxury coach (buses leave at 6:00 PM; return to the convention
center immediately following the game)

A limited number of packages are still available. First come, first served.

Checks, in US$ only, payable to "Microscopy & Microanalysis 2000" can be mailed
to:

Bev Maleeff
Treasurer, M&M2000 LAC
c/o SmithKline Beecham Pharmaceuticals
Mail Code UE 0462
709 Swedeland Road
King of Prussia, PA 19406

Checks only, please. No credit cards accepted.
Your cancelled check will serve as your receipt.

Tickets will be distributed at the M&M2000 Hospitality Booth
on Monday and Tuesday, August 14th & 15th.

Don't miss out on the fun!!!

Further information about this and other M&M2000 events can be found on our
website:
http://www.msa.microscopy.com/~mm2000/

See you in August!!

Bev Maleeff
M&M2000 LAC




From daemon Fri Jun 02 10:24:01 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 2 Jun 2000 09:16:34 -0600
Subject: TEM: Freeze Fracture help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Date sent: Fri, 02 Jun 2000 11:03:03 -0400
To: c.jeffree-at-ed.ac.uk
} From: joe fu {jofu-at-nist.gov}


I have a researcher here at USU who would like to have some freeze
fracture preps made of lysosome. Is there anyone out there who is
currently doing FF preps and would be willing to assist us? I can image
the preps here in Logan.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920



From daemon Fri Jun 02 10:43:58 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 2 Jun 2000 10:43:42 -0500
Subject: Re: TEM: looking for phage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rando,

Having worked with a variety of phages (Candida, Streptococcus, E.
coli) I can tell you that finding phages from an agar plaque is very
difficult--as you have determined. The best way is to do a liquid
culture and then high speed followed by ultracentrifugation to
concentrate the particles. FYI, as I recall, on a 200 mesh grid each
virus particle is roughly equivalent to 3.4 x 10E6 vp/ml. So, you
need a lot of particles to even find one of them using this approach.

JB
####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Jun 02 11:53:48 2000



From: Ruth Yamawaki :      yamawaki-at-stanford.edu
Date: Fri, 2 Jun 2000 09:48:03 -0700
Subject: Outside electrical near the EM lab

Contents Retrieved from Microscopy Listserver Archives
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My EM lab is going to be moved and I have just seen the plans. Apparently
two large equipment room are going to be built, one right across the hall
and the other two doors down. I have been told that the equipment room will
house -80 freezers, centrifuges, etc. Does anyone out there have experience
with this type of equipment near their TEM? I will not be able to test the
room before the move because nothing has been built yet and we all scheduled
to move in at the same time. I am hoping to have some influence on the
architects now. They have been told (and I shall keep reminding them) that
no electrical circuits are to be passed around the EM room.

Thanks.

Ruth

**********************************************
Ruth Yamawaki
Department of Comparative Medicine
Building 330, Quad 7, RAF-1
Stanford CA 94305
(650) 723-3457
**********************************************



From daemon Fri Jun 02 12:13:44 2000



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Fri, 2 Jun 2000 14:36:59 -0500
Subject: RE: Outside electrical near the EM lab

Contents Retrieved from Microscopy Listserver Archives
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Well, let's see:

Jim wrote:

Mike Bode would use multiple digital
exposures. The exposures could be layered and combined into one superior
image.
This image would be made up of more pixel and is formed by more
electrons and
so would be noise-free and hence could be further enlarged then
otherwise
possible. Perhaps.

No, I did not talk about further enlargements. All I wanted to say is,
that a more noise-free image can be achieved by adding multiple images,
and that this also to some extent helps with drift of the sample during
acquisition.

Jim wrote:

How much time is required between exposures to transfer a minimum 10mb
image
per exposure?

How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
information is about 2.5 MB (uncompressed). We acquire about 10 of those
per second and transfer them across the PC bus to the display. Putting
them on them into Memory might add a few tenth of a second. Writing to
HD can be done after all images are acquired.

Jim wrote:

What would be the total time from focusing to the last exposure?
What about Z-drift in the interim requiring objective changes

Why would we have to worry about that, if we don't have to worry about
that when taking the image on film? In fact, we could take care of this
by looking at the image between exposures and correct for z-drift.
However, as you said, that would add to the overall time and exposure. I
was comparing a normal dark field image taken on film at 8 seconds with
acquiring the same image on a "too sensitive" CCD camera by adding up 10
consecutive .8 second images. Why would the sample drift (in x, y or z)
substantially more in 8+delta seconds than in 8?

Jim wrote:

what about the total cost of this additional get-up

That of course depends on the microscope and there is no general answer.
For example on a LEO 912 I believe the blanker is standard. The
additional cost to use an acquisition scheme like this with our software
is $0 plus perhaps a bit of time to write a small macro. On other
microscopes one might have to add a beam blanker and perhaps a control
mechanism for the beam blanker. But I would guess, that this cost is not
very high. All modern microscopes are computer controller anyway, so it
is most likely just a control command that needs to be sent to the
microscope over a serial port if the beam blanker is installed. Piece of
cake.

Jim wrote:

The mind boggles at a through focus series.

You're right here. But I don't think we were talking about through-focus
series. Incidentally, we do through-focus series on light microscopes
and reconstruction routinely. Takes a few images at different focus (or
for a light microscope: stage) settings. The rest is done off-line.
Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
agree that TEM is different here and much more complicated due to the
complicated Contrast Transfer Function. However, this could in principle
be sorted out.

Jim wrote:

Again, I don't doubt that there is now a large place for digital in TEM,
but
its no panacea.

I also agree with you on that one. But using the additional computer
possibilities of digital imaging might take you further than expected.

Michael


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Friday, June 02, 2000 5:15 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
Cc: 'jim-at-proscitech.com.au'


This can be hard to predict. Once we were having trouble with rather huge
(100 eV) energy fluctuations in our GIF 200 energy filter. We traced the
source to an adjoining room filled with constant-temperature ovens, fans,
and other high-current equipment. The unlikely source finally turned out to
be a $50 hot plate-stirrer!

Keeping the AC circuitry from running near the lab is a very good
start- I know this has devastated other labs. Ask for your own independent
electrical ground for the 'scope, and that all electrical circuits to your
lab remain independent of other rooms. I doubt that the equipment you
describe will be a big problem as long as you don't share AC circuits,
ground, or a common wall.
Good luck.


"The chief source of problems is solutions."
-Eric Sevareid

...........................................................................
......................................................
Jeffrey A. Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439-4837

(630) 252-5594 (voice)
(630) 252-4771 (fax)





} ----------
} From: Ruth Yamawaki
} Sent: Friday, June 2, 2000 11:48 AM
} To: 'Microscopy-at-sparc5.microscopy.com'
} Subject: Outside electrical near the EM lab
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My EM lab is going to be moved and I have just seen the plans. Apparently
} two large equipment room are going to be built, one right across the hall
} and the other two doors down. I have been told that the equipment room
} will
} house -80 freezers, centrifuges, etc. Does anyone out there have
} experience
} with this type of equipment near their TEM? I will not be able to test
} the
} room before the move because nothing has been built yet and we all
} scheduled
} to move in at the same time. I am hoping to have some influence on the
} architects now. They have been told (and I shall keep reminding them)
} that
} no electrical circuits are to be passed around the EM room.
}
} Thanks.
}
} Ruth
}
} **********************************************
} Ruth Yamawaki
} Department of Comparative Medicine
} Building 330, Quad 7, RAF-1
} Stanford CA 94305
} (650) 723-3457
} **********************************************
}
}



From daemon Fri Jun 02 16:23:08 2000



From: Suzannah Mayo :      smayo-at-southernmicro.com
Date: Fri, 02 Jun 2000 17:02:06 -0400
Subject: Re: TEM - Looking for phage

Contents Retrieved from Microscopy Listserver Archives
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There is a standard technique for phage isolation and purification in Maniatis.
I have found it to work better than any kit and can be modified accordingly.

Horst Neve wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Randy,
}
} if possible, select plates obtained from serial dilutions which show
} confluent lysis (i.e., where plaques touch each other). They are a good
} source for obtaining high-titer bacteriophage lysates (i.g., 10E10 / 10E11
} plaque-forming units per ml). Harvest the phage by scraping the top agar
} and transfer it into a test tube or equivalent. Rinse the plates with some
} ml of phage diluent (i.g., 5 ml). Shake this supension containing phage,
} host cells and agar for some while before spinning down the cells and the
} agar. It is also a good idea to pick up a single plaque. Resuspend it in a
} small volume of broth, and prepare a fresh lysate in some ml of broth with
} fresh host cells. For rapid screening, we sometimes pipette a drop of
} } buffer onto a plaque and float a piece of carbon film into the drop
} directly from a mica support. After some minutes, we pick up the film with
} a grid and do the routine negative staining. But you are right, the number
} of phage particles is low in this case.
} Best regards
} Horst Neve
}
} } At 15:14 01.06.00 -0500, you wrote:
} } Hi,
} }
} } We are trying to help a client find and identify a bacteriophage. His
} } bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
} } areas with sharp edges where the phage are supposed to be. So far, we have
} } taken carbon-coated grids and placed them gently onto the surface of the
} } clear areas, then lifted them off and negative-stained with PTA or uranyl
} } acetate. We have also run buffer across the clear areas, then pipetted it
} } onto the grids and stained it. A microbiologist who works with phage in
} } another lab has taken samples from the clear areas and concentrated them
} } down and we have stained those also.
} }
} } So far we have found exactly two phage-like organisms in a total of about 10
} } grids. Not a stellar performance.
} }
} } Thanks in advance.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
}
} ****************************************************************************
} ****
} Dr. Horst Neve
} Institut fuer Mikrobiologie / Institute for Microbiology
} Bundesanstalt fuer Milchforschung / Federal Dairy Research Centre
} Postfach / P.O. Box 6069, D-24121 Kiel
} Hermann-Weigmann-Str. 1, D-24103 Kiel
} ****************************************************************************
} ****
} Tel. / Phone: +49 (0) 431 609 2343 {} Fax: +49 (0) 431 609 2306
} E-mail: neve-at-bafm.de {} Internet: http://www.bafm.de
} ****************************************************************************
} ****



From daemon Fri Jun 02 16:53:03 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 02 Jun 2000 14:43:49 -0700
Subject: Fwd: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Dear Jim

I spent about two years trying to make good pictures of my NanoGold labeled
protein-DNA complexes. Doing this job I find two main problems: the
sample is unstable under the beam as any biological sample; NanoGold is
much more bright than protein core in dark-field. I find that it is
impossible to record equally perfect signals from NanoGold and protein core
because of short dynamic range for SO-163 film, I believe. I was trying to
make two pictures with different exposure, but it is tricky: in dark-field
mode the automatic exposure meter usually does not work and we have to set
exposure manually, in this case it is difficult to get "right" exposure
time in the right moment, you know. Again, because of sample's short life
under the beam, it is impossible to make a couple pictures at the different
conditions sometime. Keep in mind, please, that to change the film in the
microscope it takes about 10 seconds. Your idea about increasing
signal-noise ratio by collecting more electrons is bright but not
practical. For biological samples (I am talking about non-fixed,
non-stained samples of proteins, DNA or RNA-protein complexes etc) the
electron damage is a huge problem. People are trying to solve it in
different ways. Some using cryo temperature (to stabilize the biological
structure). I was using freeze-drying (I find that freeze-dried samples
are more stable under the beam). But in any case we have deal with very
unstable samples and must to do everything to decrease (not increase as you
recommended) electron dose. Drift is a second big problem for such
application: to increase signal-noise ratio we have to use very thin
support films. Images obtained at such conditions are noisy and in most
cases we have to use image analysis tools to extract the data. It means
that we have to digitize our images anyway. In such situation digital
camera may help. As you, probably, remember I was a person who initiates
this discussion. I think this discussion was very useful for many of us
who are not friendly with digital camera's techniques. We understand the
limitations of the modern digital cameras better now. I would like to say
thank you everybody who was involved in this discussion. There is some
conclusions I make for myself from discussion:


- Film is still cheap and universal material for recording and
storage EM images, sorry CCD.
- CCD TEM camera should not substitute film. Film and camera should
work all together improving the flexibility of the TEM system. For this
reason I will chose side-mount camera if will have money for it.
- For cell-biology (thin sections) where the resolution of the sample
is about 3 nm CCD camera may do a good job allowing users to make a huge
number of pictures (cell-biology guys love it), instantly view and
catalogize them.
- Sometime the digital camera may help in area of high-resolution
(relatively high, guys) EM when image will be digitized anyway. The major
limitation here is small area of view (we need a lot of particles for image
analysis sometime), but you could make the set of overlapping pictures and
digitally combine it. I love, also, Mike Bode idea to make a few very
short exposure pictures and combine it digitally later to reduce noise. The
relatively big size of CCD's pixels is a real problem too.
- CCD camera is expensive "toy". I am not sure that the benefits from
using it will compensate astronomical price, actually the third of the
electron microscope value ($70000 is it 1/3 of microscope's price on
current market?). Currently, I would recognize the CCD TEM camera as funny
"attachment" which may be useful if you rich enough to spend money on it
(it mean, that you have everything else in your EM lab plus some extra
$70000 for the fun playing with digital "toy").
- TEM CCD cameras are under extensive development now. Today's
camera will be replaced on the new model (read better, faster, what else?)
next year. Next year's camera will be easily forgotten next after the next
year and so on... Each new camera will be better that previous one... CCD
TEM camera it is not a good investment of money, I think.
- We should keep in mind that many companies charged extra 3-4K$ for
the installation and training (it is mandatory for Gatan for instance) and
you, probably, have to buy service contract on it even if you have service
contract on the microscope (JEOL's service contract on microscope do not
cover the CCD camera even if you buy it from JEOL).

Best regard, Sergey




} Date: Fri, 02 Jun 2000 21:14:44 +1000
} From: jim {jim-at-proscitech.com.au}
} Subject: RE: Film vs Digital
} To: 'Mike Bode' {mb-at-Soft-Imaging.com} ,
} "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} Cc: "'jim-at-proscitech.com.au'" {jim-at-proscitech.com.au}
} Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Sat Jun 03 08:18:09 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Sat, 03 Jun 2000 14:19:19 +0200
Subject: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

I agree with most of your statements, and I don't think that anybody
would argue the point, that a raw CCD chip has a better resolution that
film. As you pointed out, film can have a very small grain size ( {1
micron) and CCD chips usually have a few microns pixel size.

But that is not the end of the story. An optical system normally
consists of more than a chip or a sheet of film. The question is, can I
get the resolution I want or need. And here the situation is not as
simple. For example: I used to do high-resolution TEM. What you do there
is operate the microscope at optimum condition, then take a picture (on
film). You then go to the darkroom and develop prints by blowing up the
negative 10, 20 or even more times. When you then look at the images,
you can usually see the grains of the film (especially if you then scan
those into a copmputer). So, we are working at the resolution limit of
the film, and according to your postings, we should not be able to see
anything on a CCD. But that's not true. By using some geometrical
properties (the camera sits further down in the column and sees an
already enlarged image) and a tapered fiber-optic, you can acquire just
as good and better images of the same structure. Both images are limited
by the point resolution of the TEM and not by the Film or CCD
resolution.

What I am trying to say, and what I have said before is, that film is
definitely better when it comes to maximizing the product of resolution
AND field of view. However, if we can trade one for the other, I believe
in most cases you get better results from a CCD.

Having said that and looking at a print of Ansel Adams, I am glad there
is film, though!!

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Friday, June 02, 2000 10:16 AM
To: joe fu
Cc: microscopy-at-sparc5.microscopy.com


Date sent: Fri, 02 Jun 2000 11:03:03 -0400
To: c.jeffree-at-ed.ac.uk
} From: joe fu {jofu-at-nist.gov}


Conventional wisdom said that when it came to using TEM for biological
specimens (or beam sensitive specimens) it is always best to use a lower
accelerating voltage. Many lower voltage microscopes (V { 120 kV) were sold
on this assumption.
However is this assumption true? At the present there are many research
establishments, buying TEMs for biological use, who are using 200 to 300 kV
beams. So obviously there has been a shift in the conventional way of
thinking. Being materials based I am not sure what the status quo is in
biological TEM.
I know that the ratio of inelastic to elastic scattering cross sections is
greater than one for the elements Z {12, but how does this change as the
beam energy increases? What are your experiences? This is really just
academic curiosity by the way, but I am sure many of you would appreciate
the question.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Sun Jun 04 10:18:19 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Sat, 03 Jun 2000 11:46:51 -0400
Subject: Sergey's comments Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey writes:

} } - CCD camera is expensive "toy". I am not sure that the
} } benefits from using it will compensate astronomical price, actually
} } the third of the electron microscope value ($70000 is it 1/3 of
} } microscope's price on current market?). Currently, I would
} } recognize the CCD TEM camera as funny "attachment" which may be
} } useful if you rich enough to spend money on it (it mean, that you
} } have everything else in your EM lab plus some extra $70000 for the
} } fun playing with digital "toy").
- TEM CCD cameras are under extensive development now. Today's
camera will be replaced on the new model (read better, faster, what
else?) next year. Next year's camera will be easily forgotten next
after the next year and so on... Each new camera will be better that
previous one... CCD TEM camera it is not a good investment of money,
I think. { {


Sergey:

I appreciate your post, but...


1. Remember that "time is money"...there is no question that there
is a value in the nearly instantaneous result that derives from the
use of digital imaging systems, particular on TEMs. Also, the
ability to rapidly process and analyze your images to determine if
they are "keepers" is priceless, IMO.

2. In our large, national multi-user facility we have been
digital-only since about 1993 or so. We have no darkrooms for plate
loading or enlarging. The only "chemicals" we handle in the entire
photographic process are toner cartridges for our laser printers, or
equivalent media for dye sub printers etc. In most instances, we
process our images electronically all the way through to the final
presentation. This includes preparation of PowerPoint slides for
digital projection for talks, to sending full papers out for
publication on disks or via e-mail attachments. No user has *ever*
complained about the non-availability of film, or about the "lack of
resolution of CCD images" compared to film. On the contrary, we have
users that travel to our laboratory specifically to do work on our
instruments because of the availability of digital imaging, when the
same instruments in their own laboratories are not equipped with
digital cameras.

3. Digital camera systems also provide the capability to conduct
research with outside colleagues live-time via Telepresence
Microscopy methods. This is a rapidly developing capability in our
field that just cannot be done (on a TEM at least) if your
microsocope only uses film for imaging.

4. The 1k x 1k Gatan CCD camera on our Hitachi HF-2000 was purchased
for about $100K in 1993, and was upgraded about 5 years ago to a
multi-scan capability. It is still functioning perfectly today, as
is a similar camera on our JEOL 4000EX TEM. The very newest CCD
cameras might offer faster read-out times, but there has been no
order-of-magnitude improvement in capabilities to make our older
camera so obsolete that we desire to purchase a new one. The point
is that, unlike desk-top computers, the *expensive* digital camera
you purchase today will definitely *not* be obsolete next year...

5. A CCD TEM camera is probably the *best* investment anyone could
make to advance the research throughput in their microscopy facility.

All just MHO...

Larry

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Sun Jun 04 10:18:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sat, 3 Jun 2000 12:53:11 -0500
Subject: Re: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Mike Bode" {mb-at-Soft-Imaging.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} Chris,
}
} I agree with most of your statements, and I don't think that anybody
} would argue the point, that a raw CCD chip has a better resolution that
} film. As you pointed out, film can have a very small grain size ( {1
} micron) and CCD chips usually have a few microns pixel size.
}
} But that is not the end of the story. An optical system normally
} consists of more than a chip or a sheet of film. The question is, can I
} get the resolution I want or need. And here the situation is not as
} simple. For example: I used to do high-resolution TEM. What you do there
} is operate the microscope at optimum condition, then take a picture (on
} film). You then go to the darkroom and develop prints by blowing up the
} negative 10, 20 or even more times. When you then look at the images,
} you can usually see the grains of the film (especially if you then scan
} those into a computer). So, we are working at the resolution limit of
} the film, and according to your postings, we should not be able to see
} anything on a CCD. But that's not true. By using some geometrical
} properties (the camera sits further down in the column and sees an
} already enlarged image) and a tapered fiber-optic, you can acquire just
} as good and better images of the same structure. Both images are limited
} by the point resolution of the TEM and not by the Film or CCD
} resolution.c


For a scanning device be it light, electrons, x-rays or gamma rays is a CCD
array the best way to capture the image. My experience has been with gamma
rays and to some extent x rays. We have not found a good way to focus gamma
rays so we use a crystal that emitted light and a photomultiplier tube and
we
got good images. The resolution depended on the aperture of the gamma ray
source and was pretty large.

If you are scanning a sample wiht an electron beam the same principle should
work. You would not need long dwell times but build the images out of
multiple
scans.

If you are scanning the sample the array of pixels seems redundant to me.
Also a photomultiplier tube and crystal are a great deal more sensitive than
CCD arrays and have a good deal more dynamic range.

The time to make a film image is allows going to be less than making a
digital image.
The ability to immediately see the digital image is a very handy thing.
There are ways
to develop B&W film that you can see your image in 5 minutes. For 4 X 5
images I am
using BZT tubes that are tubs with a inter circumference of a little over 4
inches. You
put the file and developer in the tube and put the tub in a pan of water and
spin the tube
in the water. The stop bath and fixing can be carried out in room light and
it results in
the most even development I have ever seen.

For archival storage I don't think 35 mm film can be beat for cost and
resolution. Unfortunately
you can't see the results until later occasional making it necessary to
reshoot the session if
you rely on film alone.

If you really need long term archival storage you might consider using a
slide printer
for a computer to print the digital images. We know the life of silver film
is longer then
100 years. For the LM folks that are using color images you could use
PhotoShop to
do a color separation and print out three negatives on black and white for
storage.

Hard drives And CD ROMS have come a long way but I loose about 20% of my
hard
drive storage a year and loose an occasional CDROM to scratches. Film would
survive
the scratches with a minimal loss of information insted loosing the whole
CDROM.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00






From daemon Sun Jun 04 10:18:27 2000



From: STANSMAN-at-aol.com
Date: Sat, 3 Jun 2000 17:21:51 EDT
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Please be aware that this is a commercial post that may be of interest to
some of the list members.


Digital light microscope cameras are now available that use a highly touted
CCD detector: Color or monochrome; fast interline transfer, high sensitivity,
low noise; 6.7 micron square pixel; 2/3 inch 1.3 Million chip; PCI bus frame
transfer. This detector is ideal to use in light microscopy applications.
Now precision technology and fast high capacity cheap computers allow this
detector to be "Micro Stepped" providing digital images of up to 12 million
pixels to be captured very quickly and providing file sizes of up to 35 MB in
24 bit color images. "Inter Pixel Stepping" technology will allow a
considerably less expensive approach to digital imaging that can now approach
film resolving capability in a light microscope.

Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
light microscopy applications. With high pixel density capable digital
cameras, now lower magnification, low NA (lower resolving power) objectives
can be used to acquire matching digital resolution images of wide fields of
view.

Information on Nikon's Instrument Division "Digital Eclipse" family of
digital cameras for microscopy including the new DXM1200 digital color camera
will soon be available on our web site www.nikonusa.com or you can go to
our Dealer Locator at
http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
local Nikon authorized microscope dealer for further information.

Best Regards,

Stan Schwartz
Manager, BioSciences Dept.
Nikon Inc Instrument Division
1300 Walt Whitman Rd.
Melville, NY 11747
631-547-8500
631-547-4033 Fax
Schwartz-at-nikonincmail.com
www.nikonusa.com


Earlier post
=============================================================
Geoff
Analysing this a little further:
A 25 Mbyte image = 8.3 M pixels to cover 24x36mm or 864mm^2
this represents 98.2 pixels per mm, or 49 line pairs per mm. Pixel
size 10.2 um. Resolution about 50 line pairs per mm at best.

A 25M pixels image used to capture a 24x36mm Kodachrome
slide represents 28,935 pixels per mm^2 or 170 pixels per mm.
This is equivalent to a maximum resolution of 85 line pairs per mm,
which may be on the conservative side for Kodachrome. pixel size
= 5.88um. The image will be approx. 6120x4082 pixels, generating
a file size of approx 75Mb for a 24-bit (8+8+8bit) RGB image.

To record 120 line pairs per mm, which many top 35mm camera
lenses can achieve, a minimum of 240 pixels per mm are required,
each 4.2 um wide. This equates to 8640x5760 pixels for a
24x36mm frame = 50M-pixels or 50Mb in 8-bit greyscale, or 150Mb
in 24-bit RGB.

At 320 line pairs per mm (Technical Pan) the minimum required
640 pixels per mm is a pixel size of 1.56 um

Presumably for a light image the diffraction limited resolution is
approx 1/2 lambda which at 540nm is 0.27um.
So looking to the future of ultimate-performance CCDs, direct
recording of a diffraction limited light image projected onto the
sensor requires at the very least 3703 pixels per image mm or
13,717,421 pixels per mm^2 (greyscale 8-bit)

However, if we are doing light microscopy with an NA 1.4 x100
objective, how much resolving power do we need on CCD or film?

Data is at 0.27um resolution (lambda = 540nm). Let's round this to
0.3um. Magnification at 24x36mm film image is x100, so pixels
must be an absolute maximum of 30um wide to record the
significant data = 33.3 pixels per mm, equivalent to 800x1200
pixels to record the whole 35mm negative area. However, most
CCDs are much smaller than 35 mm frames, typically 1/3 inch. So
an 800x1200 pixel CCD at 8x12 mm, 1/3 of the linear dimensions
of a 35mm frame would use 9 pixels to record the smallest image
details. This is about right from the point of view of resolution, but
to record the whole 35mm frame we need about 2400x3600 pixels
on our CCD.

Note also that Technical Pan has (depending on the criterion used
to assess its performance) up to 10 times the resolving power
required to record all there is to see in a diffraction-limited LM
image made with a 100x NA 1.4 lens. So you can comfortably
afford to use a 60x NA 1.4 lens, thereby getting the same
resolution with a bigger field of view.

Many years ago, I took a photograph of a street scene using a
Canon 35mm SLR loaded with Kodak Recordak (I think this was a
single layer microfilm emulsion). Examined in a light microscope,
the image clearly, legibly recorded the brand-name of a child's
push chair. I tried to print this brand name using a DeVere point-
source enlarger with an image size of 20x30 inches produced with
a Schneider Componon lens, but was completely unable to
produce a legible image. The point I am making here is that the
combination of some high performance films, with high quality
lenses of the standard produced by the leading camera
manufacturers can record more detail on the film than you can
easily get back out by conventional printing. I suspect the same is
true of EM exposures.

So there is no contest - film beats CCDs for resolution hands
down. And you can process the image on the cheap. No money
goes to Intel or Microscoft, Adobe or Epson. But resolution is not
primarily what we buy CCDs for. We buy them primarily for instant
image capture in a format suitable for digital storage, digital
transmission quantitative data recording and image processing.

Chris

=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Sun Jun 04 10:18:28 2000



From: A.P.Alves de Matos :      apmatos-at-ip.pt
Date: Sat, 3 Jun 2000 22:25:13 +0100
Subject: RE: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I work with EPON sections of biological specimens. It seems clear to me that
60KV introduces more dammage than 80Kv. The sections are more unstable and
tend to break more easily at the lower voltage.

Dr. A.P. Alves de Matos
Pathology Department
Curry Cabral Hospital
Lisbon




------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Conventional wisdom said that when it came to using TEM for biological
specimens (or beam sensitive specimens) it is always best to use a lower
accelerating voltage. Many lower voltage microscopes (V { 120 kV) were sold
on this assumption.
However is this assumption true? At the present there are many research
establishments, buying TEMs for biological use, who are using 200 to 300 kV
beams. So obviously there has been a shift in the conventional way of
thinking. Being materials based I am not sure what the status quo is in
biological TEM.
I know that the ratio of inelastic to elastic scattering cross sections is
greater than one for the elements Z {12, but how does this change as the
beam energy increases? What are your experiences? This is really just
academic curiosity by the way, but I am sure many of you would appreciate
the question.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************






From daemon Sun Jun 04 10:18:41 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 03 Jun 2000 19:17:50 -0700
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 02:21 PM 6/3/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I hope not.


} Digital light microscope cameras are now available that use a highly touted
} CCD detector: Color or monochrome; fast interline transfer, high sensitivity,
} low noise; 6.7 micron square pixel; 2/3 inch 1.3 Million chip; PCI bus frame
} transfer. This detector is ideal to use in light microscopy applications.
} Now precision technology and fast high capacity cheap computers allow this
} detector to be "Micro Stepped" providing digital images of up to 12 million
} pixels to be captured very quickly and providing file sizes of up to 35 MB in
} 24 bit color images. "Inter Pixel Stepping" technology will allow a
} considerably less expensive approach to digital imaging that can now approach
} film resolving capability in a light microscope.
}
} Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
} light microscopy applications. With high pixel density capable digital
} cameras, now lower magnification, low NA (lower resolving power) objectives
} can be used to acquire matching digital resolution images of wide fields of
} view.
}
} Information on Nikon's Instrument Division "Digital Eclipse" family of
} digital cameras for microscopy including the new DXM1200 digital color camera
} will soon be available on our web site www.nikonusa.com or you can go to
} our Dealer Locator at
} http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
} local Nikon authorized microscope dealer for further information.
}
} Best Regards,
}
} Stan Schwartz
} Manager, BioSciences Dept.
} Nikon Inc Instrument Division
} 1300 Walt Whitman Rd.
} Melville, NY 11747
} 631-547-8500
} 631-547-4033 Fax
} Schwartz-at-nikonincmail.com
} www.nikonusa.com

As a long term past user of Nikon equipment, it is a tale of sorrow.
Most recently, the digital E1 and E2 are dismal failures. Consumers
are sending back 990's in droves (see rec.photo.marketplace.digital).
I really think that Nikon blew it in regards to digicams. How Nikon can
claim to get a 1.3M pixel imager to produce realistic 12M pixel images
is rather absurd....if not offensive. The most recent D1 is a joke. Albeit,
an expensive one.

Nikon blew it in the scanner arena and made huge blunders in high end
pro digicams (E1 & E2). Total junk from my personal experiences. I am
not at all prone to spend a dime on any new Nikon digital things. In fact,
I have
dumped my Nikon so-called pro lenses and bodies for Contax. But this
is another story.

My advice is to be very wary....be very wary of Nikon. I do not use Nikon
cameras anymore and I do not use Nikon microscopes anymore.
But it is your money and your decision. As they say, "Caveat emptor."

gg

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Jun 04 10:19:00 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Sun, 4 Jun 2000 09:49:36 +0100
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rubbish! With all due respect this is a complete distortion and mis-
use of the point I made. To realise this objective with low NA lenses
you would have to find some way to defeat the laws of physics. No
CCD imager, however clever will make it possible to correct the
shortcomings of cheap, low-performance optics. You clearly
misunderstood the point, which is that microscope lens resoltuion is
not fundamentally dependent on the magnification factor but on the
numerical aperture, which also determines resolution. A x100 NA
1.4 lens resolves no more detail than a x60 NA 1.4, but simply
magnifies the image further. This is "empty magnification", which is
only useful if your image sensor (film or CCD) has limited resolving
power. With a very high resolution film like Technical Pan, you can
take advantage of this fact to capture a wide-field diffraction-limited
image with a x60 1.4 lens. That is not an option with a low NA lens
irrespective of the properties of the sensor, CCD or otherwise.

} Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
} light microscopy applications. With high pixel density capable digital
} cameras, now lower magnification, low NA (lower resolving power) objectives
} can be used to acquire matching digital resolution images of wide fields of
} view.
}
} Information on Nikon's Instrument Division "Digital Eclipse" family of
} digital cameras for microscopy including the new DXM1200 digital color camera
} will soon be available on our web site www.nikonusa.com or you can go to
} our Dealer Locator at
} http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
} local Nikon authorized microscope dealer for further information.
}
} Best Regards,
}
} Stan Schwartz
} Manager, BioSciences Dept.
} Nikon Inc Instrument Division
} 1300 Walt Whitman Rd.
} Melville, NY 11747
} 631-547-8500
} 631-547-4033 Fax
} Schwartz-at-nikonincmail.com
} www.nikonusa.com
}
}
} Earlier post
} =============================================================
} Geoff
} Analysing this a little further:
} A 25 Mbyte image = 8.3 M pixels to cover 24x36mm or 864mm^2
} this represents 98.2 pixels per mm, or 49 line pairs per mm. Pixel
} size 10.2 um. Resolution about 50 line pairs per mm at best.
}
} A 25M pixels image used to capture a 24x36mm Kodachrome
} slide represents 28,935 pixels per mm^2 or 170 pixels per mm.
} This is equivalent to a maximum resolution of 85 line pairs per mm,
} which may be on the conservative side for Kodachrome. pixel size
} = 5.88um. The image will be approx. 6120x4082 pixels, generating
} a file size of approx 75Mb for a 24-bit (8+8+8bit) RGB image.
}
} To record 120 line pairs per mm, which many top 35mm camera
} lenses can achieve, a minimum of 240 pixels per mm are required,
} each 4.2 um wide. This equates to 8640x5760 pixels for a
} 24x36mm frame = 50M-pixels or 50Mb in 8-bit greyscale, or 150Mb
} in 24-bit RGB.
}
} At 320 line pairs per mm (Technical Pan) the minimum required
} 640 pixels per mm is a pixel size of 1.56 um
}
} Presumably for a light image the diffraction limited resolution is
} approx 1/2 lambda which at 540nm is 0.27um.
} So looking to the future of ultimate-performance CCDs, direct
} recording of a diffraction limited light image projected onto the
} sensor requires at the very least 3703 pixels per image mm or
} 13,717,421 pixels per mm^2 (greyscale 8-bit)
}
} However, if we are doing light microscopy with an NA 1.4 x100
} objective, how much resolving power do we need on CCD or film?
}
} Data is at 0.27um resolution (lambda = 540nm). Let's round this to
} 0.3um. Magnification at 24x36mm film image is x100, so pixels
} must be an absolute maximum of 30um wide to record the
} significant data = 33.3 pixels per mm, equivalent to 800x1200
} pixels to record the whole 35mm negative area. However, most
} CCDs are much smaller than 35 mm frames, typically 1/3 inch. So
} an 800x1200 pixel CCD at 8x12 mm, 1/3 of the linear dimensions
} of a 35mm frame would use 9 pixels to record the smallest image
} details. This is about right from the point of view of resolution, but
} to record the whole 35mm frame we need about 2400x3600 pixels
} on our CCD.
}
} Note also that Technical Pan has (depending on the criterion used
} to assess its performance) up to 10 times the resolving power
} required to record all there is to see in a diffraction-limited LM
} image made with a 100x NA 1.4 lens. So you can comfortably
} afford to use a 60x NA 1.4 lens, thereby getting the same
} resolution with a bigger field of view.
}
} Many years ago, I took a photograph of a street scene using a
} Canon 35mm SLR loaded with Kodak Recordak (I think this was a
} single layer microfilm emulsion). Examined in a light microscope,
} the image clearly, legibly recorded the brand-name of a child's
} push chair. I tried to print this brand name using a DeVere point-
} source enlarger with an image size of 20x30 inches produced with
} a Schneider Componon lens, but was completely unable to
} produce a legible image. The point I am making here is that the
} combination of some high performance films, with high quality
} lenses of the standard produced by the leading camera
} manufacturers can record more detail on the film than you can
} easily get back out by conventional printing. I suspect the same is
} true of EM exposures.
}
} So there is no contest - film beats CCDs for resolution hands
} down. And you can process the image on the cheap. No money
} goes to Intel or Microscoft, Adobe or Epson. But resolution is not
} primarily what we buy CCDs for. We buy them primarily for instant
} image capture in a format suitable for digital storage, digital
} transmission quantitative data recording and image processing.
}
} Chris
}
} =====================================================================
} DR CHRIS JEFFREE
} BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} UNIVERSITY OF EDINBURGH
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 131 650 5345
} FAX. #44 131 650 6563
} Mobile 0410 585 401
} email c.jeffree-at-ed.ac.uk
} SEM / TEM bookings sem-at-ed.ac.uk
} =====================================================================
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Jun 04 10:19:08 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 4 Jun 2000 21:20:44 +1000
Subject: RE: Film vs Digital/ switched to EM techn

Contents Retrieved from Microscopy Listserver Archives
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Lets place the exhausted and exhausting Digital topic aside.
Sergey, you do have a challenging project and one that is worth discussing.
Just maybe somebody has an idea that will help you. I think that we discuss in
this forum pixels too much and microscopy too little.

You probably have tried most of my following suggestions, but just incase, here
are a couple of my thoughts:

Dark field contrast is enhanced most when the density between specimen and
background is greatest. So its most effective with no specimen support. You
could try your luck with the superfine mesh grids now available; these thin bar
grids can be purchased down to 2000 mesh and these grids have a 7.5um hole
size. If that is not a fine enough support, then try holey films with a
net-like structure.

Carbon coating will stabilize samples dramatically. A little loss of contrast
is inevitable, but a least carbon on the grid or holey plastic film does not
matter.

Don't see why you want to render the gold nano particles with detail (I assume
some greys). In TEM I would expect gold above about 5nm to be black. In STEM or
FESEM you could get greys easily, but you may not get the resolution required.

Increasing if available to 200 or more the kV, will give more brightness, less
contrast, better resolution (specimen dependent). Most importantly, specimen
damage frequently is less at higher kV, that depends on the specimen again. I
think that damage is less in specimens with greater electron transparency, but
one of our "physical" gurus may explain the whys and wherefores.

Without switching film-types, you can develop the TEM films in things like
Microdol-X, Microphen or Rodinal (hope those developers still exist). You could
also use D-19 more dilute than normal. For most EM purposes all of these would
give too softer negatives, but they just may suit you. Over developing in light
photography yields more contrast - not so in TEM, where more electrons are the
main contrast mechanism. Hence slower film and denser negatives are preferred,
especially by most biologists.

I think that we started out with too much contrast, but a grainy image because
of insufficient electron exposure. Hmmm, I think that we should take a holiday.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 7:44 AM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Dear Jim
}
} I spent about two years trying to make good pictures of my NanoGold labeled
} protein-DNA complexes. Doing this job I find two main problems: the
} sample is unstable under the beam as any biological sample; NanoGold is
} much more bright than protein core in dark-field. I find that it is
} impossible to record equally perfect signals from NanoGold and protein core
} because of short dynamic range for SO-163 film, I believe. I was trying to
} make two pictures with different exposure, but it is tricky: in dark-field
} mode the automatic exposure meter usually does not work and we have to set
} exposure manually, in this case it is difficult to get "right" exposure
} time in the right moment, you know. Again, because of sample's short life
} under the beam, it is impossible to make a couple pictures at the different
} conditions sometime. Keep in mind, please, that to change the film in the
} microscope it takes about 10 seconds. Your idea about increasing
} signal-noise ratio by collecting more electrons is bright but not
} practical. For biological samples (I am talking about non-fixed,
} non-stained samples of proteins, DNA or RNA-protein complexes etc) the
} electron damage is a huge problem. People are trying to solve it in
} different ways. Some using cryo temperature (to stabilize the biological
} structure). I was using freeze-drying (I find that freeze-dried samples
} are more stable under the beam). But in any case we have deal with very
} unstable samples and must to do everything to decrease (not increase as you
} recommended) electron dose. Drift is a second big problem for such
} application: to increase signal-noise ratio we have to use very thin
} support films. Images obtained at such conditions are noisy and in most
} cases we have to use image analysis tools to extract the data. It means
} that we have to digitize our images anyway. In such situation digital
} camera may help. As you, probably, remember I was a person who initiates
} this discussion. I think this discussion was very useful for many of us
} who are not friendly with digital camera's techniques. We understand the
} limitations of the modern digital cameras better now. I would like to say
} thank you everybody who was involved in this discussion. There is some
} conclusions I make for myself from discussion:
}
}
} - Film is still cheap and universal material for recording and
} storage EM images, sorry CCD.
} - CCD TEM camera should not substitute film. Film and camera should
} work all together improving the flexibility of the TEM system. For this
} reason I will chose side-mount camera if will have money for it.
} - For cell-biology (thin sections) where the resolution of the sample
} is about 3 nm CCD camera may do a good job allowing users to make a huge
} number of pictures (cell-biology guys love it), instantly view and
} catalogize them.
} - Sometime the digital camera may help in area of high-resolution
} (relatively high, guys) EM when image will be digitized anyway. The major
} limitation here is small area of view (we need a lot of particles for image
} analysis sometime), but you could make the set of overlapping pictures and
} digitally combine it. I love, also, Mike Bode idea to make a few very
} short exposure pictures and combine it digitally later to reduce noise. The
} relatively big size of CCD's pixels is a real problem too.
} - CCD camera is expensive "toy". I am not sure that the benefits from
} using it will compensate astronomical price, actually the third of the
} electron microscope value ($70000 is it 1/3 of microscope's price on
} current market?). Currently, I would recognize the CCD TEM camera as funny
} "attachment" which may be useful if you rich enough to spend money on it
} (it mean, that you have everything else in your EM lab plus some extra
} $70000 for the fun playing with digital "toy").
} - TEM CCD cameras are under extensive development now. Today's
} camera will be replaced on the new model (read better, faster, what else?)
} next year. Next year's camera will be easily forgotten next after the next
} year and so on... Each new camera will be better that previous one... CCD
} TEM camera it is not a good investment of money, I think.
} - We should keep in mind that many companies charged extra 3-4K$ for
} the installation and training (it is mandatory for Gatan for instance) and
} you, probably, have to buy service contract on it even if you have service
} contract on the microscope (JEOL's service contract on microscope do not
} cover the CCD camera even if you buy it from JEOL).
}
} Best regard, Sergey
}
}
}
}
} } Date: Fri, 02 Jun 2000 21:14:44 +1000
} } From: jim {jim-at-proscitech.com.au}
} } Subject: RE: Film vs Digital
} } To: 'Mike Bode' {mb-at-Soft-Imaging.com} ,
} } "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} } Cc: "'jim-at-proscitech.com.au'" {jim-at-proscitech.com.au}
} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} } Organization: ProSciTech
} } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } The world is full of possible solutions, but are they practical.?
} } To produce high-resolution, dark-field or any others TEM images that require
} } more electrons to form a clear image, Mike Bode would use multiple digital
} } exposures. The exposures could be layered and combined into one superior
} } image.
} } This image would be made up of more pixel and is formed by more electrons
} } and
} } so would be noise-free and hence could be further enlarged then otherwise
} } possible. Perhaps.
} } Beam blanking would largely save the specimen from beam damage and drift
} } could
} } be compensated for by matching up the digitals. Great.
} } How much time is required between exposures to transfer a minimum 10mb image
} } per exposure? What would be the total time from focusing to the last
} } exposure?
} } What about Z-drift in the interim requiring objective changes and what about
} } the total cost of this additional get-up. The mind boggles at a through
} } focus
} } series.
} } When pushing the limits a piece of film seems more effective, cheaper and fa
} } ster.
} } Again, I don't doubt that there is now a large place for digital in TEM, but
} } its no panacea.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
wrote:
} }
} } }
} } } No, it's not really a problem. It's been done with low density
} } } microscopy all the time. Granted, there are some technical aspects to be
} } } overcome, but (and I can only speak for ourselves) we have done that on
} } } a number of microscopes. You are of course correct, that 10 images at
} } } 0.8 seconds take longer than 8 seconds as the image has to be
} } } transferred, etc. BUT: that's what beam blankers are for. It is pretty
} } } straightforward to take an image at 0.8 seconds, then blank the beam
} } } very quickly before taking the next image. That way you get pretty close
} } } to the 8 sec total exposure. If there is no beam blanker on the
} } } microscope, in most cases it can be added.
} } }
} } } I am not sure what you mean by "too sensitive". The cameras are usually
} } } constructed so that 1 electron from the beam creates between a few tenth
} } } to a few counts (these are all statistical data, of course). The well
} } } width divided by this sensitivity then determines, how many primary
} } } electrons are needed to fully expose one pixel. For example, if the well
} } } width is 50,000 electrons and the sensitivity is 1 count/electron, one
} } } needs 50,000 primary electrons to fill the well. This translates into
} } } roughly a 0.4% statistical error.
} } }
} } } } From a practical standpoint: You can take images with most cameras when
} } } the exposure meter on the microscope reads a couple of seconds without
} } } overexposing the camera. On the other hand, you can reduce the intensity
} } } of the beam until you see single electron events.
} } }
} } } The one area where CCD cameras may be too sensitive is diffraction. The
} } } normally huge intensity in the transmitted beam often leads to
} } } saturation. In CCDs this can lead to blooming (the intensity spills over
} } } into neighboring pixels). This can be taken care of with special chips
} } } that have anti-blooming features, but this usually has some other
} } } drawbacks. Again, this can also be overcome somewhat with multiple
} } } exposures. Film behaves more civilized here, as it simply stops
} } } responding to the electrons, but this makes film more or less useless
} } } for quantitative measurements of diffraction patterns. I have done
} } } diffraction with CCDs many times and though it does require some
} } } tweaking, one can get very good results from them.
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } }
} } } -----Original Message-----
} } } From: jim [mailto:jim-at-proscitech.com.au]
} } } Sent: Wednesday, May 31, 2000 9:37 PM
} } } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } } Subject: RE: Film vs Digital
} } }
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Yes, it could be done "in theory". Somebody would need to figure out the
} } }
} } } software and perhaps modify the hardware. Then we would find that the
} } } total
} } } exposure of the specimen to the electron beam maybe a muliple of the
} } } film's
} } } exposure. Afterall, an 8 sec film exposure would not amount in digital
} } } to
} } } 10x0.8, but we would require considerable time in between exposures.
} } } Since the
} } } problems in the discussed circumstances are specimen movement and beam
} } } damage,
} } } it seems that taking multiple exposures is a poor option.
} } }
} } } Digital cameras are for some situation too sensitive to electron
} } } exposure.
} } } Cutting back on electrons is no option since its the electrons that form
} } } the
} } } image in the first instance.
} } } Much easier in light microscopy . . . insert a neutral density filter.
} } } Cheers
} } } Jim Darley
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } } www.proscitech.com
} } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}



From daemon Sun Jun 04 10:19:08 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 4 Jun 2000 18:33:42 +1000
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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I have two replies and Mike my well have four replies to these.
1. There has been a parallel discussion concerning resolution of film versus
digital images. Clearly in raw power digital cannot compete since film has
multi gigabyte capacity. I added to that thread that what matters is: does
digital have enough power and that frequently it would. Mike reinforced and
strengthened that argument, finishing with the note that he is glad for film
when looking at prints by Ansel Adams.
(Ansel Adams until about 30 years ago carted for decades large format cameras
through US National Parks, especially Yosemite, producing fantastic landscape
photographs and books)
Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet film,
probably rated at 400 ISO. The line resolution of such prints much exceeds our
eyes' resolution, but still results in superior gradation and detail. TEM film,
even when much enlarged has such details too.
Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM, and
because of the limited enlargability of light microscopy (concrete ceiling due
to wavelengths) it's reasonable, but minimal for light microscopy. TEM can do
and deserves better.

Incidentally, from the outset I cited increased "enlargability" to obtain high
resolution TEM, as one of films major advantages, since greater depths of field
at moderate powers makes high powers through photo enlarging a desirable
technique. The small additional magnification yielded by placing a digital
camera lower in the column does not compensate. So greater "enlargability" of
digitals would be desirable, but is limited by pixel size.

2 The thread was initiated by Sergey. He had problems visualising certain
specimens in dark field. The use of his "beaut" digital TEM camera made things
worse. I pointed out that the shorter exposure reduced the number of electrons
forming the image, hence more noise. I believe that a good part of Mike's case
will be settled in his favour when we hear "Eureka" from Sergey's lab. I don't
doubt that much more can be done with digital now and that further improvements
are on the way. Mike's "solution" may well be possible, but I don't believe its
a snap; in any case: Show Sergey!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com] wrote:
}
} Well, let's see:
}
} Jim wrote:
}
} Mike Bode would use multiple digital
} exposures. The exposures could be layered and combined into one superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
}
} No, I did not talk about further enlargements. All I wanted to say is,
} that a more noise-free image can be achieved by adding multiple images,
} and that this also to some extent helps with drift of the sample during
} acquisition.
}
} Jim wrote:
}
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure?
}
} How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} information is about 2.5 MB (uncompressed). We acquire about 10 of those
} per second and transfer them across the PC bus to the display. Putting
} them on them into Memory might add a few tenth of a second. Writing to
} HD can be done after all images are acquired.
}
} Jim wrote:
}
} What would be the total time from focusing to the last exposure?
} What about Z-drift in the interim requiring objective changes
}
} Why would we have to worry about that, if we don't have to worry about
} that when taking the image on film? In fact, we could take care of this
} by looking at the image between exposures and correct for z-drift.
} However, as you said, that would add to the overall time and exposure. I
} was comparing a normal dark field image taken on film at 8 seconds with
} acquiring the same image on a "too sensitive" CCD camera by adding up 10
} consecutive .8 second images. Why would the sample drift (in x, y or z)
} substantially more in 8+delta seconds than in 8?
}
} Jim wrote:
}
} what about the total cost of this additional get-up
}
} That of course depends on the microscope and there is no general answer.
} For example on a LEO 912 I believe the blanker is standard. The
} additional cost to use an acquisition scheme like this with our software
} is $0 plus perhaps a bit of time to write a small macro. On other
} microscopes one might have to add a beam blanker and perhaps a control
} mechanism for the beam blanker. But I would guess, that this cost is not
} very high. All modern microscopes are computer controller anyway, so it
} is most likely just a control command that needs to be sent to the
} microscope over a serial port if the beam blanker is installed. Piece of
} cake.
}
} Jim wrote:
}
} The mind boggles at a through focus series.
}
} You're right here. But I don't think we were talking about through-focus
} series. Incidentally, we do through-focus series on light microscopes
} and reconstruction routinely. Takes a few images at different focus (or
} for a light microscope: stage) settings. The rest is done off-line.
} Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} agree that TEM is different here and much more complicated due to the
} complicated Contrast Transfer Function. However, this could in principle
} be sorted out.
}
} Jim wrote:
}
} Again, I don't doubt that there is now a large place for digital in TEM,
} but
} its no panacea.
}
} I also agree with you on that one. But using the additional computer
} possibilities of digital imaging might take you further than expected.
}
} Michael
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Friday, June 02, 2000 5:15 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'jim-at-proscitech.com.au'
} Subject: RE: Film vs Digital
}
}
} The world is full of possible solutions, but are they practical.?
} To produce high-resolution, dark-field or any others TEM images that
} require
} more electrons to form a clear image, Mike Bode would use multiple
} digital
} exposures. The exposures could be layered and combined into one superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
} Beam blanking would largely save the specimen from beam damage and drift
} could
} be compensated for by matching up the digitals. Great.
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure? What would be the total time from focusing to the last
} exposure?
} What about Z-drift in the interim requiring objective changes and what
} about
} the total cost of this additional get-up. The mind boggles at a through
} focus
} series.
} When pushing the limits a piece of film seems more effective, cheaper
} and fa
} ster.
} Again, I don't doubt that there is now a large place for digital in TEM,
} but
} its no panacea.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} wrote:
} }
} } No, it's not really a problem. It's been done with low density
} } microscopy all the time. Granted, there are some technical aspects to
} be
} } overcome, but (and I can only speak for ourselves) we have done that
} on
} } a number of microscopes. You are of course correct, that 10 images at
} } 0.8 seconds take longer than 8 seconds as the image has to be
} } transferred, etc. BUT: that's what beam blankers are for. It is pretty
} } straightforward to take an image at 0.8 seconds, then blank the beam
} } very quickly before taking the next image. That way you get pretty
} close
} } to the 8 sec total exposure. If there is no beam blanker on the
} } microscope, in most cases it can be added.
} }
} } I am not sure what you mean by "too sensitive". The cameras are
} usually
} } constructed so that 1 electron from the beam creates between a few
} tenth
} } to a few counts (these are all statistical data, of course). The well
} } width divided by this sensitivity then determines, how many primary
} } electrons are needed to fully expose one pixel. For example, if the
} well
} } width is 50,000 electrons and the sensitivity is 1 count/electron, one
} } needs 50,000 primary electrons to fill the well. This translates into
} } roughly a 0.4% statistical error.
} }
} } } From a practical standpoint: You can take images with most cameras
} when
} } the exposure meter on the microscope reads a couple of seconds without
} } overexposing the camera. On the other hand, you can reduce the
} intensity
} } of the beam until you see single electron events.
} }
} } The one area where CCD cameras may be too sensitive is diffraction.
} The
} } normally huge intensity in the transmitted beam often leads to
} } saturation. In CCDs this can lead to blooming (the intensity spills
} over
} } into neighboring pixels). This can be taken care of with special chips
} } that have anti-blooming features, but this usually has some other
} } drawbacks. Again, this can also be overcome somewhat with multiple
} } exposures. Film behaves more civilized here, as it simply stops
} } responding to the electrons, but this makes film more or less useless
} } for quantitative measurements of diffraction patterns. I have done
} } diffraction with CCDs many times and though it does require some
} } tweaking, one can get very good results from them.
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Wednesday, May 31, 2000 9:37 PM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: RE: Film vs Digital
} }
} }
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Yes, it could be done "in theory". Somebody would need to figure out
} the
} }
} } software and perhaps modify the hardware. Then we would find that the
} } total
} } exposure of the specimen to the electron beam maybe a muliple of the
} } film's
} } exposure. Afterall, an 8 sec film exposure would not amount in digital
} } to
} } 10x0.8, but we would require considerable time in between exposures.
} } Since the
} } problems in the discussed circumstances are specimen movement and beam
} } damage,
} } it seems that taking multiple exposures is a poor option.
} }
} } Digital cameras are for some situation too sensitive to electron
} } exposure.
} } Cutting back on electrons is no option since its the electrons that
} form
} } the
} } image in the first instance.
} } Much easier in light microscopy . . . insert a neutral density
} filter.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }



From daemon Sun Jun 04 10:19:09 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Sun, 04 Jun 2000 09:53:36 -0400
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
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****This is a commercial response from a Vendor****

Gary and List,

I am sorry you have had a bad experience with Nikon products. However, you are
entitled to your "opinion" and therefore; I am entitled to a response. First, the
new Digital Eclipse Camera capable of 12M pixels using a stepper mode is exactly
the same technology that the new Zeiss Axiocam and an Olympus model uses which has
been very well received by the microscope community.

Next, the successor to E1, E2 (technically, made by Fuji) is the current Nikon D1
(Digital SLR) was NOT intended for microscopes. It CAN go on one, but is limited
to Brightfield applications and has no NTSC (video) out for focusing. However, it
is by far the standard in Digital Cameras! It has won every Journal Award in it's
field and is the definitive choice for Professional Photo-Journalists, including
the massive market share Nikon has and the majority of Pulitzer Prize winners. As
for Film Scanner products, the Nikon Coolscan line is still the standard in the
industry. Especially, high end units with Auto Feeders. All Nikon digital products
are not perfect. However, these are technical products that are evolving 6 months
at a time and are truly in their infantile state from where they will be in just a
few years from now! I would prefer to address your specific problems, but you gave
none and just called it "junk". With that mentality, I feel my Pentium 266
computer is "junk", but I do not blame the manufacturer because I own an older
model.

As for the Nikon Coolpix line (990), pardon my sarcasm, but where are "all the
returns" because we need them to fulfill the 38,000 unit Backorders! This camera
is so wildly successful EVERY Dealer (Photo and Microscope) is begging for them.
Check out Ebay for example; the cameras are selling for more than List Price.
Sound to me like a success if you understand the basics of Economics and Supply
and Demand. Is it the perfect microscope camera? No, I don't even think so...but
it is the ONLY high resolution (3.34M) digital camera, with live video out, goes
on any brand or model microscope for under $1000. Oh, did I forget to mention it
also won almost every Magazine Award in its class (not all, it actually received a
tie with the Olympus 3030 in one, which is a very nice camera, but does not mount
a microscope).

Finally, I have been on this List for many years and have respected the use and
rules of this forum. We Vendors for the most part are respectful of the opinions
of our users. However, making a "blanket statement" like "stay away from Nikon",
does not serve the public well; NOR YOURSELF! If you or any customer has specific
problems; we want to know about it; as does any reputable manufacturer for future
product improvement. The fact is Nikon has the largest market share in microscopes
and professional camera equipment and is climbing very quickly on the digital
camera list too. So, in short you are entitled to your opinion, but the rest of
the world by far does NOT agree with it............

I personally apologize to any members this correspondence offends. Believe me, my
preference is to serve the list and answer technical microscopy questions as I
have for many years, but this unfair and libel attack required a response.

Regretfully,

Lawrence Kordon
Nikon, Inc.
Senior Bioscience Specialist
nikon-at-jagunet.com

"Dr. Gary Gaugler" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 02:21 PM 6/3/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello all,
} }
} } Please be aware that this is a commercial post that may be of interest to
} } some of the list members.
}
} I hope not.
}
} } Digital light microscope cameras are now available that use a highly touted
} } CCD detector: Color or monochrome; fast interline transfer, high sensitivity,
} } low noise; 6.7 micron square pixel; 2/3 inch 1.3 Million chip; PCI bus frame
} } transfer. This detector is ideal to use in light microscopy applications.
} } Now precision technology and fast high capacity cheap computers allow this
} } detector to be "Micro Stepped" providing digital images of up to 12 million
} } pixels to be captured very quickly and providing file sizes of up to 35 MB in
} } 24 bit color images. "Inter Pixel Stepping" technology will allow a
} } considerably less expensive approach to digital imaging that can now approach
} } film resolving capability in a light microscope.
} }
} } Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
} } light microscopy applications. With high pixel density capable digital
} } cameras, now lower magnification, low NA (lower resolving power) objectives
} } can be used to acquire matching digital resolution images of wide fields of
} } view.
} }
} } Information on Nikon's Instrument Division "Digital Eclipse" family of
} } digital cameras for microscopy including the new DXM1200 digital color camera
} } will soon be available on our web site www.nikonusa.com or you can go to
} } our Dealer Locator at
} } http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
} } local Nikon authorized microscope dealer for further information.
} }
} } Best Regards,
} }
} } Stan Schwartz
} } Manager, BioSciences Dept.
} } Nikon Inc Instrument Division
} } 1300 Walt Whitman Rd.
} } Melville, NY 11747
} } 631-547-8500
} } 631-547-4033 Fax
} } Schwartz-at-nikonincmail.com
} } www.nikonusa.com
}
} As a long term past user of Nikon equipment, it is a tale of sorrow.
} Most recently, the digital E1 and E2 are dismal failures. Consumers
} are sending back 990's in droves (see rec.photo.marketplace.digital).
} I really think that Nikon blew it in regards to digicams. How Nikon can
} claim to get a 1.3M pixel imager to produce realistic 12M pixel images
} is rather absurd....if not offensive. The most recent D1 is a joke. Albeit,
} an expensive one.
}
} Nikon blew it in the scanner arena and made huge blunders in high end
} pro digicams (E1 & E2). Total junk from my personal experiences. I am
} not at all prone to spend a dime on any new Nikon digital things. In fact,
} I have
} dumped my Nikon so-called pro lenses and bodies for Contax. But this
} is another story.
}
} My advice is to be very wary....be very wary of Nikon. I do not use Nikon
} cameras anymore and I do not use Nikon microscopes anymore.
} But it is your money and your decision. As they say, "Caveat emptor."
}
} gg
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Modern surfers use PC boards. You can too at
} http://photoweb.net
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Jun 04 12:19:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 4 Jun 2000 12:26:35 -0500
Subject: Administrivia: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
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Colleagues.....

Stop this "tangental thread" on Resolving Power now, before it
gets out of hand.
User/Vendor problems should be handled in private not on the list.


Nestor
Your Friendly Neighborhood SysOp.




From daemon Sun Jun 04 21:46:07 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 4 Jun 2000 16:29:41 -0700
Subject: Project MICRO

Contents Retrieved from Microscopy Listserver Archives
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It's been some time since I posted news about Project MICRO, MSA's middle
school educational outreach program. I'm happy to report that Nestor,
wearing his Webmaster hat (yes, he owns more than one), has just posted a
substantial revision of the MICRO website (URL below). You'll find new
information on several pages and a LOT of new entries in the bibliography.
Don't miss the "Cyclops" videos and the new CD-ROMs; the website hotlinks
are much expanded also.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Jun 04 22:06:23 2000



From: John E. Toth :      jetoth-at-mail.olypen.com
Date: Sun, 4 Jun 2000 21:53:51 -0500
Subject: Microsope design

Contents Retrieved from Microscopy Listserver Archives
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Sirs,

I am looking for books on the subject of optical microscope objective
lens design.
Any suggestions would be appreciated.

John Toth

jetoth-at-olypen.com
or
sciret-at-olypen.com




From daemon Sun Jun 04 22:06:23 2000



From: Toby Knight :      tknight-at-waite.adelaide.edu.au
Date: Mon, 5 Jun 2000 12:29:02 +0930 (CST)
Subject: schiffs staining epoxy sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to stain Procure-Araldite embedded material using the periodic-acid
schiff procedure. One method I have is to hydrolyze in 1N HCl at
60 degrees C for 10 mins, water wash, stain in schiffs, metabisulphate
rinses, counterstain, water wash (O'Brien and McCully, 1981). Does anyone
use a method routinely which varies from this??

thanks in advance, Toby Knight.


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
The University of Adelaide
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064

Tel: +61 8 8303 7224 or 8303 6668
HVO: +61 8 8303 7242
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------





From daemon Sun Jun 04 22:46:41 2000



From: MGMANDERS-at-aol.com
Date: Sun, 4 Jun 2000 22:41:14 -0500
Subject: NEED HELP WITH EDS ON A JEOL 100S

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A fellow colleague has bought a JEOL 100s and wants and needs EDS X-ray
Analysis. He's mounted his horizintal EDS detector , but can not get sample
X-rays from the speciman. Contacting JEOL he found the problem to be a tilt
problem. The 10 degree tilt from the normal SEG only tilts 10 degrees and a
30 to 60 degree tilt is necessary. At one time I was told that special
sample holders and or double gap pole pieces were availabe. He wishs to find
any one with a 100s for parts needed or information which would allow X-ray
analysis. Please reply to mgmanders-at-aol.com or the list server.

Mike Manders




From daemon Sun Jun 04 23:59:57 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 4 Jun 2000 23:46:40 -0500
Subject: Desktop incubator

Contents Retrieved from Microscopy Listserver Archives
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I reacall some one looking for a desktop incubator. I came
a across one on ebay.
http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=344913999
It is a little pricy by ebay standards. You can find a bunch of incubators
by going to www.ebay.com and search for incubators you can do the
same a http://www.labx.com

I don't have any connection with anyone involved in this.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Mon Jun 05 08:21:42 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Sat, 03 Jun 2000 16:35:28 +1000
Subject: Re: schiffs staining epoxy sections

Contents Retrieved from Microscopy Listserver Archives
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Toby,
I used this technique years ago when I worked for Terry O'Brien at
Monash Uni. Depending on the type of tissue you may want to do an
aldehyde blockade before you begin the staining procedure. This
technique is also in O'Brien and McCully. The blockade removes any
aldehyde grouping that will react with the Schiffs reagent. You then
generate and stain aldehyde groups during the procedure.
Regards
JVN

Toby Knight wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I want to stain Procure-Araldite embedded material using the periodic-acid
} schiff procedure. One method I have is to hydrolyze in 1N HCl at
} 60 degrees C for 10 mins, water wash, stain in schiffs, metabisulphate
} rinses, counterstain, water wash (O'Brien and McCully, 1981). Does anyone
} use a method routinely which varies from this??
}
} thanks in advance, Toby Knight.
}
} --------------------------------------------------------
} Toby Knight
} PhD student
}
} Department of Horticulture, Viticulture and Oenology
} The University of Adelaide
} Plant Research Centre
} Waite Campus, PMB 1
} Glen Osmond, SA 5064
}
} Tel: +61 8 8303 7224 or 8303 6668
} HVO: +61 8 8303 7242
} Fax: +61 8 8303 7116
} Email: tknight-at-waite.adelaide.edu.au
} --------------------------------------------------------

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Mon Jun 05 08:21:57 2000



From: Michelle.Taurino-at-aventis.com
Date: Mon, 5 Jun 2000 06:54:20 -0500
Subject: schiffs staining epoxy sections

Contents Retrieved from Microscopy Listserver Archives
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Toby-
I've worked with the PAS stain with methyl methacrylate embedded tissue cut
at 2 microns.
I used a kit from Polyscientific (NY, USA) which supplied me with all of the
necessary reagents (minus the ethanol and xylene). The protocol that was
supplied with the kit is for paraffin embedded material but I made
modifications to the protocol to accommodate methyl methacrylate.
If you are interested in the modified protocol, please contact me.
I should also mention that I automated this stain to save time and to
maximize quality.

Good luck!
Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: Toby Knight [mailto:tknight-at-waite.adelaide.edu.au]
Sent: Sunday, June 04, 2000 10:59 PM
To: Histonet; Microscopy list


I want to stain Procure-Araldite embedded material using the periodic-acid
schiff procedure. One method I have is to hydrolyze in 1N HCl at
60 degrees C for 10 mins, water wash, stain in schiffs, metabisulphate
rinses, counterstain, water wash (O'Brien and McCully, 1981). Does anyone
use a method routinely which varies from this??

thanks in advance, Toby Knight.


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
The University of Adelaide
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064

Tel: +61 8 8303 7224 or 8303 6668
HVO: +61 8 8303 7242
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------




From daemon Mon Jun 05 08:21:58 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Mon, 05 Jun 2000 14:01:28 +0200
Subject: BSE-Thanks

Contents Retrieved from Microscopy Listserver Archives
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Just like to thank all those who responded to my squeal for help on BS
detector resolution. Would like to add that the South African microscopy
community is looking into establishing QA procedures and some of the
suggestions maybe very helpful in this regard.
Thanks,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Mon Jun 05 08:42:06 2000



From: De Pauw Bart :      bart.depauw-at-rug.ac.be
Date: Mon, 05 Jun 2000 15:21:00 +0200
Subject: SEM - Mycoplasms

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Hello Listers,

I've been trying to look at Mycoplasms with SEM. Untill now, the results
are not what we hoped for. We've used polycarbonate filters to prepare the
Mycoplasms. Another way was preparing the Mycoplasms on agar. We have done
this already for enterococcus and we know that this technique works well.
But with the Mycoplasms we see almost nothing. It's like the Mycoplasms are
IN the Agar.
Has anyone have experience with MYCOPLASMS and SEM ???


De Pauw Bart
Ghent University
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90



From daemon Mon Jun 05 10:02:23 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 5 Jun 2000 16:02:06 +0000
Subject: resolution of Technical Pan

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Toby
The procedure you describe is not in fact Periodic Acid-Schiff but
the Feulgen reaction, for visualization of DNA in nuclei and
mitochondria.

The PAS reaction uses periodic acid or sodium meta-periodate
(typically 1% solution, ~10min) to oxidise the vicinal diols of some
polysaccharides which are then stained with pararosaniline Schiff's
reagent. It is not usually necessary to use metabisulphite in the
wash water. The detailed procedure is also described in O'Brien &
McCully 1981.

PAS (and Feulgen) may be viewed in a brightfield microscope or by
fluorescence microscopy with a "rhodamine" filter set. An
alternative fluorescent dye which works well in a
fluorescence pseudo Schiff procedure is Lucifer Yellow CH, which
needs blue excitation (FITC filter set).

Chris


Date sent: Mon, 5 Jun 2000 12:29:02 +0930 (CST)
} From: Toby Knight {tknight-at-waite.adelaide.edu.au}
Send reply to: Toby Knight {tknight-at-waite.adelaide.edu.au}


I just came across the following pearl in a 1982 Kodak publication,
number PDS 61 "Selecting film from Kodak for photomicrography".

"The (Technical Pan) 2415 film has very low granularity and is
capable of very great enlargement. In some cases the resolving
power may be beyond that of the microscope image"

How do they know!
Answers on a postcard please ....

Chris
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Mon Jun 05 10:12:29 2000



From: Charlesworth, Jon :      charlesworth.jon-at-mayo.edu
Date: Mon, 5 Jun 2000 10:06:40 -0500
Subject: Technician Position

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Electron Microscopy Core Facility at Mayo Clinic

The Electron Microscopy Core Facility at Mayo Clinic in
Rochester, MN has an opening for a Biomedical EM technologist to support
both clinical and research projects. The laboratory offers expertise to
collaborative projects that involve transmission and scanning electron
microscopy. The laboratory is well equipped and has a history of excellent
productivity and adequate funding.

The successful candidate for this position will possess at
least a bachelor's degree in Biology with experience in histology and/or
electron microscopy. Additional courses or experience in Immunology, Cell
Biology, and Digital Imaging is desirable. Operating knowledge of
transmission and scanning electron microscopes is preferred. The applicant
must have excellent communicative skills and the ability to work well with a
variety of personalities.

The EM Technologist interacts with all laboratory users in
order to accomplish specific research and clinical goals with respect to
electron microscopy procedures. Duties include: All aspects of specimen
preparation for a variety of biomedical samples for TEM and SEM, operation
of TEM and SEM, darkroom developing and printing, digital image capture, and
reporting. The technologist will also perform advanced research procedures
including immunoelectron microscopy, x-ray microanalysis, and microwave
processing.

Mayo offers a competitive salary and benefits package. If
interested, please submit a cover letter and resume referencing job posting
#00-0002365 to:

Jill Kelly
Mayo Medical Center
Human Resources-OE 1
Rochester, MN 55905
Fax: 507-284-1445
Email: kelly.jill-at-mayo.edu



Jon Charlesworth
Coordinator
Electron Microscopy Core Facility
1426 Gugg
X4-3148



From daemon Mon Jun 05 10:32:17 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 5 Jun 2000 09:10:09 -0600
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim wrote:

'I have two replies and Mike my well have four replies to these.'

I'll try. But I agree with you that we should let this thread die. jim,
it seems you're a bit angy at me. If I unintentionally stepped on your
toe I apologize. I did not expect that we would agree 100%, as you are
selling film and I am involved in digital systems. I just hope that some
other readers found some of this useful. I don't consider myself a
"digital fanatic". This film vs. digital issue has many more facets,
some of which we did not even touch, and which can be just as
entertaining.

Jim wrote:

'Mike reinforced and strengthened that argument, finishing with the note
that he is glad for film when looking at prints by Ansel Adams.'

Just for the record: I usually do not take a magnifying glass to photos.
I mentioned Adams because I like his pictures. If he had taken them with
a digital camera I would have liked them just as much. I also like some
modern art paintings. That does not mean we should start drawing what we
see in the microscopes rather than taking pictures ;-)

Jim wrote:

'Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM'

Well, you said 'When great enlargements are required film is superior'.
Perhaps I misunderstood. I thought, the term great enlargements referred
to the microscope and meant 'small details', which you can take easier
with a digital camera (no time delay between seeing something and taking
an image, no mechanical vibration due to film movement, etc.). I have
said many times before that film may be better if high resolution AND
large field of view is required.

Jim wrote:

'in any case: Show Sergey!'

I'm in back-channel correspondence with him.



Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Sunday, June 04, 2000 2:34 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


I have two replies and Mike my well have four replies to these.
1. There has been a parallel discussion concerning resolution of
film versus
digital images. Clearly in raw power digital cannot compete since film
has
multi gigabyte capacity. I added to that thread that what matters is:
does
digital have enough power and that frequently it would. Mike reinforced
and
strengthened that argument, finishing with the note that he is glad for
film
when looking at prints by Ansel Adams.
(Ansel Adams until about 30 years ago carted for decades large format
cameras
through US National Parks, especially Yosemite, producing fantastic
landscape
photographs and books)
Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet
film,
probably rated at 400 ISO. The line resolution of such prints much
exceeds our
eyes' resolution, but still results in superior gradation and detail.
TEM film,
even when much enlarged has such details too.
Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM,
and
because of the limited enlargability of light microscopy (concrete
ceiling due
to wavelengths) it's reasonable, but minimal for light microscopy. TEM
can do
and deserves better.

Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM, as one of films major advantages, since greater depths
of field
at moderate powers makes high powers through photo enlarging a desirable

technique. The small additional magnification yielded by placing a
digital
camera lower in the column does not compensate. So greater
"enlargability" of
digitals would be desirable, but is limited by pixel size.

2 The thread was initiated by Sergey. He had problems visualising
certain
specimens in dark field. The use of his "beaut" digital TEM camera made
things
worse. I pointed out that the shorter exposure reduced the number of
electrons
forming the image, hence more noise. I believe that a good part of
Mike's case
will be settled in his favour when we hear "Eureka" from Sergey's lab. I
don't
doubt that much more can be done with digital now and that further
improvements
are on the way. Mike's "solution" may well be possible, but I don't
believe its
a snap; in any case: Show Sergey!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
wrote:
}
} Well, let's see:
}
} Jim wrote:
}
} Mike Bode would use multiple digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
}
} No, I did not talk about further enlargements. All I wanted to say is,
} that a more noise-free image can be achieved by adding multiple
images,
} and that this also to some extent helps with drift of the sample
during
} acquisition.
}
} Jim wrote:
}
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure?
}
} How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} information is about 2.5 MB (uncompressed). We acquire about 10 of
those
} per second and transfer them across the PC bus to the display. Putting
} them on them into Memory might add a few tenth of a second. Writing to
} HD can be done after all images are acquired.
}
} Jim wrote:
}
} What would be the total time from focusing to the last exposure?
} What about Z-drift in the interim requiring objective changes
}
} Why would we have to worry about that, if we don't have to worry about
} that when taking the image on film? In fact, we could take care of
this
} by looking at the image between exposures and correct for z-drift.
} However, as you said, that would add to the overall time and exposure.
I
} was comparing a normal dark field image taken on film at 8 seconds
with
} acquiring the same image on a "too sensitive" CCD camera by adding up
10
} consecutive .8 second images. Why would the sample drift (in x, y or
z)
} substantially more in 8+delta seconds than in 8?
}
} Jim wrote:
}
} what about the total cost of this additional get-up
}
} That of course depends on the microscope and there is no general
answer.
} For example on a LEO 912 I believe the blanker is standard. The
} additional cost to use an acquisition scheme like this with our
software
} is $0 plus perhaps a bit of time to write a small macro. On other
} microscopes one might have to add a beam blanker and perhaps a control
} mechanism for the beam blanker. But I would guess, that this cost is
not
} very high. All modern microscopes are computer controller anyway, so
it
} is most likely just a control command that needs to be sent to the
} microscope over a serial port if the beam blanker is installed. Piece
of
} cake.
}
} Jim wrote:
}
} The mind boggles at a through focus series.
}
} You're right here. But I don't think we were talking about
through-focus
} series. Incidentally, we do through-focus series on light microscopes
} and reconstruction routinely. Takes a few images at different focus
(or
} for a light microscope: stage) settings. The rest is done off-line.
} Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} agree that TEM is different here and much more complicated due to the
} complicated Contrast Transfer Function. However, this could in
principle
} be sorted out.
}
} Jim wrote:
}
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
}
} I also agree with you on that one. But using the additional computer
} possibilities of digital imaging might take you further than expected.
}
} Michael
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Friday, June 02, 2000 5:15 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'jim-at-proscitech.com.au'
} Subject: RE: Film vs Digital
}
}
} The world is full of possible solutions, but are they practical.?
} To produce high-resolution, dark-field or any others TEM images that
} require
} more electrons to form a clear image, Mike Bode would use multiple
} digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
} Beam blanking would largely save the specimen from beam damage and
drift
} could
} be compensated for by matching up the digitals. Great.
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure? What would be the total time from focusing to the last
} exposure?
} What about Z-drift in the interim requiring objective changes and what
} about
} the total cost of this additional get-up. The mind boggles at a
through
} focus
} series.
} When pushing the limits a piece of film seems more effective, cheaper
} and fa
} ster.
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} wrote:
} }
} } No, it's not really a problem. It's been done with low density
} } microscopy all the time. Granted, there are some technical aspects
to
} be
} } overcome, but (and I can only speak for ourselves) we have done that
} on
} } a number of microscopes. You are of course correct, that 10 images
at
} } 0.8 seconds take longer than 8 seconds as the image has to be
} } transferred, etc. BUT: that's what beam blankers are for. It is
pretty
} } straightforward to take an image at 0.8 seconds, then blank the beam
} } very quickly before taking the next image. That way you get pretty
} close
} } to the 8 sec total exposure. If there is no beam blanker on the
} } microscope, in most cases it can be added.
} }
} } I am not sure what you mean by "too sensitive". The cameras are
} usually
} } constructed so that 1 electron from the beam creates between a few
} tenth
} } to a few counts (these are all statistical data, of course). The
well
} } width divided by this sensitivity then determines, how many primary
} } electrons are needed to fully expose one pixel. For example, if the
} well
} } width is 50,000 electrons and the sensitivity is 1 count/electron,
one
} } needs 50,000 primary electrons to fill the well. This translates
into
} } roughly a 0.4% statistical error.
} }
} } } From a practical standpoint: You can take images with most cameras
} when
} } the exposure meter on the microscope reads a couple of seconds
without
} } overexposing the camera. On the other hand, you can reduce the
} intensity
} } of the beam until you see single electron events.
} }
} } The one area where CCD cameras may be too sensitive is diffraction.
} The
} } normally huge intensity in the transmitted beam often leads to
} } saturation. In CCDs this can lead to blooming (the intensity spills
} over
} } into neighboring pixels). This can be taken care of with special
chips
} } that have anti-blooming features, but this usually has some other
} } drawbacks. Again, this can also be overcome somewhat with multiple
} } exposures. Film behaves more civilized here, as it simply stops
} } responding to the electrons, but this makes film more or less
useless
} } for quantitative measurements of diffraction patterns. I have done
} } diffraction with CCDs many times and though it does require some
} } tweaking, one can get very good results from them.
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Wednesday, May 31, 2000 9:37 PM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: RE: Film vs Digital
} }
} }
} }
}
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
-----------------------------------------------------------------------.
} }
} }
} } Yes, it could be done "in theory". Somebody would need to figure out
} the
} }
} } software and perhaps modify the hardware. Then we would find that
the
} } total
} } exposure of the specimen to the electron beam maybe a muliple of the
} } film's
} } exposure. Afterall, an 8 sec film exposure would not amount in
digital
} } to
} } 10x0.8, but we would require considerable time in between exposures.
} } Since the
} } problems in the discussed circumstances are specimen movement and
beam
} } damage,
} } it seems that taking multiple exposures is a poor option.
} }
} } Digital cameras are for some situation too sensitive to electron
} } exposure.
} } Cutting back on electrons is no option since its the electrons that
} form
} } the
} } image in the first instance.
} } Much easier in light microscopy . . . insert a neutral density
} filter.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }



From daemon Tue Jun 06 23:33:12 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 5 Jun 2000 10:55:39 -0500
Subject: Re: Administrivia: Resolving power: - Clarification from Nestor

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Jonathon
Low kV is preferred for some biological applications chiefly
because biologists experience difficulty getting enough contrast out
of their specimens. As Dr Alves de Matos rightly says, low kV is
more damaging to specimens than high kV, because the
penetrating power of low-kV electrons is lower and therefore more
beam energy is lost to the specimen. (We think of this exactly the
other way round for SEM, but that's a story for another day!).

The main reason for the apparent shift in biologist's attitudes is that
many are now less concerned with cell and tissue-level
ultrastructure and increasingly interested in imaging
macromolecules. 200kV or 300kV machines are preferred for cryo-
microscopy of macromolecules in vitrified ice because the
resolution is a lot better than at 80 or 120kV, and also because
200kV causes less specimen damage.

However, the cost of resolution either from higher kVs or the
shorter working distance objective lenses favoured by materials
scientists is poorer image contrast. Philips therefore supply
"Biotwin" versions of some of their TEM range which use a long
focal length objective which trading a little resolution for a little
more contrast. This optical configuration is coincidentally very good
for thickish specimens such as ultrathin biological sections where
the ultimate resolution of the machine is pretty much academic
anyway.

Images of thick (#100nm) specimens such as FIB sections of
microelectronic devices seem crisper and more contrasty in our
CM120 Biotwin than in a 200KV machine.
Chris

} From: "A.P.Alves de Matos" {apmatos-at-ip.pt}
To: {Microscopy-at-sparc5.microscopy.com}


Colleagues....

Some of you have misinterpreted my earlier message.
Please feel free to carry on the discussion on film/digitization etc..

I only asked that the "tangental thread" centered about
complaints on a specific product be taken off-line before
any fire and brimstone starts up between a manufacturer
and clients. This is not the forum for that type of feedback.

The previous dicussion should certainly continue as long
as necessary.

Nestor
Your Friendly Neighborhood SysOp



}
} Nestor,
}
} It would be very unfortunate to squelch this important and central debate
} concerning not only the corresponding resolution comparisons but also
} differances in performance due to noise and greyscale depth. New methods of
} enhansing resolution need to be critiqued as well.
}
} Dave Barnard
}
} Wadsworth Center HVEM
} NYS Dept. Health
} Albany NY





From daemon Tue Jun 06 23:33:24 2000



From: Sha Zhu :      szhu-at-engin.umich.edu
Date: Mon, 5 Jun 2000 16:00:52 -0400 (EDT)
Subject: TEM: looking for T-tool

Contents Retrieved from Microscopy Listserver Archives
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We are looking for the "T-tool" - a kind of fixture for TEM sample
polishing. It is from T&T group in USA. We need to know the email
address or telephone number of T&T group.

Any information about the T&T group or the T-tool will be highly
appreciated!

Thanks a lot!

szhu




From daemon Tue Jun 06 23:33:25 2000



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Mon, 5 Jun 2000 13:30:28 -0700 (PDT)
Subject: Wanted: Used JEOL 2010 TEM

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We are looking to purchase a used JEOL 2010 TEM (LaB6, not FE) in good
working condition. I would appreciate replies from anyone who knows if a
JEOL 2010 is available or will become available in the next 6 months or
so. A STEM unit would increase our interest, but isn't strictly
necessary.

Also, we will be looking for a buyer for our JEOL 1200EX STEM which has
been under service contract from day 1. It has been a highly reliable
microscope and has given years of satisfactory performance.

Dave Joswiak
Research Scientist
Dept. of Astronomy, 351580
University of Washington
Seattle, WA 98195
(206)543-7702
joswiak-at-astro.washington.edu





From daemon Tue Jun 06 23:33:26 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 5 Jun 2000 16:14:59 -0500
Subject: SEM: softening of beetle elytra

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I am forwarding this request on behalf of Mr. Edoardo Nolfo. If
anyone could be of assistance please send the information to him
directly or (if you prefer) I will forward it to him.

Thank you.

J.B.


} From: Edoardo Nolfo {edoardo.nolfo-at-christ-church.oxford.ac.uk}

} I am a student at the University of Oxford and am writing to ask for some
} advice. I am currently working on multilayer reflectors in beetles; I plan to
} use S.E.M. and T.E.M. to elucidate the fine structure of these reflectors.
} Apparently the elytra of the beetles must be sliced very thinly for S.E.M.
} analysis. My problem lies in the fact that the elytra are extremely
} hard, which
} means they cannot be sliced very thinly. Do you have any advice to
} offer on how
} to soften the elytra for this purpose? Perhaps there is a chemical
} that is used
} to make samples softer, or a standard technique used to soften very hard
} samples. I would be extremely grateful to you if you could help me! Please do
} not hesitate to contact me if you require any more information.
}
} Let me thank you in advance for your help; I look forward to hearing from you
} soon.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Tue Jun 06 23:33:33 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Sun, 04 Jun 2000 08:54:05 +1000
Subject: Re: resolution of Technical Pan

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Chris,
This is because Kodak use EMs to measure the grain size of their silver
halide grains. Tech Pan has a very small grain size (slow film, low
ISO/ASA number) and is therefore capable of greater enlargement than
film with a larger grains. The larger the grain the faster the film
(higher ISO/ASA number).
Regards
JVN
JVN

Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I just came across the following pearl in a 1982 Kodak publication,
} number PDS 61 "Selecting film from Kodak for photomicrography".
}
} "The (Technical Pan) 2415 film has very low granularity and is
} capable of very great enlargement. In some cases the resolving
} power may be beyond that of the microscope image"
}
} How do they know!
} Answers on a postcard please ....
}
} Chris
} =====================================================================
} DR CHRIS JEFFREE
} BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} UNIVERSITY OF EDINBURGH
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 131 650 5345
} FAX. #44 131 650 6563
} Mobile 0410 585 401
} email c.jeffree-at-ed.ac.uk
} SEM / TEM bookings sem-at-ed.ac.uk
} =====================================================================

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Tue Jun 06 23:33:41 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 5 Jun 2000 21:32:46 -0400
Subject: TEM: looking for T-tool

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Zhu:

The "T" tool is a variation of the well known Tripod Polisherš. We are the
manufacturers of the Tripod Polisherš and also manufacture the more compact
BiPod Polisher. The BiPod polisher offers the smaller size as found on the
T-tool while still incorporating our many years of experience in SEM and
TEM cross section polishing as well as our expertise in precision
machining. I know this doesn't help much in locating the T-tool, but it
does offer you the information you need to acquire a tool that will
effectively meet your requirements. If you would like additional
information on these tools, please feel free to contact me.

Best regards-

David
Writing at 6:24:03 PM on 06/05/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.



Message text written by Sha Zhu
} -----------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




We are looking for the "T-tool" - a kind of fixture for TEM sample
polishing. It is from T&T group in USA. We need to know the email
address or telephone number of T&T group.

Any information about the T&T group or the T-tool will be highly
appreciated!

Thanks a lot!


szhu

{



From daemon Tue Jun 06 23:33:46 2000



From: Khanh Tran :      khanh-at-deakin.edu.au
Date: Tue, 06 Jun 2000 14:01:40 +1000
Subject: subscribe

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Hello ,

please subscribe
Khanh Tran
Deakin University
662 Blackburn Road
CLAYTON, VIC. 3168
AUSTRALIA



From daemon Tue Jun 06 23:33:52 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 5 Jun 2000 09:10:09 -0600
Subject: RE: Film vs Digital

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Jim wrote:

'I have two replies and Mike my well have four replies to these.'

I'll try. But I agree with you that we should let this thread die. jim,
it seems you're a bit angy at me. If I unintentionally stepped on your
toe I apologize. I did not expect that we would agree 100%, as you are
selling film and I am involved in digital systems. I just hope that some
other readers found some of this useful. I don't consider myself a
"digital fanatic". This film vs. digital issue has many more facets,
some of which we did not even touch, and which can be just as
entertaining.

Jim wrote:

'Mike reinforced and strengthened that argument, finishing with the note
that he is glad for film when looking at prints by Ansel Adams.'

Just for the record: I usually do not take a magnifying glass to photos.
I mentioned Adams because I like his pictures. If he had taken them with
a digital camera I would have liked them just as much. I also like some
modern art paintings. That does not mean we should start drawing what we
see in the microscopes rather than taking pictures ;-)

Jim wrote:

'Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM'

Well, you said 'When great enlargements are required film is superior'.
Perhaps I misunderstood. I thought, the term great enlargements referred
to the microscope and meant 'small details', which you can take easier
with a digital camera (no time delay between seeing something and taking
an image, no mechanical vibration due to film movement, etc.). I have
said many times before that film may be better if high resolution AND
large field of view is required.

Jim wrote:

'in any case: Show Sergey!'

I'm in back-channel correspondence with him.



Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Sunday, June 04, 2000 2:34 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


I have two replies and Mike my well have four replies to these.
1. There has been a parallel discussion concerning resolution of
film versus
digital images. Clearly in raw power digital cannot compete since film
has
multi gigabyte capacity. I added to that thread that what matters is:
does
digital have enough power and that frequently it would. Mike reinforced
and
strengthened that argument, finishing with the note that he is glad for
film
when looking at prints by Ansel Adams.
(Ansel Adams until about 30 years ago carted for decades large format
cameras
through US National Parks, especially Yosemite, producing fantastic
landscape
photographs and books)
Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet
film,
probably rated at 400 ISO. The line resolution of such prints much
exceeds our
eyes' resolution, but still results in superior gradation and detail.
TEM film,
even when much enlarged has such details too.
Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM,
and
because of the limited enlargability of light microscopy (concrete
ceiling due
to wavelengths) it's reasonable, but minimal for light microscopy. TEM
can do
and deserves better.

Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM, as one of films major advantages, since greater depths
of field
at moderate powers makes high powers through photo enlarging a desirable

technique. The small additional magnification yielded by placing a
digital
camera lower in the column does not compensate. So greater
"enlargability" of
digitals would be desirable, but is limited by pixel size.

2 The thread was initiated by Sergey. He had problems visualising
certain
specimens in dark field. The use of his "beaut" digital TEM camera made
things
worse. I pointed out that the shorter exposure reduced the number of
electrons
forming the image, hence more noise. I believe that a good part of
Mike's case
will be settled in his favour when we hear "Eureka" from Sergey's lab. I
don't
doubt that much more can be done with digital now and that further
improvements
are on the way. Mike's "solution" may well be possible, but I don't
believe its
a snap; in any case: Show Sergey!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
wrote:
}
} Well, let's see:
}
} Jim wrote:
}
} Mike Bode would use multiple digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
}
} No, I did not talk about further enlargements. All I wanted to say is,
} that a more noise-free image can be achieved by adding multiple
images,
} and that this also to some extent helps with drift of the sample
during
} acquisition.
}
} Jim wrote:
}
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure?
}
} How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} information is about 2.5 MB (uncompressed). We acquire about 10 of
those
} per second and transfer them across the PC bus to the display. Putting
} them on them into Memory might add a few tenth of a second. Writing to
} HD can be done after all images are acquired.
}
} Jim wrote:
}
} What would be the total time from focusing to the last exposure?
} What about Z-drift in the interim requiring objective changes
}
} Why would we have to worry about that, if we don't have to worry about
} that when taking the image on film? In fact, we could take care of
this
} by looking at the image between exposures and correct for z-drift.
} However, as you said, that would add to the overall time and exposure.
I
} was comparing a normal dark field image taken on film at 8 seconds
with
} acquiring the same image on a "too sensitive" CCD camera by adding up
10
} consecutive .8 second images. Why would the sample drift (in x, y or
z)
} substantially more in 8+delta seconds than in 8?
}
} Jim wrote:
}
} what about the total cost of this additional get-up
}
} That of course depends on the microscope and there is no general
answer.
} For example on a LEO 912 I believe the blanker is standard. The
} additional cost to use an acquisition scheme like this with our
software
} is $0 plus perhaps a bit of time to write a small macro. On other
} microscopes one might have to add a beam blanker and perhaps a control
} mechanism for the beam blanker. But I would guess, that this cost is
not
} very high. All modern microscopes are computer controller anyway, so
it
} is most likely just a control command that needs to be sent to the
} microscope over a serial port if the beam blanker is installed. Piece
of
} cake.
}
} Jim wrote:
}
} The mind boggles at a through focus series.
}
} You're right here. But I don't think we were talking about
through-focus
} series. Incidentally, we do through-focus series on light microscopes
} and reconstruction routinely. Takes a few images at different focus
(or
} for a light microscope: stage) settings. The rest is done off-line.
} Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} agree that TEM is different here and much more complicated due to the
} complicated Contrast Transfer Function. However, this could in
principle
} be sorted out.
}
} Jim wrote:
}
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
}
} I also agree with you on that one. But using the additional computer
} possibilities of digital imaging might take you further than expected.
}
} Michael
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Friday, June 02, 2000 5:15 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'jim-at-proscitech.com.au'
} Subject: RE: Film vs Digital
}
}
} The world is full of possible solutions, but are they practical.?
} To produce high-resolution, dark-field or any others TEM images that
} require
} more electrons to form a clear image, Mike Bode would use multiple
} digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
} Beam blanking would largely save the specimen from beam damage and
drift
} could
} be compensated for by matching up the digitals. Great.
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure? What would be the total time from focusing to the last
} exposure?
} What about Z-drift in the interim requiring objective changes and what
} about
} the total cost of this additional get-up. The mind boggles at a
through
} focus
} series.
} When pushing the limits a piece of film seems more effective, cheaper
} and fa
} ster.
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} wrote:
} }
} } No, it's not really a problem. It's been done with low density
} } microscopy all the time. Granted, there are some technical aspects
to
} be
} } overcome, but (and I can only speak for ourselves) we have done that
} on
} } a number of microscopes. You are of course correct, that 10 images
at
} } 0.8 seconds take longer than 8 seconds as the image has to be
} } transferred, etc. BUT: that's what beam blankers are for. It is
pretty
} } straightforward to take an image at 0.8 seconds, then blank the beam
} } very quickly before taking the next image. That way you get pretty
} close
} } to the 8 sec total exposure. If there is no beam blanker on the
} } microscope, in most cases it can be added.
} }
} } I am not sure what you mean by "too sensitive". The cameras are
} usually
} } constructed so that 1 electron from the beam creates between a few
} tenth
} } to a few counts (these are all statistical data, of course). The
well
} } width divided by this sensitivity then determines, how many primary
} } electrons are needed to fully expose one pixel. For example, if the
} well
} } width is 50,000 electrons and the sensitivity is 1 count/electron,
one
} } needs 50,000 primary electrons to fill the well. This translates
into
} } roughly a 0.4% statistical error.
} }
} } } From a practical standpoint: You can take images with most cameras
} when
} } the exposure meter on the microscope reads a couple of seconds
without
} } overexposing the camera. On the other hand, you can reduce the
} intensity
} } of the beam until you see single electron events.
} }
} } The one area where CCD cameras may be too sensitive is diffraction.
} The
} } normally huge intensity in the transmitted beam often leads to
} } saturation. In CCDs this can lead to blooming (the intensity spills
} over
} } into neighboring pixels). This can be taken care of with special
chips
} } that have anti-blooming features, but this usually has some other
} } drawbacks. Again, this can also be overcome somewhat with multiple
} } exposures. Film behaves more civilized here, as it simply stops
} } responding to the electrons, but this makes film more or less
useless
} } for quantitative measurements of diffraction patterns. I have done
} } diffraction with CCDs many times and though it does require some
} } tweaking, one can get very good results from them.
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Wednesday, May 31, 2000 9:37 PM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: RE: Film vs Digital
} }
} }
} }
}
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
-----------------------------------------------------------------------.
} }
} }
} } Yes, it could be done "in theory". Somebody would need to figure out
} the
} }
} } software and perhaps modify the hardware. Then we would find that
the
} } total
} } exposure of the specimen to the electron beam maybe a muliple of the
} } film's
} } exposure. Afterall, an 8 sec film exposure would not amount in
digital
} } to
} } 10x0.8, but we would require considerable time in between exposures.
} } Since the
} } problems in the discussed circumstances are specimen movement and
beam
} } damage,
} } it seems that taking multiple exposures is a poor option.
} }
} } Digital cameras are for some situation too sensitive to electron
} } exposure.
} } Cutting back on electrons is no option since its the electrons that
} form
} } the
} } image in the first instance.
} } Much easier in light microscopy . . . insert a neutral density
} filter.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }




From daemon Tue Jun 06 23:33:52 2000



From: Didier Le Thiec :      le_thiec-at-nancy.inra.fr
Date: Tue, 6 Jun 2000 09:09:33 -0500
Subject: leaves

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Jonathon
Low kV is preferred for some biological applications chiefly
because biologists experience difficulty getting enough contrast out
of their specimens. As Dr Alves de Matos rightly says, low kV is
more damaging to specimens than high kV, because the
penetrating power of low-kV electrons is lower and therefore more
beam energy is lost to the specimen. (We think of this exactly the
other way round for SEM, but that's a story for another day!).

The main reason for the apparent shift in biologist's attitudes is that
many are now less concerned with cell and tissue-level
ultrastructure and increasingly interested in imaging
macromolecules. 200kV or 300kV machines are preferred for cryo-
microscopy of macromolecules in vitrified ice because the
resolution is a lot better than at 80 or 120kV, and also because
200kV causes less specimen damage.

However, the cost of resolution either from higher kVs or the
shorter working distance objective lenses favoured by materials
scientists is poorer image contrast. Philips therefore supply
"Biotwin" versions of some of their TEM range which use a long
focal length objective which trading a little resolution for a little
more contrast. This optical configuration is coincidentally very good
for thickish specimens such as ultrathin biological sections where
the ultimate resolution of the machine is pretty much academic
anyway.

Images of thick (#100nm) specimens such as FIB sections of
microelectronic devices seem crisper and more contrasty in our
CM120 Biotwin than in a 200KV machine.
Chris

} From: "A.P.Alves de Matos" {apmatos-at-ip.pt}
To: {Microscopy-at-sparc5.microscopy.com}


Dear all,

1) I am working on oak trees, and I would like to describe the
ultrastucture of leaves; also I am looking for a protocol (very detailed)
to do it.
2) On oak leaves I would like to localize the superoxyde
dismutase(s) enzymes by immunocytochemistry, also I am looking for a
protocol (very detailed) to do it.

Thanks in advances.
Dr. Didier Le Thiec

--------------------------------------------
Didier Le Thiec
I.N.R.A. Centre de Recherches Forestieres
Unite d'Ecophysiologie Forestiere
Laboratoire de Pollution Atmospherique
54280 Champenoux - France

Tel : 33 (0)3 83 39 40 98
Fax : 33 (0)3 83 39 40 69
E-mail : le_thiec-at-nancy.inra.fr
http://vectra.nancy.inra.fr/pollu/index.htm
-------------------------------------------




From daemon Tue Jun 06 23:33:57 2000



From: Rafal Dunin-Borkowski :      rafal.db-at-materials.oxford.ac.uk
Date: Tue, 6 Jun 2000 09:26:36 +0100 (BST)
Subject: FEG/TEM one-day meeting, Monday 3 July 2000

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Monday 3 July 2000

New Developments in FEG/TEM

A joint one-day meeting organised by RMS and EMAG

Department of Materials, Oxford University

Following on from the very successful meeting held in 1999, this will
review the current state-of-the-art of the UK's various FEG/TEM
installations in operation.

The meeting will begin with a special keynote lecture on

Advanced TEM Instrumentation for Solving Critical Problems in Materials
Science given by Professor Manfred RŸhle Max-Planck Institute fŸr
Metallforschung, Stuttgart

This lecture will include some news on the new German FEG/TEM project.

There are now ten FEG/TEM facilities in the UK (some with more than one
instrument) and the main programme will consist of a series of invited
presentations from each of the sites, reviewing the latest developments in
instrumentation and covering specialised techniques such as holography,
energy-filtered imaging, spectroscopic imaging, nano-scale analysis, etc.

With JIF, JREI and other special equipment funding now becoming available
it is likely that there will be other sophisticated FEG/TEM installations
in place in the near future. With this in view the meeting will also
include a forward look: what will the next generation of instruments be
like?

Speakers:

John Hutchison (University of Oxford)

Key-note Lecture - Professor Manfred RÄhle (Stuttgart)
Per Bullough (University of Sheffield)
Marin van Heel (Imperial College)
John Berriman (MRC, Cambridge)
Tony Cullis (University of Sheffield)
Ian Jones (University of Birmingham)
Rik Brydson (University of Leeds)
Jeremy Sloan (University of Oxford)
David Cherns (University of Bristol)
Stephen McVitie (Glasgow University)
Stephen Lloyd (University of Cambridge)
John Titchmarsh (University of Oxford)
Paul Midgley (University of Cambridge)


The meeting will conclude with the RMS AGM and Presidential Address.

For further details contact:
Dr Paul Midgley (EMAG): pam33-at-cam.ac.uk or
Dr John Hutchison (RMS): john.hutchison-at-materials.ox.ac.uk



From daemon Tue Jun 06 23:34:11 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 6 Jun 2000 11:06:50 -0400
Subject: Re: resolution of Technical Pan

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Greetings,
Just for the record, Tech Pan is interesting in that you can
vary the grain size over an amzingly wide range by choice of
developer and exposure conditions. You can technidol LC (I think) and
get the teeeny weeeeny grains previously mentioned, or use D19 (I
think) and get really high contrast big grains.

Just my few exposed halides,
Tobias



} Chris,
} This is because Kodak use EMs to measure the grain size of their silver
} halide grains. Tech Pan has a very small grain size (slow film, low
} ISO/ASA number) and is therefore capable of greater enlargement than
} film with a larger grains. The larger the grain the faster the film
} (higher ISO/ASA number).
} Regards
} JVN
} JVN
}
} Chris Jeffree wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } I just came across the following pearl in a 1982 Kodak publication,
} } number PDS 61 "Selecting film from Kodak for photomicrography".
} }
} } "The (Technical Pan) 2415 film has very low granularity and is
} } capable of very great enlargement. In some cases the resolving
} } power may be beyond that of the microscope image"
} }
} } How do they know!
} } Answers on a postcard please ....
} }
} } Chris
} } =====================================================================
} } DR CHRIS JEFFREE
} } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} } UNIVERSITY OF EDINBURGH
} } Daniel Rutherford Building
} } King's Buildings, Mayfield Road
} } EDINBURGH, EH9 3JH, Scotland, UK
} } Tel. #44 131 650 5345
} } FAX. #44 131 650 6563
} } Mobile 0410 585 401
} } email c.jeffree-at-ed.ac.uk
} } SEM / TEM bookings sem-at-ed.ac.uk
} } =====================================================================
}
} --
} ****************************************************
} John V Nailon
} Operations Manager
} Centre for Microscopy and Microanalysis
} The University of Queensland
} St. Lucia Queensland 4072
} Phone: +61-7-3365-4214
} Fax: +61-7-3365-4422
} WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
} ****************************************************

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Tue Jun 06 23:34:16 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, May 24, 2000 10:58 AM
Subject: Re: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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MSA Listers:

Two weeks ago I posted a short message about the Ocean Optics spectrometer I
had just received for doing Plasma diagnostics. I tried it and have found it
very useful for examining a plasma cleaning process and optimizing operating
conditions. I purchased a USB 2000 model that plugs into a USB port of a PC
for less than $3000. Ocean Optics, 727-733-2447, www.OceanOptics.com

Ronald Vane
XEI Scientific
NEW WEB SITE! www.SEMCLEAN.com


-----Original Message-----
} From: Ronald Vane {RVaneXEI-at-concentric.net}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com} ; Dr. Gary Gaugler
{gary-at-gaugler.com}



From daemon Tue Jun 06 23:34:18 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 6 Jun 2000 10:17:46 -0700
Subject: SEM: agency wants SEM images?

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My SEM facility has just been approached, and in the interest in
providing what they want for a price based on an average, can I query
the SEM community as to what they charge for commercial release of
their SEM imagery. I'm looking for $/image, but if you charge on any
type of sliding scale, I'd be interested in that too. I believe as
well I shouldn't be undercutting commercial facilities, so I'm
especially interested in these numbers.
Reply to me direct, and I'll respond back to the list with the
average, max and min, and no names.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Jun 06 23:34:19 2000



From: anderron-at-us.ibm.com
Date: Tue, 6 Jun 2000 13:32:30 -0400
Subject: Fluorescence in-situ Hybridization (FISH) Course

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This individual contacted the MSA Business Office with the following
request. Please help her if you can, offline. Don't reply back to me.

Thanks,

..snip

Content-Type: text/plain
Content-Disposition: inline

I work for North Carolina State University and we are looking in to
using Fluorescence in-situ Hybridization (FISH) for identification of
bacteria in biofilm. Do you know of any short courses in FISH here in
the USA? I found some out of the country but would prefer to take a
course in USA.

Thanks for your time

Tracey L. Daly {tldaly-at-unity.ncsu.edu}




Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



From daemon Tue Jun 06 23:34:23 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 6 Jun 2000 11:13:51 -0700
Subject: PGT parts for anyone

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Hi:

I am about to tear the guts out of an old PGT System4 console. I need some
of the electronics, but will toss the rest unless someone would like it.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Jun 06 23:34:46 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 6 Jun 2000 15:27:35 -0400 (EDT)
Subject: UV optics

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I have an associate who is doing cathodoluminescence studies on materials.
He would like to extend his observations into the UV for a limited number of
tests on a a few samples. He is presently limited by the optics of the
microscope and the fiber optics cable connecting the microscope ocular to
the input of the spectrometer. Does anyone have any suggestions for optical
microscopes with UV optics that might be available for short term lease or
loan that he could consider? Objective magnifications of 5X to 10 X and a 5X
or 10X ocular would probably suffice.

Thanks,

Don Marshall


Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Jun 06 23:34:51 2000



From: Jane Fagerland :      jane.a.fagerland-at-abbott.com
Date: Tue, 6 Jun 2000 16:22:14 -0500
Subject: Position Opening - Biological Electron Microscopy

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The Department of Microscopy and Microanalysis at Abbott Laboratories is
recruiting an Electron Microscopist for its Biological Microscopy group.
This group provides ultrastructural pathology support for Nonclinical Drug
Safety studies, as well as for other biological microscopy projects, such as
cell screening, virus identification and counting, and immunolabeling.

Requirements for this position include:
a Masters' degree in a biological field, such as cell biology, anatomy, or
zoology
a thorough understanding of mammalian histology and ultrastructure
excellent technical skills including tissue collection at necropsy, tissue
and cell processing for TEM, sectioning, staining, operation of electron
microscopes and related equipment, darkroom procedures
ability to communicate information in technical reports and in oral
presentations

Highly desirable, but not essential, are:
experience in ultrastructural pathology or toxicologic pathology
working knowledge of SEM specimen preparation and instrumentation
working knowledge of immunocytochemistry, in situ hybridization, and other
labeling methods at light or electron microscopic level
familiarity with Good Laboratory Practices

We are looking for a team player with outstanding interpersonal skills and
the ability to adjust readily to rapidly changing priorities and shifting
deadlines. The ability to communicate clearly, both verbally and in writing,
is essential. Careful attention to detail and accuracy are required.

The Department of Microscopy and Microanalysis provides corporate-wide
support in Biological and Materials Microscopy to all divisions of Abbott
Laboratories. The facility houses two TEMs (a Philips CM12 STEM and a LEO
910), two SEMs (a Philips XL30-FEG and AMRAY 1830i), three EDXS systems, a
BioRad confocal scanning laser microscope, several fluorescent and light
microscopes (polarized light, DIC), and a Quantimet Image Analysis system.
We also have an Arcturus PixCell laser capture microdissection system and a
Becton Dickinson FACSCalibur flow cytometer. Microtomes include Reichert
Ultracut E and S ultramicrotomes, RMC 6000 XL cryoultrotome, Microm
histological microtome, and Microm HM500 cryostat.

Please send letters of application and resumes to:

Jane A. Fagerland, Ph.D.
Abbott Laboratories
D45M/AP31
200 Abbott Park RD.
Abbott Park IL 60064-6202

(847) 935-0104 voice
(847) 938-5027 fax
jane.a.fagerland -at-abbott.com



From daemon Tue Jun 06 23:34:52 2000



From: Christensen, Kim :      ChristeK-at-whiteoaksemi.com
Date: Tue, 6 Jun 2000 17:42:54 -0400
Subject: Re: T-Tool

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Sha Zhu,

The T-Tool is available from:
Precision TEM, Inc.
Santa Clara, CA
Ph: 408-980-8898
E-mail: liya-at-precisiontem.com

----------------------------------------------------
Kim Christensen
Failure Analysis Laboratory
White Oak Semiconductor
6000 Technology Boulevard
Sandston, Virginia 23150
Ph: 804 952 7307
Fax: 804 952 7902
Pager: 1 800 759 8888, pin# 130 3382
----------------------------------------------------



From daemon Wed Jun 07 08:28:06 2000



From: guerrier-at-ews.uiuc.edu, GUERRGI-at-mail.northgrum.com ()
Date: Tue, 6 Jun 2000 23:43:28 -0500
Subject: Help? Cleaning Optical Lenses

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Email: guerrier-at-ews.uiuc.edu, GUERRGI-at-mail.northgrum.com
Name: Gina Guerrieri

School: University of Illinois


Question: How do you clean a stereo zoom microscope? Specifically, how do
you clean the lenses with out removing them from the system?

---------------------------------------------------------------------------




From daemon Wed Jun 07 08:28:10 2000



From: Kristof Kovacs :      kris-at-almos.vein.hu
Date: Wed, 07 Jun 2000 08:37:32 +0200
Subject: Still alive?

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Dear Microscopists,
I'm just curious if something is wrong on my end or I'm unsuscribed against
my will. I did not receive any posting during the past two-three weeks, and
this is impossible in view of previously received 10-20 postings/day.
Is there any explanation?
Kris
Dr. Kristof Kovacs
Associate Professor
President, Hungarian Society for Microscopy
Phone: +36-(88)-421-684
Fax: +36-(88)-328-643
Mailing Address:
University of Veszprem, P.O.Box 158, Veszprem
H-8201 Hungary



From daemon Wed Jun 07 08:28:28 2000



From: Ian MacLaren :      maclariz-at-yahoo.co.uk
Date: Wed, 7 Jun 2000 08:27:57 +0100 (BST)
Subject: Ceramic cross-section preparation

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Dear all,
I'm currently having a few problems preparing good cross-section specimens
of ceramic thin films on LaAlO3 or NdGaO3 substrates.

I can cut them okay, but thinning them often results in cracking as the
thickness goes below 100 microns. I have a Gatan model 623 disk grinder and
currently have access to SiC abrasive papers to grit sizes of 1500 or 2000,
and also have the Gatan diamond polishing disks and specimen lapping kit.

The problems are as follows:
If we use the 1500 or 2000 grit SiC, we can often get the samples thin
enough, but with poor surface finish which needs to be improved with
dimpling (on both sides), we also risk cracking the sample as SiC papers
often contain imperfections.

Using the Gatan specimen lapping kit, I find that as the disk grinder is
altered to grind off a further 10 microns, the edge of the sample often
catches on the diamond disk and tears some of the diamond coating off,
leaving a lump on the surface which then risks catching the specimen and
cracking it.

I have one possible alternative approach which I used in Sweden last year
(with SiAlON ceramics) involving attaching the sample to a glass slide and
polishing it using diamond spray on non-absorbent paper. I may consider
trying this with these new materials.

Other ideas would be welcome, however, as would suggestions of how to
improve my present method. Please note that I do not have the budget to buy
any expensive new polishing equipment (such as a tripod polisher, for
instance), so the most welcome suggestions would be those that involve
improvements to current techniques, use of different types of polishing
consumables, etc..

I hope to hear several suggestions, both from users and from the companies
who producing polishing and lapping equipment. Why not post them with the
list so we can all benefit from the sharing of experience?

Best wishes

=====
Ian MacLaren
Beijing Laboratory of Electron Microscopy
Chinese Academy of Sciences, P.O. Box 2724
100080 Beijing
China
General Email: ian.maclaren-at-physics.org
Work (esp. large attachments): maclaren-at-image.blem.ac.cn

____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie



From daemon Wed Jun 07 08:28:29 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Wed, 07 Jun 2000 09:49:03 +0200
Subject: TEM - Searching for Review Article of Biological Applications

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Since I am usually more involved in material analysis I am a little lost on
the biological field. But now I am in the need of a good review article
concerning the application of (analytical) TEM to biological materials. I
am not looking for sophisticated latest developements but rather for basic
applications with some examples, if possible including some examples of
analytical TEM.

Hope to get some input :)

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Jun 07 08:28:35 2000



From: Gary Coulton :      g.coulton-at-ic.ac.uk
Date: Wed, 07 Jun 2000 12:09:24 +0100
Subject: ICHC 2000

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Dear Friend

ONLY 8 WEEKS TO GO UNTIL ICHC 2000 (3-8 Sept. York University, UK)

Abstracts are still welcome.

Register by the week or combinations of days.

For more details follow the links to the ICHC 2000 web-site below.

Best wishes

Gary Coulton
Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

TEL. ++44 020 7594 3190
FAX. ++44 020 7594 3022
e-mail g.coulton-at-ic.ac.uk


-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Cell Biology and Imaging Tools for the New Century"

September 3-8, 2000, York, United Kingdom

ICHC 2000 comprises 27 symposia addressing latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

8 weeks to go! Register for the week or by the day!

For further details go to http://www.med.ic.ac.uk/external/ichc_2000

See you there.



From daemon Wed Jun 07 08:28:37 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 7 Jun 2000 23:05:26 +1000
Subject: RE: Film vs Digital - the end?

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I was happy to leave things at that, but there are a couple of things that Mike
has raised that need an answer:
Your actual quote concerning Ansel Adams was "Having said that and looking at a
print of Ansel Adams, I am glad there is film, though!!" This is rather at odds
with the new proclamation
} " I mentioned Adams because I like his pictures. If he had taken them with
} a digital camera I would have liked them just as much.
Lots of people have made excellent compositions and many have make technically
superior prints. Adam's has excelled by consistently winning on both account.
Its a bit strange, but Mike I'll remind you why you his prints: It is not just
Adam's great compositions but also the incredible sharpness and tonal range,
which is only possible by huge data density and impossible with a 2.5mb
enlarged print. Digital not only has problems with occasional great demands on
print magnification (pixel size limitations) and lack of electron density (too
short exposures), but because TEM negs, like Adam's prints, are capable of a
terrific tonal range and resolution. An image appears better if theoretical
data minima are exceeded.

I have been a stickler for showing possible conflict of interest. I realize now
that I should have made a disclaimer along the way. I did not, simply because
it never occurred to me that I could have a real or imagined gain. My
aspirations are different, but since we are all subject to self-delusion I'll
add the reality:
We have very little mark-up on film and cannot export beyond New Zealand. For
Australian research institutions money has been very tight and equipment sales
have been low for some years. I doubt that at our exchange rate any digital TEM
cameras will be installed here during the next couple of years at least. I
think that my special friend Chuck is more likely to retain more film sales
than ProSciTech may have lost - if anybody was influenced either way.

The word belief is fitting for all of this. Whatever the arguments, I expect
people retain their "film versus digital" beliefs. Mike, we can lead a horse to
water, but just try to make it float on its back. When you do, send a picture
and I won't care if its digital.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, June 06, 2000 1:10 AM, Mike Bode [SMTP:mb-at-soft-imaging.com] wrote:
} Jim wrote:
}
} 'I have two replies and Mike my well have four replies to these.'
}
} I'll try. But I agree with you that we should let this thread die. jim,
} it seems you're a bit angy at me. If I unintentionally stepped on your
} toe I apologize. I did not expect that we would agree 100%, as you are
} selling film and I am involved in digital systems. I just hope that some
} other readers found some of this useful. I don't consider myself a
} "digital fanatic". This film vs. digital issue has many more facets,
} some of which we did not even touch, and which can be just as
} entertaining.
}
} Jim wrote:
}
} 'Mike reinforced and strengthened that argument, finishing with the note
} that he is glad for film when looking at prints by Ansel Adams.'
}
} Just for the record: I usually do not take a magnifying glass to photos.
} I mentioned Adams because I like his pictures. If he had taken them with
} a digital camera I would have liked them just as much. I also like some
} modern art paintings. That does not mean we should start drawing what we
} see in the microscopes rather than taking pictures ;-)
}
} Jim wrote:
}
} 'Incidentally, from the outset I cited increased "enlargability" to
} obtain high
} resolution TEM'
}
} Well, you said 'When great enlargements are required film is superior'.
} Perhaps I misunderstood. I thought, the term great enlargements referred
} to the microscope and meant 'small details', which you can take easier
} with a digital camera (no time delay between seeing something and taking
} an image, no mechanical vibration due to film movement, etc.). I have
} said many times before that film may be better if high resolution AND
} large field of view is required.
}
} Jim wrote:
}
} 'in any case: Show Sergey!'
}
} I'm in back-channel correspondence with him.
}
}
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Sunday, June 04, 2000 2:34 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Film vs Digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have two replies and Mike my well have four replies to these.
} 1. There has been a parallel discussion concerning resolution of
} film versus
} digital images. Clearly in raw power digital cannot compete since film
} has
} multi gigabyte capacity. I added to that thread that what matters is:
} does
} digital have enough power and that frequently it would. Mike reinforced
} and
} strengthened that argument, finishing with the note that he is glad for
} film
} when looking at prints by Ansel Adams.
} (Ansel Adams until about 30 years ago carted for decades large format
} cameras
} through US National Parks, especially Yosemite, producing fantastic
} landscape
} photographs and books)
} Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet
} film,
} probably rated at 400 ISO. The line resolution of such prints much
} exceeds our
} eyes' resolution, but still results in superior gradation and detail.
} TEM film,
} even when much enlarged has such details too.
} Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM,
} and
} because of the limited enlargability of light microscopy (concrete
} ceiling due
} to wavelengths) it's reasonable, but minimal for light microscopy. TEM
} can do
} and deserves better.
}
} Incidentally, from the outset I cited increased "enlargability" to
} obtain high
} resolution TEM, as one of films major advantages, since greater depths
} of field
} at moderate powers makes high powers through photo enlarging a desirable
}
} technique. The small additional magnification yielded by placing a
} digital
} camera lower in the column does not compensate. So greater
} "enlargability" of
} digitals would be desirable, but is limited by pixel size.
}
} 2 The thread was initiated by Sergey. He had problems visualising
} certain
} specimens in dark field. The use of his "beaut" digital TEM camera made
} things
} worse. I pointed out that the shorter exposure reduced the number of
} electrons
} forming the image, hence more noise. I believe that a good part of
} Mike's case
} will be settled in his favour when we hear "Eureka" from Sergey's lab. I
} don't
} doubt that much more can be done with digital now and that further
} improvements
} are on the way. Mike's "solution" may well be possible, but I don't
} believe its
} a snap; in any case: Show Sergey!
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
} wrote:
} }
} } Well, let's see:
} }
} } Jim wrote:
} }
} } Mike Bode would use multiple digital
} } exposures. The exposures could be layered and combined into one
} superior
} } image.
} } This image would be made up of more pixel and is formed by more
} } electrons and
} } so would be noise-free and hence could be further enlarged then
} } otherwise
} } possible. Perhaps.
} }
} } No, I did not talk about further enlargements. All I wanted to say is,
} } that a more noise-free image can be achieved by adding multiple
} images,
} } and that this also to some extent helps with drift of the sample
} during
} } acquisition.
} }
} } Jim wrote:
} }
} } How much time is required between exposures to transfer a minimum 10mb
} } image
} } per exposure?
} }
} } How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} } information is about 2.5 MB (uncompressed). We acquire about 10 of
} those
} } per second and transfer them across the PC bus to the display. Putting
} } them on them into Memory might add a few tenth of a second. Writing to
} } HD can be done after all images are acquired.
} }
} } Jim wrote:
} }
} } What would be the total time from focusing to the last exposure?
} } What about Z-drift in the interim requiring objective changes
} }
} } Why would we have to worry about that, if we don't have to worry about
} } that when taking the image on film? In fact, we could take care of
} this
} } by looking at the image between exposures and correct for z-drift.
} } However, as you said, that would add to the overall time and exposure.
} I
} } was comparing a normal dark field image taken on film at 8 seconds
} with
} } acquiring the same image on a "too sensitive" CCD camera by adding up
} 10
} } consecutive .8 second images. Why would the sample drift (in x, y or
} z)
} } substantially more in 8+delta seconds than in 8?
} }
} } Jim wrote:
} }
} } what about the total cost of this additional get-up
} }
} } That of course depends on the microscope and there is no general
} answer.
} } For example on a LEO 912 I believe the blanker is standard. The
} } additional cost to use an acquisition scheme like this with our
} software
} } is $0 plus perhaps a bit of time to write a small macro. On other
} } microscopes one might have to add a beam blanker and perhaps a control
} } mechanism for the beam blanker. But I would guess, that this cost is
} not
} } very high. All modern microscopes are computer controller anyway, so
} it
} } is most likely just a control command that needs to be sent to the
} } microscope over a serial port if the beam blanker is installed. Piece
} of
} } cake.
} }
} } Jim wrote:
} }
} } The mind boggles at a through focus series.
} }
} } You're right here. But I don't think we were talking about
} through-focus
} } series. Incidentally, we do through-focus series on light microscopes
} } and reconstruction routinely. Takes a few images at different focus
} (or
} } for a light microscope: stage) settings. The rest is done off-line.
} } Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} } agree that TEM is different here and much more complicated due to the
} } complicated Contrast Transfer Function. However, this could in
} principle
} } be sorted out.
} }
} } Jim wrote:
} }
} } Again, I don't doubt that there is now a large place for digital in
} TEM,
} } but
} } its no panacea.
} }
} } I also agree with you on that one. But using the additional computer
} } possibilities of digital imaging might take you further than expected.
} }
} } Michael
} }
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Friday, June 02, 2000 5:15 AM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Cc: 'jim-at-proscitech.com.au'
} } Subject: RE: Film vs Digital
} }
} }
} } The world is full of possible solutions, but are they practical.?
} } To produce high-resolution, dark-field or any others TEM images that
} } require
} } more electrons to form a clear image, Mike Bode would use multiple
} } digital
} } exposures. The exposures could be layered and combined into one
} superior
} } image.
} } This image would be made up of more pixel and is formed by more
} } electrons and
} } so would be noise-free and hence could be further enlarged then
} } otherwise
} } possible. Perhaps.
} } Beam blanking would largely save the specimen from beam damage and
} drift
} } could
} } be compensated for by matching up the digitals. Great.
} } How much time is required between exposures to transfer a minimum 10mb
} } image
} } per exposure? What would be the total time from focusing to the last
} } exposure?
} } What about Z-drift in the interim requiring objective changes and what
} } about
} } the total cost of this additional get-up. The mind boggles at a
} through
} } focus
} } series.
} } When pushing the limits a piece of film seems more effective, cheaper
} } and fa
} } ster.
} } Again, I don't doubt that there is now a large place for digital in
} TEM,
} } but
} } its no panacea.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} } wrote:
} } }
} } } No, it's not really a problem. It's been done with low density
} } } microscopy all the time. Granted, there are some technical aspects
} to
} } be
} } } overcome, but (and I can only speak for ourselves) we have done that
} } on
} } } a number of microscopes. You are of course correct, that 10 images
} at
} } } 0.8 seconds take longer than 8 seconds as the image has to be
} } } transferred, etc. BUT: that's what beam blankers are for. It is
} pretty
} } } straightforward to take an image at 0.8 seconds, then blank the beam
} } } very quickly before taking the next image. That way you get pretty
} } close
} } } to the 8 sec total exposure. If there is no beam blanker on the
} } } microscope, in most cases it can be added.
} } }
} } } I am not sure what you mean by "too sensitive". The cameras are
} } usually
} } } constructed so that 1 electron from the beam creates between a few
} } tenth
} } } to a few counts (these are all statistical data, of course). The
} well
} } } width divided by this sensitivity then determines, how many primary
} } } electrons are needed to fully expose one pixel. For example, if the
} } well
} } } width is 50,000 electrons and the sensitivity is 1 count/electron,
} one
} } } needs 50,000 primary electrons to fill the well. This translates
} into
} } } roughly a 0.4% statistical error.
} } }
} } } } From a practical standpoint: You can take images with most cameras
} } when
} } } the exposure meter on the microscope reads a couple of seconds
} without
} } } overexposing the camera. On the other hand, you can reduce the
} } intensity
} } } of the beam until you see single electron events.
} } }
} } } The one area where CCD cameras may be too sensitive is diffraction.
} } The
} } } normally huge intensity in the transmitted beam often leads to
} } } saturation. In CCDs this can lead to blooming (the intensity spills
} } over
} } } into neighboring pixels). This can be taken care of with special
} chips
} } } that have anti-blooming features, but this usually has some other
} } } drawbacks. Again, this can also be overcome somewhat with multiple
} } } exposures. Film behaves more civilized here, as it simply stops
} } } responding to the electrons, but this makes film more or less
} useless
} } } for quantitative measurements of diffraction patterns. I have done
} } } diffraction with CCDs many times and though it does require some
} } } tweaking, one can get very good results from them.
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } }
} } } -----Original Message-----
} } } From: jim [mailto:jim-at-proscitech.com.au]
} } } Sent: Wednesday, May 31, 2000 9:37 PM
} } } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } } Subject: RE: Film vs Digital
} } }
} } }
} } }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Yes, it could be done "in theory". Somebody would need to figure out
} } the
} } }
} } } software and perhaps modify the hardware. Then we would find that
} the
} } } total
} } } exposure of the specimen to the electron beam maybe a muliple of the
} } } film's
} } } exposure. Afterall, an 8 sec film exposure would not amount in
} digital
} } } to
} } } 10x0.8, but we would require considerable time in between exposures.
} } } Since the
} } } problems in the discussed circumstances are specimen movement and
} beam
} } } damage,
} } } it seems that taking multiple exposures is a poor option.
} } }
} } } Digital cameras are for some situation too sensitive to electron
} } } exposure.
} } } Cutting back on electrons is no option since its the electrons that
} } form
} } } the
} } } image in the first instance.
} } } Much easier in light microscopy . . . insert a neutral density
} } filter.
} } } Cheers
} } } Jim Darley
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } } www.proscitech.com
} } }



From daemon Wed Jun 07 08:28:37 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 7 Jun 2000 09:17:56 -0400
Subject: RE: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This sounds like a perfect application of the small angle cleavage
technique. You might want to contact Ray Tweston at Univ. of Illinois
because I think that we might have successfully prepared one of these while
I visited there. At any rate, the technique is very inexpensive and you can
prepare sample of superior quality. Get your hands on John McCaffrey and my
paper in the MRS TEM sample Prep IV book (vol 480). It has a detailed
description on how to do it. South Bay Technology sells the Microcleave kit
and you should contact them as well.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Ian MacLaren [mailto:maclariz-at-yahoo.co.uk]
} Sent: Wednesday, June 07, 2000 3:28 AM
} To: Microscopy list
} Subject: Ceramic cross-section preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear all,
} I'm currently having a few problems preparing good
} cross-section specimens
} of ceramic thin films on LaAlO3 or NdGaO3 substrates.
}
} I can cut them okay, but thinning them often results in
} cracking as the
} thickness goes below 100 microns. I have a Gatan model 623
} disk grinder and
} currently have access to SiC abrasive papers to grit sizes of
} 1500 or 2000,
} and also have the Gatan diamond polishing disks and specimen
} lapping kit.
}
} The problems are as follows:
} If we use the 1500 or 2000 grit SiC, we can often get the
} samples thin
} enough, but with poor surface finish which needs to be improved with
} dimpling (on both sides), we also risk cracking the sample as
} SiC papers
} often contain imperfections.
}
} Using the Gatan specimen lapping kit, I find that as the disk
} grinder is
} altered to grind off a further 10 microns, the edge of the
} sample often
} catches on the diamond disk and tears some of the diamond
} coating off,
} leaving a lump on the surface which then risks catching the
} specimen and
} cracking it.
}
} I have one possible alternative approach which I used in
} Sweden last year
} (with SiAlON ceramics) involving attaching the sample to a
} glass slide and
} polishing it using diamond spray on non-absorbent paper. I
} may consider
} trying this with these new materials.
}
} Other ideas would be welcome, however, as would suggestions of how to
} improve my present method. Please note that I do not have
} the budget to buy
} any expensive new polishing equipment (such as a tripod polisher, for
} instance), so the most welcome suggestions would be those
} that involve
} improvements to current techniques, use of different types of
} polishing
} consumables, etc..
}
} I hope to hear several suggestions, both from users and from
} the companies
} who producing polishing and lapping equipment. Why not post
} them with the
} list so we can all benefit from the sharing of experience?
}
} Best wishes
}
} =====
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences, P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}



From daemon Wed Jun 07 08:57:58 2000



From: Judith C. Yang :      jyang-at-engrng.pitt.edu (by way of Nestor J.
Date: Wed, 7 Jun 2000 08:34:48 -0500
Subject: post-doc opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Postdoctoral Position Immediately Available
In situ UHV-TEM of Nanoparticle Reactions and Metal Oxidation

Materials Research Laboratory, University of Illinois at
Urbana-Champaign
and University of Pittsburgh

A postdoctoral position is immediately available in the Materials
Research
Laboratory at the University of Illinois at Urbana-Champaign in the area
of
nano-reactions and in situ UHV-TEM. This position is jointly sponsored
between
Professor Robert Averback (University of Illinois at Urbana-Champaign)
and
Professor Judith Yang (University of Pittsburgh).

The research project is two-fold: 1. Surface oxidation kinetics of
metals
and 2. Nanoparticle reactions/sintering. The research project is to
combine the unique experimental information obtainable from in situ
UHV-TEM
and compare with
theoretical models of these nanoscale reactions. The position requires
a
PhD in physical sciences/engineering. Hands-on experience in TEM
techniques
is highly desirable. The position is open to all qualified candidates
and
has an anticipated duration of 2 years. If you are interested in this
opportunity, please send a resume and names of three references to the
address below.


Dr. Judith C. Yang
Assistant Professor
Dept. of Materials Science & Engineering
848 Benedum Hall
University of Pittsburgh
Pittsburgh, PA 15261

Tel: (412) 624-8613
Fax: (412) 624-8069
jyang-at-engrng.pitt.edu




From daemon Wed Jun 07 08:57:59 2000



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Wed, 7 Jun 2000 08:37:34 -0500
Subject: re: TEM - Searching for Review Article of Biological Applicatio

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I can reccomend you the following books:
1. D.C.Joy, A.D.Romig and J.I.Goldstein, "Principles of analytical
electron microscopy", Plenum Press, 1989;
it contains three chapters dedicated to bilogy: chapter 6, 12 and 13
2. J.J.Hren, J.I.Goldstein and D.C.Joy, "Introduction to AEM",
Plenum Press, 1979;
it contains chapters dedicated to biology.
I hope this helps.

Corneliu Sarbu
Dept.MTM
KULeuven
Belgium

- - - - - - - - - - - - - - Original Message - - - - - - - - - - - - - -
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




Since I am usually more involved in material analysis I am a little lost on
the biological field. But now I am in the need of a good review article
concerning the application of (analytical) TEM to biological materials. I
am not looking for sophisticated latest developements but rather for basic
applications with some examples, if possible including some examples of
analytical TEM.

Hope to get some input :)

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin


- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -




From daemon Wed Jun 07 18:36:38 2000



From: Kim Pierson :      piersokw-at-uwec.edu
Date: Wed, 07 Jun 2000 09:03:26 -0500
Subject: JEOL 2010 LaB6 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Question:
What is the proper procedure for increasing the filament current on a 2010
with a LaB6?
How fast can you go up?
Should the current be increased at a linear rate or should the rate be
tapered off as you reach saturation?
The manual suggests 30 sec/graduation while the service techs suggest a
much slower rate.

Thanks,
Kim
**************************************************************
Kim W. Pierson, Ph.D.
Dept of Physics & Astronmoy
University of Wisconsin-Eau Claire
(715) 836-5009
FAX 836-3955



From daemon Wed Jun 07 18:36:38 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 7 Jun 2000 10:41:19 -0400
Subject: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ian:

I have a few comments for you here that may help. This is a little touchy
as I don't want to knock my competitor's equipment. The Model 623 Disk
Grinder that you have is going to cause problems as you describe because of
it's design. The way that grinder works is that you use the dial on the
top to make your sample extend below the base of the grinder. When you
begin polishing, the entire weight of the grinder plus whatever weight you
are applying by hand is being transferred to your very thin 3mm diameter
sample. In addition to the additional weight, you can tend to apply too
much pressure on one side of the sample which would cause the sample to
"catch" on the diamond film as you describe.

We manufacture a series of polishing fixtures that are designed to prevent
these problems. They are gravity feed fixtures. On these fixtures, the
dial gauge at the top simply sets the amount of material that will be fed
into the abrasive film. The only weight your sample sees is the weight of
the central piston (with some of our fixtures, this weight can be
counterbalanced to approach zero). The weight of the rest of the fixture
is never transferred to the sample. Also, the sample surface that is being
polished is always co-planar with the base of the polishing fixture. This
ensures that you cannot apply uneven pressure and that you won't experience
the "catching" that you described.

Now the good news. There is one of these fixtures (Model 150) already in
use in your facility. There is also the Tripod Polisherš, Model 920
Lapping & Polishing Machine, Model 850 Wire Saw and Model 650 Diamond Wheel
Saw. By separate e-mail,I will give you the details on where these are
located and who you should contact to access them.

I also saw Scott Walck's post on the Microcleave technique. I will include
a PDF of the paper he was talking about by separate e-mail.

If you have any questions or if I can be of any additional assistance,
please contact me directly off-line. I hope this helps.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 7:20:30 AM on 06/07/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by INTERNET:ian.maclaren-at-physics.org
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,
I'm currently having a few problems preparing good cross-section specimens
of ceramic thin films on LaAlO3 or NdGaO3 substrates.

I can cut them okay, but thinning them often results in cracking as the
thickness goes below 100 microns. I have a Gatan model 623 disk grinder
and
currently have access to SiC abrasive papers to grit sizes of 1500 or 2000,

and also have the Gatan diamond polishing disks and specimen lapping kit.

The problems are as follows:
If we use the 1500 or 2000 grit SiC, we can often get the samples thin
enough, but with poor surface finish which needs to be improved with
dimpling (on both sides), we also risk cracking the sample as SiC papers
often contain imperfections.

Using the Gatan specimen lapping kit, I find that as the disk grinder is
altered to grind off a further 10 microns, the edge of the sample often
catches on the diamond disk and tears some of the diamond coating off,
leaving a lump on the surface which then risks catching the specimen and
cracking it.

I have one possible alternative approach which I used in Sweden last year
(with SiAlON ceramics) involving attaching the sample to a glass slide and
polishing it using diamond spray on non-absorbent paper. I may consider
trying this with these new materials.

Other ideas would be welcome, however, as would suggestions of how to
improve my present method. Please note that I do not have the budget to
buy
any expensive new polishing equipment (such as a tripod polisher, for
instance), so the most welcome suggestions would be those that involve
improvements to current techniques, use of different types of polishing
consumables, etc..

I hope to hear several suggestions, both from users and from the companies
who producing polishing and lapping equipment. Why not post them with the
list so we can all benefit from the sharing of experience?

Best wishes

=====
Ian MacLaren {



From daemon Wed Jun 07 18:36:39 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Wed, 7 Jun 2000 09:50:51 -0600 (Hora est‡ndar de MŽxico)
Subject: plant vesicles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm trying to do immunogold studies on a membrane protein in a preparation
of plant vesicles (from minus 80ŒC storage). I did an Epon embedding with
standard procedure (glut and osmium) to check the ultrastructure and got
decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
paraformaldehyde, without osmium) looks like a disaster, with granular
material, vague membranish-like structures and whorls of membranes. Does
anyone have any tips for LR White preps of vesicles, or any other ideas
for embedding (resins, fixing, dehydration, embedding) to favour the
immunogold process.

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Wed Jun 07 18:36:47 2000



From: Michelle.Taurino-at-aventis.com
Date: Wed, 7 Jun 2000 11:59:26 -0500
Subject: TEM - Searching for Review Article of Biological Applications

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Petra-

A book that I have found to be very resourceful is
Biomedical Electron Microscopy by Arvid B. Maunsbach and Bjorn Afzelius.

Hope this is useful to you as well.
Regards-
Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: Petra Wahlbring [mailto:wahlbrin-at-crpgl.lu]
Sent: Wednesday, June 07, 2000 3:49 AM
To: microscopy-at-sparc5.microscopy.com




Since I am usually more involved in material analysis I am a little lost on
the biological field. But now I am in the need of a good review article
concerning the application of (analytical) TEM to biological materials. I
am not looking for sophisticated latest developements but rather for basic
applications with some examples, if possible including some examples of
analytical TEM.

Hope to get some input :)

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Jun 07 18:36:48 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 7 Jun 2000 10:01:04 -0700
Subject: Re: SEM: agency wants SEM images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} My SEM facility has just been approached, and in the interest in
} providing what they want for a price based on an average, can I query
} the SEM community as to what they charge for commercial release of
} their SEM imagery. I'm looking for $/image, but if you charge on any
} type of sliding scale, I'd be interested in that too. I believe as
} well I shouldn't be undercutting commercial facilities, so I'm
} especially interested in these numbers.
} Reply to me direct, and I'll respond back to the list with the
} average, max and min, and no names.

shAf-

Please start with an inquiry to your university administration; you might
save yourself a lot of grief. When I was running an interdepartmental lab
at U.C. Berkeley, there were very specific university-wide rules that I was
required to follow for such usage.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Jun 07 18:36:56 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Wed, 7 Jun 2000 13:02:55 -0700
Subject: RE: JEOL 2010 LaB6 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Kim:

We have a 2010 and our procedure is to bring the filament dial (labeled
off-10) to position #3. Then we go for coffee. After 20 min. or so we raise
it to # 4, after a couple of minutes to #5 etc. until we get to the stop (
in our case we have it at about 7 1/2). Even at this point we have filament
drift for quite some time, so , we are not ready to take pictures for
another 1/2 hr or so. By the way, we have the bias settings at 7 and 7 which
is high. I would be interested in knowing what your settings are.

I hope this helps

Jordi Marti




From daemon Wed Jun 07 18:36:56 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 7 Jun 2000 20:48:55 +0100
Subject: Re: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 14:19 +0200 3/6/00, Jonathan Barnard wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

My understanding is as follows ....

There are different damage mechnisms at different voltages.

At lower voltages ( {200 kV) the main damage mechanism is, in effect,
specimen heating. And, the lower the voltage, the greater the
interaction (elastic scattering) with the specimen - so more damage
but greater contrast, the main reason users looking at resin sections
prefer lower voltages.

Increasing the voltage reduces specimen damage and tends to improve
resolution but at the expense of contrast. As the interest in
biological TEM moves to molecular biology, resolution is more
important. But, at high resolution, phase contrast becomes the
dominant factor in producing image constrast and phase constrast can
be significantly improved - without loss of resolution - by using
highly coherent FEG sources.

At voltages between 300kV and 400 kV, the energy of the electron
becomes sufficient to cause "direct" radiation damage - atoms are
displaced. At this point, specimen damage rates increase. The
particular voltage depends on the amtomic number of your specimen.

Damage rates can also be reduced by cooling. in particular, cooling a
specimen to liquid helium temperatures allows significantly greater
electron exposure before damage occurs.

So, for optimum imaging of molecular specimens you need 300 kV and a
FEG gun plus a liquid helium stage.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Wed Jun 07 18:37:00 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 7 Jun 2000 17:11:48 -0500
Subject: RE: NEED HELP WITH EDS ON A JEOL 100S

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For EDS checking you can use specimen
from Cu or Al foil so that it
will have any angle, including 30-60
degrees. But I am afraid that if there
is no spectra at all with 10 degree
tilt then a problem is more complicated.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: "MGMANDERS-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"MGMANDERS-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Sunday, June 04, 2000 10:41 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: NEED HELP WITH EDS ON A JEOL 100S
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} A fellow colleague has bought a JEOL 100s and wants and
} needs EDS X-ray
} Analysis. He's mounted his horizintal EDS detector , but can
} not get sample
} X-rays from the speciman. Contacting JEOL he found the
} problem to be a tilt
} problem. The 10 degree tilt from the normal SEG only tilts
} 10 degrees and a
} 30 to 60 degree tilt is necessary. At one time I was told
} that special
} sample holders and or double gap pole pieces were availabe.
} He wishs to find
} any one with a 100s for parts needed or information which
} would allow X-ray
} analysis. Please reply to mgmanders-at-aol.com or the list server.
}
} Mike Manders
}
}
}



From daemon Wed Jun 07 20:58:24 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Wed, 7 Jun 2000 21:49:08 -0500
Subject: TEM: Set-up, operating and facilities costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List Members:

We have the opportunity to acquire a TEM, but budget constraints mean that
have to consider the cost of acquiring and operating this instrument
carefully against alternatives. We plan to use it principally to study the
morphology, behavior and size distribution of colloidal gold particles and
other types of metal nanoparticles.

Can anyone give any information on what the maintenance, operating and
facilities requirements and costs would be for this instrument, or point me
to a good source? What renovations would be necessary to accommodate it -
darkroom, ancillary equipment, cooling, water and power supplies, darkroom
facilities? How much should we budget for supplies and consumables? Since
we have no-one currently trained to use it, how long would it take to
learn, and how much maintenance (time, supplies, contracts) would it
require?

Thanks in advance,

Rick Powell


**********************************************************************
* - PLEASE NOTE OUR NEW ADDRESS - EFFECTIVE FEBRUARY 15, 2000 - *
* *
* NANOPROBES, Incorporated | US Toll-free: (877) 447-6266 *
* 95 Horse Block Road | Tel: (919) 510-0590 *
* Yaphank, NY 11980-9710, | Fax: (919) 510-0590 *
* USA | rpowell-at-nanoprobes.com *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************




From daemon Wed Jun 07 23:18:25 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 07 Jun 2000 23:34:37 -0400
Subject: Re: UV optics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don,

Both Zeiss and Leica have long-standing reputations for quartz optics with
transmission into the UV (typically, down to about 220nm). You are going to
need more than just the objective; the binoc also needs to have a UV
transmitting prism. A tip: inquire about microscopes used for
microspectrophotometry or in semiconductor applications. From the
biological/biomed realm: microscopes used for Fura or Indo studies.

Try Tom Calahan at Zeiss (914-681-7733) and Jan Hinsch at Leica/Allentown
(201-236-5905).

Nikon has also been agressive in lens development, but I haven't had direct
experience with any UV optics. Call Stan Schwartz (516-547-8529). ... and
at Olympus: Reinhard Enders (516-844-5000).

Good hunting!
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suitee 102
Springfield, MA 01118

PH: 413-746-6931 FX: 413-746-9311 email: mme-at-map.com

Customized, on-site short courses in all areas of microscopy, sample prep,
and image analysis.
"Why didn't they teach us that sooner?" Probably because no one called MME!






At 03:27 PM 6/6/00 -0400, donald j marshall wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 07 23:18:25 2000



From: Barbara Foster :      mme-at-map.com
Date: Thu, 08 Jun 2000 00:12:10 -0400
Subject: Re: digital OMs for an EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Edorado,


Suggest you buy a good upright stand which you can upgrade as your needs
develop. I strongly suggest a combination of both transmitted and
reflected light options.


Your feelings re: investing in the microscope vs. a high end camera at
this stage are well founded.


Re: contrast techniques... I think that you might find Hoffman Modulation
Contrast a better bet for what you are doing than Phase. Hoffman can be
used singly for looking at surfaces (ex: your coatings) or in combination
with polarized light. It is a good complement to DIC, which won't work if
your powders, etc. respond to Pol.


All of the "big 4" (Olympus, Nikon, Leica, and Zeiss) have good
equipment. The issues I would add to your shopping list might include
how comfortable you feel operating a specific microscope (like trying on
a coat or test-driving a new car) and how supportive your local dealer or
representative is.


Your comment re: EM was interesting.... I have been doing a lot of work
over the past 6 months with commercial companies who look at many of the
applications you cited. Universally, they were astounded at the
information they could get from the Light Microscope, quickly and with
minimal sample prep. I am currently on assignment teaching a group of
very competent EM people about Light Microscopy... and they are equally
amazed. All by themselves, they came to the conclusion that they should
take a look first with the Light Microscope and then, if necessary, go
the SEM. (I added that they should also take a quick look with the stereo
first).


By the way, you may be interesting in "Optimizing Light Microscopy", both
as a reference for your lab and a text for your students. Details are on
our website.


Happy shopping!

Barbara Foster

Microscopy/Microscopy Education

125 Paridon Street, Suitee 102

Springfield, MA 01118


PH: 413-746-6931 FX: 413-746-9311 email: mme-at-map.com

www.MME-Microscopy.com/education


Customized, on-site short courses in all areas of microscopy, sample
prep, and image analysis.

"Why didn't they teach us that sooner?" Probably because no one called
MME!



At 09:47 AM 6/7/00 +0200, Bemporad, Edoardo wrote:

} } } }

{excerpt}

{fontfamily} {param} Arial {/param} {smaller} I am going to buy one ore two OM
for our EM lab (XL30 and CM120), trying to convince myself that one brand
is better than the other (quality/price rate included in the
evaluation!).

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} In our lab we do mainly
metallographic analysis, interface studies of wear resistant coatings,
and catalytic powders characterization, but I guess that the OM will be
used for a wider range of investigation (W/O and O/W emulsions, asbestos,
..) and for didactical scopes too.

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} So EPI and DIA illuminations,
BF, DF, NIC, phase contrast, pol, I don't think we will need
fluorescence; forgotten something?

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} What about an inverse
microscope? or always better two standard ones? {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} I have about 35K$ budget to make
everybody happy (research group and students), and my position is to
prefer spending on the microscope (or microscopes) rather than on a
high-level digital camera. I read some threads about it here and I think
something like a Nikon coolpix 990 (if it will works! :-) ) will be
enough. (other opinions or point of views?)

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} I do not have a so deep
experience in OM but I was wandering if there are some key feature to
keep in mind for an equipment that will be used in a EM lab, considering
that where I will not able to go with the OM (depth of field, resolution)
I can use our EM!

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Anybody have suggestion (base
set, optional...)? {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} I will post a report with the
collected hints; Thank You . {/smaller} {/fontfamily}


{fontfamily} {param} Arial {/param} {smaller} Dr. Eng. Edoardo Bemporad, Ph.
D. {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Assistant Professor of Materials
Science {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} University of Rome "Roma Tre"
(Italy) {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Dipartimento di Ingegneria
Meccanica e Industriale {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} (Department of Mechanical and
Industrial Engineering) {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Via Vasca Navale 79 - 00146
Rome, Italy {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Tel:
+39 {/smaller} {/fontfamily} {fontfamily} {param} Arial {/param} {smaller} 06
5517.3293 {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Fax:
+39 {/smaller} {/fontfamily} {fontfamily} {param} Arial {/param} {smaller} 06
5517.3256 {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} LIME Lab Tel:
+39 {/smaller} {/fontfamily} {fontfamily} {param} Arial {/param} {smaller} 06
5517.3200 {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} LIME Web Site:
http//materials.dimi.uniroma3.it/lime {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} E-Mail:bemporad-at-uniroma3.it
{/smaller} {/fontfamily}


{/excerpt} { { { { { { { {




{/x-rich}



From daemon Wed Jun 07 23:18:26 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 07 Jun 2000 23:47:15 -0400
Subject: Re: Help? Cleaning Optical Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gina,

Most of the lenses should come off, either directly or with the help of a
small Allen wrench.

My favorite approach to lens cleaning: "Puff, Huff, and Swirl"....
1. Puff off any debris or rough material which might scratch the lens using
either a puffer (available from photographic supply houses) or just
"puffing" the dry air from your mouth with your cheeks (easier to demo than
explain).
2. Huff on the lens, using the warm, moist air from deep in your lungs, to
deposit a fog of moisture on the lens then
3. Quickly and gently, remove the fog with a clean Q-tip (100% cotton ) or
lens tissue (NOT Kim-Wipe!), starting at the middle of the lens and
continuing in a spiral to the outer edge. Do not use the same area on the
swab or tissue more than once; they will collect debris which can scratch
the delicate coating.
4. If the dirt is persistent (ex: mascara, fingerprint, oily residue), dip
the tip of a cotton swab in a good lens cleaning solution (also available
at photo supply houses), shake off any excess, then repeat the "swirl" step.

I recently had a client who had a neat moist towelette made by Uvex. It
worked really well, even on oil. I will have to find the contact info, so
if you are interested, please email me off line.

Best regards,
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suitee 102
Springfield, MA 01118

PH: 413-746-6931 FX: 413-746-9311 email: mme-at-map.com

Customized, on-site short courses in all areas of microscopy, sample prep,
and image analysis.
"Why didn't they teach us that sooner?" Probably because no one called MME!


At 11:43 PM 6/6/00 -0500, wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 08 08:30:15 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 8 Jun 2000 08:37:05 +0100 (BST)
Subject: Re: JEOL 2010 LaB6 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Kim,

The real question is why should you increase the LaB6 heating current
slowly? As I understand it there are two reasons. Firstly if the tip is
new, the gun has been serviced or the gun has not been run at 200kV for
some time it is important to run up slowly to ensure that you do not get
any outgassing from the tip or other gun components that might cause a
flashover and possible damage. Secondly if the machine is regularly used
at 200kV and the gun is is good condition (normal case) then you want to
avoid any thermal shock to the LaB6 tip which could cause damage or
misalignment. The same applies to cooling the tip down after use.

In the first case I would certainly take several 10s of minutes while
carefully watching the vacuum gauge and the emission current meter (and HT
stability if you are connected to an oscilloscope). Exact time would
depend on the condition but maybe 30 minutes to heat a new tip and 2
minutes for a normal tip in good condition. I would take a minute to cool
down a tip.

In both cases the greatest heating effect is a square of the control
position (power is proportional to V^2 or I^2) and this should be taken
into acount. I heat to about 3.5 in 25 to 30 seconds then decrease my
heating speed as I approach the stop (set at 5 in my case, bias 5.5). The
particular setting will depend on the type of LaB6 tip used, the wenhelt
to tip distance and how you run the emission. We use 5uA emission with the
tip slightly undersaturated as it seems to give good results and
reasonable long life at the high mags that we use. For the smallest probes
we may desaturate further to reduce the probe size.

I am aware from visitors that we have from other sites that this is
quicker than many people but it seems impractical to spend 30 minutes to
run up the tip every time you want to change a specimen when we want to
look at several in a session. Our tip life des not seem to be any worse
than those who take much longer.

Regards,
Ron



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Question:
} What is the proper procedure for increasing the filament current on a 2010
} with a LaB6?
} How fast can you go up?
} Should the current be increased at a linear rate or should the rate be
} tapered off as you reach saturation?
} The manual suggests 30 sec/graduation while the service techs suggest a
} much slower rate.
}
} Thanks,
} Kim
} **************************************************************
} Kim W. Pierson, Ph.D.
} Dept of Physics & Astronmoy
} University of Wisconsin-Eau Claire
} (715) 836-5009
} FAX 836-3955
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Thu Jun 08 08:30:15 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 8 Jun 2000 11:09:37 +1000
Subject: RE: plant vesicles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LR White won't look as good as Spurr's, but membrane whorls are indicative of
insufficient fixation. Os is the better lipid (therefore membrane) fixative,
since you cannot use Os, you may need to increase the time and or concentration
of GA.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, June 08, 2000 1:51 AM, Mark West
[SMTP:mwest-at-ifcsun1.ifisiol.unam.mx] wrote:

}
} Hi,
}
} I'm trying to do immunogold studies on a membrane protein in a preparation
} of plant vesicles (from minus 80oC storage). I did an Epon embedding with
} standard procedure (glut and osmium) to check the ultrastructure and got
} decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
} paraformaldehyde, without osmium) looks like a disaster, with granular
} material, vague membranish-like structures and whorls of membranes. Does
} anyone have any tips for LR White preps of vesicles, or any other ideas
} for embedding (resins, fixing, dehydration, embedding) to favour the
} immunogold process.
}
} Thanks,
}
} Mark
}
}
}
}
}
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************
}



From daemon Thu Jun 08 08:30:21 2000



From: Per Hšrstedt :      per.horstedt-at-pathol.umu.se
Date: Thu, 08 Jun 2000 13:44:10 +0200
Subject: Re: Still alive?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Kris,
same thing happened to me, but re-subscribing took care of the problem.
Mailed to listserver, but no respons at all.


} Dear Microscopists,
} I'm just curious if something is wrong on my end or I'm unsuscribed against
} my will. I did not receive any posting during the past two-three weeks, and
} this is impossible in view of previously received 10-20 postings/day.
} Is there any explanation?
} Kris
} Dr. Kristof Kovacs
} Associate Professor
} President, Hungarian Society for Microscopy
} Phone: +36-(88)-421-684
} Fax: +36-(88)-328-643
} Mailing Address:
} University of Veszprem, P.O.Box 158, Veszprem
} H-8201 Hungary

Yours sincerely

Per Hšrstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of UmeŒ
S-90187 UmeŒ
Sweden

phone int-46-90-7851541
fax int-46-90-7851215



From daemon Thu Jun 08 08:30:24 2000



From: prenitzer,brenda s :      prenitzer-at-lucent.com
Date: Thu, 8 Jun 2000 08:54:59 -0400
Subject: Re: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Literature indicates that specimen damage due to heating decreases with
increasing accelerating voltage; however, there is a trade off because at
higher voltages materials fall victim to knock-on and sputtering damage. A
very good overview (with references) of specimen/beam interactions and beam
damage can be found on PP 49-55 "Transmission Electron Microscopy" by
Williams and Carter 1996 Plenum Press ISBN: 0-306-45342-X

Brenda I. Prenitzer, Ph.D.
Member of Technical Staff
Cirent Semiconductor (Lucent Technologies)
9333 S. John Young Parkway
6D-Lab
Orlando, FL 32819-8612

Phone: 407 371 7108
Fax: 407 371 6999

prenitzer-at-lucent.com
bsp101-at-worldnet.att.net


-----Original Message-----
} From: Larry Stoter [mailto:LPS-at-teknesis.demon.co.uk]
Sent: Wednesday, June 07, 2000 3:49 PM
To: Jonathan Barnard; microscopy-at-sparc5.microscopy.com


At 14:19 +0200 3/6/00, Jonathan Barnard wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

My understanding is as follows ....

There are different damage mechnisms at different voltages.

At lower voltages ( {200 kV) the main damage mechanism is, in effect,
specimen heating. And, the lower the voltage, the greater the
interaction (elastic scattering) with the specimen - so more damage
but greater contrast, the main reason users looking at resin sections
prefer lower voltages.

Increasing the voltage reduces specimen damage and tends to improve
resolution but at the expense of contrast. As the interest in
biological TEM moves to molecular biology, resolution is more
important. But, at high resolution, phase contrast becomes the
dominant factor in producing image constrast and phase constrast can
be significantly improved - without loss of resolution - by using
highly coherent FEG sources.

At voltages between 300kV and 400 kV, the energy of the electron
becomes sufficient to cause "direct" radiation damage - atoms are
displaced. At this point, specimen damage rates increase. The
particular voltage depends on the amtomic number of your specimen.

Damage rates can also be reduced by cooling. in particular, cooling a
specimen to liquid helium temperatures allows significantly greater
electron exposure before damage occurs.

So, for optimum imaging of molecular specimens you need 300 kV and a
FEG gun plus a liquid helium stage.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail:
larrys-at-jeoleuro.com



From daemon Thu Jun 08 08:47:07 2000



From: Peter Bond :      P.Bond-at-plymouth.ac.uk
Date: Thu, 8 Jun 2000 08:31:38 -0500
Subject: LRWhite flat embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

I remember a message some time ago, I think, suggesting a way to flat
embed in silicon molds using LRWhite resin. How the top was sealed
escapes me. Could anyone with a better filing system and/or memory
please send me a message or a reference on this.

Thanks.

Pete
--
Peter Bond
Plymouth Electron Microscopy Unit
University of Plymouth
Drake Circus
Plymouth
Devon
UK PL4 8AA
Tel/Fax: 01752 233092
email: pbond-at-plymouth.ac.uk




From daemon Thu Jun 08 08:47:08 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 8 Jun 2000 08:35:01 -0500
Subject: SEM/TEM available...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

If you are looking for an inexpensive TEM and/or SEM, The University of
Portland has two such units you might be interested in.

1) Zeiss EM9s2, transmission electron microscopy, with additional high
voltage tank, replacement for vacuum tubes, fuses, new filaments, lots of
film cassettes, and film, original schematics, and operation manuals.

2) ETEC AutoScan, scanning electron microscope, with 60 and 90 degree
stages, several reconditioned column liner tubes, new apertures, new YAG
scintillator in original container, original schematics and operating
manuals, plus, video tapes on operation and use. This instrument was
original manufactured for INTEL and has a 50kV power supply.

Both units are operational!

Any interested parties or individuals can contact me off the server.

Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203 USA

(503) 413-5391





From daemon Thu Jun 08 08:52:47 2000



From: Siegel, Stephen :      ssiegel-at-EMSL.com
Date: Thu, 8 Jun 2000 09:56:58 -0400
Subject: TEM Analyst Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EMSL Analytical Inc. (Westmont, NJ) is interested in hiring a Transmission
Electron Microscopy Analyst (TEM) for its NJ Corporate Office. EMSL is the
world leader in asbestos analysis since 1981 with over 20 laboratory
locations worldwide.

Responsibilities include the preparation and analysis of air, bulk, and
water samples submitted by clients for asbestos content and other specialty
asbestos projects. College Degree in Materials Science or related field and
past experience with electron microscopy analysis preferred. . The ideal
candidate would be a detail orientated individual able to follow lab
protocols and procedures and able to work in a fast paced environment.

Full benefits package with salary commensurate with experience. Interested
individuals send resume and salary requirements to Stephen Siegel, CIH
(ssiegel-at-emsl.com) or fax to 856-858-4960.

Stephen Siegel
Stephen Siegel, CIH
Asbestos Lab Manager
EMSL Analytical, INC
107 Haddon Avenue
Westmont, NJ 08108
Phone:800-220-3675x1209 Fax:609-858-4960
email:ssiegel-at-emsl.com



From daemon Thu Jun 08 09:00:29 2000



From: garyliechty-at-att.net
Date: Thu, 8 Jun 2000 08:50:50 -0500
Subject: Re: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ian,

Your main problem is with the use of SiC for grinding
and thinning.

Silicon carbide paper should not be used to prepare any
ceramic material, it is not hard enough to cut it
efficiently without damaging the substructure. SiC
becomes dull quite rapidly, with ceramic a dull abrasive
actually cracks the structure and causes pullout and
excessive chipping. You are compounding your problem
with the weight of the tool being used as well. While
the disc grinder is a well designed product and very
useful, it is quite heavy for samples of certain
thicknesses not to mention the additional pressure you
are applying is not helping any.

The solution is to use Diamond Lapping Film and apply
some Diamond Extender at the final stages of thinning to
reduce the surface tension between the water and the
sample. This reduces the sheer stress being applied to
the sample and will drastically reduce the possibility
of cracking.

Should you have interest, we offer a polishing machine
"The MultiPrep System" that allows you to prepare the
sample without the possibility of the tool being
misaligned or mishandled (tilted on edge) during prep.
Only the sample makes contact with the abrasive and the
plane of polish remains in tact throughout the
polishing/grinding process. A dial indicator (1 micron
increments) allows you to preset a known amount of
material to be removed and there is no operator
intervention until the sample is done. The amount of
force applied to the sample is constant regardless of
the operator.

If you wish to get more information about the MultiPrep
System, you may visit our website:
www.alliedhightech.com or contact me in person at 310-
635-2466.

Sincerely,

Gary Liechty
Manager, Technical Products
Allied High Tech Products, Inc.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} I'm currently having a few problems preparing good cross-section specimens
} of ceramic thin films on LaAlO3 or NdGaO3 substrates.
}
} I can cut them okay, but thinning them often results in cracking as the
} thickness goes below 100 microns. I have a Gatan model 623 disk grinder and
} currently have access to SiC abrasive papers to grit sizes of 1500 or 2000,
} and also have the Gatan diamond polishing disks and specimen lapping kit.
}
} The problems are as follows:
} If we use the 1500 or 2000 grit SiC, we can often get the samples thin
} enough, but with poor surface finish which needs to be improved with
} dimpling (on both sides), we also risk cracking the sample as SiC papers
} often contain imperfections.
}
} Using the Gatan specimen lapping kit, I find that as the disk grinder is
} altered to grind off a further 10 microns, the edge of the sample often
} catches on the diamond disk and tears some of the diamond coating off,
} leaving a lump on the surface which then risks catching the specimen and
} cracking it.
}
} I have one possible alternative approach which I used in Sweden last year
} (with SiAlON ceramics) involving attaching the sample to a glass slide and
} polishing it using diamond spray on non-absorbent paper. I may consider
} trying this with these new materials.
}
} Other ideas would be welcome, however, as would suggestions of how to
} improve my present method. Please note that I do not have the budget to buy
} any expensive new polishing equipment (such as a tripod polisher, for
} instance), so the most welcome suggestions would be those that involve
} improvements to current techniques, use of different types of polishing
} consumables, etc..
}
} I hope to hear several suggestions, both from users and from the companies
} who producing polishing and lapping equipment. Why not post them with the
} list so we can all benefit from the sharing of experience?
}
} Best wishes
}
} =====
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences, P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}




From daemon Thu Jun 08 18:12:44 2000



From: Arey, Bruce W :      bruce.arey-at-pnl.gov
Date: Thu, 08 Jun 2000 07:24:57 -0700
Subject: JEOL 840 SEM Vacuum Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have JEOL 840 SEM that has develop a leak at the specimen chamber door
interface and will not hold a vacuum. We have changed the o-ring around the
door, look for nicks, scratches any other damage around the door interface, none
found. We have also check the roughing valve (V4, LV3 and the pressure relief
valve) for leaks. Pressure holds in the gun area. The large door is only opened
when we have to replace or add a new attachment to the chamber and thats about
once a year. We have a vacuum specimen exchange port to enter and retrieve
samples. We have also check that seal and it also checks out OK.
We have had 2 JEOL engineers take a look at the system and at this point no luck
finding the cause or solution. You can respond to me off-line at
bruce.arey-at-pnl.gov
Thanks

Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308





From daemon Thu Jun 08 18:12:44 2000



From: Yves Giroux :      ygiroux-at-istar.ca
Date: Thu, 08 Jun 2000 10:34:27 -0400
Subject: PGT parts for anyone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi:

I am about to tear the guts out of an old PGT System4 console. I need some
of the electronics, but will toss the rest unless someone would like it.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu










From daemon Thu Jun 08 18:12:46 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Thu, 08 Jun 2000 09:47:26 -0500
Subject: Re: JEOL 2010 LaB6 Question

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hello Kim, Jordi & interested parties,
We have a filament ramp circuit on our LaB6 2010 that controls the filament
current. It is an accessory offered by Jeol. Jeol has set it to take about 8
minutes to bring the filament to max current. The max. current is still
controlled by the (preset) filament current knob on L1. Once the filament is
hot, when we change samples or other wise have the filament current off for
{5mi. we use the "quick timer" feature of this circuit which brings the filament
up in ~90 seconds.
The max. filament current is set to run just under saturation (so you can
just barely see the X when the beam is converged). The bias control is used to
set beam current to 10 uA. The setting changes with KV & filament wear. The
initial bias setting is a function of the physical position of the filament
relative to the Wehnelt cap. It changes every time the filament or cap are
serviced. As the tip wears the difference in bias settings say between 100 &
200 KV increases & both move up.
We are a multiple user facility. Our filament sees a of cycles & we always
have novice users. Filament life times are 400-1000 hours. This tends to track
with the novice user density.
As far as the drift Jordi mentioned. This is a non issue here & may be in
the filament design. We use the Gimble Phillips 60-06. I do a lot of carbon
work & can pretty much nail 3.4A when I get a beam. Yes the images are sharper
after we have been running a bit.

Bruce Brinson
Rice U.

usual disclaimer... no financial interest in companies mentioned.

Marti, Jordi wrote:

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}
} Kim:
}
} We have a 2010 and our procedure is to bring the filament dial (labeled
} off-10) to position #3. Then we go for coffee. After 20 min. or so we raise
} it to # 4, after a couple of minutes to #5 etc. until we get to the stop (
} in our case we have it at about 7 1/2). Even at this point we have filament
} drift for quite some time, so , we are not ready to take pictures for
} another 1/2 hr or so. By the way, we have the bias settings at 7 and 7 which
} is high. I would be interested in knowing what your settings are.
}
} I hope this helps
}
} Jordi Marti
}



From daemon Thu Jun 08 18:12:59 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 8 Jun 2000 11:13:17 -0500
Subject: Stereometry by computer

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A user of our facility is interested in making height measurements
from specimens viewed in the SEM. I am aware of the conventional way
of doing this: stereo pairs and optical viewer (with stereometer
parallax corrections). Is there a more modern (computerized) way of
doing this, say with anaglyphs?

Thank you.

John B.


####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Thu Jun 08 18:12:59 2000



From: Al :      alchazz-at-ix.netcom.com
Date: Thu, 8 Jun 2000 07:59:26 -0500
Subject: Re: SEM: agency wants SEM images?

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I work for an analytical lab, Analytical Answers, Inc., located in Woburn, MA.

We charge $200/hr for SEM imaging, high resolution SEM imaging is more. The
customers are free to do what they want with the SEM images.

They paid for them, they own them!

Al Jaszek




-------------------------------------
*Causa latet, eventus est notissimus*
-------------------------------------




From daemon Thu Jun 08 18:13:06 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 8 Jun 2000 13:40:05 EDT
Subject: Re: Stereometry by computer

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In a message dated 6/8/00 12:38:48 PM, bozzola-at-siu.edu writes:

} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?


There was a thread recently on measurement of stereo pairs by
computer-matching of points. Several software packages (one of the is Fovea
Pro - http://members.aol.com/FoveaPro) were mentioned as having this
capability. Whether or not the two images are put together as an anaglyph is
unimportant.



From daemon Thu Jun 08 18:13:09 2000



From: David Bentley :      dlb-at-u.arizona.edu
Date: Thu, 08 Jun 2000 11:39:28 -0700
Subject: Re: Help? Cleaning Optical Lenses

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Just a couple of additional comments to Barbara's procedure.

A Zeiss axiom: If the lens is not dirty, then cleaning it will never
improve it, it only risks damaging it. Do the least amount possible to
return the surface to clean. If the surface is only dusty, & puffing it
removes the problem, stop there. I look at the lens being cleaned, using
the ocular turned upside down as a loupe, at each step of the process.
When it's clean, you're done. Usually only external surfaces are
contaminated and need frequent or extensive cleaning. Internal lenses, a
dusting often suffices.

In dealing with a dissecting microscope with a zoom magnification
capability as opposed to one with click stops or a fixed magnification, be
very careful about disturbing the positional settings of the internal
lenses. Any changes to these settings will alter magnification for the
each of the two eyes and make an image that can't be justified in the
brain. This will require a service engineer to remedy. For heavily filmed
lenses, use short or broken cotton tipped applicator and get in the best
you can. Varying the zoom control will move the lenses up and down and
maybe give you enough room to work.

As Barbara wrote, removing loose dust is essential to preserving lens
quality. Lenses are coated with various coatings and these are easily
damaged. Puffers from camera stores are good. The red bulb ear syringe
for babies is excellent and usually readily available. I do use dusters
and compressed air although both are frowned upon, by some, as possibly
damaging lens coatings. If you use a duster, don't shake or tip it while
dusting the lens. This will expel liquid from the can and contaminate the
lens worse.

A good lens cleaner that is easy to get is Sparkle Glass Cleaner available
from Ace hardware, and grocery stores (No financial interests). Never use
Windex. It contains oils that will coat the lens.

As much as possible use the cotton tipped applicators rather than lens
tissues. Unless you wear latex or polyethylene gloves, finger oils will
get onto the tissue and be transferred to the lens. Another reason to shy
away from the tissue is the tendency to scrub the lens. I agree with
Barbara, NEVER, NEVER Kimwipes, I've been told from many sources they
contain many silica strands, and will scratch the delicate optical
coatings. On expensive lenses, make a single pass, using minimal pressure
straight across the lens, with the applicator, rolling the stick between
your fingers to present a fresh cotton surface to the lens and picking up
and getting the dust away from the lens. Alternate between wet (either
with lens cleaner or condensed breath (open mouth, no spit please) and dry
cotton. (Yes, it is often necessary to use a swirling motion on oculars
because of the amount of oil contamination from the eye lashes.)

It is best not to try to clean filters, there are a few recommended
procedures but all potentially damage the very precise and thin coatings of
the filter compromising its performance. (If anyone disagrees or has a
favorite procedure for filter cleaning, contact me off list, I'd like to
hear about it.)

Use of solvents or disassembling objectives or multiple lens stacks is
best left to the service engineer. Lenses use a variety of air and cement
interfaces to achieve resolution and aberration correction. Altering these
by dissolving the cement will degrade the lens. Getting the small lenses
back in exactly the same position is also very, very difficult and not for
the faint hearted.

There is a microscope repair workshop in the initial planning stages for
the Long Beach MSA meeting next year. It will target K-12 school teachers,
but will hopefully address many levels. If members of the list will be at
the meeting and are interested in attending this workshop to: 1. learn
basic repairs for their microscopes. 2. get ideas for their outreach
program. or 3. assist with putting on the workshop. Please e-mail me
telling me of your interest or asking for more information.

If you have any questions feel free to contact me off line.



From daemon Thu Jun 08 18:13:10 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Thu, 8 Jun 2000 12:11:37 -0700
Subject: RE: plant vesicles

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Jim is absolutely right about LR white. But instead of increasing the GA I
will probably increase paraformaldehyde conc. to 4%.

Good luck,

Soumitra


} } Hi,
} }
} } I'm trying to do immunogold studies on a membrane protein in a preparation
} } of plant vesicles (from minus 80oC storage). I did an Epon embedding with
} } standard procedure (glut and osmium) to check the ultrastructure and got
} } decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
} } paraformaldehyde, without osmium) looks like a disaster, with granular
} } material, vague membranish-like structures and whorls of membranes. Does
} } anyone have any tips for LR White preps of vesicles, or any other ideas
} } for embedding (resins, fixing, dehydration, embedding) to favour the
} } immunogold process.
} }
} } Thanks,
} }
} } Mark
} }
} }
} }
} }
} }
} }
} } ********************************************
} } Mark West,
} } Unidad de Microscopia Electronica,
} } (Electron Microscopy Unit)
} } Instituto de Fisiologia Celular,
} } Universidad Nacional Autonoma de Mexico,
} } 04510 Mexico D.F.
} }
} } tel (unidad/lab) *(525) 622 5610*
} } (casa/home) (525) 619 3020
} } Fax (525) 616 2282
} } ********************************************
} }




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Thu Jun 08 18:13:11 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Thu, 8 Jun 2000 15:21:15 -0400 (EDT)
Subject: Re: Help? Cleaning Optical Lenses

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On Thu, 8 Jun 2000, David Bentley wrote:

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} -----------------------------------------------------------------------.
text deleted
}
} It is best not to try to clean filters, there are a few recommended
} procedures but all potentially damage the very precise and thin coatings of
} the filter compromising its performance. (If anyone disagrees or has a
} favorite procedure for filter cleaning, contact me off list, I'd like to
} hear about it.)
}
Please respond on-line! I'm sure that I'm not the only other person who
would be interested in this!

Tamara Howard
CSHL




From daemon Thu Jun 08 18:13:17 2000



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-Em.agr.ca
Date: Thu, 08 Jun 2000 16:44:55 -0400
Subject: Re: plant vesicles

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Try treat the glut- & Os-fixed sections you have with sodium metaperiodate before doing immunolabeling. It may work.

Reference:
M. Bendayan 1989. Protein A-gold and Protein G-gold postembedding immunoelectron microscopy. In Colloidal Gold: Principles, Methods and Applications Vol.1 Academic Press.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } Mark West {mwest-at-ifcsun1.ifisiol.unam.mx} 06/07 11:50 AM } } }
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Hi,

I'm trying to do immunogold studies on a membrane protein in a preparation
of plant vesicles (from minus 80ŒC storage). I did an Epon embedding with
standard procedure (glut and osmium) to check the ultrastructure and got
decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
paraformaldehyde, without osmium) looks like a disaster, with granular
material, vague membranish-like structures and whorls of membranes. Does
anyone have any tips for LR White preps of vesicles, or any other ideas
for embedding (resins, fixing, dehydration, embedding) to favour the
immunogold process.

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************





From daemon Thu Jun 08 18:13:18 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 9 Jun 2000 08:48:46 GMT+1200
Subject: Re: JEOL 840 SEM Vacuum Problem

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Sorry, I don't have any useful suggestions, and for that reason, I'd
rather see responses posted to the list, if possible, as this gives
me a wonderful and otherwise unavailable opportunity to learn from
the experience of others.

cheers

rtch







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} -----------------------------------------------------------------------.
}
}
} We have JEOL 840 SEM that has develop a leak at the specimen chamber door
} interface and will not hold a vacuum. We have changed the o-ring around the
} door, look for nicks, scratches any other damage around the door interface, none
} found. We have also check the roughing valve (V4, LV3 and the pressure relief
} valve) for leaks. Pressure holds in the gun area. The large door is only opened
} when we have to replace or add a new attachment to the chamber and thats about
} once a year. We have a vacuum specimen exchange port to enter and retrieve
} samples. We have also check that seal and it also checks out OK.
} We have had 2 JEOL engineers take a look at the system and at this point no luck
} finding the cause or solution. You can respond to me off-line at
} bruce.arey-at-pnl.gov
} Thanks
}
} Bruce W. Arey
}
} PNNL-Battelle
} Associate Scientist
} Microstructural Characterization
} bruce.arey-at-pnl.gov
} 509-376-3363
} fax 509-376-6308
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Jun 08 18:13:19 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Thu, 8 Jun 2000 15:59:48 -0500
Subject: RE: plant vesicles

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Mark, you didn't say how you post-stained after you immunolabeled. This step
can make all the difference in the world. For LRW, I prefer 3-4 secs in
saturated UA in 50% etoh, wash, followed by about 15secs in lead citrate. I
find a major difference (inferior) when I use aqueous UA. Also, going too long
in any of the solutions will give poor results.

} ===== Original Message From Mark West {mwest-at-ifcsun1.ifisiol.unam.mx} =====
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Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Thu Jun 08 18:13:29 2000



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Thu, 8 Jun 2000 15:43:51 -0700 (PDT)
Subject: Re: Stereometry by computer

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John - I routinely make height measurements of 5 - 10 um interplanetary
dust particles using the objective focus knob on our JEOL 1200EX in STEM
mode. The procedure is simply to focus on the top of the particle and
note the number of steps (or count the number of clicks of the knob)
required when refocussing on the substrate next to the particle. By
calibrating the objective focus step size with a known standard, you can
routinely measure particle heights. In principle, a similar procedure
should be possible on an SEM, depending on how the objective focus is
configured. I don't think you can expect high accuracy with this method,
however, so it may not be applicable to your needs.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seattle, WA 98195
joswiak-at-astro.washington.edu

On Thu, 8 Jun 2000, John J. Bozzola wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?
}
} Thank you.
}
} John B.
}
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}
}



From daemon Thu Jun 08 18:13:29 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Thu, 08 Jun 2000 15:59:22 -0700
Subject: RE: Cleaning optical lenses and filters.

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Tamara et al : One superior method for cleaning sensitive optical components such as filters and front surfaced telescope mirrors is as follows: 1) gently puff of extraneous dust. 2) gently float the surface with deionized water. 3) if necessary to remove dirt film use a q-tip soaked in a non-ionic surfactant like a 0.01% solution of triton-x 100 (IMPORTANT DO NOT RUB ON THE SURFACE WITH FORCE BUT SIMPLY LET THE WEIGHT OF THE WET Q-TIP(S) BE THE ONLY "DOWNWARD PRESSURE" ON THE SURFACE OF THE GLASS. Such gently techniques such as these should help mitigate damage.



From daemon Thu Jun 08 18:13:30 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Thu, 08 Jun 2000 16:03:20 -0700
Subject: RE: Cleaning optical lenses and filters.

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Tamara et al : (excuse mispelling in first copy sent) One superior method for cleaning sensitive optical components such as filters and front surfaced telescope mirrors is as follows: 1) gently puff off extraneous dust. 2) gently float the surface with deionized water. 3) if necessary to remove dirt film use a q-tip soaked in a non-ionic surfactant like a 0.01% solution of triton-x 100 (IMPORTANT DO NOT RUB ON THE SURFACE WITH FORCE BUT SIMPLY LET THE WEIGHT OF THE WET Q-TIP(S) BE THE ONLY "DOWNWARD PRESSURE" ON THE SURFACE OF THE GLASS. Such gently techniques such as these should help mitigate damage.



From daemon Thu Jun 08 18:13:31 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 8 Jun 2000 16:02:44 -0700
Subject: Re: LRWhite flat embedding

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} I remember a message some time ago, I think, suggesting a way to flat
} embed in silicon molds using LRWhite resin. How the top was sealed
} escapes me. Could anyone with a better filing system and/or memory
} please send me a message or a reference on this.

Peter Bond {P.Bond-at-plymouth.ac.uk}

} Pete -

If you have a silicon mold that isn't permeable to LR White, I'd like to
know who makes it! There is a teflon flat mold available from Ted Pella
that works well with LR White; it's their own product. It's sealed with
Thermanox coverslips, so you might try them with your mold.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu Jun 08 19:33:34 2000



From: Dopeyee-at-aol.com
Date: Thu, 8 Jun 2000 20:28:48 EDT
Subject: LEAK IN JEOL

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GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING

I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP

THEY HAVE TO FIX IT!!!!



From daemon Fri Jun 09 06:11:49 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 08 Jun 2000 22:11:20 -0500
Subject: LR White flat embedding

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Peter Bond wrote:
==============================================================
I remember a message some time ago, I think, suggesting a way to flat embed
in silicon molds using LRWhite resin. How the top was sealed escapes me.
Could anyone with a better filing system and/or memory please send me a
message or a reference on this.
==============================================================
This might have been an old posting of mine. Since UV transparency is
usually required, we are talking about clear UV transparent silicone
embedding molds and we recommend a slight "overfilling" of the cavities.
Then when all cavities are filled, take another mold, just like the first
one, and place it on top, bottom side down, cavity side up. The capillary
action will result in a sealing out of any oxygen.

When the UV curing is complete, the top mold can be easily separated and
since the cavity side is still unused, no wear and tear has been put on it
in terms of taking away any of its lifetime.

Information about these transparent-to-UV embedding molds, and their use,
can be found on the SPI website given below.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Jun 09 06:12:09 2000



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Fri, 9 Jun 2000 09:19:55 +0200
Subject: A hole in the EDS spectrum

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Dear colleagues,
We have a problem with spectra recording in our EDAX DX4 system
mounted on Philips CM12/STEM. There is a hole in the spectrum between
0.5keV and 3.5keV.
With service engineer, we have borrowed all the boards in electronics
and exchange our boards with the borrowed ones. The hole in the
spectrum remains. We have also dismounted detector from the EM column
and the Be window was checked for contamination. The window was clean
and intact.
Please, can anybody give us any hints how to solve our problem? Many
thanks in advance for any comment.
Oldrich Benada

Our system specification:
Philips CM12/STEM
EDAX DX4 system with 184 preamp. based on win3.11





+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm



From daemon Fri Jun 09 06:12:09 2000



From: Ulrike Zeile :      ulrike.zeile-at-bal-tec.com
Date: Fri, 9 Jun 2000 09:44:26 +0200
Subject: AW: service

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Hello Mr.Patel,

Balzers Union renamed to BAL-TEC AG in 1992. But the product
line was maintained and new developments are carried out.
For additional information have a look at our website www.bal-tec.com.
There are several coating systems and freeze fracture systems of this older
types in use.

Our representative in USA / Canada:

Techotrade International
7 Perimeter Road
Manchester, NH 03103-3343
T: +1 603 622-5011

Our representative will contact you directly to assist with
further information.

-----UrsprŸngliche Nachricht-----
Von: Rajesh Patel [mailto:rpatel-at-UMDNJ.EDU]
Gesendet: Mittwoch, 31. Mai 2000 19:42
An: microscopy-at-sparc5.microscopy.com
Betreff: service


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We need service on our Balzer 500 freeze fractrure unit,
and was wondering who services them. Anyone we can contact?

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu



From daemon Fri Jun 09 06:12:15 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Fri, 09 Jun 2000 11:53:08 +0200
Subject: Carbonate standards - bizarre behaviour

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G'day Folks,
I acquired some carbonate standards a while ago from the Smithsonian
Insitute. Namely, Calcite, Siderite, Dolomite and Strontianite.
Interestingly, when admiring them with our ED system, there appeared an
anomalous peak around about where B is supposed to be. This is not a
detector artifact as it is specific only to these materials. I just
wondered if anyone else had noticed the same thing, or whether anyone
has any clues on why this should be occuring. The programme suggests
that there is about 50 wt % B in these things which seems unbelievable
considering the compositions supplied by Washington. Ideas?
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Fri Jun 09 07:42:10 2000



From: HARRISm-at-esm-semi.co.uk
Date: Fri, 09 Jun 2000 10:12 +0000 (GMT)
Subject: RE: JEOL840 SEM VACUUM PROBLEMS

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It may not help but .....

With regard to the vacuum problems encountered by Bruce , without
being familiar with the instrument we encountered a ' similar '
problem and were misled into thinking a chamber door leak existed by
an over zealous leak detection unit .
The problem on our sem actually turned out to be a hairline crack in a
metal bellows attached to a valve that appeared OK . The vaccum would
not exceed a certain level in pumpdown in its final stages .

I believe these bellows were often a source of problems on older
instruments .

M.HARRIS harrism-at-esm-semi.co.uk
ESM LTD
South Wales , U.K .



From daemon Fri Jun 09 08:52:00 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 9 Jun 2000 08:51:11 -0400
Subject: RMC microtomes

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Hi All,
I'm posting this for a friend who is not on-line.

Does anyone out there have a used RMC MT-7000 for sale? My friend just
moved to a lab that had an MT-5000 which she was told was operational. A
major exaggeration. She is an RMC loyalist, and desparately wants another,
but has a limited budget.

Please contact: Linda Burg Friedman at Columbia P & S at (212)305-9047

Thanks,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 09 08:52:00 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 9 Jun 2000 14:34:56 +0100
Subject: Re: JEOL 840 SEM Vacuum Problem

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Hi

The specimen chamber is a vast area with many potential leak sources.

The door is the most likely if it is being opened on a regular basis,
however do not forget that the stage drives pass through the door and they
are being used all of the time.

If you are sure the door "O" ring is OK check the stage drive feed throughs
as they may be the source of your problem?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Fri Jun 09 09:01:58 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 9 Jun 2000 09:05:24 -0400
Subject: Re: TEM: Set-up, operating and facilities costs

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Rick,
YOu don't mention make or modle of TEM, but i can tell you that my service
contract (2 preventive maintenance call, unlimited service calls, parts,
labor) costs around $15,500/year.
You will need a water supply for cooling and electrical work to bring in a
dedicated line. You will need a dakrroom with running water and a temp.
control valve to process the film. If you wish to make photographic prints
of your negatives, you will also need a point source enlarger (Durst was
the best, but they are hared to find, Omega used to make one, too) and
either pans (slow and painful) or a rapid processor.
Budgeting for supplies is a tough call...it depends on your usage.
If you find someone with an EM background to run it, its should't take them
long to learn the individual instrument. Training someone from ground zero
could take months to get dthe person on his/her way.
Ancillary euqipment: it depends on what you will be doing, biological or
materials, embedding/sectioning or particulates, negataive staining or
metal shadowing.

A really good source to check is Audrey Glauert's Practical Methods in
Electron Microscopy Vol. 4: Design of the Electron Microscope Laboratory,
by Ronald H. Alderson, North-Holland publishers, 1975.

If you wish to contact me off-line, I can go over my lab's operating budget
with you. I run a basic EM Core Facility here at (what used to be called)
Cornell Medical College.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 09 09:11:57 2000



From: Arey, Bruce W :      bruce.arey-at-pnl.gov
Date: Fri, 09 Jun 2000 07:05:10 -0700
Subject: RE: LEAK IN JEOL

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JEOL has been very helpful and they are somewhat limited on what they can do to
this instrument. The 840 has radioactive particles inside the chamber so we have
to be careful each time we open the system up plus there hands on is limited to
just oversight. I am in the process of trying to find a leak detector on site
that can be used on radioactive system. But we also cannot pump down the chamber
enough to use a leak detector. So we are in the process of making a plate that
will fit over the door. We have taken off all our accessories off the chamber
(EDS, OIM, BSE) and have pressurized the system and have found some evidence of
a leak on the left hand side on the door, we have changed the o-ring and still
the seal leaks on this side of the door. We have polished the door seal to make
sure there is no major scratches or marks. We are pretty confident that specimen
exchange port is OK we can pump this down and we see no signs of a leak. Why are
we focusing our attention on the large o-ring and chamber door. We can clean
the o-ring with alcohol (methanol or ethanol) and we can rough pump the chamber
out and put the chamber on the high vac system but after a period of time the
alcohol dries out and the system shuts down too a poor vacuum (overnight). Then
we try to rough pump the chamber and we cannot go more than 20-30 mamps
difference on the pirani gauge. We have done this several times with the same
results. We are going to try to pressurize the system with He and try He sniffer
to help us maybe pin point the leak. Thanks to all who have responded with
suggestion on finding the leak and if we are successful in finding the leak I
will post the finding on the server. Again JEOL has been very responsive in
trying to find the leak and they are somewhat limited on this instrument do to
its environment. Any other suggestion are welcomed.


Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308


----------
From: "Dopeyee-at-aol.com"-at-sparc5.microscopy.com
Sent: Thursday, June 8, 2000 4:28 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: LEAK IN JEOL

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GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING

I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP

THEY HAVE TO FIX IT!!!!



From daemon Fri Jun 09 09:11:57 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 9 Jun 2000 09:05:55 -0500
Subject: RE: JEOL 840 SEM Vacuum Problem

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I recall many years ago we had a similar vacuum leak on our 840A that was
related to the specimen exchange port mechanism. The moving parts in the
sliding door mechanism had become slightly miss-aligned. We ended up
disassembling that mechanism, installing new o-rings and lubricating with
Apiezon L. Problem gone.
Hope your leak is that easy!
Good Luck
Brad Huggins
BP Amoco, Naperville

} ----------
} From: Arey, Bruce W[SMTP:bruce.arey-at-pnl.gov]
} Sent: Thursday, June 08, 2000 9:24 AM
} To: 'Microscopy-at-msa.microscopy.com'
} Subject: JEOL 840 SEM Vacuum Problem
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} We have JEOL 840 SEM that has develop a leak at the specimen chamber door
} interface and will not hold a vacuum. We have changed the o-ring around
} the
} door, look for nicks, scratches any other damage around the door
} interface, none
} found. We have also check the roughing valve (V4, LV3 and the pressure
} relief
} valve) for leaks. Pressure holds in the gun area. The large door is only
} opened
} when we have to replace or add a new attachment to the chamber and thats
} about
} once a year. We have a vacuum specimen exchange port to enter and retrieve
} samples. We have also check that seal and it also checks out OK.
} We have had 2 JEOL engineers take a look at the system and at this point
} no luck
} finding the cause or solution. You can respond to me off-line at
} bruce.arey-at-pnl.gov
} Thanks
}
} Bruce W. Arey
}
} PNNL-Battelle
} Associate Scientist
} Microstructural Characterization
} bruce.arey-at-pnl.gov
} 509-376-3363
} fax 509-376-6308
}
}
}



From daemon Fri Jun 09 17:35:54 2000



From: milesd-at-us.ibm.com
Date: Fri, 9 Jun 2000 10:24:19 -0400
Subject: RE: Cleaning optical lenses and filters.

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I have always understood that deionized water would etch metal,
that being the reason for PVC pipe being used to deliver it. If this
is true, wouldn't that damage the first surface mirrors?

Darrell



From daemon Fri Jun 09 17:35:55 2000



From: ComCryLab1-at-aol.com
Date: Fri, 9 Jun 2000 10:31:59 EDT
Subject: LM: alignment

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David,
We have a B&L StereoZoom (0.7X - 3X) microscope which we
intend to donate to the local school system
(Computer VoTech Dept) for use in examining the circuit patterns
on silicon wafers that I have donated to them.

My problem is that the microscope is not functioning properly.
The two optical paths are not synchronized during zooming and
therefore the image tends to rotate or it falls out of focus. We have
tried to repair it in-house but we were unsuccessful. Knowing that
that we would create additional problems, we did not disturb the
lens or prism sub-assembly.

Is there a set of maintenance instructions or a tech manual available
to help us align this scope?

Thanks
Mike Urbanik
Commercial Crystal Labs
Naples, FL
www.crystalguru.com



From daemon Fri Jun 09 17:35:57 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 9 Jun 2000 08:46:11 -0600
Subject: Re: Stereometry by computer

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John,

you may want to try the method below if your samples show very large
structures. One of the nice features of an SEM is it's large depth of
focus, unfortunately in many cases this prevents you from using the
technique mentioned below. You can use a stereo technique to calculate a
surface profile (see for example the stereo module on our web site or
other stereo applications). Typical results from stereo images have a
height resolution of about 1/10 the of the lateral resolution (in other
words: if your lateral resolution is 1 micron between pixels, the height
resolution will be on the order of 10 microns). This can be improved by
sub-pixel interpolation but gives you an order of magnitude for the
resolution.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: David Joswiak [mailto:joswiak-at-orca.astro.washington.edu]
Sent: Thursday, June 08, 2000 4:44 PM
To: John J. Bozzola
Cc: Microscopy-at-sparc5.microscopy.com


John - I routinely make height measurements of 5 - 10 um interplanetary
dust particles using the objective focus knob on our JEOL 1200EX in STEM
mode. The procedure is simply to focus on the top of the particle and
note the number of steps (or count the number of clicks of the knob)
required when refocussing on the substrate next to the particle. By
calibrating the objective focus step size with a known standard, you can
routinely measure particle heights. In principle, a similar procedure
should be possible on an SEM, depending on how the objective focus is
configured. I don't think you can expect high accuracy with this
method,
however, so it may not be applicable to your needs.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seattle, WA 98195
joswiak-at-astro.washington.edu

On Thu, 8 Jun 2000, John J. Bozzola wrote:

}
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}
}
} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?
}
} Thank you.
}
} John B.
}
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}
}



From daemon Fri Jun 09 17:36:06 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Fri, 09 Jun 2000 12:29:09 -0500
Subject: LR White flat embedding

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This may sound like "bucket science" but we've used the disposable aluminum
weighing dishes to flat embed material. If you fill the bottom tray about
1/2 full and set another tray inside it, press gently until a little LR
white oozes up the sides, what remains in the middle will be protected from
the air and should polymerize nicely. (Maybe we've just been lucky!)
good luck!

Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Fri Jun 09 17:36:07 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Fri, 09 Jun 2000 13:53:37 -0400
Subject: Re: Carbonate standards - bizarre behaviour

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Malcolm:

Some Chlorine-L lines, as well as Sr lines have nearly identical
energy/wavelength as B Ka. I don't remember the chemistry of these
standards offhand, but I know some of them were Sr-bearing and some may also
have Cl. These are likely the peaks you are seeing at the low end.

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610


Dr Malcolm Roberts wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} G'day Folks,
} I acquired some carbonate standards a while ago from the Smithsonian
} Insitute. Namely, Calcite, Siderite, Dolomite and Strontianite.
} Interestingly, when admiring them with our ED system, there appeared an
} anomalous peak around about where B is supposed to be. This is not a
} detector artifact as it is specific only to these materials. I just
} wondered if anyone else had noticed the same thing, or whether anyone
} has any clues on why this should be occuring. The programme suggests
} that there is about 50 wt % B in these things which seems unbelievable
} considering the compositions supplied by Washington. Ideas?
} Cheers,
} Malc.
}
} --
} Dr MP Roberts Phone: [+27](0)46 603 8313
} Dept of Geology Fax: [+27](0)46 622 9715
} Rhodes University Cell: 083 4060 262 (usually off)
} 6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
} SOUTH AFRICA







From daemon Fri Jun 09 17:36:10 2000



From: bobrob-at-uswest.net
Date: Fri, 09 Jun 2000 11:49:20 -0700
Subject: SEM: JEOL 840 Vacuum Problem

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If the instrument has been physically moved recently, this can sometimes

hasten a crack in these stainless steel "flex" vacuum lines. I have
found
these to develop mostly at the end of the tube where it has been flared
for
the fitting connector. Check the roughing lines first.

Happy hunting...

Bob Roberts
EM Lab Services, Inc
2409 S. rural Rd Suite C
Tempe, Arizona 85282
480.967.3946



From daemon Fri Jun 09 17:36:10 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 9 Jun 2000 12:18:33 -0500
Subject: RE: A hole in the EDS spectrum

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} Oldrich
Need a little more information.
Do you actually have spectral information in the spectrum at energies { 0.5,
or is there possibly, only noise in this low energy range? If the signal
present in your spectra at these very lowest energies is just noise-like
signal. Then it is possible that you have a severe alignment problem with
the EDS detector/specimen geometry. A combination of high noise (due to
vibration or other sources) and poor line of sight with the detector window
(resulting in detection of only the higher energy x-rays ) could give you
this "hole in the EDS spectrum" effect. A miss-aligned detector, and/or a
detector making contact with the internal parts of the microscope might
create this situation. Does the information in the low energy region
correlate with the specimen composition? Does the high energy signal
correlate with the specimen composition?

} ----------
} From: Oldrich Benada[SMTP:benada-at-biomed.cas.cz]
} Sent: Friday, June 09, 2000 2:19 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: A hole in the EDS spectrum
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Dear colleagues,
} We have a problem with spectra recording in our EDAX DX4 system
} mounted on Philips CM12/STEM. There is a hole in the spectrum between
} 0.5keV and 3.5keV.
} With service engineer, we have borrowed all the boards in electronics
} and exchange our boards with the borrowed ones. The hole in the
} spectrum remains. We have also dismounted detector from the EM column
} and the Be window was checked for contamination. The window was clean
} and intact.
} Please, can anybody give us any hints how to solve our problem? Many
} thanks in advance for any comment.
}
} Oldrich Benada
}
} Our system specification:
} Philips CM12/STEM
} EDAX DX4 system with 184 preamp. based on win3.11
}
}
}
}
}
} +-----------------------------------+
} Oldrich Benada
} Acad. Sci. CR
} Institute of Microbiology
} Laboratory of electron microscopy
} Videnska 1083
} CZ - 142 20 Prague 4 - Krc
} Czech Republic
} +------------------------------------+
} Phone: +420-2-4752399
} Fax: +420-2-4715743
} WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm
}



From daemon Fri Jun 09 17:36:14 2000



From: hard-at-acsu.buffalo.edu
Date: Fri, 09 Jun 2000 16:18:05 -0500
Subject: Course Announcement I

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Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 11 - October 19, 2000

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2150 (Includes room and board, text, handouts, supplies)

Application Deadline: August 1, 2000

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided
by major optical and electronics companies. Instruction will be provided by
experienced staff from universities and industry.

Students are encouraged to bring their own biological (primary
cultures, cell lines, etc.) and material specimens and to discuss individual

research problems with the faculty.



From daemon Fri Jun 09 17:50:39 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 9 Jun 2000 17:38:24 -0500
Subject: University of Oxford: Job Vacancies

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University of Oxford: Job Vacancies



DEPARTMENT OF MATERIALS



Postdoctoral Research Assistant - Electron Microscopy of Crystalline
Materials



Salary £16,286 - £24,479 p.a.

A three-year position is available in a research group being developed by
Professor David Cockayne FRS for the study of a range of materials using
electron diffraction, electron microscopy (EM) and modelling techniques.
Quantitative microscopy and materials modelling will refine structural
models of technologically important materials. Extensive expertise in EM,
diffraction, the preparation of crystalline materials for microscopy, and
strong computing skills, are essential. Expertise in microscopy of
semiconductors including quantum dots and computational techniques for
image simulation would be an advantage. The Department has outstanding EM
and modelling facilities. Please quote ref. DJ00/12.

Applications including a curriculum vitae, list of publications and the
names and addresses of three referees should be sent to The Administrator,
Department of Materials, University of Oxford, Parks Road, Oxford, OX1
3PH, from whom further particulars are available. The closing date for
applications is 14 June 2000.









From daemon Sat Jun 10 07:40:26 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 10 Jun 2000 13:56:13 +1000
Subject: RE: LEAK IN JEOL

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I have found the use of clear silcone sealant (as used for windows etc) quite
effective to temporally fix or help to isolate large and medium vacuum leaks.
In many instance it can be applied externally over various fittings. Screw
holes are best covered first with a little tape, to facilitate the later the
removal of the dry silicone. Silicone outgases a fair bit for some hours, so
only major leaks can be determined immediately after applying the silicone.
The method seems crude but is effective to eliminate numerous fittings as the
source of a leak. I once operated a TEM for several months with a split
stainless bellow, patched with a smear of silicone sealant.
I don't suggest the use of that sealant on a permanent basis or in ion gutter
pumped parts of a column.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 10, 2000 12:05 AM, Arey, Bruce W [SMTP:bruce.arey-at-pnl.gov]
wrote:
}
}
} JEOL has been very helpful and they are somewhat limited on what they can do
} to
} this instrument. The 840 has radioactive particles inside the chamber so we
} have
} to be careful each time we open the system up plus there hands on is limited
} to
} just oversight. I am in the process of trying to find a leak detector on site
} that can be used on radioactive system. But we also cannot pump down the
} chamber
} enough to use a leak detector. So we are in the process of making a plate
} that
} will fit over the door. We have taken off all our accessories off the chamber
} (EDS, OIM, BSE) and have pressurized the system and have found some evidence
} of
} a leak on the left hand side on the door, we have changed the o-ring and
} still
} the seal leaks on this side of the door. We have polished the door seal to
} make
} sure there is no major scratches or marks. We are pretty confident that
} specimen
} exchange port is OK we can pump this down and we see no signs of a leak. Why
} are
} we focusing our attention on the large o-ring and chamber door. We can clean
} the o-ring with alcohol (methanol or ethanol) and we can rough pump the
} chamber
} out and put the chamber on the high vac system but after a period of time the
} alcohol dries out and the system shuts down too a poor vacuum (overnight).
} Then
} we try to rough pump the chamber and we cannot go more than 20-30 mamps
} difference on the pirani gauge. We have done this several times with the same
} results. We are going to try to pressurize the system with He and try He
} sniffer
} to help us maybe pin point the leak. Thanks to all who have responded with
} suggestion on finding the leak and if we are successful in finding the leak I
} will post the finding on the server. Again JEOL has been very responsive in
} trying to find the leak and they are somewhat limited on this instrument do
} to
} its environment. Any other suggestion are welcomed.
}
}
} Bruce W. Arey
}
} PNNL-Battelle
} Associate Scientist
} Microstructural Characterization
} bruce.arey-at-pnl.gov
} 509-376-3363
} fax 509-376-6308
}
}
} ----------
} From: "Dopeyee-at-aol.com"-at-sparc5.microscopy.com
} Sent: Thursday, June 8, 2000 4:28 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LEAK IN JEOL
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING
}
} I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP
}
} THEY HAVE TO FIX IT!!!!
}



From daemon Sat Jun 10 07:40:26 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 10 Jun 2000 18:59:21 +1000
Subject: RE: Cleaning optical lenses and filters.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hmmm, and "I didn't even know that", but believed that deionised water had
metal ions removed from it and so in that respect its purer than 2x glass
distilled water. Then I was taught and believed! that metal pipes would
re-introduce metal ion back into the water! Being deionised the water has no
buffering capacity and therefore is neither acid nor alkaline, they told me and
I believed.
Education is expensive; must ask for my money back.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 10, 2000 12:24 AM, "milesd-at-us.ibm.com"-at-sparc5.microscopy.com
[SMTP:"milesd-at-us.ibm.com"-at-sparc5.microscopy.com] wrote:
}
}
} I have always understood that deionized water would etch metal,
} that being the reason for PVC pipe being used to deliver it. If this
} is true, wouldn't that damage the first surface mirrors?
}
} Darrell
}



From daemon Sat Jun 10 14:45:31 2000



From: milesd-at-us.ibm.com
Date: Sat, 10 Jun 2000 15:24:26 -0400
Subject: RE: Cleaning optical lenses and filters.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ahhh, but I have now been educated! Re-contamination of the
painstakingly purified water is the concern, and there is no
threat to the durability of the pipes. I had been mislead.

Darrell



From daemon Mon Jun 12 08:26:19 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Mon, 12 Jun 2000 07:57:51 +0200
Subject: RE: LEAK IN JEOL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
Vacuum leaks, what a pleasure!
Our tried and tested methods include using Petroleum Ether or Ethanol and
then Bostick Prestic or Blue tac as it is known in Australia.
Spraying alcohol around the suspected areas should show up the leak.
If you ant to try and stop a leak use the Prestic. Its that stuff you buy at
the stationary shop that is used to stick posters and pictures to a wall.
That is pliable, removable and really handy at sealing off a few suspect
areas.
We are currently working on a JEOL840 here with a leak. After many hours we
found that it was the gauge head that was leaking.

Good luck
Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
www.anaspec.co.za

ICEM 15 will be in Durban,
South Africa, 2002.
www.icem15.com

-----Original Message-----
} From: Arey, Bruce W [mailto:bruce.arey-at-pnl.gov]
Sent: Friday, June 09, 2000 4:05 PM
To: 'microscopy-at-msa.microscopy.com'


JEOL has been very helpful and they are somewhat limited on what they can do
to
this instrument. The 840 has radioactive particles inside the chamber so we
have
to be careful each time we open the system up plus there hands on is limited
to
just oversight. I am in the process of trying to find a leak detector on
site
that can be used on radioactive system. But we also cannot pump down the
chamber
enough to use a leak detector. So we are in the process of making a plate
that
will fit over the door. We have taken off all our accessories off the
chamber
(EDS, OIM, BSE) and have pressurized the system and have found some evidence
of
a leak on the left hand side on the door, we have changed the o-ring and
still
the seal leaks on this side of the door. We have polished the door seal to
make
sure there is no major scratches or marks. We are pretty confident that
specimen
exchange port is OK we can pump this down and we see no signs of a leak. Why
are
we focusing our attention on the large o-ring and chamber door. We can
clean
the o-ring with alcohol (methanol or ethanol) and we can rough pump the
chamber
out and put the chamber on the high vac system but after a period of time
the
alcohol dries out and the system shuts down too a poor vacuum (overnight).
Then
we try to rough pump the chamber and we cannot go more than 20-30 mamps
difference on the pirani gauge. We have done this several times with the
same
results. We are going to try to pressurize the system with He and try He
sniffer
to help us maybe pin point the leak. Thanks to all who have responded with
suggestion on finding the leak and if we are successful in finding the leak
I
will post the finding on the server. Again JEOL has been very responsive in
trying to find the leak and they are somewhat limited on this instrument do
to
its environment. Any other suggestion are welcomed.


Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308


----------
From: "Dopeyee-at-aol.com"-at-sparc5.microscopy.com
Sent: Thursday, June 8, 2000 4:28 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: LEAK IN JEOL

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING

I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP

THEY HAVE TO FIX IT!!!!




From daemon Mon Jun 12 14:29:05 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Mon, 12 Jun 2000 08:29:05 -0700 (PDT)
Subject: SEM of liquid crystal

Contents Retrieved from Microscopy Listserver Archives
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Can anyone recommend a book or preferably a website with SEM images of
liquid crystal and or liquid crystal displays?

TX
Pauline Yu
pyu-at-pw.usda.gov
Microscopist Technician
USDA-ARS-WRRC



From daemon Mon Jun 12 16:51:40 2000



From: Norman Michaud :      Norman_Michaud-at-meei.harvard.edu
Date: Mon, 12 Jun 2000 16:09:40 -0400
Subject: TEM lab equipment available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are consolidating two electron microscopy labs in the Boston area and
have some equipment which someone may want.
1) JEOL JEM 100CX electron microscope which has always been under service
contract and is still being used. It is about 20 years old and we would like
to see it in a new home rather than trashing it. You would need to have it
moved.
2)JEOL JEE 4C vacuum evaporator for Carbon. Still under vacuum and yours for
the taking.
3)Durst Laborator 138S floor model enlarger with many condensers and an Agfa
Rapidoprint DD6400 processor, both in very good shape and for sale.
Contact me directly with any questions.


Norman Michaud
Director, Morphology
Mass Eye and Ear Infirmary
Ophthalmology-5th flr.
243 Charles St, Boston, MA 02114
norman_michaud-at-MEEI.Harvard.edu
Tel:617-573-3316; Fax:617-573-4290



From daemon Mon Jun 12 17:59:09 2000



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Tue, 13 Jun 2000 08:48:34 +1000
Subject: Radioactice SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce W. Arey referred in his request to find a vacuum leak in his
JSM840 to 'radioactive particles' inside the chamber of his SEM. I
wonder if he could be more specific, because I feel if we start calling
a SEM as a 'radioactive system' many safety officers will have a field
day. I have been working with various SEMs since 1968 and never felt
that I might be bombarded by radioactive particles, even when I opened
the chamber (e.g. ETEC) of the SEM.



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Mon Jun 12 23:41:44 2000



From: Gillian Bond :      gbond-at-nmt.edu
Date: Mon, 12 Jun 2000 17:43:23 -0600 (MDT)
Subject: Jet polishing of tungsten

Contents Retrieved from Microscopy Listserver Archives
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I have a student who has been trying to jet polish tungsten for TEM. She
has tried various concentrations of sodium hydroxide in water, as well as
40g trisodium phosphate/250ml water, and 55.8g magnesium perchlorate/250ml
methanol, at a range of voltages. We have a Fischione jet-polishing
unit. So far, none of the samples has been close to good. Can anyone
help us out here, with past experience or general suggestions?

Many thanks in advance,

Gill

Dr Gillian M. Bond
Department of Materials & Metallurgical Engineering
New Mexico Tech
Socorro, NM 87801



From daemon Mon Jun 12 23:41:46 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 12 Jun 2000 22:39:40 -0400
Subject: Jet polishing of tungsten

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gill:

Certainly the best reference you can have for any jet polishing inquiry is
Bernie Kestel at Argonne National Laboratory. I will forward this to him
to see if he can add anything else. In digging through my extensive
"Bernie Archives" I did find a paper titled "A Jet Polishing Solution for
Silicon Germanium, Tantalum, Niobium and Tungsten-Rhenium" Ultramicrscopy 9
(1982) 379-384.

He was able to get a good polish under the following conditions:
Temperature: -50 C
Jet Height: 4.5mm (Single vertical jet system)
Pump setting: 6
Volts: 40
Current: 20mA

This was done using his BK-1 solution. BK-1 is prepared by mixing 500ml
methanol, 100ml butyl cellosolve, 90ml H2SO4 and 30ml HF.

He also has another paper MRS Volume 199 "Improved Methods and Novel
Techniques for Jet Electropolishing of TEM Foils" which lists a method for
electropolishing a 0.010" tungsten wire.

He was able to get a good polish under the following conditions:
Temperature: -50 C
Pump setting: 6
Volts: 120V

Using 6% HF, 12% sulphuric acid, 68% methanol, and 14% butyl cellosolve.

Of course these were done with a South Bay Vertical Jet system so you will
need to adjust the parameters for your system. Please get the reference
papers or contact me and I will send them to you. I have a long list of
Bernie's papers I could send you along with many other references on TEM
sample preparation that may be of interest. If you'd like to see more,
please contact me.

DISCLAIMER: South Bay Technology produces the Model 550 D Single Vertical
Jet Electropolisher as described above and, therefore, has a vested
interest in promoting its use.

Best regards-

David
Writing at 7:27:57 PM on 06/12/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Gillian Bond
}
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I have a student who has been trying to jet polish tungsten for TEM. She
has tried various concentrations of sodium hydroxide in water, as well as
40g trisodium phosphate/250ml water, and 55.8g magnesium perchlorate/250ml
methanol, at a range of voltages. We have a Fischione jet-polishing
unit. So far, none of the samples has been close to good. Can anyone
help us out here, with past experience or general suggestions?

Many thanks in advance,

Gill

Dr Gillian M. Bond
Department of Materials & Metallurgical Engineering
New Mexico Tech
Socorro, NM 87801
{



From daemon Mon Jun 12 23:41:48 2000



From: Tommy Davis :      barb29t-at-arabia.com
Date: Mon, 12 Jun 2000 22:42:05 -0500
Subject: Disaster Recovery Planning #5453

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Business continuity and disaster recovery planning has now
been made easier than ever with Version 7.3 of the
Disaster Recovery System (DRS) product. DRS provides a
plan for inaccessibility or inoperability (a disaster situation).

DRS is an industry standard software product, used by thousands
worldwide. DRS users are in large and small companies across
a wide variety of industries.

DRS conforms to federal regulations and meets insurance, auditing
and legal requirements. DRS runs under Windows, with stand-alone
and network versions available. DRS provides the most complete,
easy-to-use product available today.

To prove its value, a free trial is available. For more
information, visit our web site at
www.drsbytamp.com {http://www.drsbytamp.com} .
Dealer and distributor inquiries are welcomed

*****************************************************************
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section 301. Per Section 301, Paragraph (a)(2)(C) of S. 1618,
further transmissions to you by the sender of this email may be
stopped at no cost to you. This message is not intended for
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has been done to the best of our technical ability.If you are a
Washington, Virginia, or California resident please remove
yourself.
====================================================

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From daemon Mon Jun 12 23:41:48 2000



From: Tommy Davis :      barb29t-at-arabia.com
Date: Mon, 12 Jun 2000 22:42:05 -0500
Subject: Disaster Recovery Planning #5453

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Business continuity and disaster recovery planning has now
been made easier than ever with Version 7.3 of the
Disaster Recovery System (DRS) product. DRS provides a
plan for inaccessibility or inoperability (a disaster situation).

DRS is an industry standard software product, used by thousands
worldwide. DRS users are in large and small companies across
a wide variety of industries.

DRS conforms to federal regulations and meets insurance, auditing
and legal requirements. DRS runs under Windows, with stand-alone
and network versions available. DRS provides the most complete,
easy-to-use product available today.

To prove its value, a free trial is available. For more
information, visit our web site at
www.drsbytamp.com {http://www.drsbytamp.com} .
Dealer and distributor inquiries are welcomed

*****************************************************************
This message is sent in compliance of the new email bill
section 301. Per Section 301, Paragraph (a)(2)(C) of S. 1618,
further transmissions to you by the sender of this email may be
stopped at no cost to you. This message is not intended for
residents in the State of WA, CA & VA Screening of addresses
has been done to the best of our technical ability.If you are a
Washington, Virginia, or California resident please remove
yourself.
====================================================

///////////////////////////////////////////////////
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///////////////////////////////////////////////////





From daemon Tue Jun 13 07:32:46 2000



From: Per Hšrstedt :      per.horstedt-at-pathol.umu.se
Date: Tue, 13 Jun 2000 09:17:46 +0200
Subject: Zeiss EM 109 vacuum question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
one of our TEM's , a Zeiss EM 109, is equipped with an ion getter hi-vac
pump. We are now planning to install a turbopump including all the piping
and valves that are necessary. The reason for this is that the high vacuum
is not good enough ( only in low E-6 area ) and the ion pump has to work
too hard and the life-time of the electrodes becomes very short. Also the
housing is clogged with trapped gas molecules and has to be regenerated far
too often.
Technically and electronically this exchange is fairly easily done, but my
question is if anyone has done it and if so, what's the experience?
Yours sincerely

Per Hšrstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of UmeŒ
S-90187 UmeŒ
Sweden

phone int-46-90-7851541
fax int-46-90-7851215



From daemon Tue Jun 13 07:32:49 2000



From: Alexander Mironov :      mironov-at-cmns.mnegri.it
Date: Tue, 13 Jun 2000 11:31:30 +0200 (MET DST)
Subject: About monovalent Fab fragments conjugated with HRP

Contents Retrieved from Microscopy Listserver Archives
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Dear Friends,
For a long time we used Fab fragments-HRP from BioSys (France) for the
immuno EM labelling of proteins with subsequent preembedding. These
fragments were the best. However, recently the company cancelled its
activity and does not send any more these products.
Would you be so kind to tell me what has happened (if you know) and what
fragments have the same quality?

Sincerely yours, Alexander Mironov



From daemon Tue Jun 13 07:32:54 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 13 Jun 2000 08:05:00 -0500
Subject: Re:Zeiss EM 109 vacuum question

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
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I changed my former SEM (Etec w/D.P)to a a Leybold mag-lev turbo a number of
years ago. The ball bearing pumps (of the day) caused too much vibration.
The
results were excellent! The Etec plumbing allowed the turbo to run
continuously
during vent cycles which was of great benefit.

Keep in mind that for any gas, turbos have a fixed compression ratio. Among
other things, the foreline pressure will have a direct influence on the
ultimate
vacuum.

The ion pumos are better than turbos for the cleanest, highest vacuum, but
you
have observed one weakness. They do not excel at pumping large volumes of
garbage-loaded gasses.

Good Luck,

Woody White
McDermott Technology

Hi,
one of our TEM's , a Zeiss EM 109, is equipped with an ion getter hi-vac
pump. We are now planning to install a turbopump including all the piping
SNIP



From daemon Tue Jun 13 07:32:55 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 13 Jun 2000 08:10:00 -0500
Subject: Re:Radioactice SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I believe The SEM to which Bruce refered was/is used to examine radioactive
materials. The "zoomies" are not from the SEM itself, but contamination
from
his specimens. My (former) Etec was in a similar condition. Over the
years, my
work mix resulted in a stage/chamber activitly level in the thousands of cpm
generated by radioactive products of nuclear fission. ...Kept the really
loose
stuff at a minimum, but certainly had to exercise the appropriate
precautions
when working on the system.

Woody White
McDermott Technology
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Bruce W. Arey referred in his request to find a vacuum leak in his
JSM840 to 'radioactive particles' inside the chamber of his SEM. I
wonder if he could be more specific, because I feel if we start calling
a SEM as a 'radioactive system' many safety officers will have a field
day. I have been working with various SEMs since 1968 and never felt
that I might be bombarded by radioactive particles, even when I opened
the chamber (e.g. ETEC) of the SEM.



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Tue Jun 13 07:43:41 2000



From: Alexander Mironov :      mironov-at-cmns.mnegri.it
Date: Tue, 13 Jun 2000 07:33:27 -0500
Subject: TEM. About Fab-HRP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friends,
For a long time we used Fab fragments-HRP from BioSys (France) for the
immuno EM labelling of proteins with subsequent preembedding. These
fragments were the best. However, recently the company cancelled its
activity and does not send any more these products.
Would you be so kind to tell me what has happened (if you know) and what
fragments have the same quality?

Sincerely yours, Alexander Mironov




From daemon Tue Jun 13 08:12:54 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Tue, 13 Jun 2000 09:50:51 +0000
Subject: looking for a critical point drier...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Laboratory of Electron Microscopy is looking for an used critical point
drier to obtain it in donation....
We can pay all the costs of shipping and handling
For any questions, please
contact to me....

best regards....
===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Tue Jun 13 08:12:54 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 13 Jun 2000 14:08:45 +0100 (GMT Daylight Time)
Subject: Re: Zeiss EM 109 vacuum question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Per,

I have done this on a JEOL 4000 but not a Zeiss. I used a
Maglev TMP (Seiko Seiki) and an antivibration bellows to
couple the pump because I was afraid of vibration degrading
the 0.25nm resolution. I need not have worried, even with
the antivibration bellows shorted out I was OK.
Check that the TMP you choose does not give out any
magnetic fields when running.
If vibration is a problem then the Balzers (Pfieiffer)
antivibration bellows that has a large worm drive clip
around them can be tuned (by tightening the clip) to avoid
the instrument resonant frequency.

Good luck,
Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

On Tue, 13 Jun 2000 09:17:46 +0200 Per
=?iso-8859-1?Q?H=F6rstedt?= {per.horstedt-at-pathol.umu.se}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} one of our TEM's , a Zeiss EM 109, is equipped with an ion getter hi-vac
} pump. We are now planning to install a turbopump including all the piping
} and valves that are necessary. The reason for this is that the high vacuum
} is not good enough ( only in low E-6 area ) and the ion pump has to work
} too hard and the life-time of the electrodes becomes very short. Also the
} housing is clogged with trapped gas molecules and has to be regenerated far
} too often.
} Technically and electronically this exchange is fairly easily done, but my
} question is if anyone has done it and if so, what's the experience?
} Yours sincerely
}
} Per Hörstedt
} Department of Medical Biosciences
} Pathology
} Unit for Electron Microscopy
} University of Umeå
} S-90187 Umeå
} Sweden
}
} phone int-46-90-7851541
} fax int-46-90-7851215



From daemon Tue Jun 13 08:22:56 2000



From: Phoebe J Doss :      pjdoss-at-okstate.edu
Date: Tue, 13 Jun 2000 09:32:35 -0500
Subject: help, I've fallen and can't get rid of the precipitate!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello:

After many years of staining grids with uranyl acetate and lead citrate, we
have begun to see a needle like or shard precipitate, (about 1/2 inch long
at 100,000x's; resembles the lead precipitate on page 469 of Electron
Microscopy second edition John Bozzola and Lonnie Russell). We have been
using a 2.5% aqueous uranyl acetate and Reynold's lead citrate (filtered
through a 2 micron filter) for the past 4 years or so with no problems. I
have stained the grids with just UA and can see no precipitate and have
stained grids with just the lead citrate and still not see the precipitate.
I have also checked the water and cannot still see the precipitate.
However, when I stain with UA followed by lead citrate it mysteriously
reappears much to my dissatisfaction. I have also tried the basic lead
citrate and just recently tried Sato's lead stain and had the same problem.
I have made up UA from a newly purchased bottle. I have also lessened the
staining times from 30 minutes in UA to 7 minutes and from 20 minutes in
lead citrate to 5 minutes and the precipitate is less but still there. I
have also checked the grids before staining them and cannot see the
precipitate. Please help, I grow more grey day by day.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Oklahoma State University



From daemon Tue Jun 13 09:42:41 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 13 Jun 2000 10:31:29 -0400 (EDT)
Subject: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Back in the olden days, when BioRad sold microscopy supplies, they had an
epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
epoxies). It was blue gunk in a little jar. Does anyone know what happened
to this stuff? Or have an alternate?

I've just been digging through catalogues and Ted Pella sells a liquid
cleaner - any experience with it?

Thanks!

Tamara Howard
CSHL



From daemon Tue Jun 13 10:02:39 2000



From: Ronald LHerault :      lherault-at-bu.edu
Date: Tue, 13 Jun 2000 10:54:11 -0400 (EDT)
Subject: Streptococci Mutans preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


a student here needs to fix and dehydrate strep mutans on polystyrene
petri dishes. Can anyone point me to a good (simple?) protocol? Since we
are primarily a materials research lab, we don't have a lot of biological
references.

Thanks

Ron L
lherault-at-bu.edu



From daemon Tue Jun 13 11:22:26 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Tue, 13 Jun 2000 12:15:16 -0400
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tamara:

I don't know the Bio-Rad product, but Ladd sold (still sells?) a product called
Met-a-terge that gets rid of uncured resins. A little bit goes a long way.
I've used it for years.

Hope this helps.

Regards,
Bev Maleeff
SmithKline Beecham Pharmaceuticals




From daemon Tue Jun 13 12:12:21 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 13 Jun 2000 13:08:35 -0400
Subject: Transfer of University-owned instrumentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Mansfield's post regarding surplus equipment prompts me to make the
following observations regarding the transfer of University-owned equipment
in the United States. I would imagine that many other countries might have
similar policies.

It often seems to come as a surprise to people to discover that the US
government won't buy the same piece of equipment twice. What I mean by
this is that if a piece of equipment has originally been purchased using
federal funds (regardless of who currently holds title), then another
institution cannot use federal funds (regardless of the source) to buy the
equipment from the first owner.

To use the specific example of John's equipment: suppose it was bought
originally with, say, an NSF grant, and I find that I could make use of it
now. In order to do that I would need to find a non-US-government source
of money, as I could not use even the income from my facility operation
(which is regarded by the accountants as government money, as it originates
predominantly from government research grants).

The logic of this policy, of course, is quite inescapable, however
unpalatable it may be to the present owners of the equipment.

The policy only covers the cost of purchasing the equipment, by the way.
If, to use the same example, John were to give me his surplus equipment, it
would be perfectly acceptable for me to use federal funds to pay for the
packing and shipping (and, if appropriate, reinstallation)

Tony Garratt-Reed.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Tue Jun 13 12:52:15 2000



From: drose-at-wlgore.com
Date: Tue, 13 Jun 2000 13:44:37 -0400
Subject: SEM - sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear List,

I am using a Denton Desk II sputter coater with a Pt target. I have noticed
recently that a dark spot has formed in the middle of the target. I have not
seen this before. Is this due to impurities in the target, problems with the
vacuum, contamination from outgassing samples, or something else? Everything
appears to be running fine and sample types have not changed. Do I clean it or
just leave it alone? What is it telling me (if the coater could talk)?

Thanks in advance for all your expertise.

David BG Rose
WL Gore and Associates
297 BLue Ball Road
Elkton, MD 21921
410-506-2958




From daemon Tue Jun 13 13:23:51 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 13 Jun 2000 13:13:50 -0500
Subject: help, I've fallen and can't get rid of the precipitate!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phoebe,

We recently had a rash of horrible precipitate problems that we couldn't
seem to trace. Our staining procedures are similar to yours. We made up
fresh stains, changed all our syringe filters, used every precaution we
could think of.

Then we had the water system checked. Our in-line reverse-osmosis,
deionization system had become a mess, although we had assumed (there's that
word!) that the company we leased it from was maintaining it properly.
Turns out that they thought we owned the system, while in fact we only
rented it and paid them to service it.

Anyway, to keep it short, we purchased a Millipore bench-top, low-volume
water-polishing unit and used our old water to feed it (after getting the
thing serviced properly). Our precipitates disappeared and have not yet
reappeared.

For what it's worth.

(No financial interest in Millipore, etc.)

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Phoebe J Doss [mailto:pjdoss-at-okstate.edu]
Sent: Tuesday, June 13, 2000 9:33 AM
To: microscopy-at-sparc5.microscopy.com


Hello:

After many years of staining grids with uranyl acetate and lead citrate, we
have begun to see a needle like or shard precipitate, (about 1/2 inch long
at 100,000x's; resembles the lead precipitate on page 469 of Electron
Microscopy second edition John Bozzola and Lonnie Russell). We have been
using a 2.5% aqueous uranyl acetate and Reynold's lead citrate (filtered
through a 2 micron filter) for the past 4 years or so with no problems. I
have stained the grids with just UA and can see no precipitate and have
stained grids with just the lead citrate and still not see the precipitate.
I have also checked the water and cannot still see the precipitate.
However, when I stain with UA followed by lead citrate it mysteriously
reappears much to my dissatisfaction. I have also tried the basic lead
citrate and just recently tried Sato's lead stain and had the same problem.
I have made up UA from a newly purchased bottle. I have also lessened the
staining times from 30 minutes in UA to 7 minutes and from 20 minutes in
lead citrate to 5 minutes and the precipitate is less but still there. I
have also checked the grids before staining them and cannot see the
precipitate. Please help, I grow more grey day by day.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Oklahoma State University



From daemon Tue Jun 13 13:23:51 2000



From: Snow, David B. :      snowdb-at-pweh.com
Date: Tue, 13 Jun 2000 13:17:57 -0500
Subject: Transmission Electron Microscopist (TEM) / Engineer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Transmission Electron Microscopist (TEM) / Engineer

United Technologies Corporation is seeking an engineer to fill the TEM
operator/engineer position at the United Technologies Research Center in
East Hartford, CT. This position will provide support to the United
Technologies Corporation Business Units including Pratt & Whitney, Carrier,
Sikorsky Aircraft, Hamilton Sundstrand, Otis Elevator, and International
Fuel Cells. The TEM operator will be responsible for the dailyoperations of
the TEM laboratory; including preparation of TEM samples using various
techniques such as dimpling, ion milling, jet polishing, microtoming, and
replication. Project duties include conducting failure analyses,
characterization of surface coatings, and analysis of advanced metal and
ceramic materials. The candidate should have experience with both TEM
sample preparation and conventional TEM operation. Good communication and
interpersonal skills are essential. The ability to recognize fracture modes
and origins of fractures is desired. Experience with Scanning Electron
Microscopy (SEM) is a plus.

Qualified candidates will have a BS in Materials Science or equivalent, with
a minimum of 2 years TEM experience. U. S. citizenship or permanent
residency is required.

Please visit our web site at www.utrc.utc.com, and send your resume to
Employment Opportunities, Code MATS-2050-9049, 411 Silver Lane, East
Hartford, CT 06118 or e-mail employment-at-utrc.utc.com. United Technologies
Corporation is an equal opportunity employer.





From daemon Tue Jun 13 16:39:45 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 14 Jun 2000 08:27:16 GMT+1200
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Tamara

}
} Back in the olden days, when BioRad sold microscopy supplies, they had an
} epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} epoxies). It was blue gunk in a little jar. Does anyone know what happened
} to this stuff? Or have an alternate?
}
} I've just been digging through catalogues and Ted Pella sells a liquid
} cleaner - any experience with it?
}

I seem to remember that soaking in N,N-dimethyl formamide dissolves
epoxy, you might care to beg a little from a freiendly chemistry dept
and try it, it's not very expensive. It has a moderately offensive
smeel, though.

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Jun 13 16:39:46 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Tue, 13 Jun 2000 16:35:42 -0400
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tamara, Beverly and all other interested parties,

Yes, Ladd stills sells Met-A-Terge (catalog #13045).
Please check our web site http://www.laddresearch.com
for more information or contact me off line.

JD Arnott

Disclaimer: As stated above, Ladd sell Met-A-Terge and thus has a
commercial interest in this thread.

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com


Beverly_E_Maleeff-at-sbphrd.com-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Tamara:
}
} I don't know the Bio-Rad product, but Ladd sold (still sells?) a product called
} Met-a-terge that gets rid of uncured resins. A little bit goes a long way.
} I've used it for years.
}
} Hope this helps.
}
} Regards,
} Bev Maleeff
} SmithKline Beecham Pharmaceuticals

--



From daemon Tue Jun 13 16:39:47 2000



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Tue, 13 Jun 2000 13:38:18 -0700
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tamara,
I have used the Ted Pella Epoxy Hand Cleaner and it works well. It will
remove epoxy from hands and also glassware.
Jo Dee Fish

PS I am not affiliated with Ted Pella, just love their products!

Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Back in the olden days, when BioRad sold microscopy supplies, they had an
} epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} epoxies). It was blue gunk in a little jar. Does anyone know what happened
} to this stuff? Or have an alternate?
}
} I've just been digging through catalogues and Ted Pella sells a liquid
} cleaner - any experience with it?
}
} Thanks!
}
} Tamara Howard
} CSHL

--
Jo Dee Fish
Electron Microscopy Assistant
Cell Analysis Facility
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
(858)646-3100 ext. 3620




From daemon Tue Jun 13 16:39:51 2000



From: Connie McManus :      conmac-at-cc.usu.edu
Date: Tue, 13 Jun 2000 15:29:58 -0600
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My husband uses epoxy on a sailboat he's building. He cleans everything
up... hands, spills, etc... with plain old vinegar. We go through a LOT of
vinegar. If it's dried, then soak acetone on it until it softens, then use
vinegar (or 5% acetic acid) to mop up the residues.

connie m

At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} Back in the olden days, when BioRad sold microscopy supplies, they had an
} epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} epoxies). It was blue gunk in a little jar. Does anyone know what happened
} to this stuff? Or have an alternate?
}
} I've just been digging through catalogues and Ted Pella sells a liquid
} cleaner - any experience with it?
}
} Thanks!
}
} Tamara Howard
} CSHL
}
}
}
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA



From daemon Tue Jun 13 22:04:13 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 13 Jun 2000 16:26:55 -0700
Subject: RE: Transfer of University-owned instrumentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Anthony Garratt-Reed writes ...

} To use the specific example of John's equipment: suppose
} it was bought originally with, say, an NSF grant, and
} I find that I could make use of it now. In order to do
} that I would need to find a non-US-government source
} of money, as I could not use even the income from my
} facility operation (which is regarded by the accountants
} as government money, as it originates
} predominantly from government research grants).

I would assume it can even get messy if, at least some, of my
facility's income had come from outside sources. I would imagine the
accountants will first assume it is government $$ ... in which case I
would have to show I had taken in an equivelent in outside $$ ... but
over what time frame? ... this fiscal? ... last 10 years?

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Jun 13 22:04:28 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 13 Jun 2000 19:51:28 -0700
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use methylene dichloride (dimethyl chloride) to dissolve most any epoxy.

I have 3-5 gallons of it. I do not know if it is still generally available.

gg

At 07:31 AM 6/13/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jun 13 22:54:38 2000



From: earlw-at-pacbell.net
Date: Tue, 13 Jun 2000 20:43:51 -0700
Subject: Re: MSA Listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Joe,

The listserver is at MSA listserver {Microscopy-at-sparc5.microscopy.com}
Just sent email say, "Please subscribe".
It is fairly active but is only about 1/2 relevant as most data is biology
oriented.

Earl
JCNABITY-at-aol.com wrote:

} Dear Earl,
}
} Could you tell me how to subscribe to the MSA listserver? Greg talked highly
} of it and I'm thinking I will set up another e-mail account to use for it.
} Since it is pretty active, I didn't want all the messages going in with my
} normal e-mail, but a using a separate account will resolve that issue.
}
} Joe



From daemon Wed Jun 14 07:47:22 2000



From: mohamed a jaffer :      mohamed-at-molbiol.uct.ac.za
Date: Wed, 14 Jun 2000 13:45:54 SAST-2
Subject: 70mm roll film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List

Our EM Unit has a Zeiss EM109 which uses 70mm roll film - Agfa
Scientia 23D56. Manufacture of this film ceased quite a while back.
We are trying to locate unused stock of this film for our usage. If
you have any surplus stock for sale please contact me.

Thanks
Mohamed

******************************
M. A. Jaffer
Electron Microscope Unit
R. W. James Building
University of Cape Town
Private Bag
Rondebosch, 7701
South Africa

Tel: +27-21-6503354
fax: +27-21-6891528

e-mail: mohamed-at-molbiol.uct.ac.za
***************************************************



From daemon Wed Jun 14 08:50:44 2000



From: Connie McManus :      conmac-at-cc.usu.edu
Date: Wed, 14 Jun 2000 07:47:42 -0600
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 06:09 PM 06/13/2000 -0700, Don Hammer wrote:
} Stuff is cheap too and if there is any left after the zillions of home uses,
} great on salads!!!!

yeah, especially the used stuff........ eeeeuewwwwwwww! *G*

connie m
}
} Don Hammer, Retired Guy
} ----- Original Message -----
} From: Connie McManus {conmac-at-cc.usu.edu}
} To: Tamara Howard {howard-at-cshl.org} ; Microscopy Listserver
} {Microscopy-at-sparc5.microscopy.com} ; Histology listserver
} {histonet-at-pathology.swmed.edu}
} Sent: Tuesday, June 13, 2000 2:29 PM
} Subject: Re: Epoxy cleaner?
}
}
} } My husband uses epoxy on a sailboat he's building. He cleans everything
} } up... hands, spills, etc... with plain old vinegar. We go through a LOT
} of
} } vinegar. If it's dried, then soak acetone on it until it softens, then
} use
} } vinegar (or 5% acetic acid) to mop up the residues.
} }
} } connie m
} }
} } At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} } } Back in the olden days, when BioRad sold microscopy supplies, they had an
} } } epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} } } epoxies). It was blue gunk in a little jar. Does anyone know what
} happened
} } } to this stuff? Or have an alternate?
} } }
} } } I've just been digging through catalogues and Ted Pella sells a liquid
} } } cleaner - any experience with it?
} } }
} } } Thanks!
} } }
} } } Tamara Howard
} } } CSHL
} } }
} } }
} } }
} } Connie McManus
} } Veterinary Diagnostics Lab
} } Utah State University
} } Logan, UT
} } USA
} }
} }
}
}
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA



From daemon Wed Jun 14 15:38:17 2000



From: veys-at-bota.ucl.ac.be (Pascal Veys)
Date: Wed, 14 Jun 2000 16:18:08 +0100
Subject: wax and lipid droplets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi to all,
Does anybody have some information on a possible relationship between lipid
droplets and wax production in plant cells. Are there some evidences that
lipid droplets could actually be storage sites of wax precursors ? I
looked for that in literature but found nothing...
Thus : HELP !
References will be welcome
Thanks in advance for answering
Bye
Pascal

""""""""'
( O)(o )
--------------------0000--------------0000----------
Pascal VEYS
Laboratory of Plant Biology
Catholic University of Louvain
Place Croix du Sud 5 (bte 14)
B 1348 Louvain-la-Neuve
Belgium
Phone : 0032 10473004
Fax : 0032 10473471
Email : Veys-at-bota.ucl.ac.be
---------------------ooooO-----------Ooooo--------
( ) (_)(_) ( )
) ( ) (
(_) (_)




From daemon Wed Jun 14 15:38:17 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 14 Jun 2000 11:40:12 -0500
Subject: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
===============================================================
I use methylene dichloride (dimethyl chloride) to dissolve most any epoxy.
I have 3-5 gallons of it. I do not know if it is still generally available.
================================================================
If we are talking about methylene dichloride, or dichloromethane, CAS # 75-
09-2, this is a pretty bad actor, and is on the list of Prop. 65 chemicals
for the State of California as being cancer causing. Chemicals on the Prop
. 65 list are so highly restricted that in some organizations, they are
allowed in only with the approval of top management.

But I do have a question: I always thought that most epoxies, certainly the
ones used in microscopy, ended up being three dimensionally crosslinked
intractable solids. The only way such a material is going to be "dissolved"
is for chemical bonds to be broken. And yet, I don't see how chemically,
methylene dichloride is going to be breaking chemical bonds. Or the same
comment for some of the other materials mentioned. These materials might
plasticize (e.g. soften) an epoxy and aid in its removal from a surface, but
do any of these really "dissolve" a three dimensionally crosslinked epoxy
system?

I am very interested in this topic because we believe that at least in terms
of getting epoxy out of the "nooks and crannies" of a non-smooth surface, an
oxygen plasma is needed. But perhaps we are wrong about that, that is why
I ask the question.

Disclaimer: SPI Supplies manufactures the Plasma Prep™ II plasma etcher and
has an interest in seeing more applications for plasma etching.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================








From daemon Wed Jun 14 15:38:19 2000



From: NPGSlithography-at-aol.com
Date: Wed, 14 Jun 2000 13:28:54 EDT
Subject: Please Subscribe

Contents Retrieved from Microscopy Listserver Archives
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Please Subscribe



From daemon Wed Jun 14 15:38:21 2000



From: Joyce Craig :      rcraig8-at-crown.icongrp.com
Date: Wed, 14 Jun 2000 13:50:24 -0500
Subject: SEM-sputter coater

Contents Retrieved from Microscopy Listserver Archives
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David, I had the same problem with a gold target-a dark spot in the
middle. Also, what was being sputtered on my samples was not gold. I
cleaned the target with acetone and it has been working fine since.
Joyce Craig
Chicago State University



From daemon Wed Jun 14 15:38:22 2000



From: Marsha Pilgrim :      mpilgrim-at-MendelBio.COM
Date: Wed, 14 Jun 2000 11:51:50 -0700
Subject: uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Can anyone recommend a supplier for uranyl acetate?

Also, does anyone out there have experience with Sigma's Lowicryl kit?

If possible, please respond to me directly at: mpilgrim-at-mendelbio.com

Many thanks,
Marsha



From daemon Wed Jun 14 15:38:23 2000



From: Joyce Craig :      rcraig8-at-crown.icongrp.com
Date: Wed, 14 Jun 2000 14:41:55 -0500
Subject: TEM cell cultures

Contents Retrieved from Microscopy Listserver Archives
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We are working with Leishmania major, a parasite that is incorporated
into macrophages. We have had good success with fixation of lymph nodes
infected with Leishmania.
We have not been as happy with the results of fixation of cell cultures
of infected bone marrow macrophages. The membranes of the Leishmania
and of the internal compartments within the macrophages that hold the
Leishmania are well fixed, but the external cell membranes of the
macrophages are somewhat discontiuous. We have not done cell cultures
before. Is this a problem to be expected? We are fixing with 2+2
glutaraldehyde/paraformaldehyde in 0.1 M phosphate buffer, post-fixing
with 2% buffered Osmium, dehydrating with ethanol and propylene oxide,
then embedding in epoxy. We have shortened all times compared to those
we use with tissue samples.
Joyce Craig
Chicago State University



From daemon Wed Jun 14 18:25:47 2000



From: Subramanian Karthikeyan :      karthik-at-micro.uoguelph.ca
Date: Wed, 14 Jun 2000 15:38:48 -0500
Subject: non-florescent plastic coverslip..

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I am looking for a non-fluorescent plastic coverslip that allows
confocal laser microscopy and subsequent sectioning for
Transmission Electron Microscopy. I will greatly appreciate your
suggestions.

Sincerely,
Karthi Subramanian
Department of Microbiology
University of Guelph
Guelph Ontario N1G 2W1
Phone: (519)824-4120 ext.8904
Fax:(519)837-1802




From daemon Wed Jun 14 18:25:48 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 14 Jun 2000 15:46:01 -0500
Subject: RE: Stereometry by computer

Contents Retrieved from Microscopy Listserver Archives
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I perform stereo measurements only occasionally,
so I prefer to save money on specialized equipment or
software. All I use is just freeware program ImageTool
(good for on-screen stereo pair measurements) and Excel
(not bad for calculations).

Of course, for a big project it's better to buy a software.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
} Sent: Thursday, June 08, 2000 11:13 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Stereometry by computer
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?
}
} Thank you.
}
} John B.
}
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}



From daemon Wed Jun 14 18:25:48 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 14 Jun 2000 10:48:50 -1000 (HST)
Subject: TEM labeling Si-OH groups?

Contents Retrieved from Microscopy Listserver Archives
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Oh wise and helpful microscopists-

I need to label Si-OH groups with something that will show up in TEM. If
it were proteins or sugars, I'd immuno- or lectin label. Any ideas how I
can get gold or ferritin or whatever onto these groups?

Mahalo!
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jun 14 18:25:48 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Wed, 14 Jun 2000 17:03:40 -0400
Subject: Re: uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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Marsha Pilgrim wrote:

} Hello,
}
} Can anyone recommend a supplier for uranyl acetate?
} Also, does anyone out there have experience with Sigma's Lowicryl kit?
} If possible, please respond to me directly at: mpilgrim-at-mendelbio.com
}
} Many thanks,
} Marsha


Dear Marsha,

We at Ladd Research, and most of the other supply companies, can sell
you this. In our case it is catalog # 23620 and more information can be
found on our web site, http://www.laddresearch.com

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com



From daemon Wed Jun 14 18:25:49 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Wed, 14 Jun 2000 17:49:03 -0500
Subject: autofluorescence of plant material

Contents Retrieved from Microscopy Listserver Archives
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Help - A faculty member's plant tissue is autofluorescing. She is using
aniline blue to look at pollen tubes (through the styles). She would like
to reduce the background fluorescence. I gave her a copy of a borohyride
reference from a '97 listserv posting. Can anyone recommend a fixation that
will decrease or eliminate the autofluorescence? Any thoughts on this
matter would be greatly appreciated.
thanks,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************




From daemon Wed Jun 14 18:25:50 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Wed, 14 Jun 2000 16:47:36 -0500 (CDT)
Subject: Re: LR White flat embedding

Contents Retrieved from Microscopy Listserver Archives
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It took me a few days to find this protocol from my collegue Lucinda
Swatzell. It sounded intriguing. Although I've not tried it myself yet,
she's used it with success.

I've copied the following out of her e-mail. Things that need to be kept
in mind are that she polymerizes in a vacuum oven, she's refering to plant
seedlings, and that I don't have any financial interest that I know of
(i.e. I haven't checked my mutual fund prospectus) in Rubbermaid, Inc.

Here it is:

"There is a way to flat embed with LR White: This works great for me when
I am keeping them on their agar blocks and maintaining orientation, but it
should also work for regular seedlings to keep them flat instead of
crooked in the bottom of the capsules. Use rubbermaid ice cube trays.
Place the specimens in the flat bottoms of the tray. cover with about 1/4
in of resin. In each well, place another well that has been cut from it's
tray. The single loose wells will nestle down into the ice cub tray on
top of the resin. Because rubbermaid is dishwasher safe it will take the
heat, but get soft enough to snuggle in tightly and keep out oxygen. When
you pump the vaccuum the extra resin also snakes up into the cracks, so
that you get a seal. Now the thing that is scary: will loose seedlings
just suck up into the cracks? I haven't tried them."

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816





From daemon Wed Jun 14 18:25:50 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 14 Jun 2000 15:07:34 -0700
Subject: Re: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
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I've used it often to unpackage epoxy microchips. These
epoxies are silicone/epoxy and not true epoxy. They are
typically called plastic packaged ICs. Whatever. I've
used MEC and DiMEC to do this. Some packages
had to be oxygen ion blasted open. Over time, the plasma
method has been much safer for the operator and the chip.
And it can make the removal process much faster. Plasma
is the current method of choice.

Whether the "epoxies" talked about here are the same as
those for IC packages is likely not to be.

gary g.



At 09:40 AM 6/14/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 14 18:25:51 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 14 Jun 2000 18:26:55 -0400
Subject: RE: Jet polishing of tungsten

Contents Retrieved from Microscopy Listserver Archives
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I used to prepare W FIM emitter tips with a 5% NaOH solution and I think the
voltage was around 5 to 10 volts ac. When I used to electropolish, the FIM
solutions and conditions were frequently similar to the jet polishing
solutions.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Gillian Bond [mailto:gbond-at-nmt.edu]
} Sent: Monday, June 12, 2000 7:43 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Jet polishing of tungsten
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} I have a student who has been trying to jet polish tungsten
} for TEM. She
} has tried various concentrations of sodium hydroxide in
} water, as well as
} 40g trisodium phosphate/250ml water, and 55.8g magnesium
} perchlorate/250ml
} methanol, at a range of voltages. We have a Fischione jet-polishing
} unit. So far, none of the samples has been close to good. Can anyone
} help us out here, with past experience or general suggestions?
}
} Many thanks in advance,
}
} Gill
}
} Dr Gillian M. Bond
} Department of Materials & Metallurgical Engineering
} New Mexico Tech
} Socorro, NM 87801
}
}



From daemon Thu Jun 15 07:33:49 2000



From: Thomas Neumann :      tneumann-at-u.washington.edu
Date: Wed, 14 Jun 2000 16:43:29 -0700
Subject: Artifact or Structured Water?

Contents Retrieved from Microscopy Listserver Archives
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My name is Tim Strovas and I am a graduate student at the U of
Washington bioengineering department. I am investigating the halo
effect from single myofibrils (from bumblebee muscle tissue) as seen
under a phase contrast microscope. Has anyone encountered a previous
investigation into this effect and its possible relationship with
structured water?
Note: The Halo does not appear as ripples that are normally associated
with optical microscope artifacts. The halo is single broad band that
borders the tissue sample.




From daemon Thu Jun 15 07:33:49 2000



From: Thomas Neumann :      tneumann-at-u.washington.edu
Date: Wed, 14 Jun 2000 16:54:35 -0700
Subject: Artifact or Structured Water?

Contents Retrieved from Microscopy Listserver Archives
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Sorry, forgot return address: T-Strovas-at-Cornell-Iowa.edu

My name is Tim Strovas and I am a graduate student at the U of
Washington bioengineering department. I am investigating the halo
effect from single myofibrils (from bumblebee muscle tissue) as seen
under a phase contrast microscope. Has anyone encountered a previous
investigation into this effect and its possible relationship with
structured water?
Note: The Halo does not appear as ripples that are normally associated
with optical microscope artifacts. The halo is single broad band that
borders the tissue sample.





From daemon Thu Jun 15 07:33:50 2000



From: Thomas Neumann :      tneumann-at-u.washington.edu
Date: Wed, 14 Jun 2000 16:54:23 -0700
Subject: Artifact or Structured Water?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, forgot return address: T-Strovas-at-Cornell-Iowa.edu

My name is Tim Strovas and I am a graduate student at the U of
Washington bioengineering department. I am investigating the halo
effect from single myofibrils (from bumblebee muscle tissue) as seen
under a phase contrast microscope. Has anyone encountered a previous
investigation into this effect and its possible relationship with
structured water?
Note: The Halo does not appear as ripples that are normally associated
with optical microscope artifacts. The halo is single broad band that
borders the tissue sample.





From daemon Thu Jun 15 07:33:50 2000



From: =?iso-2022-jp?B?GyRCI1IjaSN6I2EjbBsoSg==?=
Date: Thu, 15 Jun 2000 09:08:35 +0900
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Jun 15 07:33:51 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Thu, 15 Jun 2000 11:35:56 +1000
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day folks,
Be very wary of any Good Epoxy Solvent, do not use it to clean epoxy off
your skin. Anything that is a good solvent for epoxy will probably be a
good solvent for the oils and lipids in/on your skin. These oils and
lipids are your protection against epoxy resins entering your body.
Remember, all epoxy resins at carcinogenic, soap and water are probably
the safest agents to remove epoxies from your skin.
Regards
JVN

Connie McManus wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 06:09 PM 06/13/2000 -0700, Don Hammer wrote:
} } Stuff is cheap too and if there is any left after the zillions of home uses,
} } great on salads!!!!
}
} yeah, especially the used stuff........ eeeeuewwwwwwww! *G*
}
} connie m
} }
} } Don Hammer, Retired Guy
} } ----- Original Message -----
} } From: Connie McManus {conmac-at-cc.usu.edu}
} } To: Tamara Howard {howard-at-cshl.org} ; Microscopy Listserver
} } {Microscopy-at-sparc5.microscopy.com} ; Histology listserver
} } {histonet-at-pathology.swmed.edu}
} } Sent: Tuesday, June 13, 2000 2:29 PM
} } Subject: Re: Epoxy cleaner?
} }
} }
} } } My husband uses epoxy on a sailboat he's building. He cleans everything
} } } up... hands, spills, etc... with plain old vinegar. We go through a LOT
} } of
} } } vinegar. If it's dried, then soak acetone on it until it softens, then
} } use
} } } vinegar (or 5% acetic acid) to mop up the residues.
} } }
} } } connie m
} } }
} } } At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} } } } Back in the olden days, when BioRad sold microscopy supplies, they had an
} } } } epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} } } } epoxies). It was blue gunk in a little jar. Does anyone know what
} } happened
} } } } to this stuff? Or have an alternate?
} } } }
} } } } I've just been digging through catalogues and Ted Pella sells a liquid
} } } } cleaner - any experience with it?
} } } }
} } } } Thanks!
} } } }
} } } } Tamara Howard
} } } } CSHL
} } } }
} } } }
} } } }
} } } Connie McManus
} } } Veterinary Diagnostics Lab
} } } Utah State University
} } } Logan, UT
} } } USA
} } }
} } }
} }
} }
} Connie McManus
} Veterinary Diagnostics Lab
} Utah State University
} Logan, UT
} USA

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Thu Jun 15 07:34:12 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 15 Jun 2000 06:38:06 -0500
Subject: Re: TEM labeling Si-OH groups?

Contents Retrieved from Microscopy Listserver Archives
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I suggest you copy this message to the plant surfaces mail list

To join, send the command
join plant-surfaces firstname lastname
to: mailbase-at-mailbase.ac.uk
"Firstname" can be one or more names or initials. The last
word in this command will be interpreted as the last name.
The email address will be extracted automatically from the
message.

Chris Jeffree

Date sent: Wed, 14 Jun 2000 16:18:08 +0100
To: microscopy-at-sparc5.microscopy.com
} From: veys-at-bota.ucl.ac.be (Pascal Veys)


Hello Tina:

I would approach your problem by either modifying the Si-OH groups with a
hapten and detecting with antibody- or streptavidin-gold, or converting
them to amines or thiols then labeling with a gold labeling reagent
(disclaimer - we make gold labeling reagents). You could introduce amino-
groups at the Si-OH groups using a silylating reagent such as
3-{Tris[2-(2-methoxyethoxy)ethoxy]silyl}propylamine or
3-[Tris(trimethylsiloxy)silyl]propylamine (both from Fluka), then either
biotinylate with NHS-biotin and detect with streptavidin-gold, or label the
amines with Mono-Sulfo-NHS-Nanogold.

I have not actually tried this, and since I don't know what types of
samples you are looking at, it's difficult to say what else in them might
affect the reaction. If there are already other primary amines in your
sample, they need to be blocked first.

If you would like other ideas, a text on solid-phase oligo- or peptide
synthesis might be another good starting point - the chemistry used to
functionalize the beads used in these systems may also be transferable to
your situation.

Hope this is helpful,

Rick Powell


}
} Oh wise and helpful microscopists-
}
} I need to label Si-OH groups with something that will show up in TEM. If
} it were proteins or sugars, I'd immuno- or lectin label. Any ideas how I
} can get gold or ferritin or whatever onto these groups?
}
} Mahalo!
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

**********************************************************************
* NANOPROBES, Incorporated | Tel: (919) 510-0590 *
* 95 Horse Block Road | Fax: (919) 510-0590 *
* Yaphank, NY 11980-9710, | rpowell-at-nanoprobes.com *
* USA | www.nanoprobes.com *
**********************************************************************




From daemon Thu Jun 15 20:52:27 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 15 Jun 2000 22:57:27 +1000
Subject: RE: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Nailon is making a very good argument for using gloves or working very
cleanly.
All epoxies I understand are "somewhat" carcinogenic. The much quoted John
Luft, years ago advised me that photographic fixer (sodium thiosulphate)
solution, chemically changed epoxies so they would not be carcinogenic. If he
was right, then first washing any body parts contaminated by epoxy resin in
photographic fixer should avert the worse. Those fixers do not dissolve or
clean epoxies.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, June 15, 2000 11:36 AM, John Nailon
[SMTP:mmjnailo-at-dingo.cc.uq.edu.au] wrote:
}
} G'day folks,
} Be very wary of any Good Epoxy Solvent, do not use it to clean epoxy off
} your skin. Anything that is a good solvent for epoxy will probably be a
} good solvent for the oils and lipids in/on your skin. These oils and
} lipids are your protection against epoxy resins entering your body.
} Remember, all epoxy resins at carcinogenic, soap and water are probably
} the safest agents to remove epoxies from your skin.
} Regards
} JVN
}
} Connie McManus wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } At 06:09 PM 06/13/2000 -0700, Don Hammer wrote:
} } } Stuff is cheap too and if there is any left after the zillions of home
} } } uses,
} } } great on salads!!!!
} }
} } yeah, especially the used stuff........ eeeeuewwwwwwww! *G*
} }
} } connie m
} } }
} } } Don Hammer, Retired Guy
} } } ----- Original Message -----
} } } From: Connie McManus {conmac-at-cc.usu.edu}
} } } To: Tamara Howard {howard-at-cshl.org} ; Microscopy Listserver
} } } {Microscopy-at-sparc5.microscopy.com} ; Histology listserver
} } } {histonet-at-pathology.swmed.edu}
} } } Sent: Tuesday, June 13, 2000 2:29 PM
} } } Subject: Re: Epoxy cleaner?
} } }
} } }
} } } } My husband uses epoxy on a sailboat he's building. He cleans everything
} } } } up... hands, spills, etc... with plain old vinegar. We go through a LOT
} } } of
} } } } vinegar. If it's dried, then soak acetone on it until it softens, then
} } } use
} } } } vinegar (or 5% acetic acid) to mop up the residues.
} } } }
} } } } connie m
} } } }
} } } } At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} } } } } Back in the olden days, when BioRad sold microscopy supplies, they had
} } } } } an
} } } } } epoxy cleaner (to remove epoxies from hands, benches, etc., not made
} } } } } from
} } } } } epoxies). It was blue gunk in a little jar. Does anyone know what
} } } happened
} } } } } to this stuff? Or have an alternate?
} } } } }
} } } } } I've just been digging through catalogues and Ted Pella sells a liquid
} } } } } cleaner - any experience with it?
} } } } }
} } } } } Thanks!
} } } } }
} } } } } Tamara Howard
} } } } } CSHL
} } } } }
} } } } }
} } } } }
} } } } Connie McManus
} } } } Veterinary Diagnostics Lab
} } } } Utah State University
} } } } Logan, UT
} } } } USA
} } } }
} } } }
} } }
} } }
} } Connie McManus
} } Veterinary Diagnostics Lab
} } Utah State University
} } Logan, UT
} } USA
}
} --
} ****************************************************
} John V Nailon
} Operations Manager
} Centre for Microscopy and Microanalysis
} The University of Queensland
} St. Lucia Queensland 4072
} Phone: +61-7-3365-4214
} Fax: +61-7-3365-4422
} WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
} ****************************************************



From daemon Thu Jun 15 20:52:28 2000



From: Smartech :      smartech-at-javanet.com
Date: Thu, 15 Jun 2000 10:02:36 -0400
Subject: SEM, How to interpret a fractured adhesive film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I needed to have a cross sectional view into an adhesive layer so that I
could count the layers, if possible. Since the adhesive acts like a highly
viscous liquid, polishing it is out of the question. Instead, I submerged
the film into liquid Nitrogen and cut it quickly w/ a scissors. The sound
of the cut was more like a breaking sound. When I examined the cross
sectional surface I observed a brain like surface. There were columns with
a highly consistent diameter and orientation. It looked crystalline. There
was zero evidence of material smearing that one would expect if one cut a
material. Is the proper interpretation that these columns existed before
the fracture and that the fracture occurred along the boundaries? Or is
the cross sectional surface generated by a rippled distortion of an highly
viscous liquid? The regularity of the surface seems to make the latter
interpretation unlikely.

I would be interested in any opinions on the interpretation of the image or
alternative means of cross sectioning the adhesive layer.

I could e-mail an image to anyone who is interested.

Thanks

Ric



From daemon Thu Jun 15 20:52:30 2000



From: diane.a.ciaburri-at-gdds.com
Date: 6/14/00 3:07 PM
Subject: Re: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary and others,

We haven't had a need to unpackage 'plastic' encapsulated IC's for a while but
may have a need to do so soon (preferrably not impairing functionality?!). I am
told that the last person to do this here (now retired) dripped fuming sulfuric
acid on the plastic and used frequent water rinses. We have a plasma etcher but
I was afraid it would take forever to get through the plastic.

Any hints and suggestions would be greatly appreciated.

Diane Ciaburri
Senior Materials Engineer
General Dynamics
100 Plastics Ave.
Pittsfield MA 01210

____________________Forward Header_____________________

I've used it often to unpackage epoxy microchips. These
epoxies are silicone/epoxy and not true epoxy. They are
typically called plastic packaged ICs. Whatever. I've
used MEC and DiMEC to do this. Some packages
had to be oxygen ion blasted open. Over time, the plasma
method has been much safer for the operator and the chip.
And it can make the removal process much faster. Plasma
is the current method of choice.

Whether the "epoxies" talked about here are the same as
those for IC packages is likely not to be.

gary g.




From daemon Thu Jun 15 20:52:30 2000



From: Hao Li :      haoli-at-glue.umd.edu
Date: Thu, 15 Jun 2000 10:40:15 -0700
Subject: information about MgAl2O4

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Hi, All:

This one is not really related to microscopy. But since I am a TEM guy, I
hope I can get some help here.

Does anybody have some information about MgAl2O4 as a substrate for
perovskite films? I know it is not often used, so I am worndering if it has
a major disadvantage so that nobody is using it. Any references would be
welcome.


Thanks a lot

Hao Li



From daemon Thu Jun 15 20:52:31 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 15 Jun 2000 07:55:38 -0700
Subject: Polaron E5200 control board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a control PC board for the E5200 sputter coater.
This is the model with an Intel single chip MPU on one
end and a 4-conductor socket on the other end. The
board uses a VME connector for main interface.

Coater is trashed. Board is OK. If anybody can use
the board, first request gets it.

gary g.



From daemon Thu Jun 15 20:52:33 2000



From: Andy Horsewell :      horsewell-at-ipt.dtu.dk
Date: Thu, 15 Jun 2000 19:05:03 +0200
Subject: please unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please unsubscribe



From daemon Thu Jun 15 20:52:34 2000



From: Mary C. Pfauth :      mpfauth-at-teleport.com
Date: Thu, 15 Jun 2000 09:53:28 -0700 (PDT)
Subject: Re: autofluorescence of plant material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Beth, more details are needed on how the tissue was processed before
viewing. I have used aniline blue to view pollen tubes in style of
Salicornia virginica which had ben fixed in Nawashin's fixative. Have also
viewed tubes of Melilotus which had simply been preserved in 70% EtOH. If
one uses glut as a fixative, it fluoresces so you won't be able to
distinguish the PT from everything else. Mary Pfauth

John P.B. & Mary
mpfauth-at-teleport.com



From daemon Thu Jun 15 20:52:35 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: 6/14/00 3:07 PM
Subject: Re: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Can't comment on sulfuric, but I have used red fuming nitric at near boiling
temperature. Apply acid, let react. Flush witn more acid, let react, etc.

Woody White

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Gary and others,

We haven't had a need to unpackage 'plastic' encapsulated IC's for a while
but
may have a need to do so soon (preferrably not impairing functionality?!).
I am
told that the last person to do this here (now retired) dripped fuming
sulfuric
acid on the plastic and used frequent water rinses. We have a plasma etcher
but
I was afraid it would take forever to get through the plastic.

Any hints and suggestions would be greatly appreciated.

Diane Ciaburri
Senior Materials Engineer
General Dynamics
100 Plastics Ave.
Pittsfield MA 01210

____________________Forward Header_____________________

I've used it often to unpackage epoxy microchips. These
epoxies are silicone/epoxy and not true epoxy. They are
typically called plastic packaged ICs. Whatever. I've
used MEC and DiMEC to do this. Some packages
had to be oxygen ion blasted open. Over time, the plasma
method has been much safer for the operator and the chip.
And it can make the removal process much faster. Plasma
is the current method of choice.

Whether the "epoxies" talked about here are the same as
those for IC packages is likely not to be.

gary g.



From daemon Thu Jun 15 20:52:41 2000



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Thu, 15 Jun 2000 16:29:08 -0400
Subject: modulus for LaAlO3

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X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58


Hello,

Does anyone know the shear modulus for LaAlO3?

Thanks

Yan Xin
=======================================
Yan Xin
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Thu Jun 15 20:52:41 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 15 Jun 2000 13:21:14 -0700
Subject: Re: Epoxy removal from ICs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The ion beam approach works well. I have not used it
recently on finer pitch ICs. With as-built feature sizes
of 2-4u, it is fine. It will stop at the passivation and leave
the Al bond wires intact. The resulting package looks like
it has a V-shaped pit in it (which it does). The extent of the
pit depends on the size of the die and if you want to blast
down to the lead frame or substrate.

I have not done this on finer pitch devices. I would be a
bit skeptical about these mostly because of the smaller
bond pads. The etching would still stop at the passivation.

There are numerous places in Silicon Valley that do this
on an outsource basis. Typical costs are about $75 per IC.
I can get some contacts for you if you'd like.

gary g.



At 06:55 AM 6/15/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 15 20:52:42 2000



From: Barbara Foster :      mme-at-map.com
Date: Thu, 15 Jun 2000 16:46:47 -0400
Subject: Impact of the Internet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MME is currently conducting research through Microscopy & Analysis
regarding the impact of the internet on microscopy and imaging facilities.
If you have not yet faxed back your responses, we'd appreciate your
participation. The questionnaire is in the center of the May issue of M&A.

Results of this survey will be reported in a Fall issue of Microscopy &
Analysis; specific information on the impact of the internet will be
presented along with data collected from other recent MME surveys and
reports from other meetings in the "Microbrew" column in the July issue of
Advanced Imaging.

Many thanks.

Best regards,
Barbara Foster
Microscopy/Marketing & Education
125 Paridon Street, Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email:mme-at-map.com

Contributing editor: Advanced Imaging "MicroBrew"



From daemon Thu Jun 15 20:52:44 2000



From: ERIC :      biology-at-ucla.edu
Date: Thu, 15 Jun 2000 14:29:48 -0700
Subject: Osmium Fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the wealth of knowledge on the Microscopy list server,

I have a question about Osmium fixation..Basically I was curious to know
if there are any references to Osmium fixation at room temperature?
Everything I have seen so far only talks about fixation for 2 hours in the
refrigerator at 4 degrees...

Are there any drawback or problems that can occur if tissues specifically
Kidney and muscle would or could have? i.e. precipitation etc... etc....

Thanks in advance,

Eric
UCLA Medical Center





From daemon Thu Jun 15 20:52:46 2000



From: DAVID I SAXON :      DISAXON-at-prodigy.net
Date: Fri, 16 Jun 2000 03:38:26 -0700
Subject: Re: epoxy removal from ICs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} We haven't had a need to unpackage 'plastic' encapsulated IC's for a while but
} may have a need to do so soon (preferrably not impairing functionality?!). I am
} told that the last person to do this here (now retired) dripped fuming sulfuric
} acid on the plastic and used frequent water rinses. We have a plasma etcher but
} I was afraid it would take forever to get through the plastic.
}
} Any hints and suggestions would be greatly appreciated.
}
} Diane Ciaburri
} Senior Materials Engineer
} General Dynamics
} 100 Plastics Ave.
} Pittsfield MA 01210

Diane,
Yes, hot fuming sulfuric and/or hot fuming nitric are used as standard
procedures for removing plastic from IC's. The process is not quite that
simple. For example, water rinses will almost certainly etch the bond pads
on the IC and thus removing connection to the outside world. Additionally,
the plastic contains fire retardants which some regions don't like being
washed down the drain. There is more detailed help through EDFAS.org (one
of ASM's branches). B&G International sells a very safe, effective etcher
which performs decapsulation automatically in minutes.

I have no association with B&G International.

David Saxon
Analytical Microscope Services
11826 Reservoir Rd. E.
Puyallup, WA 98374
253-848-7701 voice & fax
email: info-at-analyticalmicroscope.com
website: www.analyticalmicroscope.com



From daemon Thu Jun 15 20:52:49 2000



From: Robert Blystone :      rblyston-at-trinity.edu
Date: Thu, 15 Jun 2000 18:31:30 -0500
Subject: OnCor

Contents Retrieved from Microscopy Listserver Archives
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To the LIST:

I am looking for information about an analysis software package by the
name of ONCOR. Does any have an adress for the company.... which may not
exist anymore?? Thanks
Blystone in Texas

Robert V. Blystone, PH.D.
Professor of Biology
Trinity University
San Antonio, Texas 78212
rblyston-at-trinity.edu
210-999-7243 FAX 210-999-7229



From daemon Thu Jun 15 21:23:00 2000



From: tbonner :      tbonner-at-brockport.edu
Date: Thu, 15 Jun 2000 21:14:18 -0500
Subject: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Being unable to afford a digital camera for my TEM. I'm wondering if the next
best option is to get a high end scanner to scan in negatives and then print
them on a decent printer. Any advice regarding this idea and brands of
scanners and printers that are useful? Also, what image analysis systems are
user friendly?

Dr. Thomas P. Bonner
Department of Biological Sciences
SUNY at Brockport
Brockport, NY 14420




From daemon Thu Jun 15 21:23:01 2000



From: Carrie Golash :      cdg126-at-psu.edu
Date: Thu, 15 Jun 2000 21:13:34 -0500
Subject: Embedding oocytes in araldite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello all -

I'm looking for advice on embedding bovine oocytes (~100 micron
diameter).
I'd like to embed them in araldite for probing with labelled lectins as
this seems to be fairly well established in the literature. The hangup is
this: we are using fairly large plastic cassettes and are having problems
losing the oocytes within the volume of araldite. Does anyone know of a way
around this? Is there something we can pre-embed the oocytes in to make a
smaller chip that we can then embed in the larger block (that is, of
course, compatible with polymerizing / clearing the araldite? Or does
anyone know of an altogether different method for oocyte embedding that is
more effective? Thanks in advance!

--Carrie Golash

Carrie Golash
John O. Almquist Research Center
Penn State University
University Park, PA 16802
W: (814) 865-5896
H: (814) 692-7926
http://www.das.psu.edu/dbrc/dbrc.htm




From daemon Fri Jun 16 08:24:44 2000



From: D.kierst :      dlkierst-at-config.com
Date: Fri, 16 Jun 2000 00:32:14 -0400
Subject: RE: Wanted Atomic Force Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our materials science microscopy lab is in need of a used atomic force
microscope. No specific model or make. All reasonable offers will be
considered. Please respond directly to Don Kierstead at or call
330-794-6600. Any help in this effort would be greatly appreciated.



From daemon Fri Jun 16 08:24:45 2000



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 16 Jun 2000 00:53:53 -0400
Subject: Re: epoxy removal from ICs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps it's time to comment on this thread.

Dichloromethane and dimethylformamide are relatively effective disrupters of most
epoxies but their action is accompanied by great swelling because the polymer becomes
engorged with the liquid before any significant solvation takes place. This will
destroy wire bonds on an IC.

Fuming (essentially anhydrous) sulfuric acid acts by the completely different process
of sulfonating reactive groups that remain on the polymer. The depolymerized and
sulfonated byproducts are quite soluble not only in the acid but usually in water as
well. The worst thing that you could do in this relatively straightforward process
is to wash with water at intervals because this would initiate almost instantaneous
corrosion. It would be advisable for a chemist, as someone trained in the handling
of reactive materials, to carry this out or at least to establish procedures and
train others with less experience. The action of sulfuric acid in this regard is
quite different than that of nitric. Nearly anhydrous nitric acid (completely
anhydrous is extremely difficult to prepare) is a very powerful oxidizer and could
lead to unstable, dangerous byproducts whereas the sulfonates resulting from the
sulfuric acid reaction are relatively stable. Water must, of course, be prevented
from splashing into any concentrated acid, especially sulfuric.

A very strong acid such as sulfuric behaves completely differently in the absence of
water. Since most acids are highly hygroscopic and are sold as water solutions, most
people do not observe this other side of their behavior. Without water to create an
ionized electrolyte, corrosion of metals will not take place. I have de-encapsulated
ICs for failure analysis in 200 degree sulfuric acid and been able to operate the IC
without replacing the .001" aluminum wirebonds that it came with. I recall one
instance where our company built prototype hybrid microelectronic circuits out of
such de-encapsulated ICs when their supplier was late getting a new design on the
market and the only ones available were already encapsulated.

The key is to realize that water must be excluded until the sulfonating acid has been
completely rinsed away by a non-aqueous liquid. As Mr. Saxon said, there are simple
and safe devices available for doing this operation. However, with proper care and
protective gear it can be done in a beaker on a hot plate in a fume hood. A few ml.s
of sulfuric acid are heated to drive off water until heavy vapors are observed over
the liquid (which may darken during heating due to trace impurities). The IC is
carefully lowered into the hot acid and a vigorous reaction ensues with the epoxy
almost instantly washing into the solution. After a few seconds the IC is then
quickly lifted out and held over a receiving vessel and flooded with a stream of
ethanol. Only after this is a final rinse in deionized water carried out, followed
by fresh electronic grade ethanol and forced drying in warm air.

The ready made devices which carry out the operation are typically a small bowl with
a hinged lid from which air is withdrawn by a gentle vacuum. An inert metal feeder
tube leads from a heated reservoir for the sulfuric acid and passes through the wall
of the bowl to a position where the encapsulated device is secured. When the lid is
closed and the slight vacuum applied, the hot acid is pulled into the bowl over the
device. It is somewhat self-limiting in that, if the lid is opened, there is no
driving force to bring more acid into the container. Naturally, the vacuum source
needs to be protected by a trap and all waste products properly handled no matter how
the procedure is carried out.

John Twilley
Conservation Scientist
(formerly, Manager of the Reliability Analysis Center,
Teledyne Microelectronics)

DAVID I SAXON wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} }
} } We haven't had a need to unpackage 'plastic' encapsulated IC's for a while but
} } may have a need to do so soon (preferrably not impairing functionality?!). I am
} } told that the last person to do this here (now retired) dripped fuming sulfuric
} } acid on the plastic and used frequent water rinses. We have a plasma etcher but
} } I was afraid it would take forever to get through the plastic.
} }
} } Any hints and suggestions would be greatly appreciated.
} }
} } Diane Ciaburri
} } Senior Materials Engineer
} } General Dynamics
} } 100 Plastics Ave.
} } Pittsfield MA 01210
}
} Diane,
} Yes, hot fuming sulfuric and/or hot fuming nitric are used as standard
} procedures for removing plastic from IC's. The process is not quite that
} simple. For example, water rinses will almost certainly etch the bond pads
} on the IC and thus removing connection to the outside world. Additionally,
} the plastic contains fire retardants which some regions don't like being
} washed down the drain. There is more detailed help through EDFAS.org (one
} of ASM's branches). B&G International sells a very safe, effective etcher
} which performs decapsulation automatically in minutes.
}
} I have no association with B&G International.
}
} David Saxon
} Analytical Microscope Services
} 11826 Reservoir Rd. E.
} Puyallup, WA 98374
} 253-848-7701 voice & fax
} email: info-at-analyticalmicroscope.com
} website: www.analyticalmicroscope.com





From daemon Fri Jun 16 08:24:57 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 16 Jun 2000 10:33:15 +0100
Subject: Fractures in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

We often work with clients who wish to look inside materials. Firstly the
SEM is very clever it will tell you if a material is cut with a blade or a
knife or scissors!

The only way to see the true internal structure of a material is to fracture
it. Drop the material into LN2 wait until the bubbles stop and then take it
out and using heavy duty tweezers crack it.

If a material (like hair and some polymer fibres) will not crack you need to
support them in some way to make them crack. We use a water based carbon
solution and two SEM stubs. Glue the two stubs together with the water
soluble adhesive (try Spi) and then drill two or three small holes through
the stubs (about 1mm diameter). Glue the hairs together with the carbon
solution and pass then through the holes (messy). When all is dry plunge
into LN2. Tap a blade between the two stubs and ALL the material should
fracture.

Alternatively, take a fine bore drinking straw and pass the hairs plus
carbon solution into the straw. Wait until dry, dump in LN2 and flex the
straw to crack it and its contents.

Such fractures of layered materials (e.g. paints) will be best viewed in BSE
each "phase" will either be of a different contrast or fracture in a
different way. Great fun, try it?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Fri Jun 16 08:24:58 2000



From: veys-at-bota.ucl.ac.be (Pascal Veys)
Date: Fri, 16 Jun 2000 10:50:06 +0100
Subject: search for Dr. ND Hallam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I want to contact by Email Prof. N.D. Hallam (formerly at Melbourne and LA
Tobe - Australia)
Does anybody have his contact adress
Thanks to all in advance
Pascal

""""""""'
( O)(o )
--------------------0000--------------0000----------
Pascal VEYS
Laboratory of Plant Biology
Catholic University of Louvain
Place Croix du Sud 5 (bte 14)
B 1348 Louvain-la-Neuve
Belgium
Phone : 0032 10473004
Fax : 0032 10473471
Email : Veys-at-bota.ucl.ac.be
---------------------ooooO-----------Ooooo--------
( ) (_)(_) ( )
) ( ) (
(_) (_)




From daemon Fri Jun 16 08:24:59 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Fri, 16 Jun 2000 11:44:07 +0100
Subject: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All


I«m thinking in buying a saphire knife for ultramicrotomy since they seem to
be less expensive than the traditional diamond ones. I need to cut thin
sections of sponges that are difficul to cut with glass knifes. Does anyone
have experience with these knifes? How do they compare with diamond in terms
of cutting properties and durability?


Thanks

Dr. A.P. Alves de Matos
Dental Medical School
Lisbon



From daemon Fri Jun 16 08:25:01 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 16 Jun 2000 08:17:39 -0500
Subject: Embedding oocytes in araldite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many of our customers are using Agfa Duoscan scanners with excellent
results. These are "flatbed" type scanners but handle films in a separate
drawer, similar to a negative carrier in an enlarger. The advantage of this
system is not scanning through glass, eliminating the chance of Newton
Rings.
In addition, the Duoscan line offers high optical resolutions(up to
2500x2500ppi)and high dynamic ranges.
The Umax Powerlook III is also an excellent scanner where budgets may be
limited. It has 1200x2400 optical resolution and is a traditional "flatbed"
design. Also new is the Linocolor 1400 with 1200x2400 resolution with a
letter size scan bed.

Choosing a printer is more difficult, depending on your output needs. High
end photographic printers such as the Fuji Pictrography or dyesub printers
from Kodak and Sony offer top quality output but at a high price for both
hardware and cost per print. For publication quality prints, these are the
best.
Ink jet printers continue to improve in image quality, and more
importantly, long term image stability. The cost of these printers is very
low although they are very slow, and still somewhat costly per print when
used with the higher quality print materials. Most inkjets are also better
at producing color prints than monochrome prints.
Another favorite of ours is the Tektronix Phaser 850. This high quality
plain paper printer uses a unique Solid Ink technology. Ink is supplied not
in a liquid form but a solid blocks. Cost per print is very low and black
ink is free for the life of the printer.
The Phaser 850 will also handle any "office" type output such as letters
and reports with the advantage of integrating images into pages instead of
attaching all photos at the end.

George Laing
National Graphic Supply


-----Original Message-----
} From: tbonner [mailto:tbonner-at-brockport.edu]


Carrie,

We are currently working on a project involving blastocysts and since we
aren't osmicating the samples, we also have the problem of seeing them in
the resin. Embedding them in agar helps somewhat. Even though it's also
relatively transparent, the larger size of the agar chunk makes it easier to
see.

We perform our primary fixation, then buffer washes, then make a 2% agar
solution on the hot plate. When the agar cools down enough to be quite warm
to the touch (but before the gelling stage), we pipette our cells into it on
a microscope slide or cover slip, then put it into the fridge to harden. It
hardens almost immediately. Then we cut the piece of agar with the sample
into a tiny cube and continue processing it normally.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Carrie Golash [mailto:cdg126-at-psu.edu]
Sent: Thursday, June 15, 2000 9:14 PM
To: Microscopy-at-sparc5.microscopy.com



Hello all -

I'm looking for advice on embedding bovine oocytes (~100 micron
diameter).
I'd like to embed them in araldite for probing with labelled lectins as
this seems to be fairly well established in the literature. The hangup is
this: we are using fairly large plastic cassettes and are having problems
losing the oocytes within the volume of araldite. Does anyone know of a way
around this? Is there something we can pre-embed the oocytes in to make a
smaller chip that we can then embed in the larger block (that is, of
course, compatible with polymerizing / clearing the araldite? Or does
anyone know of an altogether different method for oocyte embedding that is
more effective? Thanks in advance!

--Carrie Golash

Carrie Golash
John O. Almquist Research Center
Penn State University
University Park, PA 16802
W: (814) 865-5896
H: (814) 692-7926
http://www.das.psu.edu/dbrc/dbrc.htm




From daemon Fri Jun 16 08:35:23 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 16 Jun 2000 08:38:15 -0400
Subject: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:44 AM +0100 6/16/0, A.P. Alves de Matos wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************
I had bought a saphire knife, years ago (early 1980's). Our lab bought it
with the idea that it was a good half-way stop for a new tech who needed
something better than glass. It had certain drawbacks....the edge seemed
to collect debris and was more difficult to clean than a diamond, and of
course it wore faster too. she used it for a while (6 months?) and then
we were able to buy another diamond knife.

I haven't tried a saphire knife since, although I am partial to them in
jewelry!

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 16 17:59:08 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 16 Jun 2000 09:58:04 -0400
Subject: Re: Embedding oocytes in araldite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When we were working with porcine oocytes it was necessary to
handle each individually so we enrobed them in agarose inside a cell of
nylon net of a dark color, using a dissecting microscope. We saved enough
of the excess nylon net to use as a "handle" to pick up the sample and
moved it from solution to solution. This should be done after fixation,
since glut fixed agarose is sometimes a problem. We also used the low temp
gelling agarose, so that we had time to work. Then put it in the frig to
solidify. It will then remain solid at room temp.
You might get better lectin labeling using one of the acrylic
resins rather than an epoxy

At 09:13 PM 06/15/2000 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Fri Jun 16 17:59:09 2000



From: Alan Bright :      bright-at-dial.pipex.com
Date: Fri, 16 Jun 2000 15:00:37 +0100
Subject: SEM, How to interpret a fractured adhesive film

Contents Retrieved from Microscopy Listserver Archives
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Dear Ric,

I see no reason why this type of cross sectional view cannot be achieved by
sectioning with a cryostat, this is the type of use our cryostats are
supplied for. If you would like more information please get back to me, I
would be happy to section some samples for you to inspect.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++=

-----Original Message-----
} From: Smartech [mailto:smartech-at-javanet.com]
Sent: 15 June 2000 15:03
To: To all on the list


I needed to have a cross sectional view into an adhesive layer so that I
could count the layers, if possible. Since the adhesive acts like a highly
viscous liquid, polishing it is out of the question. Instead, I submerged
the film into liquid Nitrogen and cut it quickly w/ a scissors. The sound
of the cut was more like a breaking sound. When I examined the cross
sectional surface I observed a brain like surface. There were columns with
a highly consistent diameter and orientation. It looked crystalline. There
was zero evidence of material smearing that one would expect if one cut a
material. Is the proper interpretation that these columns existed before
the fracture and that the fracture occurred along the boundaries? Or is
the cross sectional surface generated by a rippled distortion of an highly
viscous liquid? The regularity of the surface seems to make the latter
interpretation unlikely.

I would be interested in any opinions on the interpretation of the image or
alternative means of cross sectioning the adhesive layer.

I could e-mail an image to anyone who is interested.

Thanks

Ric




From daemon Fri Jun 16 17:59:10 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Fri, 16 Jun 2000 09:35:03 -0500
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
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I'll ring in & say yes. I am quite happy with this combination. You get the
digitized images with a much large field of view. Photo quality ink jets are cheap
& in general do well. You will always find extremist in on the subjects of the
infinitely best scanner & printer but here is what I bought for {9K$.
AGFA Duoscan T2500 ~$4500
500MHz PC with 1/2 Gig memory & 19" hi res monitor, CD writer ~2.5K$
Epson Stylus 870 ~$300
Photo Shop, Fovea 1.0 IP software {1K$ with student ver. of PS
Misc. supplies some $

For a MacPerson, my understanding is that in terms of image processing speed
the Macs are 2-4x faster that PC but I don't have any benchmarks on the latest
generations.

If your printing a lot, the ink jets will drain cartridges pretty quick. Down
the road I will probably pick up one of the wax printers. They cost something like
3k$ but I think it is Tektronix that offers to supply all the black wax you can
user for life, they are pretty fast & no secret papers are required (much cheap
per BW page).

Just my thoughts before coffee.

Bruce Brinson

disclaimer... no financial interest in any companies mentioned.

tbonner wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Being unable to afford a digital camera for my TEM. I'm wondering if the next
} best option is to get a high end scanner to scan in negatives and then print
} them on a decent printer. Any advice regarding this idea and brands of
} scanners and printers that are useful? Also, what image analysis systems are
} user friendly?
}
} Dr. Thomas P. Bonner
} Department of Biological Sciences
} SUNY at Brockport
} Brockport, NY 14420



From daemon Fri Jun 16 17:59:10 2000



From: kklos-at-mail.mse.ufl.edu
Date: Fri, 16 Jun 2000 10:36:03 -0400
Subject: Stuers Jet Polisher

Contents Retrieved from Microscopy Listserver Archives
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I have been trying to replace the photocell on the Jetpolisher that I have
been using. It is about 15 years old and is made by Struers. The Model is
a Tenupol and the power supply is type is Polipower. Struers no longer
makes replacement parts for these units. If anyone has any information
concerning the photocells of this model (who I might contact to replace it
or the sensitivity of the photocell) it would be greatly appreciated.
Regards
Kevin



From daemon Fri Jun 16 17:59:11 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 16 Jun 2000 07:37:38 -0700
Subject: RE: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thomas writes ...

} Being unable to afford a digital camera for my TEM. I'm
} wondering if the next best option is to get a high end
} scanner to scan in negatives and then print
} them on a decent printer. ...

The next best option would be a 4x5 film scanner (~US$4k). The
problem with typical film scanners is their anticipated dynamic range
for photographic film, which is where TEM digital capture excels. I
suggest you take a representative film and evaluate the Polaroid "4x5
Ultra". Althought I'm unfamiliar with this particular 4x5 scanner, it
is the only one (I'm aware of) which is purported to scan an OD better
than 3.5 (approximately 14 f/stops ... 4 f/stops per OD unit ...
correct me if I'm wrong).
Less expensive (~US$1.2k) would be a flat bed scanner designed for
transparencies as well as hardcopy. This additional feature could be
a "drawer" for film, or a optional "lid" which provides a lamp from
above.

=shAf=



From daemon Fri Jun 16 17:59:13 2000



From: EBMet-at-aol.com
Date: Fri, 16 Jun 2000 11:34:47 EDT
Subject: re: Cross-section Preparation of Magnetic Recording Tape

Contents Retrieved from Microscopy Listserver Archives
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To All:

I need tips, descriptions, references regarding the preparation of
cross-section specimens of the magnetic layer of magnetic recording tape for
high magnfication SEM and conventional TEM observation. Thanks.

Elliot Brown
EBMET-at-AOL.COM



From daemon Fri Jun 16 17:59:14 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Fri, 16 Jun 2000 11:48:57 -0400
Subject: RE: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Actually the printing part has recently gotten much
easier. ElectroImage (http://www.electroimage.com) is offering new
technology that lets you print real grey scale images on simple inkjet
printers. They have grey inks and new printer drivers.

Bill Miller



At 08:47 AM 6/16/00 -0700, George Laing wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jun 16 17:59:15 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 16 Jun 2000 12:11:08 -0400
Subject: re: Cross-section Preparation of Magnetic Recording Tape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Elliot:

Off hand I know I have information on the cross sectioning of hard disk
media using the Tripod Polisherš. I'm not sure if I have anything on
magnetic recording tape. If you send me your mailing address, I'll send
you whatever I can find that comes close.

Best regards-

David
Writing at 8:46:59 AM on 06/16/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by INTERNET:"EBMet-at-aol.com"-at-sparc5.microscopy.com
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To All:

I need tips, descriptions, references regarding the preparation of
cross-section specimens of the magnetic layer of magnetic recording tape
for
high magnfication SEM and conventional TEM observation. Thanks.

Elliot Brown
EBMET-at-AOL.COM

{



From daemon Fri Jun 16 17:59:15 2000



From: Edward Haller :      ehaller-at-hsc.usf.edu
Date: Fri, 16 Jun 2000 12:24:30 -0400
Subject: Osmium fix

Contents Retrieved from Microscopy Listserver Archives
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In response to Eric's question, I run a diagnostic Pathology lab at the
University of South Florida, and have a one day processing schedule for
kidney biopsies that calls for osmication in 1% buffered osmium at room
temperature for 30 minutes. I have found that for tissue pieces in the
order of 1/2 millimeter thick in one dimension the fixation is fine, and
is equivalent to our routine processing osmium fixation of one hour at 4
degrees. I haven't tried this on muscle biopsies, but if they meet the
thickness criteria they should be O.K. too,. Just make sure to rinse
these extensively (3x 10 minutes, perhaps) in buffer to remove the
excess osmium from the muscle tissue, as fluids enter and leave muscle
slower because of the extensive connective tissue sheaths around the myocytes.
Overosmication is a definite possibility, with subsequent tissue
brittleness, if tissue is left in osmium too long, no matter what the
temperature. If the tissue is too thick, uneven osmication can occur,
where the outside of the tissue is well fixed and a fixation gradient is
set up with poor fixation towards the center of the tissue. I observed
this happening at a renal lab in Pittsburgh where I used to work. Our
unstained thick sections were darker at the periphery than in the
center. Another sign of this problem is lack of specimen contrast at the
center of thin sections.
So, Eric, as far as my experience goes, it is possible to start with 4
degree, buffered osmium, and to osmicate at room temperature for a half
of an hour and get results equal to those from osmication at 4 degrees
for one hour if your tissue is sufficiently thin in at least one
dimension. I haven't noticed any precipitation problems with this
technique, nor does the osmium discolor during fixation. Our lab has
been using this technique for several years now. Take care! Ed Haller,
U.S.F. Pathology



From daemon Fri Jun 16 17:59:16 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 16 Jun 2000 12:33:25 -0400
Subject: Struers Jet Polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kevin:

You may want to ask Struers for a users list. There may be someone out
there with an older unit that is no longer being used who may be willing to
give it to you for spare parts. If they can't give you a list, let me know
- I think I can dig up an old list I put together of some previous Tenupol
users you may be able to contact. If that doesn't work, you may want to
consider upgrading to a South Bay Technology Model 550D Jet Polisher. If
you have an interest in getting more information on that option, please
contact me and I'll send you information.

Best regards-

David
Writing at 8:56:57 AM on 06/16/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"kklos-at-mail.mse.ufl.edu"-at-sparc5.microscopy.com
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



I have been trying to replace the photocell on the Jetpolisher that I have
been using. It is about 15 years old and is made by Struers. The Model is
a Tenupol and the power supply is type is Polipower. Struers no longer
makes replacement parts for these units. If anyone has any information
concerning the photocells of this model (who I might contact to replace it
or the sensitivity of the photocell) it would be greatly appreciated.
Regards
Kevin
{



From daemon Fri Jun 16 17:59:16 2000



From: Edward Haller :      ehaller-at-hsc.usf.edu
Date: Fri, 16 Jun 2000 12:58:34 -0400
Subject: Immunofluorescence using plastic coverslips

Contents Retrieved from Microscopy Listserver Archives
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To the person asking about the possibility of doing immunofluorescence
microscopy on cells grown on plastic coverslips, someone has published a
technique in BioTechniques that I saved in case I needed it. Volume24,
number 6, pages 910-914, 1998 is the article titled "Mounting technique
allows observation of immuno-labeled cells on plastic coverslips".
The basic technique from M. F. Donohue et al involves using Thermanox
coverslips on which cells are grown and immunolabeled. Following
labeling, this group uses a drop of aqueous mounting medium to mount the
side of the coverslip without cells on it to a glass slide. On top of
this, the group then mounted a regular glass coverslip with an
additional drop of aqueous mountant, and then could do their microscopy.
The authors state that the inherent strong autofluorescence is greatly
reduced by this technique, and the problem with the plastic not
transmitting light well is overcome. Although I haven't tried the
technique yet, it sounds like a simple fix for a sticky problem. I hope
this is of help to you! Ed Haller, U.S.F. Pathology



From daemon Fri Jun 16 17:59:17 2000



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Fri, 16 Jun 2000 11:50:10 -0500
Subject: Tissue Processors

Contents Retrieved from Microscopy Listserver Archives
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Hi,
We are currently in the market for a tissue processor for electron
microscopy. It will mainly be used for biolgical specimens (some quite
small). Although I have considerable experience with the processor sold
by RMC (Ventana), I know virtually nothing about the Lynx (now being
sold by EMS, I think). Any information on the advantages or
disadvantages of either model (or any other one that might be out there)
would be appreciated. Offline replies are welcome.

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75390
Email: tom.januszewski-at-email.swmed.edu



From daemon Fri Jun 16 17:59:17 2000



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 16 Jun 2000 10:22:28 +0100
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
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We are using the Agfa T2500 to scan TEM and SEM negatives, which also
gives us the ability to scan prints. The 1200 dpi is sufficient for
most needs, with 2500 dpi getting used less often and mostly with low
mag images. It is currently connected to a 233 MHz Mac G3/160 Mb RAM,
being replaced with a 400 MHz G4/320 Mb. The Agfa is driven by either a
stand alone app. or through a plug-in that runs under most software,
such as Photoshop or Object Image. You will want a lot RAM and drive
space, CDR, Ord, Jaz or DVD-RAM drives. Zip drives fill far too quickly.


Photoshop is used for publication images, although some users prefer
Canvas. Most of our image analysis uses the Object Image enhanced
version of NIH Image. It is very easy to use. I've less experience with
Image/J, but it is quickly adding capabilities and will display } 8 bits,
whereas Object Image will process 16 but only displays 8 bits. Printing
is either to an Epson 850, 3000, or our venerable Phaser IIsdx. BTW,
Tektronix sold its printer division to Xerox.

tbonner wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Being unable to afford a digital camera for my TEM. I'm wondering if the next
} best option is to get a high end scanner to scan in negatives and then print
} them on a decent printer. Any advice regarding this idea and brands of
} scanners and printers that are useful? Also, what image analysis systems are
} user friendly?
}
} Dr. Thomas P. Bonner
} Department of Biological Sciences
} SUNY at Brockport
} Brockport, NY 14420

--

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156
(206) 616-1828 fax
***********************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
***********************************************************



From daemon Fri Jun 16 17:59:21 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 16 Jun 2000 11:47:54 -0700
Subject: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I«m thinking in buying a saphire knife for ultramicrotomy since they seem to
} be less expensive than the traditional diamond ones. I need to cut thin
} sections of sponges that are difficul to cut with glass knifes. Does anyone
} have experience with these knifes? How do they compare with diamond in terms
} of cutting properties and durability?
}
} Dr. A.P. Alves de Matos
} Dental Medical School
} Lisbon

Unfortunately, you get what you pay for. Sapphire does NOT have the
durability of diamond and will be damaged by the sponge spicules. And, to
my knowldge, they can't be resharpened.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Jun 16 17:59:24 2000



From: Jon Ekman :      jekman-at-csd.uwm.edu
Date: Fri, 16 Jun 2000 03:18:56 -0500
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It could be a solution if you mainly want an alternative to silver prints,
or to acquire images for Powerpoint presentations. However, many
of the really useful features of a digital camera on a TEM are
unavailable if you adopt this approach, namely instant verification of
image capture, greyscale expansion, image averaging, online
analysis, and many more. Also, you still need to allocate some
space to a darkroom.

I looked at large-format transparency scanners a couple of years
ago, when there was little of this kind in the market, and concluded
that their combinations of bit depth, pixels per inch and sensitivity
and dynamic range at the high-density end of the negative (i.e.
highlight detail) was close to what was required if the objective was
merely to obtain publication quality images from a large proportion
of negative area (these images require optimised contrast and
crispness, but being small do not demand much resolution), but
really inadequate for scanning of image details (organelles,
molecules), for dense exposures and highlights, and for high
contrast subjects like replicas. Most of these scanners appeared to
be optimised for scanning positive images (large format colour
transparencies) where discrimination of detail in the extreme
shadows is not top priority. However, this becomes a major
shortcoming when dealing with negatives.

I would certainly like to know whether anyone feels that there is an
adequate solution available today.

I don't think user friendliness is the most useful criterion for
discriminating between image analysis packages. This is in any
case a fairly subjective property, depending very considerably on
the computer - literacy of the user. Most IA packages (analySIS,
Optimas, etc) are GUI-based systems, and therefore are reasonably
intuitive. One of the features that differentiates them is the balance
between the provision of off-the-peg analysis solutions and
programmability. The range of tasks demanded of an IA package is
potentially so great that there is little alternative but to evaluate them
and see if they suit your needs. However, I warn you that your
needs are likely to evolve. What seems like a simple and user-
friendly solution today will probably feel like a very limited and
inflexible one tomorrow if it has insufficient functionality and
programmability, and these things unavoidably add complexity.

Chris Jeffree

Date sent: Thu, 15 Jun 2000 21:14:18 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: tbonner {tbonner-at-brockport.edu}



tbonner said:
} Being unable to afford a digital camera for my TEM. I'm wondering
} if the next best option is to get a high end scanner to scan in
} negatives and then print them on a decent printer.

Using a flat bed scanner over a digital camera for TEM images is
definately your best option budget wise. We use an Agfa Duoscan
scanner with great success. it is very versitile, we use it to scan
gels, scan TEM negatives and scan old prints. It can scan slides for
powerpoint quality presentations also. So check with Agfa to see
their latest lineup.

For printers inkjets work great when combined with photoquality
papers. Any (Epson HP & Cannon) 300dpi or higher color ink jet will
give decent images suitable for posters. For publications dye
sublimation printers work well but get one that uses a cartridge (one
piece) to replace empty media. For proofing look for laser printers
that are at least 1200dpi with extra ram (64meg on the printer is a
nice number to start with) and large toner cartridges, graphic
images burn a lot of toner. Look for a printer that will print alot
before replacement of the toner.

For software, Photoshop is a good choice. Before you spend alot on
a venders image analysis package try some of the freeware out
there like NIH image and Image J both from the NIH website. If you
can't get the free stuff to work, then spend the extra money. We use
Image J here and it works well.

For computers (Apple or PC) consider one scanner with a SCSI
interface with need a SCSI card (avoid parallel port and universal
serial bus (USB) scanners unless you like coffee breaks). So you
need one computer to hook up to the scanner but Ideally you would
also have three printers (inkjet, Laser & Dye sub or comparable) But
Inkjets are cheap make your users get one and maintain it. I bet
somewhere in your department is a networked laser printer so print
to a networked printer elsewhere. Keep the high end printer (Dye
sub/thermal printer close by) Invest in removable media that others
can use like a CD-r (HP or Plextor) and a zip drive at the bare
minimum.


Hope this helps
Jon Ekman
Associate Research Specialist
Deptartment of Biological Sciences
University of Wisconsin-Milwaukee
phone W:414.229.6471
Web1 http://www.graffitimasters.com
Web2 http://www.uwm.edu/~jekman



From daemon Fri Jun 16 18:19:28 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Fri, 16 Jun 2000 15:48:24 -0400
Subject: RE: Cross-section Preparation of Magnetic Recording Tape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Embed it, Elliot and diamond knife section it in an ultramicrotome, using
the block face for the FE-SEM examination and the ultrathin sections (20-200
nm thick) for examination in the TEM. A good reference is Ho et al,
Specimen Preparation for TEM of Materials, Mat. Res. Soc. Symp. Proc., vol.
115, pp. 149-154 (MRS, Pittsburgh, 1988). You may have to search around for
someone who can do this for you, but it is far and away the best technique
for your needs.

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


----------
From: "EBMet-at-aol.com"-at-sparc5.microscopy.com
[SMTP:"EBMet-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, June 16, 2000 11:35 AM
To: microscopy-at-sparc5.microscopy.com
Subject: re: Cross-section Preparation of Magnetic Recording Tape


------------------------------------------------------------------------
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To All:

I need tips, descriptions, references regarding the preparation of
cross-section specimens of the magnetic layer of magnetic recording
tape for
high magnfication SEM and conventional TEM observation. Thanks.

Elliot Brown
EBMET-at-AOL.COM



From daemon Fri Jun 16 23:04:06 2000



From: Gen Pei :      gp35-at-cornell.edu
Date: Fri, 16 Jun 2000 22:57:08 -0400
Subject: How to know the ion milling rate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI,

I am ion milling gold. nearly 100 nm. I want to know how long it
takes to make it thin enough to be transparent under TEM. Thanks.

Gen
******************************************************************
Gen Pei
Department of Materials Science and Engineering
Cornell University
328 Thurston Hall
tele: (607)255-5177
fax:(607)255-2365
gp35-at-cornell.edu

******************************************************************



From daemon Sat Jun 17 09:24:45 2000



From: cgarber-at-2spi.com
Date: Sat, 17 Jun 2000 06:52:25 -0700
Subject: RE: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



For your information:

a) So far as I know they are not made any more and have not been
made for at least ten years, and

b) The economics have changed drastically from twenty years ago
when the sapphire knife did enjoy a bit of popularity. In real
terms, diamond knives, now because of the competition from Microstar
have drop significantly from what they once were, perhaps 50%,
so whatever pricing advantage there was at one time, did not
exist any more. So the Japanese company that made them discontinued
their production. It was called "Saphatome" or something like
that. Ted Pella would probably know their history, perhaps better
than I do.

Also, because of your interest in education, take a look at www.microscopy-advantage.com
. Tell me what you think. Attendees at the coming meetings
of APEM, EUREM and MSA will automatically receive a CD in their
registration materials. If you would like a copy, send me your
UPS address and I will make sure that one gets sent to you. But
it will "work" exactly as if you were on line.
--- Original Message ---
Caroline Schooley {schooley-at-mcn.org} Wrote on
Fri, 16 Jun 2000 11:47:54 -0700
------------------
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America



} IŽm thinking in buying a saphire knife for ultramicrotomy since
they seem to
} be less expensive than the traditional diamond ones. I need
to cut thin
} sections of sponges that are difficul to cut with glass knifes.
Does anyone
} have experience with these knifes? How do they compare with
diamond in terms
} of cutting properties and durability?
}
} Dr. A.P. Alves de Matos
} Dental Medical School
} Lisbon

Unfortunately, you get what you pay for. Sapphire does NOT have
the
durability of diamond and will be damaged by the sponge spicules.
And, to
my knowldge, they can't be resharpened.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





-----
Sent using MailStart.com ( http://MailStart.Com/welcome.html )
The FREE way to access your mailbox via any web browser, anywhere!



From daemon Sat Jun 17 10:23:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 17 Jun 2000 08:11:45 -0700
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:15 PM 6/16/00, you wrote:

} It could be a solution if you mainly want an alternative to silver prints,
} or to acquire images for Powerpoint presentations. However, many
} of the really useful features of a digital camera on a TEM are
} unavailable if you adopt this approach, namely instant verification of
} image capture, greyscale expansion, image averaging, online
} analysis, and many more. Also, you still need to allocate some
} space to a darkroom.

Why not just outsource the processing of the film? Depending
on where one resides/operates, there are typically numerous
professional and non-professional labs which will do same day
development of b/w film. I do this for 4x5 cut sheet film and
120/220 roll film from a regular camera and from the SEM recording
camera. Unless there is some overriding need or requirement for
an on-site darkroom, why not just send the film out whenever
it is needed? I could see the rationale for an on-site facility if
the TEM was producing hundreds of negs per day or perhaps
per week. Then it is a make-buy decision regarding in-house
or out-house processing.

If one is concerned about whether a shot will turn out (instant
verification), just shoot a couple more sheets or frames bracketed
around the "optimum/normal" exposure time. The cost of the
film and processing is way too low to justify a high cost digicam
for TEM. SEM imaging is of course a totally different
matter.

I find that grey scale expansion is not the sole domain of the
digicam. In a neg, additional information is there--but typically
the eye cannot see it. This is where image analysis and image
processing programs are very beneficial.


} I looked at large-format transparency scanners a couple of years
} ago, when there was little of this kind in the market, and concluded
} that their combinations of bit depth, pixels per inch and sensitivity
} and dynamic range at the high-density end of the negative (i.e.
} highlight detail) was close to what was required if the objective was
} merely to obtain publication quality images from a large proportion
} of negative area (these images require optimised contrast and
} crispness, but being small do not demand much resolution), but
} really inadequate for scanning of image details (organelles,
} molecules), for dense exposures and highlights, and for high
} contrast subjects like replicas. Most of these scanners appeared to
} be optimised for scanning positive images (large format colour
} transparencies) where discrimination of detail in the extreme
} shadows is not top priority. However, this becomes a major
} shortcoming when dealing with negatives.

Discrimination of detail in shadows is a major concern for
users of transparencies. This is why they seek high D rated
scanners. I typically scan negative and transparencies as
transmitted RGB or greyscale. This is because I find that the
scanner picks up more detail across the whole image when
scanned as a tranny.

} [snip]

} and see if they suit your needs. However, I warn you that your
} needs are likely to evolve. What seems like a simple and user-
} friendly solution today will probably feel like a very limited and
} inflexible one tomorrow if it has insufficient functionality and
} programmability, and these things unavoidably add complexity.
}
} Chris Jeffree

I agree that needs may evolve. That means that when making
the initial purchase of an image analysis program, it should
be flexible enough to allow custom augmentation. Most of
the higher end ones do. But it may turn out that one program
alone is not as good as two different programs--each being
good at different aspects of image analysis.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sat Jun 17 10:53:16 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 17 Jun 2000 11:50:47 -0500
Subject: Magnetic recording tape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tom Malis wrote:
======================================================
Embed it, Elliot and diamond knife section it in an
ultramicrotome, using
the block face for the FE-SEM examination and the ultrathin
sections (20-200
nm thick) for examination in the TEM. A good reference is Ho et
al,
Specimen Preparation for TEM of Materials, Mat. Res. Soc. Symp.
Proc., vol.
115, pp. 149-154 (MRS, Pittsburgh, 1988). You may have to search
around for
someone who can do this for you, but it is far and away the best
technique
for your needs.
======================================================

This has been our experience too, however we add the following to the
preparation protocols:

a) We coat one side of the recording tape with, say, Pt, the other side
with Al, because once in the TEM, it is important to validate that i]
nothing has fractured off during the ultramicrotomy and ii] you can keep
straight which side is which for the asymmetric cross-section. If the two
metallization lines are present in the TEM, with embedding resin on the
other side, you can be certain you are seeing the entire cross-section. If
one is missing, you might not have the entire cross-section.

b) For looking at the "faced-off-piece", we suggest an ever so slight
amount of oxygen plasma etching, in order to bring out a bit more contrast
between the ferrite or other inorganics from the matrix polymer. The
inorganics stand up like little "mesas" in the desert, giving greatly
enhanced contrast. Since you now have an element of three dimensional
nature to the same, you can gain some insight into orientation, something
that would not otherwise be possible

Disclaimer: If you are looking for someone to do this kind of work, look no
further, we have been doing this kind of sample preparation for clients on
a contract basis since the early 1970's! Our own Plasma Prep II plasma
etcher would do the described etching on the faced-off-piece after about 120
seconds of exposure.

Chuck


============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Jun 17 17:33:47 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 17 Jun 2000 09:10:57 -0700
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

And if you're satisfied with prints at 720x1440 dpi, Epson has just made a
major leap in ink and paper longevity; read about it at
http://www.epson.com/whatsnew/ygtsi/lightfast.html
http://www.wilhelm-research.com/ . Unfortunately, the new ink cartridge
won't fit old (as in last year's) printers. Oh well - what else might I
spend $370 on?

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Jun 17 17:33:52 2000



From: Dave Audette :      deaudette-at-yahoo.com
Date: Sat, 17 Jun 2000 13:57:45 -0700 (PDT)
Subject: job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List Recipients:
I am posting this message at the request of Joe Lester
and all correspondence should be sent to him.
Dave Audette
david.audette-at-sylvania.com

JOB OPENING
Scanning Electron Microscopist in Analytical
Laboratory ( Beverly, MA)

OSRAM Sylvania, Inc.
71 Cherry Hill Drive
Beverly MA 01915

DESCRIPTION:
Structural and elemental characterization of
materials used in incandescent,
fluorescent and discharge lamps, especially by
optical and electron
microscopy. Failure analyses of lamps and lighting
components. Technical
problem solving as a member of a team. Oral and
written communication of
results and conclusions with client population.

POSITION REQUIREMENTS:
Competence in optical and electron microscopy of
materials including EDS. An
understanding of failure analysis. Ability to work
independently and/or in a
team and to communicate effectively.

EDUCATION AND EXPERIENCE REQUIREMENTS:
B.S., or higher, in Materials Science,
Chemistry, or Physics. 2-5
years experience in SEM/EDS. Experience with lamp
components is desirable.


Please send a resume to


Dr. Joe Lester
Technical Assistance Lab
OSRAM Sylvania Inc.
71 Cherry Hill Drive
Beverly, MA 01915
e-mail: joe.lester-at-sylvania.com



__________________________________________________
Do You Yahoo!?
Send instant messages with Yahoo! Messenger.
http://im.yahoo.com/



From daemon Sun Jun 18 19:19:45 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 18 Jun 2000 19:04:44 -0500
Subject: Administrivia: Listserver Archives Updated.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

I had fallen behind on the Archive Updates. They are now
current through May 31, 2000.

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
Your Friendly Neighborhood SysOp.





From daemon Sun Jun 18 19:29:41 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Mon, 19 Jun 2000 10:23:25 +1000
Subject: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day All,
In my experience Sapphire Knives are an excellent replacement for glass
knives when working with soft materials. Sapphires are much softer than
diamond and are more easily damaged than diamond. Sponges are NOT soft
tissue, they contain very hard inorganic salt spicules that damage both
glass and sapphire knives.
Regards
JVN

Leona Cohen-Gould wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 11:44 AM +0100 6/16/0, A.P. Alves de Matos wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear All
} }
} }
} } I«m thinking in buying a saphire knife for ultramicrotomy since they seem to
} } be less expensive than the traditional diamond ones. I need to cut thin
} } sections of sponges that are difficul to cut with glass knifes. Does anyone
} } have experience with these knifes? How do they compare with diamond in terms
} } of cutting properties and durability?
} }
} }
} } Thanks
} }
} } Dr. A.P. Alves de Matos
} } Dental Medical School
} } Lisbon
}
} *********************
} I had bought a saphire knife, years ago (early 1980's). Our lab bought it
} with the idea that it was a good half-way stop for a new tech who needed
} something better than glass. It had certain drawbacks....the edge seemed
} to collect debris and was more difficult to clean than a diamond, and of
} course it wore faster too. she used it for a while (6 months?) and then
} we were able to buy another diamond knife.
}
} I haven't tried a saphire knife since, although I am partial to them in
} jewelry!
}
} Lee
}
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Sun Jun 18 21:29:21 2000



From: XUELI ZHAO :      xzhao-at-unlserve.unl.edu
Date: Sun, 18 Jun 2000 21:10:49 -0500 (CDT)
Subject: Sight seeing while EM meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Every one:

I had a argument with my husband, he says he is going to the
Kunming for an EM meeting,

http://www.iphy.ac.cn/microsc/IKSM.html

He says there are many good scientists going which I believe with doubt.
But I think he is going for a sight seeing. There is a Chinese saying:
The mountains and waters in Guilin are the most beautiful ones under the
sky. I have asked him to bring me, he agreed but unable to get the same
airline ticket (UA fully booked). Any body is going and knows alternative
airlines, please contact me. Thanks a lot.

Xueli




From daemon Mon Jun 19 07:37:44 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 18 Jun 2000 20:55:08 -0700
Subject: Warning about DVD-RAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have two DVD-RAM drives and 15 media (Type I and II).
I have found that these are riddled with write & read errors.
Be careful when using this storage media.

My main unit is a Panasonic LF-D101 (SCSI) and
the second one is the same. The third is a Matshushita
ID unit.

Scandisk will report either many errors that are fixed or
no errors. Either way, the media/drive will write faulty
file contents.

Be careful.




From daemon Mon Jun 19 10:01:27 2000



From: ComCryLab1-at-aol.com
Date: Mon, 19 Jun 2000 10:27:57 EDT
Subject: CRYSTAL: MgAl2O4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Hao,
We have in stock (100), (110) and (111) MgAl2O4 substrates with 7 angstrom
finish ready for laser ablation or other kinds of epitaxial film deposition.
Contact me for info regarding perovskite lattice matching.
Best regards,
Mike Urbanik
www.crystalguru.com

{ { Subj: information about MgAl2O4
Date: 6/15/00 3:33:44 PM Eastern Daylight Time
From: haoli-at-glue.umd.edu (Hao Li)
To: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Hi, All:

This one is not really related to microscopy. But since I am a TEM guy, I
hope I can get some help here.

Does anybody have some information about MgAl2O4 as a substrate for
perovskite films? I know it is not often used, so I am worndering if it has
a major disadvantage so that nobody is using it. Any references would be
welcome.


Thanks a lot

Hao Li

} }



From daemon Mon Jun 19 10:01:28 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 19 Jun 2000 09:46:49 -0400
Subject: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I processed a series of pellets of yeast for a client using a glut-pfa fix,
osmium, dehydration through ethanols, and a day & a half step-wise
infiltration into Spurr's resin (1:1 with Ethanol for 4 hr, pure resin
overnight under light vacuum, then fresh for 3 more hours, then embed in
fresh). Polymerization was overnight at 60C. About half the blocks were
soft and had to be returned to the oven for a prolonged polym. (over the
weekend). I was able to get sections from each of the 10 samples, but in
the 'scope, many of these looked a bit like swiss cheese. those that were
not lacy exhibited areas where the resin pulled away from the cell coats of
the yeasts. Clearly something went wrong with the
infiltration/polymerization. I used the same batch of resin for other
things, and its fine.

Does anyone out there have experience with yeast? Any suggestions?
I'd like to give this peson some usable data!

Thanks in advance,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jun 19 18:23:25 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 19 Jun 2000 10:09:46 -0500
Subject: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Lee,

My only thought is that your infiltration/dehydration were too short, or
your 100% ethanol had absorbed too much water. My understanding is that
Spurr's is very sensitive to small quantities of water, with soft blocks and
holes being symptoms of incomplete dehydration. Sometimes we extend the
dehydration through 3 changes of 100% ETOH with molecular sieves, then on
through 2-3 changes of propylene oxide. Infiltration is usually 1:2
PO:Resin, followed by 1:1, 2:1, then a couple changes of pure resin
overnight or for 4-8 hours, then final embedding in another change of pure
resin.

I don't know if yeast is more problematic than other things in this respect,
however.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-mail.med.cornell.edu]
Sent: Monday, June 19, 2000 8:47 AM
To: Microscopy-at-sparc5.microscopy.com


Hi All,
I processed a series of pellets of yeast for a client using a glut-pfa fix,
osmium, dehydration through ethanols, and a day & a half step-wise
infiltration into Spurr's resin (1:1 with Ethanol for 4 hr, pure resin
overnight under light vacuum, then fresh for 3 more hours, then embed in
fresh). Polymerization was overnight at 60C. About half the blocks were
soft and had to be returned to the oven for a prolonged polym. (over the
weekend). I was able to get sections from each of the 10 samples, but in
the 'scope, many of these looked a bit like swiss cheese. those that were
not lacy exhibited areas where the resin pulled away from the cell coats of
the yeasts. Clearly something went wrong with the
infiltration/polymerization. I used the same batch of resin for other
things, and its fine.

Does anyone out there have experience with yeast? Any suggestions?
I'd like to give this peson some usable data!

Thanks in advance,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jun 19 18:23:27 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 19 Jun 2000 09:17:18 -0700
Subject: Re: How to know the ion milling rate

Contents Retrieved from Microscopy Listserver Archives
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Dear Gen Pei,
I have looked at gold contacts, lifted from an electronic device, that were
supposed to be 100 nm thick. I could see the structure clearly at 200 kV.
At 10:57 PM 6/16/00 -0400, you wrote:

} HI,
}
} I am ion milling gold. nearly 100 nm. I want to know how long it
} takes to make it thin enough to be transparent under TEM. Thanks.
}
} Gen

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Jun 19 18:23:29 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 19 Jun 2000 11:25:24 -0700
Subject: Formvar & 1,2 dichloroethane

Contents Retrieved from Microscopy Listserver Archives
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Hi:

I was just visited by one of our EH&S folks who wanted to know why I had
1,2 dichloroethane.

Seems they track purchases and I bought some last year to make formvar films.

1,2 dichloroethane is on their bad list as a carcinogen. He was actually
here to figure out how much might be going up the fume hood so he could
make a report to the local air quality agency. But as we talked, it seemed
like it would be better to not have the stuff around.

Anyone have experience with formvar in chloroform? I read it works but have
never tried it. According to our EH&S guys, chloroform would be better than
1,2, dichloroethane.

BTW we are in California and must abide by some pretty strict rules, it may
seem like they are going overboard, but they are just trying to do their
job.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon Jun 19 18:23:29 2000



From: Connie A Cummings :      rosscac-at-okstate.edu
Date: Mon, 19 Jun 2000 12:42:17 -0500
Subject: Sperm Processing for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{P} Good day to all on the listserver, {/P} {P}   {/P} {P} I have a person in my department who is interested in processing horse sperm for TEM.  Does anyone who does this routinely be willing to give me some pointers as to how to process them?  I've only worked with muscle and brain tissue so this is kinda new - I have processed cells from cell culture for TEM would it be the same procedure? {/P} {P}   {/P} {P} Thanks so much, {/P} {P} Connie Cummings, DVM {/P} {P} Instructor Anatomic Pathology {/P} {P} Department VBP {/P} {P} Oklahoma State University {/P} {P} {/P}

{/html}



From daemon Mon Jun 19 18:23:30 2000



From: Mike Coviello :      coviello-at-mae.uta.edu
Date: Mon, 19 Jun 2000 15:12:30 -0500
Subject: TEM-looking for a for-hire independent FIB company that does TEM

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---------- Forwarded message ----------


Hi Y'all:
We are looking for a for-hire independent FIB company that has
experience in preparing TEM cross-sections of semiconductors. Please
contact me if you do this, or know of a lab that does.
Regards,
Michael Coviello
Lab Manager
Materials Science
University of Texas at Arlington



From daemon Mon Jun 19 18:23:32 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 19 Jun 2000 16:06:34 -0400
Subject: RE: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Thanks to everyone for your helpful comments. I'm taking another stab at
it with smaller pellets, longer times and the addition of prop. ox. steps
after the ethanol.
I had pretty much decided to do all that anyway, but its nice to gets
confirmation ofone's ideas!

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jun 19 18:23:33 2000



From: Jean-Pierre Slakmon :      slakmon-at-soquelec.com
Date: Mon, 19 Jun 2000 17:56:55 -0400
Subject: TEM-looking for a for-hire independent FIB company that does

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try FIBICS in Ottawa, Canada. Contacts are Mike Phaneuf or Louise Weaver.
Their telephone number is 613-860-0861.
email: mphaneuf-at-fibics.com or lweaver-at-fibics.com

Jean-Pierre Slakmon
Soquelec Limited
5757 Cavendish Boulevard, Suite 101
Montreal, Quebec
Canada H4W 2W8
Tel: 514-482-6427 / Fax: 514-482-1929
URL: http://www.soquelec.com



-----Original Message-----
} From: Mike Coviello [mailto:coviello-at-mae.uta.edu]
Sent: Monday, June 19, 2000 4:12 PM
To: listserver


Hi Y'all:
We are looking for a for-hire independent FIB company that has
experience in preparing TEM cross-sections of semiconductors. Please
contact me if you do this, or know of a lab that does.
Regards,
Michael Coviello
Lab Manager
Materials Science
University of Texas at Arlington




From daemon Mon Jun 19 18:23:34 2000



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Mon, 19 Jun 2000 18:00:27 -0400
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Hi Lee,
We follow a very similar protocol to yours with the exception that we use
propylene oxide rather than ethanol for infiltration.
PO : Spurrs 1:1 1 hour
PO : Spurrs 1:3 1- 2 hours
Spurrs 1 - 2 hours
Spurrs overnight (we do not infiltrate under vacuum)
fresh Spurrs 1 - 2 hours
polymerization at 60C 48 hours

We had the problem you describe when we tried to embed yeast in EPON
equivalents. Switching to Spurrs was the fix for us.
Frank

At 09:46 AM 6/19/00 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jun 19 18:33:38 2000



From: Jon Mulholland :      jwm-at-genome.stanford.edu
Date: Mon, 19 Jun 2000 16:23:07 -0700 (PDT)
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Hi Leona,
The standard way for dealing with yeast cells is to remove the
cell wall. This is done with glusulase (fancy name for snail guts) and or
lyticase (both from Sigma). If the wall cannot be removed for
experimental reasons (i.e. study of the plasma membrane/cell wall
interface) then you need to modify the carbohydrate linkages of the cell
wall to make the wall more permeable. This can be done by treating the
cells, after fixation, with 1% sodium metaperiodate for about 15 minutes.
However, most researchers simply wishing to examine yeast morphology
remove the wall because this not only improves inflitration of the resin
it also allows more extraction of the cytoplasm and thus makes it easier
to resolve structures and membranes within the ribosome rich yeast cell. A
classic protocol, by Byers and Goetsch, can be found in Vol 194, Methods
in Enzymology (AKA Guthrie and Fink) pg 602. I strongly encourage you to
read this article as yeast can be very problematic. You also want to use
the Hard Spurrs formulation and use 100% acetone (or propylene oxide) as
the last dehydration step before going into 1:1 resin. I have not
observed any difference in the ultrastructure of cells embedded in Spurrs
vs. polybed 812 (when the wall is removed).
If you want to do immuno-EM you might find our protocol useful;
checkout:
http://genome-www.stanford.edu/group/botlab/protocols/EM_protocol.pdf.

Jon Mulholland
Genetics Dept
Stanford University School of Medicine
Stanford, CA 94305-5120


On Mon, 19 Jun 2000, Leona Cohen-Gould wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
} I processed a series of pellets of yeast for a client using a glut-pfa fix,
} osmium, dehydration through ethanols, and a day & a half step-wise
} infiltration into Spurr's resin (1:1 with Ethanol for 4 hr, pure resin
} overnight under light vacuum, then fresh for 3 more hours, then embed in
} fresh). Polymerization was overnight at 60C. About half the blocks were
} soft and had to be returned to the oven for a prolonged polym. (over the
} weekend). I was able to get sections from each of the 10 samples, but in
} the 'scope, many of these looked a bit like swiss cheese. those that were
} not lacy exhibited areas where the resin pulled away from the cell coats of
} the yeasts. Clearly something went wrong with the
} infiltration/polymerization. I used the same batch of resin for other
} things, and its fine.
}
} Does anyone out there have experience with yeast? Any suggestions?
} I'd like to give this peson some usable data!
}
} Thanks in advance,
} Lee
}
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}
}



From daemon Tue Jun 20 07:07:44 2000



From: Keith Ryan :      kpr-at-ccms.ac.uk
Date: Tue, 20 Jun 2000 08:16:55 +0100
Subject: Re: Formvar & 1,2 dichloroethane

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Jonathan

Formvar in chloroform works well. I don't think we have ever tried
dochloroethane.

I have had to do the job myself recently and made a minor discovery -
the film seems to stick very well to acid washed slides! So, not so
cleverly clean. Then the second trick - float the film soon after it
has dried, within a minute. Then it seems to work better.

Keith

_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Tue Jun 20 07:07:45 2000



From: Ian MacLaren :      maclariz-at-yahoo.co.uk
Date: Tue, 20 Jun 2000 08:40:52 +0100 (BST)
Subject: RE: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
Thanks to all who responded to my enquiry and gave me useful advice concerning
the preparation of cross-sections of ceramic thin films.

Hopefully, I should now be able to prepare some better thin film specimens
using one or more of the suggestions that I received.

Thanks again

Best wishes

=====
Ian MacLaren
Beijing Laboratory of Electron Microscopy
Chinese Academy of Sciences, P.O. Box 2724
100080 Beijing
China
General Email: ian.maclaren-at-physics.org
Work (esp. large attachments): maclaren-at-image.blem.ac.cn

____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie



From daemon Tue Jun 20 07:07:46 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Tue, 20 Jun 2000 11:34:53 +0200 (MET DST)
Subject: SCIA2001, First Announcement and Call for Papers: (fwd)

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12th Scandinavian Conference
on Image Analysis
SCIA 2001

June 11-14, 2001 in Bergen, Norway

Sponsored by: IAPR (The International Association
for Pattern Recognition)
http://www.iapr.org

First Announcement and Call for Papers:

http://www.ux.his.no/scia2001/

Invitation to the 12th SCIA.
Following the previous conferences in Greenland, SCIA 2001 -
the 12th Scandinavian Conference on Image Analysis - will be
held in Bergen on the west coast of Norway. The conference is
arranged by the Norwegian Society for Image Processing and
Pattern Recognition (NOBIM) and sponsored by the International
Association for Pattern Recognition (IAPR). The conference
venue is Grieghallen, located in the city centre.

Scientific Program:
The conference will offer internationally acclaimed speakers in
plenary talks and parallel sessions with selected oral presentations
and posters. The conference language is English.
The different presentations will cover unpublished theoretical or
applied research results.

Invited Speakers:
Professor Theo Pavilidis, State University of New York at Stony
Brook, USA: "History of Image Analysis"
Professor Josef Begün, University of Halmstad, Sweden:
"Biometric Person Authentication"
Professor Matti Pietikäinen, University of Oulu, Finland:
"Machine Vision and Media Processing"
Associate Professor Torbjørn Eltoft, University of Tromsø, Norway:
"Neural Network approaches to Cluster-Detection-and-Labelling"


In addition, we are working to find an invited speaker for the
subject: "Images in the future mobile terminals".

Pre-conference Workshop/Tutorial:
A set of pre-conference half-day workshops/tutorials will be held
on June 11, 2001:
1. ICA (Independent Component Analysis): Professor Erkki Oja,
Helsinki University of Technology,
Finland.
2. Data fusion: Professor Jon Atli Benediktsson,
University of Iceland.

Conference topics:
* Image analysis
* Computer vision
* Pattern recognition
* Neural networks
* Statistical methods
* Industrial applications
* Multimedia
* Biomedical applications
* Remote sensing
* Future technologies

Paper submission and registration for presenting authors:
Only full papers in English will be accepted, and the length should
not exceed eight pages. All papers will be refereed by two
reviewers for publication in the conference proceedings.
Please send four copies of your paper to:

SCIA2001,
Department of Electrical and Computer Engineering,
Stavanger University College,
P.O.Box 2557 Ullandhaug,
N-4091 Stavanger,
Norway

Important dates:

Paper submission deadline: November 6, 2000
Notification of acceptance: January 19, 2001
*Camera-ready copy: March 19, 2001

*Camera-ready copy must be accompanied by registration
and payment by presenting author.
The cover page must contain:
* Title of the paper
* Name(s), complete address and e-mail for the author(s)
* Brief abstract (150-200 words)
* Keywords describing the main subject of the paper (3-5 words)

* Author's opinion on whether the paper is most suitable for oral
or poster presentation
* Name and address for correspondence

Papers considerably longer than the final size, risk being rejected.
The fee for one or two extra pages is NOK 500 per page.
The fee for colour illustrations is NOK 4000 per page.
The decision on oral or poster presentation will be taken solely on
suitability, not on paper quality. Paper submission information is
available at http://www.ux.his.no/scia2001/, where the LaTeX style
file and an example file in the recommended two-column LaTeX
format is available.

Enquires:
If you have scientific questions, please contact
Ivar.Austvoll-at-tn.his.no. The program with further information about
registration and payment will be send to you February 1, 2001.
If you occasionally have seen this announcement, and want to have
the program sent to you, please contact scia-at-plus-convention.no.

Program Committee:
Dr. Ivar Austvoll (Chairman), Norway
Prof. Jussi Parkkinen, Finland
Prof. Fritz Albregtsen, Norway
Dr. Alfred Hanssen, Norway
Prof. Gunilla Borgefors, Sweden
Dr. Anne Solberg, Norway
Dr. Bjarne Ersbøll, Danmark

Organized by: Norsk forening for bildebehandling og
mønstergjenkjenning (NOBIM)
(Norwegian Society for Image Processing and
Pattern Recognition) http://www.nobim.no/

SCIA2001 web site:

http://www.his.no/scia2001/ or http://www.ux.his.no/scia2001/





From daemon Tue Jun 20 12:43:50 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 20 Jun 2000 08:54:03 -0400
Subject: Re: Sperm Processing for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:42 PM -0500 6/19/0, Connie A Cummings wrote:

} Good day to all on the listserver,
}
} I have a person in my department who is interested in processing horse
} sperm for TEM. Does anyone who does this routinely be willing to give me
} some pointers as to how to process them? I've only worked with muscle and
} brain tissue so this is kinda new - I have processed cells from cell
} culture for TEM would it be the same procedure?
}
} Thanks so much,
} Connie Cummings, DVM
} Instructor Anatomic Pathology
} Department VBP
} Oklahoma State University
****************************
Connie,
Assuming that your colleague will bring you a semen sample, and that
orientation is not critical, you can spin the sperm to a pellet and treat
it like any other cell pellet. I've done various rodent, marsupial and
human sperm samples this way. Once at the microscope, you will have to
hunt around a bit to fine the appropriate views (head, mid-piece,
tails,etc), but since there are so many cells in the pellet, I've always
found what we wre looking for. Its the easiest way to go.
Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Tue Jun 20 12:43:51 2000



From: Paula Allan-Wojtas :      AllanWojtasP-at-Em.agr.ca
Date: Tue, 20 Jun 2000 10:09:25 -0400
Subject: power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,

I am posting this question to see if we can get some help for our building engineer to deal with problems we are having with our SEM (JEOL T330A).

We suspect that we are having a problem with the building power supply to our SEM. Other folks in our building using other types of equipment have found it necessary to use power conditioners or UPS systems to run their equipment - these systems seem to do the trick for them.

We are trying this approach with the power supply to our SEM, but find that there are some problems. When using the UPS system, we have to connect it up, put it on bypass, start up the SEM, then switch the UPS over after that - a real nuisance. If we don't do this, the SEM won't start.

Does anyone else have this problem, and if so, has found an easy solution? Please contact me offline if you have any suggestions and other words of wisdom. Thanks in advance. Also, thanks for all the help you as a group have given me when I posted previous questions - sometimes I forget to say thank you.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Tue Jun 20 12:43:51 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Tue, 20 Jun 2000 10:13:28 -0400
Subject: A Gatan User Meeting at M&M Philadelphia

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This is a preliminary message to all Gatan users who will be
attending the Microscopy and Microanalysis Meeting in Philadelphia
this August (13th to the 17th). I think it has become time to form a
User's Group to discuss the level of service and support that we are
receiving from Gatan. We should determine where the company should
be focusing its efforts and lobby them to correct problems that are
most important to us. Please let me know if you are interested in
attending such a meeting so I can gauge whether it should be held,
and how large a conference room I would need to reserve in Philly.

Note, Gatan representatives are encouraged to attend this meeting,
but it will be a user meeting run by the users.

I will not rant and rave here in a completely open forum, as I
believe it would be unfair. If you have concerns or comments on this
subject, whether or not you are attending M&M2000, please contact me
directly. Do NOT reply to the list, check the "To:" header before
sending your message, it should say "jfmjfm-at-engin.umich.edu" only.

Thank You.

John Mansfield.

Disclaimer: Opinions expressed in this message are my own personal
ones and do not represent necessarily those of my employers.

--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"



From daemon Tue Jun 20 12:43:54 2000



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Tue, 20 Jun 2000 11:52:26 -0400
Subject: acetone + LR Gold

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have experience taking specimens from acetone into LR Gold
resin? Some of the brochures on LR White seem to recommend against it, but
we are hoping it may be OK for LR Gold. We are doing freeze substitution
through acetone for EM-immunocytochemistry.

Thanks in advance for your help.
David H. Hall
Center for C. elegans Anatomy
Department of Neuroscience
1410 Pelham Parkway
Albert Einstein College of Medicine
Bronx, NY 10461

phone (718) 430-2195 FAX (718) 430-8821
hall-at-aecom.yu.edu
website: www.aecom.yu.edu/wormem



From daemon Tue Jun 20 12:53:57 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 20 Jun 2000 13:17:00 -0500
Subject: Re:power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
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Paula,

Sounds like the UPS capacity (VA) is sufficient to run the system, but under
rated for the start-up surge. One solution would be a higher capacity UPS.
One
sized to handle the surge load, however, could be quite large and expensive.

If the nature of your problems is related to line noise, but not voltage
levels,
you might consider an "ultra isolation transformer" and experiment with
various
grounding options to minimize interference.

If voltage flucuations are the problem, investigate a "ferro resonant"
transformer to stabilize the line. This transformer *should* be somewhat
more
economical than a similar capacity UPS. BEWARE: These devices produce a
loud
hum. You don't want it in the same room without some sort of noise
attenuation.


.Haven't checked relative pricing, but here is a typical link:
http://www.sola-hevi-duty.com/

Another posibility... Can you seperate the vacuum pump(s) supply from the
electronics, powering the pumps directly and using your UPS for only the
electronics? That may lessen the start-up load enough for the UPS to
funcion
normally.

Woody White
McDermott Technology, Inc.

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi, all,

I am posting this question to see if we can get some help for our building
engineer to deal with problems we are having with our SEM (JEOL T330A).

We suspect that we are having a problem with the building power supply to
our
SEM. Other folks in our building using other types of equipment have found
it
necessary to use power conditioners or UPS systems to run their equipment -
these systems seem to do the trick for them.

We are trying this approach with the power supply to our SEM, but find that
there are some problems. When using the UPS system, we have to connect it
up,
put it on bypass, start up the SEM, then switch the UPS over after that - a
real
nuisance. If we don't do this, the SEM won't start.

Does anyone else have this problem, and if so, has found an easy solution?
Please contact me offline if you have any suggestions and other words of
wisdom.
Thanks in advance. Also, thanks for all the help you as a group have given
me
when I posted previous questions - sometimes I forget to say thank you.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Tue Jun 20 16:07:22 2000



From: tellis2-at-hallmark.com
Date: Tue, 20 Jun 2000 13:56:33 -0500
Subject: EDS Window

Contents Retrieved from Microscopy Listserver Archives
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HI:
For the second year in a row I am having to have our thin window (low
element type-brand name with held) replaced.
We have the EDS connected to a TOPCON SM-500 environmental SEM. I look at a
lot industrial dusts for particle size and composition and the manuf. of
the EDS system says they poke holes in the polymer window.
Has anyone else have this problem?
I have elected to have a thin beryllium installed this time since my
boss is upset about spending $7000 every year plus the downtime and since
low element detection is not critical .
Thanks
Terry Ellis
Hallmark Cards Inc.



From daemon Tue Jun 20 16:07:23 2000



From: carla_aiwohi-at-usgs.gov
Date: Tue, 20 Jun 2000 13:37:19 -0700
Subject: TEM cell pellets in agarose

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

Does anyone have suggestions for encapsulating cell pellets in agarose?

I am working with flatfish cells but the pellets don't look cohesive enough
to withstand washing, dehydration etc.
We have some Type I agarose (Sigma, gel temp 36C, melting temp. 86C). I
plan to post-fix in 1% osmium tetroxide followed by 1% aqueous uranyl
acetate then embed in Spurr's resin.

As this is my first time working with cells, any help will be greatly
appreciated.

Thank you very much,
Carla Aiwohi
Western Fisheries Research Center
Seattle, WA



From daemon Tue Jun 20 16:07:24 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 20 Jun 2000 16:57:00 -0500
Subject: Re:EDS Window

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Terry,

The particulate (have also heard) can "shoot" holes in a thin window. My
former
SEM/EDS was an Etec with a LARGE roughing system and a turret detector with
Be,
UTW, and "open" positions. Both windows lasted the 15 odd years it was in
use
before replacement. An important point is to evacuate and vent slowly so as
to
not accelerate the particles into the window.

I added a manual valve to the Etec between the roughing system and the
chamber.
With the automatic valves closed, I could slowly open the manual valve to
rough
the chamber down to a point where any particulate is not disturbed, close
the
valve, then switch to automatic to finish the evacuation. Venting gas was
from
my cryo of liquid nitrogen (pretty dry!) which I pressure/flow controlled
for
similar results on venting.

My modification was driven by the need to avoid damage to fluffy ceramic
specimens, but worked well to protect the detector also.

Have not added such a feature to the new SEM/Thin Window EDS system, but
(for
now) the chamber is pristine and the fluffy specimens are not part of the
work
mix.

Woody White
McDermott Technology, Inc

------------------------------------------------------------------------
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HI:
For the second year in a row I am having to have our thin window (low
element type-brand name with held) replaced.
We have the EDS connected to a TOPCON SM-500 environmental SEM. I look at a
lot industrial dusts for particle size and composition and the manuf. of
the EDS system says they poke holes in the polymer window.
Has anyone else have this problem?
I have elected to have a thin beryllium installed this time since my
boss is upset about spending $7000 every year plus the downtime and since
low element detection is not critical .
Thanks
Terry Ellis
Hallmark Cards Inc.



From daemon Tue Jun 20 17:58:18 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 20 Jun 2000 15:50:36 -0700
Subject: Re: power problems with the SEM

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Hi,

My guess is that the SEM inititally draws power that exceeds the ratings of the UPS.
It is probably due to the rotary pumps that can take up to ten amps upon start up.

The alternatives are to increase the power rating of the UPS or rewire the rotary pump so it draws it's power from the line and not through the UPS (the pumps is not that sensitive anyway).

Good Luck,

Earl weltmer

Paula Allan-Wojtas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, all,
}
} I am posting this question to see if we can get some help for our building engineer to deal with problems we are having with our SEM (JEOL T330A).
}
} We suspect that we are having a problem with the building power supply to our SEM. Other folks in our building using other types of equipment have found it necessary to use power conditioners or UPS systems to run their equipment - these systems seem to do the trick for them.
}
} We are trying this approach with the power supply to our SEM, but find that there are some problems. When using the UPS system, we have to connect it up, put it on bypass, start up the SEM, then switch the UPS over after that - a real nuisance. If we don't do this, the SEM won't start.
}
} Does anyone else have this problem, and if so, has found an easy solution? Please contact me offline if you have any suggestions and other words of wisdom. Thanks in advance. Also, thanks for all the help you as a group have given me when I posted previous questions - sometimes I forget to say thank you.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca



From daemon Tue Jun 20 22:15:09 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 20 Jun 2000 21:22:29 -0500
Subject: Administrivia: Microscopy Listserver Search Engine Now On-Line

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Colleagues....

A search engine for the Microscopy Listserver Archives is now on-line.

You can access it throught the Listserver Home Page at:

http://www.msa.microscopy.com/MicroscopyListserver

You can search the entire ~ 7 year history of the Listserver
if your heart desires...

Search options allow you to search the Subject, Author, and Message Text
seperately or together.

Please let me know of any problems, since I wrote the search engine I'll
have to fix it when it breaks.

It's not perfect, but hey it works....

Cheers...

Nestor
Your Friendly Neighborhood SysOp




From daemon Tue Jun 20 22:15:11 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 20 Jun 2000 22:53:54 -0400
Subject: Speaking of User group meetings - How about Emispec?

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John Mansfield's reminded me that I would like to have some sort of users'
group meeting for Emispec users for mutual support and information exchange.
I have discussed this with the Emispec folks, but I don't think that they
have followed up on it. Could I seed some level of support for a
get-together at M&M MM so that I can send it to Emispec. Please respond to
me offline and I will send them the number and names of the people that
would desire something like that. I would like to see something in a
positive and instructive type of meeting.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Wed Jun 21 08:34:26 2000



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Wed, 21 Jun 2000 15:32:31 +1100
Subject: Rotory pump oil

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

this is a message for EM people in Sydney or Woolongong, Australia.

The rotory pump on my JEOL 2020F TEM is short of oil and making a bit of
noise so I'm trying to find some quickly. Our JEOL service guys have
ordered some "MR100" but it may take a couple of weeks to arrive. Can any
one lend me a few hundred mls in the meantime?

Can anyone suggest a local supplier of this rotory pump oil?

Cheers,


Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.




From daemon Wed Jun 21 08:34:27 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 20 Jun 2000 17:49:44 +0100
Subject: Re: power problems with the SEM

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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

My guess would be the current loading at start up. When you turn on
an SEM from cold, everything starts and draws current. In particular,
the rotary pump kicks in and draws a high current as it starts. You
will need to be able to set up the UPS so that it can handle this -
or set it on a short timer so that it automatically switches in, say,
10 mins after start up.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Wed Jun 21 08:34:31 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 21 Jun 2000 09:16:35 +0200 (METDST)
Subject: Re: power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Probably you are having problems with the high switch on current the sem
draws. You could try switching on different parts of the SEM system not at
the same time (if possible). You could also consider to only power the
electronics of the SEM via the UPS, in that way you reduce the required
current drastically, while there is little use for a pump to sit on the
UPS power.
Alternatively you could look for a more powerfull UPS that can handle the
larger currents (for short moment is enough- see data sheets of different
UPS's).

Hope this helps...

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************

On Tue, 20 Jun 2000, Paula Allan-Wojtas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, all,
}
} I am posting this question to see if we can get some help for our building engineer to deal with problems we are having with our SEM (JEOL T330A).
}
} We suspect that we are having a problem with the building power supply to our SEM. Other folks in our building using other types of equipment have found it necessary to use power conditioners or UPS systems to run their equipment - these systems seem to do the trick for them.
}
} We are trying this approach with the power supply to our SEM, but find that there are some problems. When using the UPS system, we have to connect it up, put it on bypass, start up the SEM, then switch the UPS over after that - a real nuisance. If we don't do this, the SEM won't start.
}
} Does anyone else have this problem, and if so, has found an easy solution? Please contact me offline if you have any suggestions and other words of wisdom. Thanks in advance. Also, thanks for all the help you as a group have given me when I posted previous questions - sometimes I forget to say thank you.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}
}
}



From daemon Wed Jun 21 08:34:32 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 21 Jun 2000 08:36:37 +0100 (BST)
Subject: Re: EDS Window

Contents Retrieved from Microscopy Listserver Archives
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Hi Terry,

If you are using water vapour in your environmental SEM you may
want to check the suitability of a Be window. I have a vague memory that
water will make holes in the thin (6-8um) Be, check it out with your
supplier.

I have used two polymer windows on an EDX detector in a TEM
gas reaction cell. The front window is on a replaceable mount in front of
the detector. This is easily exchangeable in case I cover it with reaction
products from the in-situ experiment; I don't want to see them in all
subsequent spectra. Much cheaper then a window replacement and can be
carried out by me on site. Contact me for further details if you want to.

Reducing the disturbance to the vacuum system gasses during
pumpdown and venting by limiting the speed may help prevent dust particles
damaging the window in the first place.

Regards,
Ron

On Tue, 20 Jun 2000 tellis2-at-hallmark.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} HI:
} For the second year in a row I am having to have our thin window (low
} element type-brand name with held) replaced.
} We have the EDS connected to a TOPCON SM-500 environmental SEM. I look at a
} lot industrial dusts for particle size and composition and the manuf. of
} the EDS system says they poke holes in the polymer window.
} Has anyone else have this problem?
} I have elected to have a thin beryllium installed this time since my
} boss is upset about spending $7000 every year plus the downtime and since
} low element detection is not critical .
} Thanks
} Terry Ellis
} Hallmark Cards Inc.
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Wed Jun 21 08:34:39 2000



From: Your Name :      KMG1TEX-at-bolton.ac.uk
Date: Wed, 21 Jun 2000 12:36:19 +0100
Subject: student requires information

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir/Madam

I am currently studying for an MSc in Textile studies, at Bolton
Institute, England.

For part of my studies I have been analysing some polyester film that
has been treated with 10% sodium hydroxide under imposed load under
polarized light on an optical microscope. Vivid colours have been noted,
Reds, greens, and I am struggling to find information to outline what
these colours are actually indicating. I therefore write and ask for any
information you may deem relevant, I would be extremely grateful for.

I thank you for your time.

Yours Faithfully

Kelly Goodman

EMAIL: suite666-at-netscapeonline.co.uk



From daemon Wed Jun 21 08:34:40 2000



From: Leroux christine :      leroux-at-univ-tln.fr
Date: Wed, 21 Jun 2000 14:07:10 +0200
Subject: EDS analysis of Co in WC-Co

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Hi,
I am working on powders of mixed WC and Co and I have some problems with EDS.
EDS analysis on grains whose diffractions patterns are unambiguously
indexed in the Co structure give a majority of W, and EDS analysis on
grains whose diffractions patterns are unambiguously indexed in the WC
structure (without distortion and superstructures) always indicate the
presence of Co (with a majority of W). So I was wondering if there could be
any problem with EDS on Cobalt (because of magnetism????)

christine

**************************************
Christine leroux
Lab. M.M.I
Universite de Toulon-Var, Bat.R
B.P.132
F-83957 La Garde Cedex
tel: 00 33 (0) 4 94 14 25 07
fax: 00 33 (0) 4 94 14 21 68

**************************************



From daemon Wed Jun 21 08:44:49 2000



From: Robert.Carlton-at-aventis.com
Date: Wed, 21 Jun 2000 08:35:34 -0500
Subject: Re: EDS Window

Contents Retrieved from Microscopy Listserver Archives
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Terry,

I have an Electroscan 2010 and routinely have problems with the window. I
have ascribed it to particles flying around from the surface of the sample.
This generally occurs at startup when there may be some charging. We solved
the problem by retrofiting the detector so that it can be retracted from the
chamber when changing specimens. Its a pain in the butt, but less so than
having to replace windows.

Thanks
Robert Carlton
Aventis Pharmaceuticals
robert.carlton-at-aventis.com




From daemon Wed Jun 21 17:28:58 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 21 Jun 2000 09:06:04 -0500
Subject: Re: student ... (LM:polarized light)

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Greetings,
Yes, those colors are beautiful, arn't they?
The colors indicate the magnitude of birefringent retardation
in your sample. In essence, you are seeing subtraction colors
resulting from the diminution of intensity at certain wavelengths.
Say your sample has a retardation of 550 nm. When linearly polarized
light of that wavelength passes through the sample, it will be
retarded by exactly one wavelength, which is the same as zero
retardation. Thus, it will not be affected by the sample and will be
blocked by the analyzer. But light of longer or shorter wavelengths
will be retarded by more or less than a wave and so will emerge as
elliptically polarized light and thus will have a component
transmitted through the analyzer.
So for any actual sample retardation, the color will result
from exactly how much of each wavelength gets through. Because our
eyes are very sensitive to color, this has been used for more than
100 years to measure/estimate retardation. There is a chart that
reproduces the colors as a function of retardation. I have no idea if
this chart has made it on line but I would be careful. The colors are
very tricky to print and great care was used to get them right. You
would do far better to look this one up in your library. I am sure
the Bolton Textile Institute will have excellent books on polarized
light microscopy, and while they might be dusty, this particular
corner of science has been well understood for years and years.
Hope this helps,
Tobias Baskin



}
}
} Dear Sir/Madam
}
} I am currently studying for an MSc in Textile studies, at Bolton
} Institute, England.
}
} For part of my studies I have been analysing some polyester film that
} has been treated with 10% sodium hydroxide under imposed load under
} polarized light on an optical microscope. Vivid colours have been noted,
} Reds, greens, and I am struggling to find information to outline what
} these colours are actually indicating. I therefore write and ask for any
} information you may deem relevant, I would be extremely grateful for.
}
} I thank you for your time.
}
} Yours Faithfully
}
} Kelly Goodman
}
} EMAIL: suite666-at-netscapeonline.co.uk

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed Jun 21 17:29:02 2000



From: Sue Danielson :      sdaniels-at-post.its.mcw.edu
Date: Wed, 21 Jun 2000 10:11:03 -0500
Subject: TEM-micrographs for teaching manual

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Hello everyone,

A colleage of mine here at the Medical College of Wisconsin is working on a
new teaching manual for our first-year medical students' cells & tissues
course. We are currently putting together a portion of the manual which
shows students electron micrographs of cells and organelles. We are looking
for high quality TEM images (non-copyrighted) of structures such as the
following:

Cytoskeleton (actin microfilaments, microvilli, microtubules, centrioles)

Cell Membrane (particularly intercellular junctions such as z. occludens,
adherens, desmosomes)

Protein synthesis & vescicles ( ribosomes, RER, golgi, endosomes,
lysosomes, peroxisomes, & vescicles-coated, endocytotic, secretory)

Nucleus (nuclear envelope, nuclear pores, chromatin, nucleolus)

MItochondria

We would be grateful to anyone who feels they have something useful to
contribute. The plan is to scan appropriate images for the lab manual and
return them as soon as possible to the owner. There will be a list at the
end of the manual acknowledging all contributors.

Thank you everyone,

Susan K. Danielson, MS
Neuromuscular Lab Coordinator
Dept. Neurology, Medical College of Wisconsin
ph: 414.259.3836
email: sdaniels-at-mcw.edu








From daemon Wed Jun 21 17:29:07 2000



From: Stephen Page :      stevep-at-micrometsys.com
Date: Wed, 21 Jun 2000 13:17:43 -0500
Subject: Job Posting-Technical Representative

Contents Retrieved from Microscopy Listserver Archives
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Here we grow again! We need more help.

We are looking for qualified professionals to provide applications-based
sales and technical support for our complete line of optical microscopes,
sample preparation equipment, consumables, and digital imaging systems in
the Dallas/Ft. Worth and Austin, TX areas. Must have at least 2 years
experience in failure analysis or materials analysis using microscopes and
sample preparation equipment. Prior sales experience is not necessary.
Understanding of dimensional measurement and image analysis systems a plus.

Please respond by e-mail to mms-at-micrometsys.com



From daemon Wed Jun 21 17:29:07 2000



From: mms :      mms-at-micrometsys.com
Date: Wed, 21 Jun 2000 13:21:38 -0500
Subject: Job Posting-Technical Representative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here we grow again! We need more help.

We are looking for qualified professionals to provide applications-based
sales and technical support for our complete line of optical microscopes,
sample preparation equipment, consumables, and digital imaging systems in
the Dallas/Ft. Worth and Austin, TX areas. Must have at least 2 years
experience in failure analysis or materials analysis using microscopes and
sample preparation equipment. Prior sales experience is not necessary.
Understanding of dimensional measurement and image analysis systems a plus.

Please respond by e-mail to mms-at-micrometsys.com



From daemon Wed Jun 21 17:29:08 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Wed, 21 Jun 2000 13:42:12 -0500 (CDT)
Subject: Re: acetone + LR Gold

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Responding to the message of
{3.0.5.32.20000620115226.008c7a80-at-mailserver.aecom.yu.edu}
from "David H. Hall" {hall-at-aecom.yu.edu} :

David,

I've done it, but the thing to watch out for is that if the temperature of your
acetone/LR Gold mixtures - say 1:1 acetone:LR Gold - drops below about -35 C,
some components of the LR Gold will start to freeze out, solutions gets cloudy.
For methanol sub-solution's, LR Gold starts to freeze out at even higher temps,
about -27 C.

Just experiment with your sub mixtures at various temperatures to see if you get
any freeze-out like this happening, before you do an actual sub run. If so just
make sure you stay warmer than freezing points.

I'm curious why this happens, anyone else got any info on this?

Gib Ahlstrand



} Does anyone have experience taking specimens from acetone into LR Gold
} resin? Some of the brochures on LR White seem to recommend against it, but
} we are hoping it may be OK for LR Gold. We are doing freeze substitution
} through acetone for EM-immunocytochemistry.
}
} Thanks in advance for your help.
} David H. Hall
} Center for C. elegans Anatomy
} Department of Neuroscience
} 1410 Pelham Parkway
} Albert Einstein College of Medicine
} Bronx, NY 10461
}
} phone (718) 430-2195 FAX (718) 430-8821
} hall-at-aecom.yu.edu
} website: www.aecom.yu.edu/wormem


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Wed Jun 21 17: